PRICKLE2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRICKLE2B
Entrez Gene ID	368250
Full Name	prickle homolog 2b
Synonyms	cb886,im:7138215,pk2,prickle2
General protein information	Preferred Names prickle homolog 2b Names prickle-like protein 2 prickle homolog 2 prickle-like 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_183343.1	NP_899186.1	prickle-like protein 2
XM_005155827.3	XP_005155884.1	prickle-like protein 2 isoform X1
XM_009305950.2	XP_009304225.1	prickle-like protein 2 isoform X2
XM_005155826.3	XP_005155883.1	prickle-like protein 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRICKLE2	NP_942559.1
Pan troglodytes (chimpanzee)	PRICKLE2	XP_001174646.1
Bos taurus (cattle)	PRICKLE2	XP_005222746.1
Mus musculus (house mouse)	Prickle2	NP_001074615.1
Danio rerio (zebrafish)	prickle2	NP_899186.1
Canis lupus familiaris (dog)	PRICKLE2	XP_005632405.1
Gallus gallus (chicken)	PRICKLE2	XP_001234704.3
Macaca mulatta (Rhesus monkey)	PRICKLE2	XP_001089576.2
Rattus norvegicus (Norway rat)	Prickle2	NP_001101346.1
Xenopus tropicalis (tropical clawed frog)	prickle2	NP_001103517.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional and evolutionary insights from the
Reeves WM, Wu Y, Harder MJ, Veeman MT
Development (Cambridge, England)144(18)3375-3387(2017 09)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Functional characterization of Prickle2 and BBS7 identify overlapping phenotypes yet distinct mechanisms.
Mei X, Westfall TA, Zhang Q, Sheffield VC, Bassuk AG, Slusarski DC
Developmental biology392(2)245-55(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRICKLE2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRICKLE2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03089	NM_183343.1 Latest version!	Danio rerio prickle homolog 2b (prickle2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa16979	XM_005155826.3 Latest version!	Danio rerio prickle homolog 2b (prickle2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa16980	XM_005155827.3 Latest version!	Danio rerio prickle homolog 2b (prickle2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa16980	XM_009305950.2 Latest version!	Danio rerio prickle homolog 2b (prickle2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRICKLE2B ( NM_183343.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_899186.1 Danio rerio prickle homolog 2b (prickle2b), mRNA.

Price & Availability↑

CloneID	ODa03089
Clone ID Related Accession (Same CDS sequence)	NM_183343.1
Accession Version	NM_183343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	prickle-like protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY278987.1. On Jul 6, 2005 this sequence version replaced XM_696920.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY278987.1, BC163236.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCTGG AGATGGAGAA GACGGTGACC AAACTGATGT ACGACTTCCA GAGGAACTCT 
ACATCTGACG ATGACTCTGG CTGTGCTCTT GAGGAGTATG CTTGGGTGCC ACCCGGACTC 
AAACCTGAAC AGGTCCATCA GTATTACAGC TCTTTGCCGG AAGATAAAGT ACCATACGTG 
AACAGTCCTG GAGAGAAGTA TCGCATCAAA CAGCTCCTGC ATCAGCTTCC ACCCCATGAC 
AATGAGGTGA GATTCTGCAA CAGTCTGGAT GATGAGGAGA AACGAGAGCT CAAGCTCTTC 
AGCAACCAGA GGAAGAGGGA GAACCTGGGC AGAGGAAACG TCAGACCCTT CCCTGTGACA 
ATGACCGGAG CCATCTGTGA ACAGTGTGGA GGACAGATCA ATGGAGGTGA CATTGCGGTC 
TTTGCATCAC GAGCAGGTCA TGGTGTTTGC TGGCACCCCC AGTGCTTTGT GTGCAGCATG 
TGTGATGAGC TGCTGGTGGA TCTGATCTAC TTCTACCAGG ACGGGAAGAT CTTCTGCGGT 
CGCCATCATG CAGAGAGACT CAAACCCCGC TGTTCAGCCT GTGATGAGAT TATTTTAGCA 
GACGAGTGCA CAGAGGCCGA GGGCAGGCAC TGGCACATGA AGCACTTCTG CTGTTTCGAG 
TGCGAGACAG TTCTGGGCGG CCAGCGGTAC ATCATGAAGG AGGGTCGGCC CTACTGTTGC 
ACCTGCTTCG AGTCCTTGTA TGCCGAATAC TGCGACTCCT GCGGGGAACA CATCGGTATT 
GACCAGGGCC AGATGACGTA TGATGGTCAG CACTGGCATG CCACAGAGGC TTGTTTCAGC 
TGTGCTCGCT GTAAGAAGTC TCTGCTCGGT CGTCCTTTTC TACCCAAGCA GGGTCAGATC 
TTCTGCTCTC GGGCTTGTAG TGTCGGCGAT GAGCAAAACG GCTCTGATTC CTCAGATTCT 
GCATTTCAGA GCGCCCGTTC ACGTGAAGCC CGTCGCAGCA GCTCCAAATC CAACAAGAGC 
AGTACTGGAG GAAGTGGAGG AGATGGACAC CAGAGTGGGA AAGCTTCAGC TATGCGTTTC 
TCTGCTGATG TTGACCCACT GTCTCTGCAA ATGGATTTGC TCAGTCTGTC CAGTCAAACT 
CCTAGCTTAA ATCGAGAATC TCCATCCTGG AAGACGCAGG CTGAGATGTT TGGATATGAG 
GGCCGTAATG AACTCTCCAC AAATCCTCTT CATTTGCTCA GTCAGTGCAA CATCCGGACT 
TCCTATTCCT CAAATCTGTC CCCTGGTCAC CTATCAGATC CAAGGCCCAA GGAGCCTGCG 
TCCTCTAAGA GACCCCCTGT ATCTGCTCTA AAAGGGAAGT CTTTGAATGA GAACTGGTTC 
CAGCAGGGAC CAGAGGACTA TTATCCACCT GCACTGCGAA CCCAAGCGAG CTTTAACGAG 
GTTCCACACA ACAGCTTCAT GGATAAGCGC TCTGTAAGTC TGCATGTGTT CCAGAGGGAG 
AAGGAAAAGG ATGTTGGGCC ACAGATGAGC CGCAGCAGGA ACCCCATCAG TGCACTTGGC 
TTTAGTGAAC AGCTCACACC ACTGGAACAA ACACCACGCG GCTCTATGGA GTCACTGGCT 
CTATCTAATG CTACAGGCAC CTCTGCAGAT GGCGGCAGTA AAAGGCAGGA ACACTTGTCT 
CGCTTCTCAA TGCCGGACTT GAGTAAAGAT TCTGGCATGA ACGTGTCTGA AAAGAGCAAC 
ATGGGCACCC TCAACTCCTC TGTGCAGTTC CACAGCTCTG AATCTCTGCG CAGCCTGAAC 
TCGGCTCAGC CCTACCTGGA GCTCGAAGCT CCGGTTCAAG TCAGGTTTCC TGTGCAATAC 
CCCCGAGAAC CTTCTGGAAT TAATGCTTTG CCGCCTGGTT TTGCCTATCA GGAGGAAGAT 
CGCGTAAGTT TGGTTAGCAA CGCCAATAAT GCTCGTCTAC CACCAATGAG CGAACGCACC 
CGCCGTCGTA GTATAGATCA CGAAGAGCCA CGCAGACGAC ACCACCACCA TCGCTCCCGC 
CGCTCCAGGC GATCCCGTTC AGACAATGCA CTCAACTTAG TCGCTGAACG CCGCCCAGCT 
CTGAAACGAT CTCAACTTCA CGCTCGTGAG GATTACGACC AGTTTCCTCT TCCCCGTGGA 
TCTCGGGACA CTTACGGAAG TGGCAGACTG AGGCAACAGC CTTTCAGACC TTGCCCTCGA 
ACAACCTCTG ACCTCACGCT TCAAAACCCC GCTCTCCACC GACACCAGGG TCCTTATTCG 
TGGGATCAGT ATGATTACGA TGACGACTGG TGCTCCACAT GTTCCTCGTC TTCCGAGTCA 
GAAGATGAAG GGTATTTTCT GGGTGAGCCA ATTCCCAGAC CCATCCAACT GAGGCACATG 
ACTAGCGAAG AGCTGCTACG CAAATACAAC TCTTCAGGAC TCGGAGCATC CGGTCAATTC 
GGCAGTCGAG GACAGTTACA TATGCGCAAA CGTAGGAAGA GCAAGAACTG CATTATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899186.1
CDS	85..2607
Translation	MPLEMEKTVTKLMYDFQRNSTSDDDSGCALEEYAWVPPGLKPEQVHQYYSSLPEDKVPYVNSPGEKYRIKQLLHQLPPHDNEVRFCNSLDDEEKRELKLFSNQRKRENLGRGNVRPFPVTMTGAICEQCGGQINGGDIAVFASRAGHGVCWHPQCFVCSMCDELLVDLIYFYQDGKIFCGRHHAERLKPRCSACDEIILADECTEAEGRHWHMKHFCCFECETVLGGQRYIMKEGRPYCCTCFESLYAEYCDSCGEHIGIDQGQMTYDGQHWHATEACFSCARCKKSLLGRPFLPKQGQIFCSRACSVGDEQNGSDSSDSAFQSARSREARRSSSKSNKSSTGGSGGDGHQSGKASAMRFSADVDPLSLQMDLLSLSSQTPSLNRESPSWKTQAEMFGYEGRNELSTNPLHLLSQCNIRTSYSSNLSPGHLSDPRPKEPASSKRPPVSALKGKSLNENWFQQGPEDYYPPALRTQASFNEVPHNSFMDKRSVSLHVFQREKEKDVGPQMSRSRNPISALGFSEQLTPLEQTPRGSMESLALSNATGTSADGGSKRQEHLSRFSMPDLSKDSGMNVSEKSNMGTLNSSVQFHSSESLRSLNSAQPYLELEAPVQVRFPVQYPREPSGINALPPGFAYQEEDRVSLVSNANNARLPPMSERTRRRSIDHEEPRRRHHHHRSRRSRRSRSDNALNLVAERRPALKRSQLHAREDYDQFPLPRGSRDTYGSGRLRQQPFRPCPRTTSDLTLQNPALHRHQGPYSWDQYDYDDDWCSTCSSSSESEDEGYFLGEPIPRPIQLRHMTSEELLRKYNSSGLGASGQFGSRGQLHMRKRRKSKNCIIS

