col4a5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col4a5
Entrez Gene ID	323561
Full Name	collagen, type IV, alpha 5 (Alport syndrome)
Synonyms	col4a1,wu:fc02g11
General protein information	Preferred Names collagen, type IV, alpha 5 (Alport syndrome) Names collagen alpha-5(IV) chain collagen, type IV, alpha 1 dragnet drg type IV collagen alpha 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166502.2	XP_005166559.1	collagen alpha-5(IV) chain isoform X1
XM_005166502.3	XP_005166559.1	collagen alpha-5(IV) chain isoform X2
NM_001123230.1	NP_001116702.1	collagen alpha-5(IV) chain precursor
XM_021477401.1	XP_021333076.1	collagen alpha-5(IV) chain isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col4a5	XP_343779.2
Danio rerio (zebrafish)	col4a5	NP_001116702.1
Xenopus tropicalis (tropical clawed frog)	col4a5	XP_002931863.2

Gene Ontology
Bibliography

Related articles in PubMed

Fishing for collagen function: About development, regeneration and disease.
Bretaud S, Nauroy P, Malbouyres M, Ruggiero F
Seminars in cell & developmental biology89100-108(2019 05)

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Xue Y, Liu D, Cui G, Ding Y, Ai D, Gao S, Zhang Y, Suo S, Wang X, Lv P, Zhou C, Li Y, Chen X, Peng G, Jing N, Han JJ, Liu F
Cell reports27(5)1567-1578.e5(2019 04)

Testicular transcriptome alterations in zebrafish (Danio rerio) exposure to 17β-estradiol.
Zheng Y, Yuan J, Meng S, Chen J, Gu Z
Chemosphere21814-25(2019 Mar)

Identification of p53-target genes in Danio rerio.
Mandriani B, Castellana S, Rinaldi C, Manzoni M, Venuto S, Rodriguez-Aznar E, Galceran J, Nieto MA, Borsani G, Monti E, Mazza T, Merla G, Micale L
Scientific reports632474(2016 09)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following col4a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col4a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37041	NM_001123230.1 Latest version!	Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa13419	XM_005166502.3 Latest version!	Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa143202	XM_021477401.1 Latest version!	Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa13419	XM_005166502.2	Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col4a5 ( NM_001123230.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116702.1 Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), mRNA.

Price & Availability↑

CloneID	ODa37041
Clone ID Related Accession (Same CDS sequence)	NM_001123230.1
Accession Version	NM_001123230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574438400000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-5(IV) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR936978.8 and CR354588.9. On Apr 11, 2008 this sequence version replaced XM_001334467.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA4476796, SAMEA898401 [ECO:0000350] ##Evidence-Data-END##

ATGAACTTAA AAAGTCTCGG CGCGGTGTTG CTGCTTGTGC TCACTCTGAG TGCGGGAGTA 
GAGAAATCCG AGAGTGCGGC ATGTCATGGT TGTGGGACTG GATCAAAATG TGACTGCAGT 
TCAGTGAAAG GAGTGAAGGG AGATCGAGGA CTTCCGGGTT TAAAGGGTCA GCCGGGTGTT 
CCAGGTTTCC CAGGACCAGA GGGACCAATG GGACCTCGTG GAGAGAAAGG TGATGACGGA 
CCTCAAGGGT CGATAGGCCC CAAAGGAATA AGAGGACCTC CAGGATTACC TGGATTTTCA 
GGAACACCAG GAATCCCAGG TCTTCCAGGA CAGGATGGTG CTCCAGGACC CAGAGGAATT 
CCAGGCTGTA ATGGAACTAA AGGTGAAAGA GGATTCCCTG GAAATCCAGG TCGCTCAGGT 
TTAGATGGTC AACCAGGTCC ACCAGGTCTT ATAGGACAAA AAGCAAAACC TGGGGAAGTC 
ATCGACATTA ACCGCATTTC AGAAAATGGA CTTCCAGGCT TTAATGGCTT TACAGGCAGA 
AGGGGTGATC CCGGACCCCC AGGTCTTCAA GGGCCTATCG GTCCTCCTGG TCCAAAAGGG 
TTTGAGGGTC GCCCAGGCCC TCCTGGTCCA CCGGGACCAA AAGGAAACAT GGGATTGAAT 
TTCCAAGGAC CCAAAGGAGA GAAGGGTGAC CGTGGATTAA AAGGACCTCC AGGGCCACCA 
GGACAAGTTG GTGAAGTGTC AGGTTCACCA GATACAGTCA TTCAACAAGG AGAAAAGGGT 
GATCGTGGCA TACCTGGAGA TACTGGTTTC CCAGGGCCCC CTGGACCCCC TGGTCCACAA 
GGAGGTCAGA AAGGAGAGAA AGGAGAACCT GGGGAGGCTG GAAAGAGGGG AAAACCTGGC 
AAAGATGGTG AACCAGGTCT TCTTGGATAT CCGGGTGATA AAGGAGACCA AGGGCCTCCT 
GGTCTGCCTG GACGAGATGG TTTTAGAGGT GAGAAAGGTG ATCAAGGGCC ACCTGGACCT 
CCCTCCTACC GGGGTGATCA GTTCACAGGT GTAGGTGGAA TAGGACCAAA AGGAGACCGT 
GGTGATCCAG GACCCTCTGG TATTAAAGGA GACAGAGGAC TACCAGGTAT TCAGGGGCCA 
CCTGGTCCAC AGGGTCCTCC AGGAATTGGA GGTCCAGGAC CTCCCGGTTC ACAAGGATTG 
CCTGGAGAAC GAGGAGAAAA GGGTGATGAG GGACAGCCTG GAATCTCACT TCCTGGACCA 
CCCGGGAGAC CCGGACCAAC AGGACCACAA GGAATACAAG GACCACCTGG CCCCCCAGGA 
CCATTCAACA GTGATGTAGA ATGTAATCGT GGTCTTCCTG GTCCTCCAGG CCTGCAGGGG 
GAGCGTGGGT TTACTGGAGA TAGTGGGCAG AAAGGTCAAA AAGGCGAGAC TTGTGTGAAT 
TGTTTCTCCA ATGGAAACCC TGTCCCAGGA CCCCAAGGTT TTCCAGGTCC TCCTGGAAGT 
CCAGGTGCTC CTGGGGCGTC TGGATCTAAA GGAGACAGGG GATATCCGGG AGGACCAGGG 
CTTATGGGCC CACCTGGAAT ACCTGGACCT CAGGGTTCCC CTGGACTCCC TGGAGAGAAG 
GGTGACCCAG GCGATACCCT CTCTGTTCTT GGCGTGAAGG GAGATAAAGG AGACACAGGT 
TTTCCTGGCT CTCCAGGTCT GCCGGGACTC GAAGGAAGAC CTGGACGTGA TGGAGTGCCT 
GGTCCACCTG GCCTCAGAGG ACCACCTGGT TTGGGACTGA AAGGTACACA AGGTCCCAAC 
GGTGAACCTG GTTTGCCAGG ACCTCCTGGA GACAGAGGTT TACCAGGTTC CCCAGGATTT 
GGTCCTCAAG GACCTTCTGG AGAAAAAGGT ATTCAGGGTG TTTCAGGAAG ACCAGGGGCA 
CCTGGACTAC CAGGTCAGAA AGGTGAACCT GGGATGACTG TGCCAGAAGT TGGTGAACGT 
GGACCCCCAG GACAGGATGG AGATCCTGGT TCACAAGGAC GTCCAGGTGA TTCAGGCCCA 
CCTGGACAGC CTGGGTTCCC AGGTTTGCCT GGATCCAAAG GAGAGCCTGG TCTTCCTGGT 
ATTGGTTTGC CAGGACCTCC TGGTGCTAAA GGATTTCCTG GTATTGCTGG TTCCCCTGGT 
GGACCTGGCA TTCCTGGACG ACCAGGTTTA GATGGACTGC CTGGTCAGCC TGGATTACCT 
GGATCCAAAG GAGATCCTGG TTATGGTCTG CCTGGTCCTC CAGGTCCCAC AGGATCACCA 
GGCATTAAAG GTGGACCCGG TCCGAAAGGT GATTCTGGTT TCCCTGGAAG CCCTGGTCAA 
CCAGGCCGCC CAGGTCTCGA TGGGGCTCCT GGACCTAAAG GTGATGCTGG TTTTCCTGGT 
GGTCCTGGTC CTCGTGGCCC CCCTGGGGCT CCTGCTTTTG GTCTTCAGGG CCCTCCTGGT 
CCTCCTGGAG CTCCAGGCTC TATTGGATCT CCTGGAGTAC CGGGGGCGAA TGGAGAAAAA 
GGAGACAGAG GCCCTCCAGG TCTGAGTACT CCAGGCTTCC AGGGTGATAG AGGAATTTCT 
GGACTTCCTG GACCTCCTGG TCCAGTTGGT CCACCTGGAG TTCCAGGCCG ACCTGGACAA 
GATGGCCTAC CAGGACTCCC TGGATCTAAG GGAGAAATGG GCTCAATGGG GCCTCCCGGA 
TCAAAAGGGA ACCCCGGCAA CCCTGGAGCT CCCGGCTTTC CAGGACCAAA AGGAGATGAT 
GGTGTCCCAG GACAGTCAGG AAATCCAGGT TTTCCAGGGT TAAAAGGAGA CAAAGGTGAA 
ACAGGCATTC AAGGCCCGCC TGGCATAACC ACATCTACAA GTGTACTGAA AGGACAAAAA 
GGAGAATCTG GTGTTCCAGG TTTCAGTGGA CCGCCAGGAC CAAAGGGTAT TACAGGTATC 
TCTGGAGATC CTGGTCTGCC TGGGCAAGAT GGACGACCAG GACTACCAGG ACCCCCAGGC 
CCCAAAGGTG ATGCTGGTCT CCCAGGAATT CCAGGTTCTC CAGGAGCCAC AGGATTAAAG 
GGGGCCATGG GAGAGATGGG ATTGCCAGGA GTTCCTGGAG TCAAAGGAAA TTCCGGCCAA 
CCTGGCCAAC CTGGCCAGCC AGGATCCCCA GGTGGACCAG GATTCCCAGG ACCTAAAGGT 
GAAGCTGGTG TACCAGGATA CGGATTACCT GGAAATCCCG GACCTAAGGG TGAACCAGGC 
ACTCCAGGCT TTCCTGGACC CGCTGGCTTG AAAGGCCAGC CTGGCTTTCC AGGACCTCAA 
GGCTTGACTG GTAGCCCTGG ACCAAAGGGT GATCCGGGAC TCCCAGGATT TCAAGGTGCA 
CCTGGCAATC CTGGCCCAAA AGGTTTGGAT GGTGCTCCAG GCCCTGCAGG TTTAAATGGA 
GCACCTGGTA GGCCAGGAGA TCCCGGACGA TCTGGATTAC CTGGTATCCC TGGAGATAAA 
GGTCTTGCTG GTCGTGATGG TATTCCAGGA CCAGCAGGTC AAAAGGGTGA ACCAGGCTTG 
CCTGGTCAAC CTGGTTTGAA AGGCGCTAAA GGAGATCCTG GTTTTCCGGG TCCTGCCGGT 
AGTGCTGGAT TTCCTGGTCC AAAGGGTGAT GCAGGATTTC CTGGCTTGCC TGGTCAGATT 
GGTTCTGTAG GACCACCTGG GCCTCCAGGC TTGCCACTGC AGGGACCTAA AGGTATCCAA 
GGTCCACCTG GTCCCCCCGG AAGAGCTGGT AGCGGAGGAG TGAAGGGTGA GAAGGGTTTG 
CCTGGAGTTC CTGGGACGCC TGGAGAAGCA GGACAGAAAG GAGATTATGG TTCTCCTGGG 
TTTCCGGGCT CCCCAGGGAG TCCAGGACCA AATGGCCTCA AAGGAGATCC TGGACCTCAA 
GGTGTTCCTG GTTTTCCAGG ATCTAAAGGA GTGTCTGGTC CACCTGGTAT TTCTGGCCTC 
CCTGGAGCAC GTGGTGATCC TGGAGATAAA GGACCTCCCG GACCCCCAGG TTATGCTGCA 
GAGCCTATTG TCTTGAAGGG CGAGCGTGGC CCACCAGGAC CTCCAGGTAA TCCAGGCGGC 
AGAGGACAAG CCGGACCCAT CGGATTGCAG GGACTCCCAG GTTCGCCTGG CAGCTCTGGA 
GACCCAGGAC CAATGGGACC TCCAGGATTT AATGGCCCAC CTGGAAGGAA GGGAGACACA 
GGTCCAGCTG GACAGCCAGG TCAGCGTGGT TACCCCGGCC CTCCAGGTCC AGATGGGGCT 
CCGGGGCCTC CAGGTATCAC GGGAACTTTG TCTGGTGCAC ACGGTTTTCT GATCACACGC 
CACAGTCAGA CCATAGAAGT GCCATCGTGT CCTGATGGCA CAAGCCCAAT CTACGAGGGC 
TACTCTTTAC TCTACGTGCA AGGCAACGAG CGGGCACACG GTCAAGATCT GGGTACGGCT 
GGCAGCTGTC TACGGCGTTT CAGCACGATG CCTTTCATGT TCTGCAACAT CAACAATGTC 
TGTAACTTCG CTTCCCGTAA TGATTACTCA TACTGGCTGT CCACACCACA GCCCATGCCC 
ATGACCATGG CACCCATCAC TGGAGAGAAC ATCAAACCTT ACATCAGCCG GTGTTCTGTG 
TGTGAAGCTC CAGCAATGGT GATTGCTGTA CACAGTCAGA CCATTCAGTT GCCAGCGTGC 
CCTGAAAACT GGGATACCCT CTGGATCGGT TACTCCTTCA TGATGCACAC CAGTGCAGGT 
GCTGAGGGTT CAGGTCAGGC TCTGGCCTCT CCGGGCTCCT GTCTGGAGGA GTTTCGCTCC 
GCTCCCTTCA TTGAGTGCCA CGGACGAGGA ACCTGCAATT ATTACGGCAA CTCCTACAGC 
TTCTGGCTGG CCACTGTGGA CGTGAACGAA ATGTTCAGAA AGCCGCAGTC AGAGACTCTA 
AAAGCTGGAA ACTTGAGGAC CCGCGTGAGC CGCTGTGTGG TGTGTATGAA GAGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116702.1
CDS	1..4980
Translation	MNLKSLGAVLLLVLTLSAGVEKSESAACHGCGTGSKCDCSSVKGVKGDRGLPGLKGQPGVPGFPGPEGPMGPRGEKGDDGPQGSIGPKGIRGPPGLPGFSGTPGIPGLPGQDGAPGPRGIPGCNGTKGERGFPGNPGRSGLDGQPGPPGLIGQKAKPGEVIDINRISENGLPGFNGFTGRRGDPGPPGLQGPIGPPGPKGFEGRPGPPGPPGPKGNMGLNFQGPKGEKGDRGLKGPPGPPGQVGEVSGSPDTVIQQGEKGDRGIPGDTGFPGPPGPPGPQGGQKGEKGEPGEAGKRGKPGKDGEPGLLGYPGDKGDQGPPGLPGRDGFRGEKGDQGPPGPPSYRGDQFTGVGGIGPKGDRGDPGPSGIKGDRGLPGIQGPPGPQGPPGIGGPGPPGSQGLPGERGEKGDEGQPGISLPGPPGRPGPTGPQGIQGPPGPPGPFNSDVECNRGLPGPPGLQGERGFTGDSGQKGQKGETCVNCFSNGNPVPGPQGFPGPPGSPGAPGASGSKGDRGYPGGPGLMGPPGIPGPQGSPGLPGEKGDPGDTLSVLGVKGDKGDTGFPGSPGLPGLEGRPGRDGVPGPPGLRGPPGLGLKGTQGPNGEPGLPGPPGDRGLPGSPGFGPQGPSGEKGIQGVSGRPGAPGLPGQKGEPGMTVPEVGERGPPGQDGDPGSQGRPGDSGPPGQPGFPGLPGSKGEPGLPGIGLPGPPGAKGFPGIAGSPGGPGIPGRPGLDGLPGQPGLPGSKGDPGYGLPGPPGPTGSPGIKGGPGPKGDSGFPGSPGQPGRPGLDGAPGPKGDAGFPGGPGPRGPPGAPAFGLQGPPGPPGAPGSIGSPGVPGANGEKGDRGPPGLSTPGFQGDRGISGLPGPPGPVGPPGVPGRPGQDGLPGLPGSKGEMGSMGPPGSKGNPGNPGAPGFPGPKGDDGVPGQSGNPGFPGLKGDKGETGIQGPPGITTSTSVLKGQKGESGVPGFSGPPGPKGITGISGDPGLPGQDGRPGLPGPPGPKGDAGLPGIPGSPGATGLKGAMGEMGLPGVPGVKGNSGQPGQPGQPGSPGGPGFPGPKGEAGVPGYGLPGNPGPKGEPGTPGFPGPAGLKGQPGFPGPQGLTGSPGPKGDPGLPGFQGAPGNPGPKGLDGAPGPAGLNGAPGRPGDPGRSGLPGIPGDKGLAGRDGIPGPAGQKGEPGLPGQPGLKGAKGDPGFPGPAGSAGFPGPKGDAGFPGLPGQIGSVGPPGPPGLPLQGPKGIQGPPGPPGRAGSGGVKGEKGLPGVPGTPGEAGQKGDYGSPGFPGSPGSPGPNGLKGDPGPQGVPGFPGSKGVSGPPGISGLPGARGDPGDKGPPGPPGYAAEPIVLKGERGPPGPPGNPGGRGQAGPIGLQGLPGSPGSSGDPGPMGPPGFNGPPGRKGDTGPAGQPGQRGYPGPPGPDGAPGPPGITGTLSGAHGFLITRHSQTIEVPSCPDGTSPIYEGYSLLYVQGNERAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPQPMPMTMAPITGENIKPYISRCSVCEAPAMVIAVHSQTIQLPACPENWDTLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYGNSYSFWLATVDVNEMFRKPQSETLKAGNLRTRVSRCVVCMKRT

