MAP3K1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K1
Entrez Gene ID	100334486
Full Name	mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
Synonyms	si:rp71-80a19.2
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase Names mitogen-activated protein kinase kinase kinase 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155507.4	XP_005155564.1	mitogen-activated protein kinase kinase kinase 1
XM_005155507.3	XP_005155564.1	mitogen-activated protein kinase kinase kinase 1 isoform X1
XM_009305108.2	XP_009303383.1	mitogen-activated protein kinase kinase kinase 1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP3K1	NP_005912.1
Rattus norvegicus (Norway rat)	LOC100912399	XP_006231988.1
Macaca mulatta (Rhesus monkey)	MAP3K1	XP_002804414.1
Canis lupus familiaris (dog)	MAP3K1	XP_535240.4
Xenopus tropicalis (tropical clawed frog)	map3k1	XP_002940279.2
Rattus norvegicus (Norway rat)	Map3k1	NP_446339.1
Pan troglodytes (chimpanzee)	MAP3K1	XP_003310777.1
Bos taurus (cattle)	MAP3K1	NP_001192835.1
Mus musculus (house mouse)	Map3k1	NP_036075.2
Gallus gallus (chicken)	MAP3K1	XP_424734.3
Danio rerio (zebrafish)	map3k1	XP_002663531.2

Gene Ontology

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15816	XM_005155507.4 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa49967	XM_009305108.2 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
ODa15816	XM_005155507.3	Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K1 ( XM_005155507.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155564.1 Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), mRNA.

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CloneID	ODa15816
Clone ID Related Accession (Same CDS sequence)	XM_005155507.4 , XM_005155507.3
Accession Version	XM_005155507.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155507.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGG CGGGGAGCCG GACGTCGTCG TTAGGCGGAT TATTAGACTC CAGTAGCGGC 
ACAAACGTCG GGAACGGACT TCAAGGCACT TCCAACGGCT CCACGTCTCA GAACAATAGC 
GGGAAAGTGA AGAGAAATGC GGGTGAGAGC TCTCAGTGGA GACGAAAGGT GAGGAGTGTG 
GATCTGGATA AATGCGAGAG CGGGACCGCA GCGCTGCACT TCCTCACGGT GTCCGGTCCC 
GGCTCGATCC AGACCGCTTT ACTGCAGCAG AGCCTCGCCG ACCCGCCGCT CGGTGACAAA 
ATAAGCAATA ATTTGTGTAC AAGTGCTCAG GAATGGATGA AAAGCTCTTT AAAAAGCGGC 
AGTGAAAGTC CGAGCGCTGA ACATGGAGGG GCTGCTGCTG CTGTGGACGT GAACGCTTCG 
CAAAACAGCC GGGACATGGA GAACAAGGAG ACTCTGCGCG GGCTGCAGAA GATGGAGGAC 
CGGCCTGAGG AGCGACTGAT CCGAGAGAAG CTCAAGGCCA CCTGTATGCC CACCTGGAAG 
AGCGAGTGGC TGGAGAGGCG GAGCAGGAGA GGACCAATGG TTGTGAAGCC CATTCCTCTG 
AGAGCCGACG GCACTGAAGC CGGGAGTGAC AGTCACAACT CTTCTTCATC CCAGAGCCGA 
GGTCAACGCA GCCCGTCTCC CGGACCTTCC TCTTTCTCAG CCGCTTCCTC CTCCTCATCA 
TCGTCCTCCA CAAAGACTGC TGGGAAGCCA GATTCTCCTG GCCTACGCAG ACGGAGAGCT 
TCACCAGTGC CCAGTGGACG TGTGACCCCT CCTCGAAGAG CTCCCTCCCC TGATGGCTTT 
TCCCCATACA GTCCAGAGGA GACCAGCCGC AGGGTCAACA AGGTCATGAG GGCGCGCCTG 
TACCTGCTCC AGCAAATAGG ACCCAACTCA TTCTTGATTG GCGGAGACAG TCCTGACAAC 
AAGTTCAGGG TGTTCATTGG TCCACAGACC TGCAGCTGCG GCAGAGGAAC TTTCTGCATC 
CATGTGCTCT TCGTCATGCT CCGAGTTTTC CAGCTGGAGC CTTCAGATCC TCTTCTGTGG 
CGGAAAACCC TCAAGAATTT TGAGGTGGAG AGCTTATTTC AGAAGTACCA CAATCGCCGC 
AGCTCCCGCA TCAAAGCTCC CTCACGCAGC ACCATCCAGA AGTTTGTGTC CCGCATGTCC 
AACTCTCACA CCTCGTGTAC CTCCAGTGCC TCACCATCCA GTAATGAAAG CAGTATGAAG 
GACGAGGAGG AGCAGATGTG TCCCATCTGT CTGCTGGACA TGCTGGATGA AGAAAGTCTG 
ACAGTGTGTG AAGAGGGCTG CCGAAACAAA CTCCACCATC ACTGCATGTC TATATGGGCC 
GAGGAGTGCA GAAGAAATCA CGATCCTTTA ATCTGCCCAC TGTGTAGAGC CAAATGGAAG 
CCTCATGATT TTTACAGTTA TGAAGCGCCA TCTACTGTGG AAAGCACAAC CAGCCGCTCT 
CAGAGTCAAA CAGCTGCTTC ATCATCATCA TCATCTTCAT CTCTTCATGC TGAGAGCTCC 
AGGACGGGCC AGGATGGAGA CTTCTCTCTT CCTCAATATG GAGTTCAGCA CATCCCTCAG 
ACGTACACGG AGCTCGCCGA ACCCTGGATC AAGGTGTTTG GATTGGAGCT GGTCGGCTGC 
CTCTTCTCTC GTAACTGGAA CATCAGAGAA ATGGCTCTGC GGCGGCTGTC CCATGACGTG 
AGTGGAGCCC TGCTCTTGGC CAACGGCGAG CACTCATTGG CCGGAGCGGG TGCAGGGGCA 
GGGCTGGCCG AGGTATCTGG AGACGTGGTG GTGGAGTCCT GCTGTAGTGT GCTCTCCATG 
GTCTGTGCAG ACCCTGTCTA CAAAGTCTAC GTAGCAGCGC TGAAAACCCT GCGAGCGATG 
CTTGTCTACA CCCCATGCCA CTCTGCTTCA GAGAGGACTC GATTACAGCA GCTTCTGCGG 
CCCGTGGTGG AAACCATACT GGTCAAGTGT GCAGATGCTA ACAGTCGCAC CAGTCAGCTG 
TCCGTGTCCA CTCTGCTGGA GCTGTGCAAA GGTCAAATGG GAGAACTCGC TGTGGGCAGA 
