fn1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fn1b
Entrez Gene ID	334613
Full Name	fibronectin 1b
Synonyms	cb1057,fn1l,fn3,wu:fa14f11,wu:fb03c02
General protein information	Preferred Names fibronectin 1b Names fibronectin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160284.2	XP_005160341.1	fibronectin isoform X1
XM_009305554.2	XP_009303829.1	fibronectin isoform X2
XM_009305554.3	XP_009303829.1	fibronectin isoform X2
XM_005160284.3	XP_005160341.1	fibronectin isoform X1
NM_001013261.1	NP_001013279.1	fibronectin precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FN1	NP_001157250.1
Danio rerio (zebrafish)	fn1b	NP_001013279.1

Gene Ontology
Bibliography

Related articles in PubMed

Yap is required for scar formation but not myocyte proliferation during heart regeneration in zebrafish.
Flinn MA, Jeffery BE, O'Meara CC, Link BA
Cardiovascular research115(3)570-577(2019 03)

The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling.
Rasouli SJ, El-Brolosy M, Tsedeke AT, Bensimon-Brito A, Ghanbari P, Maischein HM, Kuenne C, Stainier DY
eLife7(2018 12)

Vangl2-dependent regulation of membrane protrusions and directed migration requires a fibronectin extracellular matrix.
Love AM, Prince DJ, Jessen JR
Development (Cambridge, England)145(22)(2018 11)

Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages in zebrafish spinal cord regeneration.
Tsarouchas TM, Wehner D, Cavone L, Munir T, Keatinge M, Lambertus M, Underhill A, Barrett T, Kassapis E, Ogryzko N, Feng Y, van Ham TJ, Becker T, Becker CG
Nature communications9(1)4670(2018 11)

Glypican 4 and Mmp14 interact in regulating the migration of anterior endodermal cells by limiting extracellular matrix deposition.
Hu B, Gao Y, Davies L, Woo S, Topczewski J, Jessen JR, Lin F
Development (Cambridge, England)145(17)(2018 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fn1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fn1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03905	NM_001013261.1 Latest version!	Danio rerio fibronectin 1b (fn1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa05388	XM_005160284.3 Latest version!	Danio rerio fibronectin 1b (fn1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa40007	XM_009305554.3 Latest version!	Danio rerio fibronectin 1b (fn1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa40007	XM_009305554.2	Danio rerio fibronectin 1b (fn1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa05388	XM_005160284.2	Danio rerio fibronectin 1b (fn1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fn1b ( NM_001013261.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001013279.1 Danio rerio fibronectin 1b (fn1b), mRNA.

Price & Availability↑

CloneID	ODa03905
Clone ID Related Accession (Same CDS sequence)	NM_001013261.1
Accession Version	NM_001013261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572624000000
Organism	Danio rerio(zebrafish)
Product	fibronectin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY538257.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY538257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG CAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGACA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTCGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGTGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC GCAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA TACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGGC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCTATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGGTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACCATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAAGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT ATCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCGGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGGG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGTATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCCGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CTGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGGC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCAA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGAGGAA ACTTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGA TGCTGTTCCA GCCCCCACAA ACCTGTACAT TTCTGAGGTG 
GGCGCAGACA GCATGCATGT GTCATGGACC GCACCTTCTG TTCAGCCATC TGAAATTAGT 
CGATTCGTCA TCCGCTACCA TCCAACAAAT AATGACGATG ACACTCAGGA AGTCAACGTA 
GGAGGTGGCA CCACTAGCTT CGTCCTTCAG AATTTGCTGC CAAATACAGA GTATCTGGTG 
AAAGTGGTGT GCGTCTATGA TGACAGAGAG AGTGAACCTG TAACTGGTAT CCAGAAAACA 
AAACTGGACT CCCCAACAAA TCTGGACTTC TCTGACGTCT CCACCAATTC ATTCACCGTG 
CACTGGATGG CCCCACGTGC AGTCATTACC GGTTACCGCC TACGTTATCA ACCAACAAGT 
GGTGGGCGTG CTAAAGATGA GAGACTTCCA CCAACAAGAA ACTACTTCAC GCTGGTGAAC 
CTGGCCCCTG AGACAGAGTA TACCGTTCAT ATTTATGCCG TCAGCAGCAA TATAGAAAGT 
CTACCTCTTA CTGGAACACA AGCAACTGTT TCTGATGCTC CCACTGACCT GGCTGTGACA 
TCCTCCACAC CCACCAGCAT TACCATCTCC TGGGATGCCC CGGCAGTTAC TGTACGCTAT 
TACAAGATCA CACATGGCGA AACTGGTGAA AGAGATGCAC CGAGAGAGTT CACTGTTCCA 
GGAACACAGT CCTCTGCCAC CATCCAGGGT CTGCGGCCTG ACACAGATTA CACCATTACG 
CTGTATGCTG TGACAGGAAG AGGGGACAGC CCAGCATCCA GCACACCAGT TATCATCACA 
CACAGGACTG CGGGTGGAAG CATTGCGTCG CCATCAGATT TAGATGTTAC AGATATTCAA 
GACAATACCT TGACTGTACG CTGGACCCCA GCTAGAGGTC CCATTACAGG GTACAGGGTA 
ACAGGGACAC CCAAGGGTGG GCAAGGTCCT ACATTCTCTG AACTAGTTGG CCCTGAGCGT 
ACAGAAATGA CCATCCATGG CTTGGTGCCC ACGGTGGAGT ATACAATCAA TGTGGTTGCT 
CTCAGCCAGA AAGGAGAAAG CACACCAGTG GTCCAAAAAG CTACAACCAC TTATGAACAG 
AAACCAAAAG ACCTGACCCT CACTGATGTG GACTCTAACT CCATGCTTGT GAAATGGAAT 
GGTCCACGGG TTCCAGGAGT GACCTCCTAC AGGGTTCTGT ATTCTAGTCC GGAGGAAGGC 
GAAAAAGAGT ACCAACCTGC CTTAAGTGGA CGTGACAACA GTGTGCTTCT TCAAGGTCTG 
AACCCCGGAA CCATGTACAT CATTAAAGTT ATTCCAATGA AAGGACGCAA CCCACTTCAA 
ACCCTTGAGG GCATACAGCA AACTATCCAA GAAGACACTC AGCGCATTGC TGTTCCTGCT 
CCCACCGATA TTCAGTTCTT CGACATCAGT CCGTCTTCCT TTATTGTGTC ATGGCAAGCA 
CCCAATTCCA GACTTTCTGG CTATCGTGTG GTGGTCACCC CAAGGAATCA GCTTGCCACA 
TCCAAAGAAA TGACCGTTTC ACCAGACTCT ACGCAAGTCT TAGTGCCTGG GCTTGTGGTG 
GCAACCCCTT ACAATGTACA TGTCTATGCA CTGAGGGGTT CAGACAGCAG TCCCCCTCTT 
ACTGGAGAAA TTACCACCGC CGACAATATT ACCCCTCCTC GCCGAGTACG TATCAGTGAT 
GTAAAAGACA CCTCATTCAC TCTCGCATGG CGTGCCGTCA ATAATGAGCC AATGACAGGA 
TTCCTTATTG AAGCCACGCC CAAAAATGGA GGTTACCCCA CCTTTAGACA AACTATTCCT 
TCTGACAGAC GTACCTATGT TGTCACTGGT TTGCATCCAG GTGTCATGTA TGTGGTCAAC 
ATGTACACAG TAAATGGAAA CATGAGGAGT CCTCCTTTCA CAATGACTGT AAACACAGCA 
CGTCCAGCTG TTCAGTCTCC AACCAACCTC CAATTCACCT CCCTAACCCC TAATTCCATC 
TCCTTCACTT GGCAAGCCCC TCCAACACAC ATCACAGGAT ATTATATTAC TTATGAAGAA 
TCAGGTGGCT CACCTCGTGA GCTTTTTCCA CGCAATAATG TTGGCCAGAA CTATGCCACT 
ATAGCCGGTC TAAAACCAGG CACTGAATAC ATTATTAAGA TCATTGCCCT TCTGAATAAC 
CAGAGAAGCG CACCACTAGT TGGCACAGCC ACAACTCAGC TGATATCCGG TCTACCTAGC 
CTGCCCAGTC ATGGTGGCCG AGACAAGCTG GATGTGCCAG AGCCTGACAA CAGAATTAAT 
ACAGTGGGTC CTACTGGTCA TGATTCAACG GATGCGCACG GGCAGCACGT GGAGTACACC 
GAATACAATA ATCAGCCCAA ACAACCCAAC ATAGGTTACC AGCCTCAAAA TCGTCAGCCA 
ATCTCTCAAC CTAACCAACC TCGACCACAG CCTTATGTGC CTCAAGTTGG AGAGACCCTA 
GTCTATGTCC CCCAGGTGGG ACCCGATGGG GGTCGTGTGC CTCAAATTAT TCAGGTTAGT 
GAGAGGCCTG GCGATGGCCA TACTTTTGGT TTTCCAGAGG ACAGGACAGG AAGGCCGCAG 
GAAGCTCAGA CCCAGACTAC CATCTCATGG CAGCCCGTCC AGCAGAGTCC CGCCTACCCA 
GCATCAGGTC AGCCAATCAC CCATCCAGAT GAGAAGATGT TCCAGATGCG TGTCCCAGGA 
ACATCCACAA CTGCCACTCT GATTGGTCTC ACCTCTGGTG CGTCCTATAA TGTCATTGTG 
GAGGCCTTGA AAGGAGCCCT CAAGCACAAA ATCCTAGAGG AAGTCGTCAC TGCTGGAAAT 
ACAGTTCCAG GAGATGCTAC ATCCAGCAAG GACTCGTGTT ATGACACTTT CACGGCCACT 
CACCACGATG TCGGAGCGGA ATGGGAGCGC ATGTCTGATA CAGGCTTTAA ACTCTGGTGT 
AGATGTCTTG GATTGGGAAG TGGACATTTC AGATGTGACT CTTCCAAATG GTGTCACGAC 
AGTGGAAATA ACTACCGCAT TGGCGAAAAG TGGGAGCGTC GGGCAGAAAA CGGTCACTTG 
ATGAGTTGCA CTTGCTTGGG CAATGGCAAA GGAGAATTCA AGTGTGAACC ACATGACTCC 
ATATGGTACG ATGATGGAAA GATGTACCAG ATGGGAAACC AGTGGCAGAA AGAATACTTG 
AGGGCCATCT GCACTTGCAC ATGCTATGGA GGACAACAGG GTTGGCGATG TGAGAACTGC 
AGAAGGCCAG GTGCTGAGAT AAATGCTGAC TTGCTTAGGC CTGTGCGCCT TAACAACGGC 
CAAAGGGTGA ATATTCAGTG CCCCATTGAA TGCCTNCGAC CAGAACTTCT TGCAGATGCT 
GTAGTCAATC CCAAAACCCA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013279.1
CDS	74..7300
Translation	MTRESVKRLLLLLCIGGSVHCMPQSAGKSKRQAQQQQIHLDTVYEEARSLAIHESGCNDNGRVYRMNDEWERPYMDSTLKCTCEGASGVKCRSKPAAEESCYDKFNARSYRVGETYERPKDNMIWDCTCIGSGKGKISCTIANRCHEGGNSYRIGDTWTRPHDTGDYMLECVCLGNGKGEWTCKPVAERCYDDSLGSSYVVGQTWQKPYQGWMIVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGETWSKIDSSGHTLQCLCTGNGRGEWKCDRHAASHVIPAIGTGSAVTRRVTPVMNQVNVLNELIEEGNCKTDSGVSYYNGMSWIRTQGTKEMLCTCVGGGISCEEQDGQSQVYGGNSGGQPCVFPFVFSGNTHYSCISEGRSDGQLWCSTTSDYDSDGLYSFCTQKNLLVATRGGNSNGALCQFPFKYNGRNYTDCTAEGRRDGMKWCGTTADYDREQKYGFCPMAAHEEVCTVNDVMYRVGDEWDKRHDTLGHMMRCTCQGNGRGEWNCISHTQLKDQCVVNGQTYDVNETFDKRHDQGYMMNCTCFGQGRGRWKCDAIDQCQEPETKVFYQIGQTWNKVIQGTPYRCSCYGNGIGEMACEPLQSTAPGRVIITEAGNQPNSHPIQWNAPPSAHITQYILKWRVKNTRTPWKEVTIPGHINSYTISGLKAGLTYEGQLISILRYGPREVTRFDFTTTYGSLAKAEGETTQPRQVVDTSESVTEITSNSFVISWVSASDTVSGFRVEYELSEEGARKRVLDLPRTTTSVNIQDLLPGRRYNVDVYEVNPEGDTKLILTTTQTTAPDAPTNHEVSNVQETSIVIRWSKPKAPITGYRVVYTPSVEGSSTELILPGTQTSVTLGDLRPGLSYNISIYSVEDNLESEPLVLQASTAGEQQPEEIQAPTDPQFYEVTDVKITITWTGPPNEVSGYCVTFAPVSTDGQAQRPLQLPVTHNAYAELMRLQPGTLYRFHIYAVSGGVESEPLIGEKSTKPDAPTDLRFTDITDDSALVIWSFPRAQVTGYRLFISIGSSSPKQLRIPGHKSQYKLPNLQPGTEYRVTLHSEQGNTLSEGITDTFRTTQPTGNAPRFNTEVTDTAIIVTWIPVAKFSYRMSVKPSQSGETPREETSGSGRIYISGLTPGLEYIYSLQPLFSGRKHGSPITNKVVTSLSPPTDLNVEPNSVNGELNVRWRGTKSPDITGYRVTGTPINGQRGVSLEESVRGEETSCILENLSPGVDYNISVYTVKNHLESEPISTSVTQDAVPAPTNLYISEVGADSMHVSWTAPSVQPSEISRFVIRYHPTNNDDDTQEVNVGGGTTSFVLQNLLPNTEYLVKVVCVYDDRESEPVTGIQKTKLDSPTNLDFSDVSTNSFTVHWMAPRAVITGYRLRYQPTSGGRAKDERLPPTRNYFTLVNLAPETEYTVHIYAVSSNIESLPLTGTQATVSDAPTDLAVTSSTPTSITISWDAPAVTVRYYKITHGETGERDAPREFTVPGTQSSATIQGLRPDTDYTITLYAVTGRGDSPASSTPVIITHRTAGGSIASPSDLDVTDIQDNTLTVRWTPARGPITGYRVTGTPKGGQGPTFSELVGPERTEMTIHGLVPTVEYTINVVALSQKGESTPVVQKATTTYEQKPKDLTLTDVDSNSMLVKWNGPRVPGVTSYRVLYSSPEEGEKEYQPALSGRDNSVLLQGLNPGTMYIIKVIPMKGRNPLQTLEGIQQTIQEDTQRIAVPAPTDIQFFDISPSSFIVSWQAPNSRLSGYRVVVTPRNQLATSKEMTVSPDSTQVLVPGLVVATPYNVHVYALRGSDSSPPLTGEITTADNITPPRRVRISDVKDTSFTLAWRAVNNEPMTGFLIEATPKNGGYPTFRQTIPSDRRTYVVTGLHPGVMYVVNMYTVNGNMRSPPFTMTVNTARPAVQSPTNLQFTSLTPNSISFTWQAPPTHITGYYITYEESGGSPRELFPRNNVGQNYATIAGLKPGTEYIIKIIALLNNQRSAPLVGTATTQLISGLPSLPSHGGRDKLDVPEPDNRINTVGPTGHDSTDAHGQHVEYTEYNNQPKQPNIGYQPQNRQPISQPNQPRPQPYVPQVGETLVYVPQVGPDGGRVPQIIQVSERPGDGHTFGFPEDRTGRPQEAQTQTTISWQPVQQSPAYPASGQPITHPDEKMFQMRVPGTSTTATLIGLTSGASYNVIVEALKGALKHKILEEVVTAGNTVPGDATSSKDSCYDTFTATHHDVGAEWERMSDTGFKLWCRCLGLGSGHFRCDSSKWCHDSGNNYRIGEKWERRAENGHLMSCTCLGNGKGEFKCEPHDSIWYDDGKMYQMGNQWQKEYLRAICTCTCYGGQQGWRCENCRRPGAEINADLLRPVRLNNGQRVNIQCPIECnullRPELLADAVVNPKTQE