Target ORF information:

RefSeq Version	NM_001123230.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123230.1

ORF Insert Sequence:

ATGAACTTAA AAAGTCTCGG CGCGGTGTTG CTGCTTGTGC TCACTCTGAG TGCGGGAGTA 
GAGAAATCCG AGAGTGCGGC ATGTCATGGT TGTGGGACTG GATCAAAATG TGACTGCAGT 
TCAGTGAAAG GAGTGAAGGG AGATCGAGGA CTTCCGGGTT TAAAGGGTCA GCCGGGTGTT 
CCAGGTTTCC CAGGACCAGA GGGACCAATG GGACCTCGTG GAGAGAAAGG TGATGACGGA 
CCTCAAGGGT CGATAGGCCC CAAAGGAATA AGAGGACCTC CAGGATTACC TGGATTTTCA 
GGAACACCAG GAATCCCAGG TCTTCCAGGA CAGGATGGTG CTCCAGGACC CAGAGGAATT 
CCAGGCTGTA ATGGAACTAA AGGTGAAAGA GGATTCCCTG GAAATCCAGG TCGCTCAGGT 
TTAGATGGTC AACCAGGTCC ACCAGGTCTT ATAGGACAAA AAGCAAAACC TGGGGAAGTC 
ATCGACATTA ACCGCATTTC AGAAAATGGA CTTCCAGGCT TTAATGGCTT TACAGGCAGA 
AGGGGTGATC CCGGACCCCC AGGTCTTCAA GGGCCTATCG GTCCTCCTGG TCCAAAAGGG 
TTTGAGGGTC GCCCAGGCCC TCCTGGTCCA CCGGGACCAA AAGGAAACAT GGGATTGAAT 
TTCCAAGGAC CCAAAGGAGA GAAGGGTGAC CGTGGATTAA AAGGACCTCC AGGGCCACCA 
GGACAAGTTG GTGAAGTGTC AGGTTCACCA GATACAGTCA TTCAACAAGG AGAAAAGGGT 
GATCGTGGCA TACCTGGAGA TACTGGTTTC CCAGGGCCCC CTGGACCCCC TGGTCCACAA 
GGAGGTCAGA AAGGAGAGAA AGGAGAACCT GGGGAGGCTG GAAAGAGGGG AAAACCTGGC 
AAAGATGGTG AACCAGGTCT TCTTGGATAT CCGGGTGATA AAGGAGACCA AGGGCCTCCT 
GGTCTGCCTG GACGAGATGG TTTTAGAGGT GAGAAAGGTG ATCAAGGGCC ACCTGGACCT 
CCCTCCTACC GGGGTGATCA GTTCACAGGT GTAGGTGGAA TAGGACCAAA AGGAGACCGT 
GGTGATCCAG GACCCTCTGG TATTAAAGGA GACAGAGGAC TACCAGGTAT TCAGGGGCCA 
CCTGGTCCAC AGGGTCCTCC AGGAATTGGA GGTCCAGGAC CTCCCGGTTC ACAAGGATTG 
CCTGGAGAAC GAGGAGAAAA GGGTGATGAG GGACAGCCTG GAATCTCACT TCCTGGACCA 
CCCGGGAGAC CCGGACCAAC AGGACCACAA GGAATACAAG GACCACCTGG CCCCCCAGGA 
CCATTCAACA GTGATGTAGA ATGTAATCGT GGTCTTCCTG GTCCTCCAGG CCTGCAGGGG 
GAGCGTGGGT TTACTGGAGA TAGTGGGCAG AAAGGTCAAA AAGGCGAGAC TTGTGTGAAT 
TGTTTCTCCA ATGGAAACCC TGTCCCAGGA CCCCAAGGTT TTCCAGGTCC TCCTGGAAGT 
CCAGGTGCTC CTGGGGCGTC TGGATCTAAA GGAGACAGGG GATATCCGGG AGGACCAGGG 
CTTATGGGCC CACCTGGAAT ACCTGGACCT CAGGGTTCCC CTGGACTCCC TGGAGAGAAG 
GGTGACCCAG GCGATACCCT CTCTGTTCTT GGCGTGAAGG GAGATAAAGG AGACACAGGT 
TTTCCTGGCT CTCCAGGTCT GCCGGGACTC GAAGGAAGAC CTGGACGTGA TGGAGTGCCT 
GGTCCACCTG GCCTCAGAGG ACCACCTGGT TTGGGACTGA AAGGTACACA AGGTCCCAAC 
GGTGAACCTG GTTTGCCAGG ACCTCCTGGA GACAGAGGTT TACCAGGTTC CCCAGGATTT 
GGTCCTCAAG GACCTTCTGG AGAAAAAGGT ATTCAGGGTG TTTCAGGAAG ACCAGGGGCA 
CCTGGACTAC CAGGTCAGAA AGGTGAACCT GGGATGACTG TGCCAGAAGT TGGTGAACGT 
GGACCCCCAG GACAGGATGG AGATCCTGGT TCACAAGGAC GTCCAGGTGA TTCAGGCCCA 
CCTGGACAGC CTGGGTTCCC AGGTTTGCCT GGATCCAAAG GAGAGCCTGG TCTTCCTGGT 
ATTGGTTTGC CAGGACCTCC TGGTGCTAAA GGATTTCCTG GTATTGCTGG TTCCCCTGGT 
GGACCTGGCA TTCCTGGACG ACCAGGTTTA GATGGACTGC CTGGTCAGCC TGGATTACCT 
GGATCCAAAG GAGATCCTGG TTATGGTCTG CCTGGTCCTC CAGGTCCCAC AGGATCACCA 
GGCATTAAAG GTGGACCCGG TCCGAAAGGT GATTCTGGTT TCCCTGGAAG CCCTGGTCAA 
CCAGGCCGCC CAGGTCTCGA TGGGGCTCCT GGACCTAAAG GTGATGCTGG TTTTCCTGGT 
GGTCCTGGTC CTCGTGGCCC CCCTGGGGCT CCTGCTTTTG GTCTTCAGGG CCCTCCTGGT 
CCTCCTGGAG CTCCAGGCTC TATTGGATCT CCTGGAGTAC CGGGGGCGAA TGGAGAAAAA 
GGAGACAGAG GCCCTCCAGG TCTGAGTACT CCAGGCTTCC AGGGTGATAG AGGAATTTCT 
GGACTTCCTG GACCTCCTGG TCCAGTTGGT CCACCTGGAG TTCCAGGCCG ACCTGGACAA 
GATGGCCTAC CAGGACTCCC TGGATCTAAG GGAGAAATGG GCTCAATGGG GCCTCCCGGA 
TCAAAAGGGA ACCCCGGCAA CCCTGGAGCT CCCGGCTTTC CAGGACCAAA AGGAGATGAT 
GGTGTCCCAG GACAGTCAGG AAATCCAGGT TTTCCAGGGT TAAAAGGAGA CAAAGGTGAA 
ACAGGCATTC AAGGCCCGCC TGGCATAACC ACATCTACAA GTGTACTGAA AGGACAAAAA 
GGAGAATCTG GTGTTCCAGG TTTCAGTGGA CCGCCAGGAC CAAAGGGTAT TACAGGTATC 
TCTGGAGATC CTGGTCTGCC TGGGCAAGAT GGACGACCAG GACTACCAGG ACCCCCAGGC 
CCCAAAGGTG ATGCTGGTCT CCCAGGAATT CCAGGTTCTC CAGGAGCCAC AGGATTAAAG 
GGGGCCATGG GAGAGATGGG ATTGCCAGGA GTTCCTGGAG TCAAAGGAAA TTCCGGCCAA 
CCTGGCCAAC CTGGCCAGCC AGGATCCCCA GGTGGACCAG GATTCCCAGG ACCTAAAGGT 
GAAGCTGGTG TACCAGGATA CGGATTACCT GGAAATCCCG GACCTAAGGG TGAACCAGGC 
ACTCCAGGCT TTCCTGGACC CGCTGGCTTG AAAGGCCAGC CTGGCTTTCC AGGACCTCAA 
GGCTTGACTG GTAGCCCTGG ACCAAAGGGT GATCCGGGAC TCCCAGGATT TCAAGGTGCA 
CCTGGCAATC CTGGCCCAAA AGGTTTGGAT GGTGCTCCAG GCCCTGCAGG TTTAAATGGA 
GCACCTGGTA GGCCAGGAGA TCCCGGACGA TCTGGATTAC CTGGTATCCC TGGAGATAAA 
GGTCTTGCTG GTCGTGATGG TATTCCAGGA CCAGCAGGTC AAAAGGGTGA ACCAGGCTTG 
CCTGGTCAAC CTGGTTTGAA AGGCGCTAAA GGAGATCCTG GTTTTCCGGG TCCTGCCGGT 
AGTGCTGGAT TTCCTGGTCC AAAGGGTGAT GCAGGATTTC CTGGCTTGCC TGGTCAGATT 
GGTTCTGTAG GACCACCTGG GCCTCCAGGC TTGCCACTGC AGGGACCTAA AGGTATCCAA 
GGTCCACCTG GTCCCCCCGG AAGAGCTGGT AGCGGAGGAG TGAAGGGTGA GAAGGGTTTG 
CCTGGAGTTC CTGGGACGCC TGGAGAAGCA GGACAGAAAG GAGATTATGG TTCTCCTGGG 
TTTCCGGGCT CCCCAGGGAG TCCAGGACCA AATGGCCTCA AAGGAGATCC TGGACCTCAA 
GGTGTTCCTG GTTTTCCAGG ATCTAAAGGA GTGTCTGGTC CACCTGGTAT TTCTGGCCTC 
CCTGGAGCAC GTGGTGATCC TGGAGATAAA GGACCTCCCG GACCCCCAGG TTATGCTGCA 
GAGCCTATTG TCTTGAAGGG CGAGCGTGGC CCACCAGGAC CTCCAGGTAA TCCAGGCGGC 
AGAGGACAAG CCGGACCCAT CGGATTGCAG GGACTCCCAG GTTCGCCTGG CAGCTCTGGA 
GACCCAGGAC CAATGGGACC TCCAGGATTT AATGGCCCAC CTGGAAGGAA GGGAGACACA 
GGTCCAGCTG GACAGCCAGG TCAGCGTGGT TACCCCGGCC CTCCAGGTCC AGATGGGGCT 
CCGGGGCCTC CAGGTATCAC GGGAACTTTG TCTGGTGCAC ACGGTTTTCT GATCACACGC 
CACAGTCAGA CCATAGAAGT GCCATCGTGT CCTGATGGCA CAAGCCCAAT CTACGAGGGC 
TACTCTTTAC TCTACGTGCA AGGCAACGAG CGGGCACACG GTCAAGATCT GGGTACGGCT 
GGCAGCTGTC TACGGCGTTT CAGCACGATG CCTTTCATGT TCTGCAACAT CAACAATGTC 
TGTAACTTCG CTTCCCGTAA TGATTACTCA TACTGGCTGT CCACACCACA GCCCATGCCC 
ATGACCATGG CACCCATCAC TGGAGAGAAC ATCAAACCTT ACATCAGCCG GTGTTCTGTG 
TGTGAAGCTC CAGCAATGGT GATTGCTGTA CACAGTCAGA CCATTCAGTT GCCAGCGTGC 
CCTGAAAACT GGGATACCCT CTGGATCGGT TACTCCTTCA TGATGCACAC CAGTGCAGGT 
GCTGAGGGTT CAGGTCAGGC TCTGGCCTCT CCGGGCTCCT GTCTGGAGGA GTTTCGCTCC 
GCTCCCTTCA TTGAGTGCCA CGGACGAGGA ACCTGCAATT ATTACGGCAA CTCCTACAGC 
TTCTGGCTGG CCACTGTGGA CGTGAACGAA ATGTTCAGAA AGCCGCAGTC AGAGACTCTA 
AAAGCTGGAA ACTTGAGGAC CCGCGTGAGC CGCTGTGTGG TGTGTATGAA GAGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a5 ( XM_005166502.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166559.1 Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), transcript variant X1, mRNA.