Target ORF information:

RefSeq Version	NM_183343.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prickle homolog 2b (prickle2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183343.1

ORF Insert Sequence:

ATGCCTCTGG AGATGGAGAA GACGGTGACC AAACTGATGT ACGACTTCCA GAGGAACTCT 
ACATCTGACG ATGACTCTGG CTGTGCTCTT GAGGAGTATG CTTGGGTGCC ACCCGGACTC 
AAACCTGAAC AGGTCCATCA GTATTACAGC TCTTTGCCGG AAGATAAAGT ACCATACGTG 
AACAGTCCTG GAGAGAAGTA TCGCATCAAA CAGCTCCTGC ATCAGCTTCC ACCCCATGAC 
AATGAGGTGA GATTCTGCAA CAGTCTGGAT GATGAGGAGA AACGAGAGCT CAAGCTCTTC 
AGCAACCAGA GGAAGAGGGA GAACCTGGGC AGAGGAAACG TCAGACCCTT CCCTGTGACA 
ATGACCGGAG CCATCTGTGA ACAGTGTGGA GGACAGATCA ATGGAGGTGA CATTGCGGTC 
TTTGCATCAC GAGCAGGTCA TGGTGTTTGC TGGCACCCCC AGTGCTTTGT GTGCAGCATG 
TGTGATGAGC TGCTGGTGGA TCTGATCTAC TTCTACCAGG ACGGGAAGAT CTTCTGCGGT 
CGCCATCATG CAGAGAGACT CAAACCCCGC TGTTCAGCCT GTGATGAGAT TATTTTAGCA 
GACGAGTGCA CAGAGGCCGA GGGCAGGCAC TGGCACATGA AGCACTTCTG CTGTTTCGAG 
TGCGAGACAG TTCTGGGCGG CCAGCGGTAC ATCATGAAGG AGGGTCGGCC CTACTGTTGC 
ACCTGCTTCG AGTCCTTGTA TGCCGAATAC TGCGACTCCT GCGGGGAACA CATCGGTATT 
GACCAGGGCC AGATGACGTA TGATGGTCAG CACTGGCATG CCACAGAGGC TTGTTTCAGC 
TGTGCTCGCT GTAAGAAGTC TCTGCTCGGT CGTCCTTTTC TACCCAAGCA GGGTCAGATC 
TTCTGCTCTC GGGCTTGTAG TGTCGGCGAT GAGCAAAACG GCTCTGATTC CTCAGATTCT 
GCATTTCAGA GCGCCCGTTC ACGTGAAGCC CGTCGCAGCA GCTCCAAATC CAACAAGAGC 
AGTACTGGAG GAAGTGGAGG AGATGGACAC CAGAGTGGGA AAGCTTCAGC TATGCGTTTC 
TCTGCTGATG TTGACCCACT GTCTCTGCAA ATGGATTTGC TCAGTCTGTC CAGTCAAACT 
CCTAGCTTAA ATCGAGAATC TCCATCCTGG AAGACGCAGG CTGAGATGTT TGGATATGAG 
GGCCGTAATG AACTCTCCAC AAATCCTCTT CATTTGCTCA GTCAGTGCAA CATCCGGACT 
TCCTATTCCT CAAATCTGTC CCCTGGTCAC CTATCAGATC CAAGGCCCAA GGAGCCTGCG 
TCCTCTAAGA GACCCCCTGT ATCTGCTCTA AAAGGGAAGT CTTTGAATGA GAACTGGTTC 
CAGCAGGGAC CAGAGGACTA TTATCCACCT GCACTGCGAA CCCAAGCGAG CTTTAACGAG 
GTTCCACACA ACAGCTTCAT GGATAAGCGC TCTGTAAGTC TGCATGTGTT CCAGAGGGAG 
AAGGAAAAGG ATGTTGGGCC ACAGATGAGC CGCAGCAGGA ACCCCATCAG TGCACTTGGC 
TTTAGTGAAC AGCTCACACC ACTGGAACAA ACACCACGCG GCTCTATGGA GTCACTGGCT 
CTATCTAATG CTACAGGCAC CTCTGCAGAT GGCGGCAGTA AAAGGCAGGA ACACTTGTCT 
CGCTTCTCAA TGCCGGACTT GAGTAAAGAT TCTGGCATGA ACGTGTCTGA AAAGAGCAAC 
ATGGGCACCC TCAACTCCTC TGTGCAGTTC CACAGCTCTG AATCTCTGCG CAGCCTGAAC 
TCGGCTCAGC CCTACCTGGA GCTCGAAGCT CCGGTTCAAG TCAGGTTTCC TGTGCAATAC 
CCCCGAGAAC CTTCTGGAAT TAATGCTTTG CCGCCTGGTT TTGCCTATCA GGAGGAAGAT 
CGCGTAAGTT TGGTTAGCAA CGCCAATAAT GCTCGTCTAC CACCAATGAG CGAACGCACC 
CGCCGTCGTA GTATAGATCA CGAAGAGCCA CGCAGACGAC ACCACCACCA TCGCTCCCGC 
CGCTCCAGGC GATCCCGTTC AGACAATGCA CTCAACTTAG TCGCTGAACG CCGCCCAGCT 
CTGAAACGAT CTCAACTTCA CGCTCGTGAG GATTACGACC AGTTTCCTCT TCCCCGTGGA 
TCTCGGGACA CTTACGGAAG TGGCAGACTG AGGCAACAGC CTTTCAGACC TTGCCCTCGA 
ACAACCTCTG ACCTCACGCT TCAAAACCCC GCTCTCCACC GACACCAGGG TCCTTATTCG 
TGGGATCAGT ATGATTACGA TGACGACTGG TGCTCCACAT GTTCCTCGTC TTCCGAGTCA 
GAAGATGAAG GGTATTTTCT GGGTGAGCCA ATTCCCAGAC CCATCCAACT GAGGCACATG 
ACTAGCGAAG AGCTGCTACG CAAATACAAC TCTTCAGGAC TCGGAGCATC CGGTCAATTC 
GGCAGTCGAG GACAGTTACA TATGCGCAAA CGTAGGAAGA GCAAGAACTG CATTATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRICKLE2B ( XM_005155826.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155883.1 Danio rerio prickle homolog 2b (prickle2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16979
Clone ID Related Accession (Same CDS sequence)	XM_005155826.3
Accession Version	XM_005155826.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	prickle-like protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155826.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTTC GAAGCCTTAA AAAGAGAAAC TCCTGCCGGT CGCCCACTGT GCTTGATGAC 
CTAGAAAGAG GACAGCCATG CAACGTGTGT GGAGAGCAGT GTCCAGGCTT CATTCTGCAT 
AAATGGAGGA AAATCTGTCT CCACTGTAAG TGCCCTCGTG AGGAGCATGT TGTGACTGCC 
