ephb4b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ephb4b
Entrez Gene ID	30691
Full Name	eph receptor B4b
Synonyms	EphB-rtk8,cb417,rtk8
General protein information	Preferred Names eph receptor B4b Names eph receptor B4b eph-like receptor tyrosine kinase 8 unp164
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131417.1	NP_571492.1	ephrin type-B receptor 4b precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	EPHB4	NP_004435.3
Canis lupus familiaris (dog)	EPHB4	XP_546948.3
Mus musculus (house mouse)	Ephb4	NP_001153043.1
Rattus norvegicus (Norway rat)	Ephb4	XP_003751205.1
Macaca mulatta (Rhesus monkey)	EPHB4	XP_002803271.1
Bos taurus (cattle)	EPHB4	NP_001193197.1
Danio rerio (zebrafish)	ephb4b	NP_571492.1
Pan troglodytes (chimpanzee)	EPHB4	XP_519269.3
Danio rerio (zebrafish)	ephb4a	NP_571489.1

Gene Ontology
Bibliography

Related articles in PubMed

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Xue Y, Liu D, Cui G, Ding Y, Ai D, Gao S, Zhang Y, Suo S, Wang X, Lv P, Zhou C, Li Y, Chen X, Peng G, Jing N, Han JJ, Liu F
Cell reports27(5)1567-1578.e5(2019 04)

Pathogenic variant in EPHB4 results in central conducting lymphatic anomaly.
Li D, Wenger TL, Seiler C, March ME, Gutierrez-Uzquiza A, Kao C, Bhoj E, Tian L, Rosenbach M, Liu Y, Robinson N, Behr M, Chiavacci R, Hou C, Wang T, Bakay M, Pellegrino da Silva R, Perkins JA, Sleiman P, Levine MA, Hicks PJ, Itkin M, Dori Y, Hakonarson H
Human molecular genetics27(18)3233-3245(2018 09)

HHEX is a transcriptional regulator of the VEGFC/FLT4/PROX1 signaling axis during vascular development.
Gauvrit S, Villasenor A, Strilic B, Kitchen P, Collins MM, Marín-Juez R, Guenther S, Maischein HM, Fukuda N, Canham MA, Brickman JM, Bogue CW, Jayaraman PS, Stainier DYR
Nature communications9(1)2704(2018 07)

Genome editing of factor X in zebrafish reveals unexpected tolerance of severe defects in the common pathway.
Hu Z, Liu Y, Huarng MC, Menegatti M, Reyon D, Rost MS, Norris ZG, Richter CE, Stapleton AN, Chi NC, Peyvandi F, Joung JK, Shavit JA
Blood130(5)666-676(2017 08)

Characterisation of five novel zebrafish Eph-related receptor tyrosine kinases suggests roles in patterning the neural plate.
Cooke JE, Xu Q, Wilson SW, Holder N
Development genes and evolution206(8)515-531(1997 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ephb4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ephb4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30555	NM_131417.1 Latest version!	Danio rerio eph receptor B4b (ephb4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ephb4b ( NM_131417.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571492.1 Danio rerio eph receptor B4b (ephb4b), mRNA.

