ankrd12 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ankrd12
Entrez Gene ID	393119
Full Name	ankyrin repeat domain 12
Synonyms	wu:fi16c02,zgc:55925
General protein information	Preferred Names ankyrin repeat domain 12 Names ankyrin repeat domain-containing protein 12 fi16c02
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357915.1	XP_017213404.1	ankyrin repeat domain-containing protein 12 isoform X1
XM_017357916.1	XP_017213405.1	ankyrin repeat domain-containing protein 12 isoform X2
XM_017357916.2	XP_017213405.1	ankyrin repeat domain-containing protein 12 isoform X2
NM_200150.1	NP_956444.1	ankyrin repeat domain-containing protein 12

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005648	XP_315665.3
Danio rerio (zebrafish)	ankrd12	NP_956444.1
Rattus norvegicus (Norway rat)	LOC100910483	XP_003750800.2

Gene Ontology
Bibliography

Related articles in PubMed

Analyses of mRNA structure dynamics identify embryonic gene regulatory programs.
Beaudoin JD, Novoa EM, Vejnar CE, Yartseva V, Takacs CM, Kellis M, Giraldez AJ
Nature structural & molecular biology25(8)677-686(2018 08)

Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish.
Grajevskaja V, Camerota D, Bellipanni G, Balciuniene J, Balciunas D
PloS one13(6)e0197293(2018)

4.1Ba is necessary for glutamatergic synapse formation in the sensorimotor circuit of developing zebrafish.
Fierro J Jr, Haynes DR, Washbourne P
PloS one13(10)e0205255(2018)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ankrd12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankrd12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35058	NM_200150.1 Latest version!	Danio rerio ankyrin repeat domain 12 (ankrd12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa56269	XM_017357915.1 Latest version!	Danio rerio ankyrin repeat domain 12 (ankrd12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa56270	XM_017357916.2 Latest version!	Danio rerio ankyrin repeat domain 12 (ankrd12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa56270	XM_017357916.1	Danio rerio ankyrin repeat domain 12 (ankrd12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ankrd12 ( NM_200150.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956444.1 Danio rerio ankyrin repeat domain 12 (ankrd12), mRNA.

Price & Availability↑

CloneID	ODa35058
Clone ID Related Accession (Same CDS sequence)	NM_200150.1
Accession Version	NM_200150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat domain-containing protein 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044542.1. On Jul 6, 2005 this sequence version replaced XM_697133.1. ##Evidence-Data-START## Transcript exon combination :: BC044542.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAAC CGGGATCCGA CCGAGATGAA GCCATGGTGG AGAAGACAGC GGGGAAGAAG 
AGTAAAGAGA AAATCTCTCC GTTTGCTAAA ACTCCGAAGC TGGATCGGAG TGAACTCTTG 
GGGAAAGAGG GGAAGTCGAA ATCCTCCATG AAGCGGAAAC TCTCTTTCAC TGTCAGTCCA 
TCCCGAAACG AGGAGCGCGA CTCGGATGCA GATGACTCTG ACCCGGGGCA GTCGAGCGAT 
GCGTGGGGAG AGAGATTACT TCCTCCCTGC AGGATATACG CAGACAAAGA TGGACCGGAT 
AAGAAGAAGG TTAAGAAGGA GTCGGGGGGT AAGAAGTCGA CACCAGTGAA TATTTTGTTC 
GGGTACCCTT TATCAGAACG AAAGCAGATG GCACTTGTCA TGCAGATGAC GGCGAGAGAC 
AACAGTCCAG ATACCACACC GAACCACCCG TCTCAATCTC CGGCGGTGCA GAAGAAGATC 
GCCAGCAGCT CAACCTCACG ACAAAAAGAC AAAGTGAACA AGCGCAACGA GAGAGGAGAG 
ACGCCTCTGC ATATGGCCGC CATCAGAGGA GACGTCAAAC AAGTGAAGGA GCTCATCGGC 
CTCGGAGCGG ATGTAAACGT CAAAGACTTT GCAGGTTGGA CGCCTCTCCA TGAAGCCTGC 
AACCTTGGTT ACTATGATGT AGCCAAGGTT TTGATTGGTG CAGGAGCGGA AGTGAACACG 
CAGGGATTGG ACGATGACAC GCCGCTTCAT GACGCCTCAA GCAGTGGACA CAAAGACATT 
GTGAAGCTGC TCTTGCGTCA TGGAGGCAAT GCTTTCCAAG CCAACAAGAG AGGGGAGAGA 
CCGGTTGATG TTGCTGACTC TCAGGAGCTG GAGCAGCTAC TCAAGGGAGA AATCCCACTG 
TCAGAACCAG AAGACAGCTC TTCAGAATCA GAGGACCCAT CTTCAGTGAA TCCATCCAGT 
GTAGACGAGA ACCTGGAGTT TTCTGATGCT GAAAAAGACT CTGACTGTAA ATCGACAACT 
GTCAAAGCCT CATCGTCCAT GTCAGGGCTG GATGAATATG AGTTTAAAGA TGAGGAAGAG 
GAAGAGGACC TCAGTAAGTC ACTTAATGAG CGACACCTCT TTAGGCGAGA GCTACGGCAA 
AAGGAGAAGG AGGAAAAGGA CAGAAACCAC TATGCTTCAA AACAAGGTAG CAAAAGCGAC 
CAGTCCATGC TGGCTTGCAA GTCTAAAAAA ACAAAGGCGT CCCCGTGTCT ACTGCAGTTC 
AGACAGTTCA AGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956444.1
CDS	206..1483
Translation	MAKPGSDRDEAMVEKTAGKKSKEKISPFAKTPKLDRSELLGKEGKSKSSMKRKLSFTVSPSRNEERDSDADDSDPGQSSDAWGERLLPPCRIYADKDGPDKKKVKKESGGKKSTPVNILFGYPLSERKQMALVMQMTARDNSPDTTPNHPSQSPAVQKKIASSSTSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELIGLGADVNVKDFAGWTPLHEACNLGYYDVAKVLIGAGAEVNTQGLDDDTPLHDASSSGHKDIVKLLLRHGGNAFQANKRGERPVDVADSQELEQLLKGEIPLSEPEDSSSESEDPSSVNPSSVDENLEFSDAEKDSDCKSTTVKASSSMSGLDEYEFKDEEEEEDLSKSLNERHLFRRELRQKEKEEKDRNHYASKQGSKSDQSMLACKSKKTKASPCLLQFRQFKR

Target ORF information:

RefSeq Version	NM_200150.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat domain 12 (ankrd12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200150.1

ORF Insert Sequence:

ATGGCCAAAC CGGGATCCGA CCGAGATGAA GCCATGGTGG AGAAGACAGC GGGGAAGAAG 
AGTAAAGAGA AAATCTCTCC GTTTGCTAAA ACTCCGAAGC TGGATCGGAG TGAACTCTTG 
GGGAAAGAGG GGAAGTCGAA ATCCTCCATG AAGCGGAAAC TCTCTTTCAC TGTCAGTCCA 
TCCCGAAACG AGGAGCGCGA CTCGGATGCA GATGACTCTG ACCCGGGGCA GTCGAGCGAT 
GCGTGGGGAG AGAGATTACT TCCTCCCTGC AGGATATACG CAGACAAAGA TGGACCGGAT 
AAGAAGAAGG TTAAGAAGGA GTCGGGGGGT AAGAAGTCGA CACCAGTGAA TATTTTGTTC 
GGGTACCCTT TATCAGAACG AAAGCAGATG GCACTTGTCA TGCAGATGAC GGCGAGAGAC 
AACAGTCCAG ATACCACACC GAACCACCCG TCTCAATCTC CGGCGGTGCA GAAGAAGATC 
GCCAGCAGCT CAACCTCACG ACAAAAAGAC AAAGTGAACA AGCGCAACGA GAGAGGAGAG 
ACGCCTCTGC ATATGGCCGC CATCAGAGGA GACGTCAAAC AAGTGAAGGA GCTCATCGGC 
CTCGGAGCGG ATGTAAACGT CAAAGACTTT GCAGGTTGGA CGCCTCTCCA TGAAGCCTGC 
AACCTTGGTT ACTATGATGT AGCCAAGGTT TTGATTGGTG CAGGAGCGGA AGTGAACACG 
CAGGGATTGG ACGATGACAC GCCGCTTCAT GACGCCTCAA GCAGTGGACA CAAAGACATT 
GTGAAGCTGC TCTTGCGTCA TGGAGGCAAT GCTTTCCAAG CCAACAAGAG AGGGGAGAGA 
CCGGTTGATG TTGCTGACTC TCAGGAGCTG GAGCAGCTAC TCAAGGGAGA AATCCCACTG 
TCAGAACCAG AAGACAGCTC TTCAGAATCA GAGGACCCAT CTTCAGTGAA TCCATCCAGT 
GTAGACGAGA ACCTGGAGTT TTCTGATGCT GAAAAAGACT CTGACTGTAA ATCGACAACT 
GTCAAAGCCT CATCGTCCAT GTCAGGGCTG GATGAATATG AGTTTAAAGA TGAGGAAGAG 
GAAGAGGACC TCAGTAAGTC ACTTAATGAG CGACACCTCT TTAGGCGAGA GCTACGGCAA 
AAGGAGAAGG AGGAAAAGGA CAGAAACCAC TATGCTTCAA AACAAGGTAG CAAAAGCGAC 
CAGTCCATGC TGGCTTGCAA GTCTAAAAAA ACAAAGGCGT CCCCGTGTCT ACTGCAGTTC 
AGACAGTTCA AGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankrd12 ( XM_017357915.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213404.1 Danio rerio ankyrin repeat domain 12 (ankrd12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56269
Clone ID Related Accession (Same CDS sequence)	XM_017357915.1
Accession Version	XM_017357915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CGGGATCCGA CCGAGATGAA GCCATGGTGG AGAAGACAGC GGGGAAGAAG 
AGTAAAGAGA AAATCTCTCC GTTTGCTAAA ACTCCGAAGC TGGATCGGAG TGAACTCTTG 
GGGAAAGAGG GGAAGTCGAA ATCCTCCATG AAGCGGAAAC TCTCCTTCAC TGTCAGTCCA 
TCCCGAAACG AGGAGCGCGA CTCGGATGCA GATGACTCTG ACCCGGGGCA GTCGAGCGAT 
GCGTGGGGAG AGAGATTACT TCCTCCCTGC AGGATATACG CAGACAAAGA TGGACCGGAT 
AAGAAGAAGG TTAAGAAGGA GTCGGGGGGT AAGAAGTCGA CACCAGTGAA TATTTTGTTC 
GGGTACCCTT TATCAGAACG AAAGCAGATG GCACTTGTCA TGCAGATGAC GGCGAGAGAC 
AACAGTCCAG ATACCACACC GAACCACCCG TCTCAATCTC CGGCGGTGCA GAAGAAGATC 
GCCAGCAGCT CAACCTCACG ACAAAAAGAC AAAGTGAACA AACGCAACGA GAGAGGAGAG 
ACGCCTCTGC ATATGGCCGC CATCAGAGGA GACGTCAAAC AAGTGAAGGA GCTCATCGGC 
CTCGGAGCGG ATGTAAACGT CAAAGACTTT GCAGGTTGGA CGCCTCTCCA TGAAGCCTGC 
AACCTTGGTT ACTATGATGT AGCCAAGGTT TTGATTGGTG CAGGAGCGGA AGTGAACACG 
CAGGGATTGG ACGATGACAC GCCGCTTCAT GACGCCTCAA GCAGTGGACA CAAAGACATT 
GTGAAGCTGC TCTTGCGTCA TGGAGGCAAT GCTTTCCAAG CCAACAAGAG AGGGGAGAGA 
CCGGTTGATG TTGCTGACTC TCAGGAGCTG GAGCAGCTAC TCAAGGGAGA AATCCCACTG 
TCAGAACCAG AAGACAGCTC TTCAGAATCA GAGGACCCAT CTTCAGTGAA TCCATCCAGT 
GTAGACGAGA ACCTGGAGTT TTCTGATGCT GAAAAAGACT CTGACTGTAA ATCGACAACT 
GTCAAAGCCT CATCGTCCAT GTCAGGGCTG GATGAATATG AGTTTAAAGA TGAGGAAGAG 
GAAGAGGACC TCAGTAAGTC ACTTAATGAG CGACACCTCT TTAGGCGAGA GCTACGGCAA 
AAGGAGAAGG AGGAAAAGGA CAGAAACCAC TATGCTTCAA AACAAGGTAG CAAAAGCGAC 
CAGTCCATGC TGGCTTGCAA GTCTAAAAAA ACAAAGGCGT CCCGTGTCTA CTGCAGTTCA 
GACAGTTCAA GCGATGAAGC AGAGGTGCAG ACGGAAAGAA GGAGCTCACC TACTAGGTCA 
GTCAGTGTGG ATGGACATAA AACGGACAGT AGATCGAAAA AAGAAAGCTT GACTCTAACG 
CCAGTGGAGC AGAAAGAAAA AGGTAAACAG AAGAAAAAGA ACAAGAGCCA AAGCAAAAAC 
AAAGAAAACC AGGAAGTCAG AGAAGAAGGG AAAGAGAACA GCAAATCCCT TCTGTTTTCC 
TCTCCGACTG TGTCCGATAA TTCTGACAAG GGCGTCAGAG AAGAGGACTC TTTCAAAATG 
TCATTCAGTG CAAAGGATGA CACATCGGTT CACCTATTTC ACCTGTCTGT AGTCAAATCC 
CCAAAGCTGA ACCACAGCCA AACTGACAAG CAGGCAACCC CGCTGAAACA GGAAAACGCT 
AAGACATGTG TTTCAATCGC TGATGGATCT TGCCCTGGGG ACGGCATCAA ATATAACCAC 
TACACAGACT CTGACTATTG CACAGAAGAT TCTAGCAGTA AAAGCTGCAA ACATAAGGAA 
AAGAGCAAGC ACCATCACAA GGAGCCTAAC TTGGAAGGAG ATGACGGCAG TTCGAGTCCT 
TTCAAAGACA GCAGTTTAAG CAACAGTATA GACAGCTCTG AGGGTGCGTT TAGGAAGACT 
GACAAAGATG GGAAAGTTGT AAAAAAGCAC AAGCTAAAAC ACAAGGAGAA GGAAAAGGAG 
AAATATAGGA AAGAGTACGA GGCAGAGAGG AATCGCCACC GGCAGAAAGA GCCACGCAAG 
GACGGACATA GAAACCTCGA GTTTGATCGA GAGTTCTGGA AGGAGAACTT CTTCAAAAGT 
GATGAAAATG AGGAACCTGT GGGCAAAGGT GAGATACCTG CTGGTGGATC TCCAGTAAAA 
TCCTCAGATT CGTCACCTGT TAAAGAGGAA AAAGGGGCAT CAAAAGACAA ACATTCAACT 
AGTAGCAGTA ACAAGGACAG GAGGCCAAAA GAGGAAAGAG ATAAAACCAT TAAAAAGGAG 
AAAAAAGAGA TTCCTCTTAA AGAGGAGCGA GGAAAAGAAG ATGAAACAGA TGAGCGGACT 
GATGGTACTT TCTCTGTACG AATTCCTGAT GGCTCACAAC ACTTGTCAGA AGAAGTGGAT 
GAGAAACCAG CAACTTGTAA TTCGGCTGAC CAAGAGCAAC TGGAGTCTCC AGAGAAGAAC 
TCTCGAGAAA AAACTGATAA GCGACCACCA ACAAAGGATC GGGAATCTGA GAAGTCTGAT 
AAGAGACACT CGGATAAAGA TAAGAAGGTA AAATCTGAGC ATTTAGCTGA CAAGCCTGAG 
CTCCACAACT CTACAGATCG ATGGAAGGAA AAGGAGAAAT TAACAAATGT TTCATATTCG 
CCCAATGATA AGAGCCATAA AGAGGGTGAA AAACTCAAAC CAATGACTCC AGTGAAAAAG 
CATGAGGAGA ACAAGAAGAA TAAGGACAAG CTTGATAAAA AAGCGGAGAA AGAGCACGCT 
TCTGGTGACC ACAGAGACAA AGATAAAACA ACTTCTGAAA AGAAAGGAAA AGCTCCTGAG 
AAAACTGCTG ATCATGGAAA ATCTGACCGC AGTAAAGACA AAGAACGGGA CAAAGATTCT 
GACAAGAGGA AAAAAGATAA ATCAACCACT CCATCTTCCT CTTCCTACTC GAATCTAAAG 
TCGTTATTGG AAGAAAAGAA AGGCTATGTT TGTGAGAATA ATAAGGTTGT CTCATCTAAA 
TCAAAGGAAG AGGTAACCAA GCCTTCAGAG AAAGACCGAG ATCGAAGAGA GAGGGACCGG 
GAGTCTGAAA GACATCGAGA TCGGGAACGA GATCGGCACA AGGAAAAGGA CAAAGATCGA 
