ercc4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ercc4
Entrez Gene ID	327322
Full Name	excision repair cross-complementation group 4
Synonyms	fi03a05,wu:fi03a05,zgc:63468
General protein information	Preferred Names excision repair cross-complementation group 4 Names DNA repair endonuclease XPF XPF excision repair cross-complementing rodent repair deficiency, complementation group 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199785.1	NP_956079.1	DNA repair endonuclease XPF

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RAD1	NP_015303.1
Arabidopsis thaliana (thale cress)	UVH1	NP_198931.1
Gallus gallus (chicken)	ERCC4	XP_414734.3
Drosophila melanogaster (fruit fly)	mei-9	NP_525068.1
Neurospora crassa	NCU07440	XP_964627.2
Magnaporthe oryzae (rice blast fungus)	MGG_02804	XP_003720937.1
Bos taurus (cattle)	ERCC4	NP_001192289.1
Mus musculus (house mouse)	Ercc4	NP_056584.2
Oryza sativa (rice)	Os03g0100400	NP_001048645.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002805	XP_563334.4
Xenopus tropicalis (tropical clawed frog)	ercc4	XP_002935520.2
Pan troglodytes (chimpanzee)	ERCC4	XP_510831.2
Macaca mulatta (Rhesus monkey)	ERCC4	XP_001107209.1
Rattus norvegicus (Norway rat)	Ercc4	XP_006245762.1
Schizosaccharomyces pombe (fission yeast)	rad16	NP_587855.2
Kluyveromyces lactis	KLLA0D12210g	XP_453606.1
Homo sapiens (human)	ERCC4	NP_005227.1
Canis lupus familiaris (dog)	ERCC4	XP_536967.3
Rattus norvegicus (Norway rat)	Ercc4l1	XP_003750828.1
Danio rerio (zebrafish)	ercc4	NP_956079.1
Eremothecium gossypii	AGOS_AFR456C	NP_986003.1

Gene Ontology
Bibliography

Related articles in PubMed

Multiplexed CRISPR/Cas9-mediated knockout of 19 Fanconi anemia pathway genes in zebrafish revealed their roles in growth, sexual development and fertility.
Ramanagoudr-Bhojappa R, Carrington B, Ramaswami M, Bishop K, Robbins GM, Jones M, Harper U, Frederickson SC, Kimble DC, Sood R, Chandrasekharappa SC
PLoS genetics14(12)e1007821(2018 12)

Mercury (II) impairs nucleotide excision repair (NER) in zebrafish (Danio rerio) embryos by targeting primarily at the stage of DNA incision.
Chang Y, Lee WY, Lin YJ, Hsu T
Aquatic toxicology (Amsterdam, Netherlands)19297-104(2017 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The timing of Timezyme diversification in vertebrates.
Cazaméa-Catalan D, Besseau L, Falcón J, Magnanou E
PloS one9(12)e112380(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ercc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ercc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03470	NM_199785.1 Latest version!	Danio rerio excision repair cross-complementation group 4 (ercc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ercc4 ( NM_199785.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956079.1 Danio rerio excision repair cross-complementation group 4 (ercc4), mRNA.

