ascc3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ascc3
Entrez Gene ID	100332599
Full Name	activating signal cointegrator 1 complex subunit 3
Synonyms	si:ch73-374j3.1
General protein information	Preferred Names activating signal cointegrator 1 complex subunit 3 Names activating signal cointegrator 1 complex subunit 3 zmp:0000000854
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017351441.1	XP_017206930.1	activating signal cointegrator 1 complex subunit 3
XM_021466796.1	XP_021322471.1	activating signal cointegrator 1 complex subunit 3

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06124	XP_003711991.1
Danio rerio (zebrafish)	ascc3	XP_002664072.3
Oryza sativa (rice)	Os03g0213400	NP_001049362.1
Drosophila melanogaster (fruit fly)	CG5205	NP_650472.2
Saccharomyces cerevisiae (baker's yeast)	SLH1	NP_011787.4
Neurospora crassa	NCU08267	XP_961895.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001234	XP_321922.5
Bos taurus (cattle)	ASCC3	NP_001193047.1
Mus musculus (house mouse)	Ascc3	NP_932124.2
Arabidopsis thaliana (thale cress)	AT5G61140	NP_001190584.1
Kluyveromyces lactis	KLLA0A09889g	XP_451432.1
Eremothecium gossypii	AGOS_ABR142W	NP_983089.2
Canis lupus familiaris (dog)	ASCC3	XP_854167.1
Gallus gallus (chicken)	ASCC3	XP_419816.1
Schizosaccharomyces pombe (fission yeast)	mug81	NP_596560.1
Homo sapiens (human)	ASCC3	NP_006819.2
Pan troglodytes (chimpanzee)	ASCC3	XP_518652.3
Macaca mulatta (Rhesus monkey)	ASCC3	XP_002803889.1
Rattus norvegicus (Norway rat)	Ascc3	NP_001263986.1
Caenorhabditis elegans (roundworm)	CELE_Y54E2A.4	NP_497060.2

Gene Ontology

The following ascc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ascc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa55442	XM_017351441.1 Latest version!	Danio rerio activating signal cointegrator 1 complex subunit 3 (ascc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa55442	XM_021466796.1 Latest version!	Danio rerio activating signal cointegrator 1 complex subunit 3 (ascc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ascc3 ( XM_017351441.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206930.1 Danio rerio activating signal cointegrator 1 complex subunit 3 (ascc3), mRNA.