TCCCAGTTTA ACAGGGATGG CAAAATTAAC AAACCGAAAC CAAGTGAAGT GGACTCCAAA 
TCTAAGGTTA CACCTATTCT AAAGGATACT CGTCCCAAAG AAAAGAGGCT GGTGAATGAT 
GACCTCATGA AGACCAGCTT TGAACGCATG CTCAGTCTCA AAGATCAGGA AATCGAACAG 
TGGCACAAAA AGCACTTGGA GAAAATCAAG CAGAAGGAAC GGGAGCGGCT AAAACAGCGT 
CCTGGAATTG ACCCTGCGAA ACAACAAAAA AACAAGGAAA AAATTAAATC ATCCTCTACG 
TCCACTGAGC CATGTCTAAC CAAGGAACTA ACTCGGTCAA AGAGCTCAGA AGCGCCAGAT 
GGACAACGTG AAAAGATCTT AAAGGACGCC ACTAGTGTGA GAACTCTCTC GCTAGATGCA 
AAAACCTTGG GGAAGAACGG TCCTATAATT GAAAACAATC TAAGTCGATC TCCGAGACCA 
GAAAGTGAAA AATCTGGTCT TATGTCAAGA TCAATATCAA TGATCTCTGT GGCAAGTTCA 
GAAGACTCCT GCCAGGCAAC TATTTTGACA CCTAGACCAA CTGACCAATA TGATTCTGAC 
ATGAACGTGG AAGCCTTAGA TTCGCAGCCA CCTTTACTTC AGTCTTCCCT TGTTGCACAG 
TCCTCCAGGT CGCCTAGTGT TCATGATAAA GATATTAGCA GTCTTCCCGA ATCATCGGCT 
CTCTGCAATC GCACACCATT GGCAAGCAGG CATGCATCAC CATGCTTAAG GGCTATTCTG 
GATGAAGAAG CCAAGGTACC AACAACTGAA ACAAAACCCA CAGAAGAGTC TCCGGTAATC 
AGTATTGCAT CACAACCAAC CAGCGAGCAA TCTCAAGTTC ATCTAACTGA GATTAGTGTC 
CCCCCTGTTC AGGAGACTCG GAGCAGTCAA AGTCTTACAA ACTCTTTTCC AGAAAATGAA 
ATTCTGTCTA GTAATGAAGC CGAAGTCGTC AGTTCCACAT GTCAAGTACA ACCTTCGCAG 
GCTAGTGCTA AAGACAGTGT AACACAAGCA AACAGTGTGT TTCCGAGCCA TTTGGAAGAG 
CAGAACAAGT TTTGCACCAC TAGTAGTCAA ATAGAGCATG CAAGTCTAGG TCAGCTTGAT 
GTCCCTGGTG CAGAGGGAGT AAAAAAGGAG GCTTCACTTG CTGTTGCATC ATTACAGTCA 
TCTCATACAT CAACTTCATC ATCAGATTCC AATGCACATT TGCCTTCGAG TAGTCAGCAT 
TGTGAGATTA GCTCTGCTTC CAGTGGTGAA CAGACAATGA CAACAGACTC TAGTTTGAGA 
ACAGAGCAAA GAGACAAACC ATTGGTTGCT TCAGAGGACA AGATTGGGGA ACATGTCACT 
AAAACTGATT GCGCGAGCAC TCCAAGTCCA TCTACAGCAT CCTGTAGATC CAGCTCTGAG 
GAATCTACTA AACCACTGCT GAAGGCGACC AGTGTTCAGG AAGATACTGA AAAGGGCAAT 
GAACAAGTGC AAGAAGAAAG TATCTCAAGG TTCTCCAATG ACCCATTGGT TGCAGTTGAT 
GCTCAACCAG AGAAAGAAGA GCAAGGCCTA CATTTGGCTT CGGTGACAAA TGTTTCTTGC 
TGTGCCAGTC CAGAAAGAAT CCCAGAGGAG CCCATGGAGG TTCCAGAGGA AGTTTCGGAT 
AAGAGCCGAA TCACAGAAAT TGATGAGGCC AAAGTCTTGT CTTCAGAAGA AGGTGCAGTG 
ACTTCATCTG AAAGTAAGGC TGACTCAGAA ACGCCAGACC CGTTTTCTCT TGACGAGAAG 
CCTTTGAAAT CGGACATTCT GGCTAATGTA GAACAGGCTC AAAGCAGTGC TCCCATCCAC 
CCTGATCATC AGGCAGAGCC AACCAGTGGA GAGACCTCTG GAAGTTCCTC TCCGATATCA 
GTCATGGACA GAGATTCAGA TATATCTGGG GCCAGAGCCA AAGTCAGACT CCTTGAAGAA 
GAAGCTGACA TTCAAGCGAC CCATCCAAGA AAACGAAAAA TGCCTAGAGC TTCTCCGCTA 
GCCCAAGCAA ATCTTACAGC TCAGCAGGTC AGAGAAAAGA CACAGCAGTC CCTCGCTGCG 
ATTGTAGATG CACTGAAGCT TGAGGAGATT CAGCCTTACC AGACAGAGAG AGCCAACCCA 
TATTATGAGT ACTTGCACAT TCGCAAAAAG ATCGAAGAGA AGAGGAAAGT CCTTTGTAGT 
GTCATTCCTC AAGCGCCTCA GTACTACGAC GAGTATGTGA CATTCAATGG GTCCTATCTG 
CTGGATGGAA ACCCTCTCAG CAAACTCTGC ATCCCAACAA TAACGCCTCC ACCCTCTTTA 
CCTGAACCAC TGAAGGAGAT GTTCAAACAG CAAGAGATGG TGCGGATGAA GCTGAGACTG 
CAGCACAGCA TCGAAAGGGA AAAACTGATT GTATCCAATG AGCAGGAGGT TTTGAGAGTT 
CATTACCGCG CTGCGAGAAC ACTAGCCAAT CAGACGCTTC CGTTCAGTGC CTGCACAGTC 
CTATTGGATG CCGAAGTATA CAACATGCCC CAAGAAGTCC AGGGTGATGA TGGGAAGACT 
TCTGTTCGAG ATCGATTCAA TGCGAGGCAG TTCATGTCTT GGCTTCAGGA TGTAGACGAC 
AAGTTTGACA AGTTGAAGAC GTGTCTCCTG ATGCGGCAGC AGCATGAAGC GGCAGCGTTA 
AACGCAGTCC AGCGTCTGGA GTGGCAGCTA AAGCTACAGG AACTGGATCC CGCCACTTAC 
AAGTCCACCA GCATTTTCGA GATTCCCGAA TTCTACATCC CGCTCGTGGA GGTCAACGAC 
GACTTCGACC TGACGCCGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213404.1
CDS	259..6222
Translation	MAKPGSDRDEAMVEKTAGKKSKEKISPFAKTPKLDRSELLGKEGKSKSSMKRKLSFTVSPSRNEERDSDADDSDPGQSSDAWGERLLPPCRIYADKDGPDKKKVKKESGGKKSTPVNILFGYPLSERKQMALVMQMTARDNSPDTTPNHPSQSPAVQKKIASSSTSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELIGLGADVNVKDFAGWTPLHEACNLGYYDVAKVLIGAGAEVNTQGLDDDTPLHDASSSGHKDIVKLLLRHGGNAFQANKRGERPVDVADSQELEQLLKGEIPLSEPEDSSSESEDPSSVNPSSVDENLEFSDAEKDSDCKSTTVKASSSMSGLDEYEFKDEEEEEDLSKSLNERHLFRRELRQKEKEEKDRNHYASKQGSKSDQSMLACKSKKTKASRVYCSSDSSSDEAEVQTERRSSPTRSVSVDGHKTDSRSKKESLTLTPVEQKEKGKQKKKNKSQSKNKENQEVREEGKENSKSLLFSSPTVSDNSDKGVREEDSFKMSFSAKDDTSVHLFHLSVVKSPKLNHSQTDKQATPLKQENAKTCVSIADGSCPGDGIKYNHYTDSDYCTEDSSSKSCKHKEKSKHHHKEPNLEGDDGSSSPFKDSSLSNSIDSSEGAFRKTDKDGKVVKKHKLKHKEKEKEKYRKEYEAERNRHRQKEPRKDGHRNLEFDREFWKENFFKSDENEEPVGKGEIPAGGSPVKSSDSSPVKEEKGASKDKHSTSSSNKDRRPKEERDKTIKKEKKEIPLKEERGKEDETDERTDGTFSVRIPDGSQHLSEEVDEKPATCNSADQEQLESPEKNSREKTDKRPPTKDRESEKSDKRHSDKDKKVKSEHLADKPELHNSTDRWKEKEKLTNVSYSPNDKSHKEGEKLKPMTPVKKHEENKKNKDKLDKKAEKEHASGDHRDKDKTTSEKKGKAPEKTADHGKSDRSKDKERDKDSDKRKKDKSTTPSSSSYSNLKSLLEEKKGYVCENNKVVSSKSKEEVTKPSEKDRDRRERDRESERHRDRERDRHKEKDKDRSQFNRDGKINKPKPSEVDSKSKVTPILKDTRPKEKRLVNDDLMKTSFERMLSLKDQEIEQWHKKHLEKIKQKERERLKQRPGIDPAKQQKNKEKIKSSSTSTEPCLTKELTRSKSSEAPDGQREKILKDATSVRTLSLDAKTLGKNGPIIENNLSRSPRPESEKSGLMSRSISMISVASSEDSCQATILTPRPTDQYDSDMNVEALDSQPPLLQSSLVAQSSRSPSVHDKDISSLPESSALCNRTPLASRHASPCLRAILDEEAKVPTTETKPTEESPVISIASQPTSEQSQVHLTEISVPPVQETRSSQSLTNSFPENEILSSNEAEVVSSTCQVQPSQASAKDSVTQANSVFPSHLEEQNKFCTTSSQIEHASLGQLDVPGAEGVKKEASLAVASLQSSHTSTSSSDSNAHLPSSSQHCEISSASSGEQTMTTDSSLRTEQRDKPLVASEDKIGEHVTKTDCASTPSPSTASCRSSSEESTKPLLKATSVQEDTEKGNEQVQEESISRFSNDPLVAVDAQPEKEEQGLHLASVTNVSCCASPERIPEEPMEVPEEVSDKSRITEIDEAKVLSSEEGAVTSSESKADSETPDPFSLDEKPLKSDILANVEQAQSSAPIHPDHQAEPTSGETSGSSSPISVMDRDSDISGARAKVRLLEEEADIQATHPRKRKMPRASPLAQANLTAQQVREKTQQSLAAIVDALKLEEIQPYQTERANPYYEYLHIRKKIEEKRKVLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKLCIPTITPPPSLPEPLKEMFKQQEMVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNMPQEVQGDDGKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSTSIFEIPEFYIPLVEVNDDFDLTPI

