Sim1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sim1
Entrez Gene ID	309888
Full Name	single-minded family bHLH transcription factor 1
General protein information	Preferred Names single-minded family bHLH transcription factor 1 Names single-minded homolog 1 single-minded 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107641.1	NP_001101111.1	single-minded homolog 1
XM_008772994.2	XP_008771216.1	single-minded homolog 1 isoform X1
XM_008772998.2	XP_008771220.1	single-minded homolog 1 isoform X2
XM_008772995.2	XP_008771217.1	single-minded homolog 1 isoform X1
XM_008772996.1	XP_008771218.1	single-minded homolog 1 isoform X1
XM_006256604.2	XP_006256666.1	single-minded homolog 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIM1	NP_001074957.1
Danio rerio (zebrafish)	sim1a	NP_835740.1
Xenopus tropicalis (tropical clawed frog)	sim1	XP_004914602.1
Homo sapiens (human)	SIM1	NP_005059.2
Canis lupus familiaris (dog)	SIM1	XP_539058.2
Mus musculus (house mouse)	Sim1	NP_035506.2
Gallus gallus (chicken)	SIM1	XP_004940413.1
Macaca mulatta (Rhesus monkey)	SIM1	XP_001086054.1
Bos taurus (cattle)	SIM1	NP_001179901.1
Rattus norvegicus (Norway rat)	Sim1	NP_001101111.1

Gene Ontology
Bibliography

Related articles in PubMed

NPAS1-ARNT and NPAS3-ARNT crystal structures implicate the bHLH-PAS family as multi-ligand binding transcription factors.
Wu D, Su X, Potluri N, Kim Y, Rastinejad F
eLife5(2016 10)

Spatial gene expression in the T-stage mouse metanephros.
Caruana G, Cullen-McEwen L, Nelson AL, Kostoulias X, Woods K, Gardiner B, Davis MJ, Taylor DF, Teasdale RD, Grimmond SM, Little MH, Bertram JF
Gene expression patterns : GEP6(8)807-25(2006 Oct)

Differential activities of murine single minded 1 (SIM1) and SIM2 on a hypoxic response element. Cross-talk between basic helix-loop-helix/per-Arnt-Sim homology transcription factors.
Woods SL, Whitelaw ML
The Journal of biological chemistry277(12)10236-43(2002 Mar)

Profound obesity associated with a balanced translocation that disrupts the SIM1 gene.
Holder JL Jr, Butte NF, Zinn AR
Human molecular genetics9(1)101-8(2000 Jan)

Two new members of the murine Sim gene family are transcriptional repressors and show different expression patterns during mouse embryogenesis.
Ema M, Morita M, Ikawa S, Tanaka M, Matsuda Y, Gotoh O, Saijoh Y, Fujii H, Hamada H, Kikuchi Y, Fujii-Kuriyama Y
Molecular and cellular biology16(10)5865-75(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04177	NM_001107641.1 Latest version!	Rattus norvegicus SIM bHLH transcription factor 1 (Sim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa04177	XM_006256604.2 Latest version!	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa04177	XM_008772995.2 Latest version!	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa04177	XM_008772996.1 Latest version!	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa04177	XM_008772994.2 Latest version!	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa46803	XM_008772998.2 Latest version!	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sim1 ( NM_001107641.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101111.1 Rattus norvegicus SIM bHLH transcription factor 1 (Sim1), mRNA.

Price & Availability↑

CloneID	ORa04177
Clone ID Related Accession (Same CDS sequence)	NM_001107641.1 , XM_008772994.2 , XM_008772995.2 , XM_008772996.1 , XM_006256604.2
Accession Version	NM_001107641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	single-minded homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474025.1. On or before Oct 4, 2007 this sequence version replaced XM_228329.4, XM_001065493.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642747 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101111.1
CDS	431..2728
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHTSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISPSKPTFSYTSSSTPTITDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPERPGSQHELSCAYRQFPDRGSLCYGFALDHSRLVEDRHFHTQACEGGRCEAGRYFLGAPPAGRDPWWGSRAALPLTKASPESRETYENSMPHITSVHRLHGRGHWDEDSVVSSPDPGSASESGDRYRSEQYQNSPHEPSKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKKHSLCFANYQQPPPAGEVCHSSALTSTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPSSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	NM_001107641.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SIM bHLH transcription factor 1 (Sim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107641.1