Price & Availability↑

CloneID	ODa30555
Clone ID Related Accession (Same CDS sequence)	NM_131417.1
Accession Version	NM_131417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Danio rerio(zebrafish)
Product	ephrin type-B receptor 4b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ005029.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ005029.1, BC163275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGATCGTG TTTGCTGGAT TATGGCTCTT TCCTGGTTCT GGATGGTGTC GACTGGACTC 
GTCTCGGCTG AGGAAGAGGT CCTGATGAAC ACCAAGCTGG AAACATCAGA CCTGAGATGG 
ACCATCTACC CCAGCGGTGA CCCCGAGTGG GAGGAGATGA GCGGTCTGGA TGAAGAGGGC 
AACAGCGTCC GCACCTTCCA GGTTTGCCCG ATGGACAGTT CTGTGAGCCA CTGGCTGCGC 
ACACGCTTTA TCCCCCGCCA CGGCGCGTCT CAGGTGTACG TGGAGATCCG CTTCACCATG 
ATGGAATGTT CCGCCATGCC GGCCAGCTTC CGCACCTGCA AGGAGACCTT TAACCTTTAC 
TACTATCAGA GCGACGAGGA CACGGCCAGC GCCACACACC CCGCCTGGAT GGAGAACCCC 
TACAGCAAGG TGGACACAGT TGCTGCTGAC TTCCTGTTGC GTCGCGGCGG AGAGAGGAAG 
TCCAATGTGA AGACGGTGCG TGTGGGTCCG CTGTCGTTGT TTGGCTTCTA CCTGGCGTTT 
CAGACTCAGG GCGCGTGCAT GGCTCTGCTG TCCGTCAGGG TTTTCTTCAA GAAGTGTCCT 
GCCGTCTCCC GCGCCTTCTC CTCCTTCCCG GAGACGCTCC CTCACTCGCT GGTGCAGCAG 
GCGGAGGGTG TGTGCGTGGA TAACTCCGCC CCCACTGGAC AGTGCACAGC TCCACCCACT 
ATGTTCTGCG GGGAGGACGG TCAGTGGGTG GGGCCTCCGT CGTCTACATG TGCTTGTAAA 
CCAGGATACG AACCTGTCGA CTCCGACCGC TGCAGGGCGT GTGGATTGGG CCAGTATAAA 
GCATCTGTCG GAGGGAGCTT GTGTCGTGTG TGTCCAGACA ACAGTAACAC ACACTCCGCT 
GGATCCAGTC TGTGTGTGTG TCGTCCAGGA TACCACCGAG CAACCAGTGA CCTTCCGGAC 
TCAGCCTGCA CCAAACCGCC ATCGGCTCCG CGTAGCATCA TCTATCAGAT CAATGATACG 
GTGGTCACCC TGGAGTGGTC CGAGCCGCTA GATCGCGGTG GACGTTCTGA TCTCTCCTAC 
AGTGTGGAGT GTATGCACTG CAGAGGCTCC CTATGCGTCC AGTGTGCAGA CAGCATCACC 
TATAGGCCGG GTCAGATGGG CGTGTCCGGG AGGCGTGTCA TCATCCGTGG ACTCCTCCCA 
CACACCACCT ACACCTTCAC TGTGCTGGCC CAGAACGGCG TCAGCGCTGT CAGTCACACA 
AGCCCCGCCT CCAGCTCCGT CAACATCACT ACCAGCAGAG ATGTGGCTGT CCCTGTGTCT 
GGTATCAGGA GGATTAAAGC ATCTGAGAGC AGCGTGTCCA TCAGCTGGAC GGTTCCTCCA 
CAAACGCAGC ACAGCATTCA GGATTATCAG CTGCGCTACA GTCTGAAGGG TCAGGATGAT 
GGATGGCAGT ACGTCAGCAG CAGGAGCAGC TCAGTGGTTC TGAATGATCT GAGTCGAGCG 
TCGCAGTATC AGGTGCAGGT CAGAGCCAGA ACCGCTGCGG GATACGGACA CTTCAGCAGC 
GCCGTCAGCA TCAGCACGCT ACCTGATGAT GAAGAGTCTC CGTCGCGGCT GATGCTCACT 
GGTGTCCTGG TTGCTATAGG ACTGCTCATC CTCATCGCCG TGGTGATTGT TGCCGTGTTC 
TGCTTCAGAC GCTCGACTCG GAGGAGGGAT CCAGACCCGG ATAAGAGCGG CCAGTTCCTG 