GAAATTCTCA AAGCAGGCTC TATTGGTATT GGTGGAGTTG ACTATGTCCT GAACTGCATC 
TTGGGCTCTA AAACGGAGGC CAATAACTGG CAAGCACTGC TGGGTCGGCT GTGTCTGATT 
GACAGGCTCT TACTGGAGTT TCCTGCTGAA TTCTACCCTC ACATCGTGTC AGGTGGAGAC 
TGCAACCAGG CGCAAAACGT TGTGGAGAGG TACCAGAAGC TGCTGCCTCT TCTAAGTTTT 
GCTCTGAAGT CCATTGATAA CTCTCACTCC ATGGTGGGAA AGCTTTCTCG TCGTGTCTTC 
CTGAGCGCTG CCCGTATGGT AGCTCGTGTG CCTCATGTCT TCGTTAAGCT ACTGGACATG 
CTCAACGTCA CCAGCTCCAC CCACTATGCA CGCATGCGCC GGCGTCTTCT GGCCATTGCG 
GAGGAGATGG ACATCCTGGA TGCGATTCAT CTGGGACTAG ATGACCTACA GAAAGAGCAC 
ACCTCACCCT CTTGTGCTTT CCTTGAACCC TCAGCCCCTC ATAACTCCCC ATGTACGACT 
GAAAGAAGCT CCCCCACCCA TGGTCTACAG CAGCCCGCCG CTAAGGTGGC ACTTTCAGGT 
CAAGCCAGTT CTACTCCTGT AGAGGACATA ACCGAGAGAC TCTCAAACAT AGTCGCTAGC 
CCTGCTGAAT TTCCAAAGCC CCCCATTCAG GCTCGAAACA GACCCGTTAG TCAGTGTCTG 
AACTCCCCCT CATCTGGTCA ACCCCCAAGC CTTCCTTCTC TGTCCATGGC CAATGCCTCA 
GCCTTCCCTC CAGAAAACCC CAAAGCCCTG CCACAGAGTT TTATTCCCAG CAAGTTTGCC 
CAGTCCTCAC TGGGTTCCCA ACGCAAGTTG CCTCTTCAGA AAGGTGCCTG TAGTAGTAAA 
GAACCAGAGA AGCTTCCATC CCCTGTGTTT ACTCAAGCTC GGCCACTGCC CCAGAGTCAG 
ATCCACCGGC CCAGACCTTC TCGACCCACT CCTGCTGCCT CATCTGATGG AGCTAAAGCT 
TTAAGCCCTT TGACAAAGGG CAACATGAAA CTGGACCTTC AGGAGGCCCG GTCCAGAGAT 
GATGTGGCCT GCAGTGGGGG ATCTGCTCTC ATCCCCAGTG AGGACACTGC CTTCACGCCA 
GTGGAGGAGA AGTTCCACGG TCTGGATGCT TCAGCCGATC TCAGCTCCAG CATGGAGGAC 
CTGCTGGAAG CCTCTATCCC TGCGGCAGAC AGCACGGTCA CCTTCCAGTC TGAAGTGGCA 
GTGCTGTCCC CCGAGCAGGC AAATGCAAAC GATGACACGT ACAGTGAAGA CGTGACCCAG 
AACCAGAAGT GCAAGGAGAA GATGGAAGCT GAAGAAGAGG AGGCATTGGC TGTAGTCATG 
GCCATGTCTG AGTCACAAGA CGCCCTACCT GTAATCCCAC AGTTACAGGT GGACAAAGGG 
GAGGACGTGA TCATTATTCA GGTGGATACA CCAGAGACTC TGCCTGGTCA TACCAAAGCA 
AAGCAGCCAT ACAGGGAGGG AAACGAATGG CTAAAAGGTC AACAGATTGG CCTGGGTGCC 
TTTTCGTCAT GTTACCAGGC TCAGGATGTG GGCACTGGGA CTCTGATGGC TGTCAAACAG 
GTGACGTACG TGAGGAACAC TTCATCAGAG CAGGAGGAGG TGGTTGAGGC GCTACGAGAG 
GAGATTCGCA TGATGGGTCT ACTAAACCAC CCACACATCA TCCGCATGCT GGGGGCAACC 
TGTGAGAAGA ACAACTACAA CCTTTTCGTA GAGTGGATGG CAGGTGGCTC TGTATCTCAT 
CTCTTGAATA AGTATGGCGC ATTTAAAGAA GGTGTGATCA TCAACTACAC GGAGCAGCTT 
CTGCGGGGTC TGGCCTACCT TCATGAGAAC CAGATCATCC ACCGCGACAT CAAAGGTGCC 
AATCTACTGA TTGACAGCAC AGGCCAGAGG CTGAGGATAG CTGATTTTGG GGCTGCAGCA 
CGTTTGGCTT CGAAAGGCAC TGGAGCAGGG GAGTTCCAGG GTCAACTTCT GGGAACCATT 
GCCTTCATGG CTCCGGAGGT GCTGAGAGGA CAGCAGTACG GCCGGAGCTG TGATGTCTGG 
AGTGTTGGCT GTGCCATCAT TGAGATGTCC TGTGCTAAAC CTCCCTGGAA CGCTGAAAAA 
CACTCCAACC ACCTGGCGCT CATTTTCAAG ATCGCCAGCG CCACCACAGC GCCCTCCATC 
CCACCACACT TGTCTCCAGG CCTGCGGGAC GTGACCCTGC GCTGTCTGGA GCTCCAGCCC 
ACCGACCGGC CGCCCTCCAG AGAGCTCCTG AAACACCCCG TCTTCAGACA CAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155564.1
CDS	36..4475
Translation	MATAGSRTSSLGGLLDSSSGTNVGNGLQGTSNGSTSQNNSGKVKRNAGESSQWRRKVRSVDLDKCESGTAALHFLTVSGPGSIQTALLQQSLADPPLGDKISNNLCTSAQEWMKSSLKSGSESPSAEHGGAAAAVDVNASQNSRDMENKETLRGLQKMEDRPEERLIREKLKATCMPTWKSEWLERRSRRGPMVVKPIPLRADGTEAGSDSHNSSSSQSRGQRSPSPGPSSFSAASSSSSSSSTKTAGKPDSPGLRRRRASPVPSGRVTPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKFRVFIGPQTCSCGRGTFCIHVLFVMLRVFQLEPSDPLLWRKTLKNFEVESLFQKYHNRRSSRIKAPSRSTIQKFVSRMSNSHTSCTSSASPSSNESSMKDEEEQMCPICLLDMLDEESLTVCEEGCRNKLHHHCMSIWAEECRRNHDPLICPLCRAKWKPHDFYSYEAPSTVESTTSRSQSQTAASSSSSSSSLHAESSRTGQDGDFSLPQYGVQHIPQTYTELAEPWIKVFGLELVGCLFSRNWNIREMALRRLSHDVSGALLLANGEHSLAGAGAGAGLAEVSGDVVVESCCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSASERTRLQQLLRPVVETILVKCADANSRTSQLSVSTLLELCKGQMGELAVGREILKAGSIGIGGVDYVLNCILGSKTEANNWQALLGRLCLIDRLLLEFPAEFYPHIVSGGDCNQAQNVVERYQKLLPLLSFALKSIDNSHSMVGKLSRRVFLSAARMVARVPHVFVKLLDMLNVTSSTHYARMRRRLLAIAEEMDILDAIHLGLDDLQKEHTSPSCAFLEPSAPHNSPCTTERSSPTHGLQQPAAKVALSGQASSTPVEDITERLSNIVASPAEFPKPPIQARNRPVSQCLNSPSSGQPPSLPSLSMANASAFPPENPKALPQSFIPSKFAQSSLGSQRKLPLQKGACSSKEPEKLPSPVFTQARPLPQSQIHRPRPSRPTPAASSDGAKALSPLTKGNMKLDLQEARSRDDVACSGGSALIPSEDTAFTPVEEKFHGLDASADLSSSMEDLLEASIPAADSTVTFQSEVAVLSPEQANANDDTYSEDVTQNQKCKEKMEAEEEEALAVVMAMSESQDALPVIPQLQVDKGEDVIIIQVDTPETLPGHTKAKQPYREGNEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGLLNHPHIIRMLGATCEKNNYNLFVEWMAGGSVSHLLNKYGAFKEGVIINYTEQLLRGLAYLHENQIIHRDIKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMSCAKPPWNAEKHSNHLALIFKIASATTAPSIPPHLSPGLRDVTLRCLELQPTDRPPSRELLKHPVFRHSW

