crnkl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	crnkl1
Entrez Gene ID	393920
Full Name	crooked neck pre-mRNA splicing factor 1
Synonyms	zgc:55327
General protein information	Preferred Names crooked neck pre-mRNA splicing factor 1 Names crooked neck-like protein 1 crooked neck pre-mRNA splicing factor-like 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200946.1	NP_957240.1	crooked neck-like protein 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0523800	NP_001174825.1
Bos taurus (cattle)	CRNKL1	NP_001193451.1
Arabidopsis thaliana (thale cress)	AT3G13210	NP_187927.1
Rattus norvegicus (Norway rat)	LOC102548514	XP_006235227.1
Homo sapiens (human)	CRNKL1	NP_057736.4
Pan troglodytes (chimpanzee)	CRNKL1	XP_514541.2
Gallus gallus (chicken)	CRNKL1	XP_419315.2
Arabidopsis thaliana (thale cress)	AT5G45990	NP_199411.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001879	XP_321188.4
Neurospora crassa	NCU02717	XP_965102.1
Schizosaccharomyces pombe (fission yeast)	cwf4	NP_596573.1
Magnaporthe oryzae (rice blast fungus)	MGG_05720	XP_003710622.1
Oryza sativa (rice)	Os05g0289400	NP_001055097.1
Danio rerio (zebrafish)	crnkl1	NP_957240.1
Caenorhabditis elegans (roundworm)	M03F8.3	NP_001122979.1
Xenopus tropicalis (tropical clawed frog)	crnkl1	NP_001016895.1
Arabidopsis thaliana (thale cress)	AT5G41770	NP_198992.2
Macaca mulatta (Rhesus monkey)	LOC699931	XP_002808010.1
Canis lupus familiaris (dog)	CRNKL1	XP_005634771.1
Mus musculus (house mouse)	Crnkl1	NP_080096.1
Drosophila melanogaster (fruit fly)	crn	NP_477118.1
Rattus norvegicus (Norway rat)	Crnkl1	NP_446249.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions.
Gallagher TL, Arribere JA, Geurts PA, Exner CR, McDonald KL, Dill KK, Marr HL, Adkar SS, Garnett AT, Amacher SL, Conboy JG
Developmental biology359(2)251-61(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crnkl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crnkl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02526	NM_200946.1 Latest version!	Danio rerio crooked neck pre-mRNA splicing factor 1 (crnkl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crnkl1 ( NM_200946.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957240.1 Danio rerio crooked neck pre-mRNA splicing factor 1 (crnkl1), mRNA.