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CloneID	ODa55442
Clone ID Related Accession (Same CDS sequence)	XM_017351441.1 , XM_021466796.1
Accession Version	XM_017351441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	activating signal cointegrator 1 complex subunit 3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) and transcript sequence (GDQH01003594.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1330 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTCCTC CTCGGCTCAC TGGAGCCCTG CGCTCCTTCT CTAACGTCAG CAAACAGGAT 
GATTTAACGC AGGACATTTA TGATCTGAAG ACCAAACGCT TAAAACGACA GGAAATATTT 
GCCCGTGATG GTCTGACATG GCAGAAGATA GTTCAGTTCT TCACCCAACA TCTACAGAAG 
AAAGATCAGC CGGTGGCCAC TCAGGAGCTC AAGAACATCC TCCAAGCAGC CAAACAGATC 
GTCGGTACAG ATTTCGGACA AGACACCATT GAAAGTGCCG GGGTCTTCTT GTTTAAAACA 
TTCCACAACA AAGACCAAAT CGGTCATGAG GAAACCCGGG CCATAAAACA CATGTTTGGA 
CCGTTCCCGG CGTCTGCTGC TGAATTGTCC TGTGCAGCGG TGGGCCGTTT AGTGGCTCAG 
ATCGGAGAAT CTCAGGTGGA GGCTTTCATA CAAACGCAGA GTTCCCTCAA GACGCCGGAT 
CGTGGTTCTG TCTTTGGCCG AAACATTGCT TTCTCATTTG ACACCTATGT GCTGGATGCT 
CAAGATGATT TTCCCTGGTC AGATGGGACT GATATGGAAC AGACGGTGGA CTTTCACAGC 
TTTCTCAATA ATAATGTTGG TGAGAGAAGA GTTGGAGGTG TTTACTCTGC AGAAGAACTG 
GAGCAGAGGA ACACAGACGG TTCCATTCTG AGACAGGAGG TGGAGAAATA CCTGAATGAG 
GGAAATATGG TGTCTTCCAG TGCCGAGGAG CTCTCCACCT CTCTTTTCGA AATGCTGGCC 
TCCACAAGAA GCGATGACGA ACTACAAAAC GAGCTCTTTG AGTTTCTGGG TCTTGAGGGG 
TTTGAGATGA TCTCCAGGCT CCTTCAGAGA AGGTCGGACA TTGTTGATGC TTTGGTGTCC 
ATCCCAGCAG ACAGCAGGCA TCACTTCACA TCAGGAAAGC CTAAAGGTCT GCGAGATGAA 
TCCAACATGC CCACATACGG CTGTCAGGTG ACCATCCAGT CTGAGCAGGA GAAACAGATG 
ATGAAGATCT ACAGAAAAGA GGAGAAGAAG GAGAGAAAGC GCGAGAGACG AGGAGATGAT 
GGGGAATCCT CTGAAAGTGC GTTTCATTTT GACCCCATGG AGCTGAGACT GCAGAGGGAG 
CAGGCTTTGC TGGCAGCACG CTCGTCTCCA GTTTTGGGCC GGCACAGAGA TTATGAGCGC 
ATTCGCTACC CTCACGTATA CGATTCTTAC GGCGAAGCCA CCAAAACATC TGCGTTCATC 