ATGGGTCAGG GGATTAAAGT GTACATTGAT CCGTTCACGT ACGAGGACCC GAATGAGGCT 
GTGCGGGAAT TCGCTAAAGA GATCGACGTG TCGTTTGTGA AGATCGAGGA GGTCATCGGT 
GCCGGTGAGT TTGGTGAGGT GTGTCGCGGC CGGCTCAAGG TTCCCGGGAA GAAAGAGAAC 
TACGTGGCCA TCAAGACGCT GAAGGGCGGA TACACGGACA AGCAGAGGCG TGACTTCCTG 
TCTGAGGCGT CAATCATGGG TCAGTTCCAG CACCCCAACA TCATTCATCT GGAGGGAGTG 
ATCACAGCTT CCTGTCCCGT CATGATTCTG ACCGAGTACA TGGAGAACGG AGCACTCGAC 
TCCTTCCTGC GGCTGAATGA TGGTCAGTTC ACTCCTATCC AGCTGGTGGG GATGCTGCGT 
GGAATCGCGT CTGGAATGAA GTATCTGTCT GAGATGAGCT TCGTTCATCG TGATCTGGCG 
GCGAGGAACA TCCTGGTCAA CAGTAACCTG GTGTGCAAAG TGTCCGACTT CGGCCTGTCC 
CGATTCCTGA CGGAGAACTC GTCTGATCCC ACCTACACCA GCTCACTGGG CGGGAAGATC 
CCGATCCGCT GGACGGCTCC TGAAGCCATC GCCTTCCGCA AGTTCACCTC AGCGTCAGAC 
GTCTGGAGCT ACGGCATCGT CATGTGGGAG GTGATGTCAT TCGGAGAGAG GCCGTACTGG 
GACATGAGCA ACCAGGACGT GATAAACGCC ATCGAGCAGG ACTACCGCCT GCCGCCTCCC 
CCAGAATGCC CTGCGTCTCT CCATCAGCTG ATGCTGGACT GCTGGCAGAA GGAGAGATCG 
TCCCGGCCGC GCTTCTGCGC CATCGTCTCT GCCCTCGACA GACTGATCCG CAACCCTGCC 
TCACTCAAGA TCACCGGACG CATCCCTGAT GGGCCGTCTC ACCCTCTCCT GGACCAGCGT 
GCTCCTCCTC CGCTCTCTCA CTGCAGCTCA GTTGCTGATT GGCTGAGAGC CATAAAGATG 
GAGCGATACG AGGACGCGTT CATGCAGGCC GGATTCACAG CCATCCAACA CATCACACAC 
ATCTCCACTG AGGATCTTCT GCGGATCGGC GTGACTTTAG CGGGACACCA GAAGAAGATT 
CTGTCCAGCG TCCAAACACT GCGCATCCAC GGCGGATCCC TGCGATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571492.1
CDS	1..2931
Translation	MDRVCWIMALSWFWMVSTGLVSAEEEVLMNTKLETSDLRWTIYPSGDPEWEEMSGLDEEGNSVRTFQVCPMDSSVSHWLRTRFIPRHGASQVYVEIRFTMMECSAMPASFRTCKETFNLYYYQSDEDTASATHPAWMENPYSKVDTVAADFLLRRGGERKSNVKTVRVGPLSLFGFYLAFQTQGACMALLSVRVFFKKCPAVSRAFSSFPETLPHSLVQQAEGVCVDNSAPTGQCTAPPTMFCGEDGQWVGPPSSTCACKPGYEPVDSDRCRACGLGQYKASVGGSLCRVCPDNSNTHSAGSSLCVCRPGYHRATSDLPDSACTKPPSAPRSIIYQINDTVVTLEWSEPLDRGGRSDLSYSVECMHCRGSLCVQCADSITYRPGQMGVSGRRVIIRGLLPHTTYTFTVLAQNGVSAVSHTSPASSSVNITTSRDVAVPVSGIRRIKASESSVSISWTVPPQTQHSIQDYQLRYSLKGQDDGWQYVSSRSSSVVLNDLSRASQYQVQVRARTAAGYGHFSSAVSISTLPDDEESPSRLMLTGVLVAIGLLILIAVVIVAVFCFRRSTRRRDPDPDKSGQFLMGQGIKVYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVCRGRLKVPGKKENYVAIKTLKGGYTDKQRRDFLSEASIMGQFQHPNIIHLEGVITASCPVMILTEYMENGALDSFLRLNDGQFTPIQLVGMLRGIASGMKYLSEMSFVHRDLAARNILVNSNLVCKVSDFGLSRFLTENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPECPASLHQLMLDCWQKERSSRPRFCAIVSALDRLIRNPASLKITGRIPDGPSHPLLDQRAPPPLSHCSSVADWLRAIKMERYEDAFMQAGFTAIQHITHISTEDLLRIGVTLAGHQKKILSSVQTLRIHGGSLRY

