wnt8b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wnt8b
Entrez Gene ID	30144
Full Name	wingless-type MMTV integration site family, member 8b
Synonyms	wnt-8b
General protein information	Preferred Names wingless-type MMTV integration site family, member 8b Names protein Wnt-8b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130959.2	NP_571034.1	protein Wnt-8b precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Wnt8b	NP_001099829.1
Gallus gallus (chicken)	WNT8B	XP_426508.3
Homo sapiens (human)	WNT8B	NP_003384.2
Canis lupus familiaris (dog)	WNT8B	XP_005637677.1
Pan troglodytes (chimpanzee)	WNT8B	XP_521584.2
Macaca mulatta (Rhesus monkey)	WNT8B	XP_001108075.1
Mus musculus (house mouse)	Wnt8b	NP_035850.2
Danio rerio (zebrafish)	wnt8b	NP_571034.1
Xenopus tropicalis (tropical clawed frog)	wnt8b	XP_004915998.1
Bos taurus (cattle)	WNT8B	XP_002698537.2

Gene Ontology
Bibliography

Related articles in PubMed

Wnt9a Is Required for the Aortic Amplification of Nascent Hematopoietic Stem Cells.
Grainger S, Richter J, Palazón RE, Pouget C, Lonquich B, Wirth S, Grassme KS, Herzog W, Swift MR, Weinstein BM, Traver D, Willert K
Cell reports17(6)1595-1606(2016 11)

scaRNAs regulate splicing and vertebrate heart development.
Patil P, Kibiryeva N, Uechi T, Marshall J, O'Brien JE Jr, Artman M, Kenmochi N, Bittel DC
Biochimica et biophysica acta1852(8)1619-29(2015 Aug)

Identification of Wnt Genes Expressed in Neural Progenitor Zones during Zebrafish Brain Development.
Duncan RN, Panahi S, Piotrowski T, Dorsky RI
PloS one10(12)e0145810(2015)

Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway.
Lu FI, Sun YH, Wei CY, Thisse C, Thisse B
Nature communications55368(2014 Nov)

Retinal injury, growth factors, and cytokines converge on β-catenin and pStat3 signaling to stimulate retina regeneration.
Wan J, Zhao XF, Vojtek A, Goldman D
Cell reports9(1)285-297(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following wnt8b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnt8b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02093	NM_130959.2 Latest version!	Danio rerio wingless-type MMTV integration site family, member 8b (wnt8b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wnt8b ( NM_130959.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571034.1 Danio rerio wingless-type MMTV integration site family, member 8b (wnt8b), mRNA.

Price & Availability↑

CloneID	ODa02093
Clone ID Related Accession (Same CDS sequence)	NM_130959.2
Accession Version	NM_130959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550160000000
Organism	Danio rerio(zebrafish)
Product	protein Wnt-8b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U10870.1. On Aug 31, 2012 this sequence version replaced NM_130959.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U10870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476777, SAMEA4476800 [ECO:0000348] ##Evidence-Data-END##

ATGTTCATGC ATTTGGAGGT TTATTATTAC GCTTTCATCT TGATGGCTCA CATGAAGACT 
TGCTGCGGTT GGTCAGTGAA TAATTTCCTG ATGACCGGCC CAAAGGCCTA CTTGATCTAC 
TCCAGCAGTG TAGCCGCAGG AGCTCAGAGT GGGATTGAAG AGTGCAAGTA TCAGTTTGCG 
TGGGACCGCT GGAAGTGCCC GGAGAGGGCC CTTCAGCTCT CCACCCACAG CGGACTCAGA 
AGCGCAAACA GAGAGACAGC GTTTTTCCAT GCCATTAGTT CTGCTGGAGT CATGTATACC 
CTCACCAGGA ACTGTAGCCT TGGTGACTTT GATAACTGTG GATGTGATGA TACCCGAAAC 
GGCCAGCGAG GTGGTCAAGG GTGGCTATGG GGAGGCTGCA GTGATAACGT GGGATTTGGA 
GAGGTGATCT CCAAACAGTT TGTGGATGCG CTGGAAACCG GACAGGATGC ACGAGCCGCC 
ATGAACCTGC ACAACAACGA AGTAGGACGC AAGGCAGTGA AAGGAACCAT GCAGAGGACA 
TGCAAGTGCC ATGGAGTGTC TGGCAGCTGC ACCACTCAGA CCTGCTGGCT ACAGTTGCCC 
GAGTTTCGAG AAGTGGGAAA CTACCTAAAA GAGAAGTACC ACCGGGCGGT GAAGGTGGAT 
CTGCTGCGCG GTGCAGGGAA CAGCGCTGCC AGCCGTGGAG CCATTGCTGA AACCTTCAAC 
TCCATTTCAC GGAAAGAGCT GGTGCATTTG GAGGATTCTC CAGACTACTG CTTGGAGAAC 
CGCACTCTAG GCTTGCCAGG TACTGAAGGG CGCGAGTGTT TGAGGAAGGG CAAGAATCTG 
AGTAAATGGG AGAAGCGCAC GTGTAAGCGG CTGTGTGGAG ACTGCGGTTT GGCTGTGGAG 
GAGCGCAGGG CCGAAACCGT GTCTAGCTGT AACTGCAAGT TCCACTGGTG TTGTGCTGTG 
AAGTGCGAGC AGTGCCGCAA AACTGTCACA AAGTACTACT GTGTGAAGAG AAGCAAACGG 
GTCAAGAATG ACAATGCCAG CCGGAGGAAA AGCTATCGGT TGAAGAAGAA GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571034.1
CDS	30..1106
Translation	MFMHLEVYYYAFILMAHMKTCCGWSVNNFLMTGPKAYLIYSSSVAAGAQSGIEECKYQFAWDRWKCPERALQLSTHSGLRSANRETAFFHAISSAGVMYTLTRNCSLGDFDNCGCDDTRNGQRGGQGWLWGGCSDNVGFGEVISKQFVDALETGQDARAAMNLHNNEVGRKAVKGTMQRTCKCHGVSGSCTTQTCWLQLPEFREVGNYLKEKYHRAVKVDLLRGAGNSAASRGAIAETFNSISRKELVHLEDSPDYCLENRTLGLPGTEGRECLRKGKNLSKWEKRTCKRLCGDCGLAVEERRAETVSSCNCKFHWCCAVKCEQCRKTVTKYYCVKRSKRVKNDNASRRKSYRLKKKH

