usp20 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp20
Entrez Gene ID	393962
Full Name	ubiquitin specific peptidase 20
Synonyms	im:7151955,zgc:63661
General protein information	Preferred Names ubiquitin specific peptidase 20 Names ubiquitin carboxyl-terminal hydrolase 20 deubiquitinating enzyme 20 ubiquitin specific protease 20 ubiquitin thioesterase 20 ubiquitin thiolesterase 20 ubiquitin-specific-processing protease 20
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301457.1	XP_009299732.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
XM_009301458.2	XP_009299733.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X2
XM_009301457.2	XP_009299732.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
NM_200987.2	NP_957281.2	ubiquitin carboxyl-terminal hydrolase 20
XM_009301458.3	XP_009299733.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Usp20	NP_083122.1
Macaca mulatta (Rhesus monkey)	USP20	XP_001107144.1
Canis lupus familiaris (dog)	USP20	XP_537816.3
Bos taurus (cattle)	USP20	NP_001098803.1
Gallus gallus (chicken)	USP20	XP_004945924.1
Danio rerio (zebrafish)	usp20	NP_957281.2
Drosophila melanogaster (fruit fly)	CG8494	NP_610943.2
Xenopus tropicalis (tropical clawed frog)	usp20	NP_001090641.1
Homo sapiens (human)	USP20	NP_001008563.2
Pan troglodytes (chimpanzee)	USP20	XP_520313.2
Rattus norvegicus (Norway rat)	Usp20	NP_001101297.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003821	XP_310382.7

Gene Ontology
Bibliography

Related articles in PubMed

Importance of deubiquitinases in zebrafish craniofacial development.
Tse WKF
Biochemical and biophysical research communications487(4)813-819(2017 06)

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

Positive correlation between gene coexpression and positional clustering in the zebrafish genome.
Ng YK, Wu W, Zhang L
BMC genomics1042(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following usp20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02974	NM_200987.2 Latest version!	Danio rerio ubiquitin specific peptidase 20 (usp20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa10428	XM_009301457.2 Latest version!	Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa40597	XM_009301458.3 Latest version!	Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa10428	XM_009301457.1	Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa40597	XM_009301458.2	Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp20 ( NM_200987.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957281.2 Danio rerio ubiquitin specific peptidase 20 (usp20), mRNA.

Price & Availability↑

CloneID	ODa02974
Clone ID Related Accession (Same CDS sequence)	NM_200987.2
Accession Version	NM_200987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529683200000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR382323.8. On or before Nov 2, 2007 this sequence version replaced XM_686993.2, NM_200987.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACTGACA GCGGGGATCT TTGTCCTCAC TTGGACTCGA TTGGTGAAGT TACTAAAGAA 
GAACTGATTC AGAAATCAAA GGGCACGTGT CAGTCATGTG GAGTAGGTGG ACCAAATCTC 
TGGGCATGTC TGCAGTGTGA CTGTCCTTAT GTAGGCTGTG GAGAGTCGTA TTCAGACCAC 
AGTACTATTC ATGCACAGGC CAAAAAGCAC AATCTGACAG TAAACCTGAC CACATTTAGG 
GTGTGGTGTT ATGTCTGCGA AAGAGAGGTG TTTCTGGAGC CAAAGCCTGT TACTCCTGTA 
TCATCAGCTC ACCGCTGCAA ACCACATGAC CAGGATCCTG TTTTGCAGAC TACATGTTAC 
CCATTGAAGG CGGTGCCTAT CGCAGTGGCA GATGAGGAGG GCTCAGAGTC TGAGGAGGAT 
GAGCTAAAAC CTAGAGGTCT GACAGGGATG AAAAACATTG GAAACTCTTG TTATATGAAT 
GCTGCACTGC AGGCCTTGTC CAACTGTCCT CCTTTGACTC AGTTTTTCCA GGATTGCAGT 
GGATTGGTGC GTACGGATAA GAAGCCGGCA TTGTGTAAAA GCTATCAGAA GCTTATCTCT 
GAACTCTGGC ACAAGAAACG GCCGAGTTAT GTTGTGCCAA CTACTCTGTT TCATGGAATC 
AAACTGGTGA ACCCCATGTT TCGTGGCTAT GCTCAACAGG ACACTCAGGA GTTTTTGCGA 
TGTCTGATGG ACCAGCTTCA CGAGGAGCTG AAGGAGCCTT TGTTTGACTG CAGCGGTGGC 
ATCTCTGAAG TCGAGCCTGA CCTCAGTCTG GACAGCTGTA ATCTTGTGGA CGGAGACCGA 
AGCCCCTCTG AAGACGAATT CCTGTCGTGT GACTCTGGAT CAGGAAGCGA AAGGGGAGAT 
GGAGAGCGTG CAGGAGGAGA AGCAGAGCTG CTCATTCAAG ATGAATGTGT GGCAGTGAGA 
GGAACCGGAG GCATCTCGGA GAAAGAGAGG CTGAAGGAGA GAAGAGGAGA AGAGAGGACA 
CGTGAAATGG ATGAGGATGC AGATGTGGAC ACAGCTGCAC AAGACGGACA GGCTGAAAGA 
GAAACTGAAA CAGCGACACC AGCCACAGCT GTGCCGGCAC CAGGAAACAC AGAACCTGAT 
AATGAAGCTT CCATGCACTG TCCATCCTCT CGCCCTTGTA GCCCCGCTCA CAGTGTACAG 
GAGCTTCACT CCAGACTCTC TAGTAACCCA CCAAGATCCA GCCCTCTCAG AACTGGACCC 
ACGTATACTT TCAAGAAAGC TCAGATTCTA CTGAGCACCA AGAAGAAGAA GCAGTCCCGT 
TTCCGTAGTG TTATTTCCGA TATCTTTGAT GGATCCATTC TGAGTCTTGT TCAGTGTTTG 
ACATGTGACC GGGTTTCGAC AACTGTGGAG ACGTTTCAGG ACCTTTCTTT GCCTATACCC 
GGTAAAGAAG ATTTGGCTAA GCTTCATTCC TCCATTCACC AGAGTGCACC TGTGAAAGCT 
GGTGTCTGCA CTGATGGCTA TGCTGCACAG GGCTGGATCT CCTACATCAT GGACTCTATA 
CGAAGGTTTG TAGTGTCCTG TATTCCAAGT TGGTTTTGGG GCCCCATGGT AACGCTAGAG 
GACTGTCTGG CAGCTTTCTT TGCTGCTGAT GAGCTGAAAG GAGACAACAT GTATAGCTGT 
GAACGGTGTA AAAAATTAAG AAATGGTGTG AAATATTGTA AAGTTCTGCG TCTACCAGAA 
ATATTGTGCA TTCACCTGAA GCGCTTCAGA CATGAAGTCA TGTACTCGTT CAAAATAAAT 
AGCCACGTGT CATTCCCATT GGAGGGTTTG GATCTCAAAC CGTTCCTGGC TAAGGAAAGC 
CCGTCCCAGA TTACTACCTA TGACCTGCTG TCTGTCATCT GTCATCACGG CACTGCTGGA 
AGTGGGCATT ACATAGCGTA CTGCCAGAAT GTGATCAATG GACAGTGGTA CGAGTTTGAC 
GATCAGTATG TAACAGAAGT GCACGAGACT GTAGTCCAGA ATGCTGAAGC ATATGTCCTA 
TTCTACAGGA AGAGCAGTGA AGAATCTGTG AGAGAAAGAC AAAGGGTTGT CGCTCTGGCT 
AATTTGAAGG AGCCCAGTTT GCTGCAGTTC TACATCTCCA GAGAGTGGCT CAACAAATTC 
AACACATTCA CTGAGCCCGG ACCCATCACC AATCACACTT TCCTTTGTCA GCACGGAGGT 
ACTCGAATTC CTCCTACAAA GTACCATTAT GTGGATGATC TGGTTGTGAT CCTTCCTCAA 
AATGTGTGGG AATATCTCTA CAACAGGTTT GGAGGTGGCC CGGCTGTGAA TCACTTATAT 
GTGTGTGCAA TTTGCCAAGT GGAGATCGAG ACCTTAGCCA AACGACGGAA ACTGGAGATA 
GACACCTTCA TTAAGTTAAA TAAGGAGTTT CAGGCTGAAG AAGCTCCTAC CGTCATCCTT 
TGTATAAGCA TGCAGTGGTT CAGAGAATGG GAGAACTTTG TCAAGGGCAA AGACAATGAG 
CCACCAGGCC CCATTGACAA CAGCAAGATT GCAGTAATGA AAGGCGGTCA TATCCAGCTT 
AAACAAGGAG CAGACTATGG GCAGATATCT GAAGAAACGT GGCAGTACTT GTTAAGTATT 
TATGGCGGTG GGCCAGAGAT TGCAGTGCGG CAAACCATCA GCCCCCCAGA CACTGACACA 
CACGGGGAGA GGAAAATAGA GGCAGAGACC AGAGCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957281.2
CDS	1..2742
Translation	MTDSGDLCPHLDSIGEVTKEELIQKSKGTCQSCGVGGPNLWACLQCDCPYVGCGESYSDHSTIHAQAKKHNLTVNLTTFRVWCYVCEREVFLEPKPVTPVSSAHRCKPHDQDPVLQTTCYPLKAVPIAVADEEGSESEEDELKPRGLTGMKNIGNSCYMNAALQALSNCPPLTQFFQDCSGLVRTDKKPALCKSYQKLISELWHKKRPSYVVPTTLFHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPLFDCSGGISEVEPDLSLDSCNLVDGDRSPSEDEFLSCDSGSGSERGDGERAGGEAELLIQDECVAVRGTGGISEKERLKERRGEERTREMDEDADVDTAAQDGQAERETETATPATAVPAPGNTEPDNEASMHCPSSRPCSPAHSVQELHSRLSSNPPRSSPLRTGPTYTFKKAQILLSTKKKKQSRFRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSSIHQSAPVKAGVCTDGYAAQGWISYIMDSIRRFVVSCIPSWFWGPMVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLKPFLAKESPSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEAYVLFYRKSSEESVRERQRVVALANLKEPSLLQFYISREWLNKFNTFTEPGPITNHTFLCQHGGTRIPPTKYHYVDDLVVILPQNVWEYLYNRFGGGPAVNHLYVCAICQVEIETLAKRRKLEIDTFIKLNKEFQAEEAPTVILCISMQWFREWENFVKGKDNEPPGPIDNSKIAVMKGGHIQLKQGADYGQISEETWQYLLSIYGGGPEIAVRQTISPPDTDTHGERKIEAETRAL

