krit1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	krit1
Entrez Gene ID	321836
Full Name	KRIT1, ankyrin repeat containing
Synonyms	fb36f07,san,santa,wu:fb36f07,zgc:63585
General protein information	Preferred Names KRIT1, ankyrin repeat containing Names krev interaction trapped protein 1 ccm1 cerebral cavernous malformations 1 protein homolog krev interaction trapped 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001317001.1	NP_001303930.1	krev interaction trapped protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KRIT1	XP_001166722.1
Rattus norvegicus (Norway rat)	Krit1	NP_001102088.1
Danio rerio (zebrafish)	krit1	NP_955866.1
Bos taurus (cattle)	KRIT1	NP_001001166.1
Mus musculus (house mouse)	Krit1	NP_109600.2
Macaca mulatta (Rhesus monkey)	KRIT1	XP_001101416.1
Gallus gallus (chicken)	KRIT1	NP_001026144.1
Xenopus tropicalis (tropical clawed frog)	krit1	NP_001121439.1
Canis lupus familiaris (dog)	KRIT1	XP_539416.2
Homo sapiens (human)	KRIT1	NP_004903.2
Caenorhabditis elegans (roundworm)	kri-1	NP_001021863.1

Gene Ontology
Bibliography

Related articles in PubMed

The CCM1-CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity.
Lisowska J, Rödel CJ, Manet S, Miroshnikova YA, Boyault C, Planus E, De Mets R, Lee HH, Destaing O, Mertani H, Boulday G, Tournier-Lasserve E, Balland M, Abdelilah-Seyfried S, Albiges-Rizo C, Faurobert E
Journal of cell science131(15)(2018 08)

Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis.
Donat S, Lourenço M, Paolini A, Otten C, Renz M, Abdelilah-Seyfried S
eLife7(2018 02)

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Gistelinck C, Gioia R, Gagliardi A, Tonelli F, Marchese L, Bianchi L, Landi C, Bini L, Huysseune A, Witten PE, Staes A, Gevaert K, De Rocker N, Menten B, Malfait F, Leikin S, Carra S, Tenni R, Rossi A, De Paepe A, Coucke P, Willaert A, Forlino A
Scientific reports621540(2016 Feb)

The cerebral cavernous malformation proteins CCM2L and CCM2 prevent the activation of the MAP kinase MEKK3.
Cullere X, Plovie E, Bennett PM, MacRae CA, Mayadas TN
Proceedings of the National Academy of Sciences of the United States of America112(46)14284-9(2015 Nov)

Lack of CCM1 induces hypersprouting and impairs response to flow.
Mleynek TM, Chan AC, Redd M, Gibson CC, Davis CT, Shi DS, Chen T, Carter KL, Ling J, Blanco R, Gerhardt H, Whitehead K, Li DY
Human molecular genetics23(23)6223-34(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following krit1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the krit1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa24486	NM_001317001.1 Latest version!	Danio rerio KRIT1 ankyrin repeat containing (krit1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

krit1 ( NM_001317001.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001303930.1 Danio rerio KRIT1 ankyrin repeat containing (krit1), mRNA.