Target ORF information:

RefSeq Version	NM_001013261.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fibronectin 1b (fn1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013261.1

ORF Insert Sequence:

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG CAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGACA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTCGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGTGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC GCAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA TACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGGC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCTATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGGTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACCATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAAGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT ATCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCGGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGGG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGTATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCCGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CTGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGGC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCAA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGAGGAA ACTTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGA TGCTGTTCCA GCCCCCACAA ACCTGTACAT TTCTGAGGTG 
GGCGCAGACA GCATGCATGT GTCATGGACC GCACCTTCTG TTCAGCCATC TGAAATTAGT 
CGATTCGTCA TCCGCTACCA TCCAACAAAT AATGACGATG ACACTCAGGA AGTCAACGTA 
GGAGGTGGCA CCACTAGCTT CGTCCTTCAG AATTTGCTGC CAAATACAGA GTATCTGGTG 
AAAGTGGTGT GCGTCTATGA TGACAGAGAG AGTGAACCTG TAACTGGTAT CCAGAAAACA 
AAACTGGACT CCCCAACAAA TCTGGACTTC TCTGACGTCT CCACCAATTC ATTCACCGTG 
CACTGGATGG CCCCACGTGC AGTCATTACC GGTTACCGCC TACGTTATCA ACCAACAAGT 
GGTGGGCGTG CTAAAGATGA GAGACTTCCA CCAACAAGAA ACTACTTCAC GCTGGTGAAC 
CTGGCCCCTG AGACAGAGTA TACCGTTCAT ATTTATGCCG TCAGCAGCAA TATAGAAAGT 
CTACCTCTTA CTGGAACACA AGCAACTGTT TCTGATGCTC CCACTGACCT GGCTGTGACA 
TCCTCCACAC CCACCAGCAT TACCATCTCC TGGGATGCCC CGGCAGTTAC TGTACGCTAT 
TACAAGATCA CACATGGCGA AACTGGTGAA AGAGATGCAC CGAGAGAGTT CACTGTTCCA 
GGAACACAGT CCTCTGCCAC CATCCAGGGT CTGCGGCCTG ACACAGATTA CACCATTACG 
CTGTATGCTG TGACAGGAAG AGGGGACAGC CCAGCATCCA GCACACCAGT TATCATCACA 
CACAGGACTG CGGGTGGAAG CATTGCGTCG CCATCAGATT TAGATGTTAC AGATATTCAA 
GACAATACCT TGACTGTACG CTGGACCCCA GCTAGAGGTC CCATTACAGG GTACAGGGTA 
ACAGGGACAC CCAAGGGTGG GCAAGGTCCT ACATTCTCTG AACTAGTTGG CCCTGAGCGT 
ACAGAAATGA CCATCCATGG CTTGGTGCCC ACGGTGGAGT ATACAATCAA TGTGGTTGCT 
CTCAGCCAGA AAGGAGAAAG CACACCAGTG GTCCAAAAAG CTACAACCAC TTATGAACAG 
AAACCAAAAG ACCTGACCCT CACTGATGTG GACTCTAACT CCATGCTTGT GAAATGGAAT 
GGTCCACGGG TTCCAGGAGT GACCTCCTAC AGGGTTCTGT ATTCTAGTCC GGAGGAAGGC 
GAAAAAGAGT ACCAACCTGC CTTAAGTGGA CGTGACAACA GTGTGCTTCT TCAAGGTCTG 
AACCCCGGAA CCATGTACAT CATTAAAGTT ATTCCAATGA AAGGACGCAA CCCACTTCAA 
ACCCTTGAGG GCATACAGCA AACTATCCAA GAAGACACTC AGCGCATTGC TGTTCCTGCT 
CCCACCGATA TTCAGTTCTT CGACATCAGT CCGTCTTCCT TTATTGTGTC ATGGCAAGCA 
CCCAATTCCA GACTTTCTGG CTATCGTGTG GTGGTCACCC CAAGGAATCA GCTTGCCACA 
TCCAAAGAAA TGACCGTTTC ACCAGACTCT ACGCAAGTCT TAGTGCCTGG GCTTGTGGTG 
GCAACCCCTT ACAATGTACA TGTCTATGCA CTGAGGGGTT CAGACAGCAG TCCCCCTCTT 
ACTGGAGAAA TTACCACCGC CGACAATATT ACCCCTCCTC GCCGAGTACG TATCAGTGAT 
GTAAAAGACA CCTCATTCAC TCTCGCATGG CGTGCCGTCA ATAATGAGCC AATGACAGGA 
TTCCTTATTG AAGCCACGCC CAAAAATGGA GGTTACCCCA CCTTTAGACA AACTATTCCT 
TCTGACAGAC GTACCTATGT TGTCACTGGT TTGCATCCAG GTGTCATGTA TGTGGTCAAC 
ATGTACACAG TAAATGGAAA CATGAGGAGT CCTCCTTTCA CAATGACTGT AAACACAGCA 
CGTCCAGCTG TTCAGTCTCC AACCAACCTC CAATTCACCT CCCTAACCCC TAATTCCATC 
TCCTTCACTT GGCAAGCCCC TCCAACACAC ATCACAGGAT ATTATATTAC TTATGAAGAA 
TCAGGTGGCT CACCTCGTGA GCTTTTTCCA CGCAATAATG TTGGCCAGAA CTATGCCACT 
ATAGCCGGTC TAAAACCAGG CACTGAATAC ATTATTAAGA TCATTGCCCT TCTGAATAAC 
CAGAGAAGCG CACCACTAGT TGGCACAGCC ACAACTCAGC TGATATCCGG TCTACCTAGC 
CTGCCCAGTC ATGGTGGCCG AGACAAGCTG GATGTGCCAG AGCCTGACAA CAGAATTAAT 
ACAGTGGGTC CTACTGGTCA TGATTCAACG GATGCGCACG GGCAGCACGT GGAGTACACC 
GAATACAATA ATCAGCCCAA ACAACCCAAC ATAGGTTACC AGCCTCAAAA TCGTCAGCCA 
ATCTCTCAAC CTAACCAACC TCGACCACAG CCTTATGTGC CTCAAGTTGG AGAGACCCTA 
GTCTATGTCC CCCAGGTGGG ACCCGATGGG GGTCGTGTGC CTCAAATTAT TCAGGTTAGT 
GAGAGGCCTG GCGATGGCCA TACTTTTGGT TTTCCAGAGG ACAGGACAGG AAGGCCGCAG 
GAAGCTCAGA CCCAGACTAC CATCTCATGG CAGCCCGTCC AGCAGAGTCC CGCCTACCCA 
GCATCAGGTC AGCCAATCAC CCATCCAGAT GAGAAGATGT TCCAGATGCG TGTCCCAGGA 
ACATCCACAA CTGCCACTCT GATTGGTCTC ACCTCTGGTG CGTCCTATAA TGTCATTGTG 
GAGGCCTTGA AAGGAGCCCT CAAGCACAAA ATCCTAGAGG AAGTCGTCAC TGCTGGAAAT 
ACAGTTCCAG GAGATGCTAC ATCCAGCAAG GACTCGTGTT ATGACACTTT CACGGCCACT 
CACCACGATG TCGGAGCGGA ATGGGAGCGC ATGTCTGATA CAGGCTTTAA ACTCTGGTGT 
AGATGTCTTG GATTGGGAAG TGGACATTTC AGATGTGACT CTTCCAAATG GTGTCACGAC 
AGTGGAAATA ACTACCGCAT TGGCGAAAAG TGGGAGCGTC GGGCAGAAAA CGGTCACTTG 
ATGAGTTGCA CTTGCTTGGG CAATGGCAAA GGAGAATTCA AGTGTGAACC ACATGACTCC 
ATATGGTACG ATGATGGAAA GATGTACCAG ATGGGAAACC AGTGGCAGAA AGAATACTTG 
AGGGCCATCT GCACTTGCAC ATGCTATGGA GGACAACAGG GTTGGCGATG TGAGAACTGC 
AGAAGGCCAG GTGCTGAGAT AAATGCTGAC TTGCTTAGGC CTGTGCGCCT TAACAACGGC 
CAAAGGGTGA ATATTCAGTG CCCCATTGAA TGCCTNCGAC CAGAACTTCT TGCAGATGCT 
GTAGTCAATC CCAAAACCCA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fn1b ( XM_005160284.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160341.1 Danio rerio fibronectin 1b (fn1b), transcript variant X1, mRNA.