Target ORF information:

RefSeq Version	NM_131417.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eph receptor B4b (ephb4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131417.1

ORF Insert Sequence:

ATGGATCGTG TTTGCTGGAT TATGGCTCTT TCCTGGTTCT GGATGGTGTC GACTGGACTC 
GTCTCGGCTG AGGAAGAGGT CCTGATGAAC ACCAAGCTGG AAACATCAGA CCTGAGATGG 
ACCATCTACC CCAGCGGTGA CCCCGAGTGG GAGGAGATGA GCGGTCTGGA TGAAGAGGGC 
AACAGCGTCC GCACCTTCCA GGTTTGCCCG ATGGACAGTT CTGTGAGCCA CTGGCTGCGC 
ACACGCTTTA TCCCCCGCCA CGGCGCGTCT CAGGTGTACG TGGAGATCCG CTTCACCATG 
ATGGAATGTT CCGCCATGCC GGCCAGCTTC CGCACCTGCA AGGAGACCTT TAACCTTTAC 
TACTATCAGA GCGACGAGGA CACGGCCAGC GCCACACACC CCGCCTGGAT GGAGAACCCC 
TACAGCAAGG TGGACACAGT TGCTGCTGAC TTCCTGTTGC GTCGCGGCGG AGAGAGGAAG 
TCCAATGTGA AGACGGTGCG TGTGGGTCCG CTGTCGTTGT TTGGCTTCTA CCTGGCGTTT 
CAGACTCAGG GCGCGTGCAT GGCTCTGCTG TCCGTCAGGG TTTTCTTCAA GAAGTGTCCT 
GCCGTCTCCC GCGCCTTCTC CTCCTTCCCG GAGACGCTCC CTCACTCGCT GGTGCAGCAG 
GCGGAGGGTG TGTGCGTGGA TAACTCCGCC CCCACTGGAC AGTGCACAGC TCCACCCACT 
ATGTTCTGCG GGGAGGACGG TCAGTGGGTG GGGCCTCCGT CGTCTACATG TGCTTGTAAA 
CCAGGATACG AACCTGTCGA CTCCGACCGC TGCAGGGCGT GTGGATTGGG CCAGTATAAA 
GCATCTGTCG GAGGGAGCTT GTGTCGTGTG TGTCCAGACA ACAGTAACAC ACACTCCGCT 
GGATCCAGTC TGTGTGTGTG TCGTCCAGGA TACCACCGAG CAACCAGTGA CCTTCCGGAC 
TCAGCCTGCA CCAAACCGCC ATCGGCTCCG CGTAGCATCA TCTATCAGAT CAATGATACG 
GTGGTCACCC TGGAGTGGTC CGAGCCGCTA GATCGCGGTG GACGTTCTGA TCTCTCCTAC 
AGTGTGGAGT GTATGCACTG CAGAGGCTCC CTATGCGTCC AGTGTGCAGA CAGCATCACC 
TATAGGCCGG GTCAGATGGG CGTGTCCGGG AGGCGTGTCA TCATCCGTGG ACTCCTCCCA 
CACACCACCT ACACCTTCAC TGTGCTGGCC CAGAACGGCG TCAGCGCTGT CAGTCACACA 
AGCCCCGCCT CCAGCTCCGT CAACATCACT ACCAGCAGAG ATGTGGCTGT CCCTGTGTCT 
GGTATCAGGA GGATTAAAGC ATCTGAGAGC AGCGTGTCCA TCAGCTGGAC GGTTCCTCCA 
CAAACGCAGC ACAGCATTCA GGATTATCAG CTGCGCTACA GTCTGAAGGG TCAGGATGAT 
GGATGGCAGT ACGTCAGCAG CAGGAGCAGC TCAGTGGTTC TGAATGATCT GAGTCGAGCG 
TCGCAGTATC AGGTGCAGGT CAGAGCCAGA ACCGCTGCGG GATACGGACA CTTCAGCAGC 
GCCGTCAGCA TCAGCACGCT ACCTGATGAT GAAGAGTCTC CGTCGCGGCT GATGCTCACT 
GGTGTCCTGG TTGCTATAGG ACTGCTCATC CTCATCGCCG TGGTGATTGT TGCCGTGTTC 
TGCTTCAGAC GCTCGACTCG GAGGAGGGAT CCAGACCCGG ATAAGAGCGG CCAGTTCCTG 
ATGGGTCAGG GGATTAAAGT GTACATTGAT CCGTTCACGT ACGAGGACCC GAATGAGGCT 
GTGCGGGAAT TCGCTAAAGA GATCGACGTG TCGTTTGTGA AGATCGAGGA GGTCATCGGT 
GCCGGTGAGT TTGGTGAGGT GTGTCGCGGC CGGCTCAAGG TTCCCGGGAA GAAAGAGAAC 
TACGTGGCCA TCAAGACGCT GAAGGGCGGA TACACGGACA AGCAGAGGCG TGACTTCCTG 
TCTGAGGCGT CAATCATGGG TCAGTTCCAG CACCCCAACA TCATTCATCT GGAGGGAGTG 
ATCACAGCTT CCTGTCCCGT CATGATTCTG ACCGAGTACA TGGAGAACGG AGCACTCGAC 
TCCTTCCTGC GGCTGAATGA TGGTCAGTTC ACTCCTATCC AGCTGGTGGG GATGCTGCGT 
GGAATCGCGT CTGGAATGAA GTATCTGTCT GAGATGAGCT TCGTTCATCG TGATCTGGCG 
GCGAGGAACA TCCTGGTCAA CAGTAACCTG GTGTGCAAAG TGTCCGACTT CGGCCTGTCC 
CGATTCCTGA CGGAGAACTC GTCTGATCCC ACCTACACCA GCTCACTGGG CGGGAAGATC 
CCGATCCGCT GGACGGCTCC TGAAGCCATC GCCTTCCGCA AGTTCACCTC AGCGTCAGAC 
GTCTGGAGCT ACGGCATCGT CATGTGGGAG GTGATGTCAT TCGGAGAGAG GCCGTACTGG 
GACATGAGCA ACCAGGACGT GATAAACGCC ATCGAGCAGG ACTACCGCCT GCCGCCTCCC 
CCAGAATGCC CTGCGTCTCT CCATCAGCTG ATGCTGGACT GCTGGCAGAA GGAGAGATCG 
TCCCGGCCGC GCTTCTGCGC CATCGTCTCT GCCCTCGACA GACTGATCCG CAACCCTGCC 
TCACTCAAGA TCACCGGACG CATCCCTGAT GGGCCGTCTC ACCCTCTCCT GGACCAGCGT 
GCTCCTCCTC CGCTCTCTCA CTGCAGCTCA GTTGCTGATT GGCTGAGAGC CATAAAGATG 
GAGCGATACG AGGACGCGTT CATGCAGGCC GGATTCACAG CCATCCAACA CATCACACAC 
ATCTCCACTG AGGATCTTCT GCGGATCGGC GTGACTTTAG CGGGACACCA GAAGAAGATT 
CTGTCCAGCG TCCAAACACT GCGCATCCAC GGCGGATCCC TGCGATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Cell reports27(5)1567-1578.e5(2019 04)
Xue Y,Liu D,Cui G,Ding Y,Ai D,Gao S,Zhang Y,Suo S,Wang X,Lv P,Zhou C,Li Y,Chen X,Peng G,Jing N,Han JJ,Liu F