Price & Availability↑

CloneID	ODa03470
Clone ID Related Accession (Same CDS sequence)	NM_199785.1
Accession Version	NM_199785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1567872000000
Organism	Danio rerio(zebrafish)
Product	DNA repair endonuclease XPF
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054895.1. ##Evidence-Data-START## Transcript exon combination :: BC054895.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAGTG CTCTGTTGGA GTATGAGACG GAGATGTTTC TGAGTGTGTT CAGCACAGAC 
GGGCTGTTGA TCACGGGTGA AGGACTCGGC ATTGACCGCC TTCTGCTGCA CTTCATGAAG 
GTGTATTCTG AGGAGGGCAG CTTGGTGCTC TTGCTGAACA CCAGCACTCC TGAACAGGAG 
TATTTGACGG AGCGGCTGCG AGCTGAAGGA GTCACTCATC TTCCTCAAAC TGTGACCAGT 
GATGTGCAGA GTGCTGACAG ATATAACGTG TACACTAAAG GAGGAGTGCT GTTTGTCACT 
AGCCGAATCC TAGTGGTGGA TTTCCTGACT GACAGAATCC CTGCTCACCT AGTAACAGGA 
ATCCTGGTGT ACCGGGCACA TAATATCATT GAGTCTTGTC AGGAGGCTTT CATTTTGAGA 
CTTTATCGGC AGAAAAACAA AACCGGCTTC ATCAAGGCCT TTACAGATAA AGCCACGTCC 
TTCTCTTCAG GATTCTGTAA AGTCGAGCGT GTCATGAGAA ACTTGTTTGT GAAGAAACTT 
TTTCTCTGGC CAAGATTCCA AGCATCAGTG AACGCAACGC TGGACAAACA CAAGCCAGAT 
GTGGTGGAGC TGCACGTGAC TCTGACTCCA GCAATGAGAG CCATTCAGAG CTCCATTCTG 
GACATCATGA ATGCCTGCCT GAAAGAGCTG AAGCGCTACA ACCCCACACT GGAAGCAGAA 
GACCTTTCTT TGGAGAACAG CCTGGGCACT GCCTTTGAGA AAACAATTCG TCATTACCTT 
GACCCACTGT GGCATCAGCT GGGGTCCAGG ACCAAATCTC TTGTTCAGGA CCTGAAGATC 
TTAAGAACCC TTCTTCTCTA CCTCACTCAG TACGACTGTG TCACCTTCCT CAATCTGCTG 
GAGTCTTTGA GAACCAGCCA GAAAAGTTTT GGTAGTAATT CAGGTTGGTT GTTCCTGGAC 
TCAAGCATGT CTATGTTTGT AAATGCTCGC AGTCGTGTGT ATCGCATTCA GGAGTCTAAG 
AAAAAACTGA AAGTTGGAGA GACTGAGCAG AAACAAAACC TAGCGCCTCC GAAAAGAGAG 
CTTGTGCTTG AGAAGAATCC AAAGTGGGAA GCACTTACAG AAGTCCTACA GGAGATTGAG 
AAAGAAAACA GCAAATCTGA GCACGAACCA GGCCGAGTGT TGATATGCGC CAGTGATGAC 
AGAACCTGTG CTCAGCTGAA GGAATACATC CAAAAAGGGC CAGAACACCT ACTGAACCGC 
CTTTATTCAC GCACTATTGG AAAAGAAGAT CCTCAGCCTG ATTTACTGAA GCTTGACAAA 
GGCTGGCAAA AGAAAGACTC TGCTAGGAGC AAAGGAAAAA ACAGCCGTCA GAAAAGAACC 
AAAGCCAACT CAAAGAAACC GGCAAAGATG GAGAAACTGT CAAGAGTCTC TCAGGACGAT 
AGTGCTGATA AGGACCTAAA TGAGAACAGT GAAGATGAAG GAGGGTTGAT TGAAGAGGAG 
GAAGAAGAGG AGATTGTTCT GGATCTATCA TCTGATGATT ATTATGGTAT TCTGAAAGAG 