ATGCCTCTGG AGATGGAGAA GACGGTGACC AAACTGATGT ACGACTTCCA GAGGAACTCT 
ACATCTGACG ATGACTCTGG CTGTGCTCTT GAGGAGTATG CTTGGGTGCC ACCCGGACTC 
AAACCTGAAC AGGTCCATCA GTATTACAGC TCTTTGCCGG AAGATAAAGT ACCATACGTG 
AACAGTCCTG GAGAGAAGTA TCGCATCAAA CAGCTCCTGC ATCAGCTTCC ACCCCATGAC 
AATGAGGTGA GATACTGCAA CAGTCTGGAT GATGAGGAGA AACGAGAGCT CAAGCTCTTC 
AGCAACCAGA GGAAGAGGGA GAACCTGGGC AGAGGAAACG TCAGACCCTT CCCCGTGACA 
ATGACCGGAG CCATCTGTGA ACAGTGTGGA GGACAGATCA ATGGAGGTGA CATTGCGGTC 
TTTGCATCAC GAGCAGGTCA TGGTGTTTGC TGGCACCCCC AGTGCTTTGT GTGCAGCATG 
TGTGATGAGC TGCTGGTGGA TCTGATCTAC TTCTACCAGG ACGGGAAGAT CTTCTGCGGT 
CGCCATCATG CAGAGAGACT CAAACCCCGC TGTTCAGCCT GTGATGAGAT TATTTTAGCA 
GACGAGTGCA CAGAGGCCGA GGGCAGGCAC TGGCACATGA AGCACTTCTG CTGTTTCGAG 
TGCGAGACAG TTCTGGGCGG CCAGCGCTAC ATCATGAAGG AGGGTCGGCC CTACTGTTGC 
ACCTGCTTCG AGTCCTTGTA TGCCGAATAC TGCGACTCCT GCGGGGAACA CATCGGTATT 
GACCAGGGCC AGATGACGTA TGATGGTCAG CACTGGCATG CCACAGAGGC TTGTTTCAGC 
TGTGCTCGCT GTAAGAAGTC TCTGCTCGGT CGTCCTTTTC TACCCAAGCA GGGTCAGATC 
TTCTGCTCTC GGGCTTGTAG TGTCGGCGAT GACCAAAACG GCTCTGATTC CTCAGATTCT 
GCATTTCAGA GCGCCCGTTC ACGTGAAGCC CGTCGCAGCA GCTCCAAATC CAACAAGAGC 
AGTACTGGAG GAAGTGGAGG AGATGGACAC CAGAGTGGGA AAGCTTCAGC TATGCGTTTC 
TCTGCTGATG TTGACCCACT GTCTCTGCAA ATGGATTTGC TCAGTCTGTC CAGTCAAACT 
CCTAGCTTAA ATCGAGAATC TCCATCCTGG AAGACGCAGG CTGAGATGTT TGGATATGAG 
GGTCGTAATG AACTCTCCAC AAATCCTCTT CATTTGCTCA GTCAGTGCAA CATTCGGACT 
TCCTATTCCT CAAATCTGTC CCCTGGTCAC CTATCAGATC CAAGGCCCAA GGAGCCTGCG 
TCTTCTAAGA GACCCCCTGT ATCTGCTCTA AAAGGGAAGT CTTTGAATGA GAACTGGTTC 
CAGCAGGGAC CAGAGGACTA TTATCCACCT GCACTGCGAA CCCAAGCGAG CTTTAACGAG 
GTTCCACACA ACAGCTTCAT GGATAAGCGC TCTGTAAGTC TGCATGTGTT CCAGAGGGAG 
AAGGAAAAGG ATGTTGGGCC ACAGATGAGC CGCAGCAGGA ACCCCATCAG TGCACTTGGC 
TTTAGTGAAC AGCTCACACC ACTGGAACAA ACACCACGCG GCTCTATGGA GTCACTGGCT 
CTATCTAATG CTACAGGCAC CTCTGCAGAT GGCGGCAGTA AAAGGCAGGA GCACTTGTCT 
CGCTTCTCAA TGCCGGACTT GAGTAAAGAT TCTGGCATGA ACGTGTCTGA AAAGAGCAAC 
ATGGGCACCC TCAACTCCTC TGTGCAGTTC CACAGCTCTG AATCTCTGCG CAGCCTGAAC 
TCGGCTCAGC CCTACCTGGA GCTCGAAGCT CCGGTCCAAG TCAGGTTTCC TGTGCAATAC 
CCCCGAGAAC CTTCTGGAAT TAATGCTTTG CCGCCTGGTT TTGCCTATCA GGAGGAAGAT 
CGCGTAAGTT TGGTTAGCAA CGCCAATAAT GCTCGTCTAC CACCAATGAG CGAACGCACC 
CGCCGTCGTA GTATAGATCA CGAAGAGCCA CGCAGACGAC ACCACCACCA TCGCTCCCGC 
CGCTCCAGGC GATCCCGTTC AGACAATGCA CTCAACTTAG TCGCTGAACG CCGCCCAGCT 
CCGAAACGAT CTCAACTTCA TGCTCGTGAG GATTACGACC AGTTCCCTCT TCCCCGTGGA 
TCTCGGGACA CTTACGGAAG TGGCAGACTG AGGCAACAGC CTTTCAGACC TTGCCCTCGA 
ACAACCTCTG ACCTCACGCT TCAAAACCCC GCTCTCCACC GACACCAGGG TCCTTATTCG 
TGGGATCAGT ATGATTACAA TGACGACTGG TGCTCCACAT GTTCCTCATC TTCCGAGTCA 
GAAGATGAAG GGTATTTTCT GGGTGAGCCA ATTCCCAGAC CCATCCAACT GAGGCACATG 
ACTAGCGAAG AGCTGCTACG CAAATACAAC TCTTCAGGAC TCGGAGCATC CGGTCAATTC 
GGCAGTCGAG GACAGTTACA TATGCGCAAA CGTAGGAAGA GCAAGAACTG CATTATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155883.1
CDS	53..2755
Translation	MFVRSLKKRNSCRSPTVLDDLERGQPCNVCGEQCPGFILHKWRKICLHCKCPREEHVVTAMPLEMEKTVTKLMYDFQRNSTSDDDSGCALEEYAWVPPGLKPEQVHQYYSSLPEDKVPYVNSPGEKYRIKQLLHQLPPHDNEVRYCNSLDDEEKRELKLFSNQRKRENLGRGNVRPFPVTMTGAICEQCGGQINGGDIAVFASRAGHGVCWHPQCFVCSMCDELLVDLIYFYQDGKIFCGRHHAERLKPRCSACDEIILADECTEAEGRHWHMKHFCCFECETVLGGQRYIMKEGRPYCCTCFESLYAEYCDSCGEHIGIDQGQMTYDGQHWHATEACFSCARCKKSLLGRPFLPKQGQIFCSRACSVGDDQNGSDSSDSAFQSARSREARRSSSKSNKSSTGGSGGDGHQSGKASAMRFSADVDPLSLQMDLLSLSSQTPSLNRESPSWKTQAEMFGYEGRNELSTNPLHLLSQCNIRTSYSSNLSPGHLSDPRPKEPASSKRPPVSALKGKSLNENWFQQGPEDYYPPALRTQASFNEVPHNSFMDKRSVSLHVFQREKEKDVGPQMSRSRNPISALGFSEQLTPLEQTPRGSMESLALSNATGTSADGGSKRQEHLSRFSMPDLSKDSGMNVSEKSNMGTLNSSVQFHSSESLRSLNSAQPYLELEAPVQVRFPVQYPREPSGINALPPGFAYQEEDRVSLVSNANNARLPPMSERTRRRSIDHEEPRRRHHHHRSRRSRRSRSDNALNLVAERRPAPKRSQLHAREDYDQFPLPRGSRDTYGSGRLRQQPFRPCPRTTSDLTLQNPALHRHQGPYSWDQYDYNDDWCSTCSSSSESEDEGYFLGEPIPRPIQLRHMTSEELLRKYNSSGLGASGQFGSRGQLHMRKRRKSKNCIIS

