otud6b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	otud6b
Entrez Gene ID	393194
Full Name	OTU domain containing 6B
Synonyms	zgc:56305
General protein information	Preferred Names OTU domain containing 6B Names OTU domain-containing protein 6B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017352202.1	XP_017207691.1	OTU domain-containing protein 6B isoform X1
NM_200225.1	NP_956519.1	deubiquitinase OTUD6B

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	OTUD6B	XP_001086186.2
Mus musculus (house mouse)	Otud6b	NP_690025.2
Arabidopsis thaliana (thale cress)	AT3G62940	NP_850739.1
Danio rerio (zebrafish)	otud6b	NP_956519.1
Xenopus tropicalis (tropical clawed frog)	otud6b	NP_001011269.1
Pan troglodytes (chimpanzee)	OTUD6B	XP_003311873.1
Rattus norvegicus (Norway rat)	Otud6b	NP_001100109.1
Bos taurus (cattle)	OTUD6B	XP_005228655.1
Homo sapiens (human)	OTUD6B	NP_057107.3
Drosophila melanogaster (fruit fly)	CG7857	NP_648769.2
Caenorhabditis elegans (roundworm)	F21D5.2	NP_001255318.1
Oryza sativa (rice)	Os04g0619500	NP_001053899.1
Canis lupus familiaris (dog)	OTUD6B	XP_544171.2
Gallus gallus (chicken)	OTUD6B	NP_001006347.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006160	XP_316218.4

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following otud6b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the otud6b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38615	NM_200225.1 Latest version!	Danio rerio OTU deubiquitinase 6B (otud6b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa56040	XM_017352202.1 Latest version!	Danio rerio OTU domain containing 6B (otud6b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

otud6b ( NM_200225.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956519.1 Danio rerio OTU deubiquitinase 6B (otud6b), mRNA.

Price & Availability↑

CloneID	ODa38615
Clone ID Related Accession (Same CDS sequence)	NM_200225.1
Accession Version	NM_200225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565452800000
Organism	Danio rerio(zebrafish)
Product	deubiquitinase OTUD6B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC046060.1. ##Evidence-Data-START## Transcript exon combination :: BC046060.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAAG TGGAGACAGC AGAGGAGCAG TTAGCCAAAC AGCATCGCAA GGAAAAGAAA 
GACCTGCAAG CAAAAATACA AAGCATGAAA AACGCAGTTC CCAAAAATGA CAAGAAGAGG 
AGGAAACAGC TGACAGAAGA CATCGCCAAA CTAGAAGCCG AACTCTCTCA AAAACATGAA 
AATGAGCTCA AACTTCAGAA CACTTCTTCA GTGGAAGAGG TTTCAGATGC ATTGGATTCT 
ATGAGTGTAG CAAATCATGA GGAACAATCT GATCCAAGCA AGCAAAGTCG GACATCCAAA 
GCCCAGAAGA GGCGGGACAA AAAGGCTGCT CTTGAGAAGG AGCGAGAGAT GCGGATTGCG 
GAGGCAGAGG TGGAGAATCT CAGCGGCTCT CGTCATCAGG AGGGTCTAAA GCTGCGTGAG 
AAGCTGGTGG AGAGACATCT GCAGATTAAA GAGATCTCTT CTGATGGCCA CTGTATGTAC 
CGCGCTGTGG AGCATCAGCT GACGGAGAGA GGACTTGCAC TCGGTCTGAA AGAGCTTCGG 
GATCAAACTG CTCAGTACAT GAGGAGCCAT GCAGATGACT TCATGCCCTT TCTTACAAAC 
CCTAATACAG GAGATATGTA CACTGCAGAG GAGTTTGAGA AATATTGCAG TGATGTTGCA 
GACACCGCTG CATGGGGCGG ACAGTTAGAG TTGAAAGCCC TTTCCCAGGT CCTCCAGCTA 
CCCATAGAGG TCATCCAGGC AGATTCCCCG TGTATTACTA TTGGTGAAGA ATACGACAAG 
CCAAAAATCA CACTCATTTA TATGCGGCAT GCGTATGGAC TTGGAGAGCA TTACAACTCT 
GTGGAGCCTC TGAAAGATTT GGCAAACGAG GAAGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956519.1
CDS	167..1048
Translation	MEEVETAEEQLAKQHRKEKKDLQAKIQSMKNAVPKNDKKRRKQLTEDIAKLEAELSQKHENELKLQNTSSVEEVSDALDSMSVANHEEQSDPSKQSRTSKAQKRRDKKAALEKEREMRIAEAEVENLSGSRHQEGLKLREKLVERHLQIKEISSDGHCMYRAVEHQLTERGLALGLKELRDQTAQYMRSHADDFMPFLTNPNTGDMYTAEEFEKYCSDVADTAAWGGQLELKALSQVLQLPIEVIQADSPCITIGEEYDKPKITLIYMRHAYGLGEHYNSVEPLKDLANEEEG

Target ORF information:

RefSeq Version	NM_200225.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio OTU deubiquitinase 6B (otud6b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200225.1

ORF Insert Sequence:

ATGGAGGAAG TGGAGACAGC AGAGGAGCAG TTAGCCAAAC AGCATCGCAA GGAAAAGAAA 
GACCTGCAAG CAAAAATACA AAGCATGAAA AACGCAGTTC CCAAAAATGA CAAGAAGAGG 
AGGAAACAGC TGACAGAAGA CATCGCCAAA CTAGAAGCCG AACTCTCTCA AAAACATGAA 
AATGAGCTCA AACTTCAGAA CACTTCTTCA GTGGAAGAGG TTTCAGATGC ATTGGATTCT 
ATGAGTGTAG CAAATCATGA GGAACAATCT GATCCAAGCA AGCAAAGTCG GACATCCAAA 
GCCCAGAAGA GGCGGGACAA AAAGGCTGCT CTTGAGAAGG AGCGAGAGAT GCGGATTGCG 
GAGGCAGAGG TGGAGAATCT CAGCGGCTCT CGTCATCAGG AGGGTCTAAA GCTGCGTGAG 
AAGCTGGTGG AGAGACATCT GCAGATTAAA GAGATCTCTT CTGATGGCCA CTGTATGTAC 
CGCGCTGTGG AGCATCAGCT GACGGAGAGA GGACTTGCAC TCGGTCTGAA AGAGCTTCGG 
GATCAAACTG CTCAGTACAT GAGGAGCCAT GCAGATGACT TCATGCCCTT TCTTACAAAC 
CCTAATACAG GAGATATGTA CACTGCAGAG GAGTTTGAGA AATATTGCAG TGATGTTGCA 
GACACCGCTG CATGGGGCGG ACAGTTAGAG TTGAAAGCCC TTTCCCAGGT CCTCCAGCTA 
CCCATAGAGG TCATCCAGGC AGATTCCCCG TGTATTACTA TTGGTGAAGA ATACGACAAG 
CCAAAAATCA CACTCATTTA TATGCGGCAT GCGTATGGAC TTGGAGAGCA TTACAACTCT 
GTGGAGCCTC TGAAAGATTT GGCAAACGAG GAAGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