Target ORF information:

RefSeq Version	NM_200987.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 20 (usp20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200987.2

ORF Insert Sequence:

ATGACTGACA GCGGGGATCT TTGTCCTCAC TTGGACTCGA TTGGTGAAGT TACTAAAGAA 
GAACTGATTC AGAAATCAAA GGGCACGTGT CAGTCATGTG GAGTAGGTGG ACCAAATCTC 
TGGGCATGTC TGCAGTGTGA CTGTCCTTAT GTAGGCTGTG GAGAGTCGTA TTCAGACCAC 
AGTACTATTC ATGCACAGGC CAAAAAGCAC AATCTGACAG TAAACCTGAC CACATTTAGG 
GTGTGGTGTT ATGTCTGCGA AAGAGAGGTG TTTCTGGAGC CAAAGCCTGT TACTCCTGTA 
TCATCAGCTC ACCGCTGCAA ACCACATGAC CAGGATCCTG TTTTGCAGAC TACATGTTAC 
CCATTGAAGG CGGTGCCTAT CGCAGTGGCA GATGAGGAGG GCTCAGAGTC TGAGGAGGAT 
GAGCTAAAAC CTAGAGGTCT GACAGGGATG AAAAACATTG GAAACTCTTG TTATATGAAT 
GCTGCACTGC AGGCCTTGTC CAACTGTCCT CCTTTGACTC AGTTTTTCCA GGATTGCAGT 
GGATTGGTGC GTACGGATAA GAAGCCGGCA TTGTGTAAAA GCTATCAGAA GCTTATCTCT 
GAACTCTGGC ACAAGAAACG GCCGAGTTAT GTTGTGCCAA CTACTCTGTT TCATGGAATC 
AAACTGGTGA ACCCCATGTT TCGTGGCTAT GCTCAACAGG ACACTCAGGA GTTTTTGCGA 
TGTCTGATGG ACCAGCTTCA CGAGGAGCTG AAGGAGCCTT TGTTTGACTG CAGCGGTGGC 
ATCTCTGAAG TCGAGCCTGA CCTCAGTCTG GACAGCTGTA ATCTTGTGGA CGGAGACCGA 
AGCCCCTCTG AAGACGAATT CCTGTCGTGT GACTCTGGAT CAGGAAGCGA AAGGGGAGAT 
GGAGAGCGTG CAGGAGGAGA AGCAGAGCTG CTCATTCAAG ATGAATGTGT GGCAGTGAGA 
GGAACCGGAG GCATCTCGGA GAAAGAGAGG CTGAAGGAGA GAAGAGGAGA AGAGAGGACA 
CGTGAAATGG ATGAGGATGC AGATGTGGAC ACAGCTGCAC AAGACGGACA GGCTGAAAGA 
GAAACTGAAA CAGCGACACC AGCCACAGCT GTGCCGGCAC CAGGAAACAC AGAACCTGAT 
AATGAAGCTT CCATGCACTG TCCATCCTCT CGCCCTTGTA GCCCCGCTCA CAGTGTACAG 
GAGCTTCACT CCAGACTCTC TAGTAACCCA CCAAGATCCA GCCCTCTCAG AACTGGACCC 
ACGTATACTT TCAAGAAAGC TCAGATTCTA CTGAGCACCA AGAAGAAGAA GCAGTCCCGT 
TTCCGTAGTG TTATTTCCGA TATCTTTGAT GGATCCATTC TGAGTCTTGT TCAGTGTTTG 
ACATGTGACC GGGTTTCGAC AACTGTGGAG ACGTTTCAGG ACCTTTCTTT GCCTATACCC 
GGTAAAGAAG ATTTGGCTAA GCTTCATTCC TCCATTCACC AGAGTGCACC TGTGAAAGCT 
GGTGTCTGCA CTGATGGCTA TGCTGCACAG GGCTGGATCT CCTACATCAT GGACTCTATA 
CGAAGGTTTG TAGTGTCCTG TATTCCAAGT TGGTTTTGGG GCCCCATGGT AACGCTAGAG 
GACTGTCTGG CAGCTTTCTT TGCTGCTGAT GAGCTGAAAG GAGACAACAT GTATAGCTGT 
GAACGGTGTA AAAAATTAAG AAATGGTGTG AAATATTGTA AAGTTCTGCG TCTACCAGAA 
ATATTGTGCA TTCACCTGAA GCGCTTCAGA CATGAAGTCA TGTACTCGTT CAAAATAAAT 
AGCCACGTGT CATTCCCATT GGAGGGTTTG GATCTCAAAC CGTTCCTGGC TAAGGAAAGC 
CCGTCCCAGA TTACTACCTA TGACCTGCTG TCTGTCATCT GTCATCACGG CACTGCTGGA 
AGTGGGCATT ACATAGCGTA CTGCCAGAAT GTGATCAATG GACAGTGGTA CGAGTTTGAC 
GATCAGTATG TAACAGAAGT GCACGAGACT GTAGTCCAGA ATGCTGAAGC ATATGTCCTA 
TTCTACAGGA AGAGCAGTGA AGAATCTGTG AGAGAAAGAC AAAGGGTTGT CGCTCTGGCT 
AATTTGAAGG AGCCCAGTTT GCTGCAGTTC TACATCTCCA GAGAGTGGCT CAACAAATTC 
AACACATTCA CTGAGCCCGG ACCCATCACC AATCACACTT TCCTTTGTCA GCACGGAGGT 
ACTCGAATTC CTCCTACAAA GTACCATTAT GTGGATGATC TGGTTGTGAT CCTTCCTCAA 
AATGTGTGGG AATATCTCTA CAACAGGTTT GGAGGTGGCC CGGCTGTGAA TCACTTATAT 
GTGTGTGCAA TTTGCCAAGT GGAGATCGAG ACCTTAGCCA AACGACGGAA ACTGGAGATA 
GACACCTTCA TTAAGTTAAA TAAGGAGTTT CAGGCTGAAG AAGCTCCTAC CGTCATCCTT 
TGTATAAGCA TGCAGTGGTT CAGAGAATGG GAGAACTTTG TCAAGGGCAA AGACAATGAG 
CCACCAGGCC CCATTGACAA CAGCAAGATT GCAGTAATGA AAGGCGGTCA TATCCAGCTT 
AAACAAGGAG CAGACTATGG GCAGATATCT GAAGAAACGT GGCAGTACTT GTTAAGTATT 
TATGGCGGTG GGCCAGAGAT TGCAGTGCGG CAAACCATCA GCCCCCCAGA CACTGACACA 
CACGGGGAGA GGAAAATAGA GGCAGAGACC AGAGCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp20 ( XM_009301457.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299732.1 Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10428
Clone ID Related Accession (Same CDS sequence)	XM_009301457.1 , XM_009301457.2
Accession Version	XM_009301457.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGTCA CGCTCCTGAT GTATTGTTTG GATGTGCCTG TGCTTTGTTT CAAACAGAGG 
AAGATTGTGT TGACATCTGA GAGTGAGAAG AAAATCAGGA TGACTGACAG CGGGGATCTT 
TGTCCTCACT TGGACTCGAT TGGTGAAGTT ACTAAAGAAG AACTGATTCA GAAATCAAAG 
GGCACGTGTC AGTCATGTGG AGTAGGTGGA CCAAATCTCT GGGCATGTCT GCAGTGTGAC 