CCACTGACTG TCATTCACCC ACTGAAGGGA TGCAGTGACC CCTACAGTCT GACCCGCGTG 
CTGCATGAGG TTGACCCCAT GTTTGTCGTC TTGTACGATG CTGAACTCAG TTTTGTGCGG 
CAGCTGGAGA TTTATAAAGC ATGCAGGCCT GGAAAACCAC TCCGTGTGTA TTTTCTCATA 
TATGGAGGCT CAACAGAAGA ACAGAGATAT CTTACAGCAT TAAACAAAGA AAAGCAAGCG 
TTTGAGCACC TCATAAGGGA AAAGGCCAGC ATGGTTGTGC CAGAAGAAAG AGAAGGTAGA 
GAAGACACCA ATCTAGATCT TGTCCGCAGT CAGGAACCTG CTAGTGCCGC CACAAACACC 
CGCAAAGCAG GGGGACTTGA AGAGGTTAAA GAGCCTCATC GGATCATCGT GGACATGAGA 
GAGTTTCGCA GTGAACTTCC TTCGCTTCTG CATCGACGTG GTCTGGACAT CGAACCCGTC 
ACCTTAGAGG TCGGCGACTA CATACTGACG TCTGACATCT GCGTAGAGCG CAAAAGCGTC 
AGCGACCTCA TTGGCTCCTT ACAGAGCGGA CGCCTTTACA CACAGTGCCT CTCCATGACT 
CGATTCTACC GCCGTCCTGT GTTGCTCATC GAGTTTGACC CCGCTAAACC ATTCTCCCTG 
GTGGCCCGCA GTGACTTTCG GCAGGAGATC TCAGCCAACG ATGTCACCTC CAAACTGACC 
CTTCTCACCC TCCATTTCCC CCGCCTTAGG CTCCTCTGGT GCCCTTCGCC GTATGTGACG 
GCAGAGCTGT TTCAGGAGCT CAAGCATGGC CGTACCGAAC CGGACGCCAG CACAGCTCAG 
GCAGTTACAG CGGAGTCAGA GACTGTGACC GAATCTGCAG ATCTGTACAA TCCTGGACCT 
TACGACTTCC TGCTGCGTAT GCCTGGAGTC AATGTGAAGA ACGTCAAGAG TCTGATAAAA 
CATGTTTCCT GCCTCGCTGA TCTTGTGACA TTTAGCCAGG AGACGCTCAG TGAGATTTTG 
GGCAGCAGCA GCAATGCCAG GATGCTTTAT GAATTCATAC ACAATCCTAT GGATGTGCGG 
CCCATTCAGA AGAATAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956079.1
CDS	204..2864
Translation	MASALLEYETEMFLSVFSTDGLLITGEGLGIDRLLLHFMKVYSEEGSLVLLLNTSTPEQEYLTERLRAEGVTHLPQTVTSDVQSADRYNVYTKGGVLFVTSRILVVDFLTDRIPAHLVTGILVYRAHNIIESCQEAFILRLYRQKNKTGFIKAFTDKATSFSSGFCKVERVMRNLFVKKLFLWPRFQASVNATLDKHKPDVVELHVTLTPAMRAIQSSILDIMNACLKELKRYNPTLEAEDLSLENSLGTAFEKTIRHYLDPLWHQLGSRTKSLVQDLKILRTLLLYLTQYDCVTFLNLLESLRTSQKSFGSNSGWLFLDSSMSMFVNARSRVYRIQESKKKLKVGETEQKQNLAPPKRELVLEKNPKWEALTEVLQEIEKENSKSEHEPGRVLICASDDRTCAQLKEYIQKGPEHLLNRLYSRTIGKEDPQPDLLKLDKGWQKKDSARSKGKNSRQKRTKANSKKPAKMEKLSRVSQDDSADKDLNENSEDEGGLIEEEEEEEIVLDLSSDDYYGILKEPLTVIHPLKGCSDPYSLTRVLHEVDPMFVVLYDAELSFVRQLEIYKACRPGKPLRVYFLIYGGSTEEQRYLTALNKEKQAFEHLIREKASMVVPEEREGREDTNLDLVRSQEPASAATNTRKAGGLEEVKEPHRIIVDMREFRSELPSLLHRRGLDIEPVTLEVGDYILTSDICVERKSVSDLIGSLQSGRLYTQCLSMTRFYRRPVLLIEFDPAKPFSLVARSDFRQEISANDVTSKLTLLTLHFPRLRLLWCPSPYVTAELFQELKHGRTEPDASTAQAVTAESETVTESADLYNPGPYDFLLRMPGVNVKNVKSLIKHVSCLADLVTFSQETLSEILGSSSNARMLYEFIHNPMDVRPIQKNK