otud6b ( XM_017352202.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207691.1 Danio rerio OTU domain containing 6B (otud6b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56040
Clone ID Related Accession (Same CDS sequence)	XM_017352202.1
Accession Version	XM_017352202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	OTU domain-containing protein 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA ATGAAACGAT CGACAGGCCT GCCGAGCCGT CCGTAGAAAT AAACACTATC 
GAACATGATA ATGATGCGCA AACATCAAAA GAAAAACGCG TTGTTAAACT GACTGCTAAA 
GCGCTCGCTG ATAAACTGGG ACGTTTGCAA AATGAAAGGA AAACTAAGCT GAATAAGGCA 
GCCAGTTTAA GAAATGTGTT GAATGGTTTG ATTGAAAAGG GAAATGTTGT ACAAGCTCAA 
TGTGTATTTG ATGATTTTAT TAAGGTCTGT GATGAATCAA AAGGTGTTCA TGAGTGTTTG 
ATGGGTATGT TACCTGATAC TGAACAAGAA AAACATGATG TGTGGTTTAA AGCCAAAATG 
ATCGCCAATA ATGAGCTCAT TTCTAAGGTG CAATTGTGGT TATCAAATTC TGGTCAAAAT 
GTGAGTGTTG GTCCTACTGG TAGCCAAATT GGTGATGGTA TTAATCCAGA GGATAGTGTT 
TCAAATGCAA CTAGTAAACG CTCAGGGAAA AGTGCAGGTA GTAAATCAAG CACTACTTCT 
TCTGTGCGAA TTAAAGCACA AGCGGAAAAG GCTGCATTGC TTGCTCGTAT AGCAGCTTTA 
AAGGAAAAGC ATGCATTGGA AGAGCAAGAG CAGCAGATAA AGAGAAAATT AGAGCAACAG 
GAGTTGGATA CTATGTTGGC TGAAACTGCT GCCAGACTGT CCGTGCTCGA AGCTTCTGAT 
AATTTCTGCA GTGAAAATGC ATCTGATACT ATGAATTCTT ACTTGACGAG AGAAACACAA 
AGTTTAGCAC CTCCACACAA ACTGGACCCG ATGGCAAGTG AATTTAAATG TGCGCCTCAA 
AGTAAACCAC AACAAACACA GGAGTGGACA TCATCATCAA CAACCCCCCC ACTTTTAGCC 
GGAGCGCTAC ATCAAGCTAG TCAACAGCCC ACCACTAGAC CAAAGGAGTG GTCTGATTTA 
AATAAACAAA CAACTGAAAT CAACCGAAAT TTACATCAGC AATCAACCCA ACTTAATGTG 
CCAAATACAA ATCAAAGGTA TACAGGAGGA TCTAGTTCAG GGGTGCACTG GAGGAATAAT 
ACAACATCAC TTGTTCAACA ACAGCATTTG ATATCCTTGC CAGCAAGAAA TATCCCTTTG 
TTCGATGGAG ATCCTCTACA ATATGTCTCC TTCATGAGAG CTTTTGAACA AGGAGTGGAA 
GAAAAGGCTA GTAAGAGCGA CTGTTTGTAC TATCTGGAAC AGTTCACTAG GGGGCAACCA 
AGAGAGCTTG TTCGCAGCTG TTTGTATATG ACGCCTGAGC TAGGTTATGA TAAAGCAAAG 
CAGCTGGTAC AGGAACACTT TGGCTGTAAG TACAAGATTG CTGTGGCGTA CATTGAAAAG 
GCTTTATCTT GGGCTCCAAT AAAGAATGAA GATGTTAATG CTCTACAGGC TTACTCTCTC 
TTTCTGCGAG GCTGCTGCAA TGTTATGGAG GAACTTCAGT ATATGCATGA GCTGGATATG 
CCAAGTAACA TAAGGGCAAT TGTCTTAAAG CTGCCATTTA AACTTCGAGA TCAATGGAGG 
ACTGCTGCAC ATGACTTACA GGAAACCACA GGAAACAGAG CCTTGGTCAA TGACCTGGTG 
AGAGTTATAG AAAGACATGT TCAAATTCTG TCTGATCCAT TGTATGGCAA TATTAGCGAC 
TCACCATCTG CAACAACTGG TCAAAGAGCT GTCAATAGGC CACGAGTATC TCCAGAACAA 
AGAACTAAAG GAAAAAGCTT TGCTACTACC ATTACACCTG TGAAAGTTGA GTGTACCACA 
GGATCAAGGA CCATCTCACC TAGACATAAC TCTGCATCTG CTTTATTCTG TGCATACTGT 
TCTCAGAGTC ATCTATTAGA ACATTGTCAA CAGTTCAAAT GCAAAAAACA CAGAGACAAG 
ATTAATTTTC TGAAGGAGAA ACACCTCTGC TTTGGTTGTT TGAGTACTTC ACACATGAGT 
CGTGACTGTG AGAAACGCCT AACTTGTAAA ATATGCAGCC AAAGCCATCC TACTGTACTT 
CATATTAACA AGAGCGACAC AGCTAGCATG CATAAAGCGC ATGTTGCTAA GGTCACAGTA 
AGTACTTCTA CTCAAATTGA CAAAGATATT ACATCACACT CATTCGCTTC AACTGCTGAG 
ACTTGTGGAC ATACAGGGGC CGGAGCAGAT CAGTGTCTGT TATCCATTGT GCCAGTCCAA 
GTCAAGTCCA TCAAATCTGA TCAGGTAATA CTGACATATG CATTCTTGGA TCCTGGCAGC 
TCCGCCACCT TCTGCTCAGA ACAGCTTATG CAAAAGCTTC ATCTTACTGG GAAACGAACC 
CAGTTCCTCT TATGCACTAT GGGGCAAGAA AGGGTGGTGC CAGCATATTC ACTCACAGGA 
CTGGAAGTGT CTGGTATTAA TGGTAATGTC TTCCATGAAC TTCCTCAAAC CCTAACACAA 