TGTCCTTATG TAGGCTGTGG AGAGTCGTAT TCAGACCACA GTACTATTCA TGCACAGGCC 
AAAAAGCACA ATCTGACAGT AAACCTGACC ACATTCAGGG TGTGGTGTTA TGTCTGCGAA 
AGAGAGGTGT TTCTGGAACC GAAGCCTGTT ACTCCTGTAT CATCAGCTCA CCGCTGCAAA 
CCACATGACC AGGATCCTGT TTCGCAGACT ACATGTTACC CATTGAAGGC GGTGCCTATC 
GCAGTGGCAG ATGAGGAGGG CTCAGAGTCT GAGGAGGATG AGCTCAAACC TAGAGGTCTG 
ACAGGGATGA AAAACATTGG AAACTCTTGT TATATGAATG CTGCACTGCA GGCCTTGTCC 
AACTGTCCTC CTTTGACTCA GTTTTTCCAG GATTGCAGTG GATTGGTGCG TACGGATAAG 
AAGCCGGCAT TGTGTAAAAG CTATCAGAAG CTTATCTCTG AACTCTGGCA CAAGAAACGG 
CCGAGTTATG TTGTGCCAAC TACTCTGTTT CATGGAATCA AACTGGTGAA CCCCATGTTT 
CGTGGCTATG CTCAACAGGA CACTCAGGAG TTTTTGCGAT GTCTGATGGA CCAGCTTCAC 
GAGGAGCTGA AGGAGCCTTT GTTTGACTGC AGCGGTGGCA TCTCTGAAGT CGAGCCTGAC 
CTCAGTCTGG ACAGCTGTAA TCTTGTGGAC GGAGACCGAA GCCCCTCTGA AGACGAATTC 
CTGTCGTGTG ACTCTGGATC AGGAAGCGAA AGGGGAGATG GAGAGCGTGC AGGAGGAGAA 
GCAGAGCTCC TCATTCAAGA TGAATGTGTG GCAGTGAGAG GAACCGGAGG CATCTCGGAG 
AAAGAGAGGC TGAAGGAGAG AAGAGGAGAA GAGAGGACAC GTGAAATGGA TGAGGATGCA 
GATGTGGACA CAGCTGCACA AGACGGACAG GCTGAGAGAG AAACTGAAAC AGCGACACCA 
GCCACAGCTG TGCCGGCACC AGGAAACACA GAACCTGATA ATGAAGCTTC CATGCACTGT 
CCATCCTCTC GCCCCTGTAG CCCCGCTCAC AGTGTACAGG AGCTTCACTC CAGACTCTCT 
AGTAACCCAC CAAGATCCAG CCCTCTCAGA ACTGGACCCA CGTATACTTT CAAGAAAGCT 
CAGATGCTAC TGAGCACCAA GAAGAAGAAG CAGTCCCGTT TCCGTAGTGT TATTTCCGAT 
ATCTTTGATG GATCCATTCT GAGTCTTGTT CAGTGTTTGA CATGTGACCG GGTTTCGACA 
ACTGTGGAGA CGTTTCAGGA CCTTTCTTTG CCTATACCCG GTAAAGAAGA TTTGGCTAAG 
CTTCATTCCT CCATTCACCA GAGTGCACCT GTTAAAGCTG GTGTCTGCAC TGATGGCTAT 
GCTGCACAGG GCTGGATCTC CTACATCATG GACTCTATAC GAAGGTTTGT AGTGTCCTGT 
ATTCCAAGTT GGTTTTGGGG CCCCATGGTA ACGCTAGAGG ACTGTCTGGC AGCTTTCTTT 
GCTGCTGATG AGCTGAAAGG AGACAACATG TATAGCTGTG AACGGTGTAA AAAATTAAGA 
AATGGTGTGA AATATTGTAA AGTTCTGCGT CTACCAGAAA TATTGTGCAT TCACCTGAAG 
CGCTTCAGAC ATGAAGTCAT GTACTCGTTC AAAATAAATA GCCACGTGTC ATTCCCATTG 
GAGGGTTTGG ATCTCAAACC GTTCCTGGCT AAGGAAAGCC CGTCCCAGAT TACTACCTAT 
GACCTGCTGT CTGTCATCTG TCATCACGGC ACTGCTGGAA GTGGGCATTA CATAGCGTAC 
TGCCAGAATG TGATCAATGG ACAGTGGTAC GAGTTTGACG ATCAGTATGT AACAGAAGTG 
CACGAGACAG TAGTCCAGAA TGCTGAAGCA TATGTCCTAT TCTACAGGAA GAGCAGTGAA 
GAATCTGTGA GAGAAAGACA AAGGGTTGTC GCTCTGGCTA ATTTGAAGGA GCCCAGTTTG 
CTGCAGTTCT ACATCTCCAG AGAGTGGCTC AACAAATTCA ACACATTCAC TGAGCCCGGA 
CCCATCACCA ATCACACTTT CCTTTGTCAG CACGGAGGAA TTCCTCCTAC GAAGTACCAT 
TATGTGGATG ATCTGGTTGT GATCCTTCCT CAAAATGTGT GGGAATATCT CTACAACAGG 
TTTGGAGGTG GCCCGGCTGT GAATCACTTA TATGTGTGTG CAATTTGCCA AGTGGAGATC 
GAGACCTTAG CCAAACGACG GAAACTGGAG ATAGACACCT TCATTAAGTT AAATAAGGAG 
TTTCAGGCTG AAGAAGCTCC TACCGTCATC CTTTGTATAA GCATGCAGTG GTTCAGAGAA 
TGGGAGAACT TTGTCAAGGG CAAAGACAAT GAGCCACCAG GCCCCATTGA CAACAGCAAG 
ATTGCAGTAA TGAAAGGCGG TCATATCCAG CTTAAACAAG GAGCAGACTA TGGGCAGATA 
TCTGAAGAAA CGTGGCAGTA CTTGTTAAGT ATTTATGGCG GTGGGCCAGA GATTGCAGTG 
CGGCAAACCA TCAGCCCCCC AGACACTGAC ACACACGGGG AGAGGAAAAT AGAGGCAGAG 
ACCAGAGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299732.1
CDS	27..2861
Translation	MHVTLLMYCLDVPVLCFKQRKIVLTSESEKKIRMTDSGDLCPHLDSIGEVTKEELIQKSKGTCQSCGVGGPNLWACLQCDCPYVGCGESYSDHSTIHAQAKKHNLTVNLTTFRVWCYVCEREVFLEPKPVTPVSSAHRCKPHDQDPVSQTTCYPLKAVPIAVADEEGSESEEDELKPRGLTGMKNIGNSCYMNAALQALSNCPPLTQFFQDCSGLVRTDKKPALCKSYQKLISELWHKKRPSYVVPTTLFHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPLFDCSGGISEVEPDLSLDSCNLVDGDRSPSEDEFLSCDSGSGSERGDGERAGGEAELLIQDECVAVRGTGGISEKERLKERRGEERTREMDEDADVDTAAQDGQAERETETATPATAVPAPGNTEPDNEASMHCPSSRPCSPAHSVQELHSRLSSNPPRSSPLRTGPTYTFKKAQMLLSTKKKKQSRFRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSSIHQSAPVKAGVCTDGYAAQGWISYIMDSIRRFVVSCIPSWFWGPMVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLKPFLAKESPSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEAYVLFYRKSSEESVRERQRVVALANLKEPSLLQFYISREWLNKFNTFTEPGPITNHTFLCQHGGIPPTKYHYVDDLVVILPQNVWEYLYNRFGGGPAVNHLYVCAICQVEIETLAKRRKLEIDTFIKLNKEFQAEEAPTVILCISMQWFREWENFVKGKDNEPPGPIDNSKIAVMKGGHIQLKQGADYGQISEETWQYLLSIYGGGPEIAVRQTISPPDTDTHGERKIEAETRAL