Target ORF information:

RefSeq Version	XM_005155826.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio prickle homolog 2b (prickle2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155826.3

ORF Insert Sequence:

ATGTTTGTTC GAAGCCTTAA AAAGAGAAAC TCCTGCCGGT CGCCCACTGT GCTTGATGAC 
CTAGAAAGAG GACAGCCATG CAACGTGTGT GGAGAGCAGT GTCCAGGCTT CATTCTGCAT 
AAATGGAGGA AAATCTGTCT CCACTGTAAG TGCCCTCGTG AGGAGCATGT TGTGACTGCC 
ATGCCTCTGG AGATGGAGAA GACGGTGACC AAACTGATGT ACGACTTCCA GAGGAACTCT 
ACATCTGACG ATGACTCTGG CTGTGCTCTT GAGGAGTATG CTTGGGTGCC ACCCGGACTC 
AAACCTGAAC AGGTCCATCA GTATTACAGC TCTTTGCCGG AAGATAAAGT ACCATACGTG 
AACAGTCCTG GAGAGAAGTA TCGCATCAAA CAGCTCCTGC ATCAGCTTCC ACCCCATGAC 
AATGAGGTGA GATACTGCAA CAGTCTGGAT GATGAGGAGA AACGAGAGCT CAAGCTCTTC 
AGCAACCAGA GGAAGAGGGA GAACCTGGGC AGAGGAAACG TCAGACCCTT CCCCGTGACA 
ATGACCGGAG CCATCTGTGA ACAGTGTGGA GGACAGATCA ATGGAGGTGA CATTGCGGTC 
TTTGCATCAC GAGCAGGTCA TGGTGTTTGC TGGCACCCCC AGTGCTTTGT GTGCAGCATG 
TGTGATGAGC TGCTGGTGGA TCTGATCTAC TTCTACCAGG ACGGGAAGAT CTTCTGCGGT 
CGCCATCATG CAGAGAGACT CAAACCCCGC TGTTCAGCCT GTGATGAGAT TATTTTAGCA 
GACGAGTGCA CAGAGGCCGA GGGCAGGCAC TGGCACATGA AGCACTTCTG CTGTTTCGAG 
TGCGAGACAG TTCTGGGCGG CCAGCGCTAC ATCATGAAGG AGGGTCGGCC CTACTGTTGC 
ACCTGCTTCG AGTCCTTGTA TGCCGAATAC TGCGACTCCT GCGGGGAACA CATCGGTATT 
GACCAGGGCC AGATGACGTA TGATGGTCAG CACTGGCATG CCACAGAGGC TTGTTTCAGC 
TGTGCTCGCT GTAAGAAGTC TCTGCTCGGT CGTCCTTTTC TACCCAAGCA GGGTCAGATC 
TTCTGCTCTC GGGCTTGTAG TGTCGGCGAT GACCAAAACG GCTCTGATTC CTCAGATTCT 
GCATTTCAGA GCGCCCGTTC ACGTGAAGCC CGTCGCAGCA GCTCCAAATC CAACAAGAGC 
AGTACTGGAG GAAGTGGAGG AGATGGACAC CAGAGTGGGA AAGCTTCAGC TATGCGTTTC 
TCTGCTGATG TTGACCCACT GTCTCTGCAA ATGGATTTGC TCAGTCTGTC CAGTCAAACT 
CCTAGCTTAA ATCGAGAATC TCCATCCTGG AAGACGCAGG CTGAGATGTT TGGATATGAG 
GGTCGTAATG AACTCTCCAC AAATCCTCTT CATTTGCTCA GTCAGTGCAA CATTCGGACT 
TCCTATTCCT CAAATCTGTC CCCTGGTCAC CTATCAGATC CAAGGCCCAA GGAGCCTGCG 
TCTTCTAAGA GACCCCCTGT ATCTGCTCTA AAAGGGAAGT CTTTGAATGA GAACTGGTTC 
CAGCAGGGAC CAGAGGACTA TTATCCACCT GCACTGCGAA CCCAAGCGAG CTTTAACGAG 
GTTCCACACA ACAGCTTCAT GGATAAGCGC TCTGTAAGTC TGCATGTGTT CCAGAGGGAG 
AAGGAAAAGG ATGTTGGGCC ACAGATGAGC CGCAGCAGGA ACCCCATCAG TGCACTTGGC 
TTTAGTGAAC AGCTCACACC ACTGGAACAA ACACCACGCG GCTCTATGGA GTCACTGGCT 
CTATCTAATG CTACAGGCAC CTCTGCAGAT GGCGGCAGTA AAAGGCAGGA GCACTTGTCT 
CGCTTCTCAA TGCCGGACTT GAGTAAAGAT TCTGGCATGA ACGTGTCTGA AAAGAGCAAC 
ATGGGCACCC TCAACTCCTC TGTGCAGTTC CACAGCTCTG AATCTCTGCG CAGCCTGAAC 
TCGGCTCAGC CCTACCTGGA GCTCGAAGCT CCGGTCCAAG TCAGGTTTCC TGTGCAATAC 
CCCCGAGAAC CTTCTGGAAT TAATGCTTTG CCGCCTGGTT TTGCCTATCA GGAGGAAGAT 
CGCGTAAGTT TGGTTAGCAA CGCCAATAAT GCTCGTCTAC CACCAATGAG CGAACGCACC 
CGCCGTCGTA GTATAGATCA CGAAGAGCCA CGCAGACGAC ACCACCACCA TCGCTCCCGC 
CGCTCCAGGC GATCCCGTTC AGACAATGCA CTCAACTTAG TCGCTGAACG CCGCCCAGCT 
CCGAAACGAT CTCAACTTCA TGCTCGTGAG GATTACGACC AGTTCCCTCT TCCCCGTGGA 
TCTCGGGACA CTTACGGAAG TGGCAGACTG AGGCAACAGC CTTTCAGACC TTGCCCTCGA 
ACAACCTCTG ACCTCACGCT TCAAAACCCC GCTCTCCACC GACACCAGGG TCCTTATTCG 
TGGGATCAGT ATGATTACAA TGACGACTGG TGCTCCACAT GTTCCTCATC TTCCGAGTCA 
GAAGATGAAG GGTATTTTCT GGGTGAGCCA ATTCCCAGAC CCATCCAACT GAGGCACATG 
ACTAGCGAAG AGCTGCTACG CAAATACAAC TCTTCAGGAC TCGGAGCATC CGGTCAATTC 
GGCAGTCGAG GACAGTTACA TATGCGCAAA CGTAGGAAGA GCAAGAACTG CATTATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRICKLE2B ( XM_005155827.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155884.1 Danio rerio prickle homolog 2b (prickle2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16980
Clone ID Related Accession (Same CDS sequence)	XM_005155827.3 , XM_009305950.2
Accession Version	XM_005155827.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	prickle-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTGG AGATGGAGAA GACGGTGACC AAACTGATGT ACGACTTCCA GAGGAACTCT 