AAGAAAATGC CCGTAAACAC TGATAACATT GTTACTGCTG AAACTCTGGT AAAATGGCCA 
TACCTGGTAG GGTTAACACC TCCCAAGATA GAAGCTAACG TAGACCTGTT GATTGGAAGC 
AACGCACCAA GGTTGTTGGA ACCTTGGGAG GTTATTAATA GCCATGGGAA TGGCCCATAT 
GCTATAAGGA CCGTGTTGGG ATGGGTCATT AATGGTCTTG TGCATGGAGA AGACTCCTGT 
CTTAATGCTG AATGTAGGTC AGCTGTAGTT AACAGGGTTT CAGTATGTAA ATTGGAGAAA 
ATGCTGAATA ACCAGTATAA TCATGACTTT AATGAAAGAG TCACAGAAGA GAAAGGGCTA 
TCAAGGGAAG ACAAGAAGTT CATGGATATT GTGGAACGAT CTGTAAGACT TAAAGACAAC 
CATTATCAAA TTAAACTGCC TCTTAAGAAA GACGTGCATC TGCCCAATAA CTTTGCTGTG 
GCAAAACAAA GGCTAACAAG TCTAAAACGA AAGTTTCTGC AGAATGAACA TTTCTTCAAA 
GAGTACCGCA ATAAAGTCAA CGACATGTTA CTCAATGGTT TTGCAGAGAA AGTACCTACC 
CAGCAGTTAA GTGGCAGCAG TGGTAAAGTG TGGTACATTC CTCACCACGG AATATATCAT 
CCCAGGAAGA AAAGTCTGCG TGTGGTATTT GACTGTTCTG CATCATTTAA AGGATCATCA 
TTGAATGAAC AGCTCCTGCA AGGTCCCAAC TTTAATAGCA CACTGATTGG AGTCCTCCTG 
AGGTTTCGGC AAGAACCTGT GGCATTCATG GGGGATGTGC AAGCAATGTA CTATCAAGTT 
AAGGTTCCAG AGGAGGATAG AGACCTTCTG CGGTTCTTGT GGTGGCCTGA GGGAGATCTC 
ACGAAAGATG CGTCAGAATT CCGCATGACT GTCCATTTAT TTGGTGCGGT ATCATCTCCA 
AGCTGTGCCA TCTTTGCTCT GAGGAAAACA GCTGATGATA ATCAGGCTGA ATTTCCAGCA 
GAAATTCCAC AAACTGTCAA AGAAAACTTC TATGTCGACG ACTGCTTGAA GAGTGTAGAA 
TCTGAGAAGA AAGCTGTAAC AATGATCAAA CACCTGACCA CGCTCTGCCA AAAGGGAGGA 
TTTACCTTGA CAAAGTGGGT AAGCAACAGT TGCTATGTAC TGCAAGCCAT GCCACAGGAA 
TATAGAGCTA AGGGTGTGGA AGAACTTGAC ATGGACCGAG ATGAACTTCC CATGGAAAGA 
GCCCTTGGAT TACAGTGGTG TGTTCAGAAG GACACATTTA CATTCAAAAT GGCACCTTGT 
CAACGGTCCT GTACTAGACG TGGAATGCTA TCTGTCTCCA GTTCAGTGTA TGACCCACTT 
GGTTTCCTGG CACCAATTCT ACTACCTGCA AAGATAATGC TGCAAGAATT GTGCAGAAGA 
AACTATGGAT GGGATGAAAC GGTACCTCAA GAGCTACTAC ACCGCTGGAC AAGATGGATA 
GAGGAGTTGG AAATGCTGTC TAACTTTGAG ATTAATAGAT GCATCAAGCC CAAAGACTTT 
GGAACTGCCA TACATGCTGA GCTTCATCAT TTCTCAGATG CTAGCGAACT AGGATATGGG 
GCAGTTACCT ATCTCAGGAT CCAAAATAGC AAAAACGTCA TCCACGTTGC ATTTGTGATG 
GGCAAAGCCA GAGTTACACC TTTAAAGACT GTTACCATTC CCCGTCTAGA GTTAACAGCT 
GCAGTTCTTG CCGTTCGTAT GGATCTGATG CTGAAAAAAG AGCTGCAAAT CCCACTGGCA 
GAATCAATAT TCTGGACAGA CAGCACATCA GTGCTTAAGT ACATAAATAA TGAAGACAGA 
CGTTTTCATA CGTTCGTGGC TAATAGGGTC ACCATTATAA GAGAAGCAAC AACAACTTTA 
CAGTGGCGGT ACATTCGTTC CAAGGAAAAC CCAGCAGATG ATGCATCTAG AGGTCTGAGG 
GTGGGTGACT TCATTGAACA CAATAGGTGG ATAGTAGGTC CAAGTTTCTT GTATAAGTCA 
GAAGAGGATT GGCCAGCTGA TAGAGCTGAC ATGGTGATCA AAATTGATGA TCCGGAAATA 
AAAAGAGAAG TTTTAGTTAA CGCAGTAAGT GTAACTGACT CCTCAGATAG CACCACTCGT 
TTAATTGCGC ATTTCTCAGA TTGGAAGAGA TTGAGGGTAG CCGTAGCTTG GTTTCTTAAG 
TATAAGAGAA TGCTTCAGGA ACGGTCTCAC AAGAGGAAAG AGTCTAAGAC TTCTTATGTG 
GGTATGCAGA GCGTTCCAGT GCATGAAAGG TGCACTTTGT CCATTGAAGA CATAAGTGAG 
GCAGAGCTTG CCATTATACG CTTTTGCCAA CAGCAACGAT TTAGTGAGGA AATCGCTACC 
TTGTCAGCTG GAAAAGGAAC TGTGAGTAAG CAAAGCCCTC TGTACAAACT GGATCCACGT 
TTGGATAACG GACTTCTAAG AGTCGGTGGG CGACTAGCCA GAGGTTCACT ACCAGAGGAA 
GCTAAGCATC CACTGATTTT GTCCAAAGAT CAACATATAG CTACTCTCCT CCTGCGAAAT 
ATTCATGAAC GGCTCGGACA TAGTGGTCGA AACCACATAC TGTCCACATT ACGAAGGAAA 
TATTGGATCA CAGGTGCTAA TTCGGCAGTA AGAAAGATAA TGTCTGACTG TTGTCTCTGC 
AGAAGACAAA ATGGTCGAAT GATGCTACAG AAGATGGCAG ACTTGCCCAT GGAGAGGATT 