Target ORF information:

RefSeq Version	XM_017357915.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat domain 12 (ankrd12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357915.1

ORF Insert Sequence:

ATGGCCAAAC CGGGATCCGA CCGAGATGAA GCCATGGTGG AGAAGACAGC GGGGAAGAAG 
AGTAAAGAGA AAATCTCTCC GTTTGCTAAA ACTCCGAAGC TGGATCGGAG TGAACTCTTG 
GGGAAAGAGG GGAAGTCGAA ATCCTCCATG AAGCGGAAAC TCTCCTTCAC TGTCAGTCCA 
TCCCGAAACG AGGAGCGCGA CTCGGATGCA GATGACTCTG ACCCGGGGCA GTCGAGCGAT 
GCGTGGGGAG AGAGATTACT TCCTCCCTGC AGGATATACG CAGACAAAGA TGGACCGGAT 
AAGAAGAAGG TTAAGAAGGA GTCGGGGGGT AAGAAGTCGA CACCAGTGAA TATTTTGTTC 
GGGTACCCTT TATCAGAACG AAAGCAGATG GCACTTGTCA TGCAGATGAC GGCGAGAGAC 
AACAGTCCAG ATACCACACC GAACCACCCG TCTCAATCTC CGGCGGTGCA GAAGAAGATC 
GCCAGCAGCT CAACCTCACG ACAAAAAGAC AAAGTGAACA AACGCAACGA GAGAGGAGAG 
ACGCCTCTGC ATATGGCCGC CATCAGAGGA GACGTCAAAC AAGTGAAGGA GCTCATCGGC 
CTCGGAGCGG ATGTAAACGT CAAAGACTTT GCAGGTTGGA CGCCTCTCCA TGAAGCCTGC 
AACCTTGGTT ACTATGATGT AGCCAAGGTT TTGATTGGTG CAGGAGCGGA AGTGAACACG 
CAGGGATTGG ACGATGACAC GCCGCTTCAT GACGCCTCAA GCAGTGGACA CAAAGACATT 
GTGAAGCTGC TCTTGCGTCA TGGAGGCAAT GCTTTCCAAG CCAACAAGAG AGGGGAGAGA 
CCGGTTGATG TTGCTGACTC TCAGGAGCTG GAGCAGCTAC TCAAGGGAGA AATCCCACTG 
TCAGAACCAG AAGACAGCTC TTCAGAATCA GAGGACCCAT CTTCAGTGAA TCCATCCAGT 
GTAGACGAGA ACCTGGAGTT TTCTGATGCT GAAAAAGACT CTGACTGTAA ATCGACAACT 
GTCAAAGCCT CATCGTCCAT GTCAGGGCTG GATGAATATG AGTTTAAAGA TGAGGAAGAG 
GAAGAGGACC TCAGTAAGTC ACTTAATGAG CGACACCTCT TTAGGCGAGA GCTACGGCAA 
AAGGAGAAGG AGGAAAAGGA CAGAAACCAC TATGCTTCAA AACAAGGTAG CAAAAGCGAC 
CAGTCCATGC TGGCTTGCAA GTCTAAAAAA ACAAAGGCGT CCCGTGTCTA CTGCAGTTCA 
GACAGTTCAA GCGATGAAGC AGAGGTGCAG ACGGAAAGAA GGAGCTCACC TACTAGGTCA 
GTCAGTGTGG ATGGACATAA AACGGACAGT AGATCGAAAA AAGAAAGCTT GACTCTAACG 
CCAGTGGAGC AGAAAGAAAA AGGTAAACAG AAGAAAAAGA ACAAGAGCCA AAGCAAAAAC 
AAAGAAAACC AGGAAGTCAG AGAAGAAGGG AAAGAGAACA GCAAATCCCT TCTGTTTTCC 
TCTCCGACTG TGTCCGATAA TTCTGACAAG GGCGTCAGAG AAGAGGACTC TTTCAAAATG 
TCATTCAGTG CAAAGGATGA CACATCGGTT CACCTATTTC ACCTGTCTGT AGTCAAATCC 
CCAAAGCTGA ACCACAGCCA AACTGACAAG CAGGCAACCC CGCTGAAACA GGAAAACGCT 
AAGACATGTG TTTCAATCGC TGATGGATCT TGCCCTGGGG ACGGCATCAA ATATAACCAC 
TACACAGACT CTGACTATTG CACAGAAGAT TCTAGCAGTA AAAGCTGCAA ACATAAGGAA 
AAGAGCAAGC ACCATCACAA GGAGCCTAAC TTGGAAGGAG ATGACGGCAG TTCGAGTCCT 
TTCAAAGACA GCAGTTTAAG CAACAGTATA GACAGCTCTG AGGGTGCGTT TAGGAAGACT 
GACAAAGATG GGAAAGTTGT AAAAAAGCAC AAGCTAAAAC ACAAGGAGAA GGAAAAGGAG 
AAATATAGGA AAGAGTACGA GGCAGAGAGG AATCGCCACC GGCAGAAAGA GCCACGCAAG 
GACGGACATA GAAACCTCGA GTTTGATCGA GAGTTCTGGA AGGAGAACTT CTTCAAAAGT 
GATGAAAATG AGGAACCTGT GGGCAAAGGT GAGATACCTG CTGGTGGATC TCCAGTAAAA 
TCCTCAGATT CGTCACCTGT TAAAGAGGAA AAAGGGGCAT CAAAAGACAA ACATTCAACT 
AGTAGCAGTA ACAAGGACAG GAGGCCAAAA GAGGAAAGAG ATAAAACCAT TAAAAAGGAG 
AAAAAAGAGA TTCCTCTTAA AGAGGAGCGA GGAAAAGAAG ATGAAACAGA TGAGCGGACT 
GATGGTACTT TCTCTGTACG AATTCCTGAT GGCTCACAAC ACTTGTCAGA AGAAGTGGAT 
GAGAAACCAG CAACTTGTAA TTCGGCTGAC CAAGAGCAAC TGGAGTCTCC AGAGAAGAAC 
TCTCGAGAAA AAACTGATAA GCGACCACCA ACAAAGGATC GGGAATCTGA GAAGTCTGAT 
AAGAGACACT CGGATAAAGA TAAGAAGGTA AAATCTGAGC ATTTAGCTGA CAAGCCTGAG 
CTCCACAACT CTACAGATCG ATGGAAGGAA AAGGAGAAAT TAACAAATGT TTCATATTCG 
CCCAATGATA AGAGCCATAA AGAGGGTGAA AAACTCAAAC CAATGACTCC AGTGAAAAAG 
CATGAGGAGA ACAAGAAGAA TAAGGACAAG CTTGATAAAA AAGCGGAGAA AGAGCACGCT 
TCTGGTGACC ACAGAGACAA AGATAAAACA ACTTCTGAAA AGAAAGGAAA AGCTCCTGAG 
AAAACTGCTG ATCATGGAAA ATCTGACCGC AGTAAAGACA AAGAACGGGA CAAAGATTCT 
GACAAGAGGA AAAAAGATAA ATCAACCACT CCATCTTCCT CTTCCTACTC GAATCTAAAG 
TCGTTATTGG AAGAAAAGAA AGGCTATGTT TGTGAGAATA ATAAGGTTGT CTCATCTAAA 
TCAAAGGAAG AGGTAACCAA GCCTTCAGAG AAAGACCGAG ATCGAAGAGA GAGGGACCGG 
GAGTCTGAAA GACATCGAGA TCGGGAACGA GATCGGCACA AGGAAAAGGA CAAAGATCGA 
TCCCAGTTTA ACAGGGATGG CAAAATTAAC AAACCGAAAC CAAGTGAAGT GGACTCCAAA 
TCTAAGGTTA CACCTATTCT AAAGGATACT CGTCCCAAAG AAAAGAGGCT GGTGAATGAT 
GACCTCATGA AGACCAGCTT TGAACGCATG CTCAGTCTCA AAGATCAGGA AATCGAACAG 
TGGCACAAAA AGCACTTGGA GAAAATCAAG CAGAAGGAAC GGGAGCGGCT AAAACAGCGT 
CCTGGAATTG ACCCTGCGAA ACAACAAAAA AACAAGGAAA AAATTAAATC ATCCTCTACG 
TCCACTGAGC CATGTCTAAC CAAGGAACTA ACTCGGTCAA AGAGCTCAGA AGCGCCAGAT 
GGACAACGTG AAAAGATCTT AAAGGACGCC ACTAGTGTGA GAACTCTCTC GCTAGATGCA 
AAAACCTTGG GGAAGAACGG TCCTATAATT GAAAACAATC TAAGTCGATC TCCGAGACCA 
GAAAGTGAAA AATCTGGTCT TATGTCAAGA TCAATATCAA TGATCTCTGT GGCAAGTTCA 
GAAGACTCCT GCCAGGCAAC TATTTTGACA CCTAGACCAA CTGACCAATA TGATTCTGAC 
ATGAACGTGG AAGCCTTAGA TTCGCAGCCA CCTTTACTTC AGTCTTCCCT TGTTGCACAG 
TCCTCCAGGT CGCCTAGTGT TCATGATAAA GATATTAGCA GTCTTCCCGA ATCATCGGCT 
CTCTGCAATC GCACACCATT GGCAAGCAGG CATGCATCAC CATGCTTAAG GGCTATTCTG 
GATGAAGAAG CCAAGGTACC AACAACTGAA ACAAAACCCA CAGAAGAGTC TCCGGTAATC 
AGTATTGCAT CACAACCAAC CAGCGAGCAA TCTCAAGTTC ATCTAACTGA GATTAGTGTC 
CCCCCTGTTC AGGAGACTCG GAGCAGTCAA AGTCTTACAA ACTCTTTTCC AGAAAATGAA 
ATTCTGTCTA GTAATGAAGC CGAAGTCGTC AGTTCCACAT GTCAAGTACA ACCTTCGCAG 
GCTAGTGCTA AAGACAGTGT AACACAAGCA AACAGTGTGT TTCCGAGCCA TTTGGAAGAG 
CAGAACAAGT TTTGCACCAC TAGTAGTCAA ATAGAGCATG CAAGTCTAGG TCAGCTTGAT 
GTCCCTGGTG CAGAGGGAGT AAAAAAGGAG GCTTCACTTG CTGTTGCATC ATTACAGTCA 
TCTCATACAT CAACTTCATC ATCAGATTCC AATGCACATT TGCCTTCGAG TAGTCAGCAT 
TGTGAGATTA GCTCTGCTTC CAGTGGTGAA CAGACAATGA CAACAGACTC TAGTTTGAGA 
ACAGAGCAAA GAGACAAACC ATTGGTTGCT TCAGAGGACA AGATTGGGGA ACATGTCACT 
AAAACTGATT GCGCGAGCAC TCCAAGTCCA TCTACAGCAT CCTGTAGATC CAGCTCTGAG 
GAATCTACTA AACCACTGCT GAAGGCGACC AGTGTTCAGG AAGATACTGA AAAGGGCAAT 
GAACAAGTGC AAGAAGAAAG TATCTCAAGG TTCTCCAATG ACCCATTGGT TGCAGTTGAT 
GCTCAACCAG AGAAAGAAGA GCAAGGCCTA CATTTGGCTT CGGTGACAAA TGTTTCTTGC 
TGTGCCAGTC CAGAAAGAAT CCCAGAGGAG CCCATGGAGG TTCCAGAGGA AGTTTCGGAT 
AAGAGCCGAA TCACAGAAAT TGATGAGGCC AAAGTCTTGT CTTCAGAAGA AGGTGCAGTG 
ACTTCATCTG AAAGTAAGGC TGACTCAGAA ACGCCAGACC CGTTTTCTCT TGACGAGAAG 
CCTTTGAAAT CGGACATTCT GGCTAATGTA GAACAGGCTC AAAGCAGTGC TCCCATCCAC 
CCTGATCATC AGGCAGAGCC AACCAGTGGA GAGACCTCTG GAAGTTCCTC TCCGATATCA 
GTCATGGACA GAGATTCAGA TATATCTGGG GCCAGAGCCA AAGTCAGACT CCTTGAAGAA 
GAAGCTGACA TTCAAGCGAC CCATCCAAGA AAACGAAAAA TGCCTAGAGC TTCTCCGCTA 
GCCCAAGCAA ATCTTACAGC TCAGCAGGTC AGAGAAAAGA CACAGCAGTC CCTCGCTGCG 
ATTGTAGATG CACTGAAGCT TGAGGAGATT CAGCCTTACC AGACAGAGAG AGCCAACCCA 
TATTATGAGT ACTTGCACAT TCGCAAAAAG ATCGAAGAGA AGAGGAAAGT CCTTTGTAGT 
GTCATTCCTC AAGCGCCTCA GTACTACGAC GAGTATGTGA CATTCAATGG GTCCTATCTG 
CTGGATGGAA ACCCTCTCAG CAAACTCTGC ATCCCAACAA TAACGCCTCC ACCCTCTTTA 
CCTGAACCAC TGAAGGAGAT GTTCAAACAG CAAGAGATGG TGCGGATGAA GCTGAGACTG 
CAGCACAGCA TCGAAAGGGA AAAACTGATT GTATCCAATG AGCAGGAGGT TTTGAGAGTT 
CATTACCGCG CTGCGAGAAC ACTAGCCAAT CAGACGCTTC CGTTCAGTGC CTGCACAGTC 
CTATTGGATG CCGAAGTATA CAACATGCCC CAAGAAGTCC AGGGTGATGA TGGGAAGACT 
TCTGTTCGAG ATCGATTCAA TGCGAGGCAG TTCATGTCTT GGCTTCAGGA TGTAGACGAC 
AAGTTTGACA AGTTGAAGAC GTGTCTCCTG ATGCGGCAGC AGCATGAAGC GGCAGCGTTA 
AACGCAGTCC AGCGTCTGGA GTGGCAGCTA AAGCTACAGG AACTGGATCC CGCCACTTAC 
AAGTCCACCA GCATTTTCGA GATTCCCGAA TTCTACATCC CGCTCGTGGA GGTCAACGAC 
GACTTCGACC TGACGCCGAT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankrd12 ( XM_017357916.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213405.1 Danio rerio ankyrin repeat domain 12 (ankrd12), transcript variant X2, mRNA.

