guca1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	guca1b
Entrez Gene ID	140431
Full Name	guanylate cyclase activator 1B
Synonyms	gcap2
General protein information	Preferred Names guanylate cyclase activator 1B Names guanylyl cyclase-activating protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131871.1	NP_571946.1	guanylyl cyclase-activating protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GUCA1B	XP_527386.2
Gallus gallus (chicken)	GUCA1B	NP_001007882.1
Canis lupus familiaris (dog)	GUCA1B	XP_538922.1
Danio rerio (zebrafish)	guca1b	NP_571946.1
Xenopus tropicalis (tropical clawed frog)	LOC100489509	XP_002933553.1
Homo sapiens (human)	GUCA1B	NP_002089.4
Bos taurus (cattle)	GUCA1B	NP_777211.1
Mus musculus (house mouse)	Guca1b	NP_666191.1
Rattus norvegicus (Norway rat)	Guca1b	NP_001101668.1
Macaca mulatta (Rhesus monkey)	GUCA1B	XP_001086988.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural Characterization of Ferrous Ion Binding to Retinal Guanylate Cyclase Activator Protein 5 from Zebrafish Photoreceptors.
Lim S, Scholten A, Manchala G, Cudia D, Zlomke-Sell SK, Koch KW, Ames JB
Biochemistry56(51)6652-6661(2017 12)

Lxr regulates lipid metabolic and visual perception pathways during zebrafish development.
Pinto CL, Kalasekar SM, McCollum CW, Riu A, Jonsson P, Lopez J, Swindell EC, Bouhlatouf A, Balaguer P, Bondesson M, Gustafsson JÅ
Molecular and cellular endocrinology41929-43(2016 Jan)

Retina specific GCAPs in zebrafish acquire functional selectivity in Ca2+-sensing by myristoylation and Mg2+-binding.
Sulmann S, Vocke F, Scholten A, Koch KW
Scientific reports511228(2015 Jun)

Differential calcium signaling by cone specific guanylate cyclase-activating proteins from the zebrafish retina.
Scholten A, Koch KW
PloS one6(8)e23117(2011)

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
Sato Y, Hashiguchi Y, Nishida M
BMC evolutionary biology9127(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following guca1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the guca1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34481	NM_131871.1 Latest version!	Danio rerio guanylate cyclase activator 1B (guca1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

guca1b ( NM_131871.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571946.1 Danio rerio guanylate cyclase activator 1B (guca1b), mRNA.

Price & Availability↑

CloneID	ODa34481
Clone ID Related Accession (Same CDS sequence)	NM_131871.1
Accession Version	NM_131871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526745600000
Organism	Danio rerio(zebrafish)
Product	guanylyl cyclase-activating protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY050502.1. ##Evidence-Data-START## Transcript exon combination :: AY050502.1, AY044456.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476731, SAMEA4476793 [ECO:0000348] ##Evidence-Data-END##

ATGGGTCAGC GGCTCAGCGA TGATTCGGAC GAAAAAGAGA TTGATGTTGC TGAACTACAG 
GAGTGGTATA AGAAGTTTGT GATTGAATGT CCGAGTGGAA CCCTCTTCAT GCACGATTTC 
AAGAGCTTTT TCGGTGTTAC TGAAAACCCG GAAGCAGCGG ATTACATTGA AAACATGTTC 
CGGGCTTTTG ATAAAAATGG GGACAATACT ATTGATTTTC TGGAATACGT GGCGGCCTTG 
AATCTAGTGC TGCGAGGGAA GTTGGAGCAC AAACTGAAAT GGACATTTAA AATGTATGAC 
AAGGATGGAA GCGGTTGCAT CGACAAAACA GAGCTCAAGG AGATTGTGGA GTCAATCTAC 
AGACTAAAAA AAGCCTGCCA TGGTGAACTC GATGCAGAAT GTAACCTGCT TACCCCAGAT 
CAGGTGGTGG ACAGGATATT TGAGCTGGTG GATGAGAACG GAGATGGAGA GCTTTCTCTG 
GATGAGTTCA TTGACGGGGC TCGGCGTGAC AAGTGGGTCA TGAAGATGCT GCAAATGGAC 
GTGAACCCCG GGGACTGGAT CAATGAACAG AGACGCCGCA GCGCCAACTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571946.1
CDS	232..825
Translation	MGQRLSDDSDEKEIDVAELQEWYKKFVIECPSGTLFMHDFKSFFGVTENPEAADYIENMFRAFDKNGDNTIDFLEYVAALNLVLRGKLEHKLKWTFKMYDKDGSGCIDKTELKEIVESIYRLKKACHGELDAECNLLTPDQVVDRIFELVDENGDGELSLDEFIDGARRDKWVMKMLQMDVNPGDWINEQRRRSANF

Target ORF information:

RefSeq Version	NM_131871.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanylate cyclase activator 1B (guca1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131871.1

ORF Insert Sequence:

ATGGGTCAGC GGCTCAGCGA TGATTCGGAC GAAAAAGAGA TTGATGTTGC TGAACTACAG 
GAGTGGTATA AGAAGTTTGT GATTGAATGT CCGAGTGGAA CCCTCTTCAT GCACGATTTC 
AAGAGCTTTT TCGGTGTTAC TGAAAACCCG GAAGCAGCGG ATTACATTGA AAACATGTTC 
CGGGCTTTTG ATAAAAATGG GGACAATACT ATTGATTTTC TGGAATACGT GGCGGCCTTG 
AATCTAGTGC TGCGAGGGAA GTTGGAGCAC AAACTGAAAT GGACATTTAA AATGTATGAC 
AAGGATGGAA GCGGTTGCAT CGACAAAACA GAGCTCAAGG AGATTGTGGA GTCAATCTAC 
AGACTAAAAA AAGCCTGCCA TGGTGAACTC GATGCAGAAT GTAACCTGCT TACCCCAGAT 
CAGGTGGTGG ACAGGATATT TGAGCTGGTG GATGAGAACG GAGATGGAGA GCTTTCTCTG 
GATGAGTTCA TTGACGGGGC TCGGCGTGAC AAGTGGGTCA TGAAGATGCT GCAAATGGAC 
GTGAACCCCG GGGACTGGAT CAATGAACAG AGACGCCGCA GCGCCAACTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural Characterization of Ferrous Ion Binding to Retinal Guanylate Cyclase Activator Protein 5 from Zebrafish Photoreceptors.
Biochemistry56(51)6652-6661(2017 12)
Lim S,Scholten A,Manchala G,Cudia D,Zlomke-Sell SK,Koch KW,Ames JB

Lxr regulates lipid metabolic and visual perception pathways during zebrafish development.
Molecular and cellular endocrinology41929-43(2016 Jan)
Pinto CL,Kalasekar SM,McCollum CW,Riu A,Jonsson P,Lopez J,Swindell EC,Bouhlatouf A,Balaguer P,Bondesson M,Gustafsson JÅ

Retina specific GCAPs in zebrafish acquire functional selectivity in Ca2+-sensing by myristoylation and Mg2+-binding.
Scientific reports511228(2015 Jun)
Sulmann S,Vocke F,Scholten A,Koch KW

Differential calcium signaling by cone specific guanylate cyclase-activating proteins from the zebrafish retina.
PloS one6(8)e23117(2011)
Scholten A,Koch KW

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
BMC evolutionary biology9127(2009 Jun)
Sato Y,Hashiguchi Y,Nishida M