Price & Availability↑

CloneID	ODa02526
Clone ID Related Accession (Same CDS sequence)	NM_200946.1
Accession Version	NM_200946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	crooked neck-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044369.1. ##Evidence-Data-START## Transcript exon combination :: BC044369.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCCA CCGCGGCGGG CAAACAGAGA ATCCCGAAAG TGGCGAAGGT GAAGAATAAA 
GCCCCTGCAG AGGTGCAGAT CACCGCCGAG CAGCTGCTCA GAGAGGCCAA AGAGAGAGAG 
CTCGAGCTGC TGCCACCGCC ACCCAAACAG AAGATCACCG ATAAAGAAGA GCTCAATGAC 
TACAAACTGA AAAAGAGAAA GGGCTTTGAG GACAACATCA GGAAAAACAG AACTGTGATC 
AGCAACTGGA TCAAGTACGC ACAATGGGAG GAGAGCCTGC AGGAGGTCCA GAGATCTCGC 
TCCATCTACG AGCGTGCACT CGACGTGGAT CACCGCAATA TCACACTGTG GCTGAAGTAT 
GCCGAGATGG AGATGAAGAA CCGGCAGGTC AATCACGCTC GCAACATATG GGACCGAGCC 
ATTACCATCC TGCCCCGCGT CAATCAGTTC TGGTACAAGT ACACGTATAT GGAGGAGATG 
CTGGGCAACA TTGCAGGCTG CAGGCAGGTG TTTGAGCGCT GGATGGAGTG GGAACCGGAG 
GAGCAGGCCT GGCATTCATA CATCAACTTT GAGCTGCGCT ATAAGGAGGT GGACAAGGCC 
CGCAGCATCT ATGAGAATTT TGTGATGGTC CATCCTGAGG TGAAGAACTG GATCAAATAT 
GCCCACTTTG AGGAGAAGCA CGGCTATGTT GCTCGTGGCA GGAAAGTGTT TGAGCGAGCT 
GTGGAGTTTT TCGGTGAAGA ACAGGTCAGC GAGAATCTCT ACGTGGCTTT TGCCAGATTT 
GAGGAGAAGC AGAAAGAGTT TGAGAGAGTT CGAGTCATCT ATAAATACGC TTTAGACCGA 
ATCCCCAAGC AACAGGCCCA GGAGCTGTTC AAGAACTACA CTGTGTTTGA GAAGAGGTTT 
GGAGACAGGA GGGGAATCGA GGACGTGATC GTCAGCAAGA GGAGGTTTCA GTACGAAGAG 
GAGGTCAAAG CTAACCCACA CAATTATGAT GCATGGTTTG ATTACCTGCG TCTGGTGGAG 
AGTGATGCCG ATGCAGACAC AGTTAGAGAA GTGTACGAGA GAGCGATCGC CAACATTCCC 
CCTATTCAAG AGAAGAGACA CTGGAGACGC TACATCTACC TGTGGATTAA CTACGCTCTC 
TATGAGGAGC TGGAGGTCAA GGACCCTGAG AGAACGAGAC AGGTGTACAA GGCCTGTCTG 
GAGCTTATAC CACACAAAAA GTTTACTTTT GCCAAGATTT GGCTCTTATA TGGGCAATTT 
GAAATCCGCC AAAAGAACCT CCAAAATGCA AGACGAGGCC TGGGCACAGC CATTGGAAAG 
TGTCCCAAAA ACAAACTGTT CAAGGGCTAC ATCGAGCTGG AGCTGCAGCT GAGAGAGTTT 
