dpm3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dpm3
Entrez Gene ID	393782
Full Name	dolichyl-phosphate mannosyltransferase polypeptide 3
Synonyms	zgc:73376
General protein information	Preferred Names dolichyl-phosphate mannosyltransferase polypeptide 3 Names dolichol-phosphate mannosyltransferase subunit 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159422.2	XP_005159479.1	dolichol-phosphate mannosyltransferase subunit 3 isoform X1
XM_005159422.3	XP_005159479.1	dolichol-phosphate mannosyltransferase subunit 3 isoform X1
NM_200809.2	NP_957103.2	dolichol-phosphate mannosyltransferase subunit 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	DPM3	NP_061846.2
Macaca mulatta (Rhesus monkey)	DPM3	XP_001115270.1
Pan troglodytes (chimpanzee)	DPM3	XP_003308482.1
Bos taurus (cattle)	DPM3	NP_001029532.1
Canis lupus familiaris (dog)	DPM3	XP_003434943.1
Danio rerio (zebrafish)	dpm3	NP_957103.2
Mus musculus (house mouse)	Dpm3	NP_081043.1

Gene Ontology
Bibliography

Related articles in PubMed

Dolichol-phosphate mannose synthase depletion in zebrafish leads to dystrophic muscle with hypoglycosylated α-dystroglycan.
Marchese M, Pappalardo A, Baldacci J, Verri T, Doccini S, Cassandrini D, Bruno C, Fiorillo C, Garcia-Gil M, Bertini E, Pitto L, Santorelli FM
Biochemical and biophysical research communications477(1)137-143(2016 08)

trappc11 is required for protein glycosylation in zebrafish and humans.
DeRossi C, Vacaru A, Rafiq R, Cinaroglu A, Imrie D, Nayar S, Baryshnikova A, Milev MP, Stanga D, Kadakia D, Gao N, Chu J, Freeze HH, Lehrman MA, Sacher M, Sadler KC
Molecular biology of the cell27(8)1220-34(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dpm3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dpm3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23874	NM_200809.2 Latest version!	Danio rerio dolichyl-phosphate mannosyltransferase subunit 3, regulatory (dpm3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa23874	XM_005159422.3 Latest version!	Danio rerio dolichyl-phosphate mannosyltransferase polypeptide 3 (dpm3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
ODa23874	XM_005159422.2	Danio rerio dolichyl-phosphate mannosyltransferase polypeptide 3 (dpm3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dpm3 ( NM_200809.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957103.2 Danio rerio dolichyl-phosphate mannosyltransferase subunit 3, regulatory (dpm3), mRNA.

Price & Availability↑

CloneID	ODa23874
Clone ID Related Accession (Same CDS sequence)	XM_005159422.2 , XM_005159422.3 , NM_200809.2
Accession Version	NM_200809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1558195200000
Organism	Danio rerio(zebrafish)
Product	dolichol-phosphate mannosyltransferase subunit 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX469895.7. On or before Mar 12, 2008 this sequence version replaced XM_001340990.1, NM_200809.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01008939.1, GDQQ01012660.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACCAAGC TTCTGCAGTG GCTTCTTGCG GTGTCTCTGA TTGGCATTGC ATGGGGACTC 
GTTACCTTCG ACCTCCTGGA TTTGCATTTG CTGCCCCAGT ATAAAGAGCT TGCATGGCCG 
ATGCCCGTGT ACCTGCTGGT GGTGTTTGGG TGTTATTCTC TGGCCACTGT CGGCTACCGT 
GTGGCTACAT TTAATGACTG TGAGGATGCG GCCAAAGAGC TGCAAGCGCA AATAAAAGAA 
GCCAAAGAGG ACTTGAGGAA GAAAGGACTG AAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957103.2
CDS	102..380
Translation	MTKLLQWLLAVSLIGIAWGLVTFDLLDLHLLPQYKELAWPMPVYLLVVFGCYSLATVGYRVATFNDCEDAAKELQAQIKEAKEDLRKKGLKM

Target ORF information:

RefSeq Version	NM_200809.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dolichyl-phosphate mannosyltransferase subunit 3, regulatory (dpm3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200809.2

ORF Insert Sequence:

ATGACCAAGC TTCTGCAGTG GCTTCTTGCG GTGTCTCTGA TTGGCATTGC ATGGGGACTC 
GTTACCTTCG ACCTCCTGGA TTTGCATTTG CTGCCCCAGT ATAAAGAGCT TGCATGGCCG 
ATGCCCGTGT ACCTGCTGGT GGTGTTTGGG TGTTATTCTC TGGCCACTGT CGGCTACCGT 
GTGGCTACAT TTAATGACTG TGAGGATGCG GCCAAAGAGC TGCAAGCGCA AATAAAAGAA 
GCCAAAGAGG ACTTGAGGAA GAAAGGACTG AAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dpm3 ( XM_005159422.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159479.1 Danio rerio dolichyl-phosphate mannosyltransferase polypeptide 3 (dpm3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23874
Clone ID Related Accession (Same CDS sequence)	XM_005159422.2 , XM_005159422.3 , NM_200809.2
Accession Version	XM_005159422.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	dolichol-phosphate mannosyltransferase subunit 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC TTCTGCAGTG GCTTCTTGCG GTGTCTCTGA TTGGCATTGC ATGGGGACTC 
GTTACCTTCG ACCTCCTGGA TTTGCATTTG CTGCCCCAGT ATAAAGAGCT TGCATGGCCG 
ATGCCCGTGT ACCTGCTGGT GGTGTTTGGG TGTTATTCTC TGGCCACTGT CGGCTACCGT 
GTGGCTACAT TTAATGACTG TGAGGATGCG GCCAAAGAGC TGCAAGCGCA AATAAAAGAA 
GCCAAAGAGG ACTTGAGGAA GAAAGGACTG AAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159479.1
CDS	85..363
Translation	MTKLLQWLLAVSLIGIAWGLVTFDLLDLHLLPQYKELAWPMPVYLLVVFGCYSLATVGYRVATFNDCEDAAKELQAQIKEAKEDLRKKGLKM

Target ORF information:

RefSeq Version	XM_005159422.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dolichyl-phosphate mannosyltransferase polypeptide 3 (dpm3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159422.2

ORF Insert Sequence:

ATGACCAAGC TTCTGCAGTG GCTTCTTGCG GTGTCTCTGA TTGGCATTGC ATGGGGACTC 
GTTACCTTCG ACCTCCTGGA TTTGCATTTG CTGCCCCAGT ATAAAGAGCT TGCATGGCCG 
ATGCCCGTGT ACCTGCTGGT GGTGTTTGGG TGTTATTCTC TGGCCACTGT CGGCTACCGT 
GTGGCTACAT TTAATGACTG TGAGGATGCG GCCAAAGAGC TGCAAGCGCA AATAAAAGAA 
GCCAAAGAGG ACTTGAGGAA GAAAGGACTG AAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dpm3 ( XM_005159422.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159479.1 Danio rerio dolichyl-phosphate mannosyltransferase polypeptide 3 (dpm3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23874
Clone ID Related Accession (Same CDS sequence)	XM_005159422.2 , XM_005159422.3 , NM_200809.2
Accession Version	XM_005159422.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	dolichol-phosphate mannosyltransferase subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC TTCTGCAGTG GCTTCTTGCG GTGTCTCTGA TTGGCATTGC ATGGGGACTC 
GTTACCTTCG ACCTCCTGGA TTTGCATTTG CTGCCCCAGT ATAAAGAGCT TGCATGGCCG 
ATGCCCGTGT ACCTGCTGGT GGTGTTTGGG TGTTATTCTC TGGCCACTGT CGGCTACCGT 
GTGGCTACAT TTAATGACTG TGAGGATGCG GCCAAAGAGC TGCAAGCGCA AATAAAAGAA 
GCCAAAGAGG ACTTGAGGAA GAAAGGACTG AAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159479.1
CDS	89..367
Translation	MTKLLQWLLAVSLIGIAWGLVTFDLLDLHLLPQYKELAWPMPVYLLVVFGCYSLATVGYRVATFNDCEDAAKELQAQIKEAKEDLRKKGLKM

Target ORF information:

RefSeq Version	XM_005159422.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dolichyl-phosphate mannosyltransferase polypeptide 3 (dpm3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159422.3

ORF Insert Sequence:

ATGACCAAGC TTCTGCAGTG GCTTCTTGCG GTGTCTCTGA TTGGCATTGC ATGGGGACTC 
GTTACCTTCG ACCTCCTGGA TTTGCATTTG CTGCCCCAGT ATAAAGAGCT TGCATGGCCG 
ATGCCCGTGT ACCTGCTGGT GGTGTTTGGG TGTTATTCTC TGGCCACTGT CGGCTACCGT 
GTGGCTACAT TTAATGACTG TGAGGATGCG GCCAAAGAGC TGCAAGCGCA AATAAAAGAA 
GCCAAAGAGG ACTTGAGGAA GAAAGGACTG AAGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dolichol-phosphate mannose synthase depletion in zebrafish leads to dystrophic muscle with hypoglycosylated α-dystroglycan.
Biochemical and biophysical research communications477(1)137-143(2016 08)
Marchese M,Pappalardo A,Baldacci J,Verri T,Doccini S,Cassandrini D,Bruno C,Fiorillo C,Garcia-Gil M,Bertini E,Pitto L,Santorelli FM

trappc11 is required for protein glycosylation in zebrafish and humans.
Molecular biology of the cell27(8)1220-34(2016 Apr)
DeRossi C,Vacaru A,Rafiq R,Cinaroglu A,Imrie D,Nayar S,Baryshnikova A,Milev MP,Stanga D,Kadakia D,Gao N,Chu J,Freeze HH,Lehrman MA,Sacher M,Sadler KC

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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dpm3 cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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