GGAGGATCCA AGATGCTGCT GCCCGAGGGG ATCAGAAGAG ACAATAATAA GATGTACGAG 
GAGGTGGAGA TCCCCCCCAA CGAGCCCATG CCCATCGGTT TTGAGGAAAA AGCAGTGTTC 
GTCTCGGAGC TGGACGAGAT CGGCCAGTTG GTGTTCAAGG GCATGAAGAG GCTGAACAGA 
ATCCAGTCTA TAGTGTTCGA GACGGCTTAC AACACCAATG AAAACCTGCT CATATGCGCC 
CCCACCGGTG CCGGAAAAAC CAACATCGCC ATGCTCACAA TACTACACGA GATCCGCCAG 
CACCTACAGC CTGGAGGAGT CATCCGCAAG GACCAGTTTA AGATTGTGTA CGTGGCTCCG 
ATGAAGGCCC TGGCTGCTGA GATGACTAAT TACTTCAGTA AGCGATTGGA GCCTTTGGGC 
ATCGCCGTCA AGGAGCTGAC GGGAGACATG CAGCTGACCA AAGGAGAAAT CCTGCGCACT 
CAGATGTTGG TGACGACTCC AGAGAAGTGG GATGTGGTGA CCAGGAAGAG TGTGGGCGAT 
GTGGCTTTAT CTCAGGTGGT CCGGCTGCTG ATCCTGGATG AAGTTCATCT CCTGCATGAG 
GATAGAGGGC CTGTTCTGGA GAGCCTGGTG GCCCGGACAC TGCGCCAGGT GGAGTCGACC 
CAGAGTATGA TCCGAATACT CGGTCTCTCT GCAACGTTGC CAAATTACCT GGACGTGGCG 
ACTTTCCTCC ATGTGAACCC TTTCATTGGA TTGTTCTACT TCGACAGCCG TTTCCGTCCT 
GTTCCACTTG GCCAGAGCTT CGTTGGCATC AAGACTACAA ATAAGGTCCA ACAGCTGCAC 
GACATGGAGG AGGTTTGTTA TGAAAAAGTC CTGAAGCAGA TTAAAGCGGG TCACCAGGTG 
ATGGTGTTTG TGCATGCCCG AAACTCCACT GTCCGCACCG CCATGAGTCT CATCGAGATG 
GCCAAAAACC GCGGAGAACT GTCCTTCTTT CAGGTCGACC AGGGTGCAGA CTACGGCCAG 
TGTGAGAAAC AGATACAGCG CTCCAGAAAT AAGCAGATGC GAGAGATGTT TCCTGATGGA 
TTCGGGATTC ATCATGCTGG AATGCTGCGT CAGGACCGCT CCCTGATGGA GAGCATGTTT 
TCCAGAGGAT ACCTGAAGGT TCTGGTGTGC ACGGCCACAC TAGCCTGGGG AGTCAACTTA 
CCTGCACATG CCGTCATCAT CAAGGGCACT AATATCTATG ATGCGAAGCG TGGAACTCTG 
GTGGATCTGG GCATCCTGGA TGTGATGCAG ATCTTCGGTC GTGCCGGACG GCCACAGTTT 
GATAAATATG GAGAGGGAAC CATCATCACA ACACACGACA AACTCAGCCA TTACCTGACA 
CTGCTGACCC AGCAGAACCC CATCGAGAGC CAGTTCCAGC AGAGCCTCGC TGACAACCTC 
AACGCTGAGA TCGCTCTCGG GACGGTCACC AATGTCGACG AAGCCGTCCG ATGGTTGAGC 
TACACATACC TGTATGTGCG AATGAGAGCC AATCCACTTG CCTATGGGAT CAACCACAAG 
GCCTATCAGA TGGACCCGCA GCTGGAGCTC TACAGGAAGG AGTTAGTGGT GGAGTCCGGC 
AGGAAGCTGG ATAAAGCACG GATGATCCGA TTCGACGAGA GGACCGGTTA CTTTGCATCT 
ACAGACCTCG GCAGGACAGC CAGTCACTTC TACATTAAAT ACAACACTAT TGAGTCGTTC 
AATGAGCTGT TCAATGCGCA GAACACTGAA GCAGACGTGC TCAGCATCGT CTCCAAAGCT 
GAAGAGTTTG AGCAGATCAA GGTGCGGGAT GAGGAGCTGG AGGAGCTGGA GAAGATGTTG 