GACCGCTGTA GAAAACTCTA CGAGAAATAT CTGGAGTTCA GTCCGGAAAA CTGCACTACC 
TGGATCAAGT TTGCAGAGCT GGAGACCATC TTGGGGGACA CCGACCGCTC CAGAGCCATA 
TTTGAGCTGG CCATAGGACA GCCACGACTG GACATGCCGG AGGTGTTGTG GAAATCCTAC 
ATTGACTTTG AGATCGAGCA GGAAGAATAC GACAACACAC GAGGACTGTA TAAGAGATTG 
TTACAGCGCA CTCAACATGT CAAAGTCTGG ATCAGCTACG CTCAGTTTGA GCTGTCCATC 
GACACAGAAG ACCGCGTGCA GAGGTGCAGA CAGGTCTACG AGGAGGCCAA TAAGGGCATG 
CAGAACTGCG AGGAGAAAGA GGAGCGTCTG ATGCTCTTGG AGTCCTGGAG AGACTACGAG 
GAGGAGTTCG GATCCTTCAC CCACAAGGAG AGAGTCCGCA AACTCCTGCC GGAGAAGGTG 
AAGAAGAGGA GGAAGATCAC GGCGGAGGAT GGGTCGGATG CTGGTTGGGA AGAATATTAC 
GACTACATCT TCCCCGAGGA CGCTGCCAAC CAGCCTAACC TCAAACTGCT GGCCATGGCC 
AAGATGTGGA AGAAACAGCA GCAGCAGGAG GAGGAGGATG ATGACGAGGA GGAAGATGAA 
AACGATAAGG AAGAACAGCA GGTTCCTGAA GGTAAGGACG CACAGGAGAG TCCAGAAGCA 
GAGGAGCAGG AGCCTGCCGC TGGTGGAGAA CAGGCAGAGA AAGAGTATGA TGACCGCGAT 
GATGAAGACG AGAGCAGCAG CAGCAGCAGC TCCGACAGCG AGAGTGATGA AGACCAGAAA 
GATAAGACTG AACATAACAA GCCGGACACA GAACAACAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957240.1
CDS	52..2313
Translation	MASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPKQKITDKEELNDYKLKKRKGFEDNIRKNRTVISNWIKYAQWEESLQEVQRSRSIYERALDVDHRNITLWLKYAEMEMKNRQVNHARNIWDRAITILPRVNQFWYKYTYMEEMLGNIAGCRQVFERWMEWEPEEQAWHSYINFELRYKEVDKARSIYENFVMVHPEVKNWIKYAHFEEKHGYVARGRKVFERAVEFFGEEQVSENLYVAFARFEEKQKEFERVRVIYKYALDRIPKQQAQELFKNYTVFEKRFGDRRGIEDVIVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDADADTVREVYERAIANIPPIQEKRHWRRYIYLWINYALYEELEVKDPERTRQVYKACLELIPHKKFTFAKIWLLYGQFEIRQKNLQNARRGLGTAIGKCPKNKLFKGYIELELQLREFDRCRKLYEKYLEFSPENCTTWIKFAELETILGDTDRSRAIFELAIGQPRLDMPEVLWKSYIDFEIEQEEYDNTRGLYKRLLQRTQHVKVWISYAQFELSIDTEDRVQRCRQVYEEANKGMQNCEEKEERLMLLESWRDYEEEFGSFTHKERVRKLLPEKVKKRRKITAEDGSDAGWEEYYDYIFPEDAANQPNLKLLAMAKMWKKQQQQEEEDDDEEEDENDKEEQQVPEGKDAQESPEAEEQEPAAGGEQAEKEYDDRDDEDESSSSSSSDSESDEDQKDKTEHNKPDTEQQ