Pathogenic variant in EPHB4 results in central conducting lymphatic anomaly.
Human molecular genetics27(18)3233-3245(2018 09)
Li D,Wenger TL,Seiler C,March ME,Gutierrez-Uzquiza A,Kao C,Bhoj E,Tian L,Rosenbach M,Liu Y,Robinson N,Behr M,Chiavacci R,Hou C,Wang T,Bakay M,Pellegrino da Silva R,Perkins JA,Sleiman P,Levine MA,Hicks PJ,Itkin M,Dori Y,Hakonarson H

HHEX is a transcriptional regulator of the VEGFC/FLT4/PROX1 signaling axis during vascular development.
Nature communications9(1)2704(2018 07)
Gauvrit S,Villasenor A,Strilic B,Kitchen P,Collins MM,Marín-Juez R,Guenther S,Maischein HM,Fukuda N,Canham MA,Brickman JM,Bogue CW,Jayaraman PS,Stainier DYR

Genome editing of factor X in zebrafish reveals unexpected tolerance of severe defects in the common pathway.
Blood130(5)666-676(2017 08)
Hu Z,Liu Y,Huarng MC,Menegatti M,Reyon D,Rost MS,Norris ZG,Richter CE,Stapleton AN,Chi NC,Peyvandi F,Joung JK,Shavit JA

Characterisation of five novel zebrafish Eph-related receptor tyrosine kinases suggests roles in patterning the neural plate.
Development genes and evolution206(8)515-531(1997 May)
Cooke JE,Xu Q,Wilson SW,Holder N

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ephb4b cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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