Target ORF information:

RefSeq Version	NM_130959.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wingless-type MMTV integration site family, member 8b (wnt8b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130959.2

ORF Insert Sequence:

ATGTTCATGC ATTTGGAGGT TTATTATTAC GCTTTCATCT TGATGGCTCA CATGAAGACT 
TGCTGCGGTT GGTCAGTGAA TAATTTCCTG ATGACCGGCC CAAAGGCCTA CTTGATCTAC 
TCCAGCAGTG TAGCCGCAGG AGCTCAGAGT GGGATTGAAG AGTGCAAGTA TCAGTTTGCG 
TGGGACCGCT GGAAGTGCCC GGAGAGGGCC CTTCAGCTCT CCACCCACAG CGGACTCAGA 
AGCGCAAACA GAGAGACAGC GTTTTTCCAT GCCATTAGTT CTGCTGGAGT CATGTATACC 
CTCACCAGGA ACTGTAGCCT TGGTGACTTT GATAACTGTG GATGTGATGA TACCCGAAAC 
GGCCAGCGAG GTGGTCAAGG GTGGCTATGG GGAGGCTGCA GTGATAACGT GGGATTTGGA 
GAGGTGATCT CCAAACAGTT TGTGGATGCG CTGGAAACCG GACAGGATGC ACGAGCCGCC 
ATGAACCTGC ACAACAACGA AGTAGGACGC AAGGCAGTGA AAGGAACCAT GCAGAGGACA 
TGCAAGTGCC ATGGAGTGTC TGGCAGCTGC ACCACTCAGA CCTGCTGGCT ACAGTTGCCC 
GAGTTTCGAG AAGTGGGAAA CTACCTAAAA GAGAAGTACC ACCGGGCGGT GAAGGTGGAT 
CTGCTGCGCG GTGCAGGGAA CAGCGCTGCC AGCCGTGGAG CCATTGCTGA AACCTTCAAC 
TCCATTTCAC GGAAAGAGCT GGTGCATTTG GAGGATTCTC CAGACTACTG CTTGGAGAAC 
CGCACTCTAG GCTTGCCAGG TACTGAAGGG CGCGAGTGTT TGAGGAAGGG CAAGAATCTG 
AGTAAATGGG AGAAGCGCAC GTGTAAGCGG CTGTGTGGAG ACTGCGGTTT GGCTGTGGAG 
GAGCGCAGGG CCGAAACCGT GTCTAGCTGT AACTGCAAGT TCCACTGGTG TTGTGCTGTG 
AAGTGCGAGC AGTGCCGCAA AACTGTCACA AAGTACTACT GTGTGAAGAG AAGCAAACGG 
GTCAAGAATG ACAATGCCAG CCGGAGGAAA AGCTATCGGT TGAAGAAGAA GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Wnt9a Is Required for the Aortic Amplification of Nascent Hematopoietic Stem Cells.
Cell reports17(6)1595-1606(2016 11)
Grainger S,Richter J,Palazón RE,Pouget C,Lonquich B,Wirth S,Grassme KS,Herzog W,Swift MR,Weinstein BM,Traver D,Willert K

scaRNAs regulate splicing and vertebrate heart development.
Biochimica et biophysica acta1852(8)1619-29(2015 Aug)
Patil P,Kibiryeva N,Uechi T,Marshall J,O'Brien JE Jr,Artman M,Kenmochi N,Bittel DC

Identification of Wnt Genes Expressed in Neural Progenitor Zones during Zebrafish Brain Development.
PloS one10(12)e0145810(2015)
Duncan RN,Panahi S,Piotrowski T,Dorsky RI

Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway.
Nature communications55368(2014 Nov)
Lu FI,Sun YH,Wei CY,Thisse C,Thisse B

Retinal injury, growth factors, and cytokines converge on β-catenin and pStat3 signaling to stimulate retina regeneration.
Cell reports9(1)285-297(2014 Oct)
Wan J,Zhao XF,Vojtek A,Goldman D