TGTAATTTCT GTGAGCTGCC GGCGTCTGGA GGAGTGGAGA ACGGCTACGG CAAGATCAAC 
ATCCTGCTGC AGACCTACAT CAGCCGTGGA GAAGTGGACA GCTTCTCACT CATCTCTGAC 
CTGTCATATG TAGCGCAGAA CGCAGCTCGT ATCATGCGGG CTCTGTTTGA GATGGCTCTG 
AGGAAGCGCT GGCCGGCCAT GACCTACCGC CTGCTGAATC TGTGTAAGGT GATGGACAAG 
CGTCTGTGGG GCTGGGCTCA CCCGCTCAGA CAGTTCAACA CACTTCCGGC TTCAGCGCTG 
GCCCGGATGG AGGACAAGAA CCTCACCATC GACAAACTCA GAGACATGGG CAAAGACGAG 
ATCGGTCACA TGCTGCATCA CGTGAACATC GGTCTGAAGG TGAAGCAGTG TGTTCATCAG 
ATTCCCGCCA TCCTGCTGGA GTCCAGCATT CAGCCAATCA CCCGCACCGT TTTGCGCGTC 
CGCCTCAGCA TCACCCCAGA CTTCAGGTGG AACGACCAGG TCCACGGTTC AGTTGGAGAG 
CCCTGGTGGT TATGGGTAGA GGATCCCATC AATGACCACA TCTACCACTC TGAGTACTTC 
CTGCTGCAGA AGAAACAGGT TGTGAGTGGA GAGCCGCAGC AGGTGGTCTT CACCATCCCC 
ATCTTTGAGC CCATGCCTTC TCAGTATTAC ATCCGCGCCG TGTCTGACCG CTGGCTGGGC 
TCTGAAGCCG TGTGTATCAT CAACTTCCAG CACCTCATTC TGCCAGAAAG ACACCCGCCA 
CATACAGAGC TCTTGGATCT TCAGCCGTTG CCGATTACTG CTTTGGGAAA CAGGGAGTAT 
GAGAGCCTGT ACAAGTTCAC ACACTACAAT CCCATTCAGA CGCAGATCTT CCACACGCTC 
TACCACACAG ACACCAATGT CCTGCTGGGG GCTCCAACCG GCTCCGGAAA AACCATCGCT 
GCAGAGATGG CCATATTCAG GGTCTTCAAC ATGTACCCCA CCTCAAAGGT GGTCTACATT 
GCTCCTCTCA AAGCTCTGGT TCGAGAGAGA ATCGAGGACT GGAAGATCAG AATAGAAGAG 
AAACTTGGGA GAAAGGTGGT TGAGTTAACT GGTGACAATA CTCCGGACAT GCGAGCCATA 
GCACAGGCTG ACCTGATCGT CACGACACCA GAGAAGTGGG ATGGAGTGAG CCGCAGCTGG 
CAGAACCGCA GCTATGTGCA GAAAGTGGCC ATTCTCATCA TTGACGAGAT CCATCTTCTG 
GGTGAAGACC GAGGGCCGGT TCTTGAAGTC ATTGTGTCCA GAACCAACTT TATTTCATCG 
CACACCTCAA AAACGGTCAG AGTAGTAGGA CTGTCCACCG CGCTGGCTAA TGCACGAGAT 
CTAGCCGACT GGCTGGGCAT CGGACAGGTG GGACTGTTTA ATTTCCGGCC CTCTGTTCGC 
CCGGTGCCAT TGGAGGTTCA TATTCAGGGC TTTCCGGGGC AGCATTATTG CCCTAGAATG 
GCCACCATGA ACAAACCAGT ATTCCAAGCA ATCCGTACCC ATTCTCCTGC CAAACCCGTC 
CTGATATTTG TGTCGTCCCG TCGGCAGACG CGGCTCACAG CTCTCGACCT CATTGCCTTC 
CTGGCTACTG AAGATGACCC CAAACAGTGG CTCCACCAGG ACGAGAGAGA GATGACTGAC 
ATCATCGCCA CCATCAGAGA GTCGAACCTG AAGCTGACCC TGGCGTTCGG GATCGGGATG 
CATCATGCAG GCCTTCACGA GCGGGACAGG AAGACGGTGG AAGAACTCTT CGTCAACTGT 
AAGATTCAGG TGTTAATTGC GACGAGCACG CTGGCGTGGG GCGTCAACTT TCCTGCCCAC 
