arhgef10 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef10
Entrez Gene ID	334278
Full Name	Rho guanine nucleotide exchange factor (GEF) 10
Synonyms	wu:fi38h08
General protein information	Preferred Names Rho guanine nucleotide exchange factor (GEF) 10 Names rho guanine nucleotide exchange factor 10
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293079.3	XP_009291354.2	rho guanine nucleotide exchange factor 10 isoform X1
XM_009293082.2	XP_009291357.1	rho guanine nucleotide exchange factor 10 isoform X3
XM_021473177.1	XP_021328852.1	rho guanine nucleotide exchange factor 10 isoform X2
XM_021467421.1	XP_021323096.1	rho guanine nucleotide exchange factor 10 isoform X1
XM_009293081.2	XP_009291356.1	rho guanine nucleotide exchange factor 10 isoform X2
XM_009293081.3	XP_009291356.1	rho guanine nucleotide exchange factor 10 isoform X2
XM_009293079.2	XP_009291354.1	rho guanine nucleotide exchange factor 10 isoform X1
XM_021473176.1	XP_021328851.1	rho guanine nucleotide exchange factor 10 isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Arhgef10	XP_006253464.1
Gallus gallus (chicken)	ARHGEF10	XP_001235643.3
Macaca mulatta (Rhesus monkey)	LOC722745	XP_002805294.1
Homo sapiens (human)	ARHGEF10	NP_055444.2
Canis lupus familiaris (dog)	ARHGEF10	XP_005630094.1
Mus musculus (house mouse)	Arhgef10	NP_766339.2
Xenopus tropicalis (tropical clawed frog)	arhgef10	XP_002935203.2
Danio rerio (zebrafish)	arhgef10	XP_005158599.1
Bos taurus (cattle)	ARHGEF10	XP_005225971.1

Gene Ontology

The following arhgef10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145918	XM_009293079.3 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa145919	XM_021473176.1 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa145920	XM_021473177.1 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa39976	XM_009293079.2 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa39976	XM_021467421.1 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa39978	XM_009293081.3 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa39979	XM_009293082.2 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa39978	XM_009293081.2	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef10 ( XM_009293079.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291354.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa145918
Clone ID Related Accession (Same CDS sequence)	XM_009293079.3
Accession Version	XM_009293079.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394991.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA 
GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG 
CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA 
ACCAAAGCCG CAGTGAGAAA GGGCCGATCG TTCATCCGCA CCAGATCTCT CTGTCATGAG 
AAGAAACCCA GCTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT 
GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA 
TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT 
TTAGAGCAGT ATGAGAAGCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG 
CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG 
GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA 
GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC 
TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC 
AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT 
ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG 
GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA 
AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA 
GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG 
TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA 
CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT 
CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CTTCAGGACA GCGTTTCTTG 
CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG 
ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA 
CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT 
GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG 
AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG 
AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA 
CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC 
ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC 
ATCCAAGGCT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGGAAGCA GAATCATCCT 
CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT 
GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG 
GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG 
GCACTGCAAA ACTCAAATCC TAAAGTGACA GAGTGTTTTA ATGTCGAGTC GCGTATTCTC 
TGTATGACGC ATGTACCGTT GGATGAGCCA CTAAAGAACG TTGAGGGAAA ACCTGAGAAT 
CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT 
TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG 
TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTCAGTGGT 
TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG 
TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGGGCGAACA CATCTGGGCT 
TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT 
GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC 
TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC 
CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC 
TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC 
ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG 
GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT 
AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT 
GAGGCCTCAG AGCTGGAGGG AGCCACACTA AACCAGAACT GCAGCAGGGG CTCACATGCG 
CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT 
TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC 
CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG 
CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC 
ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291354.2
CDS	202..4440
Translation	MFSMDVEDFPPPPPDLLDDEDEGERFEFDDSGDEAPEADRPPPEPEGISVPPPCSSSVRDAPVNEASPNPEHTFNAEADLPPPPQDFPANNRPLAEALFKQSDLPPLPPPPPTELCPGAAEGRKIDLNPPAEGTDVHRMQDENKGASVVCSSKPKVSPYSVIDIGPIQQQLPSEQSGPPSSPSANQIEGVVQEVSGSPGLSSGYSVPVPCGYATPSNVPVIPPTYTTPVLIRHFSMDEDVTVVGAVNTPVDSVFSEECPVIKEEDALTKWVSDPANTAWMESPDEVIYDDVPRENSGSTTDPDEMIYDDVEFGEEGGSSSLDNGWSSSEFESYEENSDVENGHGENGLPDAFVRGRAPSKKTHLSEDLARLKEHYERKMRDLMANTVGTVELQQLKHKHEQKMQRLVKAAKDGTKDGLQKTKAAVRKGRSFIRTRSLCHEKKPSCFDEESDLFIEVECFNVDPVLGPAPDGLTQHQVMRRCILEKILESEKSYLEALKRILEQYEKPLAEIEPRLLSDRKRKVVFHRIREILQCHALFQMALASRVSDWDELEMIGDVFVASFSKSMVLDAYSEYVNNFSLAMGVVRKACAAKPCFLDFLKHRQETSKDRLTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHADRLPLQMALTELEVLAEKLNERKKEADQRCEIRHIAKAMNEHYLNKLLSSGSRYLIRSDDMVETVYNDRGEIIKTKERRVFLLNDVLMCATPNRRPSQDGVSVGAGGEGVPSGQRFLLKWSVPLSFVEVVEFGSSEEMVDSRFPPSHSGDKVVINAKPNKLYMGPGQLYQDLQNLIHDLGLVNQISTMISSLKGNYQNVNGTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKYGRPTFFTAVFNTFSPSIKQTWISNLQMAKLALQEDNIQGWFCAEDDGNQMRKQNHPLLLKQMAVVMSKLQEFKVECAVHNPEPHVNTDSTADLPVMGHGCVWVASCTNHMGQVSIVALQNSNPKVTECFNVESRILCMTHVPLDEPLKNVEGKPENRNACDMPTVCLGMEEGSISVYKSSQRSKKVRLQHFFSPDKSSVTCLVYTSGCLYAGLVSGSLVIYSRTDDGFWVESGACVLKLGVLPVKALLVVGEHIWASSGGQIFIISTQTNTVERQFEAHQEEGMIVSHMVVAGVGIWISFSTGSTLRLFHTETLDHLQDINIATAVNNILPGQQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSFHAHHGPVKFLTMATAVCNVSQATMATLTSSTPSQCQPEASELEGATLNQNCSRGSHAPVLQDSPSSSCGSLALSQASLEHGPEDGAIYDLLNEPAHFQKAKQTDRVSASSVLVVSGGLGHRRINRKTKQSKHEEMASTIMVWQIPLPSL

Target ORF information:

RefSeq Version	XM_009293079.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293079.3

ORF Insert Sequence:

ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA 
GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG 
CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA 
ACCAAAGCCG CAGTGAGAAA GGGCCGATCG TTCATCCGCA CCAGATCTCT CTGTCATGAG 
AAGAAACCCA GCTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT 
GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA 
TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT 
TTAGAGCAGT ATGAGAAGCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG 
CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG 
GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA 
GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC 
TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC 
AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT 
ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG 
GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA 
AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA 
GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG 
TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA 
CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT 
CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CTTCAGGACA GCGTTTCTTG 
CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG 
ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA 
CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT 
GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG 
AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG 
AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA 
CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC 
ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC 
ATCCAAGGCT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGGAAGCA GAATCATCCT 
CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT 
GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG 
GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG 
GCACTGCAAA ACTCAAATCC TAAAGTGACA GAGTGTTTTA ATGTCGAGTC GCGTATTCTC 
TGTATGACGC ATGTACCGTT GGATGAGCCA CTAAAGAACG TTGAGGGAAA ACCTGAGAAT 
CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT 
TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG 
TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTCAGTGGT 
TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG 
TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGGGCGAACA CATCTGGGCT 
TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT 
GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC 
TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC 
CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC 
TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC 
ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG 
GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT 
AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT 
GAGGCCTCAG AGCTGGAGGG AGCCACACTA AACCAGAACT GCAGCAGGGG CTCACATGCG 
CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT 
TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC 
CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG 
CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC 
ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef10 ( XM_021473176.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328851.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa145919
Clone ID Related Accession (Same CDS sequence)	XM_021473176.1
Accession Version	XM_021473176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394991.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA 
GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG 
CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA 
ACCAAAGCCG CAGTGAGAAA GGGCCGATCG TTCATCCGCA CCAGATCTCT CTGTCATGAG 
AAGAAACCCA GCTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT 
GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA 
TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT 
TTAGAGTATG AGAAGCCTCT GGCAGAAATT GAGCCTCGGT TACTCAGTGA CCGCAAGCGT 
AAGGTGGTGT TTCATCGCAT TCGGGAGATC CTGCAGTGTC ATGCACTCTT CCAGATGGCG 
CTTGCCAGCA GAGTATCTGA CTGGGATGAG CTGGAGATGA TTGGAGATGT GTTTGTAGCA 
TCGTTTTCCA AGTCTATGGT GTTGGACGCT TACAGTGAAT ATGTGAATAA CTTCAGCTTG 
GCGATGGGAG TGGTGCGGAA GGCATGCGCC GCTAAACCCT GTTTCCTGGA CTTCCTCAAG 
CACCGCCAGG AGACAAGTAA AGACCGTCTG ACTCTTTATG GACTGATGAT GAAGCCTATT 
CAGCGGTTCC CTCAGTTTAT TCTGCTGCTG CAAGACATGT TAAAGAACAC GCCAGTGGGC 
CATGCTGATC GCCTTCCCCT TCAGATGGCT CTTACTGAAC TGGAAGTGCT TGCCGAAAAA 
TTAAACGAAA GGAAAAAGGA GGCTGACCAG CGCTGCGAGA TACGACACAT CGCTAAAGCT 
ATGAACGAAC ATTACCTCAA CAAGCTGCTG AGCAGCGGCA GCCGTTATCT GATTCGGTCT 
GATGACATGG TGGAAACCGT TTATAATGAT CGAGGGGAGA TCATTAAGAC TAAAGAACGG 
CGAGTCTTTC TCCTGAACGA TGTGCTCATG TGTGCCACCC CCAACCGGCG GCCATCTCAG 
GATGGTGTGA GTGTTGGAGC TGGAGGTGAA GGGGTTCCTT CAGGACAGCG TTTCTTGCTC 
AAGTGGAGTG TACCGTTAAG TTTTGTGGAG GTCGTGGAGT TTGGCTCCAG CGAGGAGATG 
GTTGACTCCC GGTTTCCACC CTCACACTCT GGAGATAAAG TTGTCATTAA TGCAAAACCT 
AATAAGTTAT ATATGGGGCC GGGACAGCTG TATCAGGACC TGCAGAACCT GATCCATGAC 
CTGGGCTTGG TGAACCAAAT TTCCACCATG ATCAGCAGTC TGAAAGGGAA TTATCAGAAT 
GTTAATGGGA CTGTTGCTCA GGACTGGGTT TCAGGTCTTC AGCGTCTCAT TTTAAAGAAA 
GAGGAGGAGA TTCGGGCAGC TGATCGCTGT AGGATACAAC TACAGTTACC AGGGAAACAA 
GACAAATACG GTCGACCCAC GTTTTTCACT GCAGTCTTCA ACACATTCAG TCCATCCATC 
AAGCAAACAT GGATCAGTAA TCTGCAGATG GCTAAACTGG CTTTACAGGA GGACAACATC 
CAAGGCTGGT TTTGTGCTGA AGATGATGGT AATCAGATGA GGAAGCAGAA TCATCCTCTC 
TTGCTCAAGC AAATGGCAGT GGTCATGTCT AAACTACAGG AGTTTAAGGT GGAGTGTGCC 
GTTCACAACC CCGAGCCTCA TGTGAACACC GACAGCACCG CAGATCTGCC TGTTATGGGT 
CACGGATGTG TGTGGGTGGC GAGTTGCACC AATCACATGG GTCAGGTGTC CATTGTGGCA 
CTGCAAAACT CAAATCCTAA AGTGACAGAG TGTTTTAATG TCGAGTCGCG TATTCTCTGT 
ATGACGCATG TACCGTTGGA TGAGCCACTA AAGAACGTTG AGGGAAAACC TGAGAATCGG 
AATGCCTGTG ATATGCCAAC TGTCTGTTTG GGAATGGAGG AAGGCAGTAT ATCTGTTTAT 
AAGAGCAGCC AGCGCTCTAA GAAAGTTCGC CTGCAGCACT TCTTCTCTCC TGATAAGTCT 
AGCGTCACGT GTTTGGTGTA CACGAGTGGG TGTCTGTATG CAGGTCTAGT CAGTGGTTCT 
CTGGTCATCT ACTCCAGAAC TGATGACGGC TTCTGGGTCG AGTCGGGGGC CTGTGTGTTG 
AAGCTGGGGG TGTTGCCGGT TAAAGCTCTA CTGGTGGTGG GCGAACACAT CTGGGCTTCA 
TCAGGAGGAC AGATCTTCAT CATCAGCACA CAGACAAACA CGGTGGAGCG TCAGTTTGAA 
GCCCATCAGG AAGAAGGAAT GATCGTGTCC CACATGGTGG TGGCTGGAGT GGGTATCTGG 
ATATCCTTCA GCACCGGCTC AACCCTTCGA CTCTTCCACA CCGAGACTCT CGACCACCTG 
CAAGACATCA ACATCGCCAC AGCTGTCAAT AACATACTCC CAGGACAGCA GAGAGTCTCT 
GTGAGCAGTC TGTTGGTGTG TCACGGTCTG CTGCTAGTGG GCACTAATCT GGGGGTCACG 
GTGGCCCTTT CTGTGCCCAG ACTACAGGGC ATCCCAAAGG TCACAGGTCG AGGAATGGTC 
TCTTTTCATG CACACCACGG CCCAGTCAAG TTCCTCACCA TGGCAACAGC AGTGTGTAAC 
GTCTCTCAAG CTACCATGGC AACCCTCACC TCCAGTACTC CCAGTCAGTG CCAGCCTGAG 
GCCTCAGAGC TGGAGGGAGC CACACTAAAC CAGAACTGCA GCAGGGGCTC ACATGCGCCT 
GTTCTCCAGG ACTCCCCGTC CTCCTCGTGT GGCTCTCTGG CTCTGTCTCA GGCATCTTTA 
GAGCACGGCC CAGAGGACGG GGCCATATAC GACCTGCTGA ACGAGCCGGC ACACTTCCAG 
AAGGCCAAGC AGACTGACAG AGTCAGTGCC AGCTCTGTGC TGGTGGTGTC AGGAGGGCTC 
GGACACCGCA GAATCAACCG AAAAACCAAA CAGTCCAAGC ACGAGGAGAT GGCGTCCACT 
ATCATGGTGT GGCAAATACC ACTGCCAAGC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328851.1
CDS	202..4437
Translation	MFSMDVEDFPPPPPDLLDDEDEGERFEFDDSGDEAPEADRPPPEPEGISVPPPCSSSVRDAPVNEASPNPEHTFNAEADLPPPPQDFPANNRPLAEALFKQSDLPPLPPPPPTELCPGAAEGRKIDLNPPAEGTDVHRMQDENKGASVVCSSKPKVSPYSVIDIGPIQQQLPSEQSGPPSSPSANQIEGVVQEVSGSPGLSSGYSVPVPCGYATPSNVPVIPPTYTTPVLIRHFSMDEDVTVVGAVNTPVDSVFSEECPVIKEEDALTKWVSDPANTAWMESPDEVIYDDVPRENSGSTTDPDEMIYDDVEFGEEGGSSSLDNGWSSSEFESYEENSDVENGHGENGLPDAFVRGRAPSKKTHLSEDLARLKEHYERKMRDLMANTVGTVELQQLKHKHEQKMQRLVKAAKDGTKDGLQKTKAAVRKGRSFIRTRSLCHEKKPSCFDEESDLFIEVECFNVDPVLGPAPDGLTQHQVMRRCILEKILESEKSYLEALKRILEYEKPLAEIEPRLLSDRKRKVVFHRIREILQCHALFQMALASRVSDWDELEMIGDVFVASFSKSMVLDAYSEYVNNFSLAMGVVRKACAAKPCFLDFLKHRQETSKDRLTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHADRLPLQMALTELEVLAEKLNERKKEADQRCEIRHIAKAMNEHYLNKLLSSGSRYLIRSDDMVETVYNDRGEIIKTKERRVFLLNDVLMCATPNRRPSQDGVSVGAGGEGVPSGQRFLLKWSVPLSFVEVVEFGSSEEMVDSRFPPSHSGDKVVINAKPNKLYMGPGQLYQDLQNLIHDLGLVNQISTMISSLKGNYQNVNGTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKYGRPTFFTAVFNTFSPSIKQTWISNLQMAKLALQEDNIQGWFCAEDDGNQMRKQNHPLLLKQMAVVMSKLQEFKVECAVHNPEPHVNTDSTADLPVMGHGCVWVASCTNHMGQVSIVALQNSNPKVTECFNVESRILCMTHVPLDEPLKNVEGKPENRNACDMPTVCLGMEEGSISVYKSSQRSKKVRLQHFFSPDKSSVTCLVYTSGCLYAGLVSGSLVIYSRTDDGFWVESGACVLKLGVLPVKALLVVGEHIWASSGGQIFIISTQTNTVERQFEAHQEEGMIVSHMVVAGVGIWISFSTGSTLRLFHTETLDHLQDINIATAVNNILPGQQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSFHAHHGPVKFLTMATAVCNVSQATMATLTSSTPSQCQPEASELEGATLNQNCSRGSHAPVLQDSPSSSCGSLALSQASLEHGPEDGAIYDLLNEPAHFQKAKQTDRVSASSVLVVSGGLGHRRINRKTKQSKHEEMASTIMVWQIPLPSL

Target ORF information:

RefSeq Version	XM_021473176.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473176.1

ORF Insert Sequence:

ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA 
GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG 
CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA 
ACCAAAGCCG CAGTGAGAAA GGGCCGATCG TTCATCCGCA CCAGATCTCT CTGTCATGAG 
AAGAAACCCA GCTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT 
GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA 
TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT 
TTAGAGTATG AGAAGCCTCT GGCAGAAATT GAGCCTCGGT TACTCAGTGA CCGCAAGCGT 
AAGGTGGTGT TTCATCGCAT TCGGGAGATC CTGCAGTGTC ATGCACTCTT CCAGATGGCG 
CTTGCCAGCA GAGTATCTGA CTGGGATGAG CTGGAGATGA TTGGAGATGT GTTTGTAGCA 
TCGTTTTCCA AGTCTATGGT GTTGGACGCT TACAGTGAAT ATGTGAATAA CTTCAGCTTG 
GCGATGGGAG TGGTGCGGAA GGCATGCGCC GCTAAACCCT GTTTCCTGGA CTTCCTCAAG 
CACCGCCAGG AGACAAGTAA AGACCGTCTG ACTCTTTATG GACTGATGAT GAAGCCTATT 
CAGCGGTTCC CTCAGTTTAT TCTGCTGCTG CAAGACATGT TAAAGAACAC GCCAGTGGGC 
CATGCTGATC GCCTTCCCCT TCAGATGGCT CTTACTGAAC TGGAAGTGCT TGCCGAAAAA 
TTAAACGAAA GGAAAAAGGA GGCTGACCAG CGCTGCGAGA TACGACACAT CGCTAAAGCT 
ATGAACGAAC ATTACCTCAA CAAGCTGCTG AGCAGCGGCA GCCGTTATCT GATTCGGTCT 
GATGACATGG TGGAAACCGT TTATAATGAT CGAGGGGAGA TCATTAAGAC TAAAGAACGG 
CGAGTCTTTC TCCTGAACGA TGTGCTCATG TGTGCCACCC CCAACCGGCG GCCATCTCAG 
GATGGTGTGA GTGTTGGAGC TGGAGGTGAA GGGGTTCCTT CAGGACAGCG TTTCTTGCTC 
AAGTGGAGTG TACCGTTAAG TTTTGTGGAG GTCGTGGAGT TTGGCTCCAG CGAGGAGATG 
GTTGACTCCC GGTTTCCACC CTCACACTCT GGAGATAAAG TTGTCATTAA TGCAAAACCT 
AATAAGTTAT ATATGGGGCC GGGACAGCTG TATCAGGACC TGCAGAACCT GATCCATGAC 
CTGGGCTTGG TGAACCAAAT TTCCACCATG ATCAGCAGTC TGAAAGGGAA TTATCAGAAT 
GTTAATGGGA CTGTTGCTCA GGACTGGGTT TCAGGTCTTC AGCGTCTCAT TTTAAAGAAA 
GAGGAGGAGA TTCGGGCAGC TGATCGCTGT AGGATACAAC TACAGTTACC AGGGAAACAA 
GACAAATACG GTCGACCCAC GTTTTTCACT GCAGTCTTCA ACACATTCAG TCCATCCATC 
AAGCAAACAT GGATCAGTAA TCTGCAGATG GCTAAACTGG CTTTACAGGA GGACAACATC 
CAAGGCTGGT TTTGTGCTGA AGATGATGGT AATCAGATGA GGAAGCAGAA TCATCCTCTC 
TTGCTCAAGC AAATGGCAGT GGTCATGTCT AAACTACAGG AGTTTAAGGT GGAGTGTGCC 
GTTCACAACC CCGAGCCTCA TGTGAACACC GACAGCACCG CAGATCTGCC TGTTATGGGT 
CACGGATGTG TGTGGGTGGC GAGTTGCACC AATCACATGG GTCAGGTGTC CATTGTGGCA 
CTGCAAAACT CAAATCCTAA AGTGACAGAG TGTTTTAATG TCGAGTCGCG TATTCTCTGT 
ATGACGCATG TACCGTTGGA TGAGCCACTA AAGAACGTTG AGGGAAAACC TGAGAATCGG 
AATGCCTGTG ATATGCCAAC TGTCTGTTTG GGAATGGAGG AAGGCAGTAT ATCTGTTTAT 
AAGAGCAGCC AGCGCTCTAA GAAAGTTCGC CTGCAGCACT TCTTCTCTCC TGATAAGTCT 
AGCGTCACGT GTTTGGTGTA CACGAGTGGG TGTCTGTATG CAGGTCTAGT CAGTGGTTCT 
CTGGTCATCT ACTCCAGAAC TGATGACGGC TTCTGGGTCG AGTCGGGGGC CTGTGTGTTG 
AAGCTGGGGG TGTTGCCGGT TAAAGCTCTA CTGGTGGTGG GCGAACACAT CTGGGCTTCA 
TCAGGAGGAC AGATCTTCAT CATCAGCACA CAGACAAACA CGGTGGAGCG TCAGTTTGAA 
GCCCATCAGG AAGAAGGAAT GATCGTGTCC CACATGGTGG TGGCTGGAGT GGGTATCTGG 
ATATCCTTCA GCACCGGCTC AACCCTTCGA CTCTTCCACA CCGAGACTCT CGACCACCTG 
CAAGACATCA ACATCGCCAC AGCTGTCAAT AACATACTCC CAGGACAGCA GAGAGTCTCT 
GTGAGCAGTC TGTTGGTGTG TCACGGTCTG CTGCTAGTGG GCACTAATCT GGGGGTCACG 
GTGGCCCTTT CTGTGCCCAG ACTACAGGGC ATCCCAAAGG TCACAGGTCG AGGAATGGTC 
TCTTTTCATG CACACCACGG CCCAGTCAAG TTCCTCACCA TGGCAACAGC AGTGTGTAAC 
GTCTCTCAAG CTACCATGGC AACCCTCACC TCCAGTACTC CCAGTCAGTG CCAGCCTGAG 
GCCTCAGAGC TGGAGGGAGC CACACTAAAC CAGAACTGCA GCAGGGGCTC ACATGCGCCT 
GTTCTCCAGG ACTCCCCGTC CTCCTCGTGT GGCTCTCTGG CTCTGTCTCA GGCATCTTTA 
GAGCACGGCC CAGAGGACGG GGCCATATAC GACCTGCTGA ACGAGCCGGC ACACTTCCAG 
AAGGCCAAGC AGACTGACAG AGTCAGTGCC AGCTCTGTGC TGGTGGTGTC AGGAGGGCTC 
GGACACCGCA GAATCAACCG AAAAACCAAA CAGTCCAAGC ACGAGGAGAT GGCGTCCACT 
ATCATGGTGT GGCAAATACC ACTGCCAAGC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef10 ( XM_021473177.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328852.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

