sim1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sim1a
Entrez Gene ID	260351
Full Name	single-minded family bHLH transcription factor 1a
Synonyms	sim1
General protein information	Preferred Names single-minded family bHLH transcription factor 1a Names single-minded homolog 1-A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_178222.3	NP_835740.2	single-minded homolog 1-A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIM1	NP_001074957.1
Danio rerio (zebrafish)	sim1a	NP_835740.1
Xenopus tropicalis (tropical clawed frog)	sim1	XP_004914602.1
Homo sapiens (human)	SIM1	NP_005059.2
Canis lupus familiaris (dog)	SIM1	XP_539058.2
Mus musculus (house mouse)	Sim1	NP_035506.2
Gallus gallus (chicken)	SIM1	XP_004940413.1
Macaca mulatta (Rhesus monkey)	SIM1	XP_001086054.1
Bos taurus (cattle)	SIM1	NP_001179901.1
Rattus norvegicus (Norway rat)	Sim1	NP_001101111.1

Gene Ontology
Bibliography

Related articles in PubMed

Homeogene emx1 is required for nephron distal segment development in zebrafish.
Morales EE, Handa N, Drummond BE, Chambers JM, Marra AN, Addiego A, Wingert RA
Scientific reports8(1)18038(2018 12)

Chambers JM, Poureetezadi SJ, Addiego A, Lahne M, Wingert RA
eLife7(2018 11)

Separate roles for Med12 and Wnt signaling in regulation of oxytocin expression.
Spikol ED, Glasgow E
Biology open7(3)(2018 Mar)

Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid-Contacting KA Spinal Neurons.
Andrzejczuk LA, Banerjee S, England SJ, Voufo C, Kamara K, Lewis KE
Frontiers in neuroscience12170(2018)

Transcriptional Complexity and Distinct Expression Patterns of
Kondrychyn I, Robra L, Thirumalai V
G3 (Bethesda, Md.)7(8)2577-2593(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sim1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sim1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa60468	NM_178222.3 Latest version!	Danio rerio SIM bHLH transcription factor 1a (sim1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sim1a ( NM_178222.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_835740.2 Danio rerio SIM bHLH transcription factor 1a (sim1a), mRNA.