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CloneID	ODa05388
Clone ID Related Accession (Same CDS sequence)	XM_005160284.2 , XM_005160284.3
Accession Version	XM_005160284.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	fibronectin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG TAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGATA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTTGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGCGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC ACAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA CACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGAC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCCATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGTTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACTATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAGGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT GTCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCAGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGAG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGCATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCTGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CCGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGAC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCGA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGATGAA ACCTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGC TGTTCCAGCC CCCACAAACC TGTACATTTC TGAGGTGGGC 
GCAGACAGCA TGCATGTGTC ATGGACCGCA CCTTCTGTTC AGCCATCTGA AATTAGTCGA 
TTTGTCATCC GCTACCATCC AACGAATAAT GACGATGACA CTCAGGAAGT CAACGTAGGA 
GGTGGCACCA CTAGCTTCGT CCTTCAGAAC TTGCTGCCAA ATACAGAGTA TCTGGTGAAA 
GTGGTGTGCG TCTATGATGA CAGAGAGAGT GAACCTGTAA CTGGTATCCA GAAAACAAAA 
CTGGACTCCC CAACAAATCT GGACTTCTCT GACGTCTCCA CCAATTCATT CACCGTGCAC 
TGGATGGCCC CACGTGCAGT CATTACCGGT TACCGCCTAC GTTATCAACC AACAAGTGGT 
GGGCGTGCTA AAGATGAGAG ACTTCCACCA ACAAGAAACT ACTTCACGCT GGTGAACCTG 
GCCCCTGAGA CAGAGTATAC CGTTCATATT TATGCCGTCA GCAGCAATAT AGAAAGTCTA 
CCACTTACTG GAACACAAGC AACTGTTTCT GATGCTCCCA CTGACCTGGC TGTGACATCC 
TCCACACCCA CCAGCATTAC CATCTCCTGG GATGCCCCGG CAGTTACCGT ACGCTATTAC 
AAGATCACAC ATGGCGAAAC TGGTGAAAGA GATGCACCGA GAGAGTTCAC TGTTCCAGGA 
ACACAGTCTT CTGCCACCAT CCAGGGTCTG CGGCCTGACA CAGATTACAC CATTACGCTG 
TATGCTGTGA CAGGAAGAGG GGACAGCCCA GCATCCAGCA CACCAGTTAT CATCACACAC 
AGGACTGCGG GTGGAAGCAT TGCGTCGCCA TCAGATTTAG ATGTTACAGA TATTCAAGAC 
AATACCTTGA CTGTACGCTG GACCCCAGCT AGAGGTCCCA TTACAGGGTA CAGGGTAACA 
GGGACACCCA AGGGTGGGCA AGGTCCTACA TTCTCTGAAC TAGTTGGCCC TGAGCGTACA 
GAAATGACCA TCCATGGCTT GGTGCCCACG GTGGAGTATA CAATCAATGT GGTTGCTCTC 
AGCCAAGAAG GAGAAAGCAC ACCAGTGGTC CAAAAAGCTA CAACAACTAA TGAACAGAAA 
CCAAAAGACC TGACCTTCAC TGATGTGGAC TCTACCTCCA TGCTTGTGAA ATGGAATGTT 
CCACGGGTTC CAGGAGTGAC CTCCTACAGG GTTCTGTATT CTAGTCCGGA GGAAGGCGAA 
AGAGAGTACC AACCTGCCTT AAGTGGACGT GATAACAGTG TGCTTCTTCA AGGTCTGAAC 
CCCGGAACCA TGTACATCAT TAAAGTTATT CCAATGAAAG GACGCAACCC ACTTCAAACC 
CTTGAGGGCA TACAGCAAAC TATCCAAGAA GACACTCAGC GCATTGCTGT TCCTGCTCCC 
ACCGATATTC AGTTCTTCGA CATCAGTCCG TCTTCCTTTA TTGTGTCATG GCAAGCACCC 
AATTCCAGAC TTTCTGGCTA TCGTGTGGTG GTCACCCCAA GGAATCAGCT TGCCACATCC 
AAAGAAATGA CCATTTCACC AGACTCTACG CAAGTCTTAG TGCCTGGGCT TGTGGTGGCA 
ACCCCTTACA ATGTACATGT CTATGCACTG AGGGGTTCAG ACAGCAGTCC CCCTCTTACT 
GGAGAAATTA CCACCGCCGA CAATATTACC CCTCCTCGCC GAGTACGTAT CAGTGATGTA 
AAAGACACCT CATTCACTCT CGCATGGCGT GCCGTCAATA ATGAGCCAAT GACAGGATTC 
CTTATTGAAG CCACGCCCAA AAATGGAGGT TACCCCACCT TTAGACAAAC TATTCCTTCT 
GACAGACGTA CCTATGTTGT CACTGGTTTG CATCCAGGTG TCATGTATGT GGTCAACATG 
TACACAGTAA ATGGAAACAT GAGAAGTCCT CCTTTCACAA TGACTGTAAA CACAGCACGT 
CCAGCTGTTC AGTCTCCAAC CAACCTCCAA TTCACCTCCC TAACCCCTAA TTCCATCTCC 
TTCACTTGGC AAGCCCCTCC AACACACATC ACAGGATATT ATATTACTTA TGAAGAATCA 
GGTGGCTCAC CTCGTGAGCT TTTTCCACGC AATAATGTTG GCCAGAACTA TGCCACTATA 
GCCGGTCTAA AACCAGGCAC TGAATACATT ATTAAGATCA TTGCCCTTCT GAATAACCAG 
AGAAGCGCAC CACTAGTTGG CACAGCCACA ACTCAGCTGA TATCCGGTCT ACCTAGCCTG 
CCCAGTCATG GTGGCCGAGA CAAGCTGGAT GTGCCAGAGC CTGACAACAG AATTAATACA 
GTGGGTCCTA CTGGTCATGA TTCAACGGAT GCGCACGGGC AGCACGTGGA GTACACCGAA 
TACAATAATC AGCCCAAACA ACCCAACATA GGTTACCAGC CTCAAAATCG TCAGCCAATC 
TCTCAACCTA ACCAACCTCG ACCACAGCCT TATGTGCCTC AAGTTGGAGA GACCCTAGTC 
TATGTCCCCC AGGTGGGACC CGATGGGGGT CGTGTGCCTC AAATTATTCA GGTTAGTGAG 
AGGCCTGGCG ATGGCCATAC TTTTGGTTTT CCAGAGGACA GGACAGGAAG GCCGCAGGAA 
GCTCAGACCC AGACTACCAT CTCATGGCAG CCCTTCCAGC AGAGTTCTGC CTACCTGGTA 
TCATGTCAGC CAATCACCCA TCCAGATGAG AAGATGTTCC AGATGCGTGT CCCAGGAACA 
TCCACAACTG CCACTCTTAT TGGTCTCACC TCTGGTGCGT CCTATAATGT CATTGTGGAG 
GCCTTGAAAG GAGCCCTCAA GCACAAAATC CTAGAGGAAG TCGTCACTGC TGGAAATACA 
GTTCCAGGAG ATGCTACATC CAGCAAGGAC TCGTGTTATG ACACTTTCAC GGCCACTCAC 
CACGATGTCG GAGCGGAATG GGAGCGCATG TCTGATACAG GCTTTAAACT CTGGTGTAGA 
TGTCTTGGAT TGGGAAGTGG ACATTTCAGA TGTGACTCTT CCAAATGGTG TCACGACAGC 
GGAAATAACT ACCGCATTGG CGAAAAGTGG GAGCGTCGGG CAGAAAACGG TCACTTGATG 
AGTTGCACTT GCTTGGGCAA TGGCAAAGGA GAGTTCAAGT GTGAACCACA TGACTCCATA 
TGTTACGATG ATGGAAAGAT GTACCAGATT GGAAACCAGT GGCAGAAAGA ATACTTGGGG 
GCCATCTGCA CTTGCACATG CTATGGAGGA CAACAGGGTT GGCGATGTGA GAACTGCAGA 
AGGCCAGGTG CTGAGATAAA TGCTGACTTG CTTAGGCCTG TGCGCCTTAA CAACGGCCAA 
AGGGTGAATA TTCAGTGCCC CATCGAATGC CTCCGACCAG AACTTCTTGC AGATGCTGTA 
GTCAATCCCA AAACCCAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160341.1
CDS	115..7338
Translation	MTRESVKRLLLLLCIGGSVHCMPQSAGKSKRQAQQQQIHLDTVYEEARSLAIHESGCNDNGRVYRMNDEWERPYMDSTLKCTCEGASGVKCRSKPAAEESCYDKFNARSYRVGETYERPKDNMIWDCTCIGSGKGKISCTIANRCHEGGNSYRIGDTWTRPHDTGDYMLECVCLGNGKGEWTCKPVAERCYDDSLGSSYVVGQTWQKPYQGWMIVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGETWSKIDSSGHTLQCLCTGNGRGEWKCDRHAASHVIPAIGTGSAVTHRVTPVMNQVNVLNELIEEGNCKTDSGVSYYNGMSWIRTQGTKEMLCTCVGGGISCEEQDGQSQVYGGNSGGQPCVFPFVFSGNTHYSCISEGRSDGQLWCSTTSDYDSDGLYSFCTQKNLLVTTRGGNSNGALCQFPFKYNGRNYTDCTAEGRRDGMKWCGTTADYDREQKYGFCPMAAHEEVCTVNDVMYRVGDEWDKRHDTLGHMMRCTCQGNGRGEWNCISHTQLKDQCVVNGQTYDVNETFDKRHDQGYMMNCTCFGQGRGRWKCDAIDQCQEPETKVFYQIGQTWNKVIQGTPYRCSCYGNGIGEMACEPLQSTAPVRVIITEAGNQPNSHPIQWNAPPSAHITQYILKWRVKNTRTPWKEVTIPGHINSYTISGLKAGLTYEGQLISILRYGPREVTRFDFTTTYGSLAKAEGETTQPRQVVDTSESVTEITSNSFVISWVSASDTVSGFRVEYELSEEGAQKRVLDLPRTTTSVNIQDLLPGRRYNVDVYEVNPEGDTKLILTTTQTTAPDAPTNHEVSNVQETSIVIRWSKPKAPITGYRVVYTPSVEGSSTELILPETQTSVTLGDLRPGLSYNISIYSVEDNLESEPLVLQASTAGEQQPEEIQAPTDLQFYEVTDVKITITWTGPPNEVSGYRVTFAPVSTDGQAQRPLQLPVTHNAYAELMRLQPGTLYRFHIYAVSGGVESEPLIGEKSTKPDAPTDLRFTDITDDSALVIWSFPRAQVTGYRLFISIGSSSPKQLRIPGHKSQYKLPNLQPDTEYRVTLHSEQGNTLSEGITDTFRTTQPTGNAPRFNTEVTDTAIIVTWIPVAKFSYRMSVKPSQSGETPREETSGSGRIYISGLTPGLEYIYSLQPLFSGRKHGSPITNKVVTSLSPPTDLNVEPNSVNGELNVRWRGTKSPDITGYRVTGTPINGQRGVSLEESVRGDETSCILENLSPGVDYNISVYTVKNHLESEPISTSVTQAVPAPTNLYISEVGADSMHVSWTAPSVQPSEISRFVIRYHPTNNDDDTQEVNVGGGTTSFVLQNLLPNTEYLVKVVCVYDDRESEPVTGIQKTKLDSPTNLDFSDVSTNSFTVHWMAPRAVITGYRLRYQPTSGGRAKDERLPPTRNYFTLVNLAPETEYTVHIYAVSSNIESLPLTGTQATVSDAPTDLAVTSSTPTSITISWDAPAVTVRYYKITHGETGERDAPREFTVPGTQSSATIQGLRPDTDYTITLYAVTGRGDSPASSTPVIITHRTAGGSIASPSDLDVTDIQDNTLTVRWTPARGPITGYRVTGTPKGGQGPTFSELVGPERTEMTIHGLVPTVEYTINVVALSQEGESTPVVQKATTTNEQKPKDLTFTDVDSTSMLVKWNVPRVPGVTSYRVLYSSPEEGEREYQPALSGRDNSVLLQGLNPGTMYIIKVIPMKGRNPLQTLEGIQQTIQEDTQRIAVPAPTDIQFFDISPSSFIVSWQAPNSRLSGYRVVVTPRNQLATSKEMTISPDSTQVLVPGLVVATPYNVHVYALRGSDSSPPLTGEITTADNITPPRRVRISDVKDTSFTLAWRAVNNEPMTGFLIEATPKNGGYPTFRQTIPSDRRTYVVTGLHPGVMYVVNMYTVNGNMRSPPFTMTVNTARPAVQSPTNLQFTSLTPNSISFTWQAPPTHITGYYITYEESGGSPRELFPRNNVGQNYATIAGLKPGTEYIIKIIALLNNQRSAPLVGTATTQLISGLPSLPSHGGRDKLDVPEPDNRINTVGPTGHDSTDAHGQHVEYTEYNNQPKQPNIGYQPQNRQPISQPNQPRPQPYVPQVGETLVYVPQVGPDGGRVPQIIQVSERPGDGHTFGFPEDRTGRPQEAQTQTTISWQPFQQSSAYLVSCQPITHPDEKMFQMRVPGTSTTATLIGLTSGASYNVIVEALKGALKHKILEEVVTAGNTVPGDATSSKDSCYDTFTATHHDVGAEWERMSDTGFKLWCRCLGLGSGHFRCDSSKWCHDSGNNYRIGEKWERRAENGHLMSCTCLGNGKGEFKCEPHDSICYDDGKMYQIGNQWQKEYLGAICTCTCYGGQQGWRCENCRRPGAEINADLLRPVRLNNGQRVNIQCPIECLRPELLADAVVNPKTQE

Target ORF information:

RefSeq Version	XM_005160284.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio fibronectin 1b (fn1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160284.2

ORF Insert Sequence:

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG TAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGATA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTTGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGCGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC ACAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA CACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGAC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCCATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGTTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACTATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAGGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT GTCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCAGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGAG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGCATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCTGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CCGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGAC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCGA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGATGAA ACCTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGC TGTTCCAGCC CCCACAAACC TGTACATTTC TGAGGTGGGC 
GCAGACAGCA TGCATGTGTC ATGGACCGCA CCTTCTGTTC AGCCATCTGA AATTAGTCGA 
TTTGTCATCC GCTACCATCC AACGAATAAT GACGATGACA CTCAGGAAGT CAACGTAGGA 
GGTGGCACCA CTAGCTTCGT CCTTCAGAAC TTGCTGCCAA ATACAGAGTA TCTGGTGAAA 
GTGGTGTGCG TCTATGATGA CAGAGAGAGT GAACCTGTAA CTGGTATCCA GAAAACAAAA 
CTGGACTCCC CAACAAATCT GGACTTCTCT GACGTCTCCA CCAATTCATT CACCGTGCAC 
TGGATGGCCC CACGTGCAGT CATTACCGGT TACCGCCTAC GTTATCAACC AACAAGTGGT 
GGGCGTGCTA AAGATGAGAG ACTTCCACCA ACAAGAAACT ACTTCACGCT GGTGAACCTG 
GCCCCTGAGA CAGAGTATAC CGTTCATATT TATGCCGTCA GCAGCAATAT AGAAAGTCTA 
CCACTTACTG GAACACAAGC AACTGTTTCT GATGCTCCCA CTGACCTGGC TGTGACATCC 
TCCACACCCA CCAGCATTAC CATCTCCTGG GATGCCCCGG CAGTTACCGT ACGCTATTAC 
AAGATCACAC ATGGCGAAAC TGGTGAAAGA GATGCACCGA GAGAGTTCAC TGTTCCAGGA 
ACACAGTCTT CTGCCACCAT CCAGGGTCTG CGGCCTGACA CAGATTACAC CATTACGCTG 
TATGCTGTGA CAGGAAGAGG GGACAGCCCA GCATCCAGCA CACCAGTTAT CATCACACAC 
AGGACTGCGG GTGGAAGCAT TGCGTCGCCA TCAGATTTAG ATGTTACAGA TATTCAAGAC 
AATACCTTGA CTGTACGCTG GACCCCAGCT AGAGGTCCCA TTACAGGGTA CAGGGTAACA 
GGGACACCCA AGGGTGGGCA AGGTCCTACA TTCTCTGAAC TAGTTGGCCC TGAGCGTACA 
GAAATGACCA TCCATGGCTT GGTGCCCACG GTGGAGTATA CAATCAATGT GGTTGCTCTC 
AGCCAAGAAG GAGAAAGCAC ACCAGTGGTC CAAAAAGCTA CAACAACTAA TGAACAGAAA 
CCAAAAGACC TGACCTTCAC TGATGTGGAC TCTACCTCCA TGCTTGTGAA ATGGAATGTT 
CCACGGGTTC CAGGAGTGAC CTCCTACAGG GTTCTGTATT CTAGTCCGGA GGAAGGCGAA 
AGAGAGTACC AACCTGCCTT AAGTGGACGT GATAACAGTG TGCTTCTTCA AGGTCTGAAC 
CCCGGAACCA TGTACATCAT TAAAGTTATT CCAATGAAAG GACGCAACCC ACTTCAAACC 
CTTGAGGGCA TACAGCAAAC TATCCAAGAA GACACTCAGC GCATTGCTGT TCCTGCTCCC 
ACCGATATTC AGTTCTTCGA CATCAGTCCG TCTTCCTTTA TTGTGTCATG GCAAGCACCC 
AATTCCAGAC TTTCTGGCTA TCGTGTGGTG GTCACCCCAA GGAATCAGCT TGCCACATCC 
AAAGAAATGA CCATTTCACC AGACTCTACG CAAGTCTTAG TGCCTGGGCT TGTGGTGGCA 
ACCCCTTACA ATGTACATGT CTATGCACTG AGGGGTTCAG ACAGCAGTCC CCCTCTTACT 
GGAGAAATTA CCACCGCCGA CAATATTACC CCTCCTCGCC GAGTACGTAT CAGTGATGTA 
AAAGACACCT CATTCACTCT CGCATGGCGT GCCGTCAATA ATGAGCCAAT GACAGGATTC 
CTTATTGAAG CCACGCCCAA AAATGGAGGT TACCCCACCT TTAGACAAAC TATTCCTTCT 
GACAGACGTA CCTATGTTGT CACTGGTTTG CATCCAGGTG TCATGTATGT GGTCAACATG 
TACACAGTAA ATGGAAACAT GAGAAGTCCT CCTTTCACAA TGACTGTAAA CACAGCACGT 
CCAGCTGTTC AGTCTCCAAC CAACCTCCAA TTCACCTCCC TAACCCCTAA TTCCATCTCC 
TTCACTTGGC AAGCCCCTCC AACACACATC ACAGGATATT ATATTACTTA TGAAGAATCA 
GGTGGCTCAC CTCGTGAGCT TTTTCCACGC AATAATGTTG GCCAGAACTA TGCCACTATA 
GCCGGTCTAA AACCAGGCAC TGAATACATT ATTAAGATCA TTGCCCTTCT GAATAACCAG 
AGAAGCGCAC CACTAGTTGG CACAGCCACA ACTCAGCTGA TATCCGGTCT ACCTAGCCTG 
CCCAGTCATG GTGGCCGAGA CAAGCTGGAT GTGCCAGAGC CTGACAACAG AATTAATACA 
GTGGGTCCTA CTGGTCATGA TTCAACGGAT GCGCACGGGC AGCACGTGGA GTACACCGAA 
TACAATAATC AGCCCAAACA ACCCAACATA GGTTACCAGC CTCAAAATCG TCAGCCAATC 
TCTCAACCTA ACCAACCTCG ACCACAGCCT TATGTGCCTC AAGTTGGAGA GACCCTAGTC 
TATGTCCCCC AGGTGGGACC CGATGGGGGT CGTGTGCCTC AAATTATTCA GGTTAGTGAG 
AGGCCTGGCG ATGGCCATAC TTTTGGTTTT CCAGAGGACA GGACAGGAAG GCCGCAGGAA 
GCTCAGACCC AGACTACCAT CTCATGGCAG CCCTTCCAGC AGAGTTCTGC CTACCTGGTA 
TCATGTCAGC CAATCACCCA TCCAGATGAG AAGATGTTCC AGATGCGTGT CCCAGGAACA 
TCCACAACTG CCACTCTTAT TGGTCTCACC TCTGGTGCGT CCTATAATGT CATTGTGGAG 
GCCTTGAAAG GAGCCCTCAA GCACAAAATC CTAGAGGAAG TCGTCACTGC TGGAAATACA 
GTTCCAGGAG ATGCTACATC CAGCAAGGAC TCGTGTTATG ACACTTTCAC GGCCACTCAC 
CACGATGTCG GAGCGGAATG GGAGCGCATG TCTGATACAG GCTTTAAACT CTGGTGTAGA 
TGTCTTGGAT TGGGAAGTGG ACATTTCAGA TGTGACTCTT CCAAATGGTG TCACGACAGC 
GGAAATAACT ACCGCATTGG CGAAAAGTGG GAGCGTCGGG CAGAAAACGG TCACTTGATG 
AGTTGCACTT GCTTGGGCAA TGGCAAAGGA GAGTTCAAGT GTGAACCACA TGACTCCATA 
TGTTACGATG ATGGAAAGAT GTACCAGATT GGAAACCAGT GGCAGAAAGA ATACTTGGGG 
GCCATCTGCA CTTGCACATG CTATGGAGGA CAACAGGGTT GGCGATGTGA GAACTGCAGA 
AGGCCAGGTG CTGAGATAAA TGCTGACTTG CTTAGGCCTG TGCGCCTTAA CAACGGCCAA 
AGGGTGAATA TTCAGTGCCC CATCGAATGC CTCCGACCAG AACTTCTTGC AGATGCTGTA 
GTCAATCCCA AAACCCAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fn1b ( XM_005160284.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160341.1 Danio rerio fibronectin 1b (fn1b), transcript variant X1, mRNA.