Price & Availability↑

CloneID	ODa24486
Clone ID Related Accession (Same CDS sequence)	NM_001317001.1
Accession Version	NM_001317001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571673600000
Organism	Danio rerio(zebrafish)
Product	krev interaction trapped protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EB920540.1 and CR855331.14. On Nov 6, 2015 this sequence version replaced XM_005159791.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ677877.1, GDQH01009592.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATGGGAA ACCAAGAGCT AGAGGAGGTG TTTGTTGCTG TTATCCGACC AAAAAACACA 
ACCAGCCTCA ACTCCAAAGA ATACCGTGCT AAATCCTATG AGATTTTACT TATCGAAGTT 
CCCCTGGAGG GAAAAGAGAA AAAGCGGAAG AAAGTGCTGC TTGGGACTAA AATCCACGCC 
GACAGTGACC GGACAAAGTC AATTCTGGAG TTTGTGGATG AAACCACCAA GCCCATATCC 
AATAACCAGG GCATAATAGG GAAGCGTGTC GTGCACATGA GGAAGTTCCT GTTAGATGGA 
GACAGTGGAG GAAAAGAGGC CTCGTTATTT ATTGTGCCAA TTAATGTCAA AGACAACAGC 
AAGTCCGTGC ATCACCCTGG GAGCCCCAGT TTCTACTGCC TTCAGGACAT CATGCGTGTG 
TGTAGTGAAA CCAGTGCTCA TTTCTCCTCC AGCACCTCAA AGATGCTGCT CGCTCTCGAC 
AAGTGGTTAG CGGAGCAGCA CACTGTGCCT CACGCTATTC CTGCTCTGTT CCGGCCGGCT 
CCGGTGGACC GTGTGAAAAC TAACGTGAGT AACCCGGCGT ACGCAGTGGA GAAGCAGACA 
GACGGGACGC TGCACATGGG CTACACGGCT CTGGAGATCA AGAGTAAACT GATGTCTCTG 
GAGAAAGCAG ATCTGTGCAT CCAGAACCCG CTGTACGGCT CAGATCTGCA GTACACCAAC 
AGAGTGGACA AAGTCATCAT CAACCCTTAC TTTGGCTTGG GAGCTCCAGA TTACTCCAAA 
ATCCAGATTC CAAAGAGAGA CAAATGGCAG CACAGCATGA CCAGCGTCAC CGAAGACAAA 
GAGCGCCAGT GGGTGGATGA TTTCCCTCTG CACCGAAGCG CCTGTGAGGG AGACACTGAG 
CTTTTGTCTA AATTGCTGGA TGGCGGATTC TCAGTCAAAC AGCTGGACAG CGACCATTGG 
GCACCCATTC ATTATGCATG CTGGCATGGG AAGGTGGAGG CCACTAAACT GCTGCTGGAG 
AAAGGCAACT GTAACCCCAA CCTGCTGAAC GGCCAGCTGA GCTCTCCTTT ACACTTCGCA 
GCTATCGGCG GTCATGCAGA AATCGTGCAG CTCCTCCTGC AGCACCCAGA GATCGACAGA 
CACATCGAGG ACCAGCAGAA ACGATCGCCG CTTCAAGTAT GTGAAGAAAA CAAGCAGAAC 
AACTGGGAGG AAACCGTGAA TCTCCTTCAA CAAGCCAGCA ATAAACCATA CGAGAAGGTG 