Target ORF information:

RefSeq Version	NM_199785.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio excision repair cross-complementation group 4 (ercc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199785.1

ORF Insert Sequence:

ATGGCGAGTG CTCTGTTGGA GTATGAGACG GAGATGTTTC TGAGTGTGTT CAGCACAGAC 
GGGCTGTTGA TCACGGGTGA AGGACTCGGC ATTGACCGCC TTCTGCTGCA CTTCATGAAG 
GTGTATTCTG AGGAGGGCAG CTTGGTGCTC TTGCTGAACA CCAGCACTCC TGAACAGGAG 
TATTTGACGG AGCGGCTGCG AGCTGAAGGA GTCACTCATC TTCCTCAAAC TGTGACCAGT 
GATGTGCAGA GTGCTGACAG ATATAACGTG TACACTAAAG GAGGAGTGCT GTTTGTCACT 
AGCCGAATCC TAGTGGTGGA TTTCCTGACT GACAGAATCC CTGCTCACCT AGTAACAGGA 
ATCCTGGTGT ACCGGGCACA TAATATCATT GAGTCTTGTC AGGAGGCTTT CATTTTGAGA 
CTTTATCGGC AGAAAAACAA AACCGGCTTC ATCAAGGCCT TTACAGATAA AGCCACGTCC 
TTCTCTTCAG GATTCTGTAA AGTCGAGCGT GTCATGAGAA ACTTGTTTGT GAAGAAACTT 
TTTCTCTGGC CAAGATTCCA AGCATCAGTG AACGCAACGC TGGACAAACA CAAGCCAGAT 
GTGGTGGAGC TGCACGTGAC TCTGACTCCA GCAATGAGAG CCATTCAGAG CTCCATTCTG 
GACATCATGA ATGCCTGCCT GAAAGAGCTG AAGCGCTACA ACCCCACACT GGAAGCAGAA 
GACCTTTCTT TGGAGAACAG CCTGGGCACT GCCTTTGAGA AAACAATTCG TCATTACCTT 
GACCCACTGT GGCATCAGCT GGGGTCCAGG ACCAAATCTC TTGTTCAGGA CCTGAAGATC 
TTAAGAACCC TTCTTCTCTA CCTCACTCAG TACGACTGTG TCACCTTCCT CAATCTGCTG 
GAGTCTTTGA GAACCAGCCA GAAAAGTTTT GGTAGTAATT CAGGTTGGTT GTTCCTGGAC 
TCAAGCATGT CTATGTTTGT AAATGCTCGC AGTCGTGTGT ATCGCATTCA GGAGTCTAAG 
AAAAAACTGA AAGTTGGAGA GACTGAGCAG AAACAAAACC TAGCGCCTCC GAAAAGAGAG 
CTTGTGCTTG AGAAGAATCC AAAGTGGGAA GCACTTACAG AAGTCCTACA GGAGATTGAG 
AAAGAAAACA GCAAATCTGA GCACGAACCA GGCCGAGTGT TGATATGCGC CAGTGATGAC 
AGAACCTGTG CTCAGCTGAA GGAATACATC CAAAAAGGGC CAGAACACCT ACTGAACCGC 
CTTTATTCAC GCACTATTGG AAAAGAAGAT CCTCAGCCTG ATTTACTGAA GCTTGACAAA 
GGCTGGCAAA AGAAAGACTC TGCTAGGAGC AAAGGAAAAA ACAGCCGTCA GAAAAGAACC 
AAAGCCAACT CAAAGAAACC GGCAAAGATG GAGAAACTGT CAAGAGTCTC TCAGGACGAT 
AGTGCTGATA AGGACCTAAA TGAGAACAGT GAAGATGAAG GAGGGTTGAT TGAAGAGGAG 
GAAGAAGAGG AGATTGTTCT GGATCTATCA TCTGATGATT ATTATGGTAT TCTGAAAGAG 
CCACTGACTG TCATTCACCC ACTGAAGGGA TGCAGTGACC CCTACAGTCT GACCCGCGTG 
CTGCATGAGG TTGACCCCAT GTTTGTCGTC TTGTACGATG CTGAACTCAG TTTTGTGCGG 