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CloneID	ODa05388
Clone ID Related Accession (Same CDS sequence)	XM_005160284.2 , XM_005160284.3
Accession Version	XM_005160284.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	fibronectin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160284.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG TAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGATA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTTGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGCGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC ACAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA CACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGAC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCCATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGTTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACTATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAGGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT GTCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCAGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGAG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGCATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCTGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CCGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGAC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCGA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGATGAA ACCTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGC TGTTCCAGCC CCCACAAACC TGTACATTTC TGAGGTGGGC 
GCAGACAGCA TGCATGTGTC ATGGACCGCA CCTTCTGTTC AGCCATCTGA AATTAGTCGA 
TTTGTCATCC GCTACCATCC AACGAATAAT GACGATGACA CTCAGGAAGT CAACGTAGGA 
GGTGGCACCA CTAGCTTCGT CCTTCAGAAC TTGCTGCCAA ATACAGAGTA TCTGGTGAAA 
GTGGTGTGCG TCTATGATGA CAGAGAGAGT GAACCTGTAA CTGGTATCCA GAAAACAAAA 
CTGGACTCCC CAACAAATCT GGACTTCTCT GACGTCTCCA CCAATTCATT CACCGTGCAC 
TGGATGGCCC CACGTGCAGT CATTACCGGT TACCGCCTAC GTTATCAACC AACAAGTGGT 
GGGCGTGCTA AAGATGAGAG ACTTCCACCA ACAAGAAACT ACTTCACGCT GGTGAACCTG 
GCCCCTGAGA CAGAGTATAC CGTTCATATT TATGCCGTCA GCAGCAATAT AGAAAGTCTA 
CCACTTACTG GAACACAAGC AACTGTTTCT GATGCTCCCA CTGACCTGGC TGTGACATCC 
TCCACACCCA CCAGCATTAC CATCTCCTGG GATGCCCCGG CAGTTACCGT ACGCTATTAC 
AAGATCACAC ATGGCGAAAC TGGTGAAAGA GATGCACCGA GAGAGTTCAC TGTTCCAGGA 
ACACAGTCTT CTGCCACCAT CCAGGGTCTG CGGCCTGACA CAGATTACAC CATTACGCTG 
TATGCTGTGA CAGGAAGAGG GGACAGCCCA GCATCCAGCA CACCAGTTAT CATCACACAC 
AGGACTGCGG GTGGAAGCAT TGCGTCGCCA TCAGATTTAG ATGTTACAGA TATTCAAGAC 
AATACCTTGA CTGTACGCTG GACCCCAGCT AGAGGTCCCA TTACAGGGTA CAGGGTAACA 
GGGACACCCA AGGGTGGGCA AGGTCCTACA TTCTCTGAAC TAGTTGGCCC TGAGCGTACA 
GAAATGACCA TCCATGGCTT GGTGCCCACG GTGGAGTATA CAATCAATGT GGTTGCTCTC 
AGCCAAGAAG GAGAAAGCAC ACCAGTGGTC CAAAAAGCTA CAACAACTAA TGAACAGAAA 
CCAAAAGACC TGACCTTCAC TGATGTGGAC TCTACCTCCA TGCTTGTGAA ATGGAATGTT 
CCACGGGTTC CAGGAGTGAC CTCCTACAGG GTTCTGTATT CTAGTCCGGA GGAAGGCGAA 
AGAGAGTACC AACCTGCCTT AAGTGGACGT GATAACAGTG TGCTTCTTCA AGGTCTGAAC 
CCCGGAACCA TGTACATCAT TAAAGTTATT CCAATGAAAG GACGCAACCC ACTTCAAACC 
CTTGAGGGCA TACAGCAAAC TATCCAAGAA GACACTCAGC GCATTGCTGT TCCTGCTCCC 
ACCGATATTC AGTTCTTCGA CATCAGTCCG TCTTCCTTTA TTGTGTCATG GCAAGCACCC 
AATTCCAGAC TTTCTGGCTA TCGTGTGGTG GTCACCCCAA GGAATCAGCT TGCCACATCC 
AAAGAAATGA CCATTTCACC AGACTCTACG CAAGTCTTAG TGCCTGGGCT TGTGGTGGCA 
ACCCCTTACA ATGTACATGT CTATGCACTG AGGGGTTCAG ACAGCAGTCC CCCTCTTACT 
GGAGAAATTA CCACCGCCGA CAATATTACC CCTCCTCGCC GAGTACGTAT CAGTGATGTA 
AAAGACACCT CATTCACTCT CGCATGGCGT GCCGTCAATA ATGAGCCAAT GACAGGATTC 
CTTATTGAAG CCACGCCCAA AAATGGAGGT TACCCCACCT TTAGACAAAC TATTCCTTCT 
GACAGACGTA CCTATGTTGT CACTGGTTTG CATCCAGGTG TCATGTATGT GGTCAACATG 
TACACAGTAA ATGGAAACAT GAGAAGTCCT CCTTTCACAA TGACTGTAAA CACAGCACGT 
CCAGCTGTTC AGTCTCCAAC CAACCTCCAA TTCACCTCCC TAACCCCTAA TTCCATCTCC 
TTCACTTGGC AAGCCCCTCC AACACACATC ACAGGATATT ATATTACTTA TGAAGAATCA 
GGTGGCTCAC CTCGTGAGCT TTTTCCACGC AATAATGTTG GCCAGAACTA TGCCACTATA 
GCCGGTCTAA AACCAGGCAC TGAATACATT ATTAAGATCA TTGCCCTTCT GAATAACCAG 
AGAAGCGCAC CACTAGTTGG CACAGCCACA ACTCAGCTGA TATCCGGTCT ACCTAGCCTG 
CCCAGTCATG GTGGCCGAGA CAAGCTGGAT GTGCCAGAGC CTGACAACAG AATTAATACA 
GTGGGTCCTA CTGGTCATGA TTCAACGGAT GCGCACGGGC AGCACGTGGA GTACACCGAA 
TACAATAATC AGCCCAAACA ACCCAACATA GGTTACCAGC CTCAAAATCG TCAGCCAATC 
TCTCAACCTA ACCAACCTCG ACCACAGCCT TATGTGCCTC AAGTTGGAGA GACCCTAGTC 
TATGTCCCCC AGGTGGGACC CGATGGGGGT CGTGTGCCTC AAATTATTCA GGTTAGTGAG 
AGGCCTGGCG ATGGCCATAC TTTTGGTTTT CCAGAGGACA GGACAGGAAG GCCGCAGGAA 
GCTCAGACCC AGACTACCAT CTCATGGCAG CCCTTCCAGC AGAGTTCTGC CTACCTGGTA 
TCATGTCAGC CAATCACCCA TCCAGATGAG AAGATGTTCC AGATGCGTGT CCCAGGAACA 
TCCACAACTG CCACTCTTAT TGGTCTCACC TCTGGTGCGT CCTATAATGT CATTGTGGAG 
GCCTTGAAAG GAGCCCTCAA GCACAAAATC CTAGAGGAAG TCGTCACTGC TGGAAATACA 
GTTCCAGGAG ATGCTACATC CAGCAAGGAC TCGTGTTATG ACACTTTCAC GGCCACTCAC 
CACGATGTCG GAGCGGAATG GGAGCGCATG TCTGATACAG GCTTTAAACT CTGGTGTAGA 
TGTCTTGGAT TGGGAAGTGG ACATTTCAGA TGTGACTCTT CCAAATGGTG TCACGACAGC 
GGAAATAACT ACCGCATTGG CGAAAAGTGG GAGCGTCGGG CAGAAAACGG TCACTTGATG 
AGTTGCACTT GCTTGGGCAA TGGCAAAGGA GAGTTCAAGT GTGAACCACA TGACTCCATA 
TGTTACGATG ATGGAAAGAT GTACCAGATT GGAAACCAGT GGCAGAAAGA ATACTTGGGG 
GCCATCTGCA CTTGCACATG CTATGGAGGA CAACAGGGTT GGCGATGTGA GAACTGCAGA 
AGGCCAGGTG CTGAGATAAA TGCTGACTTG CTTAGGCCTG TGCGCCTTAA CAACGGCCAA 
AGGGTGAATA TTCAGTGCCC CATCGAATGC CTCCGACCAG AACTTCTTGC AGATGCTGTA 
GTCAATCCCA AAACCCAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160341.1
CDS	109..7332
Translation	MTRESVKRLLLLLCIGGSVHCMPQSAGKSKRQAQQQQIHLDTVYEEARSLAIHESGCNDNGRVYRMNDEWERPYMDSTLKCTCEGASGVKCRSKPAAEESCYDKFNARSYRVGETYERPKDNMIWDCTCIGSGKGKISCTIANRCHEGGNSYRIGDTWTRPHDTGDYMLECVCLGNGKGEWTCKPVAERCYDDSLGSSYVVGQTWQKPYQGWMIVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGETWSKIDSSGHTLQCLCTGNGRGEWKCDRHAASHVIPAIGTGSAVTHRVTPVMNQVNVLNELIEEGNCKTDSGVSYYNGMSWIRTQGTKEMLCTCVGGGISCEEQDGQSQVYGGNSGGQPCVFPFVFSGNTHYSCISEGRSDGQLWCSTTSDYDSDGLYSFCTQKNLLVTTRGGNSNGALCQFPFKYNGRNYTDCTAEGRRDGMKWCGTTADYDREQKYGFCPMAAHEEVCTVNDVMYRVGDEWDKRHDTLGHMMRCTCQGNGRGEWNCISHTQLKDQCVVNGQTYDVNETFDKRHDQGYMMNCTCFGQGRGRWKCDAIDQCQEPETKVFYQIGQTWNKVIQGTPYRCSCYGNGIGEMACEPLQSTAPVRVIITEAGNQPNSHPIQWNAPPSAHITQYILKWRVKNTRTPWKEVTIPGHINSYTISGLKAGLTYEGQLISILRYGPREVTRFDFTTTYGSLAKAEGETTQPRQVVDTSESVTEITSNSFVISWVSASDTVSGFRVEYELSEEGAQKRVLDLPRTTTSVNIQDLLPGRRYNVDVYEVNPEGDTKLILTTTQTTAPDAPTNHEVSNVQETSIVIRWSKPKAPITGYRVVYTPSVEGSSTELILPETQTSVTLGDLRPGLSYNISIYSVEDNLESEPLVLQASTAGEQQPEEIQAPTDLQFYEVTDVKITITWTGPPNEVSGYRVTFAPVSTDGQAQRPLQLPVTHNAYAELMRLQPGTLYRFHIYAVSGGVESEPLIGEKSTKPDAPTDLRFTDITDDSALVIWSFPRAQVTGYRLFISIGSSSPKQLRIPGHKSQYKLPNLQPDTEYRVTLHSEQGNTLSEGITDTFRTTQPTGNAPRFNTEVTDTAIIVTWIPVAKFSYRMSVKPSQSGETPREETSGSGRIYISGLTPGLEYIYSLQPLFSGRKHGSPITNKVVTSLSPPTDLNVEPNSVNGELNVRWRGTKSPDITGYRVTGTPINGQRGVSLEESVRGDETSCILENLSPGVDYNISVYTVKNHLESEPISTSVTQAVPAPTNLYISEVGADSMHVSWTAPSVQPSEISRFVIRYHPTNNDDDTQEVNVGGGTTSFVLQNLLPNTEYLVKVVCVYDDRESEPVTGIQKTKLDSPTNLDFSDVSTNSFTVHWMAPRAVITGYRLRYQPTSGGRAKDERLPPTRNYFTLVNLAPETEYTVHIYAVSSNIESLPLTGTQATVSDAPTDLAVTSSTPTSITISWDAPAVTVRYYKITHGETGERDAPREFTVPGTQSSATIQGLRPDTDYTITLYAVTGRGDSPASSTPVIITHRTAGGSIASPSDLDVTDIQDNTLTVRWTPARGPITGYRVTGTPKGGQGPTFSELVGPERTEMTIHGLVPTVEYTINVVALSQEGESTPVVQKATTTNEQKPKDLTFTDVDSTSMLVKWNVPRVPGVTSYRVLYSSPEEGEREYQPALSGRDNSVLLQGLNPGTMYIIKVIPMKGRNPLQTLEGIQQTIQEDTQRIAVPAPTDIQFFDISPSSFIVSWQAPNSRLSGYRVVVTPRNQLATSKEMTISPDSTQVLVPGLVVATPYNVHVYALRGSDSSPPLTGEITTADNITPPRRVRISDVKDTSFTLAWRAVNNEPMTGFLIEATPKNGGYPTFRQTIPSDRRTYVVTGLHPGVMYVVNMYTVNGNMRSPPFTMTVNTARPAVQSPTNLQFTSLTPNSISFTWQAPPTHITGYYITYEESGGSPRELFPRNNVGQNYATIAGLKPGTEYIIKIIALLNNQRSAPLVGTATTQLISGLPSLPSHGGRDKLDVPEPDNRINTVGPTGHDSTDAHGQHVEYTEYNNQPKQPNIGYQPQNRQPISQPNQPRPQPYVPQVGETLVYVPQVGPDGGRVPQIIQVSERPGDGHTFGFPEDRTGRPQEAQTQTTISWQPFQQSSAYLVSCQPITHPDEKMFQMRVPGTSTTATLIGLTSGASYNVIVEALKGALKHKILEEVVTAGNTVPGDATSSKDSCYDTFTATHHDVGAEWERMSDTGFKLWCRCLGLGSGHFRCDSSKWCHDSGNNYRIGEKWERRAENGHLMSCTCLGNGKGEFKCEPHDSICYDDGKMYQIGNQWQKEYLGAICTCTCYGGQQGWRCENCRRPGAEINADLLRPVRLNNGQRVNIQCPIECLRPELLADAVVNPKTQE

Target ORF information:

RefSeq Version	XM_005160284.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fibronectin 1b (fn1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160284.3

ORF Insert Sequence:

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG TAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGATA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTTGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGCGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC ACAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA CACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGAC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCCATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGTTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACTATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAGGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT GTCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCAGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGAG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGCATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCTGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CCGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGAC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCGA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGATGAA ACCTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGC TGTTCCAGCC CCCACAAACC TGTACATTTC TGAGGTGGGC 
GCAGACAGCA TGCATGTGTC ATGGACCGCA CCTTCTGTTC AGCCATCTGA AATTAGTCGA 
TTTGTCATCC GCTACCATCC AACGAATAAT GACGATGACA CTCAGGAAGT CAACGTAGGA 
GGTGGCACCA CTAGCTTCGT CCTTCAGAAC TTGCTGCCAA ATACAGAGTA TCTGGTGAAA 
GTGGTGTGCG TCTATGATGA CAGAGAGAGT GAACCTGTAA CTGGTATCCA GAAAACAAAA 
CTGGACTCCC CAACAAATCT GGACTTCTCT GACGTCTCCA CCAATTCATT CACCGTGCAC 
TGGATGGCCC CACGTGCAGT CATTACCGGT TACCGCCTAC GTTATCAACC AACAAGTGGT 
GGGCGTGCTA AAGATGAGAG ACTTCCACCA ACAAGAAACT ACTTCACGCT GGTGAACCTG 
GCCCCTGAGA CAGAGTATAC CGTTCATATT TATGCCGTCA GCAGCAATAT AGAAAGTCTA 
CCACTTACTG GAACACAAGC AACTGTTTCT GATGCTCCCA CTGACCTGGC TGTGACATCC 
TCCACACCCA CCAGCATTAC CATCTCCTGG GATGCCCCGG CAGTTACCGT ACGCTATTAC 
AAGATCACAC ATGGCGAAAC TGGTGAAAGA GATGCACCGA GAGAGTTCAC TGTTCCAGGA 
ACACAGTCTT CTGCCACCAT CCAGGGTCTG CGGCCTGACA CAGATTACAC CATTACGCTG 
TATGCTGTGA CAGGAAGAGG GGACAGCCCA GCATCCAGCA CACCAGTTAT CATCACACAC 
AGGACTGCGG GTGGAAGCAT TGCGTCGCCA TCAGATTTAG ATGTTACAGA TATTCAAGAC 
AATACCTTGA CTGTACGCTG GACCCCAGCT AGAGGTCCCA TTACAGGGTA CAGGGTAACA 
GGGACACCCA AGGGTGGGCA AGGTCCTACA TTCTCTGAAC TAGTTGGCCC TGAGCGTACA 
GAAATGACCA TCCATGGCTT GGTGCCCACG GTGGAGTATA CAATCAATGT GGTTGCTCTC 
AGCCAAGAAG GAGAAAGCAC ACCAGTGGTC CAAAAAGCTA CAACAACTAA TGAACAGAAA 
CCAAAAGACC TGACCTTCAC TGATGTGGAC TCTACCTCCA TGCTTGTGAA ATGGAATGTT 
CCACGGGTTC CAGGAGTGAC CTCCTACAGG GTTCTGTATT CTAGTCCGGA GGAAGGCGAA 
AGAGAGTACC AACCTGCCTT AAGTGGACGT GATAACAGTG TGCTTCTTCA AGGTCTGAAC 
CCCGGAACCA TGTACATCAT TAAAGTTATT CCAATGAAAG GACGCAACCC ACTTCAAACC 
CTTGAGGGCA TACAGCAAAC TATCCAAGAA GACACTCAGC GCATTGCTGT TCCTGCTCCC 
ACCGATATTC AGTTCTTCGA CATCAGTCCG TCTTCCTTTA TTGTGTCATG GCAAGCACCC 
AATTCCAGAC TTTCTGGCTA TCGTGTGGTG GTCACCCCAA GGAATCAGCT TGCCACATCC 
AAAGAAATGA CCATTTCACC AGACTCTACG CAAGTCTTAG TGCCTGGGCT TGTGGTGGCA 
ACCCCTTACA ATGTACATGT CTATGCACTG AGGGGTTCAG ACAGCAGTCC CCCTCTTACT 
GGAGAAATTA CCACCGCCGA CAATATTACC CCTCCTCGCC GAGTACGTAT CAGTGATGTA 
AAAGACACCT CATTCACTCT CGCATGGCGT GCCGTCAATA ATGAGCCAAT GACAGGATTC 
CTTATTGAAG CCACGCCCAA AAATGGAGGT TACCCCACCT TTAGACAAAC TATTCCTTCT 
GACAGACGTA CCTATGTTGT CACTGGTTTG CATCCAGGTG TCATGTATGT GGTCAACATG 
TACACAGTAA ATGGAAACAT GAGAAGTCCT CCTTTCACAA TGACTGTAAA CACAGCACGT 
CCAGCTGTTC AGTCTCCAAC CAACCTCCAA TTCACCTCCC TAACCCCTAA TTCCATCTCC 
TTCACTTGGC AAGCCCCTCC AACACACATC ACAGGATATT ATATTACTTA TGAAGAATCA 
GGTGGCTCAC CTCGTGAGCT TTTTCCACGC AATAATGTTG GCCAGAACTA TGCCACTATA 
GCCGGTCTAA AACCAGGCAC TGAATACATT ATTAAGATCA TTGCCCTTCT GAATAACCAG 
AGAAGCGCAC CACTAGTTGG CACAGCCACA ACTCAGCTGA TATCCGGTCT ACCTAGCCTG 
CCCAGTCATG GTGGCCGAGA CAAGCTGGAT GTGCCAGAGC CTGACAACAG AATTAATACA 
GTGGGTCCTA CTGGTCATGA TTCAACGGAT GCGCACGGGC AGCACGTGGA GTACACCGAA 
TACAATAATC AGCCCAAACA ACCCAACATA GGTTACCAGC CTCAAAATCG TCAGCCAATC 
TCTCAACCTA ACCAACCTCG ACCACAGCCT TATGTGCCTC AAGTTGGAGA GACCCTAGTC 
TATGTCCCCC AGGTGGGACC CGATGGGGGT CGTGTGCCTC AAATTATTCA GGTTAGTGAG 
AGGCCTGGCG ATGGCCATAC TTTTGGTTTT CCAGAGGACA GGACAGGAAG GCCGCAGGAA 
GCTCAGACCC AGACTACCAT CTCATGGCAG CCCTTCCAGC AGAGTTCTGC CTACCTGGTA 
TCATGTCAGC CAATCACCCA TCCAGATGAG AAGATGTTCC AGATGCGTGT CCCAGGAACA 
TCCACAACTG CCACTCTTAT TGGTCTCACC TCTGGTGCGT CCTATAATGT CATTGTGGAG 
GCCTTGAAAG GAGCCCTCAA GCACAAAATC CTAGAGGAAG TCGTCACTGC TGGAAATACA 
GTTCCAGGAG ATGCTACATC CAGCAAGGAC TCGTGTTATG ACACTTTCAC GGCCACTCAC 
CACGATGTCG GAGCGGAATG GGAGCGCATG TCTGATACAG GCTTTAAACT CTGGTGTAGA 
TGTCTTGGAT TGGGAAGTGG ACATTTCAGA TGTGACTCTT CCAAATGGTG TCACGACAGC 
GGAAATAACT ACCGCATTGG CGAAAAGTGG GAGCGTCGGG CAGAAAACGG TCACTTGATG 
AGTTGCACTT GCTTGGGCAA TGGCAAAGGA GAGTTCAAGT GTGAACCACA TGACTCCATA 
TGTTACGATG ATGGAAAGAT GTACCAGATT GGAAACCAGT GGCAGAAAGA ATACTTGGGG 
GCCATCTGCA CTTGCACATG CTATGGAGGA CAACAGGGTT GGCGATGTGA GAACTGCAGA 
AGGCCAGGTG CTGAGATAAA TGCTGACTTG CTTAGGCCTG TGCGCCTTAA CAACGGCCAA 
AGGGTGAATA TTCAGTGCCC CATCGAATGC CTCCGACCAG AACTTCTTGC AGATGCTGTA 
GTCAATCCCA AAACCCAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fn1b ( XM_009305554.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303829.1 Danio rerio fibronectin 1b (fn1b), transcript variant X2, mRNA.

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CloneID	ODa40007
Clone ID Related Accession (Same CDS sequence)	XM_009305554.2 , XM_009305554.3
Accession Version	XM_009305554.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	fibronectin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305554.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG TAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGATA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTTGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGCGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC ACAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA CACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGAC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCCATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGTTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACTATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAGGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT GTCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCAGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGAG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGCATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCTGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CCGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGAC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCGA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGATGAA ACCTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGA TGTGCCCAAG GTGGGTGATC TCAGCTTTGT TGATTTTACG 
GACACCACGA TCGGAATTAG ATGGACCCCA CTAAAATACC AAGCAGTTAC TGGATACCAT 
ATTACAGTTG TTACGTCCGG CCAGAATTTT CCCATCTTGG AGGATGTGGT GAACTCCTCC 
GTCAACTATT ATACCATTCG TGGACTGGAG CCAGGCATCA ATTATGACAT CAGTGTATCC 
ACCATTACAG ACGAGGCGGA GAGCGTACCC TCAGTGATCA CACAGCAGAC CCAAACTGCT 
GTTCCAGCCC CCACAAACCT GTACATTTCT GAGGTGGGCG CAGACAGCAT GCATGTGTCA 
TGGACCGCAC CTTCTGTTCA GCCATCTGAA ATTAGTCGAT TTGTCATCCG CTACCATCCA 
ACGAATAATG ACGATGACAC TCAGGAAGTC AACGTAGGAG GTGGCACCAC TAGCTTCGTC 
CTTCAGAACT TGCTGCCAAA TACAGAGTAT CTGGTGAAAG TGGTGTGCGT CTATGATGAC 
AGAGAGAGTG AACCTGTAAC TGGTATCCAG AAAACAAAAC TGGACTCCCC AACAAATCTG 
GACTTCTCTG ACGTCTCCAC CAATTCATTC ACCGTGCACT GGATGGCCCC ACGTGCAGTC 
ATTACCGGTT ACCGCCTACG TTATCAACCA ACAAGTGGTG GGCGTGCTAA AGATGAGAGA 
CTTCCACCAA CAAGAAACTA CTTCACGCTG GTGAACCTGG CCCCTGAGAC AGAGTATACC 
GTTCATATTT ATGCCGTCAG CAGCAATATA GAAAGTCTAC CACTTACTGG AACACAAGCA 
ACTGTTTCTG ATGCTCCCAC TGACCTGGCT GTGACATCCT CCACACCCAC CAGCATTACC 
ATCTCCTGGG ATGCCCCGGC AGTTACCGTA CGCTATTACA AGATCACACA TGGCGAAACT 
GGTGAAAGAG ATGCACCGAG AGAGTTCACT GTTCCAGGAA CACAGTCTTC TGCCACCATC 
CAGGGTCTGC GGCCTGACAC AGATTACACC ATTACGCTGT ATGCTGTGAC AGGAAGAGGG 
GACAGCCCAG CATCCAGCAC ACCAGTTATC ATCACACACA GGACTGCGGG TGGAAGCATT 
GCGTCGCCAT CAGATTTAGA TGTTACAGAT ATTCAAGACA ATACCTTGAC TGTACGCTGG 
ACCCCAGCTA GAGGTCCCAT TACAGGGTAC AGGGTAACAG GGACACCCAA GGGTGGGCAA 
GGTCCTACAT TCTCTGAACT AGTTGGCCCT GAGCGTACAG AAATGACCAT CCATGGCTTG 
GTGCCCACGG TGGAGTATAC AATCAATGTG GTTGCTCTCA GCCAAGAAGG AGAAAGCACA 
CCAGTGGTCC AAAAAGCTAC AACAACTAAT GAACAGAAAC CAAAAGACCT GACCTTCACT 
GATGTGGACT CTACCTCCAT GCTTGTGAAA TGGAATGTTC CACGGGTTCC AGGAGTGACC 
TCCTACAGGG TTCTGTATTC TAGTCCGGAG GAAGGCGAAA GAGAGTACCA ACCTGCCTTA 
AGTGGACGTG ATAACAGTGT GCTTCTTCAA GGTCTGAACC CCGGAACCAT GTACATCATT 
AAAGTTATTC CAATGAAAGG ACGCAACCCA CTTCAAACCC TTGAGGGCAT ACAGCAAACT 
ATCCAAGAAG ACACTCAGCG CATTGCTGTT CCTGCTCCCA CCGATATTCA GTTCTTCGAC 
ATCAGTCCGT CTTCCTTTAT TGTGTCATGG CAAGCACCCA ATTCCAGACT TTCTGGCTAT 
CGTGTGGTGG TCACCCCAAG GAATCAGCTT GCCACATCCA AAGAAATGAC CATTTCACCA 
GACTCTACGC AAGTCTTAGT GCCTGGGCTT GTGGTGGCAA CCCCTTACAA TGTACATGTC 
TATGCACTGA GGGGTTCAGA CAGCAGTCCC CCTCTTACTG GAGAAATTAC CACCGCCGAC 
AATATTACCC CTCCTCGCCG AGTACGTATC AGTGATGTAA AAGACACCTC ATTCACTCTC 
GCATGGCGTG CCGTCAATAA TGAGCCAATG ACAGGATTCC TTATTGAAGC CACGCCCAAA 
AATGGAGGTT ACCCCACCTT TAGACAAACT ATTCCTTCTG ACAGACGTAC CTATGTTGTC 
ACTGGTTTGC ATCCAGGTGT CATGTATGTG GTCAACATGT ACACAGTAAA TGGAAACATG 
AGAAGTCCTC CTTTCACAAT GACTGTAAAC ACAGCACGTC CAGCTGTTCA GTCTCCAACC 
AACCTCCAAT TCACCTCCCT AACCCCTAAT TCCATCTCCT TCACTTGGCA AGCCCCTCCA 
ACACACATCA CAGGATATTA TATTACTTAT GAAGAATCAG GTGGCTCACC TCGTGAGCTT 
TTTCCACGCA ATAATGTTGG CCAGAACTAT GCCACTATAG CCGGTCTAAA ACCAGGCACT 
GAATACATTA TTAAGATCAT TGCCCTTCTG AATAACCAGA GAAGCGCACC ACTAGTTGGC 
ACAGCCACAA CTCAGCTGAT ATCCGGTCTA CCTAGCCTGC CCAGTCATGG TGGCCGAGAC 
AAGCTGGATG TGCCAGAGCC TGACAACAGA ATTAATACAG TGGGTCCTAC TGGTCATGAT 
TCAACGGATG CGCACGGGCA GCACGTGGAG TACACCGAAT ACAATAATCA GCCCAAACAA 
CCCAACATAG GTTACCAGCC TCAAAATCGT CAGCCAATCT CTCAACCTAA CCAACCTCGA 
CCACAGCCTT ATGTGCCTCA AGTTGGAGAG ACCCTAGTCT ATGTCCCCCA GGTGGGACCC 
GATGGGGGTC GTGTGCCTCA AATTATTCAG GTTAGTGAGA GGCCTGGCGA TGGCCATACT 
TTTGGTTTTC CAGAGGACAG GACAGGAAGG CCGCAGGAAG CTCAGACCCA GACTACCATC 
TCATGGCAGC CCTTCCAGCA GAGTTCTGCC TACCTGGTAT CATGTCAGCC AATCACCCAT 
CCAGATGAGA AGATGTTCCA GATGCGTGTC CCAGGAACAT CCACAACTGC CACTCTTATT 
GGTCTCACCT CTGGTGCGTC CTATAATGTC ATTGTGGAGG CCTTGAAAGG AGCCCTCAAG 
CACAAAATCC TAGAGGAAGT CGTCACTGCT GGAAATACAG TTCCAGGAGA TGCTACATCC 
AGCAAGGACT CGTGTTATGA CACTTTCACG GCCACTCACC ACGATGTCGG AGCGGAATGG 
GAGCGCATGT CTGATACAGG CTTTAAACTC TGGTGTAGAT GTCTTGGATT GGGAAGTGGA 
CATTTCAGAT GTGACTCTTC CAAATGGTGT CACGACAGCG GAAATAACTA CCGCATTGGC 
GAAAAGTGGG AGCGTCGGGC AGAAAACGGT CACTTGATGA GTTGCACTTG CTTGGGCAAT 
GGCAAAGGAG AGTTCAAGTG TGAACCACAT GACTCCATAT GTTACGATGA TGGAAAGATG 
TACCAGATTG GAAACCAGTG GCAGAAAGAA TACTTGGGGG CCATCTGCAC TTGCACATGC 
TATGGAGGAC AACAGGGTTG GCGATGTGAG AACTGCAGAA GGCCAGGTGC TGAGATAAAT 
GCTGACTTGC TTAGGCCTGT GCGCCTTAAC AACGGCCAAA GGGTGAATAT TCAGTGCCCC 
ATCGAATGCC TCCGACCAGA ACTTCTTGCA GATGCTGTAG TCAATCCCAA AACCCAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303829.1
CDS	115..7617
Translation	MTRESVKRLLLLLCIGGSVHCMPQSAGKSKRQAQQQQIHLDTVYEEARSLAIHESGCNDNGRVYRMNDEWERPYMDSTLKCTCEGASGVKCRSKPAAEESCYDKFNARSYRVGETYERPKDNMIWDCTCIGSGKGKISCTIANRCHEGGNSYRIGDTWTRPHDTGDYMLECVCLGNGKGEWTCKPVAERCYDDSLGSSYVVGQTWQKPYQGWMIVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGETWSKIDSSGHTLQCLCTGNGRGEWKCDRHAASHVIPAIGTGSAVTHRVTPVMNQVNVLNELIEEGNCKTDSGVSYYNGMSWIRTQGTKEMLCTCVGGGISCEEQDGQSQVYGGNSGGQPCVFPFVFSGNTHYSCISEGRSDGQLWCSTTSDYDSDGLYSFCTQKNLLVTTRGGNSNGALCQFPFKYNGRNYTDCTAEGRRDGMKWCGTTADYDREQKYGFCPMAAHEEVCTVNDVMYRVGDEWDKRHDTLGHMMRCTCQGNGRGEWNCISHTQLKDQCVVNGQTYDVNETFDKRHDQGYMMNCTCFGQGRGRWKCDAIDQCQEPETKVFYQIGQTWNKVIQGTPYRCSCYGNGIGEMACEPLQSTAPVRVIITEAGNQPNSHPIQWNAPPSAHITQYILKWRVKNTRTPWKEVTIPGHINSYTISGLKAGLTYEGQLISILRYGPREVTRFDFTTTYGSLAKAEGETTQPRQVVDTSESVTEITSNSFVISWVSASDTVSGFRVEYELSEEGAQKRVLDLPRTTTSVNIQDLLPGRRYNVDVYEVNPEGDTKLILTTTQTTAPDAPTNHEVSNVQETSIVIRWSKPKAPITGYRVVYTPSVEGSSTELILPETQTSVTLGDLRPGLSYNISIYSVEDNLESEPLVLQASTAGEQQPEEIQAPTDLQFYEVTDVKITITWTGPPNEVSGYRVTFAPVSTDGQAQRPLQLPVTHNAYAELMRLQPGTLYRFHIYAVSGGVESEPLIGEKSTKPDAPTDLRFTDITDDSALVIWSFPRAQVTGYRLFISIGSSSPKQLRIPGHKSQYKLPNLQPDTEYRVTLHSEQGNTLSEGITDTFRTTQPTGNAPRFNTEVTDTAIIVTWIPVAKFSYRMSVKPSQSGETPREETSGSGRIYISGLTPGLEYIYSLQPLFSGRKHGSPITNKVVTSLSPPTDLNVEPNSVNGELNVRWRGTKSPDITGYRVTGTPINGQRGVSLEESVRGDETSCILENLSPGVDYNISVYTVKNHLESEPISTSVTQDVPKVGDLSFVDFTDTTIGIRWTPLKYQAVTGYHITVVTSGQNFPILEDVVNSSVNYYTIRGLEPGINYDISVSTITDEAESVPSVITQQTQTAVPAPTNLYISEVGADSMHVSWTAPSVQPSEISRFVIRYHPTNNDDDTQEVNVGGGTTSFVLQNLLPNTEYLVKVVCVYDDRESEPVTGIQKTKLDSPTNLDFSDVSTNSFTVHWMAPRAVITGYRLRYQPTSGGRAKDERLPPTRNYFTLVNLAPETEYTVHIYAVSSNIESLPLTGTQATVSDAPTDLAVTSSTPTSITISWDAPAVTVRYYKITHGETGERDAPREFTVPGTQSSATIQGLRPDTDYTITLYAVTGRGDSPASSTPVIITHRTAGGSIASPSDLDVTDIQDNTLTVRWTPARGPITGYRVTGTPKGGQGPTFSELVGPERTEMTIHGLVPTVEYTINVVALSQEGESTPVVQKATTTNEQKPKDLTFTDVDSTSMLVKWNVPRVPGVTSYRVLYSSPEEGEREYQPALSGRDNSVLLQGLNPGTMYIIKVIPMKGRNPLQTLEGIQQTIQEDTQRIAVPAPTDIQFFDISPSSFIVSWQAPNSRLSGYRVVVTPRNQLATSKEMTISPDSTQVLVPGLVVATPYNVHVYALRGSDSSPPLTGEITTADNITPPRRVRISDVKDTSFTLAWRAVNNEPMTGFLIEATPKNGGYPTFRQTIPSDRRTYVVTGLHPGVMYVVNMYTVNGNMRSPPFTMTVNTARPAVQSPTNLQFTSLTPNSISFTWQAPPTHITGYYITYEESGGSPRELFPRNNVGQNYATIAGLKPGTEYIIKIIALLNNQRSAPLVGTATTQLISGLPSLPSHGGRDKLDVPEPDNRINTVGPTGHDSTDAHGQHVEYTEYNNQPKQPNIGYQPQNRQPISQPNQPRPQPYVPQVGETLVYVPQVGPDGGRVPQIIQVSERPGDGHTFGFPEDRTGRPQEAQTQTTISWQPFQQSSAYLVSCQPITHPDEKMFQMRVPGTSTTATLIGLTSGASYNVIVEALKGALKHKILEEVVTAGNTVPGDATSSKDSCYDTFTATHHDVGAEWERMSDTGFKLWCRCLGLGSGHFRCDSSKWCHDSGNNYRIGEKWERRAENGHLMSCTCLGNGKGEFKCEPHDSICYDDGKMYQIGNQWQKEYLGAICTCTCYGGQQGWRCENCRRPGAEINADLLRPVRLNNGQRVNIQCPIECLRPELLADAVVNPKTQE