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CloneID	ODa145920
Clone ID Related Accession (Same CDS sequence)	XM_021473177.1
Accession Version	XM_021473177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394991.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG 
AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCGTTCATCC GCACCAGATC TCTCTGTCAT 
GAGAAGAAAC CCAGCTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT 
AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG 
AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA 
ATTTTAGAGC AGTATGAGAA GCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC 
AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG 
ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT 
GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC 
AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC 
CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG 
CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA 
GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC 
GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT 
AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT 
CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA 
GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA 
TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCTTCAGG ACAGCGTTTC 
TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG 
GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA 
AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC 
CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT 
CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA 
AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG 
AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA 
TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC 
AACATCCAAG GCTGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGGAA GCAGAATCAT 
CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG 
TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT 
ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT 
GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACAGAGTGTT TTAATGTCGA GTCGCGTATT 
CTCTGTATGA CGCATGTACC GTTGGATGAG CCACTAAAGA ACGTTGAGGG AAAACCTGAG 
AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT 
GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT 
AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTCAGT 
GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT 
GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGGGCGA ACACATCTGG 
GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG 
TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT 
ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC 
CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA 
GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG 
GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA 
ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG 
TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG 
CCTGAGGCCT CAGAGCTGGA GGGAGCCACA CTAAACCAGA ACTGCAGCAG GGGCTCACAT 
GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA 
TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC 
TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA 
GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG 
TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328852.1
CDS	202..4323
Translation	MFSMDVEDFPPPPPDLLDDEDEGERFEFDDSGDEAPEADRPPPEPEGISVPPPCSSSVRDAPVNEASPNPEHTFNAEADLPPPPQDFPANNRPLAEALFKQSDLPPLPPPPPTELCPGAAEGRKIDLNPPAEGTDVHRMQDENKGASVVCSSKPKVSPYSVIDIGPIQQQLPSEQSGPPSSPSANQIEGVVQEVSGSPGLSSGYSVPVPCGYATPSNVPVIPPTYTTPVLIRHFSMDEDVTVVGAVNTPVDSVFSEECPVIKEEDALTKWVSDPANTAWMESPDEVIYDDVPRENSGSTTDPDEMIYDDVEFGEEGGSSSLDNGWSSSEFESYEENSDVENGHGENGLPDAFVRGRAPSKKTHMQRLVKAAKDGTKDGLQKTKAAVRKGRSFIRTRSLCHEKKPSCFDEESDLFIEVECFNVDPVLGPAPDGLTQHQVMRRCILEKILESEKSYLEALKRILEQYEKPLAEIEPRLLSDRKRKVVFHRIREILQCHALFQMALASRVSDWDELEMIGDVFVASFSKSMVLDAYSEYVNNFSLAMGVVRKACAAKPCFLDFLKHRQETSKDRLTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHADRLPLQMALTELEVLAEKLNERKKEADQRCEIRHIAKAMNEHYLNKLLSSGSRYLIRSDDMVETVYNDRGEIIKTKERRVFLLNDVLMCATPNRRPSQDGVSVGAGGEGVPSGQRFLLKWSVPLSFVEVVEFGSSEEMVDSRFPPSHSGDKVVINAKPNKLYMGPGQLYQDLQNLIHDLGLVNQISTMISSLKGNYQNVNGTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKYGRPTFFTAVFNTFSPSIKQTWISNLQMAKLALQEDNIQGWFCAEDDGNQMRKQNHPLLLKQMAVVMSKLQEFKVECAVHNPEPHVNTDSTADLPVMGHGCVWVASCTNHMGQVSIVALQNSNPKVTECFNVESRILCMTHVPLDEPLKNVEGKPENRNACDMPTVCLGMEEGSISVYKSSQRSKKVRLQHFFSPDKSSVTCLVYTSGCLYAGLVSGSLVIYSRTDDGFWVESGACVLKLGVLPVKALLVVGEHIWASSGGQIFIISTQTNTVERQFEAHQEEGMIVSHMVVAGVGIWISFSTGSTLRLFHTETLDHLQDINIATAVNNILPGQQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSFHAHHGPVKFLTMATAVCNVSQATMATLTSSTPSQCQPEASELEGATLNQNCSRGSHAPVLQDSPSSSCGSLALSQASLEHGPEDGAIYDLLNEPAHFQKAKQTDRVSASSVLVVSGGLGHRRINRKTKQSKHEEMASTIMVWQIPLPSL

Target ORF information:

RefSeq Version	XM_021473177.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473177.1

ORF Insert Sequence:

ATGTTCAGCA TGGACGTGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCAGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TAGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCCGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAATGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCTG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGAGG TGTCCGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC GAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG 
AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCGTTCATCC GCACCAGATC TCTCTGTCAT 
GAGAAGAAAC CCAGCTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT 
AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG 
AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA 
ATTTTAGAGC AGTATGAGAA GCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC 
AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG 
ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT 
GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC 
AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC 
CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG 
CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA 
GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC 
GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT 
AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT 
CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA 
GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA 
TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCTTCAGG ACAGCGTTTC 
TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG 
GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA 
AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC 
CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT 
CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA 
AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG 
AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA 
TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC 
AACATCCAAG GCTGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGGAA GCAGAATCAT 
CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG 
TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT 
ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT 
GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACAGAGTGTT TTAATGTCGA GTCGCGTATT 
CTCTGTATGA CGCATGTACC GTTGGATGAG CCACTAAAGA ACGTTGAGGG AAAACCTGAG 
AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT 
GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT 
AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTCAGT 
GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT 
GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGGGCGA ACACATCTGG 
GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG 
TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT 
ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC 
CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA 
GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG 
GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA 
ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG 
TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG 
CCTGAGGCCT CAGAGCTGGA GGGAGCCACA CTAAACCAGA ACTGCAGCAG GGGCTCACAT 
GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA 
TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC 
TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA 
GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG 
TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef10 ( XM_021467421.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323096.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

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CloneID	ODa39976
Clone ID Related Accession (Same CDS sequence)	XM_021467421.1 , XM_009293079.2
Accession Version	XM_021467421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA 
GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG 
CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA 
ACCAAAGCCG CAGTGAGAAA GGGCCGATCA TTCATCCGCA CCAGATCTCT CTGTCATGAT 
AAGAAACCCA ACTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT 
GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA 
TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT 
TTAGAGCAGT ATGAGAATCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG 
CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG 
GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA 
GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC 
TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC 
AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT 
ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG 
GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA 
AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA 
GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG 
TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA 
CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT 
CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CCTCAGGACA GCGTTTCTTG 
CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG 
ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA 
CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT 
GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG 
AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG 
AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA 
CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC 
ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC 
ATCCAAGGAT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGAAAGCA GAATCATCCT 
CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT 
GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG 
GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG 
GCACTGCAAA ACTCAAATCC TAAAGTGACG GAGTGTTTTA ATGTCGAGTC GCGTATTCTC 
TGTATGACGC ATGTTCCGTT GGATGAGCCA CTAAAGAACG GTGAGGGAAA ACCTGAGAAT 
CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT 
TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG 
TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTTAGTGGT 
TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG 
TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGAGCGAACA CATCTGGGCT 
TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT 
GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC 
TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC 
CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC 
TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC 
ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG 
GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT 
AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT 
GAGGCCTCGG AACTGGAGGT AGCCACACTA AACCAGAACT GCAGCAGCGG CTCACATGCG 
CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT 
TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC 
CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG 
CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC 
ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323096.1
CDS	204..4442
Translation	MFSMDMEDFPPPPPDLLDDEDEGERFEFDDSGDEAPEADRPPPEPEGISVPPPCSSSVRDAPVNEASPNPEHTFNAEADLPPPPQDFPANNRPLAEALFKQSDLPPLPPPPPTELCPGAAEGWKIDLNPPAEGTDVHREQDENKGASVVCSSKPKVSPYSVIDIGPIQQQLPSEQSGPPSSPSANQIEGVVQDVLGSPGLSSGYSVPVPCGYATPSNVPVIPPTYTTPVLIRHFSMDEDVTVVGAVNTPVDSVFSEECPVIKEEDALTKWVSDPANTAWMESPDEVIYDDVPRENSGSTTDPDEMIYDDVEFGEEGGSSSLDNGWSSSEFESYEENSDVENGHGENGLPDAFVRGRAPSKKTHLSEDLARLKEHYERKMRDLMANTVGTVELQQLKHKHEQKMQRLVKAAKDGTKDGLQKTKAAVRKGRSFIRTRSLCHDKKPNCFDEESDLFIEVECFNVDPVLGPAPDGLTQHQVMRRCILEKILESEKSYLEALKRILEQYENPLAEIEPRLLSDRKRKVVFHRIREILQCHALFQMALASRVSDWDELEMIGDVFVASFSKSMVLDAYSEYVNNFSLAMGVVRKACAAKPCFLDFLKHRQETSKDRLTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHADRLPLQMALTELEVLAEKLNERKKEADQRCEIRHIAKAMNEHYLNKLLSSGSRYLIRSDDMVETVYNDRGEIIKTKERRVFLLNDVLMCATPNRRPSQDGVSVGAGGEGVPSGQRFLLKWSVPLSFVEVVEFGSSEEMVDSRFPPSHSGDKVVINAKPNKLYMGPGQLYQDLQNLIHDLGLVNQISTMISSLKGNYQNVNGTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKYGRPTFFTAVFNTFSPSIKQTWISNLQMAKLALQEDNIQGWFCAEDDGNQMRKQNHPLLLKQMAVVMSKLQEFKVECAVHNPEPHVNTDSTADLPVMGHGCVWVASCTNHMGQVSIVALQNSNPKVTECFNVESRILCMTHVPLDEPLKNGEGKPENRNACDMPTVCLGMEEGSISVYKSSQRSKKVRLQHFFSPDKSSVTCLVYTSGCLYAGLVSGSLVIYSRTDDGFWVESGACVLKLGVLPVKALLVVSEHIWASSGGQIFIISTQTNTVERQFEAHQEEGMIVSHMVVAGVGIWISFSTGSTLRLFHTETLDHLQDINIATAVNNILPGQQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSFHAHHGPVKFLTMATAVCNVSQATMATLTSSTPSQCQPEASELEVATLNQNCSSGSHAPVLQDSPSSSCGSLALSQASLEHGPEDGAIYDLLNEPAHFQKAKQTDRVSASSVLVVSGGLGHRRINRKTKQSKHEEMASTIMVWQIPLPSL