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CloneID	ODa13419
Clone ID Related Accession (Same CDS sequence)	XM_005166502.2 , XM_005166502.3
Accession Version	XM_005166502.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-5(IV) chain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005166502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTAA AAAGTCTCGG CGCGGTGTTG CTGCTTGTGC TCACTCTGAG TGCGGGAGTA 
GAGAAATCCG AGAGTGCGGC ATGTCATGGT TGTGGGACTG GATCAAAATG TGACTGCAGT 
TCAGTGAAAG GAGTGAAGGG AGATCGAGGA CTTCCGGGTT TAAAGGGTCA GCCGGGTGTT 
CCAGGTTTCC CAGGACCAGA GGGACCAATG GGACCTCGTG GAGAGAAAGG TGATGACGGA 
CCTCAAGGGT CGATAGGCCC CAAAGGAATA AGAGGACCTC CAGGATTACC TGGATTTTCA 
GGAACACCAG GAATCCCAGG TCTTCCAGGA CAGGATGGTG CTCCAGGACC CAGAGGAATT 
CCAGGCTGTA ATGGAACTAA AGGTGAAAGA GGATTCCCTG GAAATCCAGG TCGCTCAGGT 
TTAGATGGTC AACCAGGTCC ACCAGGTCTT ATAGGACAAA AAGGAGAACC TGGGGAAGTC 
ATCGACATTA ACCGCATTTC AGAAAATGGA CTTCCAGGCT TTAATGGCTT TACAGGCAGA 
AGGGGTGATC CCGGACCCCC AGGTCTTCAA GGGCCTATCG GTCCTCCTGG TCCAAAAGGG 
TTTGAGGGTC GCCCAGGCCC TCCTGGTCCA CCGGGACCAA AAGGAAACAT GGGATTGAAT 
TTCCAAGGAC CCAAAGGAGA GAAGGGTGAC CGTGGATTAA AAGGACCTCC AGGGCCACCA 
GGACAAGTTG GTGAAGTGTC AGGTTCACCA GATACAGTCA TTCAACAAGG AGAAAAGGGT 
GATCGTGGCA TACCTGGAGA TACTGGTTTC CCAGGGCCCC CTGGACCCCC TGGTCCACAA 
GGAGGTCAGA AAGGAGAGAA AGGAGAACCT GGGGAGGCTG GAAAGAGGGG AAAACCTGGC 
AAAGATGGTG AACCAGGTCT TCTTGGATAT CCGGGTGATA AAGGAGACCA AGGGCCTCCT 
GGTCTGCCTG GACGAGATGG TTTTAGAGGT GAGAAAGGTG ATCAAGGGCC ACCTGGACCT 
CCCTCCTACC GGGGTGATCA GTTCACAGGT GTAGGTGGAA TAGGACCAAA AGGAGACCGT 
GGTGATCCAG GACCCTCTGG TATTAAAGGA GACAGAGGAC TACCAGGTAT TCAGGGGCCA 
CCTGGTCCAC AGGGTCCTCC AGGAATTGGA GGTCCAGGAC CTCCCGGTTC ACAAGGATTG 
CCTGGAGAAC GAGGAGAAAA GGGTGATGAG GGACAGCCTG GAATCTCACT TCCTGGACCA 
CCCGGGAGAC CCGGACCAAC AGGACCACAA GGAATACAAG GACCACCTGG CCCCCCAGGA 
CCATTCAACA GTGATGTAGA ATGTAATCGT GGTCTTCCTG GTCCTCCAGG CCTGCAGGGG 
GAGCGTGGGT TTACTGGAGA TAGTGGGCAG AAAGGTCAAA AAGGCGAGAC TTGTGTGAAT 
TGTTTCTCCA ATGGAAACCC TGTCCCAGGA CCCCAAGGTT TTCCAGGTCC TCCTGGAAGT 
CCAGGTGCTC CTGGGGCGTC TGGATCTAAA GGAGACAGGG GATATCCGGG AGGACCAGGG 
CTTATGGGCC CACCTGGAAT ACCTGGACCT CAGGGTTCCC CTGGACTCCC TGGAGAGAAG 
GGTGACCCAG GCGATACCCT CTCTGTTCTT GGCGTGAAGG GAGATAAAGG AGACACAGGT 
TTTCCTGGCT CTCCAGGTCT GCCGGGACTC GAAGGAAGAC CTGGACGTGA TGGAGTGCCT 
GGTCCACCTG GCCTCAGAGG ACCACCTGGT TTGGGACTGA AAGGTACACA AGGTCCCAAC 
GGTGAACCTG GTTTGCCAGG ACCTCCTGGA GACAGAGGTT TACCAGGTTC CCCAGGATTT 
GGTCCTCAAG GACCTTCTGG AGAAAAAGGT ATTCAGGGTG TTTCAGGAAG ACCAGGGGCA 
CCTGGACTAC CAGGTCAGAA AGGTGAACCT GGGATGACTG TGCCAGAAGT TGGTGAACGT 
GGACCCCCAG GACAGGATGG AGATCCTGGT TCACAAGGAC GTCCAGGTGA TTCAGGCCCA 
CCTGGACAGC CTGGGTTCCC AGGTTTGCCT GGATCCAAAG GAGAGCCTGG TCTTCCTGGT 
ATTGGTTTGC CAGGACCTCC TGGTGCTAAA GGATTTCCTG GTATTGCTGG TTCCCCTGGT 
GGACCTGGCA TTCCTGGACG ACCAGGTTTA GATGGACTGC CTGGTCAGCC TGGATTACCT 
GGATCCAAAG GAGATCCTGG TTATGGTCTG CCTGGTCCTC CAGGTCCCAC AGGATCACCA 
GGCATTAAAG GTGGACCCGG TCCGAAAGGT GATTCTGGTT TCCCTGGAAG CCCTGGTCAA 
CCAGGCCGCC CAGGTCTCGA TGGGGCTCCT GGACCTAAAG GTGATGCTGG TTTTCCTGGT 
GGTCCTGGTC CTCGTGGCCC CCCTGGGGCT CCTGCTTTTG GTCTTCAGGG CCCTCCTGGT 
CCTCCTGGAG CTCCAGGCTC TATTGGATCT CCTGGAGTAC CGGGGGCGAA TGGAGAAAAA 
GGAGACAGAG GCCCTCCAGG TCTGAGTACT CCAGGCTTCC AGGGTGATAG AGGAATTTCT 
GGACTTCCTG GACCTCCTGG TCCAGTTGGT CCACCTGGAG TTCCAGGCCG ACCTGGACAA 
GATGGCCTAC CAGGACTCCC TGGATCTAAG GGAGAAATGG GCTCAATGGG GCCTCCCGGA 
TCAAAAGGGA ACCCCGGCAA CCCTGGAGCT CCCGGCTTTC CAGGACCAAA AGGAGATGAT 
GGTGTCCCAG GACAGTCAGG AAATCCAGGT TTTCCAGGGT TAAAAGGAGA CAAAGGTGAA 
ACAGGCATTC AAGGCCCGCC TGGCATAACC ACATCTACAA GTGTACTGAA AGGACAAAAA 
GGAGAATCTG GTGTTCCAGG TTTCAGTGGA CCGCCAGGAC CAAAGGGTAT TACAGGTATC 
TCTGGAGATC CTGGTCTGCC TGGGCAAGAT GGACGACCAG GACTACCAGG ACCCCCAGGC 
CCCAAAGGTG ATGCTGGTCT CCCAGGAATT CCAGGTTCTC CAGGAGCCAC AGGATTAAAG 
GGGGCCATGG GAGAGATGGG ATTGCCAGGA GTTCCTGGAG TCAAAGGAAA TTCCGGCCAA 
CCTGGCCAAC CTGGCCAGCC AGGATCCCCA GGTGGACCAG GATTCCCAGG ACCTAAAGGT 
GAAGCTGGTG TACCAGGATA CGGATTACCT GGAAATCCCG GACCTAAGGG TGAACCAGGC 
ACTCCAGGCT TTCCTGGACC CGCTGGCTTG AAAGGCCAGC CTGGCTTTCC AGGACCTCAA 
GGCTTGACTG GTAGCCCTGG ACCAAAGGGT GATCCGGGAC TCCCAGGATT TCAAGGTGCA 
CCTGGCAATC CTGGCCCAAA AGGTTTGGAT GGTGCTCCAG GCCCTGCAGG TTTAAATGGA 
GCACCTGGTA GGCCAGGAGA TCCCGGACGA TCTGGATTAC CTGGTATCCC TGGAGATAAA 
GGTCTTGCTG GTCGTGATGG TATTCCAGGA CCAGCAGGTC AAAAGGGTGA ACCAGGTGCT 
CCAGGCCTTG GTGGCCCAGG CTTGCCTGGT CAACCTGGTT TGAAAGGCGC TAAAGGAGAT 
CCTGGTTTTC CGGGTCCTGC CGGTAGTGCT GGATTTCCTG GTCCAAAGGG TGATGCAGGA 
TTTCCTGGCT TGCCTGGTCA GATTGGTTCT GTAGGACCAC CTGGGCCTCC AGGCTTGCCA 
CTGCAGGGAC CTAAAGGTAT CCAAGGTCCA CCTGGTCCCC CCGGAAGAGC TGGTCCTCCT 
GGCCTTGAGG GTCCACGTGG ACCTCCAGGT AGCGGAGGAG TGAAGGGTGA GAAGGGTTTG 
CCTGGAGTTC CTGGGACGCC TGGAGAAGCA GGACAGAAAG GAGATTATGG TTCTCCTGGG 
TTTCCGGGCT CCCCAGGGAG TCCAGGACCA AATGGCCTCA AAGGAGATCC TGGACCTCAA 
GGTGTTCCTG GTTTTCCAGG ATCTAAAGGA GTGTCTGGTC CACCTGGTAT TTCTGGCCTC 
CCTGGAGCAC GTGGTGATCC TGGAGATAAA GGACCTCCCG GACCCCCAGG TTATGCTGCA 
GAGCCTATTG TCTTGAAGGG CGAGCGTGGC CCACCAGGAC CTCCAGGTAA TCCAGGCGGC 
AGAGGACAAG CCGGACCCAT CGGATTGCAG GGACTCCCAG GTTCGCCTGG CAGCTCTGGA 
GACCCAGGAC CAATGGGACC TCCAGGATTT AATGGCCCAC CTGGAAGGAA GGGAGACACA 
GGTCCAGCTG GACAGCCAGG TCAGCGTGGT TACCCCGGCC CTCCAGGTCC AGATGGGGCT 
CCGGGGCCTC CAGGTATCAC GGGAACTTTG TCTGGTGCAC ACGGTTTTCT GATCACACGC 
CACAGTCAGA CCATAGAAGT GCCATCGTGT CCTGATGGCA CAAGCCCAAT CTACGAGGGC 
TACTCTTTAC TCTACGTGCA AGGCAACGAG CGGGCACACG GTCAAGATCT GGGTACGGCT 
GGCAGCTGTC TACGGCGTTT CAGCACGATG CCTTTCATGT TCTGCAACAT CAACAATGTC 
TGTAACTTCG CTTCCCGTAA TGATTACTCA TACTGGCTGT CCACACCACA GCCCATGCCC 
ATGACCATGG CACCCATCAC TGGAGAGAAC ATCAAACCTT ACATCAGCCG GTGTTCTGTG 
TGTGAAGCTC CAGCAATGGT GATTGCTGTA CACAGTCAGA CCATTCAGTT GCCAGCGTGC 
CCTGAAAACT GGGATACCCT CTGGATCGGT TACTCCTTCA TGATGCACAC CAGTGCAGGT 
GCTGAGGGTT CAGGTCAGGC TCTGGCCTCT CCGGGCTCCT GTCTGGAGGA GTTTCGCTCC 
GCTCCCTTCA TTGAGTGCCA CGGACGAGGA ACCTGCAATT ATTACGGCAA CTCCTACAGC 
TTCTGGCTGG CCACTGTGGA CGTGAACGAA ATGTTCAGAA AGCCGCAGTC AGAGACTCTA 
AAAGCTGGAA ACTTGAGGAC CCGCGTGAGC CGCTGTGTGG TGTGTATGAA GAGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166559.1
CDS	185..5224
Translation	MNLKSLGAVLLLVLTLSAGVEKSESAACHGCGTGSKCDCSSVKGVKGDRGLPGLKGQPGVPGFPGPEGPMGPRGEKGDDGPQGSIGPKGIRGPPGLPGFSGTPGIPGLPGQDGAPGPRGIPGCNGTKGERGFPGNPGRSGLDGQPGPPGLIGQKGEPGEVIDINRISENGLPGFNGFTGRRGDPGPPGLQGPIGPPGPKGFEGRPGPPGPPGPKGNMGLNFQGPKGEKGDRGLKGPPGPPGQVGEVSGSPDTVIQQGEKGDRGIPGDTGFPGPPGPPGPQGGQKGEKGEPGEAGKRGKPGKDGEPGLLGYPGDKGDQGPPGLPGRDGFRGEKGDQGPPGPPSYRGDQFTGVGGIGPKGDRGDPGPSGIKGDRGLPGIQGPPGPQGPPGIGGPGPPGSQGLPGERGEKGDEGQPGISLPGPPGRPGPTGPQGIQGPPGPPGPFNSDVECNRGLPGPPGLQGERGFTGDSGQKGQKGETCVNCFSNGNPVPGPQGFPGPPGSPGAPGASGSKGDRGYPGGPGLMGPPGIPGPQGSPGLPGEKGDPGDTLSVLGVKGDKGDTGFPGSPGLPGLEGRPGRDGVPGPPGLRGPPGLGLKGTQGPNGEPGLPGPPGDRGLPGSPGFGPQGPSGEKGIQGVSGRPGAPGLPGQKGEPGMTVPEVGERGPPGQDGDPGSQGRPGDSGPPGQPGFPGLPGSKGEPGLPGIGLPGPPGAKGFPGIAGSPGGPGIPGRPGLDGLPGQPGLPGSKGDPGYGLPGPPGPTGSPGIKGGPGPKGDSGFPGSPGQPGRPGLDGAPGPKGDAGFPGGPGPRGPPGAPAFGLQGPPGPPGAPGSIGSPGVPGANGEKGDRGPPGLSTPGFQGDRGISGLPGPPGPVGPPGVPGRPGQDGLPGLPGSKGEMGSMGPPGSKGNPGNPGAPGFPGPKGDDGVPGQSGNPGFPGLKGDKGETGIQGPPGITTSTSVLKGQKGESGVPGFSGPPGPKGITGISGDPGLPGQDGRPGLPGPPGPKGDAGLPGIPGSPGATGLKGAMGEMGLPGVPGVKGNSGQPGQPGQPGSPGGPGFPGPKGEAGVPGYGLPGNPGPKGEPGTPGFPGPAGLKGQPGFPGPQGLTGSPGPKGDPGLPGFQGAPGNPGPKGLDGAPGPAGLNGAPGRPGDPGRSGLPGIPGDKGLAGRDGIPGPAGQKGEPGAPGLGGPGLPGQPGLKGAKGDPGFPGPAGSAGFPGPKGDAGFPGLPGQIGSVGPPGPPGLPLQGPKGIQGPPGPPGRAGPPGLEGPRGPPGSGGVKGEKGLPGVPGTPGEAGQKGDYGSPGFPGSPGSPGPNGLKGDPGPQGVPGFPGSKGVSGPPGISGLPGARGDPGDKGPPGPPGYAAEPIVLKGERGPPGPPGNPGGRGQAGPIGLQGLPGSPGSSGDPGPMGPPGFNGPPGRKGDTGPAGQPGQRGYPGPPGPDGAPGPPGITGTLSGAHGFLITRHSQTIEVPSCPDGTSPIYEGYSLLYVQGNERAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPQPMPMTMAPITGENIKPYISRCSVCEAPAMVIAVHSQTIQLPACPENWDTLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYGNSYSFWLATVDVNEMFRKPQSETLKAGNLRTRVSRCVVCMKRT