AGGATTTACC GTATGGATGG ATCGTACCGC TCTGTGGAGC TGAAGCATGG GAACAACACC 
ACCGTGCAGC AGATCATGGA GGGCATGAGA TTATCACAGG AGACCCAGCA GTATTTTACC 
ATCTGGATCT GCTCCGAAAA CCTTAGTCTG CAGCTGAAGC CGTACCACAA GCCCTTGCAG 
CACTTACGGA TGTGGAGCGA GATCGTGACG GATCTCACTG CTCTGGATCC ACAGAGAGAA 
AGTCCACAGC TCTTCCTGCG CAGAGACGTT CGGCTGCCTT TAGAGGTCGA GAAAAAGGTT 
GAAGATCCGC TGTCCATCCT CATCCTATTT GATGAAGCTC GTCACTGCCT CCTTAAAGGT 
TTCCTCTCGA CGTCAGATAA CAAGCTAATC ACGCTCGCAA GCCTCCTCCT GCAGATCATC 
TACGGAAACT ACGACAGCAA GAAGCACAAA CAGGGCTTTC TAAATGAGGA AAACCTGAAA 
TCCATAGTTC CCATTTCAAA GGTCAAGAGC AAAGCACATC ACTGGACTAA CAGGATACTT 
CACGAGTACA AGAGTCTGAG CACTAGTGAG GGTGTGAGTA AAGAAATGCA TCACCTCCAG 
AGGCTCTTCC TGCAGAACTG CTGGGATATT CCTACTTATG GTGCAGCGTT TTTCACAGGA 
CAGGTCTTCA CTAAAGCCAG CTCCAGCACA CACAAGGTGA TCCGCGTGTA TGTGGGCGTC 
AACACCAAAG GCCTGCATCT GATGAACATG GAGACAAAAG TGCTTCACCT CAGTCTGGAG 
TATGGGACAT TTATGTGGCA GCTGGGTCAG GCTGATCAAT ACGTGCAAAT CCACAGTCTG 
GAGAACAAGA AGAACTTTGT CGTTCACACC AAACAGGCTG GTCTTATCGT GAAGCTGCTG 
ATGAAGCTGA GTGGACAAAT TGCACCAAAC GACAGAGCTG TGTCTGATAA ATATGCGTAT 
GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303930.1
CDS	93..2318
Translation	MMGNQELEEVFVAVIRPKNTTSLNSKEYRAKSYEILLIEVPLEGKEKKRKKVLLGTKIHADSDRTKSILEFVDETTKPISNNQGIIGKRVVHMRKFLLDGDSGGKEASLFIVPINVKDNSKSVHHPGSPSFYCLQDIMRVCSETSAHFSSSTSKMLLALDKWLAEQHTVPHAIPALFRPAPVDRVKTNVSNPAYAVEKQTDGTLHMGYTALEIKSKLMSLEKADLCIQNPLYGSDLQYTNRVDKVIINPYFGLGAPDYSKIQIPKRDKWQHSMTSVTEDKERQWVDDFPLHRSACEGDTELLSKLLDGGFSVKQLDSDHWAPIHYACWHGKVEATKLLLEKGNCNPNLLNGQLSSPLHFAAIGGHAEIVQLLLQHPEIDRHIEDQQKRSPLQVCEENKQNNWEETVNLLQQASNKPYEKVRIYRMDGSYRSVELKHGNNTTVQQIMEGMRLSQETQQYFTIWICSENLSLQLKPYHKPLQHLRMWSEIVTDLTALDPQRESPQLFLRRDVRLPLEVEKKVEDPLSILILFDEARHCLLKGFLSTSDNKLITLASLLLQIIYGNYDSKKHKQGFLNEENLKSIVPISKVKSKAHHWTNRILHEYKSLSTSEGVSKEMHHLQRLFLQNCWDIPTYGAAFFTGQVFTKASSSTHKVIRVYVGVNTKGLHLMNMETKVLHLSLEYGTFMWQLGQADQYVQIHSLENKKNFVVHTKQAGLIVKLLMKLSGQIAPNDRAVSDKYAYG