Price & Availability↑

CloneID	ODa60468
Clone ID Related Accession (Same CDS sequence)	NM_178222.3
Accession Version	NM_178222.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1567785600000
Organism	Danio rerio(zebrafish)
Product	single-minded homolog 1-A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU905511.9 and BX005277.11. On Sep 1, 2015 this sequence version replaced NM_178222.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY028626.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA2168448 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGAGA AGTCGAAAAA CGCGGCGCGC ACGCGGCGGG AGAAGGAAAA CAGCGAGTTT 
TACGAGCTCG CCAAACTGCT GCCTCTGCCC TCCGCCATCA CCTCACAGCT GGACAAAGCC 
TCCATCATCA GACTCACCAC CAGCTACCTG AAAATGAGGA TAGTCTTCCC TGAAGGTCTT 
GGGGAATCTT GGGGCCATGT GAGTCGAACG ACTTCACTGG AAAATGTCGG CCGAGAATTA 
GGATCACATC TGCTTCAGAC GTTAGACGGC TTCATCTTTG TGGTGGCTCC TGATGGGAAA 
ATCCTGTACA TTTCAGAAAC GGCGTCAGTG CATTTAGGCT TGTCACAGGT AGAGCTGACG 
GGGAACAGCA TTTATGAGTA CATCCACCCC GCAGACCACG ACGAAATGAC CGCAGTGCTG 
ACGGCTCACC AGCCCTATCA TTCACACTTC GTCCATGAGT ACGAAATGGA GCGCTCTTTT 
TTCCTGAGGA TGAAATGTGT CCTTGCTAAA CGTAACGCAG GATTGACGTG CGGTGGTTAC 
AAGGTCATCC ACTGCAGCGG GTACCTGAAG ATCCGTCAGT ACAGTTTAGA CATGTCTCCG 
TTCGACGGCT GCTATCAGAA TGTGGGTCTG GTGGCTGTAG GTCACTCGCT CCCGCCCAGC 
GCAGTCACCG AGATCAAACT GCACAGCAAC ATGTTCATGT TCAGAGCCAG CCTGGACATG 
AAGCTCATCT TCCTGGACTC CAGGGTTGCG GAGCTCACTG GCTACGAGCC GCAAGACTTA 
ATAGAGAAGA CACTTTATCA TCACGTCCAC AGCTGTGACA CTTTCCATCT CCGCTGCGCG 
CACCACTTGT TGCTGGTAAA AGGACAAGTC ACCACTAAAT ACTACCGATT CCTGGCGAAG 
CAGGGCGGAT GGGTGTGGGT ACAGAGTTAC GCCACCATCG TCCACAACAG CAGATCATCC 
AGACCTCACT GCATCGTCAG CGTCAATTAC GTCCTCACGG ACACGGAGTA CAAAGGACTA 
CAGTTGTCTT TGGACCAGGC GGCGTCCACC AAACCCTCCT TCACCTACAA CAGCCCCTCC 
AACCCCGTCA CCGAGAACAG AAGAGTTGGA AAAAGCAGAG TCTCCCGAAC CAAGACCAAA 
ACAAGACTCT CTCCGTACTC ACAGTATCCA GGTTTCCCCA CAGATCGCTC GGAGTCGGAT 