Target ORF information:

RefSeq Version	XM_005166502.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166502.2

ORF Insert Sequence:

ATGAACTTAA AAAGTCTCGG CGCGGTGTTG CTGCTTGTGC TCACTCTGAG TGCGGGAGTA 
GAGAAATCCG AGAGTGCGGC ATGTCATGGT TGTGGGACTG GATCAAAATG TGACTGCAGT 
TCAGTGAAAG GAGTGAAGGG AGATCGAGGA CTTCCGGGTT TAAAGGGTCA GCCGGGTGTT 
CCAGGTTTCC CAGGACCAGA GGGACCAATG GGACCTCGTG GAGAGAAAGG TGATGACGGA 
CCTCAAGGGT CGATAGGCCC CAAAGGAATA AGAGGACCTC CAGGATTACC TGGATTTTCA 
GGAACACCAG GAATCCCAGG TCTTCCAGGA CAGGATGGTG CTCCAGGACC CAGAGGAATT 
CCAGGCTGTA ATGGAACTAA AGGTGAAAGA GGATTCCCTG GAAATCCAGG TCGCTCAGGT 
TTAGATGGTC AACCAGGTCC ACCAGGTCTT ATAGGACAAA AAGGAGAACC TGGGGAAGTC 
ATCGACATTA ACCGCATTTC AGAAAATGGA CTTCCAGGCT TTAATGGCTT TACAGGCAGA 
AGGGGTGATC CCGGACCCCC AGGTCTTCAA GGGCCTATCG GTCCTCCTGG TCCAAAAGGG 
TTTGAGGGTC GCCCAGGCCC TCCTGGTCCA CCGGGACCAA AAGGAAACAT GGGATTGAAT 
TTCCAAGGAC CCAAAGGAGA GAAGGGTGAC CGTGGATTAA AAGGACCTCC AGGGCCACCA 
GGACAAGTTG GTGAAGTGTC AGGTTCACCA GATACAGTCA TTCAACAAGG AGAAAAGGGT 
GATCGTGGCA TACCTGGAGA TACTGGTTTC CCAGGGCCCC CTGGACCCCC TGGTCCACAA 
GGAGGTCAGA AAGGAGAGAA AGGAGAACCT GGGGAGGCTG GAAAGAGGGG AAAACCTGGC 
AAAGATGGTG AACCAGGTCT TCTTGGATAT CCGGGTGATA AAGGAGACCA AGGGCCTCCT 
GGTCTGCCTG GACGAGATGG TTTTAGAGGT GAGAAAGGTG ATCAAGGGCC ACCTGGACCT 
CCCTCCTACC GGGGTGATCA GTTCACAGGT GTAGGTGGAA TAGGACCAAA AGGAGACCGT 
GGTGATCCAG GACCCTCTGG TATTAAAGGA GACAGAGGAC TACCAGGTAT TCAGGGGCCA 
CCTGGTCCAC AGGGTCCTCC AGGAATTGGA GGTCCAGGAC CTCCCGGTTC ACAAGGATTG 
CCTGGAGAAC GAGGAGAAAA GGGTGATGAG GGACAGCCTG GAATCTCACT TCCTGGACCA 
CCCGGGAGAC CCGGACCAAC AGGACCACAA GGAATACAAG GACCACCTGG CCCCCCAGGA 
CCATTCAACA GTGATGTAGA ATGTAATCGT GGTCTTCCTG GTCCTCCAGG CCTGCAGGGG 
GAGCGTGGGT TTACTGGAGA TAGTGGGCAG AAAGGTCAAA AAGGCGAGAC TTGTGTGAAT 
TGTTTCTCCA ATGGAAACCC TGTCCCAGGA CCCCAAGGTT TTCCAGGTCC TCCTGGAAGT 
CCAGGTGCTC CTGGGGCGTC TGGATCTAAA GGAGACAGGG GATATCCGGG AGGACCAGGG 
CTTATGGGCC CACCTGGAAT ACCTGGACCT CAGGGTTCCC CTGGACTCCC TGGAGAGAAG 
GGTGACCCAG GCGATACCCT CTCTGTTCTT GGCGTGAAGG GAGATAAAGG AGACACAGGT 
TTTCCTGGCT CTCCAGGTCT GCCGGGACTC GAAGGAAGAC CTGGACGTGA TGGAGTGCCT 
GGTCCACCTG GCCTCAGAGG ACCACCTGGT TTGGGACTGA AAGGTACACA AGGTCCCAAC 
GGTGAACCTG GTTTGCCAGG ACCTCCTGGA GACAGAGGTT TACCAGGTTC CCCAGGATTT 
GGTCCTCAAG GACCTTCTGG AGAAAAAGGT ATTCAGGGTG TTTCAGGAAG ACCAGGGGCA 
CCTGGACTAC CAGGTCAGAA AGGTGAACCT GGGATGACTG TGCCAGAAGT TGGTGAACGT 
GGACCCCCAG GACAGGATGG AGATCCTGGT TCACAAGGAC GTCCAGGTGA TTCAGGCCCA 
CCTGGACAGC CTGGGTTCCC AGGTTTGCCT GGATCCAAAG GAGAGCCTGG TCTTCCTGGT 
ATTGGTTTGC CAGGACCTCC TGGTGCTAAA GGATTTCCTG GTATTGCTGG TTCCCCTGGT 
GGACCTGGCA TTCCTGGACG ACCAGGTTTA GATGGACTGC CTGGTCAGCC TGGATTACCT 
GGATCCAAAG GAGATCCTGG TTATGGTCTG CCTGGTCCTC CAGGTCCCAC AGGATCACCA 
GGCATTAAAG GTGGACCCGG TCCGAAAGGT GATTCTGGTT TCCCTGGAAG CCCTGGTCAA 
CCAGGCCGCC CAGGTCTCGA TGGGGCTCCT GGACCTAAAG GTGATGCTGG TTTTCCTGGT 
GGTCCTGGTC CTCGTGGCCC CCCTGGGGCT CCTGCTTTTG GTCTTCAGGG CCCTCCTGGT 
CCTCCTGGAG CTCCAGGCTC TATTGGATCT CCTGGAGTAC CGGGGGCGAA TGGAGAAAAA 
GGAGACAGAG GCCCTCCAGG TCTGAGTACT CCAGGCTTCC AGGGTGATAG AGGAATTTCT 
GGACTTCCTG GACCTCCTGG TCCAGTTGGT CCACCTGGAG TTCCAGGCCG ACCTGGACAA 
GATGGCCTAC CAGGACTCCC TGGATCTAAG GGAGAAATGG GCTCAATGGG GCCTCCCGGA 
TCAAAAGGGA ACCCCGGCAA CCCTGGAGCT CCCGGCTTTC CAGGACCAAA AGGAGATGAT 
GGTGTCCCAG GACAGTCAGG AAATCCAGGT TTTCCAGGGT TAAAAGGAGA CAAAGGTGAA 
ACAGGCATTC AAGGCCCGCC TGGCATAACC ACATCTACAA GTGTACTGAA AGGACAAAAA 
GGAGAATCTG GTGTTCCAGG TTTCAGTGGA CCGCCAGGAC CAAAGGGTAT TACAGGTATC 
TCTGGAGATC CTGGTCTGCC TGGGCAAGAT GGACGACCAG GACTACCAGG ACCCCCAGGC 
CCCAAAGGTG ATGCTGGTCT CCCAGGAATT CCAGGTTCTC CAGGAGCCAC AGGATTAAAG 
GGGGCCATGG GAGAGATGGG ATTGCCAGGA GTTCCTGGAG TCAAAGGAAA TTCCGGCCAA 
CCTGGCCAAC CTGGCCAGCC AGGATCCCCA GGTGGACCAG GATTCCCAGG ACCTAAAGGT 
GAAGCTGGTG TACCAGGATA CGGATTACCT GGAAATCCCG GACCTAAGGG TGAACCAGGC 
ACTCCAGGCT TTCCTGGACC CGCTGGCTTG AAAGGCCAGC CTGGCTTTCC AGGACCTCAA 
GGCTTGACTG GTAGCCCTGG ACCAAAGGGT GATCCGGGAC TCCCAGGATT TCAAGGTGCA 
CCTGGCAATC CTGGCCCAAA AGGTTTGGAT GGTGCTCCAG GCCCTGCAGG TTTAAATGGA 
GCACCTGGTA GGCCAGGAGA TCCCGGACGA TCTGGATTAC CTGGTATCCC TGGAGATAAA 
GGTCTTGCTG GTCGTGATGG TATTCCAGGA CCAGCAGGTC AAAAGGGTGA ACCAGGTGCT 
CCAGGCCTTG GTGGCCCAGG CTTGCCTGGT CAACCTGGTT TGAAAGGCGC TAAAGGAGAT 
CCTGGTTTTC CGGGTCCTGC CGGTAGTGCT GGATTTCCTG GTCCAAAGGG TGATGCAGGA 
TTTCCTGGCT TGCCTGGTCA GATTGGTTCT GTAGGACCAC CTGGGCCTCC AGGCTTGCCA 
CTGCAGGGAC CTAAAGGTAT CCAAGGTCCA CCTGGTCCCC CCGGAAGAGC TGGTCCTCCT 
GGCCTTGAGG GTCCACGTGG ACCTCCAGGT AGCGGAGGAG TGAAGGGTGA GAAGGGTTTG 
CCTGGAGTTC CTGGGACGCC TGGAGAAGCA GGACAGAAAG GAGATTATGG TTCTCCTGGG 
TTTCCGGGCT CCCCAGGGAG TCCAGGACCA AATGGCCTCA AAGGAGATCC TGGACCTCAA 
GGTGTTCCTG GTTTTCCAGG ATCTAAAGGA GTGTCTGGTC CACCTGGTAT TTCTGGCCTC 
CCTGGAGCAC GTGGTGATCC TGGAGATAAA GGACCTCCCG GACCCCCAGG TTATGCTGCA 
GAGCCTATTG TCTTGAAGGG CGAGCGTGGC CCACCAGGAC CTCCAGGTAA TCCAGGCGGC 
AGAGGACAAG CCGGACCCAT CGGATTGCAG GGACTCCCAG GTTCGCCTGG CAGCTCTGGA 
GACCCAGGAC CAATGGGACC TCCAGGATTT AATGGCCCAC CTGGAAGGAA GGGAGACACA 
GGTCCAGCTG GACAGCCAGG TCAGCGTGGT TACCCCGGCC CTCCAGGTCC AGATGGGGCT 
CCGGGGCCTC CAGGTATCAC GGGAACTTTG TCTGGTGCAC ACGGTTTTCT GATCACACGC 
CACAGTCAGA CCATAGAAGT GCCATCGTGT CCTGATGGCA CAAGCCCAAT CTACGAGGGC 
TACTCTTTAC TCTACGTGCA AGGCAACGAG CGGGCACACG GTCAAGATCT GGGTACGGCT 
GGCAGCTGTC TACGGCGTTT CAGCACGATG CCTTTCATGT TCTGCAACAT CAACAATGTC 
TGTAACTTCG CTTCCCGTAA TGATTACTCA TACTGGCTGT CCACACCACA GCCCATGCCC 
ATGACCATGG CACCCATCAC TGGAGAGAAC ATCAAACCTT ACATCAGCCG GTGTTCTGTG 
TGTGAAGCTC CAGCAATGGT GATTGCTGTA CACAGTCAGA CCATTCAGTT GCCAGCGTGC 
CCTGAAAACT GGGATACCCT CTGGATCGGT TACTCCTTCA TGATGCACAC CAGTGCAGGT 
GCTGAGGGTT CAGGTCAGGC TCTGGCCTCT CCGGGCTCCT GTCTGGAGGA GTTTCGCTCC 
GCTCCCTTCA TTGAGTGCCA CGGACGAGGA ACCTGCAATT ATTACGGCAA CTCCTACAGC 
TTCTGGCTGG CCACTGTGGA CGTGAACGAA ATGTTCAGAA AGCCGCAGTC AGAGACTCTA 
AAAGCTGGAA ACTTGAGGAC CCGCGTGAGC CGCTGTGTGG TGTGTATGAA GAGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a5 ( XM_005166502.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166559.1 Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13419
Clone ID Related Accession (Same CDS sequence)	XM_005166502.2 , XM_005166502.3
Accession Version	XM_005166502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTAA AAAGTCTCGG CGCGGTGTTG CTGCTTGTGC TCACTCTGAG TGCGGGAGTA 
GAGAAATCCG AGAGTGCGGC ATGTCATGGT TGTGGGACTG GATCAAAATG TGACTGCAGT 
TCAGTGAAAG GAGTGAAGGG AGATCGAGGA CTTCCGGGTT TAAAGGGTCA GCCGGGTGTT 
CCAGGTTTCC CAGGACCAGA GGGACCAATG GGACCTCGTG GAGAGAAAGG TGATGACGGA 
CCTCAAGGGT CGATAGGCCC CAAAGGAATA AGAGGACCTC CAGGATTACC TGGATTTTCA 
GGAACACCAG GAATCCCAGG TCTTCCAGGA CAGGATGGTG CTCCAGGACC CAGAGGAATT 
CCAGGCTGTA ATGGAACTAA AGGTGAAAGA GGATTCCCTG GAAATCCAGG TCGCTCAGGT 
TTAGATGGTC AACCAGGTCC ACCAGGTCTT ATAGGACAAA AAGGAGAACC TGGGGAAGTC 
ATCGACATTA ACCGCATTTC AGAAAATGGA CTTCCAGGCT TTAATGGCTT TACAGGCAGA 
AGGGGTGATC CCGGACCCCC AGGTCTTCAA GGGCCTATCG GTCCTCCTGG TCCAAAAGGG 