ACATCTGACG ATGACTCTGG CTGTGCTCTT GAGGAGTATG CTTGGGTGCC ACCCGGACTC 
AAACCTGAAC AGGTCCATCA GTATTACAGC TCTTTGCCGG AAGATAAAGT ACCATACGTG 
AACAGTCCTG GAGAGAAGTA TCGCATCAAA CAGCTCCTGC ATCAGCTTCC ACCCCATGAC 
AATGAGGTGA GATACTGCAA CAGTCTGGAT GATGAGGAGA AACGAGAGCT CAAGCTCTTC 
AGCAACCAGA GGAAGAGGGA GAACCTGGGC AGAGGAAACG TCAGACCCTT CCCCGTGACA 
ATGACCGGAG CCATCTGTGA ACAGTGTGGA GGACAGATCA ATGGAGGTGA CATTGCGGTC 
TTTGCATCAC GAGCAGGTCA TGGTGTTTGC TGGCACCCCC AGTGCTTTGT GTGCAGCATG 
TGTGATGAGC TGCTGGTGGA TCTGATCTAC TTCTACCAGG ACGGGAAGAT CTTCTGCGGT 
CGCCATCATG CAGAGAGACT CAAACCCCGC TGTTCAGCCT GTGATGAGAT TATTTTAGCA 
GACGAGTGCA CAGAGGCCGA GGGCAGGCAC TGGCACATGA AGCACTTCTG CTGTTTCGAG 
TGCGAGACAG TTCTGGGCGG CCAGCGCTAC ATCATGAAGG AGGGTCGGCC CTACTGTTGC 
ACCTGCTTCG AGTCCTTGTA TGCCGAATAC TGCGACTCCT GCGGGGAACA CATCGGTATT 
GACCAGGGCC AGATGACGTA TGATGGTCAG CACTGGCATG CCACAGAGGC TTGTTTCAGC 
TGTGCTCGCT GTAAGAAGTC TCTGCTCGGT CGTCCTTTTC TACCCAAGCA GGGTCAGATC 
TTCTGCTCTC GGGCTTGTAG TGTCGGCGAT GACCAAAACG GCTCTGATTC CTCAGATTCT 
GCATTTCAGA GCGCCCGTTC ACGTGAAGCC CGTCGCAGCA GCTCCAAATC CAACAAGAGC 
AGTACTGGAG GAAGTGGAGG AGATGGACAC CAGAGTGGGA AAGCTTCAGC TATGCGTTTC 
TCTGCTGATG TTGACCCACT GTCTCTGCAA ATGGATTTGC TCAGTCTGTC CAGTCAAACT 
CCTAGCTTAA ATCGAGAATC TCCATCCTGG AAGACGCAGG CTGAGATGTT TGGATATGAG 
GGTCGTAATG AACTCTCCAC AAATCCTCTT CATTTGCTCA GTCAGTGCAA CATTCGGACT 
TCCTATTCCT CAAATCTGTC CCCTGGTCAC CTATCAGATC CAAGGCCCAA GGAGCCTGCG 
TCTTCTAAGA GACCCCCTGT ATCTGCTCTA AAAGGGAAGT CTTTGAATGA GAACTGGTTC 
CAGCAGGGAC CAGAGGACTA TTATCCACCT GCACTGCGAA CCCAAGCGAG CTTTAACGAG 
GTTCCACACA ACAGCTTCAT GGATAAGCGC TCTGTAAGTC TGCATGTGTT CCAGAGGGAG 
AAGGAAAAGG ATGTTGGGCC ACAGATGAGC CGCAGCAGGA ACCCCATCAG TGCACTTGGC 
TTTAGTGAAC AGCTCACACC ACTGGAACAA ACACCACGCG GCTCTATGGA GTCACTGGCT 
CTATCTAATG CTACAGGCAC CTCTGCAGAT GGCGGCAGTA AAAGGCAGGA GCACTTGTCT 
CGCTTCTCAA TGCCGGACTT GAGTAAAGAT TCTGGCATGA ACGTGTCTGA AAAGAGCAAC 
ATGGGCACCC TCAACTCCTC TGTGCAGTTC CACAGCTCTG AATCTCTGCG CAGCCTGAAC 
TCGGCTCAGC CCTACCTGGA GCTCGAAGCT CCGGTCCAAG TCAGGTTTCC TGTGCAATAC 
CCCCGAGAAC CTTCTGGAAT TAATGCTTTG CCGCCTGGTT TTGCCTATCA GGAGGAAGAT 
CGCGTAAGTT TGGTTAGCAA CGCCAATAAT GCTCGTCTAC CACCAATGAG CGAACGCACC 
CGCCGTCGTA GTATAGATCA CGAAGAGCCA CGCAGACGAC ACCACCACCA TCGCTCCCGC 
CGCTCCAGGC GATCCCGTTC AGACAATGCA CTCAACTTAG TCGCTGAACG CCGCCCAGCT 
CCGAAACGAT CTCAACTTCA TGCTCGTGAG GATTACGACC AGTTCCCTCT TCCCCGTGGA 
TCTCGGGACA CTTACGGAAG TGGCAGACTG AGGCAACAGC CTTTCAGACC TTGCCCTCGA 
ACAACCTCTG ACCTCACGCT TCAAAACCCC GCTCTCCACC GACACCAGGG TCCTTATTCG 
TGGGATCAGT ATGATTACAA TGACGACTGG TGCTCCACAT GTTCCTCATC TTCCGAGTCA 
GAAGATGAAG GGTATTTTCT GGGTGAGCCA ATTCCCAGAC CCATCCAACT GAGGCACATG 
ACTAGCGAAG AGCTGCTACG CAAATACAAC TCTTCAGGAC TCGGAGCATC CGGTCAATTC 
GGCAGTCGAG GACAGTTACA TATGCGCAAA CGTAGGAAGA GCAAGAACTG CATTATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155884.1
CDS	401..2923
Translation	MPLEMEKTVTKLMYDFQRNSTSDDDSGCALEEYAWVPPGLKPEQVHQYYSSLPEDKVPYVNSPGEKYRIKQLLHQLPPHDNEVRYCNSLDDEEKRELKLFSNQRKRENLGRGNVRPFPVTMTGAICEQCGGQINGGDIAVFASRAGHGVCWHPQCFVCSMCDELLVDLIYFYQDGKIFCGRHHAERLKPRCSACDEIILADECTEAEGRHWHMKHFCCFECETVLGGQRYIMKEGRPYCCTCFESLYAEYCDSCGEHIGIDQGQMTYDGQHWHATEACFSCARCKKSLLGRPFLPKQGQIFCSRACSVGDDQNGSDSSDSAFQSARSREARRSSSKSNKSSTGGSGGDGHQSGKASAMRFSADVDPLSLQMDLLSLSSQTPSLNRESPSWKTQAEMFGYEGRNELSTNPLHLLSQCNIRTSYSSNLSPGHLSDPRPKEPASSKRPPVSALKGKSLNENWFQQGPEDYYPPALRTQASFNEVPHNSFMDKRSVSLHVFQREKEKDVGPQMSRSRNPISALGFSEQLTPLEQTPRGSMESLALSNATGTSADGGSKRQEHLSRFSMPDLSKDSGMNVSEKSNMGTLNSSVQFHSSESLRSLNSAQPYLELEAPVQVRFPVQYPREPSGINALPPGFAYQEEDRVSLVSNANNARLPPMSERTRRRSIDHEEPRRRHHHHRSRRSRRSRSDNALNLVAERRPAPKRSQLHAREDYDQFPLPRGSRDTYGSGRLRQQPFRPCPRTTSDLTLQNPALHRHQGPYSWDQYDYNDDWCSTCSSSSESEDEGYFLGEPIPRPIQLRHMTSEELLRKYNSSGLGASGQFGSRGQLHMRKRRKSKNCIIS