Target ORF information:

RefSeq Version	XM_009301457.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301457.1

ORF Insert Sequence:

ATGCATGTCA CGCTCCTGAT GTATTGTTTG GATGTGCCTG TGCTTTGTTT CAAACAGAGG 
AAGATTGTGT TGACATCTGA GAGTGAGAAG AAAATCAGGA TGACTGACAG CGGGGATCTT 
TGTCCTCACT TGGACTCGAT TGGTGAAGTT ACTAAAGAAG AACTGATTCA GAAATCAAAG 
GGCACGTGTC AGTCATGTGG AGTAGGTGGA CCAAATCTCT GGGCATGTCT GCAGTGTGAC 
TGTCCTTATG TAGGCTGTGG AGAGTCGTAT TCAGACCACA GTACTATTCA TGCACAGGCC 
AAAAAGCACA ATCTGACAGT AAACCTGACC ACATTCAGGG TGTGGTGTTA TGTCTGCGAA 
AGAGAGGTGT TTCTGGAACC GAAGCCTGTT ACTCCTGTAT CATCAGCTCA CCGCTGCAAA 
CCACATGACC AGGATCCTGT TTCGCAGACT ACATGTTACC CATTGAAGGC GGTGCCTATC 
GCAGTGGCAG ATGAGGAGGG CTCAGAGTCT GAGGAGGATG AGCTCAAACC TAGAGGTCTG 
ACAGGGATGA AAAACATTGG AAACTCTTGT TATATGAATG CTGCACTGCA GGCCTTGTCC 
AACTGTCCTC CTTTGACTCA GTTTTTCCAG GATTGCAGTG GATTGGTGCG TACGGATAAG 
AAGCCGGCAT TGTGTAAAAG CTATCAGAAG CTTATCTCTG AACTCTGGCA CAAGAAACGG 
CCGAGTTATG TTGTGCCAAC TACTCTGTTT CATGGAATCA AACTGGTGAA CCCCATGTTT 
CGTGGCTATG CTCAACAGGA CACTCAGGAG TTTTTGCGAT GTCTGATGGA CCAGCTTCAC 
GAGGAGCTGA AGGAGCCTTT GTTTGACTGC AGCGGTGGCA TCTCTGAAGT CGAGCCTGAC 
CTCAGTCTGG ACAGCTGTAA TCTTGTGGAC GGAGACCGAA GCCCCTCTGA AGACGAATTC 
CTGTCGTGTG ACTCTGGATC AGGAAGCGAA AGGGGAGATG GAGAGCGTGC AGGAGGAGAA 
GCAGAGCTCC TCATTCAAGA TGAATGTGTG GCAGTGAGAG GAACCGGAGG CATCTCGGAG 
AAAGAGAGGC TGAAGGAGAG AAGAGGAGAA GAGAGGACAC GTGAAATGGA TGAGGATGCA 
GATGTGGACA CAGCTGCACA AGACGGACAG GCTGAGAGAG AAACTGAAAC AGCGACACCA 
GCCACAGCTG TGCCGGCACC AGGAAACACA GAACCTGATA ATGAAGCTTC CATGCACTGT 
CCATCCTCTC GCCCCTGTAG CCCCGCTCAC AGTGTACAGG AGCTTCACTC CAGACTCTCT 
AGTAACCCAC CAAGATCCAG CCCTCTCAGA ACTGGACCCA CGTATACTTT CAAGAAAGCT 
CAGATGCTAC TGAGCACCAA GAAGAAGAAG CAGTCCCGTT TCCGTAGTGT TATTTCCGAT 
ATCTTTGATG GATCCATTCT GAGTCTTGTT CAGTGTTTGA CATGTGACCG GGTTTCGACA 
ACTGTGGAGA CGTTTCAGGA CCTTTCTTTG CCTATACCCG GTAAAGAAGA TTTGGCTAAG 
CTTCATTCCT CCATTCACCA GAGTGCACCT GTTAAAGCTG GTGTCTGCAC TGATGGCTAT 
GCTGCACAGG GCTGGATCTC CTACATCATG GACTCTATAC GAAGGTTTGT AGTGTCCTGT 
ATTCCAAGTT GGTTTTGGGG CCCCATGGTA ACGCTAGAGG ACTGTCTGGC AGCTTTCTTT 
GCTGCTGATG AGCTGAAAGG AGACAACATG TATAGCTGTG AACGGTGTAA AAAATTAAGA 
AATGGTGTGA AATATTGTAA AGTTCTGCGT CTACCAGAAA TATTGTGCAT TCACCTGAAG 
CGCTTCAGAC ATGAAGTCAT GTACTCGTTC AAAATAAATA GCCACGTGTC ATTCCCATTG 
GAGGGTTTGG ATCTCAAACC GTTCCTGGCT AAGGAAAGCC CGTCCCAGAT TACTACCTAT 
GACCTGCTGT CTGTCATCTG TCATCACGGC ACTGCTGGAA GTGGGCATTA CATAGCGTAC 
TGCCAGAATG TGATCAATGG ACAGTGGTAC GAGTTTGACG ATCAGTATGT AACAGAAGTG 
CACGAGACAG TAGTCCAGAA TGCTGAAGCA TATGTCCTAT TCTACAGGAA GAGCAGTGAA 
GAATCTGTGA GAGAAAGACA AAGGGTTGTC GCTCTGGCTA ATTTGAAGGA GCCCAGTTTG 
CTGCAGTTCT ACATCTCCAG AGAGTGGCTC AACAAATTCA ACACATTCAC TGAGCCCGGA 
CCCATCACCA ATCACACTTT CCTTTGTCAG CACGGAGGAA TTCCTCCTAC GAAGTACCAT 
TATGTGGATG ATCTGGTTGT GATCCTTCCT CAAAATGTGT GGGAATATCT CTACAACAGG 
TTTGGAGGTG GCCCGGCTGT GAATCACTTA TATGTGTGTG CAATTTGCCA AGTGGAGATC 
GAGACCTTAG CCAAACGACG GAAACTGGAG ATAGACACCT TCATTAAGTT AAATAAGGAG 
TTTCAGGCTG AAGAAGCTCC TACCGTCATC CTTTGTATAA GCATGCAGTG GTTCAGAGAA 
TGGGAGAACT TTGTCAAGGG CAAAGACAAT GAGCCACCAG GCCCCATTGA CAACAGCAAG 
ATTGCAGTAA TGAAAGGCGG TCATATCCAG CTTAAACAAG GAGCAGACTA TGGGCAGATA 
TCTGAAGAAA CGTGGCAGTA CTTGTTAAGT ATTTATGGCG GTGGGCCAGA GATTGCAGTG 
CGGCAAACCA TCAGCCCCCC AGACACTGAC ACACACGGGG AGAGGAAAAT AGAGGCAGAG 
ACCAGAGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp20 ( XM_009301457.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299732.1 Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10428
Clone ID Related Accession (Same CDS sequence)	XM_009301457.1 , XM_009301457.2
Accession Version	XM_009301457.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGTCA CGCTCCTGAT GTATTGTTTG GATGTGCCTG TGCTTTGTTT CAAACAGAGG 
AAGATTGTGT TGACATCTGA GAGTGAGAAG AAAATCAGGA TGACTGACAG CGGGGATCTT 
TGTCCTCACT TGGACTCGAT TGGTGAAGTT ACTAAAGAAG AACTGATTCA GAAATCAAAG 
GGCACGTGTC AGTCATGTGG AGTAGGTGGA CCAAATCTCT GGGCATGTCT GCAGTGTGAC 