CTTCCGGACA AACCACCATT CACCAACACT GGGGTTGACT ACTTTGGTCC TATAGAGGTG 
AAGAAAGGAC GAGGCACTGC CAAGCGATAT GGAGTGATAT TTACTTGCCT GTCTAGTAGA 
GCAGTTCACC TTGAAATAGC CAACTCGTTG AATACAGACG CATGCATTAA TGCAATACGT 
CGGTTTGTCT GTAGAAGAGG TCAAGTTGTT AATTTACTGT CTGACAATGG TACCAATTTT 
AAAGGAGCAG AGAAGGAACT CAGAGAGGCA CTCCTAACCT TGAACCAGAC AAATATTGGA 
GGAATGCTAC AACAAAAGGG CATCAAATGG AGTTTCAATC CTCCTTCAGC GTCGCACTAT 
GGTGGTGTTT GGGAGCGTAT GATTCGCATG GTGAGAAGAA TTCTCTCTTC AGTCCTTCAT 
CAACAAAAAT TAGACGATGA TGGACTCCAC ACAGTAATAT GCGAAGTTGA AGCCATCTTA 
AACGACCGCC CCATCACCAA GTTATCTGAC GATCCAAATG ATCTAGAACC CCTGACACCT 
AATCATATCC TCCTCATGAA AGGTAAGCCG TCTTTGCCAC CAGGGCTGTT TGAACATCAT 
GATCTGTACA TCAGAAAGCG ATGGAGGCAA GTTCAGTACA TATCTGATCT CTTTTGGAAA 
CGGTGGATTC GTGAATACCT GCCATTATTG CAAGAGAGGC AGAAATGGAA CGAACAGAGA 
AAAAATCTCA AACCAGGAGA TATCGTGATC ATTATGGATC CTACAGCACC ACGAAGCTCA 
TGGCCTCTTG CAAGAGTGCT GGAAGCATTT CAAGACAAGA AAAAGATTGT GCGTTCAGTG 
CGGCTTCAGA CCAAAAGTAG TATAATAGAA CGGCCTGTGA CTAAACTCTG CCTATTGCAA 
GAATCAGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207691.1
CDS	962..7093
Translation	METNETIDRPAEPSVEINTIEHDNDAQTSKEKRVVKLTAKALADKLGRLQNERKTKLNKAASLRNVLNGLIEKGNVVQAQCVFDDFIKVCDESKGVHECLMGMLPDTEQEKHDVWFKAKMIANNELISKVQLWLSNSGQNVSVGPTGSQIGDGINPEDSVSNATSKRSGKSAGSKSSTTSSVRIKAQAEKAALLARIAALKEKHALEEQEQQIKRKLEQQELDTMLAETAARLSVLEASDNFCSENASDTMNSYLTRETQSLAPPHKLDPMASEFKCAPQSKPQQTQEWTSSSTTPPLLAGALHQASQQPTTRPKEWSDLNKQTTEINRNLHQQSTQLNVPNTNQRYTGGSSSGVHWRNNTTSLVQQQHLISLPARNIPLFDGDPLQYVSFMRAFEQGVEEKASKSDCLYYLEQFTRGQPRELVRSCLYMTPELGYDKAKQLVQEHFGCKYKIAVAYIEKALSWAPIKNEDVNALQAYSLFLRGCCNVMEELQYMHELDMPSNIRAIVLKLPFKLRDQWRTAAHDLQETTGNRALVNDLVRVIERHVQILSDPLYGNISDSPSATTGQRAVNRPRVSPEQRTKGKSFATTITPVKVECTTGSRTISPRHNSASALFCAYCSQSHLLEHCQQFKCKKHRDKINFLKEKHLCFGCLSTSHMSRDCEKRLTCKICSQSHPTVLHINKSDTASMHKAHVAKVTVSTSTQIDKDITSHSFASTAETCGHTGAGADQCLLSIVPVQVKSIKSDQVILTYAFLDPGSSATFCSEQLMQKLHLTGKRTQFLLCTMGQERVVPAYSLTGLEVSGINGNVFHELPQTLTQKKMPVNTDNIVTAETLVKWPYLVGLTPPKIEANVDLLIGSNAPRLLEPWEVINSHGNGPYAIRTVLGWVINGLVHGEDSCLNAECRSAVVNRVSVCKLEKMLNNQYNHDFNERVTEEKGLSREDKKFMDIVERSVRLKDNHYQIKLPLKKDVHLPNNFAVAKQRLTSLKRKFLQNEHFFKEYRNKVNDMLLNGFAEKVPTQQLSGSSGKVWYIPHHGIYHPRKKSLRVVFDCSASFKGSSLNEQLLQGPNFNSTLIGVLLRFRQEPVAFMGDVQAMYYQVKVPEEDRDLLRFLWWPEGDLTKDASEFRMTVHLFGAVSSPSCAIFALRKTADDNQAEFPAEIPQTVKENFYVDDCLKSVESEKKAVTMIKHLTTLCQKGGFTLTKWVSNSCYVLQAMPQEYRAKGVEELDMDRDELPMERALGLQWCVQKDTFTFKMAPCQRSCTRRGMLSVSSSVYDPLGFLAPILLPAKIMLQELCRRNYGWDETVPQELLHRWTRWIEELEMLSNFEINRCIKPKDFGTAIHAELHHFSDASELGYGAVTYLRIQNSKNVIHVAFVMGKARVTPLKTVTIPRLELTAAVLAVRMDLMLKKELQIPLAESIFWTDSTSVLKYINNEDRRFHTFVANRVTIIREATTTLQWRYIRSKENPADDASRGLRVGDFIEHNRWIVGPSFLYKSEEDWPADRADMVIKIDDPEIKREVLVNAVSVTDSSDSTTRLIAHFSDWKRLRVAVAWFLKYKRMLQERSHKRKESKTSYVGMQSVPVHERCTLSIEDISEAELAIIRFCQQQRFSEEIATLSAGKGTVSKQSPLYKLDPRLDNGLLRVGGRLARGSLPEEAKHPLILSKDQHIATLLLRNIHERLGHSGRNHILSTLRRKYWITGANSAVRKIMSDCCLCRRQNGRMMLQKMADLPMERILPDKPPFTNTGVDYFGPIEVKKGRGTAKRYGVIFTCLSSRAVHLEIANSLNTDACINAIRRFVCRRGQVVNLLSDNGTNFKGAEKELREALLTLNQTNIGGMLQQKGIKWSFNPPSASHYGGVWERMIRMVRRILSSVLHQQKLDDDGLHTVICEVEAILNDRPITKLSDDPNDLEPLTPNHILLMKGKPSLPPGLFEHHDLYIRKRWRQVQYISDLFWKRWIREYLPLLQERQKWNEQRKNLKPGDIVIIMDPTAPRSSWPLARVLEAFQDKKKIVRSVRLQTKSSIIERPVTKLCLLQESV