Target ORF information:

RefSeq Version	XM_005155507.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155507.4

ORF Insert Sequence:

ATGGCGACGG CGGGGAGCCG GACGTCGTCG TTAGGCGGAT TATTAGACTC CAGTAGCGGC 
ACAAACGTCG GGAACGGACT TCAAGGCACT TCCAACGGCT CCACGTCTCA GAACAATAGC 
GGGAAAGTGA AGAGAAATGC GGGTGAGAGC TCTCAGTGGA GACGAAAGGT GAGGAGTGTG 
GATCTGGATA AATGCGAGAG CGGGACCGCA GCGCTGCACT TCCTCACGGT GTCCGGTCCC 
GGCTCGATCC AGACCGCTTT ACTGCAGCAG AGCCTCGCCG ACCCGCCGCT CGGTGACAAA 
ATAAGCAATA ATTTGTGTAC AAGTGCTCAG GAATGGATGA AAAGCTCTTT AAAAAGCGGC 
AGTGAAAGTC CGAGCGCTGA ACATGGAGGG GCTGCTGCTG CTGTGGACGT GAACGCTTCG 
CAAAACAGCC GGGACATGGA GAACAAGGAG ACTCTGCGCG GGCTGCAGAA GATGGAGGAC 
CGGCCTGAGG AGCGACTGAT CCGAGAGAAG CTCAAGGCCA CCTGTATGCC CACCTGGAAG 
AGCGAGTGGC TGGAGAGGCG GAGCAGGAGA GGACCAATGG TTGTGAAGCC CATTCCTCTG 
AGAGCCGACG GCACTGAAGC CGGGAGTGAC AGTCACAACT CTTCTTCATC CCAGAGCCGA 
GGTCAACGCA GCCCGTCTCC CGGACCTTCC TCTTTCTCAG CCGCTTCCTC CTCCTCATCA 
TCGTCCTCCA CAAAGACTGC TGGGAAGCCA GATTCTCCTG GCCTACGCAG ACGGAGAGCT 
TCACCAGTGC CCAGTGGACG TGTGACCCCT CCTCGAAGAG CTCCCTCCCC TGATGGCTTT 
TCCCCATACA GTCCAGAGGA GACCAGCCGC AGGGTCAACA AGGTCATGAG GGCGCGCCTG 
TACCTGCTCC AGCAAATAGG ACCCAACTCA TTCTTGATTG GCGGAGACAG TCCTGACAAC 
AAGTTCAGGG TGTTCATTGG TCCACAGACC TGCAGCTGCG GCAGAGGAAC TTTCTGCATC 
CATGTGCTCT TCGTCATGCT CCGAGTTTTC CAGCTGGAGC CTTCAGATCC TCTTCTGTGG 
CGGAAAACCC TCAAGAATTT TGAGGTGGAG AGCTTATTTC AGAAGTACCA CAATCGCCGC 
AGCTCCCGCA TCAAAGCTCC CTCACGCAGC ACCATCCAGA AGTTTGTGTC CCGCATGTCC 
AACTCTCACA CCTCGTGTAC CTCCAGTGCC TCACCATCCA GTAATGAAAG CAGTATGAAG 
GACGAGGAGG AGCAGATGTG TCCCATCTGT CTGCTGGACA TGCTGGATGA AGAAAGTCTG 
ACAGTGTGTG AAGAGGGCTG CCGAAACAAA CTCCACCATC ACTGCATGTC TATATGGGCC 
GAGGAGTGCA GAAGAAATCA CGATCCTTTA ATCTGCCCAC TGTGTAGAGC CAAATGGAAG 
CCTCATGATT TTTACAGTTA TGAAGCGCCA TCTACTGTGG AAAGCACAAC CAGCCGCTCT 
CAGAGTCAAA CAGCTGCTTC ATCATCATCA TCATCTTCAT CTCTTCATGC TGAGAGCTCC 
AGGACGGGCC AGGATGGAGA CTTCTCTCTT CCTCAATATG GAGTTCAGCA CATCCCTCAG 
ACGTACACGG AGCTCGCCGA ACCCTGGATC AAGGTGTTTG GATTGGAGCT GGTCGGCTGC 
CTCTTCTCTC GTAACTGGAA CATCAGAGAA ATGGCTCTGC GGCGGCTGTC CCATGACGTG 
AGTGGAGCCC TGCTCTTGGC CAACGGCGAG CACTCATTGG CCGGAGCGGG TGCAGGGGCA 
GGGCTGGCCG AGGTATCTGG AGACGTGGTG GTGGAGTCCT GCTGTAGTGT GCTCTCCATG 
GTCTGTGCAG ACCCTGTCTA CAAAGTCTAC GTAGCAGCGC TGAAAACCCT GCGAGCGATG 
CTTGTCTACA CCCCATGCCA CTCTGCTTCA GAGAGGACTC GATTACAGCA GCTTCTGCGG 
CCCGTGGTGG AAACCATACT GGTCAAGTGT GCAGATGCTA ACAGTCGCAC CAGTCAGCTG 
TCCGTGTCCA CTCTGCTGGA GCTGTGCAAA GGTCAAATGG GAGAACTCGC TGTGGGCAGA 
GAAATTCTCA AAGCAGGCTC TATTGGTATT GGTGGAGTTG ACTATGTCCT GAACTGCATC 
TTGGGCTCTA AAACGGAGGC CAATAACTGG CAAGCACTGC TGGGTCGGCT GTGTCTGATT 
GACAGGCTCT TACTGGAGTT TCCTGCTGAA TTCTACCCTC ACATCGTGTC AGGTGGAGAC 
TGCAACCAGG CGCAAAACGT TGTGGAGAGG TACCAGAAGC TGCTGCCTCT TCTAAGTTTT 
GCTCTGAAGT CCATTGATAA CTCTCACTCC ATGGTGGGAA AGCTTTCTCG TCGTGTCTTC 
CTGAGCGCTG CCCGTATGGT AGCTCGTGTG CCTCATGTCT TCGTTAAGCT ACTGGACATG 
CTCAACGTCA CCAGCTCCAC CCACTATGCA CGCATGCGCC GGCGTCTTCT GGCCATTGCG 
GAGGAGATGG ACATCCTGGA TGCGATTCAT CTGGGACTAG ATGACCTACA GAAAGAGCAC 
ACCTCACCCT CTTGTGCTTT CCTTGAACCC TCAGCCCCTC ATAACTCCCC ATGTACGACT 
GAAAGAAGCT CCCCCACCCA TGGTCTACAG CAGCCCGCCG CTAAGGTGGC ACTTTCAGGT 
CAAGCCAGTT CTACTCCTGT AGAGGACATA ACCGAGAGAC TCTCAAACAT AGTCGCTAGC 
CCTGCTGAAT TTCCAAAGCC CCCCATTCAG GCTCGAAACA GACCCGTTAG TCAGTGTCTG 
AACTCCCCCT CATCTGGTCA ACCCCCAAGC CTTCCTTCTC TGTCCATGGC CAATGCCTCA 
GCCTTCCCTC CAGAAAACCC CAAAGCCCTG CCACAGAGTT TTATTCCCAG CAAGTTTGCC 
CAGTCCTCAC TGGGTTCCCA ACGCAAGTTG CCTCTTCAGA AAGGTGCCTG TAGTAGTAAA 
GAACCAGAGA AGCTTCCATC CCCTGTGTTT ACTCAAGCTC GGCCACTGCC CCAGAGTCAG 
ATCCACCGGC CCAGACCTTC TCGACCCACT CCTGCTGCCT CATCTGATGG AGCTAAAGCT 
TTAAGCCCTT TGACAAAGGG CAACATGAAA CTGGACCTTC AGGAGGCCCG GTCCAGAGAT 
GATGTGGCCT GCAGTGGGGG ATCTGCTCTC ATCCCCAGTG AGGACACTGC CTTCACGCCA 
GTGGAGGAGA AGTTCCACGG TCTGGATGCT TCAGCCGATC TCAGCTCCAG CATGGAGGAC 
CTGCTGGAAG CCTCTATCCC TGCGGCAGAC AGCACGGTCA CCTTCCAGTC TGAAGTGGCA 
GTGCTGTCCC CCGAGCAGGC AAATGCAAAC GATGACACGT ACAGTGAAGA CGTGACCCAG 