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CloneID	ODa56270
Clone ID Related Accession (Same CDS sequence)	XM_017357916.1 , XM_017357916.2
Accession Version	XM_017357916.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CGGGATCCGA CCGAGATGAA GCCATGGTGG AGAAGACAGC GGGGAAGAAG 
AGTAAAGAGA AAATCTCTCC GTTTGCTAAA ACTCCGAAGC TGGATCGGAG TGAACTCTTG 
GGGAAAGAGG GGAAGTCGAA ATCCTCCATG AAGCGGAAAC TCTCCTTCAC TGTCAGTCCA 
TCCCGAAACG AGGAGCGCGA CTCGGATGCA GACAAAGATG GACCGGATAA GAAGAAGGTT 
AAGAAGGAGT CGGGGGGTAA GAAGTCGACA CCAGTGAATA TTTTGTTCGG GTACCCTTTA 
TCAGAACGAA AGCAGATGGC ACTTGTCATG CAGATGACGG CGAGAGACAA CAGTCCAGAT 
ACCACACCGA ACCACCCGTC TCAATCTCCG GCGGTGCAGA AGAAGATCGC CAGCAGCTCA 
ACCTCACGAC AAAAAGACAA AGTGAACAAA CGCAACGAGA GAGGAGAGAC GCCTCTGCAT 
ATGGCCGCCA TCAGAGGAGA CGTCAAACAA GTGAAGGAGC TCATCGGCCT CGGAGCGGAT 
GTAAACGTCA AAGACTTTGC AGGTTGGACG CCTCTCCATG AAGCCTGCAA CCTTGGTTAC 
TATGATGTAG CCAAGGTTTT GATTGGTGCA GGAGCGGAAG TGAACACGCA GGGATTGGAC 
GATGACACGC CGCTTCATGA CGCCTCAAGC AGTGGACACA AAGACATTGT GAAGCTGCTC 
TTGCGTCATG GAGGCAATGC TTTCCAAGCC AACAAGAGAG GGGAGAGACC GGTTGATGTT 
GCTGACTCTC AGGAGCTGGA GCAGCTACTC AAGGGAGAAA TCCCACTGTC AGAACCAGAA 
GACAGCTCTT CAGAATCAGA GGACCCATCT TCAGTGAATC CATCCAGTGT AGACGAGAAC 
CTGGAGTTTT CTGATGCTGA AAAAGACTCT GACTGTAAAT CGACAACTGT CAAAGCCTCA 
TCGTCCATGT CAGGGCTGGA TGAATATGAG TTTAAAGATG AGGAAGAGGA AGAGGACCTC 
AGTAAGTCAC TTAATGAGCG ACACCTCTTT AGGCGAGAGC TACGGCAAAA GGAGAAGGAG 
GAAAAGGACA GAAACCACTA TGCTTCAAAA CAAGGTAGCA AAAGCGACCA GTCCATGCTG 
GCTTGCAAGT CTAAAAAAAC AAAGGCGTCC CGTGTCTACT GCAGTTCAGA CAGTTCAAGC 
GATGAAGCAG AGGTGCAGAC GGAAAGAAGG AGCTCACCTA CTAGGTCAGT CAGTGTGGAT 
GGACATAAAA CGGACAGTAG ATCGAAAAAA GAAAGCTTGA CTCTAACGCC AGTGGAGCAG 
AAAGAAAAAG GTAAACAGAA GAAAAAGAAC AAGAGCCAAA GCAAAAACAA AGAAAACCAG 
GAAGTCAGAG AAGAAGGGAA AGAGAACAGC AAATCCCTTC TGTTTTCCTC TCCGACTGTG 
TCCGATAATT CTGACAAGGG CGTCAGAGAA GAGGACTCTT TCAAAATGTC ATTCAGTGCA 
AAGGATGACA CATCGGTTCA CCTATTTCAC CTGTCTGTAG TCAAATCCCC AAAGCTGAAC 
CACAGCCAAA CTGACAAGCA GGCAACCCCG CTGAAACAGG AAAACGCTAA GACATGTGTT 
TCAATCGCTG ATGGATCTTG CCCTGGGGAC GGCATCAAAT ATAACCACTA CACAGACTCT 
GACTATTGCA CAGAAGATTC TAGCAGTAAA AGCTGCAAAC ATAAGGAAAA GAGCAAGCAC 
CATCACAAGG AGCCTAACTT GGAAGGAGAT GACGGCAGTT CGAGTCCTTT CAAAGACAGC 
AGTTTAAGCA ACAGTATAGA CAGCTCTGAG GGTGCGTTTA GGAAGACTGA CAAAGATGGG 
AAAGTTGTAA AAAAGCACAA GCTAAAACAC AAGGAGAAGG AAAAGGAGAA ATATAGGAAA 
GAGTACGAGG CAGAGAGGAA TCGCCACCGG CAGAAAGAGC CACGCAAGGA CGGACATAGA 
AACCTCGAGT TTGATCGAGA GTTCTGGAAG GAGAACTTCT TCAAAAGTGA TGAAAATGAG 
GAACCTGTGG GCAAAGGTGA GATACCTGCT GGTGGATCTC CAGTAAAATC CTCAGATTCG 
TCACCTGTTA AAGAGGAAAA AGGGGCATCA AAAGACAAAC ATTCAACTAG TAGCAGTAAC 
AAGGACAGGA GGCCAAAAGA GGAAAGAGAT AAAACCATTA AAAAGGAGAA AAAAGAGATT 
CCTCTTAAAG AGGAGCGAGG AAAAGAAGAT GAAACAGATG AGCGGACTGA TGGTACTTTC 
TCTGTACGAA TTCCTGATGG CTCACAACAC TTGTCAGAAG AAGTGGATGA GAAACCAGCA 
ACTTGTAATT CGGCTGACCA AGAGCAACTG GAGTCTCCAG AGAAGAACTC TCGAGAAAAA 
ACTGATAAGC GACCACCAAC AAAGGATCGG GAATCTGAGA AGTCTGATAA GAGACACTCG 
GATAAAGATA AGAAGGTAAA ATCTGAGCAT TTAGCTGACA AGCCTGAGCT CCACAACTCT 
ACAGATCGAT GGAAGGAAAA GGAGAAATTA ACAAATGTTT CATATTCGCC CAATGATAAG 
AGCCATAAAG AGGGTGAAAA ACTCAAACCA ATGACTCCAG TGAAAAAGCA TGAGGAGAAC 
AAGAAGAATA AGGACAAGCT TGATAAAAAA GCGGAGAAAG AGCACGCTTC TGGTGACCAC 
AGAGACAAAG ATAAAACAAC TTCTGAAAAG AAAGGAAAAG CTCCTGAGAA AACTGCTGAT 
CATGGAAAAT CTGACCGCAG TAAAGACAAA GAACGGGACA AAGATTCTGA CAAGAGGAAA 
AAAGATAAAT CAACCACTCC ATCTTCCTCT TCCTACTCGA ATCTAAAGTC GTTATTGGAA 
GAAAAGAAAG GCTATGTTTG TGAGAATAAT AAGGTTGTCT CATCTAAATC AAAGGAAGAG 
GTAACCAAGC CTTCAGAGAA AGACCGAGAT CGAAGAGAGA GGGACCGGGA GTCTGAAAGA 
CATCGAGATC GGGAACGAGA TCGGCACAAG GAAAAGGACA AAGATCGATC CCAGTTTAAC 
AGGGATGGCA AAATTAACAA ACCGAAACCA AGTGAAGTGG ACTCCAAATC TAAGGTTACA 
CCTATTCTAA AGGATACTCG TCCCAAAGAA AAGAGGCTGG TGAATGATGA CCTCATGAAG 
ACCAGCTTTG AACGCATGCT CAGTCTCAAA GATCAGGAAA TCGAACAGTG GCACAAAAAG 
CACTTGGAGA AAATCAAGCA GAAGGAACGG GAGCGGCTAA AACAGCGTCC TGGAATTGAC 
CCTGCGAAAC AACAAAAAAA CAAGGAAAAA ATTAAATCAT CCTCTACGTC CACTGAGCCA 
TGTCTAACCA AGGAACTAAC TCGGTCAAAG AGCTCAGAAG CGCCAGATGG ACAACGTGAA 
AAGATCTTAA AGGACGCCAC TAGTGTGAGA ACTCTCTCGC TAGATGCAAA AACCTTGGGG 
AAGAACGGTC CTATAATTGA AAACAATCTA AGTCGATCTC CGAGACCAGA AAGTGAAAAA 
TCTGGTCTTA TGTCAAGATC AATATCAATG ATCTCTGTGG CAAGTTCAGA AGACTCCTGC 
CAGGCAACTA TTTTGACACC TAGACCAACT GACCAATATG ATTCTGACAT GAACGTGGAA 
GCCTTAGATT CGCAGCCACC TTTACTTCAG TCTTCCCTTG TTGCACAGTC CTCCAGGTCG 
CCTAGTGTTC ATGATAAAGA TATTAGCAGT CTTCCCGAAT CATCGGCTCT CTGCAATCGC 
ACACCATTGG CAAGCAGGCA TGCATCACCA TGCTTAAGGG CTATTCTGGA TGAAGAAGCC 
AAGGTACCAA CAACTGAAAC AAAACCCACA GAAGAGTCTC CGGTAATCAG TATTGCATCA 
CAACCAACCA GCGAGCAATC TCAAGTTCAT CTAACTGAGA TTAGTGTCCC CCCTGTTCAG 
GAGACTCGGA GCAGTCAAAG TCTTACAAAC TCTTTTCCAG AAAATGAAAT TCTGTCTAGT 
AATGAAGCCG AAGTCGTCAG TTCCACATGT CAAGTACAAC CTTCGCAGGC TAGTGCTAAA 
GACAGTGTAA CACAAGCAAA CAGTGTGTTT CCGAGCCATT TGGAAGAGCA GAACAAGTTT 
TGCACCACTA GTAGTCAAAT AGAGCATGCA AGTCTAGGTC AGCTTGATGT CCCTGGTGCA 
GAGGGAGTAA AAAAGGAGGC TTCACTTGCT GTTGCATCAT TACAGTCATC TCATACATCA 
ACTTCATCAT CAGATTCCAA TGCACATTTG CCTTCGAGTA GTCAGCATTG TGAGATTAGC 
TCTGCTTCCA GTGGTGAACA GACAATGACA ACAGACTCTA GTTTGAGAAC AGAGCAAAGA 
GACAAACCAT TGGTTGCTTC AGAGGACAAG ATTGGGGAAC ATGTCACTAA AACTGATTGC 
GCGAGCACTC CAAGTCCATC TACAGCATCC TGTAGATCCA GCTCTGAGGA ATCTACTAAA 
CCACTGCTGA AGGCGACCAG TGTTCAGGAA GATACTGAAA AGGGCAATGA ACAAGTGCAA 
GAAGAAAGTA TCTCAAGGTT CTCCAATGAC CCATTGGTTG CAGTTGATGC TCAACCAGAG 
AAAGAAGAGC AAGGCCTACA TTTGGCTTCG GTGACAAATG TTTCTTGCTG TGCCAGTCCA 
GAAAGAATCC CAGAGGAGCC CATGGAGGTT CCAGAGGAAG TTTCGGATAA GAGCCGAATC 
ACAGAAATTG ATGAGGCCAA AGTCTTGTCT TCAGAAGAAG GTGCAGTGAC TTCATCTGAA 
AGTAAGGCTG ACTCAGAAAC GCCAGACCCG TTTTCTCTTG ACGAGAAGCC TTTGAAATCG 
GACATTCTGG CTAATGTAGA ACAGGCTCAA AGCAGTGCTC CCATCCACCC TGATCATCAG 
GCAGAGCCAA CCAGTGGAGA GACCTCTGGA AGTTCCTCTC CGATATCAGT CATGGACAGA 
GATTCAGATA TATCTGGGGC CAGAGCCAAA GTCAGACTCC TTGAAGAAGA AGCTGACATT 
CAAGCGACCC ATCCAAGAAA ACGAAAAATG CCTAGAGCTT CTCCGCTAGC CCAAGCAAAT 
CTTACAGCTC AGCAGGTCAG AGAAAAGACA CAGCAGTCCC TCGCTGCGAT TGTAGATGCA 
CTGAAGCTTG AGGAGATTCA GCCTTACCAG ACAGAGAGAG CCAACCCATA TTATGAGTAC 
TTGCACATTC GCAAAAAGAT CGAAGAGAAG AGGAAAGTCC TTTGTAGTGT CATTCCTCAA 
GCGCCTCAGT ACTACGACGA GTATGTGACA TTCAATGGGT CCTATCTGCT GGATGGAAAC 
CCTCTCAGCA AACTCTGCAT CCCAACAATA ACGCCTCCAC CCTCTTTACC TGAACCACTG 
AAGGAGATGT TCAAACAGCA AGAGATGGTG CGGATGAAGC TGAGACTGCA GCACAGCATC 
GAAAGGGAAA AACTGATTGT ATCCAATGAG CAGGAGGTTT TGAGAGTTCA TTACCGCGCT 
GCGAGAACAC TAGCCAATCA GACGCTTCCG TTCAGTGCCT GCACAGTCCT ATTGGATGCC 
GAAGTATACA ACATGCCCCA AGAAGTCCAG GGTGATGATG GGAAGACTTC TGTTCGAGAT 
CGATTCAATG CGAGGCAGTT CATGTCTTGG CTTCAGGATG TAGACGACAA GTTTGACAAG 
TTGAAGACGT GTCTCCTGAT GCGGCAGCAG CATGAAGCGG CAGCGTTAAA CGCAGTCCAG 
CGTCTGGAGT GGCAGCTAAA GCTACAGGAA CTGGATCCCG CCACTTACAA GTCCACCAGC 
ATTTTCGAGA TTCCCGAATT CTACATCCCG CTCGTGGAGG TCAACGACGA CTTCGACCTG 
ACGCCGATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213405.1
CDS	259..6150
Translation	MAKPGSDRDEAMVEKTAGKKSKEKISPFAKTPKLDRSELLGKEGKSKSSMKRKLSFTVSPSRNEERDSDADKDGPDKKKVKKESGGKKSTPVNILFGYPLSERKQMALVMQMTARDNSPDTTPNHPSQSPAVQKKIASSSTSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELIGLGADVNVKDFAGWTPLHEACNLGYYDVAKVLIGAGAEVNTQGLDDDTPLHDASSSGHKDIVKLLLRHGGNAFQANKRGERPVDVADSQELEQLLKGEIPLSEPEDSSSESEDPSSVNPSSVDENLEFSDAEKDSDCKSTTVKASSSMSGLDEYEFKDEEEEEDLSKSLNERHLFRRELRQKEKEEKDRNHYASKQGSKSDQSMLACKSKKTKASRVYCSSDSSSDEAEVQTERRSSPTRSVSVDGHKTDSRSKKESLTLTPVEQKEKGKQKKKNKSQSKNKENQEVREEGKENSKSLLFSSPTVSDNSDKGVREEDSFKMSFSAKDDTSVHLFHLSVVKSPKLNHSQTDKQATPLKQENAKTCVSIADGSCPGDGIKYNHYTDSDYCTEDSSSKSCKHKEKSKHHHKEPNLEGDDGSSSPFKDSSLSNSIDSSEGAFRKTDKDGKVVKKHKLKHKEKEKEKYRKEYEAERNRHRQKEPRKDGHRNLEFDREFWKENFFKSDENEEPVGKGEIPAGGSPVKSSDSSPVKEEKGASKDKHSTSSSNKDRRPKEERDKTIKKEKKEIPLKEERGKEDETDERTDGTFSVRIPDGSQHLSEEVDEKPATCNSADQEQLESPEKNSREKTDKRPPTKDRESEKSDKRHSDKDKKVKSEHLADKPELHNSTDRWKEKEKLTNVSYSPNDKSHKEGEKLKPMTPVKKHEENKKNKDKLDKKAEKEHASGDHRDKDKTTSEKKGKAPEKTADHGKSDRSKDKERDKDSDKRKKDKSTTPSSSSYSNLKSLLEEKKGYVCENNKVVSSKSKEEVTKPSEKDRDRRERDRESERHRDRERDRHKEKDKDRSQFNRDGKINKPKPSEVDSKSKVTPILKDTRPKEKRLVNDDLMKTSFERMLSLKDQEIEQWHKKHLEKIKQKERERLKQRPGIDPAKQQKNKEKIKSSSTSTEPCLTKELTRSKSSEAPDGQREKILKDATSVRTLSLDAKTLGKNGPIIENNLSRSPRPESEKSGLMSRSISMISVASSEDSCQATILTPRPTDQYDSDMNVEALDSQPPLLQSSLVAQSSRSPSVHDKDISSLPESSALCNRTPLASRHASPCLRAILDEEAKVPTTETKPTEESPVISIASQPTSEQSQVHLTEISVPPVQETRSSQSLTNSFPENEILSSNEAEVVSSTCQVQPSQASAKDSVTQANSVFPSHLEEQNKFCTTSSQIEHASLGQLDVPGAEGVKKEASLAVASLQSSHTSTSSSDSNAHLPSSSQHCEISSASSGEQTMTTDSSLRTEQRDKPLVASEDKIGEHVTKTDCASTPSPSTASCRSSSEESTKPLLKATSVQEDTEKGNEQVQEESISRFSNDPLVAVDAQPEKEEQGLHLASVTNVSCCASPERIPEEPMEVPEEVSDKSRITEIDEAKVLSSEEGAVTSSESKADSETPDPFSLDEKPLKSDILANVEQAQSSAPIHPDHQAEPTSGETSGSSSPISVMDRDSDISGARAKVRLLEEEADIQATHPRKRKMPRASPLAQANLTAQQVREKTQQSLAAIVDALKLEEIQPYQTERANPYYEYLHIRKKIEEKRKVLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKLCIPTITPPPSLPEPLKEMFKQQEMVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNMPQEVQGDDGKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSTSIFEIPEFYIPLVEVNDDFDLTPI