Target ORF information:

RefSeq Version	XM_021467421.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467421.1

ORF Insert Sequence:

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA 
GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG 
CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA 
ACCAAAGCCG CAGTGAGAAA GGGCCGATCA TTCATCCGCA CCAGATCTCT CTGTCATGAT 
AAGAAACCCA ACTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT 
GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA 
TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT 
TTAGAGCAGT ATGAGAATCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG 
CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG 
GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA 
GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC 
TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC 
AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT 
ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG 
GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA 
AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA 
GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG 
TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA 
CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT 
CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CCTCAGGACA GCGTTTCTTG 
CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG 
ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA 
CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT 
GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG 
AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG 
AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA 
CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC 
ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC 
ATCCAAGGAT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGAAAGCA GAATCATCCT 
CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT 
GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG 
GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG 
GCACTGCAAA ACTCAAATCC TAAAGTGACG GAGTGTTTTA ATGTCGAGTC GCGTATTCTC 
TGTATGACGC ATGTTCCGTT GGATGAGCCA CTAAAGAACG GTGAGGGAAA ACCTGAGAAT 
CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT 
TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG 
TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTTAGTGGT 
TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG 
TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGAGCGAACA CATCTGGGCT 
TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT 
GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC 
TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC 
CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC 
TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC 
ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG 
GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT 
AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT 
GAGGCCTCGG AACTGGAGGT AGCCACACTA AACCAGAACT GCAGCAGCGG CTCACATGCG 
CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT 
TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC 
CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG 
CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC 
ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef10 ( XM_009293079.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291354.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

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CloneID	ODa39976
Clone ID Related Accession (Same CDS sequence)	XM_021467421.1 , XM_009293079.2
Accession Version	XM_009293079.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rho guanine nucleotide exchange factor 10 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA 
GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG 
CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA 
ACCAAAGCCG CAGTGAGAAA GGGCCGATCA TTCATCCGCA CCAGATCTCT CTGTCATGAT 
AAGAAACCCA ACTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT 
GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA 
TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT 
TTAGAGCAGT ATGAGAATCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG 
CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG 
GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA 
GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC 
TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC 
AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT 
ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG 
GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA 
AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA 
GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG 
TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA 
CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT 
CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CCTCAGGACA GCGTTTCTTG 
CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG 
ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA 
CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT 
GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG 
AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG 
AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA 
CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC 
ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC 
ATCCAAGGAT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGAAAGCA GAATCATCCT 
CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT 
GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG 
GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG 
GCACTGCAAA ACTCAAATCC TAAAGTGACG GAGTGTTTTA ATGTCGAGTC GCGTATTCTC 
TGTATGACGC ATGTTCCGTT GGATGAGCCA CTAAAGAACG GTGAGGGAAA ACCTGAGAAT 
CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT 
TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG 
TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTTAGTGGT 
TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG 
TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGAGCGAACA CATCTGGGCT 
TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT 
GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC 
TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC 
CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC 
TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC 
ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG 
GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT 
AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT 
GAGGCCTCGG AACTGGAGGT AGCCACACTA AACCAGAACT GCAGCAGCGG CTCACATGCG 
CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT 
TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC 
CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG 
CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC 
ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291354.1
CDS	200..4438
Translation	MFSMDMEDFPPPPPDLLDDEDEGERFEFDDSGDEAPEADRPPPEPEGISVPPPCSSSVRDAPVNEASPNPEHTFNAEADLPPPPQDFPANNRPLAEALFKQSDLPPLPPPPPTELCPGAAEGWKIDLNPPAEGTDVHREQDENKGASVVCSSKPKVSPYSVIDIGPIQQQLPSEQSGPPSSPSANQIEGVVQDVLGSPGLSSGYSVPVPCGYATPSNVPVIPPTYTTPVLIRHFSMDEDVTVVGAVNTPVDSVFSEECPVIKEEDALTKWVSDPANTAWMESPDEVIYDDVPRENSGSTTDPDEMIYDDVEFGEEGGSSSLDNGWSSSEFESYEENSDVENGHGENGLPDAFVRGRAPSKKTHLSEDLARLKEHYERKMRDLMANTVGTVELQQLKHKHEQKMQRLVKAAKDGTKDGLQKTKAAVRKGRSFIRTRSLCHDKKPNCFDEESDLFIEVECFNVDPVLGPAPDGLTQHQVMRRCILEKILESEKSYLEALKRILEQYENPLAEIEPRLLSDRKRKVVFHRIREILQCHALFQMALASRVSDWDELEMIGDVFVASFSKSMVLDAYSEYVNNFSLAMGVVRKACAAKPCFLDFLKHRQETSKDRLTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHADRLPLQMALTELEVLAEKLNERKKEADQRCEIRHIAKAMNEHYLNKLLSSGSRYLIRSDDMVETVYNDRGEIIKTKERRVFLLNDVLMCATPNRRPSQDGVSVGAGGEGVPSGQRFLLKWSVPLSFVEVVEFGSSEEMVDSRFPPSHSGDKVVINAKPNKLYMGPGQLYQDLQNLIHDLGLVNQISTMISSLKGNYQNVNGTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKYGRPTFFTAVFNTFSPSIKQTWISNLQMAKLALQEDNIQGWFCAEDDGNQMRKQNHPLLLKQMAVVMSKLQEFKVECAVHNPEPHVNTDSTADLPVMGHGCVWVASCTNHMGQVSIVALQNSNPKVTECFNVESRILCMTHVPLDEPLKNGEGKPENRNACDMPTVCLGMEEGSISVYKSSQRSKKVRLQHFFSPDKSSVTCLVYTSGCLYAGLVSGSLVIYSRTDDGFWVESGACVLKLGVLPVKALLVVSEHIWASSGGQIFIISTQTNTVERQFEAHQEEGMIVSHMVVAGVGIWISFSTGSTLRLFHTETLDHLQDINIATAVNNILPGQQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSFHAHHGPVKFLTMATAVCNVSQATMATLTSSTPSQCQPEASELEVATLNQNCSSGSHAPVLQDSPSSSCGSLALSQASLEHGPEDGAIYDLLNEPAHFQKAKQTDRVSASSVLVVSGGLGHRRINRKTKQSKHEEMASTIMVWQIPLPSL

Target ORF information:

RefSeq Version	XM_009293079.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293079.2

ORF Insert Sequence:

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATC TGTCTGAAGA TCTGGCACGT TTGAAAGAGC ACTATGAGAG AAAGATGCGA 
GATCTCATGG CAAACACAGT GGGAACAGTA GAGCTACAGC AGCTCAAACA CAAACACGAG 
CAGAAGATGC AGCGGCTGGT AAAAGCTGCC AAAGATGGAA CTAAAGACGG CTTGCAGAAA 
ACCAAAGCCG CAGTGAGAAA GGGCCGATCA TTCATCCGCA CCAGATCTCT CTGTCATGAT 
AAGAAACCCA ACTGCTTTGA TGAAGAGTCA GATCTATTTA TTGAGGTGGA GTGTTTTAAT 
GTGGACCCAG TGCTTGGACC TGCGCCTGAC GGACTCACTC AACACCAGGT AATGAGGAGA 
TGCATTCTGG AGAAGATTCT GGAGAGCGAG AAGAGCTATT TGGAAGCTCT GAAGAGAATT 
TTAGAGCAGT ATGAGAATCC TCTGGCAGAA ATTGAGCCTC GGTTACTCAG TGACCGCAAG 
CGTAAGGTGG TGTTTCATCG CATTCGGGAG ATCCTGCAGT GTCATGCACT CTTCCAGATG 
GCGCTTGCCA GCAGAGTATC TGACTGGGAT GAGCTGGAGA TGATTGGAGA TGTGTTTGTA 
GCATCGTTTT CCAAGTCTAT GGTGTTGGAC GCTTACAGTG AATATGTGAA TAACTTCAGC 
TTGGCGATGG GAGTGGTGCG GAAGGCATGC GCCGCTAAAC CCTGTTTCCT GGACTTCCTC 
AAGCACCGCC AGGAGACAAG TAAAGACCGT CTGACTCTTT ATGGACTGAT GATGAAGCCT 
ATTCAGCGGT TCCCTCAGTT TATTCTGCTG CTGCAAGACA TGTTAAAGAA CACGCCAGTG 
GGCCATGCTG ATCGCCTTCC CCTTCAGATG GCTCTTACTG AACTGGAAGT GCTTGCCGAA 
AAATTAAACG AAAGGAAAAA GGAGGCTGAC CAGCGCTGCG AGATACGACA CATCGCTAAA 
GCTATGAACG AACATTACCT CAACAAGCTG CTGAGCAGCG GCAGCCGTTA TCTGATTCGG 
TCTGATGACA TGGTGGAAAC CGTTTATAAT GATCGAGGGG AGATCATTAA GACTAAAGAA 
CGGCGAGTCT TTCTCCTGAA CGATGTGCTC ATGTGTGCCA CCCCCAACCG GCGGCCATCT 
CAGGATGGTG TGAGTGTTGG AGCTGGAGGT GAAGGGGTTC CCTCAGGACA GCGTTTCTTG 
CTCAAGTGGA GTGTACCGTT AAGTTTTGTG GAGGTCGTGG AGTTTGGCTC CAGCGAGGAG 
ATGGTTGACT CCCGGTTTCC ACCCTCACAC TCTGGAGATA AAGTTGTCAT TAATGCAAAA 
CCTAATAAGT TATATATGGG GCCGGGACAG CTGTATCAGG ACCTGCAGAA CCTGATCCAT 
GACCTGGGCT TGGTGAACCA AATTTCCACC ATGATCAGCA GTCTGAAAGG GAATTATCAG 
AATGTTAATG GGACTGTTGC TCAGGACTGG GTTTCAGGTC TTCAGCGTCT CATTTTAAAG 
AAAGAGGAGG AGATTCGGGC AGCTGATCGC TGTAGGATAC AACTACAGTT ACCAGGGAAA 
CAAGACAAAT ACGGTCGACC CACGTTTTTC ACTGCAGTCT TCAACACATT CAGTCCATCC 
ATCAAGCAAA CATGGATCAG TAATCTGCAG ATGGCTAAAC TGGCTTTACA GGAGGACAAC 
ATCCAAGGAT GGTTTTGTGC TGAAGATGAT GGTAATCAGA TGAGAAAGCA GAATCATCCT 
CTCTTGCTCA AGCAAATGGC AGTGGTCATG TCTAAACTAC AGGAGTTTAA GGTGGAGTGT 
GCCGTTCACA ACCCCGAGCC TCATGTGAAC ACCGACAGCA CCGCAGATCT GCCTGTTATG 
GGTCACGGAT GTGTGTGGGT GGCGAGTTGC ACCAATCACA TGGGTCAGGT GTCCATTGTG 
GCACTGCAAA ACTCAAATCC TAAAGTGACG GAGTGTTTTA ATGTCGAGTC GCGTATTCTC 
TGTATGACGC ATGTTCCGTT GGATGAGCCA CTAAAGAACG GTGAGGGAAA ACCTGAGAAT 
CGGAATGCCT GTGATATGCC AACTGTCTGT TTGGGAATGG AGGAAGGCAG TATATCTGTT 
TATAAGAGCA GCCAGCGCTC TAAGAAAGTT CGCCTGCAGC ACTTCTTCTC TCCTGATAAG 
TCTAGCGTCA CGTGTTTGGT GTACACGAGT GGGTGTCTGT ATGCAGGTCT AGTTAGTGGT 
TCTCTGGTCA TCTACTCCAG AACTGATGAC GGCTTCTGGG TCGAGTCGGG GGCCTGTGTG 
TTGAAGCTGG GGGTGTTGCC GGTTAAAGCT CTACTGGTGG TGAGCGAACA CATCTGGGCT 
TCATCAGGAG GACAGATCTT CATCATCAGC ACACAGACAA ACACGGTGGA GCGTCAGTTT 
GAAGCCCATC AGGAAGAAGG AATGATCGTG TCCCACATGG TGGTGGCTGG AGTGGGTATC 
TGGATATCCT TCAGCACCGG CTCAACCCTT CGACTCTTCC ACACCGAGAC TCTCGACCAC 
CTGCAAGACA TCAACATCGC CACAGCTGTC AATAACATAC TCCCAGGACA GCAGAGAGTC 
TCTGTGAGCA GTCTGTTGGT GTGTCACGGT CTGCTGCTAG TGGGCACTAA TCTGGGGGTC 
ACGGTGGCCC TTTCTGTGCC CAGACTACAG GGCATCCCAA AGGTCACAGG TCGAGGAATG 
GTCTCTTTTC ATGCACACCA CGGCCCAGTC AAGTTCCTCA CCATGGCAAC AGCAGTGTGT 
AACGTCTCTC AAGCTACCAT GGCAACCCTC ACCTCCAGTA CTCCCAGTCA GTGCCAGCCT 
GAGGCCTCGG AACTGGAGGT AGCCACACTA AACCAGAACT GCAGCAGCGG CTCACATGCG 
CCTGTTCTCC AGGACTCCCC GTCCTCCTCG TGTGGCTCTC TGGCTCTGTC TCAGGCATCT 
TTAGAGCACG GCCCAGAGGA CGGGGCCATA TACGACCTGC TGAACGAGCC GGCACACTTC 
CAGAAGGCCA AGCAGACTGA CAGAGTCAGT GCCAGCTCTG TGCTGGTGGT GTCAGGAGGG 
CTCGGACACC GCAGAATCAA CCGAAAAACC AAACAGTCCA AGCACGAGGA GATGGCGTCC 
ACTATCATGG TGTGGCAAAT ACCACTGCCA AGCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef10 ( XM_009293081.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291356.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa39978
Clone ID Related Accession (Same CDS sequence)	XM_009293081.2 , XM_009293081.3
Accession Version	XM_009293081.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rho guanine nucleotide exchange factor 10 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG 
AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCATTCATCC GCACCAGATC TCTCTGTCAT 
GATAAGAAAC CCAACTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT 
AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG 
AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA 
ATTTTAGAGC AGTATGAGAA TCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC 
AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG 
ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT 
GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC 
AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC 
CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG 
CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA 
GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC 
GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT 
AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT 
CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA 
GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA 
TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCCTCAGG ACAGCGTTTC 
TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG 
GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA 
AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC 
CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT 
CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA 
AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG 
AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA 
TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC 
AACATCCAAG GATGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGAAA GCAGAATCAT 
CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG 
TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT 
ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT 
GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACGGAGTGTT TTAATGTCGA GTCGCGTATT 
CTCTGTATGA CGCATGTTCC GTTGGATGAG CCACTAAAGA ACGGTGAGGG AAAACCTGAG 
AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT 
GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT 
AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTTAGT 
GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT 
GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGAGCGA ACACATCTGG 
GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG 
TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT 
ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC 
CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA 
GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG 
GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA 
ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG 
TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG 
CCTGAGGCCT CGGAACTGGA GGTAGCCACA CTAAACCAGA ACTGCAGCAG CGGCTCACAT 
GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA 
TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC 
TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA 
GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG 
TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291356.1
CDS	200..4321
Translation	MFSMDMEDFPPPPPDLLDDEDEGERFEFDDSGDEAPEADRPPPEPEGISVPPPCSSSVRDAPVNEASPNPEHTFNAEADLPPPPQDFPANNRPLAEALFKQSDLPPLPPPPPTELCPGAAEGWKIDLNPPAEGTDVHREQDENKGASVVCSSKPKVSPYSVIDIGPIQQQLPSEQSGPPSSPSANQIEGVVQDVLGSPGLSSGYSVPVPCGYATPSNVPVIPPTYTTPVLIRHFSMDEDVTVVGAVNTPVDSVFSEECPVIKEEDALTKWVSDPANTAWMESPDEVIYDDVPRENSGSTTDPDEMIYDDVEFGEEGGSSSLDNGWSSSEFESYEENSDVENGHGENGLPDAFVRGRAPSKKTHMQRLVKAAKDGTKDGLQKTKAAVRKGRSFIRTRSLCHDKKPNCFDEESDLFIEVECFNVDPVLGPAPDGLTQHQVMRRCILEKILESEKSYLEALKRILEQYENPLAEIEPRLLSDRKRKVVFHRIREILQCHALFQMALASRVSDWDELEMIGDVFVASFSKSMVLDAYSEYVNNFSLAMGVVRKACAAKPCFLDFLKHRQETSKDRLTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHADRLPLQMALTELEVLAEKLNERKKEADQRCEIRHIAKAMNEHYLNKLLSSGSRYLIRSDDMVETVYNDRGEIIKTKERRVFLLNDVLMCATPNRRPSQDGVSVGAGGEGVPSGQRFLLKWSVPLSFVEVVEFGSSEEMVDSRFPPSHSGDKVVINAKPNKLYMGPGQLYQDLQNLIHDLGLVNQISTMISSLKGNYQNVNGTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKYGRPTFFTAVFNTFSPSIKQTWISNLQMAKLALQEDNIQGWFCAEDDGNQMRKQNHPLLLKQMAVVMSKLQEFKVECAVHNPEPHVNTDSTADLPVMGHGCVWVASCTNHMGQVSIVALQNSNPKVTECFNVESRILCMTHVPLDEPLKNGEGKPENRNACDMPTVCLGMEEGSISVYKSSQRSKKVRLQHFFSPDKSSVTCLVYTSGCLYAGLVSGSLVIYSRTDDGFWVESGACVLKLGVLPVKALLVVSEHIWASSGGQIFIISTQTNTVERQFEAHQEEGMIVSHMVVAGVGIWISFSTGSTLRLFHTETLDHLQDINIATAVNNILPGQQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSFHAHHGPVKFLTMATAVCNVSQATMATLTSSTPSQCQPEASELEVATLNQNCSSGSHAPVLQDSPSSSCGSLALSQASLEHGPEDGAIYDLLNEPAHFQKAKQTDRVSASSVLVVSGGLGHRRINRKTKQSKHEEMASTIMVWQIPLPSL

Target ORF information:

RefSeq Version	XM_009293081.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293081.2

ORF Insert Sequence:

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG 
AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCATTCATCC GCACCAGATC TCTCTGTCAT 
GATAAGAAAC CCAACTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT 
AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG 
AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA 
ATTTTAGAGC AGTATGAGAA TCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC 
AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG 
ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT 
GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC 
AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC 
CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG 
CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA 
GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC 
GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT 
AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT 
CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA 
GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA 
TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCCTCAGG ACAGCGTTTC 
TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG 
GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA 
AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC 
CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT 
CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA 
AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG 
AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA 
TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC 
AACATCCAAG GATGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGAAA GCAGAATCAT 
CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG 
TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT 
ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT 
GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACGGAGTGTT TTAATGTCGA GTCGCGTATT 
CTCTGTATGA CGCATGTTCC GTTGGATGAG CCACTAAAGA ACGGTGAGGG AAAACCTGAG 
AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT 
GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT 
AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTTAGT 
GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT 
GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGAGCGA ACACATCTGG 
GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG 
TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT 
ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC 
CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA 
GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG 
GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA 
ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG 
TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG 
CCTGAGGCCT CGGAACTGGA GGTAGCCACA CTAAACCAGA ACTGCAGCAG CGGCTCACAT 
GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA 
TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC 
TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA 
GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG 
TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef10 ( XM_009293081.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291356.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