Target ORF information:

RefSeq Version	XM_017352202.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio OTU domain containing 6B (otud6b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352202.1

ORF Insert Sequence:

ATGGAGACAA ATGAAACGAT CGACAGGCCT GCCGAGCCGT CCGTAGAAAT AAACACTATC 
GAACATGATA ATGATGCGCA AACATCAAAA GAAAAACGCG TTGTTAAACT GACTGCTAAA 
GCGCTCGCTG ATAAACTGGG ACGTTTGCAA AATGAAAGGA AAACTAAGCT GAATAAGGCA 
GCCAGTTTAA GAAATGTGTT GAATGGTTTG ATTGAAAAGG GAAATGTTGT ACAAGCTCAA 
TGTGTATTTG ATGATTTTAT TAAGGTCTGT GATGAATCAA AAGGTGTTCA TGAGTGTTTG 
ATGGGTATGT TACCTGATAC TGAACAAGAA AAACATGATG TGTGGTTTAA AGCCAAAATG 
ATCGCCAATA ATGAGCTCAT TTCTAAGGTG CAATTGTGGT TATCAAATTC TGGTCAAAAT 
GTGAGTGTTG GTCCTACTGG TAGCCAAATT GGTGATGGTA TTAATCCAGA GGATAGTGTT 
TCAAATGCAA CTAGTAAACG CTCAGGGAAA AGTGCAGGTA GTAAATCAAG CACTACTTCT 
TCTGTGCGAA TTAAAGCACA AGCGGAAAAG GCTGCATTGC TTGCTCGTAT AGCAGCTTTA 
AAGGAAAAGC ATGCATTGGA AGAGCAAGAG CAGCAGATAA AGAGAAAATT AGAGCAACAG 
GAGTTGGATA CTATGTTGGC TGAAACTGCT GCCAGACTGT CCGTGCTCGA AGCTTCTGAT 
AATTTCTGCA GTGAAAATGC ATCTGATACT ATGAATTCTT ACTTGACGAG AGAAACACAA 
AGTTTAGCAC CTCCACACAA ACTGGACCCG ATGGCAAGTG AATTTAAATG TGCGCCTCAA 
AGTAAACCAC AACAAACACA GGAGTGGACA TCATCATCAA CAACCCCCCC ACTTTTAGCC 
GGAGCGCTAC ATCAAGCTAG TCAACAGCCC ACCACTAGAC CAAAGGAGTG GTCTGATTTA 
AATAAACAAA CAACTGAAAT CAACCGAAAT TTACATCAGC AATCAACCCA ACTTAATGTG 
CCAAATACAA ATCAAAGGTA TACAGGAGGA TCTAGTTCAG GGGTGCACTG GAGGAATAAT 
ACAACATCAC TTGTTCAACA ACAGCATTTG ATATCCTTGC CAGCAAGAAA TATCCCTTTG 
TTCGATGGAG ATCCTCTACA ATATGTCTCC TTCATGAGAG CTTTTGAACA AGGAGTGGAA 
GAAAAGGCTA GTAAGAGCGA CTGTTTGTAC TATCTGGAAC AGTTCACTAG GGGGCAACCA 
AGAGAGCTTG TTCGCAGCTG TTTGTATATG ACGCCTGAGC TAGGTTATGA TAAAGCAAAG 
CAGCTGGTAC AGGAACACTT TGGCTGTAAG TACAAGATTG CTGTGGCGTA CATTGAAAAG 
GCTTTATCTT GGGCTCCAAT AAAGAATGAA GATGTTAATG CTCTACAGGC TTACTCTCTC 
TTTCTGCGAG GCTGCTGCAA TGTTATGGAG GAACTTCAGT ATATGCATGA GCTGGATATG 
CCAAGTAACA TAAGGGCAAT TGTCTTAAAG CTGCCATTTA AACTTCGAGA TCAATGGAGG 
ACTGCTGCAC ATGACTTACA GGAAACCACA GGAAACAGAG CCTTGGTCAA TGACCTGGTG 
AGAGTTATAG AAAGACATGT TCAAATTCTG TCTGATCCAT TGTATGGCAA TATTAGCGAC 
TCACCATCTG CAACAACTGG TCAAAGAGCT GTCAATAGGC CACGAGTATC TCCAGAACAA 
AGAACTAAAG GAAAAAGCTT TGCTACTACC ATTACACCTG TGAAAGTTGA GTGTACCACA 
GGATCAAGGA CCATCTCACC TAGACATAAC TCTGCATCTG CTTTATTCTG TGCATACTGT 
TCTCAGAGTC ATCTATTAGA ACATTGTCAA CAGTTCAAAT GCAAAAAACA CAGAGACAAG 
ATTAATTTTC TGAAGGAGAA ACACCTCTGC TTTGGTTGTT TGAGTACTTC ACACATGAGT 