CTGGTGATCG TAAAGGGCAC AGAATACTAC GACGGAAAGA CCAGGCGCTA TGTGGATTAC 
CCCATAACAG ATGTTCTCCA GATGATGGGG CGGGCAGGGC GGCCGCAGTT CGATGACCAG 
GGCAAGGCTG TTATCCTTGT GCACGACATC AAGAAGGACT TTTATAAGAA GTTCCTGTAC 
GAGCCGTTCC CTGTGGAGTC TAGTCTTCTT AGTGTGCTCT CCGACCACTT GAATGCAGAA 
ATCGCAGCAG GCACGGTCAC CTCAAAGCAG GACGCCATGG ATTACATCAC CTGGACGTAT 
TTCTTCCGCA GACTGGTCAT GAACCCTAGC TACTATAATT TGGACGACAT CAGCCATGAA 
ACCATTAATA AATACCTGTC AAACCTGGTG GAGAGGAGTC TGAGGGATCT GGAATGTTCC 
TACTGCATGG AGATACAACA GGACGAGCAA ACCATCGAGC CCCTGACATA CGGCCGCATT 
TCATCCTATT ATTACCTGAA ACACCAGACC ATCCGCATGT TCAAAGAGCG CCTGAAGCCC 
GAGCTGCCGG TCCACGAGTT GCTGGCCATC CTGTCGGAAG CAGAGGAGTA CGCCGAGCTC 
CCAGTACGCC ACAACGAGGA CCAGTTAAAC AGCGAGCTGG CCCAGCGGCT CCCCCTACAG 
GTCAACCCGC ACTCCTATGA CAGCGCACAC ACCAAAACAC ACCTGCTCCT ACAGGCCCAC 
TTCAGCCGCG CACAGCTGCC CTGCTCCGAC TACGGGACCG ACACCAAAAC TGTGCTGGAC 
AACGCCATAC GCATCTGCCA GGCTATGCTG GATGTGGTGG CGAATGAAGG CTGGCTTGTG 
TCCGCGCTGA GTCTCTGTAA TCTGGTCCAG ATGATCATCC AGGCCCGATG GCTGCACGAT 
TCCTCCCTGC TCACACTCCC TCACATCCAG AAGCAGGAGC TCTATGTCTT CAGGAGGTGG 
AGCTCCAGAG GTGTGAGGGC TGGATGTGGC CACCAAGGCC CAATCGAAGG TCTTCCTGAG 
CTGATTGCTG CCTGTGATGG AAAAGAGGAC ATCTTTACGT CTATGGTGAA GGAAGTGCTG 
CAACCCAATC AGATCTCTCA GGCCTGGCAG TATGTGAGTC AGCTGCCGGT GCTGGAGATC 
AGTCTGAGTG TGAAGGGATT CTGGGAAAAC TCTGTGGAGC AGACAGAGCG GGCGGTTCCG 
GCTCAGGTGT CCAGCATTAG GGATGAGTCC AGCTGGCTGC CGCTGCATGC GGATCAGGAG 
TACGTCCTGC AGGTGTCCAT GCGCCGGCTC AATGCGGGAC AGCACAGGAG GAAGCAGGAC 
AGCAGAGCTC AGGCGCCGCG TTTCCCCAAA CCCAAAGATG AAGGCTGGTT TCTGGTCCTC 
GGGGAGGTGG AGAAGAAAGA GCTGCTGGCC GTCAAGCGCG TGGGCTTTGT TCGCAACCAC 
AGCAGCGTCT CTGTGGCCTT CTACACTCCC GAGAAGACTG GAAAGTGTAT CTACACGCTG 
TACCTGATGA GCGACAGTTA CCTGGGCCTG GATCAGCAGT ACGACATGCA TCTCAATGTC 
ATCCCGGCCA GCATCGCTGC ACAGGTCAAC AGTGAAGTAG CTGATAGTGT AGGTAAACTG 
GCCCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206930.1
CDS	128..6739