Target ORF information:

RefSeq Version	XM_005155827.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prickle homolog 2b (prickle2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155827.3

ORF Insert Sequence:

ATGCCTCTGG AGATGGAGAA GACGGTGACC AAACTGATGT ACGACTTCCA GAGGAACTCT 
ACATCTGACG ATGACTCTGG CTGTGCTCTT GAGGAGTATG CTTGGGTGCC ACCCGGACTC 
AAACCTGAAC AGGTCCATCA GTATTACAGC TCTTTGCCGG AAGATAAAGT ACCATACGTG 
AACAGTCCTG GAGAGAAGTA TCGCATCAAA CAGCTCCTGC ATCAGCTTCC ACCCCATGAC 
AATGAGGTGA GATACTGCAA CAGTCTGGAT GATGAGGAGA AACGAGAGCT CAAGCTCTTC 
AGCAACCAGA GGAAGAGGGA GAACCTGGGC AGAGGAAACG TCAGACCCTT CCCCGTGACA 
ATGACCGGAG CCATCTGTGA ACAGTGTGGA GGACAGATCA ATGGAGGTGA CATTGCGGTC 
TTTGCATCAC GAGCAGGTCA TGGTGTTTGC TGGCACCCCC AGTGCTTTGT GTGCAGCATG 
TGTGATGAGC TGCTGGTGGA TCTGATCTAC TTCTACCAGG ACGGGAAGAT CTTCTGCGGT 
CGCCATCATG CAGAGAGACT CAAACCCCGC TGTTCAGCCT GTGATGAGAT TATTTTAGCA 
GACGAGTGCA CAGAGGCCGA GGGCAGGCAC TGGCACATGA AGCACTTCTG CTGTTTCGAG 
TGCGAGACAG TTCTGGGCGG CCAGCGCTAC ATCATGAAGG AGGGTCGGCC CTACTGTTGC 
ACCTGCTTCG AGTCCTTGTA TGCCGAATAC TGCGACTCCT GCGGGGAACA CATCGGTATT 
GACCAGGGCC AGATGACGTA TGATGGTCAG CACTGGCATG CCACAGAGGC TTGTTTCAGC 
TGTGCTCGCT GTAAGAAGTC TCTGCTCGGT CGTCCTTTTC TACCCAAGCA GGGTCAGATC 
TTCTGCTCTC GGGCTTGTAG TGTCGGCGAT GACCAAAACG GCTCTGATTC CTCAGATTCT 
GCATTTCAGA GCGCCCGTTC ACGTGAAGCC CGTCGCAGCA GCTCCAAATC CAACAAGAGC 
AGTACTGGAG GAAGTGGAGG AGATGGACAC CAGAGTGGGA AAGCTTCAGC TATGCGTTTC 
TCTGCTGATG TTGACCCACT GTCTCTGCAA ATGGATTTGC TCAGTCTGTC CAGTCAAACT 
CCTAGCTTAA ATCGAGAATC TCCATCCTGG AAGACGCAGG CTGAGATGTT TGGATATGAG 
GGTCGTAATG AACTCTCCAC AAATCCTCTT CATTTGCTCA GTCAGTGCAA CATTCGGACT 
TCCTATTCCT CAAATCTGTC CCCTGGTCAC CTATCAGATC CAAGGCCCAA GGAGCCTGCG 
TCTTCTAAGA GACCCCCTGT ATCTGCTCTA AAAGGGAAGT CTTTGAATGA GAACTGGTTC 
CAGCAGGGAC CAGAGGACTA TTATCCACCT GCACTGCGAA CCCAAGCGAG CTTTAACGAG 
GTTCCACACA ACAGCTTCAT GGATAAGCGC TCTGTAAGTC TGCATGTGTT CCAGAGGGAG 
AAGGAAAAGG ATGTTGGGCC ACAGATGAGC CGCAGCAGGA ACCCCATCAG TGCACTTGGC 
TTTAGTGAAC AGCTCACACC ACTGGAACAA ACACCACGCG GCTCTATGGA GTCACTGGCT 
CTATCTAATG CTACAGGCAC CTCTGCAGAT GGCGGCAGTA AAAGGCAGGA GCACTTGTCT 
CGCTTCTCAA TGCCGGACTT GAGTAAAGAT TCTGGCATGA ACGTGTCTGA AAAGAGCAAC 
ATGGGCACCC TCAACTCCTC TGTGCAGTTC CACAGCTCTG AATCTCTGCG CAGCCTGAAC 
TCGGCTCAGC CCTACCTGGA GCTCGAAGCT CCGGTCCAAG TCAGGTTTCC TGTGCAATAC 
CCCCGAGAAC CTTCTGGAAT TAATGCTTTG CCGCCTGGTT TTGCCTATCA GGAGGAAGAT 
CGCGTAAGTT TGGTTAGCAA CGCCAATAAT GCTCGTCTAC CACCAATGAG CGAACGCACC 
CGCCGTCGTA GTATAGATCA CGAAGAGCCA CGCAGACGAC ACCACCACCA TCGCTCCCGC 
CGCTCCAGGC GATCCCGTTC AGACAATGCA CTCAACTTAG TCGCTGAACG CCGCCCAGCT 
CCGAAACGAT CTCAACTTCA TGCTCGTGAG GATTACGACC AGTTCCCTCT TCCCCGTGGA 
TCTCGGGACA CTTACGGAAG TGGCAGACTG AGGCAACAGC CTTTCAGACC TTGCCCTCGA 
ACAACCTCTG ACCTCACGCT TCAAAACCCC GCTCTCCACC GACACCAGGG TCCTTATTCG 
TGGGATCAGT ATGATTACAA TGACGACTGG TGCTCCACAT GTTCCTCATC TTCCGAGTCA 
GAAGATGAAG GGTATTTTCT GGGTGAGCCA ATTCCCAGAC CCATCCAACT GAGGCACATG 
ACTAGCGAAG AGCTGCTACG CAAATACAAC TCTTCAGGAC TCGGAGCATC CGGTCAATTC 
GGCAGTCGAG GACAGTTACA TATGCGCAAA CGTAGGAAGA GCAAGAACTG CATTATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRICKLE2B ( XM_009305950.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304225.1 Danio rerio prickle homolog 2b (prickle2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa16980
Clone ID Related Accession (Same CDS sequence)	XM_005155827.3 , XM_009305950.2
Accession Version	XM_009305950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	prickle-like protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTGG AGATGGAGAA GACGGTGACC AAACTGATGT ACGACTTCCA GAGGAACTCT 
ACATCTGACG ATGACTCTGG CTGTGCTCTT GAGGAGTATG CTTGGGTGCC ACCCGGACTC 