CGTGACTGTG AGAAACGCCT AACTTGTAAA ATATGCAGCC AAAGCCATCC TACTGTACTT 
CATATTAACA AGAGCGACAC AGCTAGCATG CATAAAGCGC ATGTTGCTAA GGTCACAGTA 
AGTACTTCTA CTCAAATTGA CAAAGATATT ACATCACACT CATTCGCTTC AACTGCTGAG 
ACTTGTGGAC ATACAGGGGC CGGAGCAGAT CAGTGTCTGT TATCCATTGT GCCAGTCCAA 
GTCAAGTCCA TCAAATCTGA TCAGGTAATA CTGACATATG CATTCTTGGA TCCTGGCAGC 
TCCGCCACCT TCTGCTCAGA ACAGCTTATG CAAAAGCTTC ATCTTACTGG GAAACGAACC 
CAGTTCCTCT TATGCACTAT GGGGCAAGAA AGGGTGGTGC CAGCATATTC ACTCACAGGA 
CTGGAAGTGT CTGGTATTAA TGGTAATGTC TTCCATGAAC TTCCTCAAAC CCTAACACAA 
AAGAAAATGC CCGTAAACAC TGATAACATT GTTACTGCTG AAACTCTGGT AAAATGGCCA 
TACCTGGTAG GGTTAACACC TCCCAAGATA GAAGCTAACG TAGACCTGTT GATTGGAAGC 
AACGCACCAA GGTTGTTGGA ACCTTGGGAG GTTATTAATA GCCATGGGAA TGGCCCATAT 
GCTATAAGGA CCGTGTTGGG ATGGGTCATT AATGGTCTTG TGCATGGAGA AGACTCCTGT 
CTTAATGCTG AATGTAGGTC AGCTGTAGTT AACAGGGTTT CAGTATGTAA ATTGGAGAAA 
ATGCTGAATA ACCAGTATAA TCATGACTTT AATGAAAGAG TCACAGAAGA GAAAGGGCTA 
TCAAGGGAAG ACAAGAAGTT CATGGATATT GTGGAACGAT CTGTAAGACT TAAAGACAAC 
CATTATCAAA TTAAACTGCC TCTTAAGAAA GACGTGCATC TGCCCAATAA CTTTGCTGTG 
GCAAAACAAA GGCTAACAAG TCTAAAACGA AAGTTTCTGC AGAATGAACA TTTCTTCAAA 
GAGTACCGCA ATAAAGTCAA CGACATGTTA CTCAATGGTT TTGCAGAGAA AGTACCTACC 
CAGCAGTTAA GTGGCAGCAG TGGTAAAGTG TGGTACATTC CTCACCACGG AATATATCAT 
CCCAGGAAGA AAAGTCTGCG TGTGGTATTT GACTGTTCTG CATCATTTAA AGGATCATCA 
TTGAATGAAC AGCTCCTGCA AGGTCCCAAC TTTAATAGCA CACTGATTGG AGTCCTCCTG 
AGGTTTCGGC AAGAACCTGT GGCATTCATG GGGGATGTGC AAGCAATGTA CTATCAAGTT 
AAGGTTCCAG AGGAGGATAG AGACCTTCTG CGGTTCTTGT GGTGGCCTGA GGGAGATCTC 
ACGAAAGATG CGTCAGAATT CCGCATGACT GTCCATTTAT TTGGTGCGGT ATCATCTCCA 
AGCTGTGCCA TCTTTGCTCT GAGGAAAACA GCTGATGATA ATCAGGCTGA ATTTCCAGCA 
GAAATTCCAC AAACTGTCAA AGAAAACTTC TATGTCGACG ACTGCTTGAA GAGTGTAGAA 
TCTGAGAAGA AAGCTGTAAC AATGATCAAA CACCTGACCA CGCTCTGCCA AAAGGGAGGA 
TTTACCTTGA CAAAGTGGGT AAGCAACAGT TGCTATGTAC TGCAAGCCAT GCCACAGGAA 
TATAGAGCTA AGGGTGTGGA AGAACTTGAC ATGGACCGAG ATGAACTTCC CATGGAAAGA 
GCCCTTGGAT TACAGTGGTG TGTTCAGAAG GACACATTTA CATTCAAAAT GGCACCTTGT 
CAACGGTCCT GTACTAGACG TGGAATGCTA TCTGTCTCCA GTTCAGTGTA TGACCCACTT 
GGTTTCCTGG CACCAATTCT ACTACCTGCA AAGATAATGC TGCAAGAATT GTGCAGAAGA 
AACTATGGAT GGGATGAAAC GGTACCTCAA GAGCTACTAC ACCGCTGGAC AAGATGGATA 
GAGGAGTTGG AAATGCTGTC TAACTTTGAG ATTAATAGAT GCATCAAGCC CAAAGACTTT 
GGAACTGCCA TACATGCTGA GCTTCATCAT TTCTCAGATG CTAGCGAACT AGGATATGGG 
GCAGTTACCT ATCTCAGGAT CCAAAATAGC AAAAACGTCA TCCACGTTGC ATTTGTGATG 