CAGGACAGCC CATGGGGTGG GAGTCCACTG ACTGATTCTG CATCTCCTCA GCTTCTGGAG 
CAGTGTGAAG GCATCGAGTC CTCATGCGTG TACCGGCAGT TCTCGGACCC CCGCTCGCTG 
TGCTATGGTT TACCCCTGAC GGAAGATCAC CACACCTCCA ACGAGCTCTA CAGCCACCCT 
CACTCGGAGT CCTGCGAGAG GGGCTGCTGT AAAGCCGGAC GCTACTTTCT GGGCACCCCT 
CAGCCCGGAC GAGAGGCATG GTGGGGCGCA GCCCGCTCGG TCCTCCCCTT ACCCAAATCT 
TCACCTGAGA ATGGGGACAG TTTTGAAGGC GTGATGCCTC ACATCGCTTC TATCCATAGC 
CTGCAAGTGA GGGGTCACTG GGATGAGGAC AGCGCTGTCA GCTCACCCGA TGGCGGCTCA 
GCCAGCGATT CAGGCGACCG ATTTCGTGCC GATCAATGCC GTTCAAGTCC TCAAGAGCCC 
AGCAAGATTG AGACCCTAAT CCGAGCCACA CAGCAGATGA TCAAAGAGGA GGAAAGTCGT 
CTGCAGCTGA GAAAGCTTCC TACAGACGTA CCACTGGAGC CCACAAACAG TCTGGCTAAG 
AGTTTTCATA GTACCGACTT TCCCCAGTCA GCAATGCAGA GTGTTGTGTG TCGAGGGCCG 
GCTCAGGTCA TAAGCCCCGC TCCTAGCCCC GTTCCCCTGT CCCGCCTAAG CAGCCCACTC 
CCGGACAGAC TATCTAAAGG CAAAGACTTC CTACAGAATG AGCTTTCCTC TTCCCAGCTG 
CCCCTGACCG GCACTTGTGC TGTTTCTCCG ACTCCTGCGC TTTACTCCTT ACATCCACGC 
CAGTACCTGG AAAAACACGC AGCGTACTCG CTCACCAGCT ACGCACTGGA ACACCTGTAT 
GAGGCGGACA GCTTTCGAGG ATACTCCCTT GGGTGCTCTG GTTCCTCTCA TTACGATATG 
ACCACTCATC TACGCATGCA GGCTGAACAA GCGCCGGGCC ATAAAGGCAC CTCCGTCATC 
ATCACCAATG GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835740.2
CDS	402..2639
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRIVFPEGLGESWGHVSRTTSLENVGRELGSHLLQTLDGFIFVVAPDGKILYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVHEYEMERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHSCDTFHLRCAHHLLLVKGQVTTKYYRFLAKQGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQAASTKPSFTYNSPSNPVTENRRVGKSRVSRTKTKTRLSPYSQYPGFPTDRSESDQDSPWGGSPLTDSASPQLLEQCEGIESSCVYRQFSDPRSLCYGLPLTEDHHTSNELYSHPHSESCERGCCKAGRYFLGTPQPGREAWWGAARSVLPLPKSSPENGDSFEGVMPHIASIHSLQVRGHWDEDSAVSSPDGGSASDSGDRFRADQCRSSPQEPSKIETLIRATQQMIKEEESRLQLRKLPTDVPLEPTNSLAKSFHSTDFPQSAMQSVVCRGPAQVISPAPSPVPLSRLSSPLPDRLSKGKDFLQNELSSSQLPLTGTCAVSPTPALYSLHPRQYLEKHAAYSLTSYALEHLYEADSFRGYSLGCSGSSHYDMTTHLRMQAEQAPGHKGTSVIITNGS