AACCAGAAGT GCAAGGAGAA GATGGAAGCT GAAGAAGAGG AGGCATTGGC TGTAGTCATG 
GCCATGTCTG AGTCACAAGA CGCCCTACCT GTAATCCCAC AGTTACAGGT GGACAAAGGG 
GAGGACGTGA TCATTATTCA GGTGGATACA CCAGAGACTC TGCCTGGTCA TACCAAAGCA 
AAGCAGCCAT ACAGGGAGGG AAACGAATGG CTAAAAGGTC AACAGATTGG CCTGGGTGCC 
TTTTCGTCAT GTTACCAGGC TCAGGATGTG GGCACTGGGA CTCTGATGGC TGTCAAACAG 
GTGACGTACG TGAGGAACAC TTCATCAGAG CAGGAGGAGG TGGTTGAGGC GCTACGAGAG 
GAGATTCGCA TGATGGGTCT ACTAAACCAC CCACACATCA TCCGCATGCT GGGGGCAACC 
TGTGAGAAGA ACAACTACAA CCTTTTCGTA GAGTGGATGG CAGGTGGCTC TGTATCTCAT 
CTCTTGAATA AGTATGGCGC ATTTAAAGAA GGTGTGATCA TCAACTACAC GGAGCAGCTT 
CTGCGGGGTC TGGCCTACCT TCATGAGAAC CAGATCATCC ACCGCGACAT CAAAGGTGCC 
AATCTACTGA TTGACAGCAC AGGCCAGAGG CTGAGGATAG CTGATTTTGG GGCTGCAGCA 
CGTTTGGCTT CGAAAGGCAC TGGAGCAGGG GAGTTCCAGG GTCAACTTCT GGGAACCATT 
GCCTTCATGG CTCCGGAGGT GCTGAGAGGA CAGCAGTACG GCCGGAGCTG TGATGTCTGG 
AGTGTTGGCT GTGCCATCAT TGAGATGTCC TGTGCTAAAC CTCCCTGGAA CGCTGAAAAA 
CACTCCAACC ACCTGGCGCT CATTTTCAAG ATCGCCAGCG CCACCACAGC GCCCTCCATC 
CCACCACACT TGTCTCCAGG CCTGCGGGAC GTGACCCTGC GCTGTCTGGA GCTCCAGCCC 
ACCGACCGGC CGCCCTCCAG AGAGCTCCTG AAACACCCCG TCTTCAGACA CAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_005155507.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155564.1 Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15816
Clone ID Related Accession (Same CDS sequence)	XM_005155507.4 , XM_005155507.3
Accession Version	XM_005155507.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGG CGGGGAGCCG GACGTCGTCG TTAGGCGGAT TATTAGACTC CAGTAGCGGC 
ACAAACGTCG GGAACGGACT TCAAGGCACT TCCAACGGCT CCACGTCTCA GAACAATAGC 
GGGAAAGTGA AGAGAAATGC GGGTGAGAGC TCTCAGTGGA GACGAAAGGT GAGGAGTGTG 
GATCTGGATA AATGCGAGAG CGGGACCGCA GCGCTGCACT TCCTCACGGT GTCCGGTCCC 
GGCTCGATCC AGACCGCTTT ACTGCAGCAG AGCCTCGCCG ACCCGCCGCT CGGTGACAAA 
ATAAGCAATA ATTTGTGTAC AAGTGCTCAG GAATGGATGA AAAGCTCTTT AAAAAGCGGC 
AGTGAAAGTC CGAGCGCTGA ACATGGAGGG GCTGCTGCTG CTGTGGACGT GAACGCTTCG 
CAAAACAGCC GGGACATGGA GAACAAGGAG ACTCTGCGCG GGCTGCAGAA GATGGAGGAC 
CGGCCTGAGG AGCGACTGAT CCGAGAGAAG CTCAAGGCCA CCTGTATGCC CACCTGGAAG 
AGCGAGTGGC TGGAGAGGCG GAGCAGGAGA GGACCAATGG TTGTGAAGCC CATTCCTCTG 
AGAGCCGACG GCACTGAAGC CGGGAGTGAC AGTCACAACT CTTCTTCATC CCAGAGCCGA 
GGTCAACGCA GCCCGTCTCC CGGACCTTCC TCTTTCTCAG CCGCTTCCTC CTCCTCATCA 
TCGTCCTCCA CAAAGACTGC TGGGAAGCCA GATTCTCCTG GCCTACGCAG ACGGAGAGCT 
TCACCAGTGC CCAGTGGACG TGTGACCCCT CCTCGAAGAG CTCCCTCCCC TGATGGCTTT 
TCCCCATACA GTCCAGAGGA GACCAGCCGC AGGGTCAACA AGGTCATGAG GGCGCGCCTG 
TACCTGCTCC AGCAAATAGG ACCCAACTCA TTCTTGATTG GCGGAGACAG TCCTGACAAC 
AAGTTCAGGG TGTTCATTGG TCCACAGACC TGCAGCTGCG GCAGAGGAAC TTTCTGCATC 
CATGTGCTCT TCGTCATGCT CCGAGTTTTC CAGCTGGAGC CTTCAGATCC TCTTCTGTGG 
CGGAAAACCC TCAAGAATTT TGAGGTGGAG AGCTTATTTC AGAAGTACCA CAATCGCCGC 
AGCTCCCGCA TCAAAGCTCC CTCACGCAGC ACCATCCAGA AGTTTGTGTC CCGCATGTCC 
AACTCTCACA CCTCGTGTAC CTCCAGTGCC TCACCATCCA GTAATGAAAG CAGTATGAAG 
GACGAGGAGG AGCAGATGTG TCCCATCTGT CTGCTGGACA TGCTGGATGA AGAAAGTCTG 
ACAGTGTGTG AAGAGGGCTG CCGAAACAAA CTCCACCATC ACTGCATGTC TATATGGGCC 
GAGGAGTGCA GAAGAAATCA CGATCCTTTA ATCTGCCCAC TGTGTAGAGC CAAATGGAAG 
CCTCATGATT TTTACAGTTA TGAAGCGCCA TCTACTGTGG AAAGCACAAC CAGCCGCTCT 
CAGAGTCAAA CAGCTGCTTC ATCATCATCA TCATCTTCAT CTCTTCATGC TGAGAGCTCC 
AGGACGGGCC AGGATGGAGA CTTCTCTCTT CCTCAATATG GAGTTCAGCA CATCCCTCAG 
ACGTACACGG AGCTCGCCGA ACCCTGGATC AAGGTGTTTG GATTGGAGCT GGTCGGCTGC 
CTCTTCTCTC GTAACTGGAA CATCAGAGAA ATGGCTCTGC GGCGGCTGTC CCATGACGTG 
AGTGGAGCCC TGCTCTTGGC CAACGGCGAG CACTCATTGG CCGGAGCGGG TGCAGGGGCA 
GGGCTGGCCG AGGTATCTGG AGACGTGGTG GTGGAGTCCT GCTGTAGTGT GCTCTCCATG 
GTCTGTGCAG ACCCTGTCTA CAAAGTCTAC GTAGCAGCGC TGAAAACCCT GCGAGCGATG 
CTTGTCTACA CCCCATGCCA CTCTGCTTCA GAGAGGACTC GATTACAGCA GCTTCTGCGG 
CCCGTGGTGG AAACCATACT GGTCAAGTGT GCAGATGCTA ACAGTCGCAC CAGTCAGCTG 
TCCGTGTCCA CTCTGCTGGA GCTGTGCAAA GGTCAAATGG GAGAACTCGC TGTGGGCAGA 
GAAATTCTCA AAGCAGGCTC TATTGGTATT GGTGGAGTTG ACTATGTCCT GAACTGCATC 
TTGGGCTCTA AAACGGAGGC CAATAACTGG CAAGCACTGC TGGGTCGGCT GTGTCTGATT 
GACAGGCTCT TACTGGAGTT TCCTGCTGAA TTCTACCCTC ACATCGTGTC AGGTGGAGAC 