Target ORF information:

RefSeq Version	NM_200946.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio crooked neck pre-mRNA splicing factor 1 (crnkl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200946.1

ORF Insert Sequence:

ATGGCGTCCA CCGCGGCGGG CAAACAGAGA ATCCCGAAAG TGGCGAAGGT GAAGAATAAA 
GCCCCTGCAG AGGTGCAGAT CACCGCCGAG CAGCTGCTCA GAGAGGCCAA AGAGAGAGAG 
CTCGAGCTGC TGCCACCGCC ACCCAAACAG AAGATCACCG ATAAAGAAGA GCTCAATGAC 
TACAAACTGA AAAAGAGAAA GGGCTTTGAG GACAACATCA GGAAAAACAG AACTGTGATC 
AGCAACTGGA TCAAGTACGC ACAATGGGAG GAGAGCCTGC AGGAGGTCCA GAGATCTCGC 
TCCATCTACG AGCGTGCACT CGACGTGGAT CACCGCAATA TCACACTGTG GCTGAAGTAT 
GCCGAGATGG AGATGAAGAA CCGGCAGGTC AATCACGCTC GCAACATATG GGACCGAGCC 
ATTACCATCC TGCCCCGCGT CAATCAGTTC TGGTACAAGT ACACGTATAT GGAGGAGATG 
CTGGGCAACA TTGCAGGCTG CAGGCAGGTG TTTGAGCGCT GGATGGAGTG GGAACCGGAG 
GAGCAGGCCT GGCATTCATA CATCAACTTT GAGCTGCGCT ATAAGGAGGT GGACAAGGCC 
CGCAGCATCT ATGAGAATTT TGTGATGGTC CATCCTGAGG TGAAGAACTG GATCAAATAT 
GCCCACTTTG AGGAGAAGCA CGGCTATGTT GCTCGTGGCA GGAAAGTGTT TGAGCGAGCT 
GTGGAGTTTT TCGGTGAAGA ACAGGTCAGC GAGAATCTCT ACGTGGCTTT TGCCAGATTT 
GAGGAGAAGC AGAAAGAGTT TGAGAGAGTT CGAGTCATCT ATAAATACGC TTTAGACCGA 
ATCCCCAAGC AACAGGCCCA GGAGCTGTTC AAGAACTACA CTGTGTTTGA GAAGAGGTTT 
GGAGACAGGA GGGGAATCGA GGACGTGATC GTCAGCAAGA GGAGGTTTCA GTACGAAGAG 
GAGGTCAAAG CTAACCCACA CAATTATGAT GCATGGTTTG ATTACCTGCG TCTGGTGGAG 
AGTGATGCCG ATGCAGACAC AGTTAGAGAA GTGTACGAGA GAGCGATCGC CAACATTCCC 
CCTATTCAAG AGAAGAGACA CTGGAGACGC TACATCTACC TGTGGATTAA CTACGCTCTC 
TATGAGGAGC TGGAGGTCAA GGACCCTGAG AGAACGAGAC AGGTGTACAA GGCCTGTCTG 
GAGCTTATAC CACACAAAAA GTTTACTTTT GCCAAGATTT GGCTCTTATA TGGGCAATTT 
GAAATCCGCC AAAAGAACCT CCAAAATGCA AGACGAGGCC TGGGCACAGC CATTGGAAAG 
TGTCCCAAAA ACAAACTGTT CAAGGGCTAC ATCGAGCTGG AGCTGCAGCT GAGAGAGTTT 
GACCGCTGTA GAAAACTCTA CGAGAAATAT CTGGAGTTCA GTCCGGAAAA CTGCACTACC 
TGGATCAAGT TTGCAGAGCT GGAGACCATC TTGGGGGACA CCGACCGCTC CAGAGCCATA 
TTTGAGCTGG CCATAGGACA GCCACGACTG GACATGCCGG AGGTGTTGTG GAAATCCTAC 
ATTGACTTTG AGATCGAGCA GGAAGAATAC GACAACACAC GAGGACTGTA TAAGAGATTG 
TTACAGCGCA CTCAACATGT CAAAGTCTGG ATCAGCTACG CTCAGTTTGA GCTGTCCATC 
GACACAGAAG ACCGCGTGCA GAGGTGCAGA CAGGTCTACG AGGAGGCCAA TAAGGGCATG 
CAGAACTGCG AGGAGAAAGA GGAGCGTCTG ATGCTCTTGG AGTCCTGGAG AGACTACGAG 
GAGGAGTTCG GATCCTTCAC CCACAAGGAG AGAGTCCGCA AACTCCTGCC GGAGAAGGTG 
AAGAAGAGGA GGAAGATCAC GGCGGAGGAT GGGTCGGATG CTGGTTGGGA AGAATATTAC 
GACTACATCT TCCCCGAGGA CGCTGCCAAC CAGCCTAACC TCAAACTGCT GGCCATGGCC 
AAGATGTGGA AGAAACAGCA GCAGCAGGAG GAGGAGGATG ATGACGAGGA GGAAGATGAA 
AACGATAAGG AAGAACAGCA GGTTCCTGAA GGTAAGGACG CACAGGAGAG TCCAGAAGCA 
GAGGAGCAGG AGCCTGCCGC TGGTGGAGAA CAGGCAGAGA AAGAGTATGA TGACCGCGAT 
GATGAAGACG AGAGCAGCAG CAGCAGCAGC TCCGACAGCG AGAGTGATGA AGACCAGAAA 
GATAAGACTG AACATAACAA GCCGGACACA GAACAACAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions.
Developmental biology359(2)251-61(2011 Nov)
Gallagher TL,Arribere JA,Geurts PA,Exner CR,McDonald KL,Dill KK,Marr HL,Adkar SS,Garnett AT,Amacher SL,Conboy JG

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crnkl1 cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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