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CloneID	ODa39978
Clone ID Related Accession (Same CDS sequence)	XM_009293081.2 , XM_009293081.3
Accession Version	XM_009293081.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	rho guanine nucleotide exchange factor 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG 
AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCATTCATCC GCACCAGATC TCTCTGTCAT 
GATAAGAAAC CCAACTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT 
AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG 
AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA 
ATTTTAGAGC AGTATGAGAA TCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC 
AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG 
ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT 
GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC 
AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC 
CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG 
CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA 
GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC 
GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT 
AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT 
CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA 
GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA 
TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCCTCAGG ACAGCGTTTC 
TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG 
GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA 
AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC 
CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT 
CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA 
AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG 
AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA 
TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC 
AACATCCAAG GATGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGAAA GCAGAATCAT 
CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG 
TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT 
ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT 
GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACGGAGTGTT TTAATGTCGA GTCGCGTATT 
CTCTGTATGA CGCATGTTCC GTTGGATGAG CCACTAAAGA ACGGTGAGGG AAAACCTGAG 
AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT 
GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT 
AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTTAGT 
GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT 
GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGAGCGA ACACATCTGG 
GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG 
TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT 
ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC 
CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA 
GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG 
GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA 
ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG 
TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG 
CCTGAGGCCT CGGAACTGGA GGTAGCCACA CTAAACCAGA ACTGCAGCAG CGGCTCACAT 
GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA 
TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC 
TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA 
GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG 
TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291356.1
CDS	204..4325
Translation	MFSMDMEDFPPPPPDLLDDEDEGERFEFDDSGDEAPEADRPPPEPEGISVPPPCSSSVRDAPVNEASPNPEHTFNAEADLPPPPQDFPANNRPLAEALFKQSDLPPLPPPPPTELCPGAAEGWKIDLNPPAEGTDVHREQDENKGASVVCSSKPKVSPYSVIDIGPIQQQLPSEQSGPPSSPSANQIEGVVQDVLGSPGLSSGYSVPVPCGYATPSNVPVIPPTYTTPVLIRHFSMDEDVTVVGAVNTPVDSVFSEECPVIKEEDALTKWVSDPANTAWMESPDEVIYDDVPRENSGSTTDPDEMIYDDVEFGEEGGSSSLDNGWSSSEFESYEENSDVENGHGENGLPDAFVRGRAPSKKTHMQRLVKAAKDGTKDGLQKTKAAVRKGRSFIRTRSLCHDKKPNCFDEESDLFIEVECFNVDPVLGPAPDGLTQHQVMRRCILEKILESEKSYLEALKRILEQYENPLAEIEPRLLSDRKRKVVFHRIREILQCHALFQMALASRVSDWDELEMIGDVFVASFSKSMVLDAYSEYVNNFSLAMGVVRKACAAKPCFLDFLKHRQETSKDRLTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHADRLPLQMALTELEVLAEKLNERKKEADQRCEIRHIAKAMNEHYLNKLLSSGSRYLIRSDDMVETVYNDRGEIIKTKERRVFLLNDVLMCATPNRRPSQDGVSVGAGGEGVPSGQRFLLKWSVPLSFVEVVEFGSSEEMVDSRFPPSHSGDKVVINAKPNKLYMGPGQLYQDLQNLIHDLGLVNQISTMISSLKGNYQNVNGTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKYGRPTFFTAVFNTFSPSIKQTWISNLQMAKLALQEDNIQGWFCAEDDGNQMRKQNHPLLLKQMAVVMSKLQEFKVECAVHNPEPHVNTDSTADLPVMGHGCVWVASCTNHMGQVSIVALQNSNPKVTECFNVESRILCMTHVPLDEPLKNGEGKPENRNACDMPTVCLGMEEGSISVYKSSQRSKKVRLQHFFSPDKSSVTCLVYTSGCLYAGLVSGSLVIYSRTDDGFWVESGACVLKLGVLPVKALLVVSEHIWASSGGQIFIISTQTNTVERQFEAHQEEGMIVSHMVVAGVGIWISFSTGSTLRLFHTETLDHLQDINIATAVNNILPGQQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSFHAHHGPVKFLTMATAVCNVSQATMATLTSSTPSQCQPEASELEVATLNQNCSSGSHAPVLQDSPSSSCGSLALSQASLEHGPEDGAIYDLLNEPAHFQKAKQTDRVSASSVLVVSGGLGHRRINRKTKQSKHEEMASTIMVWQIPLPSL

Target ORF information:

RefSeq Version	XM_009293081.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293081.3

ORF Insert Sequence:

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGAAAACA AAGGTGCCAG TGTGGTTTGC TCCTCCAAGC CCAAAGTGAG CCCCTACTCT 
GTGATCGACA TCGGCCCCAT CCAACAGCAG CTGCCATCAG AGCAGAGCGG TCCGCCCTCC 
TCTCCCTCAG CCAATCAGAT TGAAGGAGTT GTTCAGGATG TGTTAGGTAG CCCAGGGTTG 
TCCTCTGGGT ATTCAGTGCC AGTGCCCTGT GGTTATGCAA CGCCTTCAAA TGTGCCAGTG 
ATCCCACCAA CCTACACCAC CCCAGTTCTC ATCCGCCACT TCTCTATGGA TGAAGATGTA 
ACTGTTGTTG GAGCAGTAAA TACTCCTGTG GACAGTGTTT TTAGTGAAGA GTGTCCGGTC 
ATCAAAGAGG AAGACGCTCT TACTAAGTGG GTTTCAGACC CAGCAAACAC AGCCTGGATG 
GAGAGTCCAG ATGAAGTGAT TTATGATGAT GTACCAAGAG AAAACTCTGG ATCCACAACA 
GACCCAGATG AGATGATCTA TGATGACGTT GAGTTCGGGG AGGAGGGTGG CAGCAGCTCG 
CTGGACAATG GCTGGAGCTC CAGCGAATTT GAGAGCTATG AGGAAAACAG TGACGTAGAG 
AACGGACATG GAGAAAACGG CCTCCCGGAC GCCTTCGTCA GGGGCCGTGC ACCCAGCAAG 
AAAACACATA TGCAGCGGCT GGTAAAAGCT GCCAAAGATG GAACTAAAGA CGGCTTGCAG 
AAAACCAAAG CCGCAGTGAG AAAGGGCCGA TCATTCATCC GCACCAGATC TCTCTGTCAT 
GATAAGAAAC CCAACTGCTT TGATGAAGAG TCAGATCTAT TTATTGAGGT GGAGTGTTTT 
AATGTGGACC CAGTGCTTGG ACCTGCGCCT GACGGACTCA CTCAACACCA GGTAATGAGG 
AGATGCATTC TGGAGAAGAT TCTGGAGAGC GAGAAGAGCT ATTTGGAAGC TCTGAAGAGA 
ATTTTAGAGC AGTATGAGAA TCCTCTGGCA GAAATTGAGC CTCGGTTACT CAGTGACCGC 
AAGCGTAAGG TGGTGTTTCA TCGCATTCGG GAGATCCTGC AGTGTCATGC ACTCTTCCAG 
ATGGCGCTTG CCAGCAGAGT ATCTGACTGG GATGAGCTGG AGATGATTGG AGATGTGTTT 
GTAGCATCGT TTTCCAAGTC TATGGTGTTG GACGCTTACA GTGAATATGT GAATAACTTC 
AGCTTGGCGA TGGGAGTGGT GCGGAAGGCA TGCGCCGCTA AACCCTGTTT CCTGGACTTC 
CTCAAGCACC GCCAGGAGAC AAGTAAAGAC CGTCTGACTC TTTATGGACT GATGATGAAG 
CCTATTCAGC GGTTCCCTCA GTTTATTCTG CTGCTGCAAG ACATGTTAAA GAACACGCCA 
GTGGGCCATG CTGATCGCCT TCCCCTTCAG ATGGCTCTTA CTGAACTGGA AGTGCTTGCC 
GAAAAATTAA ACGAAAGGAA AAAGGAGGCT GACCAGCGCT GCGAGATACG ACACATCGCT 
AAAGCTATGA ACGAACATTA CCTCAACAAG CTGCTGAGCA GCGGCAGCCG TTATCTGATT 
CGGTCTGATG ACATGGTGGA AACCGTTTAT AATGATCGAG GGGAGATCAT TAAGACTAAA 
GAACGGCGAG TCTTTCTCCT GAACGATGTG CTCATGTGTG CCACCCCCAA CCGGCGGCCA 
TCTCAGGATG GTGTGAGTGT TGGAGCTGGA GGTGAAGGGG TTCCCTCAGG ACAGCGTTTC 
TTGCTCAAGT GGAGTGTACC GTTAAGTTTT GTGGAGGTCG TGGAGTTTGG CTCCAGCGAG 
GAGATGGTTG ACTCCCGGTT TCCACCCTCA CACTCTGGAG ATAAAGTTGT CATTAATGCA 
AAACCTAATA AGTTATATAT GGGGCCGGGA CAGCTGTATC AGGACCTGCA GAACCTGATC 
CATGACCTGG GCTTGGTGAA CCAAATTTCC ACCATGATCA GCAGTCTGAA AGGGAATTAT 
CAGAATGTTA ATGGGACTGT TGCTCAGGAC TGGGTTTCAG GTCTTCAGCG TCTCATTTTA 
AAGAAAGAGG AGGAGATTCG GGCAGCTGAT CGCTGTAGGA TACAACTACA GTTACCAGGG 
AAACAAGACA AATACGGTCG ACCCACGTTT TTCACTGCAG TCTTCAACAC ATTCAGTCCA 
TCCATCAAGC AAACATGGAT CAGTAATCTG CAGATGGCTA AACTGGCTTT ACAGGAGGAC 
AACATCCAAG GATGGTTTTG TGCTGAAGAT GATGGTAATC AGATGAGAAA GCAGAATCAT 
CCTCTCTTGC TCAAGCAAAT GGCAGTGGTC ATGTCTAAAC TACAGGAGTT TAAGGTGGAG 
TGTGCCGTTC ACAACCCCGA GCCTCATGTG AACACCGACA GCACCGCAGA TCTGCCTGTT 
ATGGGTCACG GATGTGTGTG GGTGGCGAGT TGCACCAATC ACATGGGTCA GGTGTCCATT 
GTGGCACTGC AAAACTCAAA TCCTAAAGTG ACGGAGTGTT TTAATGTCGA GTCGCGTATT 
CTCTGTATGA CGCATGTTCC GTTGGATGAG CCACTAAAGA ACGGTGAGGG AAAACCTGAG 
AATCGGAATG CCTGTGATAT GCCAACTGTC TGTTTGGGAA TGGAGGAAGG CAGTATATCT 
GTTTATAAGA GCAGCCAGCG CTCTAAGAAA GTTCGCCTGC AGCACTTCTT CTCTCCTGAT 
AAGTCTAGCG TCACGTGTTT GGTGTACACG AGTGGGTGTC TGTATGCAGG TCTAGTTAGT 
GGTTCTCTGG TCATCTACTC CAGAACTGAT GACGGCTTCT GGGTCGAGTC GGGGGCCTGT 
GTGTTGAAGC TGGGGGTGTT GCCGGTTAAA GCTCTACTGG TGGTGAGCGA ACACATCTGG 
GCTTCATCAG GAGGACAGAT CTTCATCATC AGCACACAGA CAAACACGGT GGAGCGTCAG 
TTTGAAGCCC ATCAGGAAGA AGGAATGATC GTGTCCCACA TGGTGGTGGC TGGAGTGGGT 
ATCTGGATAT CCTTCAGCAC CGGCTCAACC CTTCGACTCT TCCACACCGA GACTCTCGAC 
CACCTGCAAG ACATCAACAT CGCCACAGCT GTCAATAACA TACTCCCAGG ACAGCAGAGA 
GTCTCTGTGA GCAGTCTGTT GGTGTGTCAC GGTCTGCTGC TAGTGGGCAC TAATCTGGGG 
GTCACGGTGG CCCTTTCTGT GCCCAGACTA CAGGGCATCC CAAAGGTCAC AGGTCGAGGA 
ATGGTCTCTT TTCATGCACA CCACGGCCCA GTCAAGTTCC TCACCATGGC AACAGCAGTG 
TGTAACGTCT CTCAAGCTAC CATGGCAACC CTCACCTCCA GTACTCCCAG TCAGTGCCAG 
CCTGAGGCCT CGGAACTGGA GGTAGCCACA CTAAACCAGA ACTGCAGCAG CGGCTCACAT 
GCGCCTGTTC TCCAGGACTC CCCGTCCTCC TCGTGTGGCT CTCTGGCTCT GTCTCAGGCA 
TCTTTAGAGC ACGGCCCAGA GGACGGGGCC ATATACGACC TGCTGAACGA GCCGGCACAC 
TTCCAGAAGG CCAAGCAGAC TGACAGAGTC AGTGCCAGCT CTGTGCTGGT GGTGTCAGGA 
GGGCTCGGAC ACCGCAGAAT CAACCGAAAA ACCAAACAGT CCAAGCACGA GGAGATGGCG 
TCCACTATCA TGGTGTGGCA AATACCACTG CCAAGCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef10 ( XM_009293082.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291357.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA.

