ago4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ago4
Entrez Gene ID	100535764
Full Name	argonaute RISC catalytic component 4
Synonyms	eif2c4,fd14f04,wu:fd14f04
General protein information	Preferred Names argonaute RISC catalytic component 4 Names protein argonaute-4 argonaute 4 eukaryotic translation initiation factor 2C, 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001252559.2	NP_001239488.1	protein argonaute-4

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0729300	NP_001058648.1
Pan troglodytes (chimpanzee)	EIF2C4	XP_524663.3
Bos taurus (cattle)	AGO4	XP_002686598.2
Arabidopsis thaliana (thale cress)	ZLL	NP_199194.1
Danio rerio (zebrafish)	ago4	NP_001239488.1
Macaca mulatta (Rhesus monkey)	EIF2C4	XP_002802359.1
Mus musculus (house mouse)	Ago4	NP_694817.2
Oryza sativa (rice)	Os02g0831600	NP_001048621.1
Homo sapiens (human)	AGO4	NP_060099.2
Gallus gallus (chicken)	AGO4	NP_001034365.1
Canis lupus familiaris (dog)	AGO4	XP_005629018.1
Xenopus tropicalis (tropical clawed frog)	LOC100495218	XP_004911629.1

Gene Ontology
Bibliography

Related articles in PubMed

A Nuclear Role for miR-9 and Argonaute Proteins in Balancing Quiescent and Activated Neural Stem Cell States.
Katz S, Cussigh D, Urbán N, Blomfield I, Guillemot F, Bally-Cuif L, Coolen M
Cell reports17(5)1383-1398(2016 10)

Expansion of the Ago gene family in the teleost clade.
McFarlane L, Svingen T, Braasch I, Koopman P, Schartl M, Wilhelm D
Development genes and evolution221(2)95-104(2011 Jun)

A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity.
Cifuentes D, Xue H, Taylor DW, Patnode H, Mishima Y, Cheloufi S, Ma E, Mane S, Hannon GJ, Lawson ND, Wolfe SA, Giraldez AJ
Science (New York, N.Y.)328(5986)1694-8(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ago4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ago4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa50230	NM_001252559.2 Latest version!	Danio rerio argonaute RISC component 4 (ago4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ago4 ( NM_001252559.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001239488.1 Danio rerio argonaute RISC component 4 (ago4), mRNA.