TGCAACCAGG CGCAAAACGT TGTGGAGAGG TACCAGAAGC TGCTGCCTCT TCTAAGTTTT 
GCTCTGAAGT CCATTGATAA CTCTCACTCC ATGGTGGGAA AGCTTTCTCG TCGTGTCTTC 
CTGAGCGCTG CCCGTATGGT AGCTCGTGTG CCTCATGTCT TCGTTAAGCT ACTGGACATG 
CTCAACGTCA CCAGCTCCAC CCACTATGCA CGCATGCGCC GGCGTCTTCT GGCCATTGCG 
GAGGAGATGG ACATCCTGGA TGCGATTCAT CTGGGACTAG ATGACCTACA GAAAGAGCAC 
ACCTCACCCT CTTGTGCTTT CCTTGAACCC TCAGCCCCTC ATAACTCCCC ATGTACGACT 
GAAAGAAGCT CCCCCACCCA TGGTCTACAG CAGCCCGCCG CTAAGGTGGC ACTTTCAGGT 
CAAGCCAGTT CTACTCCTGT AGAGGACATA ACCGAGAGAC TCTCAAACAT AGTCGCTAGC 
CCTGCTGAAT TTCCAAAGCC CCCCATTCAG GCTCGAAACA GACCCGTTAG TCAGTGTCTG 
AACTCCCCCT CATCTGGTCA ACCCCCAAGC CTTCCTTCTC TGTCCATGGC CAATGCCTCA 
GCCTTCCCTC CAGAAAACCC CAAAGCCCTG CCACAGAGTT TTATTCCCAG CAAGTTTGCC 
CAGTCCTCAC TGGGTTCCCA ACGCAAGTTG CCTCTTCAGA AAGGTGCCTG TAGTAGTAAA 
GAACCAGAGA AGCTTCCATC CCCTGTGTTT ACTCAAGCTC GGCCACTGCC CCAGAGTCAG 
ATCCACCGGC CCAGACCTTC TCGACCCACT CCTGCTGCCT CATCTGATGG AGCTAAAGCT 
TTAAGCCCTT TGACAAAGGG CAACATGAAA CTGGACCTTC AGGAGGCCCG GTCCAGAGAT 
GATGTGGCCT GCAGTGGGGG ATCTGCTCTC ATCCCCAGTG AGGACACTGC CTTCACGCCA 
GTGGAGGAGA AGTTCCACGG TCTGGATGCT TCAGCCGATC TCAGCTCCAG CATGGAGGAC 
CTGCTGGAAG CCTCTATCCC TGCGGCAGAC AGCACGGTCA CCTTCCAGTC TGAAGTGGCA 
GTGCTGTCCC CCGAGCAGGC AAATGCAAAC GATGACACGT ACAGTGAAGA CGTGACCCAG 
AACCAGAAGT GCAAGGAGAA GATGGAAGCT GAAGAAGAGG AGGCATTGGC TGTAGTCATG 
GCCATGTCTG AGTCACAAGA CGCCCTACCT GTAATCCCAC AGTTACAGGT GGACAAAGGG 
GAGGACGTGA TCATTATTCA GGTGGATACA CCAGAGACTC TGCCTGGTCA TACCAAAGCA 
AAGCAGCCAT ACAGGGAGGG AAACGAATGG CTAAAAGGTC AACAGATTGG CCTGGGTGCC 
TTTTCGTCAT GTTACCAGGC TCAGGATGTG GGCACTGGGA CTCTGATGGC TGTCAAACAG 
GTGACGTACG TGAGGAACAC TTCATCAGAG CAGGAGGAGG TGGTTGAGGC GCTACGAGAG 
GAGATTCGCA TGATGGGTCT ACTAAACCAC CCACACATCA TCCGCATGCT GGGGGCAACC 
TGTGAGAAGA ACAACTACAA CCTTTTCGTA GAGTGGATGG CAGGTGGCTC TGTATCTCAT 
CTCTTGAATA AGTATGGCGC ATTTAAAGAA GGTGTGATCA TCAACTACAC GGAGCAGCTT 
CTGCGGGGTC TGGCCTACCT TCATGAGAAC CAGATCATCC ACCGCGACAT CAAAGGTGCC 
AATCTACTGA TTGACAGCAC AGGCCAGAGG CTGAGGATAG CTGATTTTGG GGCTGCAGCA 
CGTTTGGCTT CGAAAGGCAC TGGAGCAGGG GAGTTCCAGG GTCAACTTCT GGGAACCATT 
GCCTTCATGG CTCCGGAGGT GCTGAGAGGA CAGCAGTACG GCCGGAGCTG TGATGTCTGG 
AGTGTTGGCT GTGCCATCAT TGAGATGTCC TGTGCTAAAC CTCCCTGGAA CGCTGAAAAA 
CACTCCAACC ACCTGGCGCT CATTTTCAAG ATCGCCAGCG CCACCACAGC GCCCTCCATC 
CCACCACACT TGTCTCCAGG CCTGCGGGAC GTGACCCTGC GCTGTCTGGA GCTCCAGCCC 
ACCGACCGGC CGCCCTCCAG AGAGCTCCTG AAACACCCCG TCTTCAGACA CAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155564.1
CDS	39..4478
Translation	MATAGSRTSSLGGLLDSSSGTNVGNGLQGTSNGSTSQNNSGKVKRNAGESSQWRRKVRSVDLDKCESGTAALHFLTVSGPGSIQTALLQQSLADPPLGDKISNNLCTSAQEWMKSSLKSGSESPSAEHGGAAAAVDVNASQNSRDMENKETLRGLQKMEDRPEERLIREKLKATCMPTWKSEWLERRSRRGPMVVKPIPLRADGTEAGSDSHNSSSSQSRGQRSPSPGPSSFSAASSSSSSSSTKTAGKPDSPGLRRRRASPVPSGRVTPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKFRVFIGPQTCSCGRGTFCIHVLFVMLRVFQLEPSDPLLWRKTLKNFEVESLFQKYHNRRSSRIKAPSRSTIQKFVSRMSNSHTSCTSSASPSSNESSMKDEEEQMCPICLLDMLDEESLTVCEEGCRNKLHHHCMSIWAEECRRNHDPLICPLCRAKWKPHDFYSYEAPSTVESTTSRSQSQTAASSSSSSSSLHAESSRTGQDGDFSLPQYGVQHIPQTYTELAEPWIKVFGLELVGCLFSRNWNIREMALRRLSHDVSGALLLANGEHSLAGAGAGAGLAEVSGDVVVESCCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSASERTRLQQLLRPVVETILVKCADANSRTSQLSVSTLLELCKGQMGELAVGREILKAGSIGIGGVDYVLNCILGSKTEANNWQALLGRLCLIDRLLLEFPAEFYPHIVSGGDCNQAQNVVERYQKLLPLLSFALKSIDNSHSMVGKLSRRVFLSAARMVARVPHVFVKLLDMLNVTSSTHYARMRRRLLAIAEEMDILDAIHLGLDDLQKEHTSPSCAFLEPSAPHNSPCTTERSSPTHGLQQPAAKVALSGQASSTPVEDITERLSNIVASPAEFPKPPIQARNRPVSQCLNSPSSGQPPSLPSLSMANASAFPPENPKALPQSFIPSKFAQSSLGSQRKLPLQKGACSSKEPEKLPSPVFTQARPLPQSQIHRPRPSRPTPAASSDGAKALSPLTKGNMKLDLQEARSRDDVACSGGSALIPSEDTAFTPVEEKFHGLDASADLSSSMEDLLEASIPAADSTVTFQSEVAVLSPEQANANDDTYSEDVTQNQKCKEKMEAEEEEALAVVMAMSESQDALPVIPQLQVDKGEDVIIIQVDTPETLPGHTKAKQPYREGNEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGLLNHPHIIRMLGATCEKNNYNLFVEWMAGGSVSHLLNKYGAFKEGVIINYTEQLLRGLAYLHENQIIHRDIKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMSCAKPPWNAEKHSNHLALIFKIASATTAPSIPPHLSPGLRDVTLRCLELQPTDRPPSRELLKHPVFRHSW