ORF Insert Sequence:

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim1 ( XM_008772994.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771216.1 Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa04177
Clone ID Related Accession (Same CDS sequence)	NM_001107641.1 , XM_008772994.2 , XM_008772995.2 , XM_008772996.1 , XM_006256604.2
Accession Version	XM_008772994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	single-minded homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771216.1
CDS	557..2854
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHTSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISPSKPTFSYTSSSTPTITDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPERPGSQHELSCAYRQFPDRGSLCYGFALDHSRLVEDRHFHTQACEGGRCEAGRYFLGAPPAGRDPWWGSRAALPLTKASPESRETYENSMPHITSVHRLHGRGHWDEDSVVSSPDPGSASESGDRYRSEQYQNSPHEPSKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKKHSLCFANYQQPPPAGEVCHSSALTSTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPSSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	XM_008772994.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772994.2

ORF Insert Sequence:

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim1 ( XM_008772995.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771217.1 Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa04177
Clone ID Related Accession (Same CDS sequence)	NM_001107641.1 , XM_008772994.2 , XM_008772995.2 , XM_008772996.1 , XM_006256604.2
Accession Version	XM_008772995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	single-minded homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771217.1
CDS	1906..4203
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHTSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISPSKPTFSYTSSSTPTITDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPERPGSQHELSCAYRQFPDRGSLCYGFALDHSRLVEDRHFHTQACEGGRCEAGRYFLGAPPAGRDPWWGSRAALPLTKASPESRETYENSMPHITSVHRLHGRGHWDEDSVVSSPDPGSASESGDRYRSEQYQNSPHEPSKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKKHSLCFANYQQPPPAGEVCHSSALTSTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPSSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	XM_008772995.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772995.2

ORF Insert Sequence:

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim1 ( XM_008772996.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771218.1 Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa04177
Clone ID Related Accession (Same CDS sequence)	NM_001107641.1 , XM_008772994.2 , XM_008772995.2 , XM_008772996.1 , XM_006256604.2
Accession Version	XM_008772996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	single-minded homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771218.1
CDS	542..2839
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHTSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISPSKPTFSYTSSSTPTITDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPERPGSQHELSCAYRQFPDRGSLCYGFALDHSRLVEDRHFHTQACEGGRCEAGRYFLGAPPAGRDPWWGSRAALPLTKASPESRETYENSMPHITSVHRLHGRGHWDEDSVVSSPDPGSASESGDRYRSEQYQNSPHEPSKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKKHSLCFANYQQPPPAGEVCHSSALTSTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPSSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	XM_008772996.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772996.1

ORF Insert Sequence:

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim1 ( XM_006256604.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256666.1 Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa04177
Clone ID Related Accession (Same CDS sequence)	NM_001107641.1 , XM_008772994.2 , XM_008772995.2 , XM_008772996.1 , XM_006256604.2
Accession Version	XM_006256604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	single-minded homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006256604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256666.1
CDS	719..3016
Translation	MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHTSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISPSKPTFSYTSSSTPTITDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPERPGSQHELSCAYRQFPDRGSLCYGFALDHSRLVEDRHFHTQACEGGRCEAGRYFLGAPPAGRDPWWGSRAALPLTKASPESRETYENSMPHITSVHRLHGRGHWDEDSVVSSPDPGSASESGDRYRSEQYQNSPHEPSKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKKHSLCFANYQQPPPAGEVCHSSALTSTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPSSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	XM_006256604.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256604.2

ORF Insert Sequence:

ATGAAAGAGA AGTCTAAAAA TGCTGCCCGG ACGAGGAGGG AGAAAGAAAA CAGTGAATTT 
TACGAACTGG CTAAATTACT GCCGTTACCC TCAGCCATCA CCTCGCAACT GGACAAAGCC 
TCGATCATCA GACTCACGAC CAGCTATCTC AAAATGAGAG TGGTCTTTCC GGAAGGGCTT 
GGCGAGGCGT GGGGTCACAC CAGTCGAACC AGCCCTCTGG ATAACGTTGG TCGCGAACTC 
GGCTCTCATC TACTCCAGAC TCTGGATGGC TTCATCTTTG TGGTGGCCCC AGATGGGAAG 
ATCATGTACA TCTCAGAGAC AGCTTCAGTT CACCTGGGCC TTTCTCAGGT AGAACTGACT 
GGGAACAGCA TCTATGAATA TATCCACCCG GCTGATCATG ATGAGATGAC AGCAGTGCTC 
ACAGCCCATC AACCCTACCA CTCTCACTTT GTACAGGAGT ACGAGATCGA ACGCTCCTTC 
TTTCTAAGGA TGAAGTGTGT TTTGGCCAAG CGGAATGCTG GCCTCACCTG CGGTGGCTAC 
AAGGTCATTC ACTGTAGCGG CTACCTGAAG ATCCGCCAGT ACAGCCTGGA TATGTCTCCC 
TTTGATGGGT GCTACCAAAA TGTGGGTTTG GTGGCTGTGG GTCACTCCCT GCCTCCGAGC 
GCTGTGACAG AGATCAAACT ACACAGCAAC ATGTTCATGT TCCGAGCCAG TCTGGACATG 
AAACTCATTT TCCTGGACTC CAGGGTAGCA GAGCTGACTG GGTATGAACC TCAGGACTTG 
ATTGAGAAGA CCCTGTACCA TCACGTGCAC GGCTGTGACA CCTTCCACCT ACGCTGTGCA 
CACCATTTAC TGTTGGTGAA GGGTCAGGTG ACCACCAAGT ACTACAGGTT CTTGGCAAAA 
CACGGCGGCT GGGTCTGGGT GCAGAGTTAC GCCACCATCG TACACAACAG CCGCTCCTCC 
AGGCCGCACT GCATCGTCAG CGTTAACTAC GTCCTCACAG ACACAGAATA CAAAGGGCTG 
CAGCTCTCTC TGGACCAGAT ATCACCCTCT AAGCCCACTT TCTCCTATAC CAGCAGTTCC 
ACACCCACCA TCACTGACAA CAGGAAGGGG GCCAAGTCAA GGCTCTCTAG TAGCAAGTCA 
AAATCCAGGA CCTCCCCATA TCCCCAGTAT TCGGGATTCC ACACTGAAAG ATCAGAATCT 
GACCATGACA GCCAGTGGGG TGGAAGTCCC CTGACTGATA CTGCCTCCCC ACAGCTCCTG 
GACCCAGAGA GGCCTGGCTC TCAGCACGAG TTGTCCTGTG CGTACAGACA GTTTCCAGAT 
CGCGGTTCTC TCTGCTATGG CTTTGCGCTG GACCATTCCA GGCTGGTGGA GGACAGGCAC 
TTCCACACCC AAGCCTGCGA GGGAGGGCGG TGTGAGGCTG GCAGGTACTT TCTCGGAGCA 
CCACCTGCTG GGAGAGATCC CTGGTGGGGT TCTCGAGCAG CTCTGCCGCT CACCAAGGCC 
TCCCCGGAGA GCAGAGAAAC CTATGAGAAC AGCATGCCGC ACATCACCTC TGTCCACAGA 
CTCCACGGTC GAGGTCACTG GGATGAAGAT AGTGTGGTCA GCTCTCCAGA CCCTGGGTCA 
GCCAGTGAAT CCGGTGACCG ATACCGCTCC GAACAGTATC AAAATAGCCC ACATGAACCT 
AGCAAAATTG AAACTCTCAT AAGAGCCACC CAGCAGATGA TTAAAGAGGA AGAGAACAGA 
TTACAGCTAA GGAAAGCCCC TCCCGACCAA CTGGCTTCCA TTAACGGGGC TGGGAAGAAA 
CACTCCCTCT GTTTTGCAAA CTATCAACAG CCCCCACCGG CAGGTGAAGT CTGCCACAGC 
TCTGCTCTTA CCAGCACTTC ACCATGTGAC CACATCCAGC AGAGAGAGGG AAAGATGCTT 
AGCCCTCACG AAAACGACTA TGACAACAGT CCCACTGCAC TGTCTCGGAT AAGTAGTCCC 
AGTTCTGATC GCATAACAAA GTCCAGTTTA ATCCTGGCTA AAGACTATCT ACATTCAGAT 
ATGTCTCCTC ATCAGACAGC AGGAGACCAT CCTGCCATCT CTCCAAACTG TTTTGGCTCC 
CACCGGCAGT ATTTTGATAA GCATGCCTAT ACGTTAACTG GTTATGCCCT GGAACATTTA 
TATGACAGTG AGACTATTAG GAACTATTCC TTGGGCTGCA ATGGCTCACA CTTTGATGTA 
ACCTCCCATC TAAGGATGCA GCCGGACCCA GCGCAAGGAC ACAAGGGAAC ATCTGTTATA 
ATAACCAATG GAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sim1 ( XM_008772998.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771220.1 Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X5, mRNA.