Price & Availability↑

CloneID	ODa50230
Clone ID Related Accession (Same CDS sequence)	NM_001252559.2
Accession Version	NM_001252559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554566400000
Organism	Danio rerio(zebrafish)
Product	protein argonaute-4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from JX448395.1. On Dec 24, 2014 this sequence version replaced NM_001252559.1. ##Evidence-Data-START## Transcript exon combination :: JX448395.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGCGC TCGGACCCGG CCCGCCTGCC CCTACCTCCC TCTTCCAGCC ACCGCGCCGG 
CCCGGCTTGG GTACGGTGGG GAAGCCGATT CGCCTGCTGG CCAATCACTT CCAGGTGCAG 
ATCCCCAAGA TCGACGTCTA CCATTATGAC ATCGACATCA AACCTGAAAA ACGGCCCCGC 
AGGGTCAACA GAGAGGTGGT GGACACAATG GTTAGACATT TTAAGATGCA GATCTTTGGG 
GACCGGCAGC CTGGATATGA TGGAAAGAGG AACATGTACA CGGCACATCC GCTACCCATC 
GGGAGGGACA GGGTGGATCT GGAGGTGACG CTGCCGGGTG AAGGGAAGGA TCAGACTTTT 
AAGGTGTCTC TGCAGTGGGT GTCGGTGGTC AGTCTGCAGA TGCTACTGGA AGCTCTGTCT 
GGTCACCTGA ACGAGGTGCC GGAGGATTCA GTTCAGGCTC TGGACGTCAT CACTCGTCAC 
CTGCCTTCCA TGCGGTACAC TCCAGTAGGC CGGTCCTTCT TCTCTCCACC AGAGGGCTAC 
TATCATCCAC TGGGAGGAGG GAGGGAGGTG TGGTTCGGTT TCCATCAGTC TGTCCGTCCT 
GCCATGTGGA ACATGATGCT CAACATAGAT GTGTCAGCAA CAGCGTTTTA CAGAGCTCAG 
CCTGTCATCG AATTCATGTG TGAGGTTTTG GACATCCAGA ACATCAACGA GCAGACCAAA 
CCCCTCACAG ATTCACAACG TGTCAAGTTC ACCAAGGAGA TCCGAGGACT GAAAGTGGAG 
GTCACACACT GCGGCCAGAT GAAGAGGAAG TACCGTGTGT GTAATGTCAC GCGCCGCCCA 
GCCAGTCATC AGACGTTTCC TTTGCAGCTT GAGAATGGAC AAGCTATGGA GTGCACCGTT 
GCCCAGTATT TCAAACAAAA GTACAGCCTG CAGCTCAAAT ACCCCCACCT GCCCTGCCTC 
CAGGTGGGAC AGGAGCAGAA GCACACGTAC CTTCCCCTGG AGGTTTGTAA TATAGTGGCA 
GGACAGCGCT GCATAAAGAA ACTAACAGAT AATCAGACCT CCACCATGAT CAAAGCTACG 
GCCCGCTCAG CCCCAGACAG ACAGGAGGAG ATCAGCCGAC TGGTCAAAAG CAACAGTATG 
GTTGGCGGGC CCGACCCTTA TCTGAAAGAG TTTGGCATTG TGGTGCACAA TGACATGACC 
GAGGTGACAG GGCGGGTCCT CCCAGCGCCC ATGCTGCAGT ATGGGGGCCG GAATAAGACG 
GTGGCCACAC CCAACCAGGG CGTCTGGGAC ATGAGGGGGA AGCAGTTTTA TGCCGGAATA 
GAGATCAAGG TTTGGGCCGT GGCCTGCTTC GCCCCTCAGA AACAATGCAG AGAGGACCTG 
CTCAAGAGTT TCACTGACCA GCTGCGCAAG ATTTCCAAGG ATGCTGGGAT GCCGATCCAA 
GGCCAGCCGT GCTTCTGCAA ATACGCCCAG GGTGCAGACA GTGTGGAGCC CATGTTCAAG 
CATCTCAAGA TGTCTTACGT GGGCCTGCAG CTCATCGTGG TCATCCTGCC CGGAAAAACA 
CCTGTCTATG CGGAGGTGAA GCGTGTGGGA GACACTCTCC TGGGAATGGC CACTCAGTGT 
GTTCAGGTGA AGAACGTGGT AAAAACGTCT CCTCAGACGC TCTCCAACCT CTGTCTGAAG 
ATCAACGCCA AACTAGGAGG CATCAACAAT GTGCTGGTGC CACATCAGAG GCCTTCTGTG 
TTCCAGCAGC CGGTGATCTT CTTGGGTGCG GATGTCACTC ACCCTCCAGC GGGCGATGGG 
AAGAAGCCGT CCATCGCTGC AGTGGTAGGT AGTATGGACG GACACCCGAG CCGATACTGC 
GCCACCGTAC GGGTGCAAAC GTCCCGCCAG GATCTGTCCC AGGAGCAGCT CTTCAGCCAG 
GAGGTCATCC AGGATCTCAC CAACATGGTG CGCGAGCTGC TCATTCAGTT CTACAAATCC 
ACCCGATTCA AGCCCACGCG CATTATCTAC TACCGCGGAG GAGTTTCTGA GGGCCAGATG 
AAGCAGGTGG CGTGGCCAGA GCTGATCGCC ATCAGGAAAG CCTGCATCAG TCTAGAAGAG 
GATTACAGGC CGGGAATCAC ATACATCGTA GTGCAGAAAC GCCACCACAC TCGACTCTTC 
TGCTCCGACA AAGCCGAGAG GGTTGGGAAG AGTGGCAACG TCCCAGCAGG CACTACGGTG 
GACAGCACCA TCACACACCC CTCAGAGTTT GACTTCTACC TGTGCAGCCA TGCTGGCATC 
CAGGGCACCA GCCGTCCCTC TCACTATCAC GTGTTGTGGG ACGACAACTG TTTCACCGCT 
GACGAGCTCC AGCTTTTGAC CTATCAGCTC TGCCACACTT ATGTGCGCTG CACCCGCTCC 
GTCTCCATCC CCGCGCCAGC TTACTATGCC CGGCTAGTGG CCTTCCGTGC ACGCTATCAT 
TTAGTGGACA AAGACCATGA CAGTGCTGAA GGCAGTCACG TATCAGGACA GAGCAACGGT 
CGAGACCCGC AGGCGCTGGC GAAAGCTGTG CAGATTCACT ACGACACACA GCACACCATG 
TACTTCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239488.1
CDS	124..2715
Translation	MEALGPGPPAPTSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDLSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA

Target ORF information:

RefSeq Version	NM_001252559.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio argonaute RISC component 4 (ago4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252559.2

ORF Insert Sequence:

ATGGAAGCGC TCGGACCCGG CCCGCCTGCC CCTACCTCCC TCTTCCAGCC ACCGCGCCGG 
CCCGGCTTGG GTACGGTGGG GAAGCCGATT CGCCTGCTGG CCAATCACTT CCAGGTGCAG 
ATCCCCAAGA TCGACGTCTA CCATTATGAC ATCGACATCA AACCTGAAAA ACGGCCCCGC 
AGGGTCAACA GAGAGGTGGT GGACACAATG GTTAGACATT TTAAGATGCA GATCTTTGGG 
GACCGGCAGC CTGGATATGA TGGAAAGAGG AACATGTACA CGGCACATCC GCTACCCATC 
GGGAGGGACA GGGTGGATCT GGAGGTGACG CTGCCGGGTG AAGGGAAGGA TCAGACTTTT 
AAGGTGTCTC TGCAGTGGGT GTCGGTGGTC AGTCTGCAGA TGCTACTGGA AGCTCTGTCT 
GGTCACCTGA ACGAGGTGCC GGAGGATTCA GTTCAGGCTC TGGACGTCAT CACTCGTCAC 
CTGCCTTCCA TGCGGTACAC TCCAGTAGGC CGGTCCTTCT TCTCTCCACC AGAGGGCTAC 
TATCATCCAC TGGGAGGAGG GAGGGAGGTG TGGTTCGGTT TCCATCAGTC TGTCCGTCCT 
GCCATGTGGA ACATGATGCT CAACATAGAT GTGTCAGCAA CAGCGTTTTA CAGAGCTCAG 
CCTGTCATCG AATTCATGTG TGAGGTTTTG GACATCCAGA ACATCAACGA GCAGACCAAA 
CCCCTCACAG ATTCACAACG TGTCAAGTTC ACCAAGGAGA TCCGAGGACT GAAAGTGGAG 
GTCACACACT GCGGCCAGAT GAAGAGGAAG TACCGTGTGT GTAATGTCAC GCGCCGCCCA 
GCCAGTCATC AGACGTTTCC TTTGCAGCTT GAGAATGGAC AAGCTATGGA GTGCACCGTT 
GCCCAGTATT TCAAACAAAA GTACAGCCTG CAGCTCAAAT ACCCCCACCT GCCCTGCCTC 
CAGGTGGGAC AGGAGCAGAA GCACACGTAC CTTCCCCTGG AGGTTTGTAA TATAGTGGCA 
GGACAGCGCT GCATAAAGAA ACTAACAGAT AATCAGACCT CCACCATGAT CAAAGCTACG 
GCCCGCTCAG CCCCAGACAG ACAGGAGGAG ATCAGCCGAC TGGTCAAAAG CAACAGTATG 
GTTGGCGGGC CCGACCCTTA TCTGAAAGAG TTTGGCATTG TGGTGCACAA TGACATGACC 
GAGGTGACAG GGCGGGTCCT CCCAGCGCCC ATGCTGCAGT ATGGGGGCCG GAATAAGACG 
GTGGCCACAC CCAACCAGGG CGTCTGGGAC ATGAGGGGGA AGCAGTTTTA TGCCGGAATA 
GAGATCAAGG TTTGGGCCGT GGCCTGCTTC GCCCCTCAGA AACAATGCAG AGAGGACCTG 
CTCAAGAGTT TCACTGACCA GCTGCGCAAG ATTTCCAAGG ATGCTGGGAT GCCGATCCAA 
GGCCAGCCGT GCTTCTGCAA ATACGCCCAG GGTGCAGACA GTGTGGAGCC CATGTTCAAG 
CATCTCAAGA TGTCTTACGT GGGCCTGCAG CTCATCGTGG TCATCCTGCC CGGAAAAACA 