GGCAAAGCCA GAGTTACACC TTTAAAGACT GTTACCATTC CCCGTCTAGA GTTAACAGCT 
GCAGTTCTTG CCGTTCGTAT GGATCTGATG CTGAAAAAAG AGCTGCAAAT CCCACTGGCA 
GAATCAATAT TCTGGACAGA CAGCACATCA GTGCTTAAGT ACATAAATAA TGAAGACAGA 
CGTTTTCATA CGTTCGTGGC TAATAGGGTC ACCATTATAA GAGAAGCAAC AACAACTTTA 
CAGTGGCGGT ACATTCGTTC CAAGGAAAAC CCAGCAGATG ATGCATCTAG AGGTCTGAGG 
GTGGGTGACT TCATTGAACA CAATAGGTGG ATAGTAGGTC CAAGTTTCTT GTATAAGTCA 
GAAGAGGATT GGCCAGCTGA TAGAGCTGAC ATGGTGATCA AAATTGATGA TCCGGAAATA 
AAAAGAGAAG TTTTAGTTAA CGCAGTAAGT GTAACTGACT CCTCAGATAG CACCACTCGT 
TTAATTGCGC ATTTCTCAGA TTGGAAGAGA TTGAGGGTAG CCGTAGCTTG GTTTCTTAAG 
TATAAGAGAA TGCTTCAGGA ACGGTCTCAC AAGAGGAAAG AGTCTAAGAC TTCTTATGTG 
GGTATGCAGA GCGTTCCAGT GCATGAAAGG TGCACTTTGT CCATTGAAGA CATAAGTGAG 
GCAGAGCTTG CCATTATACG CTTTTGCCAA CAGCAACGAT TTAGTGAGGA AATCGCTACC 
TTGTCAGCTG GAAAAGGAAC TGTGAGTAAG CAAAGCCCTC TGTACAAACT GGATCCACGT 
TTGGATAACG GACTTCTAAG AGTCGGTGGG CGACTAGCCA GAGGTTCACT ACCAGAGGAA 
GCTAAGCATC CACTGATTTT GTCCAAAGAT CAACATATAG CTACTCTCCT CCTGCGAAAT 
ATTCATGAAC GGCTCGGACA TAGTGGTCGA AACCACATAC TGTCCACATT ACGAAGGAAA 
TATTGGATCA CAGGTGCTAA TTCGGCAGTA AGAAAGATAA TGTCTGACTG TTGTCTCTGC 
AGAAGACAAA ATGGTCGAAT GATGCTACAG AAGATGGCAG ACTTGCCCAT GGAGAGGATT 
CTTCCGGACA AACCACCATT CACCAACACT GGGGTTGACT ACTTTGGTCC TATAGAGGTG 
AAGAAAGGAC GAGGCACTGC CAAGCGATAT GGAGTGATAT TTACTTGCCT GTCTAGTAGA 
GCAGTTCACC TTGAAATAGC CAACTCGTTG AATACAGACG CATGCATTAA TGCAATACGT 
CGGTTTGTCT GTAGAAGAGG TCAAGTTGTT AATTTACTGT CTGACAATGG TACCAATTTT 
AAAGGAGCAG AGAAGGAACT CAGAGAGGCA CTCCTAACCT TGAACCAGAC AAATATTGGA 
GGAATGCTAC AACAAAAGGG CATCAAATGG AGTTTCAATC CTCCTTCAGC GTCGCACTAT 
GGTGGTGTTT GGGAGCGTAT GATTCGCATG GTGAGAAGAA TTCTCTCTTC AGTCCTTCAT 
CAACAAAAAT TAGACGATGA TGGACTCCAC ACAGTAATAT GCGAAGTTGA AGCCATCTTA 
AACGACCGCC CCATCACCAA GTTATCTGAC GATCCAAATG ATCTAGAACC CCTGACACCT 
AATCATATCC TCCTCATGAA AGGTAAGCCG TCTTTGCCAC CAGGGCTGTT TGAACATCAT 
GATCTGTACA TCAGAAAGCG ATGGAGGCAA GTTCAGTACA TATCTGATCT CTTTTGGAAA 
CGGTGGATTC GTGAATACCT GCCATTATTG CAAGAGAGGC AGAAATGGAA CGAACAGAGA 
AAAAATCTCA AACCAGGAGA TATCGTGATC ATTATGGATC CTACAGCACC ACGAAGCTCA 
TGGCCTCTTG CAAGAGTGCT GGAAGCATTT CAAGACAAGA AAAAGATTGT GCGTTCAGTG 
CGGCTTCAGA CCAAAAGTAG TATAATAGAA CGGCCTGTGA CTAAACTCTG CCTATTGCAA 
GAATCAGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ

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otud6b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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