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CloneID	ORa46803
Clone ID Related Accession (Same CDS sequence)	XM_008772998.2
Accession Version	XM_008772998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	single-minded homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCAG TGCTCACAGC CCATCAACCC TACCACTCTC ACTTTGTACA GGAGTACGAG 
ATCGAACGCT CCTTCTTTCT AAGGATGAAG TGTGTTTTGG CCAAGCGGAA TGCTGGCCTC 
ACCTGCGGTG GCTACAAGGT CATTCACTGT AGCGGCTACC TGAAGATCCG CCAGTACAGC 
CTGGATATGT CTCCCTTTGA TGGGTGCTAC CAAAATGTGG GTTTGGTGGC TGTGGGTCAC 
TCCCTGCCTC CGAGCGCTGT GACAGAGATC AAACTACACA GCAACATGTT CATGTTCCGA 
GCCAGTCTGG ACATGAAACT CATTTTCCTG GACTCCAGGG TAGCAGAGCT GACTGGGTAT 
GAACCTCAGG ACTTGATTGA GAAGACCCTG TACCATCACG TGCACGGCTG TGACACCTTC 
CACCTACGCT GTGCACACCA TTTACTGTTG GTGAAGGGTC AGGTGACCAC CAAGTACTAC 
AGGTTCTTGG CAAAACACGG CGGCTGGGTC TGGGTGCAGA GTTACGCCAC CATCGTACAC 
AACAGCCGCT CCTCCAGGCC GCACTGCATC GTCAGCGTTA ACTACGTCCT CACAGACACA 
GAATACAAAG GGCTGCAGCT CTCTCTGGAC CAGATATCAC CCTCTAAGCC CACTTTCTCC 
TATACCAGCA GTTCCACACC CACCATCACT GACAACAGGA AGGGGGCCAA GTCAAGGCTC 
TCTAGTAGCA AGTCAAAATC CAGGACCTCC CCATATCCCC AGTATTCGGG ATTCCACACT 
GAAAGATCAG AATCTGACCA TGACAGCCAG TGGGGTGGAA GTCCCCTGAC TGATACTGCC 
TCCCCACAGC TCCTGGACCC AGAGAGGCCT GGCTCTCAGC ACGAGTTGTC CTGTGCGTAC 
AGACAGTTTC CAGATCGCGG TTCTCTCTGC TATGGCTTTG CGCTGGACCA TTCCAGGCTG 
GTGGAGGACA GGCACTTCCA CACCCAAGCC TGCGAGGGAG GGCGGTGTGA GGCTGGCAGG 
TACTTTCTCG GAGCACCACC TGCTGGGAGA GATCCCTGGT GGGGTTCTCG AGCAGCTCTG 
CCGCTCACCA AGGCCTCCCC GGAGAGCAGA GAAACCTATG AGAACAGCAT GCCGCACATC 
ACCTCTGTCC ACAGACTCCA CGGTCGAGGT CACTGGGATG AAGATAGTGT GGTCAGCTCT 
CCAGACCCTG GGTCAGCCAG TGAATCCGGT GACCGATACC GCTCCGAACA GTATCAAAAT 
AGCCCACATG AACCTAGCAA AATTGAAACT CTCATAAGAG CCACCCAGCA GATGATTAAA 
GAGGAAGAGA ACAGATTACA GCTAAGGAAA GCCCCTCCCG ACCAACTGGC TTCCATTAAC 
GGGGCTGGGA AGAAACACTC CCTCTGTTTT GCAAACTATC AACAGCCCCC ACCGGCAGGT 
GAAGTCTGCC ACAGCTCTGC TCTTACCAGC ACTTCACCAT GTGACCACAT CCAGCAGAGA 
GAGGGAAAGA TGCTTAGCCC TCACGAAAAC GACTATGACA ACAGTCCCAC TGCACTGTCT 
CGGATAAGTA GTCCCAGTTC TGATCGCATA ACAAAGTCCA GTTTAATCCT GGCTAAAGAC 
TATCTACATT CAGATATGTC TCCTCATCAG ACAGCAGGAG ACCATCCTGC CATCTCTCCA 
AACTGTTTTG GCTCCCACCG GCAGTATTTT GATAAGCATG CCTATACGTT AACTGGTTAT 
GCCCTGGAAC ATTTATATGA CAGTGAGACT ATTAGGAACT ATTCCTTGGG CTGCAATGGC 
TCACACTTTG ATGTAACCTC CCATCTAAGG ATGCAGCCGG ACCCAGCGCA AGGACACAAG 
GGAACATCTG TTATAATAAC CAATGGAAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771220.1
CDS	139..2031
Translation	MTAVLTAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISPSKPTFSYTSSSTPTITDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLLDPERPGSQHELSCAYRQFPDRGSLCYGFALDHSRLVEDRHFHTQACEGGRCEAGRYFLGAPPAGRDPWWGSRAALPLTKASPESRETYENSMPHITSVHRLHGRGHWDEDSVVSSPDPGSASESGDRYRSEQYQNSPHEPSKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKKHSLCFANYQQPPPAGEVCHSSALTSTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPSSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS

Target ORF information:

RefSeq Version	XM_008772998.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus single-minded family bHLH transcription factor 1 (Sim1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772998.2

ORF Insert Sequence:

ATGACAGCAG TGCTCACAGC CCATCAACCC TACCACTCTC ACTTTGTACA GGAGTACGAG 
ATCGAACGCT CCTTCTTTCT AAGGATGAAG TGTGTTTTGG CCAAGCGGAA TGCTGGCCTC 
ACCTGCGGTG GCTACAAGGT CATTCACTGT AGCGGCTACC TGAAGATCCG CCAGTACAGC 
CTGGATATGT CTCCCTTTGA TGGGTGCTAC CAAAATGTGG GTTTGGTGGC TGTGGGTCAC 
TCCCTGCCTC CGAGCGCTGT GACAGAGATC AAACTACACA GCAACATGTT CATGTTCCGA 
GCCAGTCTGG ACATGAAACT CATTTTCCTG GACTCCAGGG TAGCAGAGCT GACTGGGTAT 
GAACCTCAGG ACTTGATTGA GAAGACCCTG TACCATCACG TGCACGGCTG TGACACCTTC 
CACCTACGCT GTGCACACCA TTTACTGTTG GTGAAGGGTC AGGTGACCAC CAAGTACTAC 
AGGTTCTTGG CAAAACACGG CGGCTGGGTC TGGGTGCAGA GTTACGCCAC CATCGTACAC 
AACAGCCGCT CCTCCAGGCC GCACTGCATC GTCAGCGTTA ACTACGTCCT CACAGACACA 
GAATACAAAG GGCTGCAGCT CTCTCTGGAC CAGATATCAC CCTCTAAGCC CACTTTCTCC 
TATACCAGCA GTTCCACACC CACCATCACT GACAACAGGA AGGGGGCCAA GTCAAGGCTC 
TCTAGTAGCA AGTCAAAATC CAGGACCTCC CCATATCCCC AGTATTCGGG ATTCCACACT 
GAAAGATCAG AATCTGACCA TGACAGCCAG TGGGGTGGAA GTCCCCTGAC TGATACTGCC 
TCCCCACAGC TCCTGGACCC AGAGAGGCCT GGCTCTCAGC ACGAGTTGTC CTGTGCGTAC 
AGACAGTTTC CAGATCGCGG TTCTCTCTGC TATGGCTTTG CGCTGGACCA TTCCAGGCTG 
GTGGAGGACA GGCACTTCCA CACCCAAGCC TGCGAGGGAG GGCGGTGTGA GGCTGGCAGG 
TACTTTCTCG GAGCACCACC TGCTGGGAGA GATCCCTGGT GGGGTTCTCG AGCAGCTCTG 
CCGCTCACCA AGGCCTCCCC GGAGAGCAGA GAAACCTATG AGAACAGCAT GCCGCACATC 
ACCTCTGTCC ACAGACTCCA CGGTCGAGGT CACTGGGATG AAGATAGTGT GGTCAGCTCT 
CCAGACCCTG GGTCAGCCAG TGAATCCGGT GACCGATACC GCTCCGAACA GTATCAAAAT 
AGCCCACATG AACCTAGCAA AATTGAAACT CTCATAAGAG CCACCCAGCA GATGATTAAA 
GAGGAAGAGA ACAGATTACA GCTAAGGAAA GCCCCTCCCG ACCAACTGGC TTCCATTAAC 
GGGGCTGGGA AGAAACACTC CCTCTGTTTT GCAAACTATC AACAGCCCCC ACCGGCAGGT 