Target ORF information:

RefSeq Version	XM_005155507.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155507.3

ORF Insert Sequence:

ATGGCGACGG CGGGGAGCCG GACGTCGTCG TTAGGCGGAT TATTAGACTC CAGTAGCGGC 
ACAAACGTCG GGAACGGACT TCAAGGCACT TCCAACGGCT CCACGTCTCA GAACAATAGC 
GGGAAAGTGA AGAGAAATGC GGGTGAGAGC TCTCAGTGGA GACGAAAGGT GAGGAGTGTG 
GATCTGGATA AATGCGAGAG CGGGACCGCA GCGCTGCACT TCCTCACGGT GTCCGGTCCC 
GGCTCGATCC AGACCGCTTT ACTGCAGCAG AGCCTCGCCG ACCCGCCGCT CGGTGACAAA 
ATAAGCAATA ATTTGTGTAC AAGTGCTCAG GAATGGATGA AAAGCTCTTT AAAAAGCGGC 
AGTGAAAGTC CGAGCGCTGA ACATGGAGGG GCTGCTGCTG CTGTGGACGT GAACGCTTCG 
CAAAACAGCC GGGACATGGA GAACAAGGAG ACTCTGCGCG GGCTGCAGAA GATGGAGGAC 
CGGCCTGAGG AGCGACTGAT CCGAGAGAAG CTCAAGGCCA CCTGTATGCC CACCTGGAAG 
AGCGAGTGGC TGGAGAGGCG GAGCAGGAGA GGACCAATGG TTGTGAAGCC CATTCCTCTG 
AGAGCCGACG GCACTGAAGC CGGGAGTGAC AGTCACAACT CTTCTTCATC CCAGAGCCGA 
GGTCAACGCA GCCCGTCTCC CGGACCTTCC TCTTTCTCAG CCGCTTCCTC CTCCTCATCA 
TCGTCCTCCA CAAAGACTGC TGGGAAGCCA GATTCTCCTG GCCTACGCAG ACGGAGAGCT 
TCACCAGTGC CCAGTGGACG TGTGACCCCT CCTCGAAGAG CTCCCTCCCC TGATGGCTTT 
TCCCCATACA GTCCAGAGGA GACCAGCCGC AGGGTCAACA AGGTCATGAG GGCGCGCCTG 
TACCTGCTCC AGCAAATAGG ACCCAACTCA TTCTTGATTG GCGGAGACAG TCCTGACAAC 
AAGTTCAGGG TGTTCATTGG TCCACAGACC TGCAGCTGCG GCAGAGGAAC TTTCTGCATC 
CATGTGCTCT TCGTCATGCT CCGAGTTTTC CAGCTGGAGC CTTCAGATCC TCTTCTGTGG 
CGGAAAACCC TCAAGAATTT TGAGGTGGAG AGCTTATTTC AGAAGTACCA CAATCGCCGC 
AGCTCCCGCA TCAAAGCTCC CTCACGCAGC ACCATCCAGA AGTTTGTGTC CCGCATGTCC 
AACTCTCACA CCTCGTGTAC CTCCAGTGCC TCACCATCCA GTAATGAAAG CAGTATGAAG 
GACGAGGAGG AGCAGATGTG TCCCATCTGT CTGCTGGACA TGCTGGATGA AGAAAGTCTG 
ACAGTGTGTG AAGAGGGCTG CCGAAACAAA CTCCACCATC ACTGCATGTC TATATGGGCC 
GAGGAGTGCA GAAGAAATCA CGATCCTTTA ATCTGCCCAC TGTGTAGAGC CAAATGGAAG 
CCTCATGATT TTTACAGTTA TGAAGCGCCA TCTACTGTGG AAAGCACAAC CAGCCGCTCT 
CAGAGTCAAA CAGCTGCTTC ATCATCATCA TCATCTTCAT CTCTTCATGC TGAGAGCTCC 
AGGACGGGCC AGGATGGAGA CTTCTCTCTT CCTCAATATG GAGTTCAGCA CATCCCTCAG 
ACGTACACGG AGCTCGCCGA ACCCTGGATC AAGGTGTTTG GATTGGAGCT GGTCGGCTGC 
CTCTTCTCTC GTAACTGGAA CATCAGAGAA ATGGCTCTGC GGCGGCTGTC CCATGACGTG 
AGTGGAGCCC TGCTCTTGGC CAACGGCGAG CACTCATTGG CCGGAGCGGG TGCAGGGGCA 
GGGCTGGCCG AGGTATCTGG AGACGTGGTG GTGGAGTCCT GCTGTAGTGT GCTCTCCATG 
GTCTGTGCAG ACCCTGTCTA CAAAGTCTAC GTAGCAGCGC TGAAAACCCT GCGAGCGATG 
CTTGTCTACA CCCCATGCCA CTCTGCTTCA GAGAGGACTC GATTACAGCA GCTTCTGCGG 
CCCGTGGTGG AAACCATACT GGTCAAGTGT GCAGATGCTA ACAGTCGCAC CAGTCAGCTG 
TCCGTGTCCA CTCTGCTGGA GCTGTGCAAA GGTCAAATGG GAGAACTCGC TGTGGGCAGA 
GAAATTCTCA AAGCAGGCTC TATTGGTATT GGTGGAGTTG ACTATGTCCT GAACTGCATC 
TTGGGCTCTA AAACGGAGGC CAATAACTGG CAAGCACTGC TGGGTCGGCT GTGTCTGATT 
GACAGGCTCT TACTGGAGTT TCCTGCTGAA TTCTACCCTC ACATCGTGTC AGGTGGAGAC 
TGCAACCAGG CGCAAAACGT TGTGGAGAGG TACCAGAAGC TGCTGCCTCT TCTAAGTTTT 
GCTCTGAAGT CCATTGATAA CTCTCACTCC ATGGTGGGAA AGCTTTCTCG TCGTGTCTTC 
CTGAGCGCTG CCCGTATGGT AGCTCGTGTG CCTCATGTCT TCGTTAAGCT ACTGGACATG 
CTCAACGTCA CCAGCTCCAC CCACTATGCA CGCATGCGCC GGCGTCTTCT GGCCATTGCG 
GAGGAGATGG ACATCCTGGA TGCGATTCAT CTGGGACTAG ATGACCTACA GAAAGAGCAC 
ACCTCACCCT CTTGTGCTTT CCTTGAACCC TCAGCCCCTC ATAACTCCCC ATGTACGACT 
GAAAGAAGCT CCCCCACCCA TGGTCTACAG CAGCCCGCCG CTAAGGTGGC ACTTTCAGGT 
CAAGCCAGTT CTACTCCTGT AGAGGACATA ACCGAGAGAC TCTCAAACAT AGTCGCTAGC 
CCTGCTGAAT TTCCAAAGCC CCCCATTCAG GCTCGAAACA GACCCGTTAG TCAGTGTCTG 
AACTCCCCCT CATCTGGTCA ACCCCCAAGC CTTCCTTCTC TGTCCATGGC CAATGCCTCA 
GCCTTCCCTC CAGAAAACCC CAAAGCCCTG CCACAGAGTT TTATTCCCAG CAAGTTTGCC 
CAGTCCTCAC TGGGTTCCCA ACGCAAGTTG CCTCTTCAGA AAGGTGCCTG TAGTAGTAAA 
GAACCAGAGA AGCTTCCATC CCCTGTGTTT ACTCAAGCTC GGCCACTGCC CCAGAGTCAG 
ATCCACCGGC CCAGACCTTC TCGACCCACT CCTGCTGCCT CATCTGATGG AGCTAAAGCT 
TTAAGCCCTT TGACAAAGGG CAACATGAAA CTGGACCTTC AGGAGGCCCG GTCCAGAGAT 
GATGTGGCCT GCAGTGGGGG ATCTGCTCTC ATCCCCAGTG AGGACACTGC CTTCACGCCA 
GTGGAGGAGA AGTTCCACGG TCTGGATGCT TCAGCCGATC TCAGCTCCAG CATGGAGGAC 
CTGCTGGAAG CCTCTATCCC TGCGGCAGAC AGCACGGTCA CCTTCCAGTC TGAAGTGGCA 
GTGCTGTCCC CCGAGCAGGC AAATGCAAAC GATGACACGT ACAGTGAAGA CGTGACCCAG 
AACCAGAAGT GCAAGGAGAA GATGGAAGCT GAAGAAGAGG AGGCATTGGC TGTAGTCATG 
GCCATGTCTG AGTCACAAGA CGCCCTACCT GTAATCCCAC AGTTACAGGT GGACAAAGGG 
GAGGACGTGA TCATTATTCA GGTGGATACA CCAGAGACTC TGCCTGGTCA TACCAAAGCA 
AAGCAGCCAT ACAGGGAGGG AAACGAATGG CTAAAAGGTC AACAGATTGG CCTGGGTGCC 
TTTTCGTCAT GTTACCAGGC TCAGGATGTG GGCACTGGGA CTCTGATGGC TGTCAAACAG 
GTGACGTACG TGAGGAACAC TTCATCAGAG CAGGAGGAGG TGGTTGAGGC GCTACGAGAG 
GAGATTCGCA TGATGGGTCT ACTAAACCAC CCACACATCA TCCGCATGCT GGGGGCAACC 
TGTGAGAAGA ACAACTACAA CCTTTTCGTA GAGTGGATGG CAGGTGGCTC TGTATCTCAT 
CTCTTGAATA AGTATGGCGC ATTTAAAGAA GGTGTGATCA TCAACTACAC GGAGCAGCTT 
CTGCGGGGTC TGGCCTACCT TCATGAGAAC CAGATCATCC ACCGCGACAT CAAAGGTGCC 
AATCTACTGA TTGACAGCAC AGGCCAGAGG CTGAGGATAG CTGATTTTGG GGCTGCAGCA 
CGTTTGGCTT CGAAAGGCAC TGGAGCAGGG GAGTTCCAGG GTCAACTTCT GGGAACCATT 
GCCTTCATGG CTCCGGAGGT GCTGAGAGGA CAGCAGTACG GCCGGAGCTG TGATGTCTGG 
AGTGTTGGCT GTGCCATCAT TGAGATGTCC TGTGCTAAAC CTCCCTGGAA CGCTGAAAAA 
CACTCCAACC ACCTGGCGCT CATTTTCAAG ATCGCCAGCG CCACCACAGC GCCCTCCATC 
CCACCACACT TGTCTCCAGG CCTGCGGGAC GTGACCCTGC GCTGTCTGGA GCTCCAGCCC 
ACCGACCGGC CGCCCTCCAG AGAGCTCCTG AAACACCCCG TCTTCAGACA CAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_009305108.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303383.1 Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X2, mRNA.