TGTCCTTATG TAGGCTGTGG AGAGTCGTAT TCAGACCACA GTACTATTCA TGCACAGGCC 
AAAAAGCACA ATCTGACAGT AAACCTGACC ACATTCAGGG TGTGGTGTTA TGTCTGCGAA 
AGAGAGGTGT TTCTGGAACC GAAGCCTGTT ACTCCTGTAT CATCAGCTCA CCGCTGCAAA 
CCACATGACC AGGATCCTGT TTCGCAGACT ACATGTTACC CATTGAAGGC GGTGCCTATC 
GCAGTGGCAG ATGAGGAGGG CTCAGAGTCT GAGGAGGATG AGCTCAAACC TAGAGGTCTG 
ACAGGGATGA AAAACATTGG AAACTCTTGT TATATGAATG CTGCACTGCA GGCCTTGTCC 
AACTGTCCTC CTTTGACTCA GTTTTTCCAG GATTGCAGTG GATTGGTGCG TACGGATAAG 
AAGCCGGCAT TGTGTAAAAG CTATCAGAAG CTTATCTCTG AACTCTGGCA CAAGAAACGG 
CCGAGTTATG TTGTGCCAAC TACTCTGTTT CATGGAATCA AACTGGTGAA CCCCATGTTT 
CGTGGCTATG CTCAACAGGA CACTCAGGAG TTTTTGCGAT GTCTGATGGA CCAGCTTCAC 
GAGGAGCTGA AGGAGCCTTT GTTTGACTGC AGCGGTGGCA TCTCTGAAGT CGAGCCTGAC 
CTCAGTCTGG ACAGCTGTAA TCTTGTGGAC GGAGACCGAA GCCCCTCTGA AGACGAATTC 
CTGTCGTGTG ACTCTGGATC AGGAAGCGAA AGGGGAGATG GAGAGCGTGC AGGAGGAGAA 
GCAGAGCTCC TCATTCAAGA TGAATGTGTG GCAGTGAGAG GAACCGGAGG CATCTCGGAG 
AAAGAGAGGC TGAAGGAGAG AAGAGGAGAA GAGAGGACAC GTGAAATGGA TGAGGATGCA 
GATGTGGACA CAGCTGCACA AGACGGACAG GCTGAGAGAG AAACTGAAAC AGCGACACCA 
GCCACAGCTG TGCCGGCACC AGGAAACACA GAACCTGATA ATGAAGCTTC CATGCACTGT 
CCATCCTCTC GCCCCTGTAG CCCCGCTCAC AGTGTACAGG AGCTTCACTC CAGACTCTCT 
AGTAACCCAC CAAGATCCAG CCCTCTCAGA ACTGGACCCA CGTATACTTT CAAGAAAGCT 
CAGATGCTAC TGAGCACCAA GAAGAAGAAG CAGTCCCGTT TCCGTAGTGT TATTTCCGAT 
ATCTTTGATG GATCCATTCT GAGTCTTGTT CAGTGTTTGA CATGTGACCG GGTTTCGACA 
ACTGTGGAGA CGTTTCAGGA CCTTTCTTTG CCTATACCCG GTAAAGAAGA TTTGGCTAAG 
CTTCATTCCT CCATTCACCA GAGTGCACCT GTTAAAGCTG GTGTCTGCAC TGATGGCTAT 
GCTGCACAGG GCTGGATCTC CTACATCATG GACTCTATAC GAAGGTTTGT AGTGTCCTGT 
ATTCCAAGTT GGTTTTGGGG CCCCATGGTA ACGCTAGAGG ACTGTCTGGC AGCTTTCTTT 
GCTGCTGATG AGCTGAAAGG AGACAACATG TATAGCTGTG AACGGTGTAA AAAATTAAGA 
AATGGTGTGA AATATTGTAA AGTTCTGCGT CTACCAGAAA TATTGTGCAT TCACCTGAAG 
CGCTTCAGAC ATGAAGTCAT GTACTCGTTC AAAATAAATA GCCACGTGTC ATTCCCATTG 
GAGGGTTTGG ATCTCAAACC GTTCCTGGCT AAGGAAAGCC CGTCCCAGAT TACTACCTAT 
GACCTGCTGT CTGTCATCTG TCATCACGGC ACTGCTGGAA GTGGGCATTA CATAGCGTAC 
TGCCAGAATG TGATCAATGG ACAGTGGTAC GAGTTTGACG ATCAGTATGT AACAGAAGTG 
CACGAGACAG TAGTCCAGAA TGCTGAAGCA TATGTCCTAT TCTACAGGAA GAGCAGTGAA 
GAATCTGTGA GAGAAAGACA AAGGGTTGTC GCTCTGGCTA ATTTGAAGGA GCCCAGTTTG 
CTGCAGTTCT ACATCTCCAG AGAGTGGCTC AACAAATTCA ACACATTCAC TGAGCCCGGA 
CCCATCACCA ATCACACTTT CCTTTGTCAG CACGGAGGAA TTCCTCCTAC GAAGTACCAT 
TATGTGGATG ATCTGGTTGT GATCCTTCCT CAAAATGTGT GGGAATATCT CTACAACAGG 
TTTGGAGGTG GCCCGGCTGT GAATCACTTA TATGTGTGTG CAATTTGCCA AGTGGAGATC 
GAGACCTTAG CCAAACGACG GAAACTGGAG ATAGACACCT TCATTAAGTT AAATAAGGAG 
TTTCAGGCTG AAGAAGCTCC TACCGTCATC CTTTGTATAA GCATGCAGTG GTTCAGAGAA 
TGGGAGAACT TTGTCAAGGG CAAAGACAAT GAGCCACCAG GCCCCATTGA CAACAGCAAG 
ATTGCAGTAA TGAAAGGCGG TCATATCCAG CTTAAACAAG GAGCAGACTA TGGGCAGATA 
TCTGAAGAAA CGTGGCAGTA CTTGTTAAGT ATTTATGGCG GTGGGCCAGA GATTGCAGTG 
CGGCAAACCA TCAGCCCCCC AGACACTGAC ACACACGGGG AGAGGAAAAT AGAGGCAGAG 
ACCAGAGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299732.1
CDS	28..2862
Translation	MHVTLLMYCLDVPVLCFKQRKIVLTSESEKKIRMTDSGDLCPHLDSIGEVTKEELIQKSKGTCQSCGVGGPNLWACLQCDCPYVGCGESYSDHSTIHAQAKKHNLTVNLTTFRVWCYVCEREVFLEPKPVTPVSSAHRCKPHDQDPVSQTTCYPLKAVPIAVADEEGSESEEDELKPRGLTGMKNIGNSCYMNAALQALSNCPPLTQFFQDCSGLVRTDKKPALCKSYQKLISELWHKKRPSYVVPTTLFHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPLFDCSGGISEVEPDLSLDSCNLVDGDRSPSEDEFLSCDSGSGSERGDGERAGGEAELLIQDECVAVRGTGGISEKERLKERRGEERTREMDEDADVDTAAQDGQAERETETATPATAVPAPGNTEPDNEASMHCPSSRPCSPAHSVQELHSRLSSNPPRSSPLRTGPTYTFKKAQMLLSTKKKKQSRFRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSSIHQSAPVKAGVCTDGYAAQGWISYIMDSIRRFVVSCIPSWFWGPMVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLKPFLAKESPSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEAYVLFYRKSSEESVRERQRVVALANLKEPSLLQFYISREWLNKFNTFTEPGPITNHTFLCQHGGIPPTKYHYVDDLVVILPQNVWEYLYNRFGGGPAVNHLYVCAICQVEIETLAKRRKLEIDTFIKLNKEFQAEEAPTVILCISMQWFREWENFVKGKDNEPPGPIDNSKIAVMKGGHIQLKQGADYGQISEETWQYLLSIYGGGPEIAVRQTISPPDTDTHGERKIEAETRAL