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CloneID	ODa39979
Clone ID Related Accession (Same CDS sequence)	XM_009293082.2
Accession Version	XM_009293082.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	rho guanine nucleotide exchange factor 10 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGTAACTG TTGTTGGAGC AGTAAATACT CCTGTGGACA GTGTTTTTAG TGAAGAGTGT 
CCGGTCATCA AAGAGGAAGA CGCTCTTACT AAGTGGGTTT CAGACCCAGC AAACACAGCC 
TGGATGGAGA GTCCAGATGA AGTGATTTAT GATGATGTAC CAAGAGAAAA CTCTGGATCC 
ACAACAGACC CAGATGAGAT GATCTATGAT GACGTTGAGT TCGGGGAGGA GGGTGGCAGC 
AGCTCGCTGG ACAATGGCTG GAGCTCCAGC GAATTTGAGA GCTATGAGGA AAACAGTGAC 
GTAGAGAACG GACATGGAGA AAACGGCCTC CCGGACGCCT TCGTCAGGGG CCGTGCACCC 
AGCAAGAAAA CACATCTGTC TGAAGATCTG GCACGTTTGA AAGAGCACTA TGAGAGAAAG 
ATGCGAGATC TCATGGCAAA CACAGTGGGA ACAGTAGAGC TACAGCAGCT CAAACACAAA 
CACGAGCAGA AGATGCAGCG GCTGGTAAAA GCTGCCAAAG ATGGAACTAA AGACGGCTTG 
CAGAAAACCA AAGCCGCAGT GAGAAAGGGC CGATCATTCA TCCGCACCAG ATCTCTCTGT 
CATGATAAGA AACCCAACTG CTTTGATGAA GAGTCAGATC TATTTATTGA GGTGGAGTGT 
TTTAATGTGG ACCCAGTGCT TGGACCTGCG CCTGACGGAC TCACTCAACA CCAGGTAATG 
AGGAGATGCA TTCTGGAGAA GATTCTGGAG AGCGAGAAGA GCTATTTGGA AGCTCTGAAG 
AGAATTTTAG AGCAGTATGA GAATCCTCTG GCAGAAATTG AGCCTCGGTT ACTCAGTGAC 
CGCAAGCGTA AGGTGGTGTT TCATCGCATT CGGGAGATCC TGCAGTGTCA TGCACTCTTC 
CAGATGGCGC TTGCCAGCAG AGTATCTGAC TGGGATGAGC TGGAGATGAT TGGAGATGTG 
TTTGTAGCAT CGTTTTCCAA GTCTATGGTG TTGGACGCTT ACAGTGAATA TGTGAATAAC 
TTCAGCTTGG CGATGGGAGT GGTGCGGAAG GCATGCGCCG CTAAACCCTG TTTCCTGGAC 
TTCCTCAAGC ACCGCCAGGA GACAAGTAAA GACCGTCTGA CTCTTTATGG ACTGATGATG 
AAGCCTATTC AGCGGTTCCC TCAGTTTATT CTGCTGCTGC AAGACATGTT AAAGAACACG 
CCAGTGGGCC ATGCTGATCG CCTTCCCCTT CAGATGGCTC TTACTGAACT GGAAGTGCTT 
GCCGAAAAAT TAAACGAAAG GAAAAAGGAG GCTGACCAGC GCTGCGAGAT ACGACACATC 
GCTAAAGCTA TGAACGAACA TTACCTCAAC AAGCTGCTGA GCAGCGGCAG CCGTTATCTG 
ATTCGGTCTG ATGACATGGT GGAAACCGTT TATAATGATC GAGGGGAGAT CATTAAGACT 
AAAGAACGGC GAGTCTTTCT CCTGAACGAT GTGCTCATGT GTGCCACCCC CAACCGGCGG 
CCATCTCAGG ATGGTGTGAG TGTTGGAGCT GGAGGTGAAG GGGTTCCCTC AGGACAGCGT 
TTCTTGCTCA AGTGGAGTGT ACCGTTAAGT TTTGTGGAGG TCGTGGAGTT TGGCTCCAGC 
GAGGAGATGG TTGACTCCCG GTTTCCACCC TCACACTCTG GAGATAAAGT TGTCATTAAT 
GCAAAACCTA ATAAGTTATA TATGGGGCCG GGACAGCTGT ATCAGGACCT GCAGAACCTG 
ATCCATGACC TGGGCTTGGT GAACCAAATT TCCACCATGA TCAGCAGTCT GAAAGGGAAT 
TATCAGAATG TTAATGGGAC TGTTGCTCAG GACTGGGTTT CAGGTCTTCA GCGTCTCATT 
TTAAAGAAAG AGGAGGAGAT TCGGGCAGCT GATCGCTGTA GGATACAACT ACAGTTACCA 
GGGAAACAAG ACAAATACGG TCGACCCACG TTTTTCACTG CAGTCTTCAA CACATTCAGT 
CCATCCATCA AGCAAACATG GATCAGTAAT CTGCAGATGG CTAAACTGGC TTTACAGGAG 
GACAACATCC AAGGATGGTT TTGTGCTGAA GATGATGGTA ATCAGATGAG AAAGCAGAAT 
CATCCTCTCT TGCTCAAGCA AATGGCAGTG GTCATGTCTA AACTACAGGA GTTTAAGGTG 
GAGTGTGCCG TTCACAACCC CGAGCCTCAT GTGAACACCG ACAGCACCGC AGATCTGCCT 
GTTATGGGTC ACGGATGTGT GTGGGTGGCG AGTTGCACCA ATCACATGGG TCAGGTGTCC 
ATTGTGGCAC TGCAAAACTC AAATCCTAAA GTGACGGAGT GTTTTAATGT CGAGTCGCGT 
ATTCTCTGTA TGACGCATGT TCCGTTGGAT GAGCCACTAA AGAACGGTGA GGGAAAACCT 
GAGAATCGGA ATGCCTGTGA TATGCCAACT GTCTGTTTGG GAATGGAGGA AGGCAGTATA 
TCTGTTTATA AGAGCAGCCA GCGCTCTAAG AAAGTTCGCC TGCAGCACTT CTTCTCTCCT 
GATAAGTCTA GCGTCACGTG TTTGGTGTAC ACGAGTGGGT GTCTGTATGC AGGTCTAGTT 
AGTGGTTCTC TGGTCATCTA CTCCAGAACT GATGACGGCT TCTGGGTCGA GTCGGGGGCC 
TGTGTGTTGA AGCTGGGGGT GTTGCCGGTT AAAGCTCTAC TGGTGGTGAG CGAACACATC 
TGGGCTTCAT CAGGAGGACA GATCTTCATC ATCAGCACAC AGACAAACAC GGTGGAGCGT 
CAGTTTGAAG CCCATCAGGA AGAAGGAATG ATCGTGTCCC ACATGGTGGT GGCTGGAGTG 
GGTATCTGGA TATCCTTCAG CACCGGCTCA ACCCTTCGAC TCTTCCACAC CGAGACTCTC 
GACCACCTGC AAGACATCAA CATCGCCACA GCTGTCAATA ACATACTCCC AGGACAGCAG 
AGAGTCTCTG TGAGCAGTCT GTTGGTGTGT CACGGTCTGC TGCTAGTGGG CACTAATCTG 
GGGGTCACGG TGGCCCTTTC TGTGCCCAGA CTACAGGGCA TCCCAAAGGT CACAGGTCGA 
GGAATGGTCT CTTTTCATGC ACACCACGGC CCAGTCAAGT TCCTCACCAT GGCAACAGCA 
GTGTGTAACG TCTCTCAAGC TACCATGGCA ACCCTCACCT CCAGTACTCC CAGTCAGTGC 
CAGCCTGAGG CCTCGGAACT GGAGGTAGCC ACACTAAACC AGAACTGCAG CAGCGGCTCA 
CATGCGCCTG TTCTCCAGGA CTCCCCGTCC TCCTCGTGTG GCTCTCTGGC TCTGTCTCAG 
GCATCTTTAG AGCACGGCCC AGAGGACGGG GCCATATACG ACCTGCTGAA CGAGCCGGCA 
CACTTCCAGA AGGCCAAGCA GACTGACAGA GTCAGTGCCA GCTCTGTGCT GGTGGTGTCA 
GGAGGGCTCG GACACCGCAG AATCAACCGA AAAACCAAAC AGTCCAAGCA CGAGGAGATG 
GCGTCCACTA TCATGGTGTG GCAAATACCA CTGCCAAGCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291357.1
CDS	199..4143
Translation	MFSMDMEDFPPPPPDLLDDEDEGERFEFDDSGDEAPEADRPPPEPEGISVPPPCSSSVRDAPVNEASPNPEHTFNAEADLPPPPQDFPANNRPLAEALFKQSDLPPLPPPPPTELCPGAAEGWKIDLNPPAEGTDVHREQDVTVVGAVNTPVDSVFSEECPVIKEEDALTKWVSDPANTAWMESPDEVIYDDVPRENSGSTTDPDEMIYDDVEFGEEGGSSSLDNGWSSSEFESYEENSDVENGHGENGLPDAFVRGRAPSKKTHLSEDLARLKEHYERKMRDLMANTVGTVELQQLKHKHEQKMQRLVKAAKDGTKDGLQKTKAAVRKGRSFIRTRSLCHDKKPNCFDEESDLFIEVECFNVDPVLGPAPDGLTQHQVMRRCILEKILESEKSYLEALKRILEQYENPLAEIEPRLLSDRKRKVVFHRIREILQCHALFQMALASRVSDWDELEMIGDVFVASFSKSMVLDAYSEYVNNFSLAMGVVRKACAAKPCFLDFLKHRQETSKDRLTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHADRLPLQMALTELEVLAEKLNERKKEADQRCEIRHIAKAMNEHYLNKLLSSGSRYLIRSDDMVETVYNDRGEIIKTKERRVFLLNDVLMCATPNRRPSQDGVSVGAGGEGVPSGQRFLLKWSVPLSFVEVVEFGSSEEMVDSRFPPSHSGDKVVINAKPNKLYMGPGQLYQDLQNLIHDLGLVNQISTMISSLKGNYQNVNGTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKYGRPTFFTAVFNTFSPSIKQTWISNLQMAKLALQEDNIQGWFCAEDDGNQMRKQNHPLLLKQMAVVMSKLQEFKVECAVHNPEPHVNTDSTADLPVMGHGCVWVASCTNHMGQVSIVALQNSNPKVTECFNVESRILCMTHVPLDEPLKNGEGKPENRNACDMPTVCLGMEEGSISVYKSSQRSKKVRLQHFFSPDKSSVTCLVYTSGCLYAGLVSGSLVIYSRTDDGFWVESGACVLKLGVLPVKALLVVSEHIWASSGGQIFIISTQTNTVERQFEAHQEEGMIVSHMVVAGVGIWISFSTGSTLRLFHTETLDHLQDINIATAVNNILPGQQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSFHAHHGPVKFLTMATAVCNVSQATMATLTSSTPSQCQPEASELEVATLNQNCSSGSHAPVLQDSPSSSCGSLALSQASLEHGPEDGAIYDLLNEPAHFQKAKQTDRVSASSVLVVSGGLGHRRINRKTKQSKHEEMASTIMVWQIPLPSL