Translation	MSPPRLTGALRSFSNVSKQDDLTQDIYDLKTKRLKRQEIFARDGLTWQKIVQFFTQHLQKKDQPVATQELKNILQAAKQIVGTDFGQDTIESAGVFLFKTFHNKDQIGHEETRAIKHMFGPFPASAAELSCAAVGRLVAQIGESQVEAFIQTQSSLKTPDRGSVFGRNIAFSFDTYVLDAQDDFPWSDGTDMEQTVDFHSFLNNNVGERRVGGVYSAEELEQRNTDGSILRQEVEKYLNEGNMVSSSAEELSTSLFEMLASTRSDDELQNELFEFLGLEGFEMISRLLQRRSDIVDALVSIPADSRHHFTSGKPKGLRDESNMPTYGCQVTIQSEQEKQMMKIYRKEEKKERKRERRGDDGESSESAFHFDPMELRLQREQALLAARSSPVLGRHRDYERIRYPHVYDSYGEATKTSAFIGGSKMLLPEGIRRDNNKMYEEVEIPPNEPMPIGFEEKAVFVSELDEIGQLVFKGMKRLNRIQSIVFETAYNTNENLLICAPTGAGKTNIAMLTILHEIRQHLQPGGVIRKDQFKIVYVAPMKALAAEMTNYFSKRLEPLGIAVKELTGDMQLTKGEILRTQMLVTTPEKWDVVTRKSVGDVALSQVVRLLILDEVHLLHEDRGPVLESLVARTLRQVESTQSMIRILGLSATLPNYLDVATFLHVNPFIGLFYFDSRFRPVPLGQSFVGIKTTNKVQQLHDMEEVCYEKVLKQIKAGHQVMVFVHARNSTVRTAMSLIEMAKNRGELSFFQVDQGADYGQCEKQIQRSRNKQMREMFPDGFGIHHAGMLRQDRSLMESMFSRGYLKVLVCTATLAWGVNLPAHAVIIKGTNIYDAKRGTLVDLGILDVMQIFGRAGRPQFDKYGEGTIITTHDKLSHYLTLLTQQNPIESQFQQSLADNLNAEIALGTVTNVDEAVRWLSYTYLYVRMRANPLAYGINHKAYQMDPQLELYRKELVVESGRKLDKARMIRFDERTGYFASTDLGRTASHFYIKYNTIESFNELFNAQNTEADVLSIVSKAEEFEQIKVRDEELEELEKMLCNFCELPASGGVENGYGKINILLQTYISRGEVDSFSLISDLSYVAQNAARIMRALFEMALRKRWPAMTYRLLNLCKVMDKRLWGWAHPLRQFNTLPASALARMEDKNLTIDKLRDMGKDEIGHMLHHVNIGLKVKQCVHQIPAILLESSIQPITRTVLRVRLSITPDFRWNDQVHGSVGEPWWLWVEDPINDHIYHSEYFLLQKKQVVSGEPQQVVFTIPIFEPMPSQYYIRAVSDRWLGSEAVCIINFQHLILPERHPPHTELLDLQPLPITALGNREYESLYKFTHYNPIQTQIFHTLYHTDTNVLLGAPTGSGKTIAAEMAIFRVFNMYPTSKVVYIAPLKALVRERIEDWKIRIEEKLGRKVVELTGDNTPDMRAIAQADLIVTTPEKWDGVSRSWQNRSYVQKVAILIIDEIHLLGEDRGPVLEVIVSRTNFISSHTSKTVRVVGLSTALANARDLADWLGIGQVGLFNFRPSVRPVPLEVHIQGFPGQHYCPRMATMNKPVFQAIRTHSPAKPVLIFVSSRRQTRLTALDLIAFLATEDDPKQWLHQDEREMTDIIATIRESNLKLTLAFGIGMHHAGLHERDRKTVEELFVNCKIQVLIATSTLAWGVNFPAHLVIVKGTEYYDGKTRRYVDYPITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKFLYEPFPVESSLLSVLSDHLNAEIAAGTVTSKQDAMDYITWTYFFRRLVMNPSYYNLDDISHETINKYLSNLVERSLRDLECSYCMEIQQDEQTIEPLTYGRISSYYYLKHQTIRMFKERLKPELPVHELLAILSEAEEYAELPVRHNEDQLNSELAQRLPLQVNPHSYDSAHTKTHLLLQAHFSRAQLPCSDYGTDTKTVLDNAIRICQAMLDVVANEGWLVSALSLCNLVQMIIQARWLHDSSLLTLPHIQKQELYVFRRWSSRGVRAGCGHQGPIEGLPELIAACDGKEDIFTSMVKEVLQPNQISQAWQYVSQLPVLEISLSVKGFWENSVEQTERAVPAQVSSIRDESSWLPLHADQEYVLQVSMRRLNAGQHRRKQDSRAQAPRFPKPKDEGWFLVLGEVEKKELLAVKRVGFVRNHSSVSVAFYTPEKTGKCIYTLYLMSDSYLGLDQQYDMHLNVIPASIAAQVNSEVADSVGKLALR