Target ORF information:

RefSeq Version	XM_009301457.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301457.2

ORF Insert Sequence:

ATGCATGTCA CGCTCCTGAT GTATTGTTTG GATGTGCCTG TGCTTTGTTT CAAACAGAGG 
AAGATTGTGT TGACATCTGA GAGTGAGAAG AAAATCAGGA TGACTGACAG CGGGGATCTT 
TGTCCTCACT TGGACTCGAT TGGTGAAGTT ACTAAAGAAG AACTGATTCA GAAATCAAAG 
GGCACGTGTC AGTCATGTGG AGTAGGTGGA CCAAATCTCT GGGCATGTCT GCAGTGTGAC 
TGTCCTTATG TAGGCTGTGG AGAGTCGTAT TCAGACCACA GTACTATTCA TGCACAGGCC 
AAAAAGCACA ATCTGACAGT AAACCTGACC ACATTCAGGG TGTGGTGTTA TGTCTGCGAA 
AGAGAGGTGT TTCTGGAACC GAAGCCTGTT ACTCCTGTAT CATCAGCTCA CCGCTGCAAA 
CCACATGACC AGGATCCTGT TTCGCAGACT ACATGTTACC CATTGAAGGC GGTGCCTATC 
GCAGTGGCAG ATGAGGAGGG CTCAGAGTCT GAGGAGGATG AGCTCAAACC TAGAGGTCTG 
ACAGGGATGA AAAACATTGG AAACTCTTGT TATATGAATG CTGCACTGCA GGCCTTGTCC 
AACTGTCCTC CTTTGACTCA GTTTTTCCAG GATTGCAGTG GATTGGTGCG TACGGATAAG 
AAGCCGGCAT TGTGTAAAAG CTATCAGAAG CTTATCTCTG AACTCTGGCA CAAGAAACGG 
CCGAGTTATG TTGTGCCAAC TACTCTGTTT CATGGAATCA AACTGGTGAA CCCCATGTTT 
CGTGGCTATG CTCAACAGGA CACTCAGGAG TTTTTGCGAT GTCTGATGGA CCAGCTTCAC 
GAGGAGCTGA AGGAGCCTTT GTTTGACTGC AGCGGTGGCA TCTCTGAAGT CGAGCCTGAC 
CTCAGTCTGG ACAGCTGTAA TCTTGTGGAC GGAGACCGAA GCCCCTCTGA AGACGAATTC 
CTGTCGTGTG ACTCTGGATC AGGAAGCGAA AGGGGAGATG GAGAGCGTGC AGGAGGAGAA 
GCAGAGCTCC TCATTCAAGA TGAATGTGTG GCAGTGAGAG GAACCGGAGG CATCTCGGAG 
AAAGAGAGGC TGAAGGAGAG AAGAGGAGAA GAGAGGACAC GTGAAATGGA TGAGGATGCA 
GATGTGGACA CAGCTGCACA AGACGGACAG GCTGAGAGAG AAACTGAAAC AGCGACACCA 
GCCACAGCTG TGCCGGCACC AGGAAACACA GAACCTGATA ATGAAGCTTC CATGCACTGT 
CCATCCTCTC GCCCCTGTAG CCCCGCTCAC AGTGTACAGG AGCTTCACTC CAGACTCTCT 
AGTAACCCAC CAAGATCCAG CCCTCTCAGA ACTGGACCCA CGTATACTTT CAAGAAAGCT 
CAGATGCTAC TGAGCACCAA GAAGAAGAAG CAGTCCCGTT TCCGTAGTGT TATTTCCGAT 
ATCTTTGATG GATCCATTCT GAGTCTTGTT CAGTGTTTGA CATGTGACCG GGTTTCGACA 
ACTGTGGAGA CGTTTCAGGA CCTTTCTTTG CCTATACCCG GTAAAGAAGA TTTGGCTAAG 
CTTCATTCCT CCATTCACCA GAGTGCACCT GTTAAAGCTG GTGTCTGCAC TGATGGCTAT 
GCTGCACAGG GCTGGATCTC CTACATCATG GACTCTATAC GAAGGTTTGT AGTGTCCTGT 
ATTCCAAGTT GGTTTTGGGG CCCCATGGTA ACGCTAGAGG ACTGTCTGGC AGCTTTCTTT 
GCTGCTGATG AGCTGAAAGG AGACAACATG TATAGCTGTG AACGGTGTAA AAAATTAAGA 
AATGGTGTGA AATATTGTAA AGTTCTGCGT CTACCAGAAA TATTGTGCAT TCACCTGAAG 
CGCTTCAGAC ATGAAGTCAT GTACTCGTTC AAAATAAATA GCCACGTGTC ATTCCCATTG 
GAGGGTTTGG ATCTCAAACC GTTCCTGGCT AAGGAAAGCC CGTCCCAGAT TACTACCTAT 
GACCTGCTGT CTGTCATCTG TCATCACGGC ACTGCTGGAA GTGGGCATTA CATAGCGTAC 
TGCCAGAATG TGATCAATGG ACAGTGGTAC GAGTTTGACG ATCAGTATGT AACAGAAGTG 
CACGAGACAG TAGTCCAGAA TGCTGAAGCA TATGTCCTAT TCTACAGGAA GAGCAGTGAA 
GAATCTGTGA GAGAAAGACA AAGGGTTGTC GCTCTGGCTA ATTTGAAGGA GCCCAGTTTG 
CTGCAGTTCT ACATCTCCAG AGAGTGGCTC AACAAATTCA ACACATTCAC TGAGCCCGGA 
CCCATCACCA ATCACACTTT CCTTTGTCAG CACGGAGGAA TTCCTCCTAC GAAGTACCAT 
TATGTGGATG ATCTGGTTGT GATCCTTCCT CAAAATGTGT GGGAATATCT CTACAACAGG 
TTTGGAGGTG GCCCGGCTGT GAATCACTTA TATGTGTGTG CAATTTGCCA AGTGGAGATC 
GAGACCTTAG CCAAACGACG GAAACTGGAG ATAGACACCT TCATTAAGTT AAATAAGGAG 
TTTCAGGCTG AAGAAGCTCC TACCGTCATC CTTTGTATAA GCATGCAGTG GTTCAGAGAA 
TGGGAGAACT TTGTCAAGGG CAAAGACAAT GAGCCACCAG GCCCCATTGA CAACAGCAAG 
ATTGCAGTAA TGAAAGGCGG TCATATCCAG CTTAAACAAG GAGCAGACTA TGGGCAGATA 
TCTGAAGAAA CGTGGCAGTA CTTGTTAAGT ATTTATGGCG GTGGGCCAGA GATTGCAGTG 
CGGCAAACCA TCAGCCCCCC AGACACTGAC ACACACGGGG AGAGGAAAAT AGAGGCAGAG 
ACCAGAGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp20 ( XM_009301458.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299733.1 Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40597
Clone ID Related Accession (Same CDS sequence)	XM_009301458.2 , XM_009301458.3
Accession Version	XM_009301458.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGACA GCGGGGATCT TTGTCCTCAC TTGGACTCGA TTGGTGAAGT TACTAAAGAA 
GAACTGATTC AGAAATCAAA GGGCACGTGT CAGTCATGTG GAGTAGGTGG ACCAAATCTC 
TGGGCATGTC TGCAGTGTGA CTGTCCTTAT GTAGGCTGTG GAGAGTCGTA TTCAGACCAC 
AGTACTATTC ATGCACAGGC CAAAAAGCAC AATCTGACAG TAAACCTGAC CACATTCAGG 
GTGTGGTGTT ATGTCTGCGA AAGAGAGGTG TTTCTGGAAC CGAAGCCTGT TACTCCTGTA 
TCATCAGCTC ACCGCTGCAA ACCACATGAC CAGGATCCTG TTTCGCAGAC TACATGTTAC 
CCATTGAAGG CGGTGCCTAT CGCAGTGGCA GATGAGGAGG GCTCAGAGTC TGAGGAGGAT 
GAGCTCAAAC CTAGAGGTCT GACAGGGATG AAAAACATTG GAAACTCTTG TTATATGAAT 
GCTGCACTGC AGGCCTTGTC CAACTGTCCT CCTTTGACTC AGTTTTTCCA GGATTGCAGT 
GGATTGGTGC GTACGGATAA GAAGCCGGCA TTGTGTAAAA GCTATCAGAA GCTTATCTCT 
GAACTCTGGC ACAAGAAACG GCCGAGTTAT GTTGTGCCAA CTACTCTGTT TCATGGAATC 
AAACTGGTGA ACCCCATGTT TCGTGGCTAT GCTCAACAGG ACACTCAGGA GTTTTTGCGA 
TGTCTGATGG ACCAGCTTCA CGAGGAGCTG AAGGAGCCTT TGTTTGACTG CAGCGGTGGC 
ATCTCTGAAG TCGAGCCTGA CCTCAGTCTG GACAGCTGTA ATCTTGTGGA CGGAGACCGA 
AGCCCCTCTG AAGACGAATT CCTGTCGTGT GACTCTGGAT CAGGAAGCGA AAGGGGAGAT 
GGAGAGCGTG CAGGAGGAGA AGCAGAGCTC CTCATTCAAG ATGAATGTGT GGCAGTGAGA 
GGAACCGGAG GCATCTCGGA GAAAGAGAGG CTGAAGGAGA GAAGAGGAGA AGAGAGGACA 
CGTGAAATGG ATGAGGATGC AGATGTGGAC ACAGCTGCAC AAGACGGACA GGCTGAGAGA 
GAAACTGAAA CAGCGACACC AGCCACAGCT GTGCCGGCAC CAGGAAACAC AGAACCTGAT 
AATGAAGCTT CCATGCACTG TCCATCCTCT CGCCCCTGTA GCCCCGCTCA CAGTGTACAG 
GAGCTTCACT CCAGACTCTC TAGTAACCCA CCAAGATCCA GCCCTCTCAG AACTGGACCC 
ACGTATACTT TCAAGAAAGC TCAGATGCTA CTGAGCACCA AGAAGAAGAA GCAGTCCCGT 
TTCCGTAGTG TTATTTCCGA TATCTTTGAT GGATCCATTC TGAGTCTTGT TCAGTGTTTG 
ACATGTGACC GGGTTTCGAC AACTGTGGAG ACGTTTCAGG ACCTTTCTTT GCCTATACCC 
GGTAAAGAAG ATTTGGCTAA GCTTCATTCC TCCATTCACC AGAGTGCACC TGTTAAAGCT 
GGTGTCTGCA CTGATGGCTA TGCTGCACAG GGCTGGATCT CCTACATCAT GGACTCTATA 
CGAAGGTTTG TAGTGTCCTG TATTCCAAGT TGGTTTTGGG GCCCCATGGT AACGCTAGAG 
GACTGTCTGG CAGCTTTCTT TGCTGCTGAT GAGCTGAAAG GAGACAACAT GTATAGCTGT 
GAACGGTGTA AAAAATTAAG AAATGGTGTG AAATATTGTA AAGTTCTGCG TCTACCAGAA 
ATATTGTGCA TTCACCTGAA GCGCTTCAGA CATGAAGTCA TGTACTCGTT CAAAATAAAT 
AGCCACGTGT CATTCCCATT GGAGGGTTTG GATCTCAAAC CGTTCCTGGC TAAGGAAAGC 
CCGTCCCAGA TTACTACCTA TGACCTGCTG TCTGTCATCT GTCATCACGG CACTGCTGGA 
AGTGGGCATT ACATAGCGTA CTGCCAGAAT GTGATCAATG GACAGTGGTA CGAGTTTGAC 
GATCAGTATG TAACAGAAGT GCACGAGACA GTAGTCCAGA ATGCTGAAGC ATATGTCCTA 
TTCTACAGGA AGAGCAGTGA AGAATCTGTG AGAGAAAGAC AAAGGGTTGT CGCTCTGGCT 
AATTTGAAGG AGCCCAGTTT GCTGCAGTTC TACATCTCCA GAGAGTGGCT CAACAAATTC 
AACACATTCA CTGAGCCCGG ACCCATCACC AATCACACTT TCCTTTGTCA GCACGGAGGA 
ATTCCTCCTA CGAAGTACCA TTATGTGGAT GATCTGGTTG TGATCCTTCC TCAAAATGTG 
TGGGAATATC TCTACAACAG GTTTGGAGGT GGCCCGGCTG TGAATCACTT ATATGTGTGT 
GCAATTTGCC AAGTGGAGAT CGAGACCTTA GCCAAACGAC GGAAACTGGA GATAGACACC 
TTCATTAAGT TAAATAAGGA GTTTCAGGCT GAAGAAGCTC CTACCGTCAT CCTTTGTATA 
AGCATGCAGT GGTTCAGAGA ATGGGAGAAC TTTGTCAAGG GCAAAGACAA TGAGCCACCA 
GGCCCCATTG ACAACAGCAA GATTGCAGTA ATGAAAGGCG GTCATATCCA GCTTAAACAA 
GGAGCAGACT ATGGGCAGAT ATCTGAAGAA ACGTGGCAGT ACTTGTTAAG TATTTATGGC 
GGTGGGCCAG AGATTGCAGT GCGGCAAACC ATCAGCCCCC CAGACACTGA CACACACGGG 
GAGAGGAAAA TAGAGGCAGA GACCAGAGCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299733.1
CDS	155..2890
Translation	MTDSGDLCPHLDSIGEVTKEELIQKSKGTCQSCGVGGPNLWACLQCDCPYVGCGESYSDHSTIHAQAKKHNLTVNLTTFRVWCYVCEREVFLEPKPVTPVSSAHRCKPHDQDPVSQTTCYPLKAVPIAVADEEGSESEEDELKPRGLTGMKNIGNSCYMNAALQALSNCPPLTQFFQDCSGLVRTDKKPALCKSYQKLISELWHKKRPSYVVPTTLFHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPLFDCSGGISEVEPDLSLDSCNLVDGDRSPSEDEFLSCDSGSGSERGDGERAGGEAELLIQDECVAVRGTGGISEKERLKERRGEERTREMDEDADVDTAAQDGQAERETETATPATAVPAPGNTEPDNEASMHCPSSRPCSPAHSVQELHSRLSSNPPRSSPLRTGPTYTFKKAQMLLSTKKKKQSRFRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSSIHQSAPVKAGVCTDGYAAQGWISYIMDSIRRFVVSCIPSWFWGPMVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLKPFLAKESPSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEAYVLFYRKSSEESVRERQRVVALANLKEPSLLQFYISREWLNKFNTFTEPGPITNHTFLCQHGGIPPTKYHYVDDLVVILPQNVWEYLYNRFGGGPAVNHLYVCAICQVEIETLAKRRKLEIDTFIKLNKEFQAEEAPTVILCISMQWFREWENFVKGKDNEPPGPIDNSKIAVMKGGHIQLKQGADYGQISEETWQYLLSIYGGGPEIAVRQTISPPDTDTHGERKIEAETRAL