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CloneID	ODa49967
Clone ID Related Accession (Same CDS sequence)	XM_009305108.2
Accession Version	XM_009305108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACA AGGAGACTCT GCGCGGGCTG CAGAAGATGG AGGACCGGCC TGAGGAGCGA 
CTGATCCGAG AGAAGCTCAA GGCCACCTGT ATGCCCACCT GGAAGAGCGA GTGGCTGGAG 
AGGCGGAGCA GGAGAGGACC AATGGTTGTG AAGCCCATTC CTCTGAGAGC CGACGGCACT 
GAAGCCGGGA GTGACAGTCA CAACTCTTCT TCATCCCAGA GCCGAGGTCA ACGCAGCCCG 
TCTCCCGGAC CTTCCTCTTT CTCAGCCGCT TCCTCCTCCT CATCATCGTC CTCCACAAAG 
ACTGCTGGGA AGCCAGATTC TCCTGGCCTA CGCAGACGGA GAGCTTCACC AGTGCCCAGT 
GGACGTGTGA CCCCTCCTCG AAGAGCTCCC TCCCCTGATG GCTTTTCCCC ATACAGTCCA 
GAGGAGACCA GCCGCAGGGT CAACAAGGTC ATGAGGGCGC GCCTGTACCT GCTCCAGCAA 
ATAGGACCCA ACTCATTCTT GATTGGCGGA GACAGTCCTG ACAACAAGTT CAGGGTGTTC 
ATTGGTCCAC AGACCTGCAG CTGCGGCAGA GGAACTTTCT GCATCCATGT GCTCTTCGTC 
ATGCTCCGAG TTTTCCAGCT GGAGCCTTCA GATCCTCTTC TGTGGCGGAA AACCCTCAAG 
AATTTTGAGG TGGAGAGCTT ATTTCAGAAG TACCACAATC GCCGCAGCTC CCGCATCAAA 
GCTCCCTCAC GCAGCACCAT CCAGAAGTTT GTGTCCCGCA TGTCCAACTC TCACACCTCG 
TGTACCTCCA GTGCCTCACC ATCCAGTAAT GAAAGCAGTA TGAAGGACGA GGAGGAGCAG 
ATGTGTCCCA TCTGTCTGCT GGACATGCTG GATGAAGAAA GTCTGACAGT GTGTGAAGAG 
GGCTGCCGAA ACAAACTCCA CCATCACTGC ATGTCTATAT GGGCCGAGGA GTGCAGAAGA 
AATCACGATC CTTTAATCTG CCCACTGTGT AGAGCCAAAT GGAAGCCTCA TGATTTTTAC 
AGTTATGAAG CGCCATCTAC TGTGGAAAGC ACAACCAGCC GCTCTCAGAG TCAAACAGCT 
GCTTCATCAT CATCATCATC TTCATCTCTT CATGCTGAGA GCTCCAGGAC GGGCCAGGAT 
GGAGACTTCT CTCTTCCTCA ATATGGAGTT CAGCACATCC CTCAGACGTA CACGGAGCTC 
GCCGAACCCT GGATCAAGGT GTTTGGATTG GAGCTGGTCG GCTGCCTCTT CTCTCGTAAC 
TGGAACATCA GAGAAATGGC TCTGCGGCGG CTGTCCCATG ACGTGAGTGG AGCCCTGCTC 
TTGGCCAACG GCGAGCACTC ATTGGCCGGA GCGGGTGCAG GGGCAGGGCT GGCCGAGGTA 
TCTGGAGACG TGGTGGTGGA GTCCTGCTGT AGTGTGCTCT CCATGGTCTG TGCAGACCCT 
GTCTACAAAG TCTACGTAGC AGCGCTGAAA ACCCTGCGAG CGATGCTTGT CTACACCCCA 
TGCCACTCTG CTTCAGAGAG GACTCGATTA CAGCAGCTTC TGCGGCCCGT GGTGGAAACC 
ATACTGGTCA AGTGTGCAGA TGCTAACAGT CGCACCAGTC AGCTGTCCGT GTCCACTCTG 
CTGGAGCTGT GCAAAGGTCA AATGGGAGAA CTCGCTGTGG GCAGAGAAAT TCTCAAAGCA 
GGCTCTATTG GTATTGGTGG AGTTGACTAT GTCCTGAACT GCATCTTGGG CTCTAAAACG 
GAGGCCAATA ACTGGCAAGC ACTGCTGGGT CGGCTGTGTC TGATTGACAG GCTCTTACTG 
GAGTTTCCTG CTGAATTCTA CCCTCACATC GTGTCAGGTG GAGACTGCAA CCAGGCGCAA 
AACGTTGTGG AGAGGTACCA GAAGCTGCTG CCTCTTCTAA GTTTTGCTCT GAAGTCCATT 
GATAACTCTC ACTCCATGGT GGGAAAGCTT TCTCGTCGTG TCTTCCTGAG CGCTGCCCGT 
ATGGTAGCTC GTGTGCCTCA TGTCTTCGTT AAGCTACTGG ACATGCTCAA CGTCACCAGC 
TCCACCCACT ATGCACGCAT GCGCCGGCGT CTTCTGGCCA TTGCGGAGGA GATGGACATC 
CTGGATGCGA TTCATCTGGG ACTAGATGAC CTACAGAAAG AGCACACCTC ACCCTCTTGT 
GCTTTCCTTG AACCCTCAGC CCCTCATAAC TCCCCATGTA CGACTGAAAG AAGCTCCCCC 
ACCCATGGTC TACAGCAGCC CGCCGCTAAG GTGGCACTTT CAGGTCAAGC CAGTTCTACT 
CCTGTAGAGG ACATAACCGA GAGACTCTCA AACATAGTCG CTAGCCCTGC TGAATTTCCA 
AAGCCCCCCA TTCAGGCTCG AAACAGACCC GTTAGTCAGT GTCTGAACTC CCCCTCATCT 
GGTCAACCCC CAAGCCTTCC TTCTCTGTCC ATGGCCAATG CCTCAGCCTT CCCTCCAGAA 
AACCCCAAAG CCCTGCCACA GAGTTTTATT CCCAGCAAGT TTGCCCAGTC CTCACTGGGT 
TCCCAACGCA AGTTGCCTCT TCAGAAAGGT GCCTGTAGTA GTAAAGAACC AGAGAAGCTT 
CCATCCCCTG TGTTTACTCA AGCTCGGCCA CTGCCCCAGA GTCAGATCCA CCGGCCCAGA 
CCTTCTCGAC CCACTCCTGC TGCCTCATCT GATGGAGCTA AAGCTTTAAG CCCTTTGACA 
AAGGGCAACA TGAAACTGGA CCTTCAGGAG GCCCGGTCCA GAGATGATGT GGCCTGCAGT 
GGGGGATCTG CTCTCATCCC CAGTGAGGAC ACTGCCTTCA CGCCAGTGGA GGAGAAGTTC 
CACGGTCTGG ATGCTTCAGC CGATCTCAGC TCCAGCATGG AGGACCTGCT GGAAGCCTCT 
ATCCCTGCGG CAGACAGCAC GGTCACCTTC CAGTCTGAAG TGGCAGTGCT GTCCCCCGAG 
CAGGCAAATG CAAACGATGA CACGTACAGT GAAGACGTGA CCCAGAACCA GAAGTGCAAG 
GAGAAGATGG AAGCTGAAGA AGAGGAGGCA TTGGCTGTAG TCATGGCCAT GTCTGAGTCA 
CAAGACGCCC TACCTGTAAT CCCACAGTTA CAGGTGGACA AAGGGGAGGA CGTGATCATT 
ATTCAGGTGG ATACACCAGA GACTCTGCCT GGTCATACCA AAGCAAAGCA GCCATACAGG 
GAGGGAAACG AATGGCTAAA AGGTCAACAG ATTGGCCTGG GTGCCTTTTC GTCATGTTAC 
CAGGCTCAGG ATGTGGGCAC TGGGACTCTG ATGGCTGTCA AACAGGTGAC GTACGTGAGG 
AACACTTCAT CAGAGCAGGA GGAGGTGGTT GAGGCGCTAC GAGAGGAGAT TCGCATGATG 
GGTCTACTAA ACCACCCACA CATCATCCGC ATGCTGGGGG CAACCTGTGA GAAGAACAAC 
TACAACCTTT TCGTAGAGTG GATGGCAGGT GGCTCTGTAT CTCATCTCTT GAATAAGTAT 
GGCGCATTTA AAGAAGGTGT GATCATCAAC TACACGGAGC AGCTTCTGCG GGGTCTGGCC 
TACCTTCATG AGAACCAGAT CATCCACCGC GACATCAAAG GTGCCAATCT ACTGATTGAC 
AGCACAGGCC AGAGGCTGAG GATAGCTGAT TTTGGGGCTG CAGCACGTTT GGCTTCGAAA 
GGCACTGGAG CAGGGGAGTT CCAGGGTCAA CTTCTGGGAA CCATTGCCTT CATGGCTCCG 
GAGGTGCTGA GAGGACAGCA GTACGGCCGG AGCTGTGATG TCTGGAGTGT TGGCTGTGCC 
ATCATTGAGA TGTCCTGTGC TAAACCTCCC TGGAACGCTG AAAAACACTC CAACCACCTG 
GCGCTCATTT TCAAGATCGC CAGCGCCACC ACAGCGCCCT CCATCCCACC ACACTTGTCT 
CCAGGCCTGC GGGACGTGAC CCTGCGCTGT CTGGAGCTCC AGCCCACCGA CCGGCCGCCC 
TCCAGAGAGC TCCTGAAACA CCCCGTCTTC AGACACAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303383.1
CDS	108..4112
Translation	MENKETLRGLQKMEDRPEERLIREKLKATCMPTWKSEWLERRSRRGPMVVKPIPLRADGTEAGSDSHNSSSSQSRGQRSPSPGPSSFSAASSSSSSSSTKTAGKPDSPGLRRRRASPVPSGRVTPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKFRVFIGPQTCSCGRGTFCIHVLFVMLRVFQLEPSDPLLWRKTLKNFEVESLFQKYHNRRSSRIKAPSRSTIQKFVSRMSNSHTSCTSSASPSSNESSMKDEEEQMCPICLLDMLDEESLTVCEEGCRNKLHHHCMSIWAEECRRNHDPLICPLCRAKWKPHDFYSYEAPSTVESTTSRSQSQTAASSSSSSSSLHAESSRTGQDGDFSLPQYGVQHIPQTYTELAEPWIKVFGLELVGCLFSRNWNIREMALRRLSHDVSGALLLANGEHSLAGAGAGAGLAEVSGDVVVESCCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSASERTRLQQLLRPVVETILVKCADANSRTSQLSVSTLLELCKGQMGELAVGREILKAGSIGIGGVDYVLNCILGSKTEANNWQALLGRLCLIDRLLLEFPAEFYPHIVSGGDCNQAQNVVERYQKLLPLLSFALKSIDNSHSMVGKLSRRVFLSAARMVARVPHVFVKLLDMLNVTSSTHYARMRRRLLAIAEEMDILDAIHLGLDDLQKEHTSPSCAFLEPSAPHNSPCTTERSSPTHGLQQPAAKVALSGQASSTPVEDITERLSNIVASPAEFPKPPIQARNRPVSQCLNSPSSGQPPSLPSLSMANASAFPPENPKALPQSFIPSKFAQSSLGSQRKLPLQKGACSSKEPEKLPSPVFTQARPLPQSQIHRPRPSRPTPAASSDGAKALSPLTKGNMKLDLQEARSRDDVACSGGSALIPSEDTAFTPVEEKFHGLDASADLSSSMEDLLEASIPAADSTVTFQSEVAVLSPEQANANDDTYSEDVTQNQKCKEKMEAEEEEALAVVMAMSESQDALPVIPQLQVDKGEDVIIIQVDTPETLPGHTKAKQPYREGNEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGLLNHPHIIRMLGATCEKNNYNLFVEWMAGGSVSHLLNKYGAFKEGVIINYTEQLLRGLAYLHENQIIHRDIKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMSCAKPPWNAEKHSNHLALIFKIASATTAPSIPPHLSPGLRDVTLRCLELQPTDRPPSRELLKHPVFRHSW