Target ORF information:

RefSeq Version	XM_009305554.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio fibronectin 1b (fn1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305554.2

ORF Insert Sequence:

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG TAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGATA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTTGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGCGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC ACAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA CACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGAC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCCATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGTTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACTATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAGGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT GTCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCAGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGAG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGCATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCTGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CCGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGAC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCGA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGATGAA ACCTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGA TGTGCCCAAG GTGGGTGATC TCAGCTTTGT TGATTTTACG 
GACACCACGA TCGGAATTAG ATGGACCCCA CTAAAATACC AAGCAGTTAC TGGATACCAT 
ATTACAGTTG TTACGTCCGG CCAGAATTTT CCCATCTTGG AGGATGTGGT GAACTCCTCC 
GTCAACTATT ATACCATTCG TGGACTGGAG CCAGGCATCA ATTATGACAT CAGTGTATCC 
ACCATTACAG ACGAGGCGGA GAGCGTACCC TCAGTGATCA CACAGCAGAC CCAAACTGCT 
GTTCCAGCCC CCACAAACCT GTACATTTCT GAGGTGGGCG CAGACAGCAT GCATGTGTCA 
TGGACCGCAC CTTCTGTTCA GCCATCTGAA ATTAGTCGAT TTGTCATCCG CTACCATCCA 
ACGAATAATG ACGATGACAC TCAGGAAGTC AACGTAGGAG GTGGCACCAC TAGCTTCGTC 
CTTCAGAACT TGCTGCCAAA TACAGAGTAT CTGGTGAAAG TGGTGTGCGT CTATGATGAC 
AGAGAGAGTG AACCTGTAAC TGGTATCCAG AAAACAAAAC TGGACTCCCC AACAAATCTG 
GACTTCTCTG ACGTCTCCAC CAATTCATTC ACCGTGCACT GGATGGCCCC ACGTGCAGTC 
ATTACCGGTT ACCGCCTACG TTATCAACCA ACAAGTGGTG GGCGTGCTAA AGATGAGAGA 
CTTCCACCAA CAAGAAACTA CTTCACGCTG GTGAACCTGG CCCCTGAGAC AGAGTATACC 
GTTCATATTT ATGCCGTCAG CAGCAATATA GAAAGTCTAC CACTTACTGG AACACAAGCA 
ACTGTTTCTG ATGCTCCCAC TGACCTGGCT GTGACATCCT CCACACCCAC CAGCATTACC 
ATCTCCTGGG ATGCCCCGGC AGTTACCGTA CGCTATTACA AGATCACACA TGGCGAAACT 
GGTGAAAGAG ATGCACCGAG AGAGTTCACT GTTCCAGGAA CACAGTCTTC TGCCACCATC 
CAGGGTCTGC GGCCTGACAC AGATTACACC ATTACGCTGT ATGCTGTGAC AGGAAGAGGG 
GACAGCCCAG CATCCAGCAC ACCAGTTATC ATCACACACA GGACTGCGGG TGGAAGCATT 
GCGTCGCCAT CAGATTTAGA TGTTACAGAT ATTCAAGACA ATACCTTGAC TGTACGCTGG 
ACCCCAGCTA GAGGTCCCAT TACAGGGTAC AGGGTAACAG GGACACCCAA GGGTGGGCAA 
GGTCCTACAT TCTCTGAACT AGTTGGCCCT GAGCGTACAG AAATGACCAT CCATGGCTTG 
GTGCCCACGG TGGAGTATAC AATCAATGTG GTTGCTCTCA GCCAAGAAGG AGAAAGCACA 
CCAGTGGTCC AAAAAGCTAC AACAACTAAT GAACAGAAAC CAAAAGACCT GACCTTCACT 
GATGTGGACT CTACCTCCAT GCTTGTGAAA TGGAATGTTC CACGGGTTCC AGGAGTGACC 
TCCTACAGGG TTCTGTATTC TAGTCCGGAG GAAGGCGAAA GAGAGTACCA ACCTGCCTTA 
AGTGGACGTG ATAACAGTGT GCTTCTTCAA GGTCTGAACC CCGGAACCAT GTACATCATT 
AAAGTTATTC CAATGAAAGG ACGCAACCCA CTTCAAACCC TTGAGGGCAT ACAGCAAACT 
ATCCAAGAAG ACACTCAGCG CATTGCTGTT CCTGCTCCCA CCGATATTCA GTTCTTCGAC 
ATCAGTCCGT CTTCCTTTAT TGTGTCATGG CAAGCACCCA ATTCCAGACT TTCTGGCTAT 
CGTGTGGTGG TCACCCCAAG GAATCAGCTT GCCACATCCA AAGAAATGAC CATTTCACCA 
GACTCTACGC AAGTCTTAGT GCCTGGGCTT GTGGTGGCAA CCCCTTACAA TGTACATGTC 
TATGCACTGA GGGGTTCAGA CAGCAGTCCC CCTCTTACTG GAGAAATTAC CACCGCCGAC 
AATATTACCC CTCCTCGCCG AGTACGTATC AGTGATGTAA AAGACACCTC ATTCACTCTC 
GCATGGCGTG CCGTCAATAA TGAGCCAATG ACAGGATTCC TTATTGAAGC CACGCCCAAA 
AATGGAGGTT ACCCCACCTT TAGACAAACT ATTCCTTCTG ACAGACGTAC CTATGTTGTC 
ACTGGTTTGC ATCCAGGTGT CATGTATGTG GTCAACATGT ACACAGTAAA TGGAAACATG 
AGAAGTCCTC CTTTCACAAT GACTGTAAAC ACAGCACGTC CAGCTGTTCA GTCTCCAACC 
AACCTCCAAT TCACCTCCCT AACCCCTAAT TCCATCTCCT TCACTTGGCA AGCCCCTCCA 
ACACACATCA CAGGATATTA TATTACTTAT GAAGAATCAG GTGGCTCACC TCGTGAGCTT 
TTTCCACGCA ATAATGTTGG CCAGAACTAT GCCACTATAG CCGGTCTAAA ACCAGGCACT 
GAATACATTA TTAAGATCAT TGCCCTTCTG AATAACCAGA GAAGCGCACC ACTAGTTGGC 
ACAGCCACAA CTCAGCTGAT ATCCGGTCTA CCTAGCCTGC CCAGTCATGG TGGCCGAGAC 
AAGCTGGATG TGCCAGAGCC TGACAACAGA ATTAATACAG TGGGTCCTAC TGGTCATGAT 
TCAACGGATG CGCACGGGCA GCACGTGGAG TACACCGAAT ACAATAATCA GCCCAAACAA 
CCCAACATAG GTTACCAGCC TCAAAATCGT CAGCCAATCT CTCAACCTAA CCAACCTCGA 
CCACAGCCTT ATGTGCCTCA AGTTGGAGAG ACCCTAGTCT ATGTCCCCCA GGTGGGACCC 
GATGGGGGTC GTGTGCCTCA AATTATTCAG GTTAGTGAGA GGCCTGGCGA TGGCCATACT 
TTTGGTTTTC CAGAGGACAG GACAGGAAGG CCGCAGGAAG CTCAGACCCA GACTACCATC 
TCATGGCAGC CCTTCCAGCA GAGTTCTGCC TACCTGGTAT CATGTCAGCC AATCACCCAT 
CCAGATGAGA AGATGTTCCA GATGCGTGTC CCAGGAACAT CCACAACTGC CACTCTTATT 
GGTCTCACCT CTGGTGCGTC CTATAATGTC ATTGTGGAGG CCTTGAAAGG AGCCCTCAAG 
CACAAAATCC TAGAGGAAGT CGTCACTGCT GGAAATACAG TTCCAGGAGA TGCTACATCC 
AGCAAGGACT CGTGTTATGA CACTTTCACG GCCACTCACC ACGATGTCGG AGCGGAATGG 
GAGCGCATGT CTGATACAGG CTTTAAACTC TGGTGTAGAT GTCTTGGATT GGGAAGTGGA 
CATTTCAGAT GTGACTCTTC CAAATGGTGT CACGACAGCG GAAATAACTA CCGCATTGGC 
GAAAAGTGGG AGCGTCGGGC AGAAAACGGT CACTTGATGA GTTGCACTTG CTTGGGCAAT 
GGCAAAGGAG AGTTCAAGTG TGAACCACAT GACTCCATAT GTTACGATGA TGGAAAGATG 
TACCAGATTG GAAACCAGTG GCAGAAAGAA TACTTGGGGG CCATCTGCAC TTGCACATGC 
TATGGAGGAC AACAGGGTTG GCGATGTGAG AACTGCAGAA GGCCAGGTGC TGAGATAAAT 
GCTGACTTGC TTAGGCCTGT GCGCCTTAAC AACGGCCAAA GGGTGAATAT TCAGTGCCCC 
ATCGAATGCC TCCGACCAGA ACTTCTTGCA GATGCTGTAG TCAATCCCAA AACCCAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fn1b ( XM_009305554.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303829.1 Danio rerio fibronectin 1b (fn1b), transcript variant X2, mRNA.