TTTGAGGGTC GCCCAGGCCC TCCTGGTCCA CCGGGACCAA AAGGAAACAT GGGATTGAAT 
TTCCAAGGAC CCAAAGGAGA GAAGGGTGAC CGTGGATTAA AAGGACCTCC AGGGCCACCA 
GGACAAGTTG GTGAAGTGTC AGGTTCACCA GATACAGTCA TTCAACAAGG AGAAAAGGGT 
GATCGTGGCA TACCTGGAGA TACTGGTTTC CCAGGGCCCC CTGGACCCCC TGGTCCACAA 
GGAGGTCAGA AAGGAGAGAA AGGAGAACCT GGGGAGGCTG GAAAGAGGGG AAAACCTGGC 
AAAGATGGTG AACCAGGTCT TCTTGGATAT CCGGGTGATA AAGGAGACCA AGGGCCTCCT 
GGTCTGCCTG GACGAGATGG TTTTAGAGGT GAGAAAGGTG ATCAAGGGCC ACCTGGACCT 
CCCTCCTACC GGGGTGATCA GTTCACAGGT GTAGGTGGAA TAGGACCAAA AGGAGACCGT 
GGTGATCCAG GACCCTCTGG TATTAAAGGA GACAGAGGAC TACCAGGTAT TCAGGGGCCA 
CCTGGTCCAC AGGGTCCTCC AGGAATTGGA GGTCCAGGAC CTCCCGGTTC ACAAGGATTG 
CCTGGAGAAC GAGGAGAAAA GGGTGATGAG GGACAGCCTG GAATCTCACT TCCTGGACCA 
CCCGGGAGAC CCGGACCAAC AGGACCACAA GGAATACAAG GACCACCTGG CCCCCCAGGA 
CCATTCAACA GTGATGTAGA ATGTAATCGT GGTCTTCCTG GTCCTCCAGG CCTGCAGGGG 
GAGCGTGGGT TTACTGGAGA TAGTGGGCAG AAAGGTCAAA AAGGCGAGAC TTGTGTGAAT 
TGTTTCTCCA ATGGAAACCC TGTCCCAGGA CCCCAAGGTT TTCCAGGTCC TCCTGGAAGT 
CCAGGTGCTC CTGGGGCGTC TGGATCTAAA GGAGACAGGG GATATCCGGG AGGACCAGGG 
CTTATGGGCC CACCTGGAAT ACCTGGACCT CAGGGTTCCC CTGGACTCCC TGGAGAGAAG 
GGTGACCCAG GCGATACCCT CTCTGTTCTT GGCGTGAAGG GAGATAAAGG AGACACAGGT 
TTTCCTGGCT CTCCAGGTCT GCCGGGACTC GAAGGAAGAC CTGGACGTGA TGGAGTGCCT 
GGTCCACCTG GCCTCAGAGG ACCACCTGGT TTGGGACTGA AAGGTACACA AGGTCCCAAC 
GGTGAACCTG GTTTGCCAGG ACCTCCTGGA GACAGAGGTT TACCAGGTTC CCCAGGATTT 
GGTCCTCAAG GACCTTCTGG AGAAAAAGGT ATTCAGGGTG TTTCAGGAAG ACCAGGGGCA 
CCTGGACTAC CAGGTCAGAA AGGTGAACCT GGGATGACTG TGCCAGAAGT TGGTGAACGT 
GGACCCCCAG GACAGGATGG AGATCCTGGT TCACAAGGAC GTCCAGGTGA TTCAGGCCCA 
CCTGGACAGC CTGGGTTCCC AGGTTTGCCT GGATCCAAAG GAGAGCCTGG TCTTCCTGGT 
ATTGGTTTGC CAGGACCTCC TGGTGCTAAA GGATTTCCTG GTATTGCTGG TTCCCCTGGT 
GGACCTGGCA TTCCTGGACG ACCAGGTTTA GATGGACTGC CTGGTCAGCC TGGATTACCT 
GGATCCAAAG GAGATCCTGG TTATGGTCTG CCTGGTCCTC CAGGTCCCAC AGGATCACCA 
GGCATTAAAG GTGGACCCGG TCCGAAAGGT GATTCTGGTT TCCCTGGAAG CCCTGGTCAA 
CCAGGCCGCC CAGGTCTCGA TGGGGCTCCT GGACCTAAAG GTGATGCTGG TTTTCCTGGT 
GGTCCTGGTC CTCGTGGCCC CCCTGGGGCT CCTGCTTTTG GTCTTCAGGG CCCTCCTGGT 
CCTCCTGGAG CTCCAGGCTC TATTGGATCT CCTGGAGTAC CGGGGGCGAA TGGAGAAAAA 
GGAGACAGAG GCCCTCCAGG TCTGAGTACT CCAGGCTTCC AGGGTGATAG AGGAATTTCT 
GGACTTCCTG GACCTCCTGG TCCAGTTGGT CCACCTGGAG TTCCAGGCCG ACCTGGACAA 
GATGGCCTAC CAGGACTCCC TGGATCTAAG GGAGAAATGG GCTCAATGGG GCCTCCCGGA 
TCAAAAGGGA ACCCCGGCAA CCCTGGAGCT CCCGGCTTTC CAGGACCAAA AGGAGATGAT 
GGTGTCCCAG GACAGTCAGG AAATCCAGGT TTTCCAGGGT TAAAAGGAGA CAAAGGTGAA 
ACAGGCATTC AAGGCCCGCC TGGCATAACC ACATCTACAA GTGTACTGAA AGGACAAAAA 
GGAGAATCTG GTGTTCCAGG TTTCAGTGGA CCGCCAGGAC CAAAGGGTAT TACAGGTATC 
TCTGGAGATC CTGGTCTGCC TGGGCAAGAT GGACGACCAG GACTACCAGG ACCCCCAGGC 
CCCAAAGGTG ATGCTGGTCT CCCAGGAATT CCAGGTTCTC CAGGAGCCAC AGGATTAAAG 
GGGGCCATGG GAGAGATGGG ATTGCCAGGA GTTCCTGGAG TCAAAGGAAA TTCCGGCCAA 
CCTGGCCAAC CTGGCCAGCC AGGATCCCCA GGTGGACCAG GATTCCCAGG ACCTAAAGGT 
GAAGCTGGTG TACCAGGATA CGGATTACCT GGAAATCCCG GACCTAAGGG TGAACCAGGC 
ACTCCAGGCT TTCCTGGACC CGCTGGCTTG AAAGGCCAGC CTGGCTTTCC AGGACCTCAA 
GGCTTGACTG GTAGCCCTGG ACCAAAGGGT GATCCGGGAC TCCCAGGATT TCAAGGTGCA 
CCTGGCAATC CTGGCCCAAA AGGTTTGGAT GGTGCTCCAG GCCCTGCAGG TTTAAATGGA 
GCACCTGGTA GGCCAGGAGA TCCCGGACGA TCTGGATTAC CTGGTATCCC TGGAGATAAA 
GGTCTTGCTG GTCGTGATGG TATTCCAGGA CCAGCAGGTC AAAAGGGTGA ACCAGGTGCT 
CCAGGCCTTG GTGGCCCAGG CTTGCCTGGT CAACCTGGTT TGAAAGGCGC TAAAGGAGAT 
CCTGGTTTTC CGGGTCCTGC CGGTAGTGCT GGATTTCCTG GTCCAAAGGG TGATGCAGGA 
TTTCCTGGCT TGCCTGGTCA GATTGGTTCT GTAGGACCAC CTGGGCCTCC AGGCTTGCCA 
CTGCAGGGAC CTAAAGGTAT CCAAGGTCCA CCTGGTCCCC CCGGAAGAGC TGGTCCTCCT 
GGCCTTGAGG GTCCACGTGG ACCTCCAGGT AGCGGAGGAG TGAAGGGTGA GAAGGGTTTG 
CCTGGAGTTC CTGGGACGCC TGGAGAAGCA GGACAGAAAG GAGATTATGG TTCTCCTGGG 
TTTCCGGGCT CCCCAGGGAG TCCAGGACCA AATGGCCTCA AAGGAGATCC TGGACCTCAA 
GGTGTTCCTG GTTTTCCAGG ATCTAAAGGA GTGTCTGGTC CACCTGGTAT TTCTGGCCTC 
CCTGGAGCAC GTGGTGATCC TGGAGATAAA GGACCTCCCG GACCCCCAGG TTATGCTGCA 
GAGCCTATTG TCTTGAAGGG CGAGCGTGGC CCACCAGGAC CTCCAGGTAA TCCAGGCGGC 
AGAGGACAAG CCGGACCCAT CGGATTGCAG GGACTCCCAG GTTCGCCTGG CAGCTCTGGA 
GACCCAGGAC CAATGGGACC TCCAGGATTT AATGGCCCAC CTGGAAGGAA GGGAGACACA 
GGTCCAGCTG GACAGCCAGG TCAGCGTGGT TACCCCGGCC CTCCAGGTCC AGATGGGGCT 
CCGGGGCCTC CAGGTATCAC GGGAACTTTG TCTGGTGCAC ACGGTTTTCT GATCACACGC 
CACAGTCAGA CCATAGAAGT GCCATCGTGT CCTGATGGCA CAAGCCCAAT CTACGAGGGC 
TACTCTTTAC TCTACGTGCA AGGCAACGAG CGGGCACACG GTCAAGATCT GGGTACGGCT 
GGCAGCTGTC TACGGCGTTT CAGCACGATG CCTTTCATGT TCTGCAACAT CAACAATGTC 
TGTAACTTCG CTTCCCGTAA TGATTACTCA TACTGGCTGT CCACACCACA GCCCATGCCC 
ATGACCATGG CACCCATCAC TGGAGAGAAC ATCAAACCTT ACATCAGCCG GTGTTCTGTG 
TGTGAAGCTC CAGCAATGGT GATTGCTGTA CACAGTCAGA CCATTCAGTT GCCAGCGTGC 
CCTGAAAACT GGGATACCCT CTGGATCGGT TACTCCTTCA TGATGCACAC CAGTGCAGGT 
GCTGAGGGTT CAGGTCAGGC TCTGGCCTCT CCGGGCTCCT GTCTGGAGGA GTTTCGCTCC 
GCTCCCTTCA TTGAGTGCCA CGGACGAGGA ACCTGCAATT ATTACGGCAA CTCCTACAGC 
TTCTGGCTGG CCACTGTGGA CGTGAACGAA ATGTTCAGAA AGCCGCAGTC AGAGACTCTA 
AAAGCTGGAA ACTTGAGGAC CCGCGTGAGC CGCTGTGTGG TGTGTATGAA GAGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166559.1
CDS	189..5228
Translation	MNLKSLGAVLLLVLTLSAGVEKSESAACHGCGTGSKCDCSSVKGVKGDRGLPGLKGQPGVPGFPGPEGPMGPRGEKGDDGPQGSIGPKGIRGPPGLPGFSGTPGIPGLPGQDGAPGPRGIPGCNGTKGERGFPGNPGRSGLDGQPGPPGLIGQKGEPGEVIDINRISENGLPGFNGFTGRRGDPGPPGLQGPIGPPGPKGFEGRPGPPGPPGPKGNMGLNFQGPKGEKGDRGLKGPPGPPGQVGEVSGSPDTVIQQGEKGDRGIPGDTGFPGPPGPPGPQGGQKGEKGEPGEAGKRGKPGKDGEPGLLGYPGDKGDQGPPGLPGRDGFRGEKGDQGPPGPPSYRGDQFTGVGGIGPKGDRGDPGPSGIKGDRGLPGIQGPPGPQGPPGIGGPGPPGSQGLPGERGEKGDEGQPGISLPGPPGRPGPTGPQGIQGPPGPPGPFNSDVECNRGLPGPPGLQGERGFTGDSGQKGQKGETCVNCFSNGNPVPGPQGFPGPPGSPGAPGASGSKGDRGYPGGPGLMGPPGIPGPQGSPGLPGEKGDPGDTLSVLGVKGDKGDTGFPGSPGLPGLEGRPGRDGVPGPPGLRGPPGLGLKGTQGPNGEPGLPGPPGDRGLPGSPGFGPQGPSGEKGIQGVSGRPGAPGLPGQKGEPGMTVPEVGERGPPGQDGDPGSQGRPGDSGPPGQPGFPGLPGSKGEPGLPGIGLPGPPGAKGFPGIAGSPGGPGIPGRPGLDGLPGQPGLPGSKGDPGYGLPGPPGPTGSPGIKGGPGPKGDSGFPGSPGQPGRPGLDGAPGPKGDAGFPGGPGPRGPPGAPAFGLQGPPGPPGAPGSIGSPGVPGANGEKGDRGPPGLSTPGFQGDRGISGLPGPPGPVGPPGVPGRPGQDGLPGLPGSKGEMGSMGPPGSKGNPGNPGAPGFPGPKGDDGVPGQSGNPGFPGLKGDKGETGIQGPPGITTSTSVLKGQKGESGVPGFSGPPGPKGITGISGDPGLPGQDGRPGLPGPPGPKGDAGLPGIPGSPGATGLKGAMGEMGLPGVPGVKGNSGQPGQPGQPGSPGGPGFPGPKGEAGVPGYGLPGNPGPKGEPGTPGFPGPAGLKGQPGFPGPQGLTGSPGPKGDPGLPGFQGAPGNPGPKGLDGAPGPAGLNGAPGRPGDPGRSGLPGIPGDKGLAGRDGIPGPAGQKGEPGAPGLGGPGLPGQPGLKGAKGDPGFPGPAGSAGFPGPKGDAGFPGLPGQIGSVGPPGPPGLPLQGPKGIQGPPGPPGRAGPPGLEGPRGPPGSGGVKGEKGLPGVPGTPGEAGQKGDYGSPGFPGSPGSPGPNGLKGDPGPQGVPGFPGSKGVSGPPGISGLPGARGDPGDKGPPGPPGYAAEPIVLKGERGPPGPPGNPGGRGQAGPIGLQGLPGSPGSSGDPGPMGPPGFNGPPGRKGDTGPAGQPGQRGYPGPPGPDGAPGPPGITGTLSGAHGFLITRHSQTIEVPSCPDGTSPIYEGYSLLYVQGNERAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPQPMPMTMAPITGENIKPYISRCSVCEAPAMVIAVHSQTIQLPACPENWDTLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYGNSYSFWLATVDVNEMFRKPQSETLKAGNLRTRVSRCVVCMKRT