Target ORF information:

RefSeq Version	XM_009305108.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305108.2

ORF Insert Sequence:

ATGGAGAACA AGGAGACTCT GCGCGGGCTG CAGAAGATGG AGGACCGGCC TGAGGAGCGA 
CTGATCCGAG AGAAGCTCAA GGCCACCTGT ATGCCCACCT GGAAGAGCGA GTGGCTGGAG 
AGGCGGAGCA GGAGAGGACC AATGGTTGTG AAGCCCATTC CTCTGAGAGC CGACGGCACT 
GAAGCCGGGA GTGACAGTCA CAACTCTTCT TCATCCCAGA GCCGAGGTCA ACGCAGCCCG 
TCTCCCGGAC CTTCCTCTTT CTCAGCCGCT TCCTCCTCCT CATCATCGTC CTCCACAAAG 
ACTGCTGGGA AGCCAGATTC TCCTGGCCTA CGCAGACGGA GAGCTTCACC AGTGCCCAGT 
GGACGTGTGA CCCCTCCTCG AAGAGCTCCC TCCCCTGATG GCTTTTCCCC ATACAGTCCA 
GAGGAGACCA GCCGCAGGGT CAACAAGGTC ATGAGGGCGC GCCTGTACCT GCTCCAGCAA 
ATAGGACCCA ACTCATTCTT GATTGGCGGA GACAGTCCTG ACAACAAGTT CAGGGTGTTC 
ATTGGTCCAC AGACCTGCAG CTGCGGCAGA GGAACTTTCT GCATCCATGT GCTCTTCGTC 
ATGCTCCGAG TTTTCCAGCT GGAGCCTTCA GATCCTCTTC TGTGGCGGAA AACCCTCAAG 
AATTTTGAGG TGGAGAGCTT ATTTCAGAAG TACCACAATC GCCGCAGCTC CCGCATCAAA 
GCTCCCTCAC GCAGCACCAT CCAGAAGTTT GTGTCCCGCA TGTCCAACTC TCACACCTCG 
TGTACCTCCA GTGCCTCACC ATCCAGTAAT GAAAGCAGTA TGAAGGACGA GGAGGAGCAG 
ATGTGTCCCA TCTGTCTGCT GGACATGCTG GATGAAGAAA GTCTGACAGT GTGTGAAGAG 
GGCTGCCGAA ACAAACTCCA CCATCACTGC ATGTCTATAT GGGCCGAGGA GTGCAGAAGA 
AATCACGATC CTTTAATCTG CCCACTGTGT AGAGCCAAAT GGAAGCCTCA TGATTTTTAC 
AGTTATGAAG CGCCATCTAC TGTGGAAAGC ACAACCAGCC GCTCTCAGAG TCAAACAGCT 
GCTTCATCAT CATCATCATC TTCATCTCTT CATGCTGAGA GCTCCAGGAC GGGCCAGGAT 
GGAGACTTCT CTCTTCCTCA ATATGGAGTT CAGCACATCC CTCAGACGTA CACGGAGCTC 
GCCGAACCCT GGATCAAGGT GTTTGGATTG GAGCTGGTCG GCTGCCTCTT CTCTCGTAAC 
TGGAACATCA GAGAAATGGC TCTGCGGCGG CTGTCCCATG ACGTGAGTGG AGCCCTGCTC 
TTGGCCAACG GCGAGCACTC ATTGGCCGGA GCGGGTGCAG GGGCAGGGCT GGCCGAGGTA 
TCTGGAGACG TGGTGGTGGA GTCCTGCTGT AGTGTGCTCT CCATGGTCTG TGCAGACCCT 
GTCTACAAAG TCTACGTAGC AGCGCTGAAA ACCCTGCGAG CGATGCTTGT CTACACCCCA 
TGCCACTCTG CTTCAGAGAG GACTCGATTA CAGCAGCTTC TGCGGCCCGT GGTGGAAACC 
ATACTGGTCA AGTGTGCAGA TGCTAACAGT CGCACCAGTC AGCTGTCCGT GTCCACTCTG 
CTGGAGCTGT GCAAAGGTCA AATGGGAGAA CTCGCTGTGG GCAGAGAAAT TCTCAAAGCA 
GGCTCTATTG GTATTGGTGG AGTTGACTAT GTCCTGAACT GCATCTTGGG CTCTAAAACG 
GAGGCCAATA ACTGGCAAGC ACTGCTGGGT CGGCTGTGTC TGATTGACAG GCTCTTACTG 
GAGTTTCCTG CTGAATTCTA CCCTCACATC GTGTCAGGTG GAGACTGCAA CCAGGCGCAA 
AACGTTGTGG AGAGGTACCA GAAGCTGCTG CCTCTTCTAA GTTTTGCTCT GAAGTCCATT 
GATAACTCTC ACTCCATGGT GGGAAAGCTT TCTCGTCGTG TCTTCCTGAG CGCTGCCCGT 
ATGGTAGCTC GTGTGCCTCA TGTCTTCGTT AAGCTACTGG ACATGCTCAA CGTCACCAGC 
TCCACCCACT ATGCACGCAT GCGCCGGCGT CTTCTGGCCA TTGCGGAGGA GATGGACATC 
CTGGATGCGA TTCATCTGGG ACTAGATGAC CTACAGAAAG AGCACACCTC ACCCTCTTGT 
GCTTTCCTTG AACCCTCAGC CCCTCATAAC TCCCCATGTA CGACTGAAAG AAGCTCCCCC 
ACCCATGGTC TACAGCAGCC CGCCGCTAAG GTGGCACTTT CAGGTCAAGC CAGTTCTACT 
CCTGTAGAGG ACATAACCGA GAGACTCTCA AACATAGTCG CTAGCCCTGC TGAATTTCCA 
AAGCCCCCCA TTCAGGCTCG AAACAGACCC GTTAGTCAGT GTCTGAACTC CCCCTCATCT 
GGTCAACCCC CAAGCCTTCC TTCTCTGTCC ATGGCCAATG CCTCAGCCTT CCCTCCAGAA 
AACCCCAAAG CCCTGCCACA GAGTTTTATT CCCAGCAAGT TTGCCCAGTC CTCACTGGGT 
TCCCAACGCA AGTTGCCTCT TCAGAAAGGT GCCTGTAGTA GTAAAGAACC AGAGAAGCTT 
CCATCCCCTG TGTTTACTCA AGCTCGGCCA CTGCCCCAGA GTCAGATCCA CCGGCCCAGA 
CCTTCTCGAC CCACTCCTGC TGCCTCATCT GATGGAGCTA AAGCTTTAAG CCCTTTGACA 
AAGGGCAACA TGAAACTGGA CCTTCAGGAG GCCCGGTCCA GAGATGATGT GGCCTGCAGT 
GGGGGATCTG CTCTCATCCC CAGTGAGGAC ACTGCCTTCA CGCCAGTGGA GGAGAAGTTC 
CACGGTCTGG ATGCTTCAGC CGATCTCAGC TCCAGCATGG AGGACCTGCT GGAAGCCTCT 
ATCCCTGCGG CAGACAGCAC GGTCACCTTC CAGTCTGAAG TGGCAGTGCT GTCCCCCGAG 
CAGGCAAATG CAAACGATGA CACGTACAGT GAAGACGTGA CCCAGAACCA GAAGTGCAAG 
GAGAAGATGG AAGCTGAAGA AGAGGAGGCA TTGGCTGTAG TCATGGCCAT GTCTGAGTCA 
CAAGACGCCC TACCTGTAAT CCCACAGTTA CAGGTGGACA AAGGGGAGGA CGTGATCATT 
ATTCAGGTGG ATACACCAGA GACTCTGCCT GGTCATACCA AAGCAAAGCA GCCATACAGG 
GAGGGAAACG AATGGCTAAA AGGTCAACAG ATTGGCCTGG GTGCCTTTTC GTCATGTTAC 
CAGGCTCAGG ATGTGGGCAC TGGGACTCTG ATGGCTGTCA AACAGGTGAC GTACGTGAGG 
AACACTTCAT CAGAGCAGGA GGAGGTGGTT GAGGCGCTAC GAGAGGAGAT TCGCATGATG 
GGTCTACTAA ACCACCCACA CATCATCCGC ATGCTGGGGG CAACCTGTGA GAAGAACAAC 
TACAACCTTT TCGTAGAGTG GATGGCAGGT GGCTCTGTAT CTCATCTCTT GAATAAGTAT 
GGCGCATTTA AAGAAGGTGT GATCATCAAC TACACGGAGC AGCTTCTGCG GGGTCTGGCC 
TACCTTCATG AGAACCAGAT CATCCACCGC GACATCAAAG GTGCCAATCT ACTGATTGAC 
AGCACAGGCC AGAGGCTGAG GATAGCTGAT TTTGGGGCTG CAGCACGTTT GGCTTCGAAA 
GGCACTGGAG CAGGGGAGTT CCAGGGTCAA CTTCTGGGAA CCATTGCCTT CATGGCTCCG 
GAGGTGCTGA GAGGACAGCA GTACGGCCGG AGCTGTGATG TCTGGAGTGT TGGCTGTGCC 
ATCATTGAGA TGTCCTGTGC TAAACCTCCC TGGAACGCTG AAAAACACTC CAACCACCTG 
GCGCTCATTT TCAAGATCGC CAGCGCCACC ACAGCGCCCT CCATCCCACC ACACTTGTCT 
CCAGGCCTGC GGGACGTGAC CCTGCGCTGT CTGGAGCTCC AGCCCACCGA CCGGCCGCCC 
TCCAGAGAGC TCCTGAAACA CCCCGTCTTC AGACACAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MAP3K1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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