Target ORF information:

RefSeq Version	XM_009301458.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301458.2

ORF Insert Sequence:

ATGACTGACA GCGGGGATCT TTGTCCTCAC TTGGACTCGA TTGGTGAAGT TACTAAAGAA 
GAACTGATTC AGAAATCAAA GGGCACGTGT CAGTCATGTG GAGTAGGTGG ACCAAATCTC 
TGGGCATGTC TGCAGTGTGA CTGTCCTTAT GTAGGCTGTG GAGAGTCGTA TTCAGACCAC 
AGTACTATTC ATGCACAGGC CAAAAAGCAC AATCTGACAG TAAACCTGAC CACATTCAGG 
GTGTGGTGTT ATGTCTGCGA AAGAGAGGTG TTTCTGGAAC CGAAGCCTGT TACTCCTGTA 
TCATCAGCTC ACCGCTGCAA ACCACATGAC CAGGATCCTG TTTCGCAGAC TACATGTTAC 
CCATTGAAGG CGGTGCCTAT CGCAGTGGCA GATGAGGAGG GCTCAGAGTC TGAGGAGGAT 
GAGCTCAAAC CTAGAGGTCT GACAGGGATG AAAAACATTG GAAACTCTTG TTATATGAAT 
GCTGCACTGC AGGCCTTGTC CAACTGTCCT CCTTTGACTC AGTTTTTCCA GGATTGCAGT 
GGATTGGTGC GTACGGATAA GAAGCCGGCA TTGTGTAAAA GCTATCAGAA GCTTATCTCT 
GAACTCTGGC ACAAGAAACG GCCGAGTTAT GTTGTGCCAA CTACTCTGTT TCATGGAATC 
AAACTGGTGA ACCCCATGTT TCGTGGCTAT GCTCAACAGG ACACTCAGGA GTTTTTGCGA 
TGTCTGATGG ACCAGCTTCA CGAGGAGCTG AAGGAGCCTT TGTTTGACTG CAGCGGTGGC 
ATCTCTGAAG TCGAGCCTGA CCTCAGTCTG GACAGCTGTA ATCTTGTGGA CGGAGACCGA 
AGCCCCTCTG AAGACGAATT CCTGTCGTGT GACTCTGGAT CAGGAAGCGA AAGGGGAGAT 
GGAGAGCGTG CAGGAGGAGA AGCAGAGCTC CTCATTCAAG ATGAATGTGT GGCAGTGAGA 
GGAACCGGAG GCATCTCGGA GAAAGAGAGG CTGAAGGAGA GAAGAGGAGA AGAGAGGACA 
CGTGAAATGG ATGAGGATGC AGATGTGGAC ACAGCTGCAC AAGACGGACA GGCTGAGAGA 
GAAACTGAAA CAGCGACACC AGCCACAGCT GTGCCGGCAC CAGGAAACAC AGAACCTGAT 
AATGAAGCTT CCATGCACTG TCCATCCTCT CGCCCCTGTA GCCCCGCTCA CAGTGTACAG 
GAGCTTCACT CCAGACTCTC TAGTAACCCA CCAAGATCCA GCCCTCTCAG AACTGGACCC 
ACGTATACTT TCAAGAAAGC TCAGATGCTA CTGAGCACCA AGAAGAAGAA GCAGTCCCGT 
TTCCGTAGTG TTATTTCCGA TATCTTTGAT GGATCCATTC TGAGTCTTGT TCAGTGTTTG 
ACATGTGACC GGGTTTCGAC AACTGTGGAG ACGTTTCAGG ACCTTTCTTT GCCTATACCC 
GGTAAAGAAG ATTTGGCTAA GCTTCATTCC TCCATTCACC AGAGTGCACC TGTTAAAGCT 
GGTGTCTGCA CTGATGGCTA TGCTGCACAG GGCTGGATCT CCTACATCAT GGACTCTATA 
CGAAGGTTTG TAGTGTCCTG TATTCCAAGT TGGTTTTGGG GCCCCATGGT AACGCTAGAG 
GACTGTCTGG CAGCTTTCTT TGCTGCTGAT GAGCTGAAAG GAGACAACAT GTATAGCTGT 
GAACGGTGTA AAAAATTAAG AAATGGTGTG AAATATTGTA AAGTTCTGCG TCTACCAGAA 
ATATTGTGCA TTCACCTGAA GCGCTTCAGA CATGAAGTCA TGTACTCGTT CAAAATAAAT 
AGCCACGTGT CATTCCCATT GGAGGGTTTG GATCTCAAAC CGTTCCTGGC TAAGGAAAGC 
CCGTCCCAGA TTACTACCTA TGACCTGCTG TCTGTCATCT GTCATCACGG CACTGCTGGA 
AGTGGGCATT ACATAGCGTA CTGCCAGAAT GTGATCAATG GACAGTGGTA CGAGTTTGAC 
GATCAGTATG TAACAGAAGT GCACGAGACA GTAGTCCAGA ATGCTGAAGC ATATGTCCTA 
TTCTACAGGA AGAGCAGTGA AGAATCTGTG AGAGAAAGAC AAAGGGTTGT CGCTCTGGCT 
AATTTGAAGG AGCCCAGTTT GCTGCAGTTC TACATCTCCA GAGAGTGGCT CAACAAATTC 
AACACATTCA CTGAGCCCGG ACCCATCACC AATCACACTT TCCTTTGTCA GCACGGAGGA 
ATTCCTCCTA CGAAGTACCA TTATGTGGAT GATCTGGTTG TGATCCTTCC TCAAAATGTG 
TGGGAATATC TCTACAACAG GTTTGGAGGT GGCCCGGCTG TGAATCACTT ATATGTGTGT 
GCAATTTGCC AAGTGGAGAT CGAGACCTTA GCCAAACGAC GGAAACTGGA GATAGACACC 
TTCATTAAGT TAAATAAGGA GTTTCAGGCT GAAGAAGCTC CTACCGTCAT CCTTTGTATA 
AGCATGCAGT GGTTCAGAGA ATGGGAGAAC TTTGTCAAGG GCAAAGACAA TGAGCCACCA 
GGCCCCATTG ACAACAGCAA GATTGCAGTA ATGAAAGGCG GTCATATCCA GCTTAAACAA 
GGAGCAGACT ATGGGCAGAT ATCTGAAGAA ACGTGGCAGT ACTTGTTAAG TATTTATGGC 
GGTGGGCCAG AGATTGCAGT GCGGCAAACC ATCAGCCCCC CAGACACTGA CACACACGGG 
GAGAGGAAAA TAGAGGCAGA GACCAGAGCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp20 ( XM_009301458.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299733.1 Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X2, mRNA.

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CloneID	ODa40597
Clone ID Related Accession (Same CDS sequence)	XM_009301458.2 , XM_009301458.3
Accession Version	XM_009301458.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301458.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGACA GCGGGGATCT TTGTCCTCAC TTGGACTCGA TTGGTGAAGT TACTAAAGAA 
GAACTGATTC AGAAATCAAA GGGCACGTGT CAGTCATGTG GAGTAGGTGG ACCAAATCTC 
TGGGCATGTC TGCAGTGTGA CTGTCCTTAT GTAGGCTGTG GAGAGTCGTA TTCAGACCAC 
AGTACTATTC ATGCACAGGC CAAAAAGCAC AATCTGACAG TAAACCTGAC CACATTCAGG 
GTGTGGTGTT ATGTCTGCGA AAGAGAGGTG TTTCTGGAAC CGAAGCCTGT TACTCCTGTA 
TCATCAGCTC ACCGCTGCAA ACCACATGAC CAGGATCCTG TTTCGCAGAC TACATGTTAC 
CCATTGAAGG CGGTGCCTAT CGCAGTGGCA GATGAGGAGG GCTCAGAGTC TGAGGAGGAT 
GAGCTCAAAC CTAGAGGTCT GACAGGGATG AAAAACATTG GAAACTCTTG TTATATGAAT 
GCTGCACTGC AGGCCTTGTC CAACTGTCCT CCTTTGACTC AGTTTTTCCA GGATTGCAGT 
GGATTGGTGC GTACGGATAA GAAGCCGGCA TTGTGTAAAA GCTATCAGAA GCTTATCTCT 
GAACTCTGGC ACAAGAAACG GCCGAGTTAT GTTGTGCCAA CTACTCTGTT TCATGGAATC 
AAACTGGTGA ACCCCATGTT TCGTGGCTAT GCTCAACAGG ACACTCAGGA GTTTTTGCGA 
TGTCTGATGG ACCAGCTTCA CGAGGAGCTG AAGGAGCCTT TGTTTGACTG CAGCGGTGGC 
ATCTCTGAAG TCGAGCCTGA CCTCAGTCTG GACAGCTGTA ATCTTGTGGA CGGAGACCGA 
AGCCCCTCTG AAGACGAATT CCTGTCGTGT GACTCTGGAT CAGGAAGCGA AAGGGGAGAT 
GGAGAGCGTG CAGGAGGAGA AGCAGAGCTC CTCATTCAAG ATGAATGTGT GGCAGTGAGA 
GGAACCGGAG GCATCTCGGA GAAAGAGAGG CTGAAGGAGA GAAGAGGAGA AGAGAGGACA 
CGTGAAATGG ATGAGGATGC AGATGTGGAC ACAGCTGCAC AAGACGGACA GGCTGAGAGA 
GAAACTGAAA CAGCGACACC AGCCACAGCT GTGCCGGCAC CAGGAAACAC AGAACCTGAT 
AATGAAGCTT CCATGCACTG TCCATCCTCT CGCCCCTGTA GCCCCGCTCA CAGTGTACAG 
GAGCTTCACT CCAGACTCTC TAGTAACCCA CCAAGATCCA GCCCTCTCAG AACTGGACCC 
ACGTATACTT TCAAGAAAGC TCAGATGCTA CTGAGCACCA AGAAGAAGAA GCAGTCCCGT 
TTCCGTAGTG TTATTTCCGA TATCTTTGAT GGATCCATTC TGAGTCTTGT TCAGTGTTTG 
ACATGTGACC GGGTTTCGAC AACTGTGGAG ACGTTTCAGG ACCTTTCTTT GCCTATACCC 
GGTAAAGAAG ATTTGGCTAA GCTTCATTCC TCCATTCACC AGAGTGCACC TGTTAAAGCT 
GGTGTCTGCA CTGATGGCTA TGCTGCACAG GGCTGGATCT CCTACATCAT GGACTCTATA 
CGAAGGTTTG TAGTGTCCTG TATTCCAAGT TGGTTTTGGG GCCCCATGGT AACGCTAGAG 
GACTGTCTGG CAGCTTTCTT TGCTGCTGAT GAGCTGAAAG GAGACAACAT GTATAGCTGT 
GAACGGTGTA AAAAATTAAG AAATGGTGTG AAATATTGTA AAGTTCTGCG TCTACCAGAA 
ATATTGTGCA TTCACCTGAA GCGCTTCAGA CATGAAGTCA TGTACTCGTT CAAAATAAAT 
AGCCACGTGT CATTCCCATT GGAGGGTTTG GATCTCAAAC CGTTCCTGGC TAAGGAAAGC 
CCGTCCCAGA TTACTACCTA TGACCTGCTG TCTGTCATCT GTCATCACGG CACTGCTGGA 
AGTGGGCATT ACATAGCGTA CTGCCAGAAT GTGATCAATG GACAGTGGTA CGAGTTTGAC 
GATCAGTATG TAACAGAAGT GCACGAGACA GTAGTCCAGA ATGCTGAAGC ATATGTCCTA 
TTCTACAGGA AGAGCAGTGA AGAATCTGTG AGAGAAAGAC AAAGGGTTGT CGCTCTGGCT 
AATTTGAAGG AGCCCAGTTT GCTGCAGTTC TACATCTCCA GAGAGTGGCT CAACAAATTC 
AACACATTCA CTGAGCCCGG ACCCATCACC AATCACACTT TCCTTTGTCA GCACGGAGGA 
ATTCCTCCTA CGAAGTACCA TTATGTGGAT GATCTGGTTG TGATCCTTCC TCAAAATGTG 
TGGGAATATC TCTACAACAG GTTTGGAGGT GGCCCGGCTG TGAATCACTT ATATGTGTGT 
GCAATTTGCC AAGTGGAGAT CGAGACCTTA GCCAAACGAC GGAAACTGGA GATAGACACC 
TTCATTAAGT TAAATAAGGA GTTTCAGGCT GAAGAAGCTC CTACCGTCAT CCTTTGTATA 
AGCATGCAGT GGTTCAGAGA ATGGGAGAAC TTTGTCAAGG GCAAAGACAA TGAGCCACCA 
GGCCCCATTG ACAACAGCAA GATTGCAGTA ATGAAAGGCG GTCATATCCA GCTTAAACAA 
GGAGCAGACT ATGGGCAGAT ATCTGAAGAA ACGTGGCAGT ACTTGTTAAG TATTTATGGC 
GGTGGGCCAG AGATTGCAGT GCGGCAAACC ATCAGCCCCC CAGACACTGA CACACACGGG 
GAGAGGAAAA TAGAGGCAGA GACCAGAGCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299733.1
CDS	172..2907
Translation	MTDSGDLCPHLDSIGEVTKEELIQKSKGTCQSCGVGGPNLWACLQCDCPYVGCGESYSDHSTIHAQAKKHNLTVNLTTFRVWCYVCEREVFLEPKPVTPVSSAHRCKPHDQDPVSQTTCYPLKAVPIAVADEEGSESEEDELKPRGLTGMKNIGNSCYMNAALQALSNCPPLTQFFQDCSGLVRTDKKPALCKSYQKLISELWHKKRPSYVVPTTLFHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPLFDCSGGISEVEPDLSLDSCNLVDGDRSPSEDEFLSCDSGSGSERGDGERAGGEAELLIQDECVAVRGTGGISEKERLKERRGEERTREMDEDADVDTAAQDGQAERETETATPATAVPAPGNTEPDNEASMHCPSSRPCSPAHSVQELHSRLSSNPPRSSPLRTGPTYTFKKAQMLLSTKKKKQSRFRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSSIHQSAPVKAGVCTDGYAAQGWISYIMDSIRRFVVSCIPSWFWGPMVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLKPFLAKESPSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEAYVLFYRKSSEESVRERQRVVALANLKEPSLLQFYISREWLNKFNTFTEPGPITNHTFLCQHGGIPPTKYHYVDDLVVILPQNVWEYLYNRFGGGPAVNHLYVCAICQVEIETLAKRRKLEIDTFIKLNKEFQAEEAPTVILCISMQWFREWENFVKGKDNEPPGPIDNSKIAVMKGGHIQLKQGADYGQISEETWQYLLSIYGGGPEIAVRQTISPPDTDTHGERKIEAETRAL