AAACCTGAAC AGGTCCATCA GTATTACAGC TCTTTGCCGG AAGATAAAGT ACCATACGTG 
AACAGTCCTG GAGAGAAGTA TCGCATCAAA CAGCTCCTGC ATCAGCTTCC ACCCCATGAC 
AATGAGGTGA GATACTGCAA CAGTCTGGAT GATGAGGAGA AACGAGAGCT CAAGCTCTTC 
AGCAACCAGA GGAAGAGGGA GAACCTGGGC AGAGGAAACG TCAGACCCTT CCCCGTGACA 
ATGACCGGAG CCATCTGTGA ACAGTGTGGA GGACAGATCA ATGGAGGTGA CATTGCGGTC 
TTTGCATCAC GAGCAGGTCA TGGTGTTTGC TGGCACCCCC AGTGCTTTGT GTGCAGCATG 
TGTGATGAGC TGCTGGTGGA TCTGATCTAC TTCTACCAGG ACGGGAAGAT CTTCTGCGGT 
CGCCATCATG CAGAGAGACT CAAACCCCGC TGTTCAGCCT GTGATGAGAT TATTTTAGCA 
GACGAGTGCA CAGAGGCCGA GGGCAGGCAC TGGCACATGA AGCACTTCTG CTGTTTCGAG 
TGCGAGACAG TTCTGGGCGG CCAGCGCTAC ATCATGAAGG AGGGTCGGCC CTACTGTTGC 
ACCTGCTTCG AGTCCTTGTA TGCCGAATAC TGCGACTCCT GCGGGGAACA CATCGGTATT 
GACCAGGGCC AGATGACGTA TGATGGTCAG CACTGGCATG CCACAGAGGC TTGTTTCAGC 
TGTGCTCGCT GTAAGAAGTC TCTGCTCGGT CGTCCTTTTC TACCCAAGCA GGGTCAGATC 
TTCTGCTCTC GGGCTTGTAG TGTCGGCGAT GACCAAAACG GCTCTGATTC CTCAGATTCT 
GCATTTCAGA GCGCCCGTTC ACGTGAAGCC CGTCGCAGCA GCTCCAAATC CAACAAGAGC 
AGTACTGGAG GAAGTGGAGG AGATGGACAC CAGAGTGGGA AAGCTTCAGC TATGCGTTTC 
TCTGCTGATG TTGACCCACT GTCTCTGCAA ATGGATTTGC TCAGTCTGTC CAGTCAAACT 
CCTAGCTTAA ATCGAGAATC TCCATCCTGG AAGACGCAGG CTGAGATGTT TGGATATGAG 
GGTCGTAATG AACTCTCCAC AAATCCTCTT CATTTGCTCA GTCAGTGCAA CATTCGGACT 
TCCTATTCCT CAAATCTGTC CCCTGGTCAC CTATCAGATC CAAGGCCCAA GGAGCCTGCG 
TCTTCTAAGA GACCCCCTGT ATCTGCTCTA AAAGGGAAGT CTTTGAATGA GAACTGGTTC 
CAGCAGGGAC CAGAGGACTA TTATCCACCT GCACTGCGAA CCCAAGCGAG CTTTAACGAG 
GTTCCACACA ACAGCTTCAT GGATAAGCGC TCTGTAAGTC TGCATGTGTT CCAGAGGGAG 
AAGGAAAAGG ATGTTGGGCC ACAGATGAGC CGCAGCAGGA ACCCCATCAG TGCACTTGGC 
TTTAGTGAAC AGCTCACACC ACTGGAACAA ACACCACGCG GCTCTATGGA GTCACTGGCT 
CTATCTAATG CTACAGGCAC CTCTGCAGAT GGCGGCAGTA AAAGGCAGGA GCACTTGTCT 
CGCTTCTCAA TGCCGGACTT GAGTAAAGAT TCTGGCATGA ACGTGTCTGA AAAGAGCAAC 
ATGGGCACCC TCAACTCCTC TGTGCAGTTC CACAGCTCTG AATCTCTGCG CAGCCTGAAC 
TCGGCTCAGC CCTACCTGGA GCTCGAAGCT CCGGTCCAAG TCAGGTTTCC TGTGCAATAC 
CCCCGAGAAC CTTCTGGAAT TAATGCTTTG CCGCCTGGTT TTGCCTATCA GGAGGAAGAT 
CGCGTAAGTT TGGTTAGCAA CGCCAATAAT GCTCGTCTAC CACCAATGAG CGAACGCACC 
CGCCGTCGTA GTATAGATCA CGAAGAGCCA CGCAGACGAC ACCACCACCA TCGCTCCCGC 
CGCTCCAGGC GATCCCGTTC AGACAATGCA CTCAACTTAG TCGCTGAACG CCGCCCAGCT 
CCGAAACGAT CTCAACTTCA TGCTCGTGAG GATTACGACC AGTTCCCTCT TCCCCGTGGA 
TCTCGGGACA CTTACGGAAG TGGCAGACTG AGGCAACAGC CTTTCAGACC TTGCCCTCGA 
ACAACCTCTG ACCTCACGCT TCAAAACCCC GCTCTCCACC GACACCAGGG TCCTTATTCG 
TGGGATCAGT ATGATTACAA TGACGACTGG TGCTCCACAT GTTCCTCATC TTCCGAGTCA 
GAAGATGAAG GGTATTTTCT GGGTGAGCCA ATTCCCAGAC CCATCCAACT GAGGCACATG 
ACTAGCGAAG AGCTGCTACG CAAATACAAC TCTTCAGGAC TCGGAGCATC CGGTCAATTC 
GGCAGTCGAG GACAGTTACA TATGCGCAAA CGTAGGAAGA GCAAGAACTG CATTATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304225.1
CDS	441..2963
Translation	MPLEMEKTVTKLMYDFQRNSTSDDDSGCALEEYAWVPPGLKPEQVHQYYSSLPEDKVPYVNSPGEKYRIKQLLHQLPPHDNEVRYCNSLDDEEKRELKLFSNQRKRENLGRGNVRPFPVTMTGAICEQCGGQINGGDIAVFASRAGHGVCWHPQCFVCSMCDELLVDLIYFYQDGKIFCGRHHAERLKPRCSACDEIILADECTEAEGRHWHMKHFCCFECETVLGGQRYIMKEGRPYCCTCFESLYAEYCDSCGEHIGIDQGQMTYDGQHWHATEACFSCARCKKSLLGRPFLPKQGQIFCSRACSVGDDQNGSDSSDSAFQSARSREARRSSSKSNKSSTGGSGGDGHQSGKASAMRFSADVDPLSLQMDLLSLSSQTPSLNRESPSWKTQAEMFGYEGRNELSTNPLHLLSQCNIRTSYSSNLSPGHLSDPRPKEPASSKRPPVSALKGKSLNENWFQQGPEDYYPPALRTQASFNEVPHNSFMDKRSVSLHVFQREKEKDVGPQMSRSRNPISALGFSEQLTPLEQTPRGSMESLALSNATGTSADGGSKRQEHLSRFSMPDLSKDSGMNVSEKSNMGTLNSSVQFHSSESLRSLNSAQPYLELEAPVQVRFPVQYPREPSGINALPPGFAYQEEDRVSLVSNANNARLPPMSERTRRRSIDHEEPRRRHHHHRSRRSRRSRSDNALNLVAERRPAPKRSQLHAREDYDQFPLPRGSRDTYGSGRLRQQPFRPCPRTTSDLTLQNPALHRHQGPYSWDQYDYNDDWCSTCSSSSESEDEGYFLGEPIPRPIQLRHMTSEELLRKYNSSGLGASGQFGSRGQLHMRKRRKSKNCIIS