Target ORF information:

RefSeq Version	XM_005166502.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166502.3

ORF Insert Sequence:

ATGAACTTAA AAAGTCTCGG CGCGGTGTTG CTGCTTGTGC TCACTCTGAG TGCGGGAGTA 
GAGAAATCCG AGAGTGCGGC ATGTCATGGT TGTGGGACTG GATCAAAATG TGACTGCAGT 
TCAGTGAAAG GAGTGAAGGG AGATCGAGGA CTTCCGGGTT TAAAGGGTCA GCCGGGTGTT 
CCAGGTTTCC CAGGACCAGA GGGACCAATG GGACCTCGTG GAGAGAAAGG TGATGACGGA 
CCTCAAGGGT CGATAGGCCC CAAAGGAATA AGAGGACCTC CAGGATTACC TGGATTTTCA 
GGAACACCAG GAATCCCAGG TCTTCCAGGA CAGGATGGTG CTCCAGGACC CAGAGGAATT 
CCAGGCTGTA ATGGAACTAA AGGTGAAAGA GGATTCCCTG GAAATCCAGG TCGCTCAGGT 
TTAGATGGTC AACCAGGTCC ACCAGGTCTT ATAGGACAAA AAGGAGAACC TGGGGAAGTC 
ATCGACATTA ACCGCATTTC AGAAAATGGA CTTCCAGGCT TTAATGGCTT TACAGGCAGA 
AGGGGTGATC CCGGACCCCC AGGTCTTCAA GGGCCTATCG GTCCTCCTGG TCCAAAAGGG 
TTTGAGGGTC GCCCAGGCCC TCCTGGTCCA CCGGGACCAA AAGGAAACAT GGGATTGAAT 
TTCCAAGGAC CCAAAGGAGA GAAGGGTGAC CGTGGATTAA AAGGACCTCC AGGGCCACCA 
GGACAAGTTG GTGAAGTGTC AGGTTCACCA GATACAGTCA TTCAACAAGG AGAAAAGGGT 
GATCGTGGCA TACCTGGAGA TACTGGTTTC CCAGGGCCCC CTGGACCCCC TGGTCCACAA 
GGAGGTCAGA AAGGAGAGAA AGGAGAACCT GGGGAGGCTG GAAAGAGGGG AAAACCTGGC 
AAAGATGGTG AACCAGGTCT TCTTGGATAT CCGGGTGATA AAGGAGACCA AGGGCCTCCT 
GGTCTGCCTG GACGAGATGG TTTTAGAGGT GAGAAAGGTG ATCAAGGGCC ACCTGGACCT 
CCCTCCTACC GGGGTGATCA GTTCACAGGT GTAGGTGGAA TAGGACCAAA AGGAGACCGT 
GGTGATCCAG GACCCTCTGG TATTAAAGGA GACAGAGGAC TACCAGGTAT TCAGGGGCCA 
CCTGGTCCAC AGGGTCCTCC AGGAATTGGA GGTCCAGGAC CTCCCGGTTC ACAAGGATTG 
CCTGGAGAAC GAGGAGAAAA GGGTGATGAG GGACAGCCTG GAATCTCACT TCCTGGACCA 
CCCGGGAGAC CCGGACCAAC AGGACCACAA GGAATACAAG GACCACCTGG CCCCCCAGGA 
CCATTCAACA GTGATGTAGA ATGTAATCGT GGTCTTCCTG GTCCTCCAGG CCTGCAGGGG 
GAGCGTGGGT TTACTGGAGA TAGTGGGCAG AAAGGTCAAA AAGGCGAGAC TTGTGTGAAT 
TGTTTCTCCA ATGGAAACCC TGTCCCAGGA CCCCAAGGTT TTCCAGGTCC TCCTGGAAGT 
CCAGGTGCTC CTGGGGCGTC TGGATCTAAA GGAGACAGGG GATATCCGGG AGGACCAGGG 
CTTATGGGCC CACCTGGAAT ACCTGGACCT CAGGGTTCCC CTGGACTCCC TGGAGAGAAG 
GGTGACCCAG GCGATACCCT CTCTGTTCTT GGCGTGAAGG GAGATAAAGG AGACACAGGT 
TTTCCTGGCT CTCCAGGTCT GCCGGGACTC GAAGGAAGAC CTGGACGTGA TGGAGTGCCT 
GGTCCACCTG GCCTCAGAGG ACCACCTGGT TTGGGACTGA AAGGTACACA AGGTCCCAAC 
GGTGAACCTG GTTTGCCAGG ACCTCCTGGA GACAGAGGTT TACCAGGTTC CCCAGGATTT 
GGTCCTCAAG GACCTTCTGG AGAAAAAGGT ATTCAGGGTG TTTCAGGAAG ACCAGGGGCA 
CCTGGACTAC CAGGTCAGAA AGGTGAACCT GGGATGACTG TGCCAGAAGT TGGTGAACGT 
GGACCCCCAG GACAGGATGG AGATCCTGGT TCACAAGGAC GTCCAGGTGA TTCAGGCCCA 
CCTGGACAGC CTGGGTTCCC AGGTTTGCCT GGATCCAAAG GAGAGCCTGG TCTTCCTGGT 
ATTGGTTTGC CAGGACCTCC TGGTGCTAAA GGATTTCCTG GTATTGCTGG TTCCCCTGGT 
GGACCTGGCA TTCCTGGACG ACCAGGTTTA GATGGACTGC CTGGTCAGCC TGGATTACCT 
GGATCCAAAG GAGATCCTGG TTATGGTCTG CCTGGTCCTC CAGGTCCCAC AGGATCACCA 
GGCATTAAAG GTGGACCCGG TCCGAAAGGT GATTCTGGTT TCCCTGGAAG CCCTGGTCAA 
CCAGGCCGCC CAGGTCTCGA TGGGGCTCCT GGACCTAAAG GTGATGCTGG TTTTCCTGGT 
GGTCCTGGTC CTCGTGGCCC CCCTGGGGCT CCTGCTTTTG GTCTTCAGGG CCCTCCTGGT 
CCTCCTGGAG CTCCAGGCTC TATTGGATCT CCTGGAGTAC CGGGGGCGAA TGGAGAAAAA 
GGAGACAGAG GCCCTCCAGG TCTGAGTACT CCAGGCTTCC AGGGTGATAG AGGAATTTCT 
GGACTTCCTG GACCTCCTGG TCCAGTTGGT CCACCTGGAG TTCCAGGCCG ACCTGGACAA 
GATGGCCTAC CAGGACTCCC TGGATCTAAG GGAGAAATGG GCTCAATGGG GCCTCCCGGA 
TCAAAAGGGA ACCCCGGCAA CCCTGGAGCT CCCGGCTTTC CAGGACCAAA AGGAGATGAT 
GGTGTCCCAG GACAGTCAGG AAATCCAGGT TTTCCAGGGT TAAAAGGAGA CAAAGGTGAA 
ACAGGCATTC AAGGCCCGCC TGGCATAACC ACATCTACAA GTGTACTGAA AGGACAAAAA 
GGAGAATCTG GTGTTCCAGG TTTCAGTGGA CCGCCAGGAC CAAAGGGTAT TACAGGTATC 
TCTGGAGATC CTGGTCTGCC TGGGCAAGAT GGACGACCAG GACTACCAGG ACCCCCAGGC 
CCCAAAGGTG ATGCTGGTCT CCCAGGAATT CCAGGTTCTC CAGGAGCCAC AGGATTAAAG 
GGGGCCATGG GAGAGATGGG ATTGCCAGGA GTTCCTGGAG TCAAAGGAAA TTCCGGCCAA 
CCTGGCCAAC CTGGCCAGCC AGGATCCCCA GGTGGACCAG GATTCCCAGG ACCTAAAGGT 
GAAGCTGGTG TACCAGGATA CGGATTACCT GGAAATCCCG GACCTAAGGG TGAACCAGGC 
ACTCCAGGCT TTCCTGGACC CGCTGGCTTG AAAGGCCAGC CTGGCTTTCC AGGACCTCAA 
GGCTTGACTG GTAGCCCTGG ACCAAAGGGT GATCCGGGAC TCCCAGGATT TCAAGGTGCA 
CCTGGCAATC CTGGCCCAAA AGGTTTGGAT GGTGCTCCAG GCCCTGCAGG TTTAAATGGA 
GCACCTGGTA GGCCAGGAGA TCCCGGACGA TCTGGATTAC CTGGTATCCC TGGAGATAAA 
GGTCTTGCTG GTCGTGATGG TATTCCAGGA CCAGCAGGTC AAAAGGGTGA ACCAGGTGCT 
CCAGGCCTTG GTGGCCCAGG CTTGCCTGGT CAACCTGGTT TGAAAGGCGC TAAAGGAGAT 
CCTGGTTTTC CGGGTCCTGC CGGTAGTGCT GGATTTCCTG GTCCAAAGGG TGATGCAGGA 
TTTCCTGGCT TGCCTGGTCA GATTGGTTCT GTAGGACCAC CTGGGCCTCC AGGCTTGCCA 
CTGCAGGGAC CTAAAGGTAT CCAAGGTCCA CCTGGTCCCC CCGGAAGAGC TGGTCCTCCT 
GGCCTTGAGG GTCCACGTGG ACCTCCAGGT AGCGGAGGAG TGAAGGGTGA GAAGGGTTTG 
CCTGGAGTTC CTGGGACGCC TGGAGAAGCA GGACAGAAAG GAGATTATGG TTCTCCTGGG 
TTTCCGGGCT CCCCAGGGAG TCCAGGACCA AATGGCCTCA AAGGAGATCC TGGACCTCAA 
GGTGTTCCTG GTTTTCCAGG ATCTAAAGGA GTGTCTGGTC CACCTGGTAT TTCTGGCCTC 
CCTGGAGCAC GTGGTGATCC TGGAGATAAA GGACCTCCCG GACCCCCAGG TTATGCTGCA 
GAGCCTATTG TCTTGAAGGG CGAGCGTGGC CCACCAGGAC CTCCAGGTAA TCCAGGCGGC 
AGAGGACAAG CCGGACCCAT CGGATTGCAG GGACTCCCAG GTTCGCCTGG CAGCTCTGGA 
GACCCAGGAC CAATGGGACC TCCAGGATTT AATGGCCCAC CTGGAAGGAA GGGAGACACA 
GGTCCAGCTG GACAGCCAGG TCAGCGTGGT TACCCCGGCC CTCCAGGTCC AGATGGGGCT 
CCGGGGCCTC CAGGTATCAC GGGAACTTTG TCTGGTGCAC ACGGTTTTCT GATCACACGC 
CACAGTCAGA CCATAGAAGT GCCATCGTGT CCTGATGGCA CAAGCCCAAT CTACGAGGGC 
TACTCTTTAC TCTACGTGCA AGGCAACGAG CGGGCACACG GTCAAGATCT GGGTACGGCT 
GGCAGCTGTC TACGGCGTTT CAGCACGATG CCTTTCATGT TCTGCAACAT CAACAATGTC 
TGTAACTTCG CTTCCCGTAA TGATTACTCA TACTGGCTGT CCACACCACA GCCCATGCCC 
ATGACCATGG CACCCATCAC TGGAGAGAAC ATCAAACCTT ACATCAGCCG GTGTTCTGTG 
TGTGAAGCTC CAGCAATGGT GATTGCTGTA CACAGTCAGA CCATTCAGTT GCCAGCGTGC 
CCTGAAAACT GGGATACCCT CTGGATCGGT TACTCCTTCA TGATGCACAC CAGTGCAGGT 
GCTGAGGGTT CAGGTCAGGC TCTGGCCTCT CCGGGCTCCT GTCTGGAGGA GTTTCGCTCC 
GCTCCCTTCA TTGAGTGCCA CGGACGAGGA ACCTGCAATT ATTACGGCAA CTCCTACAGC 
TTCTGGCTGG CCACTGTGGA CGTGAACGAA ATGTTCAGAA AGCCGCAGTC AGAGACTCTA 
AAAGCTGGAA ACTTGAGGAC CCGCGTGAGC CGCTGTGTGG TGTGTATGAA GAGGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a5 ( XM_021477401.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333076.1 Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), transcript variant X1, mRNA.