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CloneID	ODa40007
Clone ID Related Accession (Same CDS sequence)	XM_009305554.2 , XM_009305554.3
Accession Version	XM_009305554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	fibronectin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG TAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGATA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTTGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGCGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC ACAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA CACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGAC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCCATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGTTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACTATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAGGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT GTCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCAGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGAG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGCATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCTGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CCGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGAC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCGA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGATGAA ACCTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGA TGTGCCCAAG GTGGGTGATC TCAGCTTTGT TGATTTTACG 
GACACCACGA TCGGAATTAG ATGGACCCCA CTAAAATACC AAGCAGTTAC TGGATACCAT 
ATTACAGTTG TTACGTCCGG CCAGAATTTT CCCATCTTGG AGGATGTGGT GAACTCCTCC 
GTCAACTATT ATACCATTCG TGGACTGGAG CCAGGCATCA ATTATGACAT CAGTGTATCC 
ACCATTACAG ACGAGGCGGA GAGCGTACCC TCAGTGATCA CACAGCAGAC CCAAACTGCT 
GTTCCAGCCC CCACAAACCT GTACATTTCT GAGGTGGGCG CAGACAGCAT GCATGTGTCA 
TGGACCGCAC CTTCTGTTCA GCCATCTGAA ATTAGTCGAT TTGTCATCCG CTACCATCCA 
ACGAATAATG ACGATGACAC TCAGGAAGTC AACGTAGGAG GTGGCACCAC TAGCTTCGTC 
CTTCAGAACT TGCTGCCAAA TACAGAGTAT CTGGTGAAAG TGGTGTGCGT CTATGATGAC 
AGAGAGAGTG AACCTGTAAC TGGTATCCAG AAAACAAAAC TGGACTCCCC AACAAATCTG 
GACTTCTCTG ACGTCTCCAC CAATTCATTC ACCGTGCACT GGATGGCCCC ACGTGCAGTC 
ATTACCGGTT ACCGCCTACG TTATCAACCA ACAAGTGGTG GGCGTGCTAA AGATGAGAGA 
CTTCCACCAA CAAGAAACTA CTTCACGCTG GTGAACCTGG CCCCTGAGAC AGAGTATACC 
GTTCATATTT ATGCCGTCAG CAGCAATATA GAAAGTCTAC CACTTACTGG AACACAAGCA 
ACTGTTTCTG ATGCTCCCAC TGACCTGGCT GTGACATCCT CCACACCCAC CAGCATTACC 
ATCTCCTGGG ATGCCCCGGC AGTTACCGTA CGCTATTACA AGATCACACA TGGCGAAACT 
GGTGAAAGAG ATGCACCGAG AGAGTTCACT GTTCCAGGAA CACAGTCTTC TGCCACCATC 
CAGGGTCTGC GGCCTGACAC AGATTACACC ATTACGCTGT ATGCTGTGAC AGGAAGAGGG 
GACAGCCCAG CATCCAGCAC ACCAGTTATC ATCACACACA GGACTGCGGG TGGAAGCATT 
GCGTCGCCAT CAGATTTAGA TGTTACAGAT ATTCAAGACA ATACCTTGAC TGTACGCTGG 
ACCCCAGCTA GAGGTCCCAT TACAGGGTAC AGGGTAACAG GGACACCCAA GGGTGGGCAA 
GGTCCTACAT TCTCTGAACT AGTTGGCCCT GAGCGTACAG AAATGACCAT CCATGGCTTG 
GTGCCCACGG TGGAGTATAC AATCAATGTG GTTGCTCTCA GCCAAGAAGG AGAAAGCACA 
CCAGTGGTCC AAAAAGCTAC AACAACTAAT GAACAGAAAC CAAAAGACCT GACCTTCACT 
GATGTGGACT CTACCTCCAT GCTTGTGAAA TGGAATGTTC CACGGGTTCC AGGAGTGACC 
TCCTACAGGG TTCTGTATTC TAGTCCGGAG GAAGGCGAAA GAGAGTACCA ACCTGCCTTA 
AGTGGACGTG ATAACAGTGT GCTTCTTCAA GGTCTGAACC CCGGAACCAT GTACATCATT 
AAAGTTATTC CAATGAAAGG ACGCAACCCA CTTCAAACCC TTGAGGGCAT ACAGCAAACT 
ATCCAAGAAG ACACTCAGCG CATTGCTGTT CCTGCTCCCA CCGATATTCA GTTCTTCGAC 
ATCAGTCCGT CTTCCTTTAT TGTGTCATGG CAAGCACCCA ATTCCAGACT TTCTGGCTAT 
CGTGTGGTGG TCACCCCAAG GAATCAGCTT GCCACATCCA AAGAAATGAC CATTTCACCA 
GACTCTACGC AAGTCTTAGT GCCTGGGCTT GTGGTGGCAA CCCCTTACAA TGTACATGTC 
TATGCACTGA GGGGTTCAGA CAGCAGTCCC CCTCTTACTG GAGAAATTAC CACCGCCGAC 
AATATTACCC CTCCTCGCCG AGTACGTATC AGTGATGTAA AAGACACCTC ATTCACTCTC 
GCATGGCGTG CCGTCAATAA TGAGCCAATG ACAGGATTCC TTATTGAAGC CACGCCCAAA 
AATGGAGGTT ACCCCACCTT TAGACAAACT ATTCCTTCTG ACAGACGTAC CTATGTTGTC 
ACTGGTTTGC ATCCAGGTGT CATGTATGTG GTCAACATGT ACACAGTAAA TGGAAACATG 
AGAAGTCCTC CTTTCACAAT GACTGTAAAC ACAGCACGTC CAGCTGTTCA GTCTCCAACC 
AACCTCCAAT TCACCTCCCT AACCCCTAAT TCCATCTCCT TCACTTGGCA AGCCCCTCCA 
ACACACATCA CAGGATATTA TATTACTTAT GAAGAATCAG GTGGCTCACC TCGTGAGCTT 
TTTCCACGCA ATAATGTTGG CCAGAACTAT GCCACTATAG CCGGTCTAAA ACCAGGCACT 
GAATACATTA TTAAGATCAT TGCCCTTCTG AATAACCAGA GAAGCGCACC ACTAGTTGGC 
ACAGCCACAA CTCAGCTGAT ATCCGGTCTA CCTAGCCTGC CCAGTCATGG TGGCCGAGAC 
AAGCTGGATG TGCCAGAGCC TGACAACAGA ATTAATACAG TGGGTCCTAC TGGTCATGAT 
TCAACGGATG CGCACGGGCA GCACGTGGAG TACACCGAAT ACAATAATCA GCCCAAACAA 
CCCAACATAG GTTACCAGCC TCAAAATCGT CAGCCAATCT CTCAACCTAA CCAACCTCGA 
CCACAGCCTT ATGTGCCTCA AGTTGGAGAG ACCCTAGTCT ATGTCCCCCA GGTGGGACCC 
GATGGGGGTC GTGTGCCTCA AATTATTCAG GTTAGTGAGA GGCCTGGCGA TGGCCATACT 
TTTGGTTTTC CAGAGGACAG GACAGGAAGG CCGCAGGAAG CTCAGACCCA GACTACCATC 
TCATGGCAGC CCTTCCAGCA GAGTTCTGCC TACCTGGTAT CATGTCAGCC AATCACCCAT 
CCAGATGAGA AGATGTTCCA GATGCGTGTC CCAGGAACAT CCACAACTGC CACTCTTATT 
GGTCTCACCT CTGGTGCGTC CTATAATGTC ATTGTGGAGG CCTTGAAAGG AGCCCTCAAG 
CACAAAATCC TAGAGGAAGT CGTCACTGCT GGAAATACAG TTCCAGGAGA TGCTACATCC 
AGCAAGGACT CGTGTTATGA CACTTTCACG GCCACTCACC ACGATGTCGG AGCGGAATGG 
GAGCGCATGT CTGATACAGG CTTTAAACTC TGGTGTAGAT GTCTTGGATT GGGAAGTGGA 
CATTTCAGAT GTGACTCTTC CAAATGGTGT CACGACAGCG GAAATAACTA CCGCATTGGC 
GAAAAGTGGG AGCGTCGGGC AGAAAACGGT CACTTGATGA GTTGCACTTG CTTGGGCAAT 
GGCAAAGGAG AGTTCAAGTG TGAACCACAT GACTCCATAT GTTACGATGA TGGAAAGATG 
TACCAGATTG GAAACCAGTG GCAGAAAGAA TACTTGGGGG CCATCTGCAC TTGCACATGC 
TATGGAGGAC AACAGGGTTG GCGATGTGAG AACTGCAGAA GGCCAGGTGC TGAGATAAAT 
GCTGACTTGC TTAGGCCTGT GCGCCTTAAC AACGGCCAAA GGGTGAATAT TCAGTGCCCC 
ATCGAATGCC TCCGACCAGA ACTTCTTGCA GATGCTGTAG TCAATCCCAA AACCCAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303829.1
CDS	110..7612
Translation	MTRESVKRLLLLLCIGGSVHCMPQSAGKSKRQAQQQQIHLDTVYEEARSLAIHESGCNDNGRVYRMNDEWERPYMDSTLKCTCEGASGVKCRSKPAAEESCYDKFNARSYRVGETYERPKDNMIWDCTCIGSGKGKISCTIANRCHEGGNSYRIGDTWTRPHDTGDYMLECVCLGNGKGEWTCKPVAERCYDDSLGSSYVVGQTWQKPYQGWMIVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGETWSKIDSSGHTLQCLCTGNGRGEWKCDRHAASHVIPAIGTGSAVTHRVTPVMNQVNVLNELIEEGNCKTDSGVSYYNGMSWIRTQGTKEMLCTCVGGGISCEEQDGQSQVYGGNSGGQPCVFPFVFSGNTHYSCISEGRSDGQLWCSTTSDYDSDGLYSFCTQKNLLVTTRGGNSNGALCQFPFKYNGRNYTDCTAEGRRDGMKWCGTTADYDREQKYGFCPMAAHEEVCTVNDVMYRVGDEWDKRHDTLGHMMRCTCQGNGRGEWNCISHTQLKDQCVVNGQTYDVNETFDKRHDQGYMMNCTCFGQGRGRWKCDAIDQCQEPETKVFYQIGQTWNKVIQGTPYRCSCYGNGIGEMACEPLQSTAPVRVIITEAGNQPNSHPIQWNAPPSAHITQYILKWRVKNTRTPWKEVTIPGHINSYTISGLKAGLTYEGQLISILRYGPREVTRFDFTTTYGSLAKAEGETTQPRQVVDTSESVTEITSNSFVISWVSASDTVSGFRVEYELSEEGAQKRVLDLPRTTTSVNIQDLLPGRRYNVDVYEVNPEGDTKLILTTTQTTAPDAPTNHEVSNVQETSIVIRWSKPKAPITGYRVVYTPSVEGSSTELILPETQTSVTLGDLRPGLSYNISIYSVEDNLESEPLVLQASTAGEQQPEEIQAPTDLQFYEVTDVKITITWTGPPNEVSGYRVTFAPVSTDGQAQRPLQLPVTHNAYAELMRLQPGTLYRFHIYAVSGGVESEPLIGEKSTKPDAPTDLRFTDITDDSALVIWSFPRAQVTGYRLFISIGSSSPKQLRIPGHKSQYKLPNLQPDTEYRVTLHSEQGNTLSEGITDTFRTTQPTGNAPRFNTEVTDTAIIVTWIPVAKFSYRMSVKPSQSGETPREETSGSGRIYISGLTPGLEYIYSLQPLFSGRKHGSPITNKVVTSLSPPTDLNVEPNSVNGELNVRWRGTKSPDITGYRVTGTPINGQRGVSLEESVRGDETSCILENLSPGVDYNISVYTVKNHLESEPISTSVTQDVPKVGDLSFVDFTDTTIGIRWTPLKYQAVTGYHITVVTSGQNFPILEDVVNSSVNYYTIRGLEPGINYDISVSTITDEAESVPSVITQQTQTAVPAPTNLYISEVGADSMHVSWTAPSVQPSEISRFVIRYHPTNNDDDTQEVNVGGGTTSFVLQNLLPNTEYLVKVVCVYDDRESEPVTGIQKTKLDSPTNLDFSDVSTNSFTVHWMAPRAVITGYRLRYQPTSGGRAKDERLPPTRNYFTLVNLAPETEYTVHIYAVSSNIESLPLTGTQATVSDAPTDLAVTSSTPTSITISWDAPAVTVRYYKITHGETGERDAPREFTVPGTQSSATIQGLRPDTDYTITLYAVTGRGDSPASSTPVIITHRTAGGSIASPSDLDVTDIQDNTLTVRWTPARGPITGYRVTGTPKGGQGPTFSELVGPERTEMTIHGLVPTVEYTINVVALSQEGESTPVVQKATTTNEQKPKDLTFTDVDSTSMLVKWNVPRVPGVTSYRVLYSSPEEGEREYQPALSGRDNSVLLQGLNPGTMYIIKVIPMKGRNPLQTLEGIQQTIQEDTQRIAVPAPTDIQFFDISPSSFIVSWQAPNSRLSGYRVVVTPRNQLATSKEMTISPDSTQVLVPGLVVATPYNVHVYALRGSDSSPPLTGEITTADNITPPRRVRISDVKDTSFTLAWRAVNNEPMTGFLIEATPKNGGYPTFRQTIPSDRRTYVVTGLHPGVMYVVNMYTVNGNMRSPPFTMTVNTARPAVQSPTNLQFTSLTPNSISFTWQAPPTHITGYYITYEESGGSPRELFPRNNVGQNYATIAGLKPGTEYIIKIIALLNNQRSAPLVGTATTQLISGLPSLPSHGGRDKLDVPEPDNRINTVGPTGHDSTDAHGQHVEYTEYNNQPKQPNIGYQPQNRQPISQPNQPRPQPYVPQVGETLVYVPQVGPDGGRVPQIIQVSERPGDGHTFGFPEDRTGRPQEAQTQTTISWQPFQQSSAYLVSCQPITHPDEKMFQMRVPGTSTTATLIGLTSGASYNVIVEALKGALKHKILEEVVTAGNTVPGDATSSKDSCYDTFTATHHDVGAEWERMSDTGFKLWCRCLGLGSGHFRCDSSKWCHDSGNNYRIGEKWERRAENGHLMSCTCLGNGKGEFKCEPHDSICYDDGKMYQIGNQWQKEYLGAICTCTCYGGQQGWRCENCRRPGAEINADLLRPVRLNNGQRVNIQCPIECLRPELLADAVVNPKTQE

Target ORF information:

RefSeq Version	XM_009305554.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fibronectin 1b (fn1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305554.3

ORF Insert Sequence:

ATGACCCGTG AGTCAGTAAA GAGACTCCTG CTGCTGCTGT GCATCGGCGG ATCTGTCCAC 
TGCATGCCAC AATCCGCAGG GAAAAGTAAA AGACAAGCCC AGCAGCAGCA AATCCACCTG 
GACACAGTTT ACGAAGAGGC CAGGAGTTTA GCCATCCACG AAAGTGGCTG TAATGACAAC 
GGTCGTGTTT ACAGGATGAA CGATGAGTGG GAGCGGCCCT ACATGGACAG CACTCTCAAG 
TGCACTTGTG AGGGAGCTTC AGGGGTCAAG TGTAGATCCA AACCTGCAGC TGAGGAGTCA 
TGCTATGATA AATTCAACGC ACGTTCCTAC CGAGTCGGAG AGACCTACGA GCGACCAAAG 
GATAACATGA TATGGGACTG TACATGTATT GGGTCAGGCA AGGGCAAAAT TAGCTGCACC 
ATTGCAAATC GCTGCCATGA AGGGGGGAAT TCATACAGAA TTGGGGACAC CTGGACACGA 
CCACACGACA CTGGAGACTA CATGCTTGAA TGTGTTTGCC TTGGCAATGG AAAGGGAGAG 
TGGACTTGTA AACCTGTTGC GGAGCGTTGC TATGATGACT CACTGGGATC TTCATATGTG 
GTTGGTCAGA CATGGCAGAA ACCATATCAA GGTTGGATGA TCGTGGACTG CACATGTTTG 
GGAGAGGGAA ACGGACGCAT CACCTGCACC TCCAGAAACC GCTGCAATGA CCAGGACACC 
AGAACCTCAT ACCGTATTGG TGAAACCTGG AGCAAAATTG ACTCCAGCGG ACACACTCTG 
CAGTGTCTTT GCACAGGGAA CGGTCGTGGG GAGTGGAAGT GTGACAGACA TGCTGCTTCC 
CATGTAATTC CTGCCATTGG TACTGGATCT GCAGTGACTC ACAGAGTTAC ACCAGTGATG 
AACCAAGTCA ACGTCCTGAA TGAACTTATC GAAGAGGGCA ACTGCAAGAC CGATTCTGGG 
GTGTCCTATT ACAATGGCAT GAGCTGGATC AGAACTCAGG GCACCAAGGA GATGCTGTGC 
ACTTGTGTTG GAGGAGGAAT CAGCTGCGAG GAGCAAGACG GACAGTCCCA GGTATATGGT 
GGTAACTCTG GTGGGCAGCC ATGTGTGTTC CCCTTTGTTT TTAGTGGAAA CACCCACTAC 
TCCTGCATCT CCGAGGGTCG ATCTGATGGG CAGCTATGGT GCAGTACCAC CTCTGATTAT 
GACAGCGATG GCTTGTACTC ATTCTGTACC CAGAAGAATC TGCTTGTGAC AACCCGTGGA 
GGAAACTCGA ACGGTGCTCT TTGCCAATTC CCCTTCAAGT ATAATGGACG CAACTACACC 
GATTGCACAG CCGAGGGGCG TCGTGATGGC ATGAAGTGGT GCGGTACCAC TGCTGACTAT 
GACAGAGAGC AGAAATACGG TTTCTGTCCC ATGGCTGCTC ACGAGGAAGT CTGCACTGTT 
AATGATGTCA TGTACCGAGT CGGTGACGAG TGGGACAAGC GCCATGATAC TCTTGGTCAC 
ATGATGCGCT GTACGTGCCA GGGAAATGGA CGTGGAGAGT GGAACTGCAT TTCTCACACA 
CAGCTCAAAG ACCAGTGTGT TGTGAATGGA CAGACGTATG ATGTGAACGA GACGTTTGAT 
AAGCGCCATG ACCAGGGCTA CATGATGAAC TGCACGTGCT TTGGCCAAGG CCGTGGTCGC 
TGGAAATGTG ATGCCATTGA CCAGTGCCAG GAGCCTGAGA CCAAGGTGTT CTACCAGATT 
GGCCAAACAT GGAATAAAGT CATCCAAGGC ACCCCATACA GATGTTCCTG CTATGGTAAT 
GGCATTGGAG AAATGGCCTG TGAGCCTCTG CAGTCTACTG CTCCTGTTCG GGTGATCATC 
ACTGAAGCTG GAAATCAGCC CAATTCCCAT CCAATTCAAT GGAACGCTCC TCCGTCTGCC 
CACATTACGC AATACATCCT GAAGTGGAGA GTCAAAAATA CACGCACACC ATGGAAGGAG 
GTCACTATTC CTGGCCACAT CAACTCCTAC ACCATTTCAG GCCTCAAAGC AGGTTTGACG 
TATGAAGGCC AGTTGATCAG CATTCTGCGC TACGGACCAA GAGAGGTTAC TCGGTTTGAC 
TTCACCACCA CATATGGCTC TCTGGCCAAA GCAGAGGGTG AGACCACCCA GCCTCGACAA 
GTAGTGGACA CCTCAGAGTC TGTTACTGAA ATTACCTCCA ACAGCTTTGT CATCTCCTGG 
GTATCTGCAT CTGACACAGT GTCTGGATTT AGAGTTGAAT ATGAACTCTC AGAAGAAGGA 
GCTCAGAAAC GTGTGCTTGA CCTCCCAAGA ACAACTACAT CAGTGAACAT TCAGGATCTT 
CTGCCTGGCC GTAGGTACAA CGTTGATGTT TATGAAGTCA ATCCAGAAGG AGATACAAAA 
CTCATCCTGA CTACAACTCA AACCACTGCA CCTGATGCTC CAACCAATCA TGAGGTCTCA 
AATGTCCAGG AGACCTCCAT TGTGATCAGA TGGTCCAAAC CCAAAGCACC CATTACTGGC 
TATCGTGTGG TGTACACACC CTCAGTCGAG GGCAGCAGCA CTGAGCTCAT CCTCCCTGAG 
ACTCAGACAT CGGTGACACT GGGTGACCTA CGACCTGGCC TGTCATACAA TATCAGCATT 
TACTCAGTGG AAGACAACTT GGAGAGCGAA CCTTTGGTCC TGCAGGCAAG CACAGCTGGA 
GAGCAACAGC CCGAGGAAAT CCAAGCTCCC ACAGACCTGC AGTTTTATGA GGTTACCGAT 
GTCAAGATCA CTATCACCTG GACTGGTCCT CCTAATGAGG TCTCAGGATA CCGTGTGACT 
TTTGCACCCG TAAGCACAGA TGGCCAGGCC CAGAGACCCC TTCAGCTTCC TGTGACACAC 
AATGCTTATG CGGAGCTCAT GCGCCTTCAG CCTGGCACAC TCTACCGTTT CCATATTTAT 
GCAGTCAGCG GAGGAGTGGA GAGTGAACCT CTGATCGGGG AGAAATCTAC AAAGCCTGAT 
GCCCCTACTG ACTTGCGCTT CACTGATATC ACCGATGACA GCGCTCTGGT CATCTGGTCT 
TTCCCCAGAG CTCAGGTCAC AGGTTATCGT CTCTTCATCA GCATCGGCAG CTCCAGTCCC 
AAACAGCTGA GAATTCCTGG CCACAAGTCT CAATACAAGC TCCCCAACCT TCAGCCTGAC 
ACCGAGTATA GAGTCACCCT GCACTCAGAG CAAGGAAACA CTCTTAGCGA AGGAATTACT 
GATACTTTCA GAACAACTCA GCCCACGGGT AATGCTCCTC GATTCAACAC TGAAGTCACT 
GACACTGCTA TCATCGTCAC CTGGATCCCT GTTGCAAAAT TCAGTTACAG GATGTCTGTG 
AAGCCTAGCC AAAGTGGTGA AACTCCCAGA GAAGAGACTT CTGGCTCAGG CCGGATTTAC 
ATTTCTGGAC TGACTCCAGG ACTGGAGTAC ATCTACAGCT TGCAACCCTT GTTCAGTGGC 
CGAAAACATG GCAGCCCTAT CACAAACAAA GTTGTCACAT CCTTATCCCC ACCAACTGAT 
CTAAATGTGG AGCCTAACTC AGTCAACGGT GAACTCAATG TCAGATGGAG AGGCACCAAA 
AGTCCAGATA TCACTGGCTA CAGAGTGACA GGCACACCGA TCAATGGGCA GCGTGGAGTT 
AGTCTAGAGG AGTCTGTACG AGGGGATGAA ACCTCATGCA TTCTGGAGAA CCTGAGTCCT 
GGTGTTGATT ACAATATCAG TGTCTACACT GTAAAAAACC ACCTGGAGAG TGAGCCAATC 
TCGACTTCTG TCACGCAAGA TGTGCCCAAG GTGGGTGATC TCAGCTTTGT TGATTTTACG 
GACACCACGA TCGGAATTAG ATGGACCCCA CTAAAATACC AAGCAGTTAC TGGATACCAT 
ATTACAGTTG TTACGTCCGG CCAGAATTTT CCCATCTTGG AGGATGTGGT GAACTCCTCC 
GTCAACTATT ATACCATTCG TGGACTGGAG CCAGGCATCA ATTATGACAT CAGTGTATCC 
ACCATTACAG ACGAGGCGGA GAGCGTACCC TCAGTGATCA CACAGCAGAC CCAAACTGCT 
GTTCCAGCCC CCACAAACCT GTACATTTCT GAGGTGGGCG CAGACAGCAT GCATGTGTCA 
TGGACCGCAC CTTCTGTTCA GCCATCTGAA ATTAGTCGAT TTGTCATCCG CTACCATCCA 
ACGAATAATG ACGATGACAC TCAGGAAGTC AACGTAGGAG GTGGCACCAC TAGCTTCGTC 
CTTCAGAACT TGCTGCCAAA TACAGAGTAT CTGGTGAAAG TGGTGTGCGT CTATGATGAC 
AGAGAGAGTG AACCTGTAAC TGGTATCCAG AAAACAAAAC TGGACTCCCC AACAAATCTG 
GACTTCTCTG ACGTCTCCAC CAATTCATTC ACCGTGCACT GGATGGCCCC ACGTGCAGTC 
ATTACCGGTT ACCGCCTACG TTATCAACCA ACAAGTGGTG GGCGTGCTAA AGATGAGAGA 
CTTCCACCAA CAAGAAACTA CTTCACGCTG GTGAACCTGG CCCCTGAGAC AGAGTATACC 
GTTCATATTT ATGCCGTCAG CAGCAATATA GAAAGTCTAC CACTTACTGG AACACAAGCA 
ACTGTTTCTG ATGCTCCCAC TGACCTGGCT GTGACATCCT CCACACCCAC CAGCATTACC 
ATCTCCTGGG ATGCCCCGGC AGTTACCGTA CGCTATTACA AGATCACACA TGGCGAAACT 
GGTGAAAGAG ATGCACCGAG AGAGTTCACT GTTCCAGGAA CACAGTCTTC TGCCACCATC 
CAGGGTCTGC GGCCTGACAC AGATTACACC ATTACGCTGT ATGCTGTGAC AGGAAGAGGG 
GACAGCCCAG CATCCAGCAC ACCAGTTATC ATCACACACA GGACTGCGGG TGGAAGCATT 
GCGTCGCCAT CAGATTTAGA TGTTACAGAT ATTCAAGACA ATACCTTGAC TGTACGCTGG 
ACCCCAGCTA GAGGTCCCAT TACAGGGTAC AGGGTAACAG GGACACCCAA GGGTGGGCAA 
GGTCCTACAT TCTCTGAACT AGTTGGCCCT GAGCGTACAG AAATGACCAT CCATGGCTTG 
GTGCCCACGG TGGAGTATAC AATCAATGTG GTTGCTCTCA GCCAAGAAGG AGAAAGCACA 
CCAGTGGTCC AAAAAGCTAC AACAACTAAT GAACAGAAAC CAAAAGACCT GACCTTCACT 
GATGTGGACT CTACCTCCAT GCTTGTGAAA TGGAATGTTC CACGGGTTCC AGGAGTGACC 
TCCTACAGGG TTCTGTATTC TAGTCCGGAG GAAGGCGAAA GAGAGTACCA ACCTGCCTTA 
AGTGGACGTG ATAACAGTGT GCTTCTTCAA GGTCTGAACC CCGGAACCAT GTACATCATT 
AAAGTTATTC CAATGAAAGG ACGCAACCCA CTTCAAACCC TTGAGGGCAT ACAGCAAACT 
ATCCAAGAAG ACACTCAGCG CATTGCTGTT CCTGCTCCCA CCGATATTCA GTTCTTCGAC 
ATCAGTCCGT CTTCCTTTAT TGTGTCATGG CAAGCACCCA ATTCCAGACT TTCTGGCTAT 
CGTGTGGTGG TCACCCCAAG GAATCAGCTT GCCACATCCA AAGAAATGAC CATTTCACCA 
GACTCTACGC AAGTCTTAGT GCCTGGGCTT GTGGTGGCAA CCCCTTACAA TGTACATGTC 
TATGCACTGA GGGGTTCAGA CAGCAGTCCC CCTCTTACTG GAGAAATTAC CACCGCCGAC 
AATATTACCC CTCCTCGCCG AGTACGTATC AGTGATGTAA AAGACACCTC ATTCACTCTC 
GCATGGCGTG CCGTCAATAA TGAGCCAATG ACAGGATTCC TTATTGAAGC CACGCCCAAA 
AATGGAGGTT ACCCCACCTT TAGACAAACT ATTCCTTCTG ACAGACGTAC CTATGTTGTC 
ACTGGTTTGC ATCCAGGTGT CATGTATGTG GTCAACATGT ACACAGTAAA TGGAAACATG 
AGAAGTCCTC CTTTCACAAT GACTGTAAAC ACAGCACGTC CAGCTGTTCA GTCTCCAACC 
AACCTCCAAT TCACCTCCCT AACCCCTAAT TCCATCTCCT TCACTTGGCA AGCCCCTCCA 
ACACACATCA CAGGATATTA TATTACTTAT GAAGAATCAG GTGGCTCACC TCGTGAGCTT 
TTTCCACGCA ATAATGTTGG CCAGAACTAT GCCACTATAG CCGGTCTAAA ACCAGGCACT 
GAATACATTA TTAAGATCAT TGCCCTTCTG AATAACCAGA GAAGCGCACC ACTAGTTGGC 
ACAGCCACAA CTCAGCTGAT ATCCGGTCTA CCTAGCCTGC CCAGTCATGG TGGCCGAGAC 
AAGCTGGATG TGCCAGAGCC TGACAACAGA ATTAATACAG TGGGTCCTAC TGGTCATGAT 
TCAACGGATG CGCACGGGCA GCACGTGGAG TACACCGAAT ACAATAATCA GCCCAAACAA 
CCCAACATAG GTTACCAGCC TCAAAATCGT CAGCCAATCT CTCAACCTAA CCAACCTCGA 
CCACAGCCTT ATGTGCCTCA AGTTGGAGAG ACCCTAGTCT ATGTCCCCCA GGTGGGACCC 
GATGGGGGTC GTGTGCCTCA AATTATTCAG GTTAGTGAGA GGCCTGGCGA TGGCCATACT 
TTTGGTTTTC CAGAGGACAG GACAGGAAGG CCGCAGGAAG CTCAGACCCA GACTACCATC 
TCATGGCAGC CCTTCCAGCA GAGTTCTGCC TACCTGGTAT CATGTCAGCC AATCACCCAT 
CCAGATGAGA AGATGTTCCA GATGCGTGTC CCAGGAACAT CCACAACTGC CACTCTTATT 
GGTCTCACCT CTGGTGCGTC CTATAATGTC ATTGTGGAGG CCTTGAAAGG AGCCCTCAAG 
CACAAAATCC TAGAGGAAGT CGTCACTGCT GGAAATACAG TTCCAGGAGA TGCTACATCC 
AGCAAGGACT CGTGTTATGA CACTTTCACG GCCACTCACC ACGATGTCGG AGCGGAATGG 
GAGCGCATGT CTGATACAGG CTTTAAACTC TGGTGTAGAT GTCTTGGATT GGGAAGTGGA 
CATTTCAGAT GTGACTCTTC CAAATGGTGT CACGACAGCG GAAATAACTA CCGCATTGGC 
GAAAAGTGGG AGCGTCGGGC AGAAAACGGT CACTTGATGA GTTGCACTTG CTTGGGCAAT 
GGCAAAGGAG AGTTCAAGTG TGAACCACAT GACTCCATAT GTTACGATGA TGGAAAGATG 
TACCAGATTG GAAACCAGTG GCAGAAAGAA TACTTGGGGG CCATCTGCAC TTGCACATGC 
TATGGAGGAC AACAGGGTTG GCGATGTGAG AACTGCAGAA GGCCAGGTGC TGAGATAAAT 
GCTGACTTGC TTAGGCCTGT GCGCCTTAAC AACGGCCAAA GGGTGAATAT TCAGTGCCCC 
ATCGAATGCC TCCGACCAGA ACTTCTTGCA GATGCTGTAG TCAATCCCAA AACCCAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Yap is required for scar formation but not myocyte proliferation during heart regeneration in zebrafish.
Cardiovascular research115(3)570-577(2019 03)
Flinn MA,Jeffery BE,O'Meara CC,Link BA

The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling.
eLife7(2018 12)
Rasouli SJ,El-Brolosy M,Tsedeke AT,Bensimon-Brito A,Ghanbari P,Maischein HM,Kuenne C,Stainier DY

Vangl2-dependent regulation of membrane protrusions and directed migration requires a fibronectin extracellular matrix.
Development (Cambridge, England)145(22)(2018 11)
Love AM,Prince DJ,Jessen JR

Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages in zebrafish spinal cord regeneration.
Nature communications9(1)4670(2018 11)
Tsarouchas TM,Wehner D,Cavone L,Munir T,Keatinge M,Lambertus M,Underhill A,Barrett T,Kassapis E,Ogryzko N,Feng Y,van Ham TJ,Becker T,Becker CG

Glypican 4 and Mmp14 interact in regulating the migration of anterior endodermal cells by limiting extracellular matrix deposition.
Development (Cambridge, England)145(17)(2018 08)
Hu B,Gao Y,Davies L,Woo S,Topczewski J,Jessen JR,Lin F

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fn1b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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