CCTGTCTATG CGGAGGTGAA GCGTGTGGGA GACACTCTCC TGGGAATGGC CACTCAGTGT 
GTTCAGGTGA AGAACGTGGT AAAAACGTCT CCTCAGACGC TCTCCAACCT CTGTCTGAAG 
ATCAACGCCA AACTAGGAGG CATCAACAAT GTGCTGGTGC CACATCAGAG GCCTTCTGTG 
TTCCAGCAGC CGGTGATCTT CTTGGGTGCG GATGTCACTC ACCCTCCAGC GGGCGATGGG 
AAGAAGCCGT CCATCGCTGC AGTGGTAGGT AGTATGGACG GACACCCGAG CCGATACTGC 
GCCACCGTAC GGGTGCAAAC GTCCCGCCAG GATCTGTCCC AGGAGCAGCT CTTCAGCCAG 
GAGGTCATCC AGGATCTCAC CAACATGGTG CGCGAGCTGC TCATTCAGTT CTACAAATCC 
ACCCGATTCA AGCCCACGCG CATTATCTAC TACCGCGGAG GAGTTTCTGA GGGCCAGATG 
AAGCAGGTGG CGTGGCCAGA GCTGATCGCC ATCAGGAAAG CCTGCATCAG TCTAGAAGAG 
GATTACAGGC CGGGAATCAC ATACATCGTA GTGCAGAAAC GCCACCACAC TCGACTCTTC 
TGCTCCGACA AAGCCGAGAG GGTTGGGAAG AGTGGCAACG TCCCAGCAGG CACTACGGTG 
GACAGCACCA TCACACACCC CTCAGAGTTT GACTTCTACC TGTGCAGCCA TGCTGGCATC 
CAGGGCACCA GCCGTCCCTC TCACTATCAC GTGTTGTGGG ACGACAACTG TTTCACCGCT 
GACGAGCTCC AGCTTTTGAC CTATCAGCTC TGCCACACTT ATGTGCGCTG CACCCGCTCC 
GTCTCCATCC CCGCGCCAGC TTACTATGCC CGGCTAGTGG CCTTCCGTGC ACGCTATCAT 
TTAGTGGACA AAGACCATGA CAGTGCTGAA GGCAGTCACG TATCAGGACA GAGCAACGGT 
CGAGACCCGC AGGCGCTGGC GAAAGCTGTG CAGATTCACT ACGACACACA GCACACCATG 
TACTTCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Nuclear Role for miR-9 and Argonaute Proteins in Balancing Quiescent and Activated Neural Stem Cell States.
Cell reports17(5)1383-1398(2016 10)
Katz S,Cussigh D,Urbán N,Blomfield I,Guillemot F,Bally-Cuif L,Coolen M

Expansion of the Ago gene family in the teleost clade.
Development genes and evolution221(2)95-104(2011 Jun)
McFarlane L,Svingen T,Braasch I,Koopman P,Schartl M,Wilhelm D

A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity.
Science (New York, N.Y.)328(5986)1694-8(2010 Jun)
Cifuentes D,Xue H,Taylor DW,Patnode H,Mishima Y,Cheloufi S,Ma E,Mane S,Hannon GJ,Lawson ND,Wolfe SA,Giraldez AJ

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ago4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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