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CloneID	ODa143202
Clone ID Related Accession (Same CDS sequence)	XM_021477401.1
Accession Version	XM_021477401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTAA AAAGTCTCGG CGCGGTGTTG CTGCTTGTGC TCACTCTGAG TGCGGGAGTA 
GAGAAATCCG AGAGTGCGGC ATGTCATGGT TGTGGGACTG GATCAAAATG TGACTGCAGT 
TCAGTGAAAG GAGTGAAGGG AGATCGAGGA CTTCCGGGTT TAAAGGGTCA GCCGGGTGTT 
CCAGGTTTCC CAGGACCAGA GGGACCAATG GGACCTCGTG GAGAGAAAGG TGATGACGGA 
CCTCAAGGGT CGATAGGCCC CAAAGGAATA AGAGGACCTC CAGGATTACC TGGATTTTCA 
GGAACACCAG GAATCCCAGG TCTTCCAGGA CAGGATGGTG CTCCAGGACC CAGAGGAATT 
CCAGGCTGTA ATGGAACTAA AGGTGAAAGA GGATTCCCTG GAAATCCAGG TCGCTCAGGT 
TTAGATGGTC AACCAGGTCC ACCAGGTCTT ATAGGACAAA AAGGAGAACC TGGGGAAGTC 
ATCGACATTA ACCGCATTTC AGAAAATGGA CTTCCAGGCT TTAATGGCTT TACAGGCAGA 
AGGGGTGATC CCGGACCCCC AGGTCTTCAA GGGCCTATCG GTCCTCCTGG TCCAAAAGGG 
TTTGAGGGTC GCCCAGGCCC TCCTGGTCCA CCGGGACCAA AAGGAAACAT GGGATTGAAT 
TTCCAAGGAC CCAAAGGAGA GAAGGGTGAC CGTGGATTAA AAGGACCTCC AGGGCCACCA 
GGACAAGTTG GTGAAGTGTC AGGTTCACCA GATACAGTCA TTCAACAAGG AGAAAAGGGT 
GATCGTGGCA TACCTGGAGA TACTGGTTTC CCAGGGCCCC CTGGACCCCC TGGTCCACAA 
GGAGGTCAGA AAGGAGAGAA AGGAGAACCT GGGGAGGCTG GAAAGAGGGG AAAACCTGGC 
AAAGATGGTG AACCAGGTCT TCTTGGATAT CCGGGTGATA AAGGAGACCA AGGGCCTCCT 
GGTCTGCCTG GACGAGATGG TTTTAGAGGT GAGAAAGGTG ATCAAGGGCC ACCTGGACCT 
CCCTCCTACC GGGGTGATCA GTTCACAGGT GTAGGTGGAA TAGGACCAAA AGGAGACCGT 
GGTGATCCAG GACCCTCTGG TATTAAAGGA GACAGAGGAC TACCAGGTAT TCAGGGGCCA 
CCTGGTCCAC AGGGTCCTCC AGGAATTGGA GGTCCAGGAC CTCCCGGTTC ACAAGGATTG 
CCTGGAGAAC GAGGAGAAAA GGGTGATGAG GGACAGCCTG GAATCTCACT TCCTGGACCA 
CCCGGGAGAC CCGGACCAAC AGGACCACAA GGAATACAAG GACCACCTGG CCCCCCAGGA 
CCATTCAACA GTGATGTAGA ATGTAATCGT GGTCTTCCTG GTCCTCCAGG CCTGCAGGGG 
GAGCGTGGGT TTACTGGAGA TAGTGGGCAG AAAGGTCAAA AAGGCGAGAC TTGTGTGAAT 
TGTTTCTCCA ATGGAAACCC TGTCCCAGGA CCCCAAGGTT TTCCAGGTCC TCCTGGAAGT 
CCAGGTGCTC CTGGGGCGTC TGGATCTAAA GGAGACAGGG GATATCCGGG AGGACCAGGG 
CTTATGGGCC CACCTGGAAT ACCTGGACCT CAGGGTTCCC CTGGACTCCC TGGAGAGAAG 
GGTGACCCAG GCGATACCCT CTCTGTTCTT GGCGTGAAGG GAGATAAAGG AGACACAGGT 
TTTCCTGGCT CTCCAGGTCT GCCGGGACTC GAAGGAAGAC CTGGACGTGA TGGAGTGCCT 
GGTCCACCTG GCCTCAGAGG ACCACCTGGT TTGGGACTGA AAGGTACACA AGGTCCCAAC 
GGTGAACCTG GTTTGCCAGG ACCTCCTGGA GACAGAGGTT TACCAGGTTC CCCAGGATTT 
GGTCCTCAAG GACCTTCTGG AGAAAAAGGT ATTCAGGGTG TTTCAGGAAG ACCAGGGGCA 
CCTGGACTAC CAGGTCAGAA AGGTGAACCT GGGATGACTG TGCCAGAAGT TGGTGAACGT 
GGACCCCCAG GACAGGATGG AGATCCTGGT TCACAAGGAC GTCCAGGTGA TTCAGGCCCA 
CCTGGACAGC CTGGGTTCCC AGGTTTGCCT GGATCCAAAG GAGAGCCTGG TCTTCCTGGT 
ATTGGTTTGC CAGGACCTCC TGGTGCTAAA GGATTTCCTG GTATTGCTGG TTCCCCTGGT 
GGACCTGGCA TTCCTGGACG ACCAGGTTTA GATGGACTGC CTGGTCAGCC TGGATTACCT 
GGATCCAAAG GAGATCCTGG TTATGGTCTG CCTGGTCCTC CAGGTCCCAC AGGATCACCA 
GGCATTAAAG GTGGACCCGG TCCGAAAGGT GATTCTGGTT TCCCTGGAAG CCCTGGTCAA 
CCAGGCCGCC CAGGTCTCGA TGGGGCTCCT GGACCTAAAG GTGATGCTGG TTTTCCTGGT 
GGTCCTGGTC CTCGTGGCCC CCCTGGGGCT CCTGCTTTTG GTCTTCAGGG CCCTCCTGGT 
CCTCCTGGAG CTCCAGGCTC TATTGGATCT CCTGGAGTAC CGGGGGCGAA TGGAGAAAAA 
GGAGACAGAG GCCCTCCAGG TCTGAGTACT CCAGGCTTCC AGGGTGATAG AGGAATTTCT 
GGACTTCCTG GACCTCCTGG TCCAGTTGGT CCACCTGGAG TTCCAGGCCG ACCTGGACAA 
GATGGCCTAC CAGGACTCCC TGGATCTAAG GGAGAAATGG GCTCAATGGG GCCTCCCGGA 
TCAAAAGGGA ACCCCGGCAA CCCTGGAGCT CCCGGCTTTC CAGGACCAAA AGGAGATGAT 
GGTGTCCCAG GACAGTCAGG AAATCCAGGT TTTCCAGGGT TAAAAGGAGA CAAAGGTGAA 
ACAGGCATTC AAGGCCCGCC TGGCATAACC ACATCTACAA GTGTACTGAA AGGACAAAAA 
GGAGAATCTG GTGTTCCAGG TTTCAGTGGA CCGCCAGGAC CAAAGGGTAT TACAGGTATC 
TCTGGAGATC CTGGTCTGCC TGGGCAAGAT GGACGACCAG GACTACCAGG ACCCCCAGGC 
CCCAAAGGTG ATGCTGGTCT CCCAGGAATT CCAGGTTCTC CAGGAGCCAC AGGATTAAAG 
GGGGCCATGG GAGAGATGGG ATTGCCAGGA GTTCCTGGAG TCAAAGGAAA TTCCGGCCAA 
CCTGGCCAAC CTGGCCAGCC AGGATCCCCA GGTGGACCAG GATTCCCAGG ACCTAAAGGT 
GAAGCTGGTG TACCAGGATA CGGATTACCT GGAAATCCCG GACCTAAGGG TGAACCAGGC 
ACTCCAGGCT TTCCTGGACC CGCTGGCTTG AAAGGCCAGC CTGGCTTTCC AGGACCTCAA 
GGCTTGACTG GTAGCCCTGG ACCAAAGGGT GATCCGGGAC TCCCAGGATT TCAAGGTGCA 
CCTGGCAATC CTGGCCCAAA AGGTTTGGAT GGTGCTCCAG GCCCTGCAGG TTTAAATGGA 
GCACCTGGTA GGCCAGGAGA TCCCGGACGA TCTGGATTAC CTGGTATCCC TGGAGATAAA 
GGTCTTGCTG GTCGTGATGG TATTCCAGGA CCAGCAGGTC AAAAGGGTGA ACCAGGTGCT 
CCAGGCCTTG GTGGCCCAGG CTTGCCTGGT CAACCTGGTT TGAAAGGCGC TAAAGGAGAT 
CCTGGTTTTC CGGGTCCTGC CGGTAGTGCT GGATTTCCTG GTCCAAAGGG TGATGCAGGA 
TTTCCTGGCT TGCCTGGTCA GATTGGTTCT GTAGGACCAC CTGGGCCTCC AGGCTTGCCA 
CTGCAGGGAC CTAAAGGTAT CCAAGGTCCA CCTGGTCCCC CCGGAAGAGC TGGCCCGGCA 
GGTCCCGCAG GTCCTCCTGG CCTTGAGGGT CCACGTGGAC CTCCAGGTAG CGGAGGAGTG 
AAGGGTGAGA AGGGTTTGCC TGGAGTTCCT GGGACGCCTG GAGAAGCAGG ACAGAAAGGA 
GATTATGGTT CTCCTGGGTT TCCGGGCTCC CCAGGGAGTC CAGGACCAAA TGGCCTCAAA 
GGAGATCCTG GACCTCAAGG TGTTCCTGGT TTTCCAGGAT CTAAAGGAGT GTCTGGTCCA 
CCTGGTATTT CTGGCCTCCC TGGAGCACGT GGTGATCCTG GAGATAAAGG ACCTCCCGGA 
CCCCCAGGTT ATGCTGCAGA GCCTATTGTC TTGAAGGGCG AGCGTGGCCC ACCAGGACCT 
CCAGGTAATC CAGGCGGCAG AGGACAAGCC GGACCCATCG GATTGCAGGG ACTCCCAGGT 
TCGCCTGGCA GCTCTGGAGA CCCAGGACCA ATGGGACCTC CAGGATTTAA TGGCCCACCT 
GGAAGGAAGG GAGACACAGG TCCAGCTGGA CAGCCAGGTC AGCGTGGTTA CCCCGGCCCT 
CCAGGTCCAG ATGGGGCTCC GGGGCCTCCA GGTATCACGG GAACTTTGTC TGGTGCACAC 
GGTTTTCTGA TCACACGCCA CAGTCAGACC ATAGAAGTGC CATCGTGTCC TGATGGCACA 
AGCCCAATCT ACGAGGGCTA CTCTTTACTC TACGTGCAAG GCAACGAGCG GGCACACGGT 
CAAGATCTGG GTACGGCTGG CAGCTGTCTA CGGCGTTTCA GCACGATGCC TTTCATGTTC 
TGCAACATCA ACAATGTCTG TAACTTCGCT TCCCGTAATG ATTACTCATA CTGGCTGTCC 
ACACCACAGC CCATGCCCAT GACCATGGCA CCCATCACTG GAGAGAACAT CAAACCTTAC 
ATCAGCCGGT GTTCTGTGTG TGAAGCTCCA GCAATGGTGA TTGCTGTACA CAGTCAGACC 
ATTCAGTTGC CAGCGTGCCC TGAAAACTGG GATACCCTCT GGATCGGTTA CTCCTTCATG 
ATGCACACCA GTGCAGGTGC TGAGGGTTCA GGTCAGGCTC TGGCCTCTCC GGGCTCCTGT 
CTGGAGGAGT TTCGCTCCGC TCCCTTCATT GAGTGCCACG GACGAGGAAC CTGCAATTAT 
TACGGCAACT CCTACAGCTT CTGGCTGGCC ACTGTGGACG TGAACGAAAT GTTCAGAAAG 
CCGCAGTCAG AGACTCTAAA AGCTGGAAAC TTGAGGACCC GCGTGAGCCG CTGTGTGGTG 
TGTATGAAGA GGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333076.1
CDS	189..5246
Translation	MNLKSLGAVLLLVLTLSAGVEKSESAACHGCGTGSKCDCSSVKGVKGDRGLPGLKGQPGVPGFPGPEGPMGPRGEKGDDGPQGSIGPKGIRGPPGLPGFSGTPGIPGLPGQDGAPGPRGIPGCNGTKGERGFPGNPGRSGLDGQPGPPGLIGQKGEPGEVIDINRISENGLPGFNGFTGRRGDPGPPGLQGPIGPPGPKGFEGRPGPPGPPGPKGNMGLNFQGPKGEKGDRGLKGPPGPPGQVGEVSGSPDTVIQQGEKGDRGIPGDTGFPGPPGPPGPQGGQKGEKGEPGEAGKRGKPGKDGEPGLLGYPGDKGDQGPPGLPGRDGFRGEKGDQGPPGPPSYRGDQFTGVGGIGPKGDRGDPGPSGIKGDRGLPGIQGPPGPQGPPGIGGPGPPGSQGLPGERGEKGDEGQPGISLPGPPGRPGPTGPQGIQGPPGPPGPFNSDVECNRGLPGPPGLQGERGFTGDSGQKGQKGETCVNCFSNGNPVPGPQGFPGPPGSPGAPGASGSKGDRGYPGGPGLMGPPGIPGPQGSPGLPGEKGDPGDTLSVLGVKGDKGDTGFPGSPGLPGLEGRPGRDGVPGPPGLRGPPGLGLKGTQGPNGEPGLPGPPGDRGLPGSPGFGPQGPSGEKGIQGVSGRPGAPGLPGQKGEPGMTVPEVGERGPPGQDGDPGSQGRPGDSGPPGQPGFPGLPGSKGEPGLPGIGLPGPPGAKGFPGIAGSPGGPGIPGRPGLDGLPGQPGLPGSKGDPGYGLPGPPGPTGSPGIKGGPGPKGDSGFPGSPGQPGRPGLDGAPGPKGDAGFPGGPGPRGPPGAPAFGLQGPPGPPGAPGSIGSPGVPGANGEKGDRGPPGLSTPGFQGDRGISGLPGPPGPVGPPGVPGRPGQDGLPGLPGSKGEMGSMGPPGSKGNPGNPGAPGFPGPKGDDGVPGQSGNPGFPGLKGDKGETGIQGPPGITTSTSVLKGQKGESGVPGFSGPPGPKGITGISGDPGLPGQDGRPGLPGPPGPKGDAGLPGIPGSPGATGLKGAMGEMGLPGVPGVKGNSGQPGQPGQPGSPGGPGFPGPKGEAGVPGYGLPGNPGPKGEPGTPGFPGPAGLKGQPGFPGPQGLTGSPGPKGDPGLPGFQGAPGNPGPKGLDGAPGPAGLNGAPGRPGDPGRSGLPGIPGDKGLAGRDGIPGPAGQKGEPGAPGLGGPGLPGQPGLKGAKGDPGFPGPAGSAGFPGPKGDAGFPGLPGQIGSVGPPGPPGLPLQGPKGIQGPPGPPGRAGPAGPAGPPGLEGPRGPPGSGGVKGEKGLPGVPGTPGEAGQKGDYGSPGFPGSPGSPGPNGLKGDPGPQGVPGFPGSKGVSGPPGISGLPGARGDPGDKGPPGPPGYAAEPIVLKGERGPPGPPGNPGGRGQAGPIGLQGLPGSPGSSGDPGPMGPPGFNGPPGRKGDTGPAGQPGQRGYPGPPGPDGAPGPPGITGTLSGAHGFLITRHSQTIEVPSCPDGTSPIYEGYSLLYVQGNERAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPQPMPMTMAPITGENIKPYISRCSVCEAPAMVIAVHSQTIQLPACPENWDTLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYGNSYSFWLATVDVNEMFRKPQSETLKAGNLRTRVSRCVVCMKRT