Target ORF information:

RefSeq Version	XM_017357916.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin repeat domain 12 (ankrd12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357916.1

ORF Insert Sequence:

ATGGCCAAAC CGGGATCCGA CCGAGATGAA GCCATGGTGG AGAAGACAGC GGGGAAGAAG 
AGTAAAGAGA AAATCTCTCC GTTTGCTAAA ACTCCGAAGC TGGATCGGAG TGAACTCTTG 
GGGAAAGAGG GGAAGTCGAA ATCCTCCATG AAGCGGAAAC TCTCCTTCAC TGTCAGTCCA 
TCCCGAAACG AGGAGCGCGA CTCGGATGCA GACAAAGATG GACCGGATAA GAAGAAGGTT 
AAGAAGGAGT CGGGGGGTAA GAAGTCGACA CCAGTGAATA TTTTGTTCGG GTACCCTTTA 
TCAGAACGAA AGCAGATGGC ACTTGTCATG CAGATGACGG CGAGAGACAA CAGTCCAGAT 
ACCACACCGA ACCACCCGTC TCAATCTCCG GCGGTGCAGA AGAAGATCGC CAGCAGCTCA 
ACCTCACGAC AAAAAGACAA AGTGAACAAA CGCAACGAGA GAGGAGAGAC GCCTCTGCAT 
ATGGCCGCCA TCAGAGGAGA CGTCAAACAA GTGAAGGAGC TCATCGGCCT CGGAGCGGAT 
GTAAACGTCA AAGACTTTGC AGGTTGGACG CCTCTCCATG AAGCCTGCAA CCTTGGTTAC 
TATGATGTAG CCAAGGTTTT GATTGGTGCA GGAGCGGAAG TGAACACGCA GGGATTGGAC 
GATGACACGC CGCTTCATGA CGCCTCAAGC AGTGGACACA AAGACATTGT GAAGCTGCTC 
TTGCGTCATG GAGGCAATGC TTTCCAAGCC AACAAGAGAG GGGAGAGACC GGTTGATGTT 
GCTGACTCTC AGGAGCTGGA GCAGCTACTC AAGGGAGAAA TCCCACTGTC AGAACCAGAA 
GACAGCTCTT CAGAATCAGA GGACCCATCT TCAGTGAATC CATCCAGTGT AGACGAGAAC 
CTGGAGTTTT CTGATGCTGA AAAAGACTCT GACTGTAAAT CGACAACTGT CAAAGCCTCA 
TCGTCCATGT CAGGGCTGGA TGAATATGAG TTTAAAGATG AGGAAGAGGA AGAGGACCTC 
AGTAAGTCAC TTAATGAGCG ACACCTCTTT AGGCGAGAGC TACGGCAAAA GGAGAAGGAG 
GAAAAGGACA GAAACCACTA TGCTTCAAAA CAAGGTAGCA AAAGCGACCA GTCCATGCTG 
GCTTGCAAGT CTAAAAAAAC AAAGGCGTCC CGTGTCTACT GCAGTTCAGA CAGTTCAAGC 
GATGAAGCAG AGGTGCAGAC GGAAAGAAGG AGCTCACCTA CTAGGTCAGT CAGTGTGGAT 
GGACATAAAA CGGACAGTAG ATCGAAAAAA GAAAGCTTGA CTCTAACGCC AGTGGAGCAG 
AAAGAAAAAG GTAAACAGAA GAAAAAGAAC AAGAGCCAAA GCAAAAACAA AGAAAACCAG 
GAAGTCAGAG AAGAAGGGAA AGAGAACAGC AAATCCCTTC TGTTTTCCTC TCCGACTGTG 
TCCGATAATT CTGACAAGGG CGTCAGAGAA GAGGACTCTT TCAAAATGTC ATTCAGTGCA 
AAGGATGACA CATCGGTTCA CCTATTTCAC CTGTCTGTAG TCAAATCCCC AAAGCTGAAC 
CACAGCCAAA CTGACAAGCA GGCAACCCCG CTGAAACAGG AAAACGCTAA GACATGTGTT 
TCAATCGCTG ATGGATCTTG CCCTGGGGAC GGCATCAAAT ATAACCACTA CACAGACTCT 
GACTATTGCA CAGAAGATTC TAGCAGTAAA AGCTGCAAAC ATAAGGAAAA GAGCAAGCAC 
CATCACAAGG AGCCTAACTT GGAAGGAGAT GACGGCAGTT CGAGTCCTTT CAAAGACAGC 
AGTTTAAGCA ACAGTATAGA CAGCTCTGAG GGTGCGTTTA GGAAGACTGA CAAAGATGGG 
AAAGTTGTAA AAAAGCACAA GCTAAAACAC AAGGAGAAGG AAAAGGAGAA ATATAGGAAA 
GAGTACGAGG CAGAGAGGAA TCGCCACCGG CAGAAAGAGC CACGCAAGGA CGGACATAGA 
AACCTCGAGT TTGATCGAGA GTTCTGGAAG GAGAACTTCT TCAAAAGTGA TGAAAATGAG 
GAACCTGTGG GCAAAGGTGA GATACCTGCT GGTGGATCTC CAGTAAAATC CTCAGATTCG 
TCACCTGTTA AAGAGGAAAA AGGGGCATCA AAAGACAAAC ATTCAACTAG TAGCAGTAAC 
AAGGACAGGA GGCCAAAAGA GGAAAGAGAT AAAACCATTA AAAAGGAGAA AAAAGAGATT 
CCTCTTAAAG AGGAGCGAGG AAAAGAAGAT GAAACAGATG AGCGGACTGA TGGTACTTTC 
TCTGTACGAA TTCCTGATGG CTCACAACAC TTGTCAGAAG AAGTGGATGA GAAACCAGCA 
ACTTGTAATT CGGCTGACCA AGAGCAACTG GAGTCTCCAG AGAAGAACTC TCGAGAAAAA 
ACTGATAAGC GACCACCAAC AAAGGATCGG GAATCTGAGA AGTCTGATAA GAGACACTCG 
GATAAAGATA AGAAGGTAAA ATCTGAGCAT TTAGCTGACA AGCCTGAGCT CCACAACTCT 
ACAGATCGAT GGAAGGAAAA GGAGAAATTA ACAAATGTTT CATATTCGCC CAATGATAAG 
AGCCATAAAG AGGGTGAAAA ACTCAAACCA ATGACTCCAG TGAAAAAGCA TGAGGAGAAC 
AAGAAGAATA AGGACAAGCT TGATAAAAAA GCGGAGAAAG AGCACGCTTC TGGTGACCAC 
AGAGACAAAG ATAAAACAAC TTCTGAAAAG AAAGGAAAAG CTCCTGAGAA AACTGCTGAT 
CATGGAAAAT CTGACCGCAG TAAAGACAAA GAACGGGACA AAGATTCTGA CAAGAGGAAA 
AAAGATAAAT CAACCACTCC ATCTTCCTCT TCCTACTCGA ATCTAAAGTC GTTATTGGAA 
GAAAAGAAAG GCTATGTTTG TGAGAATAAT AAGGTTGTCT CATCTAAATC AAAGGAAGAG 
GTAACCAAGC CTTCAGAGAA AGACCGAGAT CGAAGAGAGA GGGACCGGGA GTCTGAAAGA 
CATCGAGATC GGGAACGAGA TCGGCACAAG GAAAAGGACA AAGATCGATC CCAGTTTAAC 
AGGGATGGCA AAATTAACAA ACCGAAACCA AGTGAAGTGG ACTCCAAATC TAAGGTTACA 
CCTATTCTAA AGGATACTCG TCCCAAAGAA AAGAGGCTGG TGAATGATGA CCTCATGAAG 
ACCAGCTTTG AACGCATGCT CAGTCTCAAA GATCAGGAAA TCGAACAGTG GCACAAAAAG 
CACTTGGAGA AAATCAAGCA GAAGGAACGG GAGCGGCTAA AACAGCGTCC TGGAATTGAC 
CCTGCGAAAC AACAAAAAAA CAAGGAAAAA ATTAAATCAT CCTCTACGTC CACTGAGCCA 
TGTCTAACCA AGGAACTAAC TCGGTCAAAG AGCTCAGAAG CGCCAGATGG ACAACGTGAA 
AAGATCTTAA AGGACGCCAC TAGTGTGAGA ACTCTCTCGC TAGATGCAAA AACCTTGGGG 
AAGAACGGTC CTATAATTGA AAACAATCTA AGTCGATCTC CGAGACCAGA AAGTGAAAAA 
TCTGGTCTTA TGTCAAGATC AATATCAATG ATCTCTGTGG CAAGTTCAGA AGACTCCTGC 
CAGGCAACTA TTTTGACACC TAGACCAACT GACCAATATG ATTCTGACAT GAACGTGGAA 
GCCTTAGATT CGCAGCCACC TTTACTTCAG TCTTCCCTTG TTGCACAGTC CTCCAGGTCG 
CCTAGTGTTC ATGATAAAGA TATTAGCAGT CTTCCCGAAT CATCGGCTCT CTGCAATCGC 
ACACCATTGG CAAGCAGGCA TGCATCACCA TGCTTAAGGG CTATTCTGGA TGAAGAAGCC 
AAGGTACCAA CAACTGAAAC AAAACCCACA GAAGAGTCTC CGGTAATCAG TATTGCATCA 
CAACCAACCA GCGAGCAATC TCAAGTTCAT CTAACTGAGA TTAGTGTCCC CCCTGTTCAG 
GAGACTCGGA GCAGTCAAAG TCTTACAAAC TCTTTTCCAG AAAATGAAAT TCTGTCTAGT 
AATGAAGCCG AAGTCGTCAG TTCCACATGT CAAGTACAAC CTTCGCAGGC TAGTGCTAAA 
GACAGTGTAA CACAAGCAAA CAGTGTGTTT CCGAGCCATT TGGAAGAGCA GAACAAGTTT 
TGCACCACTA GTAGTCAAAT AGAGCATGCA AGTCTAGGTC AGCTTGATGT CCCTGGTGCA 
GAGGGAGTAA AAAAGGAGGC TTCACTTGCT GTTGCATCAT TACAGTCATC TCATACATCA 
ACTTCATCAT CAGATTCCAA TGCACATTTG CCTTCGAGTA GTCAGCATTG TGAGATTAGC 
TCTGCTTCCA GTGGTGAACA GACAATGACA ACAGACTCTA GTTTGAGAAC AGAGCAAAGA 
GACAAACCAT TGGTTGCTTC AGAGGACAAG ATTGGGGAAC ATGTCACTAA AACTGATTGC 
GCGAGCACTC CAAGTCCATC TACAGCATCC TGTAGATCCA GCTCTGAGGA ATCTACTAAA 
CCACTGCTGA AGGCGACCAG TGTTCAGGAA GATACTGAAA AGGGCAATGA ACAAGTGCAA 
GAAGAAAGTA TCTCAAGGTT CTCCAATGAC CCATTGGTTG CAGTTGATGC TCAACCAGAG 
AAAGAAGAGC AAGGCCTACA TTTGGCTTCG GTGACAAATG TTTCTTGCTG TGCCAGTCCA 
GAAAGAATCC CAGAGGAGCC CATGGAGGTT CCAGAGGAAG TTTCGGATAA GAGCCGAATC 
ACAGAAATTG ATGAGGCCAA AGTCTTGTCT TCAGAAGAAG GTGCAGTGAC TTCATCTGAA 
AGTAAGGCTG ACTCAGAAAC GCCAGACCCG TTTTCTCTTG ACGAGAAGCC TTTGAAATCG 
GACATTCTGG CTAATGTAGA ACAGGCTCAA AGCAGTGCTC CCATCCACCC TGATCATCAG 
GCAGAGCCAA CCAGTGGAGA GACCTCTGGA AGTTCCTCTC CGATATCAGT CATGGACAGA 
GATTCAGATA TATCTGGGGC CAGAGCCAAA GTCAGACTCC TTGAAGAAGA AGCTGACATT 
CAAGCGACCC ATCCAAGAAA ACGAAAAATG CCTAGAGCTT CTCCGCTAGC CCAAGCAAAT 
CTTACAGCTC AGCAGGTCAG AGAAAAGACA CAGCAGTCCC TCGCTGCGAT TGTAGATGCA 
CTGAAGCTTG AGGAGATTCA GCCTTACCAG ACAGAGAGAG CCAACCCATA TTATGAGTAC 
TTGCACATTC GCAAAAAGAT CGAAGAGAAG AGGAAAGTCC TTTGTAGTGT CATTCCTCAA 
GCGCCTCAGT ACTACGACGA GTATGTGACA TTCAATGGGT CCTATCTGCT GGATGGAAAC 
CCTCTCAGCA AACTCTGCAT CCCAACAATA ACGCCTCCAC CCTCTTTACC TGAACCACTG 
AAGGAGATGT TCAAACAGCA AGAGATGGTG CGGATGAAGC TGAGACTGCA GCACAGCATC 
GAAAGGGAAA AACTGATTGT ATCCAATGAG CAGGAGGTTT TGAGAGTTCA TTACCGCGCT 
GCGAGAACAC TAGCCAATCA GACGCTTCCG TTCAGTGCCT GCACAGTCCT ATTGGATGCC 
GAAGTATACA ACATGCCCCA AGAAGTCCAG GGTGATGATG GGAAGACTTC TGTTCGAGAT 
CGATTCAATG CGAGGCAGTT CATGTCTTGG CTTCAGGATG TAGACGACAA GTTTGACAAG 
TTGAAGACGT GTCTCCTGAT GCGGCAGCAG CATGAAGCGG CAGCGTTAAA CGCAGTCCAG 
CGTCTGGAGT GGCAGCTAAA GCTACAGGAA CTGGATCCCG CCACTTACAA GTCCACCAGC 
ATTTTCGAGA TTCCCGAATT CTACATCCCG CTCGTGGAGG TCAACGACGA CTTCGACCTG 
ACGCCGATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankrd12 ( XM_017357916.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213405.1 Danio rerio ankyrin repeat domain 12 (ankrd12), transcript variant X2, mRNA.