Target ORF information:

RefSeq Version	XM_009293082.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293082.2

ORF Insert Sequence:

ATGTTCAGCA TGGACATGGA GGATTTCCCC CCTCCTCCAC CTGACCTGTT GGATGACGAA 
GATGAAGGTG AACGATTTGA GTTTGATGAC AGTGGTGATG AAGCCCCCGA GGCGGATCGG 
CCTCCACCTG AACCTGAGGG AATCTCCGTT CCTCCACCCT GCTCTTCCTC AGTCAGAGAC 
GCACCCGTGA ATGAGGCAAG TCCAAACCCA GAGCACACAT TCAACGCAGA GGCAGATTTG 
CCCCCTCCGC CTCAGGACTT CCCCGCTAAT AACAGGCCTC TGGCTGAAGC GCTGTTCAAA 
CAGTCTGATC TCCCTCCTCT TCCTCCTCCT CCTCCCACTG AACTTTGTCC CGGTGCAGCA 
GAGGGCTGGA AAATAGACCT CAATCCACCA GCAGAGGGCA CTGATGTTCA CAGAGAGCAA 
GACGTAACTG TTGTTGGAGC AGTAAATACT CCTGTGGACA GTGTTTTTAG TGAAGAGTGT 
CCGGTCATCA AAGAGGAAGA CGCTCTTACT AAGTGGGTTT CAGACCCAGC AAACACAGCC 
TGGATGGAGA GTCCAGATGA AGTGATTTAT GATGATGTAC CAAGAGAAAA CTCTGGATCC 
ACAACAGACC CAGATGAGAT GATCTATGAT GACGTTGAGT TCGGGGAGGA GGGTGGCAGC 
AGCTCGCTGG ACAATGGCTG GAGCTCCAGC GAATTTGAGA GCTATGAGGA AAACAGTGAC 
GTAGAGAACG GACATGGAGA AAACGGCCTC CCGGACGCCT TCGTCAGGGG CCGTGCACCC 
AGCAAGAAAA CACATCTGTC TGAAGATCTG GCACGTTTGA AAGAGCACTA TGAGAGAAAG 
ATGCGAGATC TCATGGCAAA CACAGTGGGA ACAGTAGAGC TACAGCAGCT CAAACACAAA 
CACGAGCAGA AGATGCAGCG GCTGGTAAAA GCTGCCAAAG ATGGAACTAA AGACGGCTTG 
CAGAAAACCA AAGCCGCAGT GAGAAAGGGC CGATCATTCA TCCGCACCAG ATCTCTCTGT 
CATGATAAGA AACCCAACTG CTTTGATGAA GAGTCAGATC TATTTATTGA GGTGGAGTGT 
TTTAATGTGG ACCCAGTGCT TGGACCTGCG CCTGACGGAC TCACTCAACA CCAGGTAATG 
AGGAGATGCA TTCTGGAGAA GATTCTGGAG AGCGAGAAGA GCTATTTGGA AGCTCTGAAG 
AGAATTTTAG AGCAGTATGA GAATCCTCTG GCAGAAATTG AGCCTCGGTT ACTCAGTGAC 
CGCAAGCGTA AGGTGGTGTT TCATCGCATT CGGGAGATCC TGCAGTGTCA TGCACTCTTC 
CAGATGGCGC TTGCCAGCAG AGTATCTGAC TGGGATGAGC TGGAGATGAT TGGAGATGTG 
TTTGTAGCAT CGTTTTCCAA GTCTATGGTG TTGGACGCTT ACAGTGAATA TGTGAATAAC 
TTCAGCTTGG CGATGGGAGT GGTGCGGAAG GCATGCGCCG CTAAACCCTG TTTCCTGGAC 
TTCCTCAAGC ACCGCCAGGA GACAAGTAAA GACCGTCTGA CTCTTTATGG ACTGATGATG 
AAGCCTATTC AGCGGTTCCC TCAGTTTATT CTGCTGCTGC AAGACATGTT AAAGAACACG 
CCAGTGGGCC ATGCTGATCG CCTTCCCCTT CAGATGGCTC TTACTGAACT GGAAGTGCTT 
GCCGAAAAAT TAAACGAAAG GAAAAAGGAG GCTGACCAGC GCTGCGAGAT ACGACACATC 
GCTAAAGCTA TGAACGAACA TTACCTCAAC AAGCTGCTGA GCAGCGGCAG CCGTTATCTG 
ATTCGGTCTG ATGACATGGT GGAAACCGTT TATAATGATC GAGGGGAGAT CATTAAGACT 
AAAGAACGGC GAGTCTTTCT CCTGAACGAT GTGCTCATGT GTGCCACCCC CAACCGGCGG 
CCATCTCAGG ATGGTGTGAG TGTTGGAGCT GGAGGTGAAG GGGTTCCCTC AGGACAGCGT 
TTCTTGCTCA AGTGGAGTGT ACCGTTAAGT TTTGTGGAGG TCGTGGAGTT TGGCTCCAGC 
GAGGAGATGG TTGACTCCCG GTTTCCACCC TCACACTCTG GAGATAAAGT TGTCATTAAT 
GCAAAACCTA ATAAGTTATA TATGGGGCCG GGACAGCTGT ATCAGGACCT GCAGAACCTG 
ATCCATGACC TGGGCTTGGT GAACCAAATT TCCACCATGA TCAGCAGTCT GAAAGGGAAT 
TATCAGAATG TTAATGGGAC TGTTGCTCAG GACTGGGTTT CAGGTCTTCA GCGTCTCATT 
TTAAAGAAAG AGGAGGAGAT TCGGGCAGCT GATCGCTGTA GGATACAACT ACAGTTACCA 
GGGAAACAAG ACAAATACGG TCGACCCACG TTTTTCACTG CAGTCTTCAA CACATTCAGT 
CCATCCATCA AGCAAACATG GATCAGTAAT CTGCAGATGG CTAAACTGGC TTTACAGGAG 
GACAACATCC AAGGATGGTT TTGTGCTGAA GATGATGGTA ATCAGATGAG AAAGCAGAAT 
CATCCTCTCT TGCTCAAGCA AATGGCAGTG GTCATGTCTA AACTACAGGA GTTTAAGGTG 
GAGTGTGCCG TTCACAACCC CGAGCCTCAT GTGAACACCG ACAGCACCGC AGATCTGCCT 
GTTATGGGTC ACGGATGTGT GTGGGTGGCG AGTTGCACCA ATCACATGGG TCAGGTGTCC 
ATTGTGGCAC TGCAAAACTC AAATCCTAAA GTGACGGAGT GTTTTAATGT CGAGTCGCGT 
ATTCTCTGTA TGACGCATGT TCCGTTGGAT GAGCCACTAA AGAACGGTGA GGGAAAACCT 
GAGAATCGGA ATGCCTGTGA TATGCCAACT GTCTGTTTGG GAATGGAGGA AGGCAGTATA 
TCTGTTTATA AGAGCAGCCA GCGCTCTAAG AAAGTTCGCC TGCAGCACTT CTTCTCTCCT 
GATAAGTCTA GCGTCACGTG TTTGGTGTAC ACGAGTGGGT GTCTGTATGC AGGTCTAGTT 
AGTGGTTCTC TGGTCATCTA CTCCAGAACT GATGACGGCT TCTGGGTCGA GTCGGGGGCC 
TGTGTGTTGA AGCTGGGGGT GTTGCCGGTT AAAGCTCTAC TGGTGGTGAG CGAACACATC 
TGGGCTTCAT CAGGAGGACA GATCTTCATC ATCAGCACAC AGACAAACAC GGTGGAGCGT 
CAGTTTGAAG CCCATCAGGA AGAAGGAATG ATCGTGTCCC ACATGGTGGT GGCTGGAGTG 
GGTATCTGGA TATCCTTCAG CACCGGCTCA ACCCTTCGAC TCTTCCACAC CGAGACTCTC 
GACCACCTGC AAGACATCAA CATCGCCACA GCTGTCAATA ACATACTCCC AGGACAGCAG 
AGAGTCTCTG TGAGCAGTCT GTTGGTGTGT CACGGTCTGC TGCTAGTGGG CACTAATCTG 
GGGGTCACGG TGGCCCTTTC TGTGCCCAGA CTACAGGGCA TCCCAAAGGT CACAGGTCGA 
GGAATGGTCT CTTTTCATGC ACACCACGGC CCAGTCAAGT TCCTCACCAT GGCAACAGCA 
GTGTGTAACG TCTCTCAAGC TACCATGGCA ACCCTCACCT CCAGTACTCC CAGTCAGTGC 
CAGCCTGAGG CCTCGGAACT GGAGGTAGCC ACACTAAACC AGAACTGCAG CAGCGGCTCA 
CATGCGCCTG TTCTCCAGGA CTCCCCGTCC TCCTCGTGTG GCTCTCTGGC TCTGTCTCAG 
GCATCTTTAG AGCACGGCCC AGAGGACGGG GCCATATACG ACCTGCTGAA CGAGCCGGCA 
CACTTCCAGA AGGCCAAGCA GACTGACAGA GTCAGTGCCA GCTCTGTGCT GGTGGTGTCA 
GGAGGGCTCG GACACCGCAG AATCAACCGA AAAACCAAAC AGTCCAAGCA CGAGGAGATG 
GCGTCCACTA TCATGGTGTG GCAAATACCA CTGCCAAGCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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arhgef10 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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