CAGCTGGAGA TTTATAAAGC ATGCAGGCCT GGAAAACCAC TCCGTGTGTA TTTTCTCATA 
TATGGAGGCT CAACAGAAGA ACAGAGATAT CTTACAGCAT TAAACAAAGA AAAGCAAGCG 
TTTGAGCACC TCATAAGGGA AAAGGCCAGC ATGGTTGTGC CAGAAGAAAG AGAAGGTAGA 
GAAGACACCA ATCTAGATCT TGTCCGCAGT CAGGAACCTG CTAGTGCCGC CACAAACACC 
CGCAAAGCAG GGGGACTTGA AGAGGTTAAA GAGCCTCATC GGATCATCGT GGACATGAGA 
GAGTTTCGCA GTGAACTTCC TTCGCTTCTG CATCGACGTG GTCTGGACAT CGAACCCGTC 
ACCTTAGAGG TCGGCGACTA CATACTGACG TCTGACATCT GCGTAGAGCG CAAAAGCGTC 
AGCGACCTCA TTGGCTCCTT ACAGAGCGGA CGCCTTTACA CACAGTGCCT CTCCATGACT 
CGATTCTACC GCCGTCCTGT GTTGCTCATC GAGTTTGACC CCGCTAAACC ATTCTCCCTG 
GTGGCCCGCA GTGACTTTCG GCAGGAGATC TCAGCCAACG ATGTCACCTC CAAACTGACC 
CTTCTCACCC TCCATTTCCC CCGCCTTAGG CTCCTCTGGT GCCCTTCGCC GTATGTGACG 
GCAGAGCTGT TTCAGGAGCT CAAGCATGGC CGTACCGAAC CGGACGCCAG CACAGCTCAG 
GCAGTTACAG CGGAGTCAGA GACTGTGACC GAATCTGCAG ATCTGTACAA TCCTGGACCT 
TACGACTTCC TGCTGCGTAT GCCTGGAGTC AATGTGAAGA ACGTCAAGAG TCTGATAAAA 
CATGTTTCCT GCCTCGCTGA TCTTGTGACA TTTAGCCAGG AGACGCTCAG TGAGATTTTG 
GGCAGCAGCA GCAATGCCAG GATGCTTTAT GAATTCATAC ACAATCCTAT GGATGTGCGG 
CCCATTCAGA AGAATAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiplexed CRISPR/Cas9-mediated knockout of 19 Fanconi anemia pathway genes in zebrafish revealed their roles in growth, sexual development and fertility.
PLoS genetics14(12)e1007821(2018 12)
Ramanagoudr-Bhojappa R,Carrington B,Ramaswami M,Bishop K,Robbins GM,Jones M,Harper U,Frederickson SC,Kimble DC,Sood R,Chandrasekharappa SC

Mercury (II) impairs nucleotide excision repair (NER) in zebrafish (Danio rerio) embryos by targeting primarily at the stage of DNA incision.
Aquatic toxicology (Amsterdam, Netherlands)19297-104(2017 Nov)
Chang Y,Lee WY,Lin YJ,Hsu T

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The timing of Timezyme diversification in vertebrates.
PloS one9(12)e112380(2014)
Cazaméa-Catalan D,Besseau L,Falcón J,Magnanou E

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ercc4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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