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CloneID	ODa56270
Clone ID Related Accession (Same CDS sequence)	XM_017357916.1 , XM_017357916.2
Accession Version	XM_017357916.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CGGGATCCGA CCGAGATGAA GCCATGGTGG AGAAGACAGC GGGGAAGAAG 
AGTAAAGAGA AAATCTCTCC GTTTGCTAAA ACTCCGAAGC TGGATCGGAG TGAACTCTTG 
GGGAAAGAGG GGAAGTCGAA ATCCTCCATG AAGCGGAAAC TCTCCTTCAC TGTCAGTCCA 
TCCCGAAACG AGGAGCGCGA CTCGGATGCA GACAAAGATG GACCGGATAA GAAGAAGGTT 
AAGAAGGAGT CGGGGGGTAA GAAGTCGACA CCAGTGAATA TTTTGTTCGG GTACCCTTTA 
TCAGAACGAA AGCAGATGGC ACTTGTCATG CAGATGACGG CGAGAGACAA CAGTCCAGAT 
ACCACACCGA ACCACCCGTC TCAATCTCCG GCGGTGCAGA AGAAGATCGC CAGCAGCTCA 
ACCTCACGAC AAAAAGACAA AGTGAACAAA CGCAACGAGA GAGGAGAGAC GCCTCTGCAT 
ATGGCCGCCA TCAGAGGAGA CGTCAAACAA GTGAAGGAGC TCATCGGCCT CGGAGCGGAT 
GTAAACGTCA AAGACTTTGC AGGTTGGACG CCTCTCCATG AAGCCTGCAA CCTTGGTTAC 
TATGATGTAG CCAAGGTTTT GATTGGTGCA GGAGCGGAAG TGAACACGCA GGGATTGGAC 
GATGACACGC CGCTTCATGA CGCCTCAAGC AGTGGACACA AAGACATTGT GAAGCTGCTC 
TTGCGTCATG GAGGCAATGC TTTCCAAGCC AACAAGAGAG GGGAGAGACC GGTTGATGTT 
GCTGACTCTC AGGAGCTGGA GCAGCTACTC AAGGGAGAAA TCCCACTGTC AGAACCAGAA 
GACAGCTCTT CAGAATCAGA GGACCCATCT TCAGTGAATC CATCCAGTGT AGACGAGAAC 
CTGGAGTTTT CTGATGCTGA AAAAGACTCT GACTGTAAAT CGACAACTGT CAAAGCCTCA 
TCGTCCATGT CAGGGCTGGA TGAATATGAG TTTAAAGATG AGGAAGAGGA AGAGGACCTC 
AGTAAGTCAC TTAATGAGCG ACACCTCTTT AGGCGAGAGC TACGGCAAAA GGAGAAGGAG 
GAAAAGGACA GAAACCACTA TGCTTCAAAA CAAGGTAGCA AAAGCGACCA GTCCATGCTG 
GCTTGCAAGT CTAAAAAAAC AAAGGCGTCC CGTGTCTACT GCAGTTCAGA CAGTTCAAGC 
GATGAAGCAG AGGTGCAGAC GGAAAGAAGG AGCTCACCTA CTAGGTCAGT CAGTGTGGAT 
GGACATAAAA CGGACAGTAG ATCGAAAAAA GAAAGCTTGA CTCTAACGCC AGTGGAGCAG 
AAAGAAAAAG GTAAACAGAA GAAAAAGAAC AAGAGCCAAA GCAAAAACAA AGAAAACCAG 
GAAGTCAGAG AAGAAGGGAA AGAGAACAGC AAATCCCTTC TGTTTTCCTC TCCGACTGTG 
TCCGATAATT CTGACAAGGG CGTCAGAGAA GAGGACTCTT TCAAAATGTC ATTCAGTGCA 
AAGGATGACA CATCGGTTCA CCTATTTCAC CTGTCTGTAG TCAAATCCCC AAAGCTGAAC 
CACAGCCAAA CTGACAAGCA GGCAACCCCG CTGAAACAGG AAAACGCTAA GACATGTGTT 
TCAATCGCTG ATGGATCTTG CCCTGGGGAC GGCATCAAAT ATAACCACTA CACAGACTCT 
GACTATTGCA CAGAAGATTC TAGCAGTAAA AGCTGCAAAC ATAAGGAAAA GAGCAAGCAC 
CATCACAAGG AGCCTAACTT GGAAGGAGAT GACGGCAGTT CGAGTCCTTT CAAAGACAGC 
AGTTTAAGCA ACAGTATAGA CAGCTCTGAG GGTGCGTTTA GGAAGACTGA CAAAGATGGG 
AAAGTTGTAA AAAAGCACAA GCTAAAACAC AAGGAGAAGG AAAAGGAGAA ATATAGGAAA 
GAGTACGAGG CAGAGAGGAA TCGCCACCGG CAGAAAGAGC CACGCAAGGA CGGACATAGA 
AACCTCGAGT TTGATCGAGA GTTCTGGAAG GAGAACTTCT TCAAAAGTGA TGAAAATGAG 
GAACCTGTGG GCAAAGGTGA GATACCTGCT GGTGGATCTC CAGTAAAATC CTCAGATTCG 
TCACCTGTTA AAGAGGAAAA AGGGGCATCA AAAGACAAAC ATTCAACTAG TAGCAGTAAC 
AAGGACAGGA GGCCAAAAGA GGAAAGAGAT AAAACCATTA AAAAGGAGAA AAAAGAGATT 
CCTCTTAAAG AGGAGCGAGG AAAAGAAGAT GAAACAGATG AGCGGACTGA TGGTACTTTC 
TCTGTACGAA TTCCTGATGG CTCACAACAC TTGTCAGAAG AAGTGGATGA GAAACCAGCA 
ACTTGTAATT CGGCTGACCA AGAGCAACTG GAGTCTCCAG AGAAGAACTC TCGAGAAAAA 
ACTGATAAGC GACCACCAAC AAAGGATCGG GAATCTGAGA AGTCTGATAA GAGACACTCG 
GATAAAGATA AGAAGGTAAA ATCTGAGCAT TTAGCTGACA AGCCTGAGCT CCACAACTCT 
ACAGATCGAT GGAAGGAAAA GGAGAAATTA ACAAATGTTT CATATTCGCC CAATGATAAG 
AGCCATAAAG AGGGTGAAAA ACTCAAACCA ATGACTCCAG TGAAAAAGCA TGAGGAGAAC 
AAGAAGAATA AGGACAAGCT TGATAAAAAA GCGGAGAAAG AGCACGCTTC TGGTGACCAC 
AGAGACAAAG ATAAAACAAC TTCTGAAAAG AAAGGAAAAG CTCCTGAGAA AACTGCTGAT 
CATGGAAAAT CTGACCGCAG TAAAGACAAA GAACGGGACA AAGATTCTGA CAAGAGGAAA 
AAAGATAAAT CAACCACTCC ATCTTCCTCT TCCTACTCGA ATCTAAAGTC GTTATTGGAA 
GAAAAGAAAG GCTATGTTTG TGAGAATAAT AAGGTTGTCT CATCTAAATC AAAGGAAGAG 
GTAACCAAGC CTTCAGAGAA AGACCGAGAT CGAAGAGAGA GGGACCGGGA GTCTGAAAGA 
CATCGAGATC GGGAACGAGA TCGGCACAAG GAAAAGGACA AAGATCGATC CCAGTTTAAC 
AGGGATGGCA AAATTAACAA ACCGAAACCA AGTGAAGTGG ACTCCAAATC TAAGGTTACA 
CCTATTCTAA AGGATACTCG TCCCAAAGAA AAGAGGCTGG TGAATGATGA CCTCATGAAG 
ACCAGCTTTG AACGCATGCT CAGTCTCAAA GATCAGGAAA TCGAACAGTG GCACAAAAAG 
CACTTGGAGA AAATCAAGCA GAAGGAACGG GAGCGGCTAA AACAGCGTCC TGGAATTGAC 
CCTGCGAAAC AACAAAAAAA CAAGGAAAAA ATTAAATCAT CCTCTACGTC CACTGAGCCA 
TGTCTAACCA AGGAACTAAC TCGGTCAAAG AGCTCAGAAG CGCCAGATGG ACAACGTGAA 
AAGATCTTAA AGGACGCCAC TAGTGTGAGA ACTCTCTCGC TAGATGCAAA AACCTTGGGG 
AAGAACGGTC CTATAATTGA AAACAATCTA AGTCGATCTC CGAGACCAGA AAGTGAAAAA 
TCTGGTCTTA TGTCAAGATC AATATCAATG ATCTCTGTGG CAAGTTCAGA AGACTCCTGC 
CAGGCAACTA TTTTGACACC TAGACCAACT GACCAATATG ATTCTGACAT GAACGTGGAA 
GCCTTAGATT CGCAGCCACC TTTACTTCAG TCTTCCCTTG TTGCACAGTC CTCCAGGTCG 
CCTAGTGTTC ATGATAAAGA TATTAGCAGT CTTCCCGAAT CATCGGCTCT CTGCAATCGC 
ACACCATTGG CAAGCAGGCA TGCATCACCA TGCTTAAGGG CTATTCTGGA TGAAGAAGCC 
AAGGTACCAA CAACTGAAAC AAAACCCACA GAAGAGTCTC CGGTAATCAG TATTGCATCA 
CAACCAACCA GCGAGCAATC TCAAGTTCAT CTAACTGAGA TTAGTGTCCC CCCTGTTCAG 
GAGACTCGGA GCAGTCAAAG TCTTACAAAC TCTTTTCCAG AAAATGAAAT TCTGTCTAGT 
AATGAAGCCG AAGTCGTCAG TTCCACATGT CAAGTACAAC CTTCGCAGGC TAGTGCTAAA 
GACAGTGTAA CACAAGCAAA CAGTGTGTTT CCGAGCCATT TGGAAGAGCA GAACAAGTTT 
TGCACCACTA GTAGTCAAAT AGAGCATGCA AGTCTAGGTC AGCTTGATGT CCCTGGTGCA 
GAGGGAGTAA AAAAGGAGGC TTCACTTGCT GTTGCATCAT TACAGTCATC TCATACATCA 
ACTTCATCAT CAGATTCCAA TGCACATTTG CCTTCGAGTA GTCAGCATTG TGAGATTAGC 
TCTGCTTCCA GTGGTGAACA GACAATGACA ACAGACTCTA GTTTGAGAAC AGAGCAAAGA 
GACAAACCAT TGGTTGCTTC AGAGGACAAG ATTGGGGAAC ATGTCACTAA AACTGATTGC 
GCGAGCACTC CAAGTCCATC TACAGCATCC TGTAGATCCA GCTCTGAGGA ATCTACTAAA 
CCACTGCTGA AGGCGACCAG TGTTCAGGAA GATACTGAAA AGGGCAATGA ACAAGTGCAA 
GAAGAAAGTA TCTCAAGGTT CTCCAATGAC CCATTGGTTG CAGTTGATGC TCAACCAGAG 
AAAGAAGAGC AAGGCCTACA TTTGGCTTCG GTGACAAATG TTTCTTGCTG TGCCAGTCCA 
GAAAGAATCC CAGAGGAGCC CATGGAGGTT CCAGAGGAAG TTTCGGATAA GAGCCGAATC 
ACAGAAATTG ATGAGGCCAA AGTCTTGTCT TCAGAAGAAG GTGCAGTGAC TTCATCTGAA 
AGTAAGGCTG ACTCAGAAAC GCCAGACCCG TTTTCTCTTG ACGAGAAGCC TTTGAAATCG 
GACATTCTGG CTAATGTAGA ACAGGCTCAA AGCAGTGCTC CCATCCACCC TGATCATCAG 
GCAGAGCCAA CCAGTGGAGA GACCTCTGGA AGTTCCTCTC CGATATCAGT CATGGACAGA 
GATTCAGATA TATCTGGGGC CAGAGCCAAA GTCAGACTCC TTGAAGAAGA AGCTGACATT 
CAAGCGACCC ATCCAAGAAA ACGAAAAATG CCTAGAGCTT CTCCGCTAGC CCAAGCAAAT 
CTTACAGCTC AGCAGGTCAG AGAAAAGACA CAGCAGTCCC TCGCTGCGAT TGTAGATGCA 
CTGAAGCTTG AGGAGATTCA GCCTTACCAG ACAGAGAGAG CCAACCCATA TTATGAGTAC 
TTGCACATTC GCAAAAAGAT CGAAGAGAAG AGGAAAGTCC TTTGTAGTGT CATTCCTCAA 
GCGCCTCAGT ACTACGACGA GTATGTGACA TTCAATGGGT CCTATCTGCT GGATGGAAAC 
CCTCTCAGCA AACTCTGCAT CCCAACAATA ACGCCTCCAC CCTCTTTACC TGAACCACTG 
AAGGAGATGT TCAAACAGCA AGAGATGGTG CGGATGAAGC TGAGACTGCA GCACAGCATC 
GAAAGGGAAA AACTGATTGT ATCCAATGAG CAGGAGGTTT TGAGAGTTCA TTACCGCGCT 
GCGAGAACAC TAGCCAATCA GACGCTTCCG TTCAGTGCCT GCACAGTCCT ATTGGATGCC 
GAAGTATACA ACATGCCCCA AGAAGTCCAG GGTGATGATG GGAAGACTTC TGTTCGAGAT 
CGATTCAATG CGAGGCAGTT CATGTCTTGG CTTCAGGATG TAGACGACAA GTTTGACAAG 
TTGAAGACGT GTCTCCTGAT GCGGCAGCAG CATGAAGCGG CAGCGTTAAA CGCAGTCCAG 
CGTCTGGAGT GGCAGCTAAA GCTACAGGAA CTGGATCCCG CCACTTACAA GTCCACCAGC 
ATTTTCGAGA TTCCCGAATT CTACATCCCG CTCGTGGAGG TCAACGACGA CTTCGACCTG 
ACGCCGATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213405.1
CDS	259..6150
Translation	MAKPGSDRDEAMVEKTAGKKSKEKISPFAKTPKLDRSELLGKEGKSKSSMKRKLSFTVSPSRNEERDSDADKDGPDKKKVKKESGGKKSTPVNILFGYPLSERKQMALVMQMTARDNSPDTTPNHPSQSPAVQKKIASSSTSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELIGLGADVNVKDFAGWTPLHEACNLGYYDVAKVLIGAGAEVNTQGLDDDTPLHDASSSGHKDIVKLLLRHGGNAFQANKRGERPVDVADSQELEQLLKGEIPLSEPEDSSSESEDPSSVNPSSVDENLEFSDAEKDSDCKSTTVKASSSMSGLDEYEFKDEEEEEDLSKSLNERHLFRRELRQKEKEEKDRNHYASKQGSKSDQSMLACKSKKTKASRVYCSSDSSSDEAEVQTERRSSPTRSVSVDGHKTDSRSKKESLTLTPVEQKEKGKQKKKNKSQSKNKENQEVREEGKENSKSLLFSSPTVSDNSDKGVREEDSFKMSFSAKDDTSVHLFHLSVVKSPKLNHSQTDKQATPLKQENAKTCVSIADGSCPGDGIKYNHYTDSDYCTEDSSSKSCKHKEKSKHHHKEPNLEGDDGSSSPFKDSSLSNSIDSSEGAFRKTDKDGKVVKKHKLKHKEKEKEKYRKEYEAERNRHRQKEPRKDGHRNLEFDREFWKENFFKSDENEEPVGKGEIPAGGSPVKSSDSSPVKEEKGASKDKHSTSSSNKDRRPKEERDKTIKKEKKEIPLKEERGKEDETDERTDGTFSVRIPDGSQHLSEEVDEKPATCNSADQEQLESPEKNSREKTDKRPPTKDRESEKSDKRHSDKDKKVKSEHLADKPELHNSTDRWKEKEKLTNVSYSPNDKSHKEGEKLKPMTPVKKHEENKKNKDKLDKKAEKEHASGDHRDKDKTTSEKKGKAPEKTADHGKSDRSKDKERDKDSDKRKKDKSTTPSSSSYSNLKSLLEEKKGYVCENNKVVSSKSKEEVTKPSEKDRDRRERDRESERHRDRERDRHKEKDKDRSQFNRDGKINKPKPSEVDSKSKVTPILKDTRPKEKRLVNDDLMKTSFERMLSLKDQEIEQWHKKHLEKIKQKERERLKQRPGIDPAKQQKNKEKIKSSSTSTEPCLTKELTRSKSSEAPDGQREKILKDATSVRTLSLDAKTLGKNGPIIENNLSRSPRPESEKSGLMSRSISMISVASSEDSCQATILTPRPTDQYDSDMNVEALDSQPPLLQSSLVAQSSRSPSVHDKDISSLPESSALCNRTPLASRHASPCLRAILDEEAKVPTTETKPTEESPVISIASQPTSEQSQVHLTEISVPPVQETRSSQSLTNSFPENEILSSNEAEVVSSTCQVQPSQASAKDSVTQANSVFPSHLEEQNKFCTTSSQIEHASLGQLDVPGAEGVKKEASLAVASLQSSHTSTSSSDSNAHLPSSSQHCEISSASSGEQTMTTDSSLRTEQRDKPLVASEDKIGEHVTKTDCASTPSPSTASCRSSSEESTKPLLKATSVQEDTEKGNEQVQEESISRFSNDPLVAVDAQPEKEEQGLHLASVTNVSCCASPERIPEEPMEVPEEVSDKSRITEIDEAKVLSSEEGAVTSSESKADSETPDPFSLDEKPLKSDILANVEQAQSSAPIHPDHQAEPTSGETSGSSSPISVMDRDSDISGARAKVRLLEEEADIQATHPRKRKMPRASPLAQANLTAQQVREKTQQSLAAIVDALKLEEIQPYQTERANPYYEYLHIRKKIEEKRKVLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKLCIPTITPPPSLPEPLKEMFKQQEMVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYNMPQEVQGDDGKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSTSIFEIPEFYIPLVEVNDDFDLTPI

Target ORF information:

RefSeq Version	XM_017357916.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat domain 12 (ankrd12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357916.2

ORF Insert Sequence:

ATGGCCAAAC CGGGATCCGA CCGAGATGAA GCCATGGTGG AGAAGACAGC GGGGAAGAAG 
AGTAAAGAGA AAATCTCTCC GTTTGCTAAA ACTCCGAAGC TGGATCGGAG TGAACTCTTG 
GGGAAAGAGG GGAAGTCGAA ATCCTCCATG AAGCGGAAAC TCTCCTTCAC TGTCAGTCCA 
TCCCGAAACG AGGAGCGCGA CTCGGATGCA GACAAAGATG GACCGGATAA GAAGAAGGTT 
AAGAAGGAGT CGGGGGGTAA GAAGTCGACA CCAGTGAATA TTTTGTTCGG GTACCCTTTA 
TCAGAACGAA AGCAGATGGC ACTTGTCATG CAGATGACGG CGAGAGACAA CAGTCCAGAT 
ACCACACCGA ACCACCCGTC TCAATCTCCG GCGGTGCAGA AGAAGATCGC CAGCAGCTCA 
ACCTCACGAC AAAAAGACAA AGTGAACAAA CGCAACGAGA GAGGAGAGAC GCCTCTGCAT 
ATGGCCGCCA TCAGAGGAGA CGTCAAACAA GTGAAGGAGC TCATCGGCCT CGGAGCGGAT 
GTAAACGTCA AAGACTTTGC AGGTTGGACG CCTCTCCATG AAGCCTGCAA CCTTGGTTAC 
TATGATGTAG CCAAGGTTTT GATTGGTGCA GGAGCGGAAG TGAACACGCA GGGATTGGAC 
GATGACACGC CGCTTCATGA CGCCTCAAGC AGTGGACACA AAGACATTGT GAAGCTGCTC 
TTGCGTCATG GAGGCAATGC TTTCCAAGCC AACAAGAGAG GGGAGAGACC GGTTGATGTT 
GCTGACTCTC AGGAGCTGGA GCAGCTACTC AAGGGAGAAA TCCCACTGTC AGAACCAGAA 
GACAGCTCTT CAGAATCAGA GGACCCATCT TCAGTGAATC CATCCAGTGT AGACGAGAAC 
CTGGAGTTTT CTGATGCTGA AAAAGACTCT GACTGTAAAT CGACAACTGT CAAAGCCTCA 
TCGTCCATGT CAGGGCTGGA TGAATATGAG TTTAAAGATG AGGAAGAGGA AGAGGACCTC 
AGTAAGTCAC TTAATGAGCG ACACCTCTTT AGGCGAGAGC TACGGCAAAA GGAGAAGGAG 
GAAAAGGACA GAAACCACTA TGCTTCAAAA CAAGGTAGCA AAAGCGACCA GTCCATGCTG 
GCTTGCAAGT CTAAAAAAAC AAAGGCGTCC CGTGTCTACT GCAGTTCAGA CAGTTCAAGC 
GATGAAGCAG AGGTGCAGAC GGAAAGAAGG AGCTCACCTA CTAGGTCAGT CAGTGTGGAT 
GGACATAAAA CGGACAGTAG ATCGAAAAAA GAAAGCTTGA CTCTAACGCC AGTGGAGCAG 
AAAGAAAAAG GTAAACAGAA GAAAAAGAAC AAGAGCCAAA GCAAAAACAA AGAAAACCAG 
GAAGTCAGAG AAGAAGGGAA AGAGAACAGC AAATCCCTTC TGTTTTCCTC TCCGACTGTG 
TCCGATAATT CTGACAAGGG CGTCAGAGAA GAGGACTCTT TCAAAATGTC ATTCAGTGCA 
AAGGATGACA CATCGGTTCA CCTATTTCAC CTGTCTGTAG TCAAATCCCC AAAGCTGAAC 
CACAGCCAAA CTGACAAGCA GGCAACCCCG CTGAAACAGG AAAACGCTAA GACATGTGTT 
TCAATCGCTG ATGGATCTTG CCCTGGGGAC GGCATCAAAT ATAACCACTA CACAGACTCT 
GACTATTGCA CAGAAGATTC TAGCAGTAAA AGCTGCAAAC ATAAGGAAAA GAGCAAGCAC 
CATCACAAGG AGCCTAACTT GGAAGGAGAT GACGGCAGTT CGAGTCCTTT CAAAGACAGC 
AGTTTAAGCA ACAGTATAGA CAGCTCTGAG GGTGCGTTTA GGAAGACTGA CAAAGATGGG 
AAAGTTGTAA AAAAGCACAA GCTAAAACAC AAGGAGAAGG AAAAGGAGAA ATATAGGAAA 
GAGTACGAGG CAGAGAGGAA TCGCCACCGG CAGAAAGAGC CACGCAAGGA CGGACATAGA 
AACCTCGAGT TTGATCGAGA GTTCTGGAAG GAGAACTTCT TCAAAAGTGA TGAAAATGAG 
GAACCTGTGG GCAAAGGTGA GATACCTGCT GGTGGATCTC CAGTAAAATC CTCAGATTCG 
TCACCTGTTA AAGAGGAAAA AGGGGCATCA AAAGACAAAC ATTCAACTAG TAGCAGTAAC 
AAGGACAGGA GGCCAAAAGA GGAAAGAGAT AAAACCATTA AAAAGGAGAA AAAAGAGATT 
CCTCTTAAAG AGGAGCGAGG AAAAGAAGAT GAAACAGATG AGCGGACTGA TGGTACTTTC 
TCTGTACGAA TTCCTGATGG CTCACAACAC TTGTCAGAAG AAGTGGATGA GAAACCAGCA 
ACTTGTAATT CGGCTGACCA AGAGCAACTG GAGTCTCCAG AGAAGAACTC TCGAGAAAAA 
ACTGATAAGC GACCACCAAC AAAGGATCGG GAATCTGAGA AGTCTGATAA GAGACACTCG 
GATAAAGATA AGAAGGTAAA ATCTGAGCAT TTAGCTGACA AGCCTGAGCT CCACAACTCT 
ACAGATCGAT GGAAGGAAAA GGAGAAATTA ACAAATGTTT CATATTCGCC CAATGATAAG 
AGCCATAAAG AGGGTGAAAA ACTCAAACCA ATGACTCCAG TGAAAAAGCA TGAGGAGAAC 
AAGAAGAATA AGGACAAGCT TGATAAAAAA GCGGAGAAAG AGCACGCTTC TGGTGACCAC 
AGAGACAAAG ATAAAACAAC TTCTGAAAAG AAAGGAAAAG CTCCTGAGAA AACTGCTGAT 
CATGGAAAAT CTGACCGCAG TAAAGACAAA GAACGGGACA AAGATTCTGA CAAGAGGAAA 
AAAGATAAAT CAACCACTCC ATCTTCCTCT TCCTACTCGA ATCTAAAGTC GTTATTGGAA 
GAAAAGAAAG GCTATGTTTG TGAGAATAAT AAGGTTGTCT CATCTAAATC AAAGGAAGAG 
GTAACCAAGC CTTCAGAGAA AGACCGAGAT CGAAGAGAGA GGGACCGGGA GTCTGAAAGA 
CATCGAGATC GGGAACGAGA TCGGCACAAG GAAAAGGACA AAGATCGATC CCAGTTTAAC 
AGGGATGGCA AAATTAACAA ACCGAAACCA AGTGAAGTGG ACTCCAAATC TAAGGTTACA 
CCTATTCTAA AGGATACTCG TCCCAAAGAA AAGAGGCTGG TGAATGATGA CCTCATGAAG 
ACCAGCTTTG AACGCATGCT CAGTCTCAAA GATCAGGAAA TCGAACAGTG GCACAAAAAG 
CACTTGGAGA AAATCAAGCA GAAGGAACGG GAGCGGCTAA AACAGCGTCC TGGAATTGAC 
CCTGCGAAAC AACAAAAAAA CAAGGAAAAA ATTAAATCAT CCTCTACGTC CACTGAGCCA 
TGTCTAACCA AGGAACTAAC TCGGTCAAAG AGCTCAGAAG CGCCAGATGG ACAACGTGAA 
AAGATCTTAA AGGACGCCAC TAGTGTGAGA ACTCTCTCGC TAGATGCAAA AACCTTGGGG 
AAGAACGGTC CTATAATTGA AAACAATCTA AGTCGATCTC CGAGACCAGA AAGTGAAAAA 
TCTGGTCTTA TGTCAAGATC AATATCAATG ATCTCTGTGG CAAGTTCAGA AGACTCCTGC 
CAGGCAACTA TTTTGACACC TAGACCAACT GACCAATATG ATTCTGACAT GAACGTGGAA 
GCCTTAGATT CGCAGCCACC TTTACTTCAG TCTTCCCTTG TTGCACAGTC CTCCAGGTCG 
CCTAGTGTTC ATGATAAAGA TATTAGCAGT CTTCCCGAAT CATCGGCTCT CTGCAATCGC 
ACACCATTGG CAAGCAGGCA TGCATCACCA TGCTTAAGGG CTATTCTGGA TGAAGAAGCC 
AAGGTACCAA CAACTGAAAC AAAACCCACA GAAGAGTCTC CGGTAATCAG TATTGCATCA 
CAACCAACCA GCGAGCAATC TCAAGTTCAT CTAACTGAGA TTAGTGTCCC CCCTGTTCAG 
GAGACTCGGA GCAGTCAAAG TCTTACAAAC TCTTTTCCAG AAAATGAAAT TCTGTCTAGT 
AATGAAGCCG AAGTCGTCAG TTCCACATGT CAAGTACAAC CTTCGCAGGC TAGTGCTAAA 
GACAGTGTAA CACAAGCAAA CAGTGTGTTT CCGAGCCATT TGGAAGAGCA GAACAAGTTT 
TGCACCACTA GTAGTCAAAT AGAGCATGCA AGTCTAGGTC AGCTTGATGT CCCTGGTGCA 
GAGGGAGTAA AAAAGGAGGC TTCACTTGCT GTTGCATCAT TACAGTCATC TCATACATCA 
ACTTCATCAT CAGATTCCAA TGCACATTTG CCTTCGAGTA GTCAGCATTG TGAGATTAGC 
TCTGCTTCCA GTGGTGAACA GACAATGACA ACAGACTCTA GTTTGAGAAC AGAGCAAAGA 
GACAAACCAT TGGTTGCTTC AGAGGACAAG ATTGGGGAAC ATGTCACTAA AACTGATTGC 
GCGAGCACTC CAAGTCCATC TACAGCATCC TGTAGATCCA GCTCTGAGGA ATCTACTAAA 
CCACTGCTGA AGGCGACCAG TGTTCAGGAA GATACTGAAA AGGGCAATGA ACAAGTGCAA 
GAAGAAAGTA TCTCAAGGTT CTCCAATGAC CCATTGGTTG CAGTTGATGC TCAACCAGAG 
AAAGAAGAGC AAGGCCTACA TTTGGCTTCG GTGACAAATG TTTCTTGCTG TGCCAGTCCA 
GAAAGAATCC CAGAGGAGCC CATGGAGGTT CCAGAGGAAG TTTCGGATAA GAGCCGAATC 
ACAGAAATTG ATGAGGCCAA AGTCTTGTCT TCAGAAGAAG GTGCAGTGAC TTCATCTGAA 
AGTAAGGCTG ACTCAGAAAC GCCAGACCCG TTTTCTCTTG ACGAGAAGCC TTTGAAATCG 
GACATTCTGG CTAATGTAGA ACAGGCTCAA AGCAGTGCTC CCATCCACCC TGATCATCAG 
GCAGAGCCAA CCAGTGGAGA GACCTCTGGA AGTTCCTCTC CGATATCAGT CATGGACAGA 
GATTCAGATA TATCTGGGGC CAGAGCCAAA GTCAGACTCC TTGAAGAAGA AGCTGACATT 
CAAGCGACCC ATCCAAGAAA ACGAAAAATG CCTAGAGCTT CTCCGCTAGC CCAAGCAAAT 
CTTACAGCTC AGCAGGTCAG AGAAAAGACA CAGCAGTCCC TCGCTGCGAT TGTAGATGCA 
CTGAAGCTTG AGGAGATTCA GCCTTACCAG ACAGAGAGAG CCAACCCATA TTATGAGTAC 
TTGCACATTC GCAAAAAGAT CGAAGAGAAG AGGAAAGTCC TTTGTAGTGT CATTCCTCAA 
GCGCCTCAGT ACTACGACGA GTATGTGACA TTCAATGGGT CCTATCTGCT GGATGGAAAC 
CCTCTCAGCA AACTCTGCAT CCCAACAATA ACGCCTCCAC CCTCTTTACC TGAACCACTG 
AAGGAGATGT TCAAACAGCA AGAGATGGTG CGGATGAAGC TGAGACTGCA GCACAGCATC 
GAAAGGGAAA AACTGATTGT ATCCAATGAG CAGGAGGTTT TGAGAGTTCA TTACCGCGCT 
GCGAGAACAC TAGCCAATCA GACGCTTCCG TTCAGTGCCT GCACAGTCCT ATTGGATGCC 
GAAGTATACA ACATGCCCCA AGAAGTCCAG GGTGATGATG GGAAGACTTC TGTTCGAGAT 
CGATTCAATG CGAGGCAGTT CATGTCTTGG CTTCAGGATG TAGACGACAA GTTTGACAAG 
TTGAAGACGT GTCTCCTGAT GCGGCAGCAG CATGAAGCGG CAGCGTTAAA CGCAGTCCAG 
CGTCTGGAGT GGCAGCTAAA GCTACAGGAA CTGGATCCCG CCACTTACAA GTCCACCAGC 
ATTTTCGAGA TTCCCGAATT CTACATCCCG CTCGTGGAGG TCAACGACGA CTTCGACCTG 
ACGCCGATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analyses of mRNA structure dynamics identify embryonic gene regulatory programs.
Nature structural & molecular biology25(8)677-686(2018 08)
Beaudoin JD,Novoa EM,Vejnar CE,Yartseva V,Takacs CM,Kellis M,Giraldez AJ

Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish.
PloS one13(6)e0197293(2018)
Grajevskaja V,Camerota D,Bellipanni G,Balciuniene J,Balciunas D

4.1Ba is necessary for glutamatergic synapse formation in the sensorimotor circuit of developing zebrafish.
PloS one13(10)e0205255(2018)
Fierro J Jr,Haynes DR,Washbourne P

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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ankrd12 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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