Target ORF information:

RefSeq Version	XM_021477401.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IV, alpha 5 (Alport syndrome) (col4a5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477401.1

ORF Insert Sequence:

ATGAACTTAA AAAGTCTCGG CGCGGTGTTG CTGCTTGTGC TCACTCTGAG TGCGGGAGTA 
GAGAAATCCG AGAGTGCGGC ATGTCATGGT TGTGGGACTG GATCAAAATG TGACTGCAGT 
TCAGTGAAAG GAGTGAAGGG AGATCGAGGA CTTCCGGGTT TAAAGGGTCA GCCGGGTGTT 
CCAGGTTTCC CAGGACCAGA GGGACCAATG GGACCTCGTG GAGAGAAAGG TGATGACGGA 
CCTCAAGGGT CGATAGGCCC CAAAGGAATA AGAGGACCTC CAGGATTACC TGGATTTTCA 
GGAACACCAG GAATCCCAGG TCTTCCAGGA CAGGATGGTG CTCCAGGACC CAGAGGAATT 
CCAGGCTGTA ATGGAACTAA AGGTGAAAGA GGATTCCCTG GAAATCCAGG TCGCTCAGGT 
TTAGATGGTC AACCAGGTCC ACCAGGTCTT ATAGGACAAA AAGGAGAACC TGGGGAAGTC 
ATCGACATTA ACCGCATTTC AGAAAATGGA CTTCCAGGCT TTAATGGCTT TACAGGCAGA 
AGGGGTGATC CCGGACCCCC AGGTCTTCAA GGGCCTATCG GTCCTCCTGG TCCAAAAGGG 
TTTGAGGGTC GCCCAGGCCC TCCTGGTCCA CCGGGACCAA AAGGAAACAT GGGATTGAAT 
TTCCAAGGAC CCAAAGGAGA GAAGGGTGAC CGTGGATTAA AAGGACCTCC AGGGCCACCA 
GGACAAGTTG GTGAAGTGTC AGGTTCACCA GATACAGTCA TTCAACAAGG AGAAAAGGGT 
GATCGTGGCA TACCTGGAGA TACTGGTTTC CCAGGGCCCC CTGGACCCCC TGGTCCACAA 
GGAGGTCAGA AAGGAGAGAA AGGAGAACCT GGGGAGGCTG GAAAGAGGGG AAAACCTGGC 
AAAGATGGTG AACCAGGTCT TCTTGGATAT CCGGGTGATA AAGGAGACCA AGGGCCTCCT 
GGTCTGCCTG GACGAGATGG TTTTAGAGGT GAGAAAGGTG ATCAAGGGCC ACCTGGACCT 
CCCTCCTACC GGGGTGATCA GTTCACAGGT GTAGGTGGAA TAGGACCAAA AGGAGACCGT 
GGTGATCCAG GACCCTCTGG TATTAAAGGA GACAGAGGAC TACCAGGTAT TCAGGGGCCA 
CCTGGTCCAC AGGGTCCTCC AGGAATTGGA GGTCCAGGAC CTCCCGGTTC ACAAGGATTG 
CCTGGAGAAC GAGGAGAAAA GGGTGATGAG GGACAGCCTG GAATCTCACT TCCTGGACCA 
CCCGGGAGAC CCGGACCAAC AGGACCACAA GGAATACAAG GACCACCTGG CCCCCCAGGA 
CCATTCAACA GTGATGTAGA ATGTAATCGT GGTCTTCCTG GTCCTCCAGG CCTGCAGGGG 
GAGCGTGGGT TTACTGGAGA TAGTGGGCAG AAAGGTCAAA AAGGCGAGAC TTGTGTGAAT 
TGTTTCTCCA ATGGAAACCC TGTCCCAGGA CCCCAAGGTT TTCCAGGTCC TCCTGGAAGT 
CCAGGTGCTC CTGGGGCGTC TGGATCTAAA GGAGACAGGG GATATCCGGG AGGACCAGGG 
CTTATGGGCC CACCTGGAAT ACCTGGACCT CAGGGTTCCC CTGGACTCCC TGGAGAGAAG 
GGTGACCCAG GCGATACCCT CTCTGTTCTT GGCGTGAAGG GAGATAAAGG AGACACAGGT 
TTTCCTGGCT CTCCAGGTCT GCCGGGACTC GAAGGAAGAC CTGGACGTGA TGGAGTGCCT 
GGTCCACCTG GCCTCAGAGG ACCACCTGGT TTGGGACTGA AAGGTACACA AGGTCCCAAC 
GGTGAACCTG GTTTGCCAGG ACCTCCTGGA GACAGAGGTT TACCAGGTTC CCCAGGATTT 
GGTCCTCAAG GACCTTCTGG AGAAAAAGGT ATTCAGGGTG TTTCAGGAAG ACCAGGGGCA 
CCTGGACTAC CAGGTCAGAA AGGTGAACCT GGGATGACTG TGCCAGAAGT TGGTGAACGT 
GGACCCCCAG GACAGGATGG AGATCCTGGT TCACAAGGAC GTCCAGGTGA TTCAGGCCCA 
CCTGGACAGC CTGGGTTCCC AGGTTTGCCT GGATCCAAAG GAGAGCCTGG TCTTCCTGGT 
ATTGGTTTGC CAGGACCTCC TGGTGCTAAA GGATTTCCTG GTATTGCTGG TTCCCCTGGT 
GGACCTGGCA TTCCTGGACG ACCAGGTTTA GATGGACTGC CTGGTCAGCC TGGATTACCT 
GGATCCAAAG GAGATCCTGG TTATGGTCTG CCTGGTCCTC CAGGTCCCAC AGGATCACCA 
GGCATTAAAG GTGGACCCGG TCCGAAAGGT GATTCTGGTT TCCCTGGAAG CCCTGGTCAA 
CCAGGCCGCC CAGGTCTCGA TGGGGCTCCT GGACCTAAAG GTGATGCTGG TTTTCCTGGT 
GGTCCTGGTC CTCGTGGCCC CCCTGGGGCT CCTGCTTTTG GTCTTCAGGG CCCTCCTGGT 
CCTCCTGGAG CTCCAGGCTC TATTGGATCT CCTGGAGTAC CGGGGGCGAA TGGAGAAAAA 
GGAGACAGAG GCCCTCCAGG TCTGAGTACT CCAGGCTTCC AGGGTGATAG AGGAATTTCT 
GGACTTCCTG GACCTCCTGG TCCAGTTGGT CCACCTGGAG TTCCAGGCCG ACCTGGACAA 
GATGGCCTAC CAGGACTCCC TGGATCTAAG GGAGAAATGG GCTCAATGGG GCCTCCCGGA 
TCAAAAGGGA ACCCCGGCAA CCCTGGAGCT CCCGGCTTTC CAGGACCAAA AGGAGATGAT 
GGTGTCCCAG GACAGTCAGG AAATCCAGGT TTTCCAGGGT TAAAAGGAGA CAAAGGTGAA 
ACAGGCATTC AAGGCCCGCC TGGCATAACC ACATCTACAA GTGTACTGAA AGGACAAAAA 
GGAGAATCTG GTGTTCCAGG TTTCAGTGGA CCGCCAGGAC CAAAGGGTAT TACAGGTATC 
TCTGGAGATC CTGGTCTGCC TGGGCAAGAT GGACGACCAG GACTACCAGG ACCCCCAGGC 
CCCAAAGGTG ATGCTGGTCT CCCAGGAATT CCAGGTTCTC CAGGAGCCAC AGGATTAAAG 
GGGGCCATGG GAGAGATGGG ATTGCCAGGA GTTCCTGGAG TCAAAGGAAA TTCCGGCCAA 
CCTGGCCAAC CTGGCCAGCC AGGATCCCCA GGTGGACCAG GATTCCCAGG ACCTAAAGGT 
GAAGCTGGTG TACCAGGATA CGGATTACCT GGAAATCCCG GACCTAAGGG TGAACCAGGC 
ACTCCAGGCT TTCCTGGACC CGCTGGCTTG AAAGGCCAGC CTGGCTTTCC AGGACCTCAA 
GGCTTGACTG GTAGCCCTGG ACCAAAGGGT GATCCGGGAC TCCCAGGATT TCAAGGTGCA 
CCTGGCAATC CTGGCCCAAA AGGTTTGGAT GGTGCTCCAG GCCCTGCAGG TTTAAATGGA 
GCACCTGGTA GGCCAGGAGA TCCCGGACGA TCTGGATTAC CTGGTATCCC TGGAGATAAA 
GGTCTTGCTG GTCGTGATGG TATTCCAGGA CCAGCAGGTC AAAAGGGTGA ACCAGGTGCT 
CCAGGCCTTG GTGGCCCAGG CTTGCCTGGT CAACCTGGTT TGAAAGGCGC TAAAGGAGAT 
CCTGGTTTTC CGGGTCCTGC CGGTAGTGCT GGATTTCCTG GTCCAAAGGG TGATGCAGGA 
TTTCCTGGCT TGCCTGGTCA GATTGGTTCT GTAGGACCAC CTGGGCCTCC AGGCTTGCCA 
CTGCAGGGAC CTAAAGGTAT CCAAGGTCCA CCTGGTCCCC CCGGAAGAGC TGGCCCGGCA 
GGTCCCGCAG GTCCTCCTGG CCTTGAGGGT CCACGTGGAC CTCCAGGTAG CGGAGGAGTG 
AAGGGTGAGA AGGGTTTGCC TGGAGTTCCT GGGACGCCTG GAGAAGCAGG ACAGAAAGGA 
GATTATGGTT CTCCTGGGTT TCCGGGCTCC CCAGGGAGTC CAGGACCAAA TGGCCTCAAA 
GGAGATCCTG GACCTCAAGG TGTTCCTGGT TTTCCAGGAT CTAAAGGAGT GTCTGGTCCA 
CCTGGTATTT CTGGCCTCCC TGGAGCACGT GGTGATCCTG GAGATAAAGG ACCTCCCGGA 
CCCCCAGGTT ATGCTGCAGA GCCTATTGTC TTGAAGGGCG AGCGTGGCCC ACCAGGACCT 
CCAGGTAATC CAGGCGGCAG AGGACAAGCC GGACCCATCG GATTGCAGGG ACTCCCAGGT 
TCGCCTGGCA GCTCTGGAGA CCCAGGACCA ATGGGACCTC CAGGATTTAA TGGCCCACCT 
GGAAGGAAGG GAGACACAGG TCCAGCTGGA CAGCCAGGTC AGCGTGGTTA CCCCGGCCCT 
CCAGGTCCAG ATGGGGCTCC GGGGCCTCCA GGTATCACGG GAACTTTGTC TGGTGCACAC 
GGTTTTCTGA TCACACGCCA CAGTCAGACC ATAGAAGTGC CATCGTGTCC TGATGGCACA 
AGCCCAATCT ACGAGGGCTA CTCTTTACTC TACGTGCAAG GCAACGAGCG GGCACACGGT 
CAAGATCTGG GTACGGCTGG CAGCTGTCTA CGGCGTTTCA GCACGATGCC TTTCATGTTC 
TGCAACATCA ACAATGTCTG TAACTTCGCT TCCCGTAATG ATTACTCATA CTGGCTGTCC 
ACACCACAGC CCATGCCCAT GACCATGGCA CCCATCACTG GAGAGAACAT CAAACCTTAC 
ATCAGCCGGT GTTCTGTGTG TGAAGCTCCA GCAATGGTGA TTGCTGTACA CAGTCAGACC 
ATTCAGTTGC CAGCGTGCCC TGAAAACTGG GATACCCTCT GGATCGGTTA CTCCTTCATG 
ATGCACACCA GTGCAGGTGC TGAGGGTTCA GGTCAGGCTC TGGCCTCTCC GGGCTCCTGT 
CTGGAGGAGT TTCGCTCCGC TCCCTTCATT GAGTGCCACG GACGAGGAAC CTGCAATTAT 
TACGGCAACT CCTACAGCTT CTGGCTGGCC ACTGTGGACG TGAACGAAAT GTTCAGAAAG 
CCGCAGTCAG AGACTCTAAA AGCTGGAAAC TTGAGGACCC GCGTGAGCCG CTGTGTGGTG 
TGTATGAAGA GGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fishing for collagen function: About development, regeneration and disease.
Seminars in cell & developmental biology89100-108(2019 05)
Bretaud S,Nauroy P,Malbouyres M,Ruggiero F

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Cell reports27(5)1567-1578.e5(2019 04)
Xue Y,Liu D,Cui G,Ding Y,Ai D,Gao S,Zhang Y,Suo S,Wang X,Lv P,Zhou C,Li Y,Chen X,Peng G,Jing N,Han JJ,Liu F

Testicular transcriptome alterations in zebrafish (Danio rerio) exposure to 17β-estradiol.
Chemosphere21814-25(2019 Mar)
Zheng Y,Yuan J,Meng S,Chen J,Gu Z

Identification of p53-target genes in Danio rerio.
Scientific reports632474(2016 09)
Mandriani B,Castellana S,Rinaldi C,Manzoni M,Venuto S,Rodriguez-Aznar E,Galceran J,Nieto MA,Borsani G,Monti E,Mazza T,Merla G,Micale L

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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col4a5 cDNA ORF clone, Danio rerio(zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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