Target ORF information:

RefSeq Version	NM_178222.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SIM bHLH transcription factor 1a (sim1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178222.3

ORF Insert Sequence:

ATGAAGGAGA AGTCGAAAAA CGCGGCGCGC ACGCGGCGGG AGAAGGAAAA CAGCGAGTTT 
TACGAGCTCG CCAAACTGCT GCCTCTGCCC TCCGCCATCA CCTCACAGCT GGACAAAGCC 
TCCATCATCA GACTCACCAC CAGCTACCTG AAAATGAGGA TAGTCTTCCC TGAAGGTCTT 
GGGGAATCTT GGGGCCATGT GAGTCGAACG ACTTCACTGG AAAATGTCGG CCGAGAATTA 
GGATCACATC TGCTTCAGAC GTTAGACGGC TTCATCTTTG TGGTGGCTCC TGATGGGAAA 
ATCCTGTACA TTTCAGAAAC GGCGTCAGTG CATTTAGGCT TGTCACAGGT AGAGCTGACG 
GGGAACAGCA TTTATGAGTA CATCCACCCC GCAGACCACG ACGAAATGAC CGCAGTGCTG 
ACGGCTCACC AGCCCTATCA TTCACACTTC GTCCATGAGT ACGAAATGGA GCGCTCTTTT 
TTCCTGAGGA TGAAATGTGT CCTTGCTAAA CGTAACGCAG GATTGACGTG CGGTGGTTAC 
AAGGTCATCC ACTGCAGCGG GTACCTGAAG ATCCGTCAGT ACAGTTTAGA CATGTCTCCG 
TTCGACGGCT GCTATCAGAA TGTGGGTCTG GTGGCTGTAG GTCACTCGCT CCCGCCCAGC 
GCAGTCACCG AGATCAAACT GCACAGCAAC ATGTTCATGT TCAGAGCCAG CCTGGACATG 
AAGCTCATCT TCCTGGACTC CAGGGTTGCG GAGCTCACTG GCTACGAGCC GCAAGACTTA 
ATAGAGAAGA CACTTTATCA TCACGTCCAC AGCTGTGACA CTTTCCATCT CCGCTGCGCG 
CACCACTTGT TGCTGGTAAA AGGACAAGTC ACCACTAAAT ACTACCGATT CCTGGCGAAG 
CAGGGCGGAT GGGTGTGGGT ACAGAGTTAC GCCACCATCG TCCACAACAG CAGATCATCC 
AGACCTCACT GCATCGTCAG CGTCAATTAC GTCCTCACGG ACACGGAGTA CAAAGGACTA 
CAGTTGTCTT TGGACCAGGC GGCGTCCACC AAACCCTCCT TCACCTACAA CAGCCCCTCC 
AACCCCGTCA CCGAGAACAG AAGAGTTGGA AAAAGCAGAG TCTCCCGAAC CAAGACCAAA 
ACAAGACTCT CTCCGTACTC ACAGTATCCA GGTTTCCCCA CAGATCGCTC GGAGTCGGAT 
CAGGACAGCC CATGGGGTGG GAGTCCACTG ACTGATTCTG CATCTCCTCA GCTTCTGGAG 
CAGTGTGAAG GCATCGAGTC CTCATGCGTG TACCGGCAGT TCTCGGACCC CCGCTCGCTG 
TGCTATGGTT TACCCCTGAC GGAAGATCAC CACACCTCCA ACGAGCTCTA CAGCCACCCT 
CACTCGGAGT CCTGCGAGAG GGGCTGCTGT AAAGCCGGAC GCTACTTTCT GGGCACCCCT 
CAGCCCGGAC GAGAGGCATG GTGGGGCGCA GCCCGCTCGG TCCTCCCCTT ACCCAAATCT 
TCACCTGAGA ATGGGGACAG TTTTGAAGGC GTGATGCCTC ACATCGCTTC TATCCATAGC 
CTGCAAGTGA GGGGTCACTG GGATGAGGAC AGCGCTGTCA GCTCACCCGA TGGCGGCTCA 
GCCAGCGATT CAGGCGACCG ATTTCGTGCC GATCAATGCC GTTCAAGTCC TCAAGAGCCC 
AGCAAGATTG AGACCCTAAT CCGAGCCACA CAGCAGATGA TCAAAGAGGA GGAAAGTCGT 
CTGCAGCTGA GAAAGCTTCC TACAGACGTA CCACTGGAGC CCACAAACAG TCTGGCTAAG 
AGTTTTCATA GTACCGACTT TCCCCAGTCA GCAATGCAGA GTGTTGTGTG TCGAGGGCCG 
GCTCAGGTCA TAAGCCCCGC TCCTAGCCCC GTTCCCCTGT CCCGCCTAAG CAGCCCACTC 
CCGGACAGAC TATCTAAAGG CAAAGACTTC CTACAGAATG AGCTTTCCTC TTCCCAGCTG 
CCCCTGACCG GCACTTGTGC TGTTTCTCCG ACTCCTGCGC TTTACTCCTT ACATCCACGC 
CAGTACCTGG AAAAACACGC AGCGTACTCG CTCACCAGCT ACGCACTGGA ACACCTGTAT 
GAGGCGGACA GCTTTCGAGG ATACTCCCTT GGGTGCTCTG GTTCCTCTCA TTACGATATG 
ACCACTCATC TACGCATGCA GGCTGAACAA GCGCCGGGCC ATAAAGGCAC CTCCGTCATC 
ATCACCAATG GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Homeogene emx1 is required for nephron distal segment development in zebrafish.
Scientific reports8(1)18038(2018 12)
Morales EE,Handa N,Drummond BE,Chambers JM,Marra AN,Addiego A,Wingert RA

eLife7(2018 11)
Chambers JM,Poureetezadi SJ,Addiego A,Lahne M,Wingert RA

Separate roles for Med12 and Wnt signaling in regulation of oxytocin expression.
Biology open7(3)(2018 Mar)
Spikol ED,Glasgow E

Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid-Contacting KA Spinal Neurons.
Frontiers in neuroscience12170(2018)
Andrzejczuk LA,Banerjee S,England SJ,Voufo C,Kamara K,Lewis KE

Transcriptional Complexity and Distinct Expression Patterns of
G3 (Bethesda, Md.)7(8)2577-2593(2017 08)
Kondrychyn I,Robra L,Thirumalai V

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sim1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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