ORF » Species Summary » Danio rerio » ube2kb cDNA ORF clone

ube2kb cDNA ORF clone, Danio rerio(zebrafish)

The following ube2kb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube2kb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa04270 NM_001008611.2
Latest version!		 Danio rerio ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) (ube2kb), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ube2kb ( NM_001008611.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001008611.1 Danio rerio ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) (ube2kb), mRNA.
    Price & Availability↑
    CloneID ODa04270
    Clone ID Related Accession (Same CDS sequence) NM_001008611.2
    Accession Version NM_001008611.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466784000000
    Organism Danio rerio(zebrafish)
    Product ubiquitin-conjugating enzyme E2 K
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC086816.1, DN767847.1, AL715279.1, EH554183.1 and EH575297.1. On Oct 31, 2008 this sequence version replaced NM_001008611.1. ##Evidence-Data-START## Transcript exon combination :: BC086816.1, EE324936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAAATA TCGCTGTGCA GAGGATCAAG CGAGAGTTTA AAGAAGTGCT GAAAAGCGAA 
    GAGACGAGTA AAAATCAAAT AAAAGTAGAT CTGGTGGATG AAAACTTTAC AGAGTTAAAG 
    GGAGAAATAG CAGGACCGCC AGACACACCA TATGAAGGGG GAAGATATCA ACTAGAAATC 
    AAGATCCCTG AGACGTATCC ATTTAATCCA CCCAAGGTGC GGTTTATTAC TAAGATCTGG 
    CATCCCAATA TAAGTTCTGT TACCGGGGCG ATATGTTTGG ACATTTTAAA AGACCAGTGG 
    GCTGCTGCTA TGACTCTGCG AACAGTTCTG CTCTCACTAC AGGCTCTGTT AGCTGCAGCA 
    GAGCCCGATG ATCCACAGGA CGCTGTGGTA GCCAATCAGT ATAAGCAGAA TCCAGAAATG 
    TTCAAACAAA CTGCTCGTCT CTGGTCTCAC GTTTACGCTG GTGCTCCTGT GTCCAGTCCA 
    GAGTACACAC GCAAAATAGA CAAACTTTGT GCAATGGGCT TTGACAAAAA TGCAGTAATA 
    GTAGCCTTGT CCTCAAAATC ATGGGACGTG GAGACGGCGA CAGAGCTGCT ACTAAGCAAC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001008611.1
    CDS456..1058
    Translation

    Target ORF information:

    RefSeq Version NM_001008611.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) (ube2kb), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001008611.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAAATA TCGCTGTGCA GAGGATCAAG CGAGAGTTTA AAGAAGTGCT GAAAAGCGAA 
    GAGACGAGTA AAAATCAAAT AAAAGTAGAT CTGGTGGATG AAAACTTTAC AGAGTTAAAG 
    GGAGAAATAG CAGGACCGCC AGACACACCA TATGAAGGGG GAAGATATCA ACTAGAAATC 
    AAGATCCCTG AGACGTATCC ATTTAATCCA CCCAAGGTGC GGTTTATTAC TAAGATCTGG 
    CATCCCAATA TAAGTTCTGT TACCGGGGCG ATATGTTTGG ACATTTTAAA AGACCAGTGG 
    GCTGCTGCTA TGACTCTGCG AACAGTTCTG CTCTCACTAC AGGCTCTGTT AGCTGCAGCA 
    GAGCCCGATG ATCCACAGGA CGCTGTGGTA GCCAATCAGT ATAAGCAGAA TCCAGAAATG 
    TTCAAACAAA CTGCTCGTCT CTGGTCTCAC GTTTACGCTG GTGCTCCTGT GTCCAGTCCA 
    GAGTACACAC GCAAAATAGA CAAACTTTGT GCAATGGGCT TTGACAAAAA TGCAGTAATA 
    GTAGCCTTGT CCTCAAAATC ATGGGACGTG GAGACGGCGA CAGAGCTGCT ACTAAGCAAC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Zebrafish Ciliopathy Screen Plus Human Mutational Analysis Identifies C21orf59 and CCDC65 Defects as Causing Primary Ciliary Dyskinesia.
    American journal of human genetics93(4)672-86(2013 Oct)
    Austin-Tse C,Halbritter J,Zariwala MA,Gilberti RM,Gee HY,Hellman N,Pathak N,Liu Y,Panizzi JR,Patel-King RS,Tritschler D,Bower R,O'Toole E,Porath JD,Hurd TW,Chaki M,Diaz KA,Kohl S,Lovric S,Hwang DY,Braun DA,Schueler M,Airik R,Otto EA,Leigh MW,Noone PG,Carson JL,Davis SD,Pittman JE,Ferkol TW,Atkinson JJ,Olivier KN,Sagel SD,Dell SD,Rosenfeld M,Milla CE,Loges NT,Omran H,Porter ME,King SM,Knowles MR,Drummond IA,Hildebrandt F


    Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
    Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
    Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,


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