Target ORF information:

RefSeq Version	XM_009305950.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio prickle homolog 2b (prickle2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305950.2

ORF Insert Sequence:

ATGCCTCTGG AGATGGAGAA GACGGTGACC AAACTGATGT ACGACTTCCA GAGGAACTCT 
ACATCTGACG ATGACTCTGG CTGTGCTCTT GAGGAGTATG CTTGGGTGCC ACCCGGACTC 
AAACCTGAAC AGGTCCATCA GTATTACAGC TCTTTGCCGG AAGATAAAGT ACCATACGTG 
AACAGTCCTG GAGAGAAGTA TCGCATCAAA CAGCTCCTGC ATCAGCTTCC ACCCCATGAC 
AATGAGGTGA GATACTGCAA CAGTCTGGAT GATGAGGAGA AACGAGAGCT CAAGCTCTTC 
AGCAACCAGA GGAAGAGGGA GAACCTGGGC AGAGGAAACG TCAGACCCTT CCCCGTGACA 
ATGACCGGAG CCATCTGTGA ACAGTGTGGA GGACAGATCA ATGGAGGTGA CATTGCGGTC 
TTTGCATCAC GAGCAGGTCA TGGTGTTTGC TGGCACCCCC AGTGCTTTGT GTGCAGCATG 
TGTGATGAGC TGCTGGTGGA TCTGATCTAC TTCTACCAGG ACGGGAAGAT CTTCTGCGGT 
CGCCATCATG CAGAGAGACT CAAACCCCGC TGTTCAGCCT GTGATGAGAT TATTTTAGCA 
GACGAGTGCA CAGAGGCCGA GGGCAGGCAC TGGCACATGA AGCACTTCTG CTGTTTCGAG 
TGCGAGACAG TTCTGGGCGG CCAGCGCTAC ATCATGAAGG AGGGTCGGCC CTACTGTTGC 
ACCTGCTTCG AGTCCTTGTA TGCCGAATAC TGCGACTCCT GCGGGGAACA CATCGGTATT 
GACCAGGGCC AGATGACGTA TGATGGTCAG CACTGGCATG CCACAGAGGC TTGTTTCAGC 
TGTGCTCGCT GTAAGAAGTC TCTGCTCGGT CGTCCTTTTC TACCCAAGCA GGGTCAGATC 
TTCTGCTCTC GGGCTTGTAG TGTCGGCGAT GACCAAAACG GCTCTGATTC CTCAGATTCT 
GCATTTCAGA GCGCCCGTTC ACGTGAAGCC CGTCGCAGCA GCTCCAAATC CAACAAGAGC 
AGTACTGGAG GAAGTGGAGG AGATGGACAC CAGAGTGGGA AAGCTTCAGC TATGCGTTTC 
TCTGCTGATG TTGACCCACT GTCTCTGCAA ATGGATTTGC TCAGTCTGTC CAGTCAAACT 
CCTAGCTTAA ATCGAGAATC TCCATCCTGG AAGACGCAGG CTGAGATGTT TGGATATGAG 
GGTCGTAATG AACTCTCCAC AAATCCTCTT CATTTGCTCA GTCAGTGCAA CATTCGGACT 
TCCTATTCCT CAAATCTGTC CCCTGGTCAC CTATCAGATC CAAGGCCCAA GGAGCCTGCG 
TCTTCTAAGA GACCCCCTGT ATCTGCTCTA AAAGGGAAGT CTTTGAATGA GAACTGGTTC 
CAGCAGGGAC CAGAGGACTA TTATCCACCT GCACTGCGAA CCCAAGCGAG CTTTAACGAG 
GTTCCACACA ACAGCTTCAT GGATAAGCGC TCTGTAAGTC TGCATGTGTT CCAGAGGGAG 
AAGGAAAAGG ATGTTGGGCC ACAGATGAGC CGCAGCAGGA ACCCCATCAG TGCACTTGGC 
TTTAGTGAAC AGCTCACACC ACTGGAACAA ACACCACGCG GCTCTATGGA GTCACTGGCT 
CTATCTAATG CTACAGGCAC CTCTGCAGAT GGCGGCAGTA AAAGGCAGGA GCACTTGTCT 
CGCTTCTCAA TGCCGGACTT GAGTAAAGAT TCTGGCATGA ACGTGTCTGA AAAGAGCAAC 
ATGGGCACCC TCAACTCCTC TGTGCAGTTC CACAGCTCTG AATCTCTGCG CAGCCTGAAC 
TCGGCTCAGC CCTACCTGGA GCTCGAAGCT CCGGTCCAAG TCAGGTTTCC TGTGCAATAC 
CCCCGAGAAC CTTCTGGAAT TAATGCTTTG CCGCCTGGTT TTGCCTATCA GGAGGAAGAT 
CGCGTAAGTT TGGTTAGCAA CGCCAATAAT GCTCGTCTAC CACCAATGAG CGAACGCACC 
CGCCGTCGTA GTATAGATCA CGAAGAGCCA CGCAGACGAC ACCACCACCA TCGCTCCCGC 
CGCTCCAGGC GATCCCGTTC AGACAATGCA CTCAACTTAG TCGCTGAACG CCGCCCAGCT 
CCGAAACGAT CTCAACTTCA TGCTCGTGAG GATTACGACC AGTTCCCTCT TCCCCGTGGA 
TCTCGGGACA CTTACGGAAG TGGCAGACTG AGGCAACAGC CTTTCAGACC TTGCCCTCGA 
ACAACCTCTG ACCTCACGCT TCAAAACCCC GCTCTCCACC GACACCAGGG TCCTTATTCG 
TGGGATCAGT ATGATTACAA TGACGACTGG TGCTCCACAT GTTCCTCATC TTCCGAGTCA 
GAAGATGAAG GGTATTTTCT GGGTGAGCCA ATTCCCAGAC CCATCCAACT GAGGCACATG 
ACTAGCGAAG AGCTGCTACG CAAATACAAC TCTTCAGGAC TCGGAGCATC CGGTCAATTC 
GGCAGTCGAG GACAGTTACA TATGCGCAAA CGTAGGAAGA GCAAGAACTG CATTATATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional and evolutionary insights from the
Development (Cambridge, England)144(18)3375-3387(2017 09)
Reeves WM,Wu Y,Harder MJ,Veeman MT

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Functional characterization of Prickle2 and BBS7 identify overlapping phenotypes yet distinct mechanisms.
Developmental biology392(2)245-55(2014 Aug)
Mei X,Westfall TA,Zhang Q,Sheffield VC,Bassuk AG,Slusarski DC

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PRICKLE2B cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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