pcbd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pcbd1
Entrez Gene ID	393787
Full Name	pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
Synonyms	pcbd,zgc:73389
General protein information	Preferred Names pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha Names pterin-4-alpha-carbinolamine dehydratase 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200814.1	NP_957108.1	pterin-4-alpha-carbinolamine dehydratase

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Pcd	NP_477360.2
Pan troglodytes (chimpanzee)	PCBD1	XP_001171466.1
Xenopus tropicalis (tropical clawed frog)	pcbd1	NP_001011016.1
Gallus gallus (chicken)	PCBD1	NP_990236.1
Homo sapiens (human)	PCBD1	NP_000272.1
Macaca mulatta (Rhesus monkey)	PCBD1	XP_001107537.1
Bos taurus (cattle)	PCBD1	NP_001193403.1
Danio rerio (zebrafish)	pcbd1	NP_957108.1
Canis lupus familiaris (dog)	PCBD1	XP_005619043.1
Mus musculus (house mouse)	Pcbd1	NP_079549.1
Rattus norvegicus (Norway rat)	Pcbd1	NP_001007602.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Characterization of a BAC library from channel catfish Ictalurus punctatus: indications of high levels of chromosomal reshuffling among teleost genomes.
Wang S, Xu P, Thorsen J, Zhu B, de Jong PJ, Waldbieser G, Kucuktas H, Liu Z
Marine biotechnology (New York, N.Y.)9(6)701-11(2007 Nov-Dec)

XBP-1, a key regulator of unfolded protein response, activates transcription of IGF1 and Akt phosphorylation in zebrafish embryonic cell line.
Hu MC, Gong HY, Lin GH, Hu SY, Chen MH, Huang SJ, Liao CF, Wu JL
Biochemical and biophysical research communications359(3)778-83(2007 Aug)

Evolution of pigment synthesis pathways by gene and genome duplication in fish.
Braasch I, Schartl M, Volff JN
BMC evolutionary biology774(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pcbd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pcbd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03629	NM_200814.1 Latest version!	Danio rerio pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (pcbd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pcbd1 ( NM_200814.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957108.1 Danio rerio pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (pcbd1), mRNA.

Price & Availability↑

CloneID	ODa03629
Clone ID Related Accession (Same CDS sequence)	NM_200814.1
Accession Version	NM_200814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	pterin-4-alpha-carbinolamine dehydratase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC059694.1. ##Evidence-Data-START## Transcript exon combination :: EH496062.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGGGA AGATTCAGAC TCTGACTATG GAGGAACGGG AACATCTCCT GCCCATGATG 
AGGAATGCGC AGTGGGTGGA GGTGGGGGGC AGAGATGCCA TTTACAAAGA GTTCATCTTC 
AAAGACTTCA ACCAGGCTTT TGGGTTCATG TCCAGAGTTG CTTTACAGGC TGAGAAAATG 
GATCATCATC CTGAATGGTT TAATGTGTAT AATAAGGTTC AGATCACCCT CAGCACTCAC 
GAATGTGGAG GACTCTCACA GCGAGACATC AACCTCGCCA CCTTCATAGA CCAGGCCTCT 
GTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957108.1
CDS	20..328
Translation	MAGKIQTLTMEEREHLLPMMRNAQWVEVGGRDAIYKEFIFKDFNQAFGFMSRVALQAEKMDHHPEWFNVYNKVQITLSTHECGGLSQRDINLATFIDQASVL

Target ORF information:

RefSeq Version	NM_200814.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (pcbd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200814.1

ORF Insert Sequence:

ATGGCAGGGA AGATTCAGAC TCTGACTATG GAGGAACGGG AACATCTCCT GCCCATGATG 
AGGAATGCGC AGTGGGTGGA GGTGGGGGGC AGAGATGCCA TTTACAAAGA GTTCATCTTC 
AAAGACTTCA ACCAGGCTTT TGGGTTCATG TCCAGAGTTG CTTTACAGGC TGAGAAAATG 
GATCATCATC CTGAATGGTT TAATGTGTAT AATAAGGTTC AGATCACCCT CAGCACTCAC 
GAATGTGGAG GACTCTCACA GCGAGACATC AACCTCGCCA CCTTCATAGA CCAGGCCTCT 
GTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Characterization of a BAC library from channel catfish Ictalurus punctatus: indications of high levels of chromosomal reshuffling among teleost genomes.
Marine biotechnology (New York, N.Y.)9(6)701-11(2007 Nov-Dec)
Wang S,Xu P,Thorsen J,Zhu B,de Jong PJ,Waldbieser G,Kucuktas H,Liu Z

XBP-1, a key regulator of unfolded protein response, activates transcription of IGF1 and Akt phosphorylation in zebrafish embryonic cell line.
Biochemical and biophysical research communications359(3)778-83(2007 Aug)
Hu MC,Gong HY,Lin GH,Hu SY,Chen MH,Huang SJ,Liao CF,Wu JL

Evolution of pigment synthesis pathways by gene and genome duplication in fish.
BMC evolutionary biology774(2007 May)
Braasch I,Schartl M,Volff JN