Target ORF information:

RefSeq Version	NM_001317001.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio KRIT1 ankyrin repeat containing (krit1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317001.1

ORF Insert Sequence:

ATGATGGGAA ACCAAGAGCT AGAGGAGGTG TTTGTTGCTG TTATCCGACC AAAAAACACA 
ACCAGCCTCA ACTCCAAAGA ATACCGTGCT AAATCCTATG AGATTTTACT TATCGAAGTT 
CCCCTGGAGG GAAAAGAGAA AAAGCGGAAG AAAGTGCTGC TTGGGACTAA AATCCACGCC 
GACAGTGACC GGACAAAGTC AATTCTGGAG TTTGTGGATG AAACCACCAA GCCCATATCC 
AATAACCAGG GCATAATAGG GAAGCGTGTC GTGCACATGA GGAAGTTCCT GTTAGATGGA 
GACAGTGGAG GAAAAGAGGC CTCGTTATTT ATTGTGCCAA TTAATGTCAA AGACAACAGC 
AAGTCCGTGC ATCACCCTGG GAGCCCCAGT TTCTACTGCC TTCAGGACAT CATGCGTGTG 
TGTAGTGAAA CCAGTGCTCA TTTCTCCTCC AGCACCTCAA AGATGCTGCT CGCTCTCGAC 
AAGTGGTTAG CGGAGCAGCA CACTGTGCCT CACGCTATTC CTGCTCTGTT CCGGCCGGCT 
CCGGTGGACC GTGTGAAAAC TAACGTGAGT AACCCGGCGT ACGCAGTGGA GAAGCAGACA 
GACGGGACGC TGCACATGGG CTACACGGCT CTGGAGATCA AGAGTAAACT GATGTCTCTG 
GAGAAAGCAG ATCTGTGCAT CCAGAACCCG CTGTACGGCT CAGATCTGCA GTACACCAAC 
AGAGTGGACA AAGTCATCAT CAACCCTTAC TTTGGCTTGG GAGCTCCAGA TTACTCCAAA 
ATCCAGATTC CAAAGAGAGA CAAATGGCAG CACAGCATGA CCAGCGTCAC CGAAGACAAA 
GAGCGCCAGT GGGTGGATGA TTTCCCTCTG CACCGAAGCG CCTGTGAGGG AGACACTGAG 
CTTTTGTCTA AATTGCTGGA TGGCGGATTC TCAGTCAAAC AGCTGGACAG CGACCATTGG 
GCACCCATTC ATTATGCATG CTGGCATGGG AAGGTGGAGG CCACTAAACT GCTGCTGGAG 
AAAGGCAACT GTAACCCCAA CCTGCTGAAC GGCCAGCTGA GCTCTCCTTT ACACTTCGCA 
GCTATCGGCG GTCATGCAGA AATCGTGCAG CTCCTCCTGC AGCACCCAGA GATCGACAGA 
CACATCGAGG ACCAGCAGAA ACGATCGCCG CTTCAAGTAT GTGAAGAAAA CAAGCAGAAC 
AACTGGGAGG AAACCGTGAA TCTCCTTCAA CAAGCCAGCA ATAAACCATA CGAGAAGGTG 
AGGATTTACC GTATGGATGG ATCGTACCGC TCTGTGGAGC TGAAGCATGG GAACAACACC 
ACCGTGCAGC AGATCATGGA GGGCATGAGA TTATCACAGG AGACCCAGCA GTATTTTACC 
ATCTGGATCT GCTCCGAAAA CCTTAGTCTG CAGCTGAAGC CGTACCACAA GCCCTTGCAG 
CACTTACGGA TGTGGAGCGA GATCGTGACG GATCTCACTG CTCTGGATCC ACAGAGAGAA 
AGTCCACAGC TCTTCCTGCG CAGAGACGTT CGGCTGCCTT TAGAGGTCGA GAAAAAGGTT 
GAAGATCCGC TGTCCATCCT CATCCTATTT GATGAAGCTC GTCACTGCCT CCTTAAAGGT 
TTCCTCTCGA CGTCAGATAA CAAGCTAATC ACGCTCGCAA GCCTCCTCCT GCAGATCATC 
TACGGAAACT ACGACAGCAA GAAGCACAAA CAGGGCTTTC TAAATGAGGA AAACCTGAAA 
TCCATAGTTC CCATTTCAAA GGTCAAGAGC AAAGCACATC ACTGGACTAA CAGGATACTT 
CACGAGTACA AGAGTCTGAG CACTAGTGAG GGTGTGAGTA AAGAAATGCA TCACCTCCAG 
AGGCTCTTCC TGCAGAACTG CTGGGATATT CCTACTTATG GTGCAGCGTT TTTCACAGGA 
CAGGTCTTCA CTAAAGCCAG CTCCAGCACA CACAAGGTGA TCCGCGTGTA TGTGGGCGTC 
AACACCAAAG GCCTGCATCT GATGAACATG GAGACAAAAG TGCTTCACCT CAGTCTGGAG 
TATGGGACAT TTATGTGGCA GCTGGGTCAG GCTGATCAAT ACGTGCAAAT CCACAGTCTG 
GAGAACAAGA AGAACTTTGT CGTTCACACC AAACAGGCTG GTCTTATCGT GAAGCTGCTG 
ATGAAGCTGA GTGGACAAAT TGCACCAAAC GACAGAGCTG TGTCTGATAA ATATGCGTAT 
GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The CCM1-CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity.
Journal of cell science131(15)(2018 08)
Lisowska J,Rödel CJ,Manet S,Miroshnikova YA,Boyault C,Planus E,De Mets R,Lee HH,Destaing O,Mertani H,Boulday G,Tournier-Lasserve E,Balland M,Abdelilah-Seyfried S,Albiges-Rizo C,Faurobert E

Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis.
eLife7(2018 02)
Donat S,Lourenço M,Paolini A,Otten C,Renz M,Abdelilah-Seyfried S

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Scientific reports621540(2016 Feb)
Gistelinck C,Gioia R,Gagliardi A,Tonelli F,Marchese L,Bianchi L,Landi C,Bini L,Huysseune A,Witten PE,Staes A,Gevaert K,De Rocker N,Menten B,Malfait F,Leikin S,Carra S,Tenni R,Rossi A,De Paepe A,Coucke P,Willaert A,Forlino A

The cerebral cavernous malformation proteins CCM2L and CCM2 prevent the activation of the MAP kinase MEKK3.
Proceedings of the National Academy of Sciences of the United States of America112(46)14284-9(2015 Nov)
Cullere X,Plovie E,Bennett PM,MacRae CA,Mayadas TN

Lack of CCM1 induces hypersprouting and impairs response to flow.
Human molecular genetics23(23)6223-34(2014 Dec)
Mleynek TM,Chan AC,Redd M,Gibson CC,Davis CT,Shi DS,Chen T,Carter KL,Ling J,Blanco R,Gerhardt H,Whitehead K,Li DY

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krit1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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