GAAGTCTGCC ACAGCTCTGC TCTTACCAGC ACTTCACCAT GTGACCACAT CCAGCAGAGA 
GAGGGAAAGA TGCTTAGCCC TCACGAAAAC GACTATGACA ACAGTCCCAC TGCACTGTCT 
CGGATAAGTA GTCCCAGTTC TGATCGCATA ACAAAGTCCA GTTTAATCCT GGCTAAAGAC 
TATCTACATT CAGATATGTC TCCTCATCAG ACAGCAGGAG ACCATCCTGC CATCTCTCCA 
AACTGTTTTG GCTCCCACCG GCAGTATTTT GATAAGCATG CCTATACGTT AACTGGTTAT 
GCCCTGGAAC ATTTATATGA CAGTGAGACT ATTAGGAACT ATTCCTTGGG CTGCAATGGC 
TCACACTTTG ATGTAACCTC CCATCTAAGG ATGCAGCCGG ACCCAGCGCA AGGACACAAG 
GGAACATCTG TTATAATAAC CAATGGAAGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NPAS1-ARNT and NPAS3-ARNT crystal structures implicate the bHLH-PAS family as multi-ligand binding transcription factors.
eLife5(2016 10)
Wu D,Su X,Potluri N,Kim Y,Rastinejad F

Spatial gene expression in the T-stage mouse metanephros.
Gene expression patterns : GEP6(8)807-25(2006 Oct)
Caruana G,Cullen-McEwen L,Nelson AL,Kostoulias X,Woods K,Gardiner B,Davis MJ,Taylor DF,Teasdale RD,Grimmond SM,Little MH,Bertram JF

Differential activities of murine single minded 1 (SIM1) and SIM2 on a hypoxic response element. Cross-talk between basic helix-loop-helix/per-Arnt-Sim homology transcription factors.
The Journal of biological chemistry277(12)10236-43(2002 Mar)
Woods SL,Whitelaw ML

Profound obesity associated with a balanced translocation that disrupts the SIM1 gene.
Human molecular genetics9(1)101-8(2000 Jan)
Holder JL Jr,Butte NF,Zinn AR

Two new members of the murine Sim gene family are transcriptional repressors and show different expression patterns during mouse embryogenesis.
Molecular and cellular biology16(10)5865-75(1996 Oct)
Ema M,Morita M,Ikawa S,Tanaka M,Matsuda Y,Gotoh O,Saijoh Y,Fujii H,Hamada H,Kikuchi Y,Fujii-Kuriyama Y

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Sim1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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