Target ORF information:

RefSeq Version	XM_009301458.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 20 (usp20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301458.3

ORF Insert Sequence:

ATGACTGACA GCGGGGATCT TTGTCCTCAC TTGGACTCGA TTGGTGAAGT TACTAAAGAA 
GAACTGATTC AGAAATCAAA GGGCACGTGT CAGTCATGTG GAGTAGGTGG ACCAAATCTC 
TGGGCATGTC TGCAGTGTGA CTGTCCTTAT GTAGGCTGTG GAGAGTCGTA TTCAGACCAC 
AGTACTATTC ATGCACAGGC CAAAAAGCAC AATCTGACAG TAAACCTGAC CACATTCAGG 
GTGTGGTGTT ATGTCTGCGA AAGAGAGGTG TTTCTGGAAC CGAAGCCTGT TACTCCTGTA 
TCATCAGCTC ACCGCTGCAA ACCACATGAC CAGGATCCTG TTTCGCAGAC TACATGTTAC 
CCATTGAAGG CGGTGCCTAT CGCAGTGGCA GATGAGGAGG GCTCAGAGTC TGAGGAGGAT 
GAGCTCAAAC CTAGAGGTCT GACAGGGATG AAAAACATTG GAAACTCTTG TTATATGAAT 
GCTGCACTGC AGGCCTTGTC CAACTGTCCT CCTTTGACTC AGTTTTTCCA GGATTGCAGT 
GGATTGGTGC GTACGGATAA GAAGCCGGCA TTGTGTAAAA GCTATCAGAA GCTTATCTCT 
GAACTCTGGC ACAAGAAACG GCCGAGTTAT GTTGTGCCAA CTACTCTGTT TCATGGAATC 
AAACTGGTGA ACCCCATGTT TCGTGGCTAT GCTCAACAGG ACACTCAGGA GTTTTTGCGA 
TGTCTGATGG ACCAGCTTCA CGAGGAGCTG AAGGAGCCTT TGTTTGACTG CAGCGGTGGC 
ATCTCTGAAG TCGAGCCTGA CCTCAGTCTG GACAGCTGTA ATCTTGTGGA CGGAGACCGA 
AGCCCCTCTG AAGACGAATT CCTGTCGTGT GACTCTGGAT CAGGAAGCGA AAGGGGAGAT 
GGAGAGCGTG CAGGAGGAGA AGCAGAGCTC CTCATTCAAG ATGAATGTGT GGCAGTGAGA 
GGAACCGGAG GCATCTCGGA GAAAGAGAGG CTGAAGGAGA GAAGAGGAGA AGAGAGGACA 
CGTGAAATGG ATGAGGATGC AGATGTGGAC ACAGCTGCAC AAGACGGACA GGCTGAGAGA 
GAAACTGAAA CAGCGACACC AGCCACAGCT GTGCCGGCAC CAGGAAACAC AGAACCTGAT 
AATGAAGCTT CCATGCACTG TCCATCCTCT CGCCCCTGTA GCCCCGCTCA CAGTGTACAG 
GAGCTTCACT CCAGACTCTC TAGTAACCCA CCAAGATCCA GCCCTCTCAG AACTGGACCC 
ACGTATACTT TCAAGAAAGC TCAGATGCTA CTGAGCACCA AGAAGAAGAA GCAGTCCCGT 
TTCCGTAGTG TTATTTCCGA TATCTTTGAT GGATCCATTC TGAGTCTTGT TCAGTGTTTG 
ACATGTGACC GGGTTTCGAC AACTGTGGAG ACGTTTCAGG ACCTTTCTTT GCCTATACCC 
GGTAAAGAAG ATTTGGCTAA GCTTCATTCC TCCATTCACC AGAGTGCACC TGTTAAAGCT 
GGTGTCTGCA CTGATGGCTA TGCTGCACAG GGCTGGATCT CCTACATCAT GGACTCTATA 
CGAAGGTTTG TAGTGTCCTG TATTCCAAGT TGGTTTTGGG GCCCCATGGT AACGCTAGAG 
GACTGTCTGG CAGCTTTCTT TGCTGCTGAT GAGCTGAAAG GAGACAACAT GTATAGCTGT 
GAACGGTGTA AAAAATTAAG AAATGGTGTG AAATATTGTA AAGTTCTGCG TCTACCAGAA 
ATATTGTGCA TTCACCTGAA GCGCTTCAGA CATGAAGTCA TGTACTCGTT CAAAATAAAT 
AGCCACGTGT CATTCCCATT GGAGGGTTTG GATCTCAAAC CGTTCCTGGC TAAGGAAAGC 
CCGTCCCAGA TTACTACCTA TGACCTGCTG TCTGTCATCT GTCATCACGG CACTGCTGGA 
AGTGGGCATT ACATAGCGTA CTGCCAGAAT GTGATCAATG GACAGTGGTA CGAGTTTGAC 
GATCAGTATG TAACAGAAGT GCACGAGACA GTAGTCCAGA ATGCTGAAGC ATATGTCCTA 
TTCTACAGGA AGAGCAGTGA AGAATCTGTG AGAGAAAGAC AAAGGGTTGT CGCTCTGGCT 
AATTTGAAGG AGCCCAGTTT GCTGCAGTTC TACATCTCCA GAGAGTGGCT CAACAAATTC 
AACACATTCA CTGAGCCCGG ACCCATCACC AATCACACTT TCCTTTGTCA GCACGGAGGA 
ATTCCTCCTA CGAAGTACCA TTATGTGGAT GATCTGGTTG TGATCCTTCC TCAAAATGTG 
TGGGAATATC TCTACAACAG GTTTGGAGGT GGCCCGGCTG TGAATCACTT ATATGTGTGT 
GCAATTTGCC AAGTGGAGAT CGAGACCTTA GCCAAACGAC GGAAACTGGA GATAGACACC 
TTCATTAAGT TAAATAAGGA GTTTCAGGCT GAAGAAGCTC CTACCGTCAT CCTTTGTATA 
AGCATGCAGT GGTTCAGAGA ATGGGAGAAC TTTGTCAAGG GCAAAGACAA TGAGCCACCA 
GGCCCCATTG ACAACAGCAA GATTGCAGTA ATGAAAGGCG GTCATATCCA GCTTAAACAA 
GGAGCAGACT ATGGGCAGAT ATCTGAAGAA ACGTGGCAGT ACTTGTTAAG TATTTATGGC 
GGTGGGCCAG AGATTGCAGT GCGGCAAACC ATCAGCCCCC CAGACACTGA CACACACGGG 
GAGAGGAAAA TAGAGGCAGA GACCAGAGCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Importance of deubiquitinases in zebrafish craniofacial development.
Biochemical and biophysical research communications487(4)813-819(2017 06)
Tse WKF

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ

Positive correlation between gene coexpression and positional clustering in the zebrafish genome.
BMC genomics1042(2009 Jan)
Ng YK,Wu W,Zhang L

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usp20 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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