Target ORF information:

RefSeq Version	XM_017351441.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio activating signal cointegrator 1 complex subunit 3 (ascc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351441.1

ORF Insert Sequence:

ATGTCTCCTC CTCGGCTCAC TGGAGCCCTG CGCTCCTTCT CTAACGTCAG CAAACAGGAT 
GATTTAACGC AGGACATTTA TGATCTGAAG ACCAAACGCT TAAAACGACA GGAAATATTT 
GCCCGTGATG GTCTGACATG GCAGAAGATA GTTCAGTTCT TCACCCAACA TCTACAGAAG 
AAAGATCAGC CGGTGGCCAC TCAGGAGCTC AAGAACATCC TCCAAGCAGC CAAACAGATC 
GTCGGTACAG ATTTCGGACA AGACACCATT GAAAGTGCCG GGGTCTTCTT GTTTAAAACA 
TTCCACAACA AAGACCAAAT CGGTCATGAG GAAACCCGGG CCATAAAACA CATGTTTGGA 
CCGTTCCCGG CGTCTGCTGC TGAATTGTCC TGTGCAGCGG TGGGCCGTTT AGTGGCTCAG 
ATCGGAGAAT CTCAGGTGGA GGCTTTCATA CAAACGCAGA GTTCCCTCAA GACGCCGGAT 
CGTGGTTCTG TCTTTGGCCG AAACATTGCT TTCTCATTTG ACACCTATGT GCTGGATGCT 
CAAGATGATT TTCCCTGGTC AGATGGGACT GATATGGAAC AGACGGTGGA CTTTCACAGC 
TTTCTCAATA ATAATGTTGG TGAGAGAAGA GTTGGAGGTG TTTACTCTGC AGAAGAACTG 
GAGCAGAGGA ACACAGACGG TTCCATTCTG AGACAGGAGG TGGAGAAATA CCTGAATGAG 
GGAAATATGG TGTCTTCCAG TGCCGAGGAG CTCTCCACCT CTCTTTTCGA AATGCTGGCC 
TCCACAAGAA GCGATGACGA ACTACAAAAC GAGCTCTTTG AGTTTCTGGG TCTTGAGGGG 
TTTGAGATGA TCTCCAGGCT CCTTCAGAGA AGGTCGGACA TTGTTGATGC TTTGGTGTCC 
ATCCCAGCAG ACAGCAGGCA TCACTTCACA TCAGGAAAGC CTAAAGGTCT GCGAGATGAA 
TCCAACATGC CCACATACGG CTGTCAGGTG ACCATCCAGT CTGAGCAGGA GAAACAGATG 
ATGAAGATCT ACAGAAAAGA GGAGAAGAAG GAGAGAAAGC GCGAGAGACG AGGAGATGAT 
GGGGAATCCT CTGAAAGTGC GTTTCATTTT GACCCCATGG AGCTGAGACT GCAGAGGGAG 
CAGGCTTTGC TGGCAGCACG CTCGTCTCCA GTTTTGGGCC GGCACAGAGA TTATGAGCGC 
ATTCGCTACC CTCACGTATA CGATTCTTAC GGCGAAGCCA CCAAAACATC TGCGTTCATC 
GGAGGATCCA AGATGCTGCT GCCCGAGGGG ATCAGAAGAG ACAATAATAA GATGTACGAG 
GAGGTGGAGA TCCCCCCCAA CGAGCCCATG CCCATCGGTT TTGAGGAAAA AGCAGTGTTC 
GTCTCGGAGC TGGACGAGAT CGGCCAGTTG GTGTTCAAGG GCATGAAGAG GCTGAACAGA 
ATCCAGTCTA TAGTGTTCGA GACGGCTTAC AACACCAATG AAAACCTGCT CATATGCGCC 
CCCACCGGTG CCGGAAAAAC CAACATCGCC ATGCTCACAA TACTACACGA GATCCGCCAG 
CACCTACAGC CTGGAGGAGT CATCCGCAAG GACCAGTTTA AGATTGTGTA CGTGGCTCCG 
ATGAAGGCCC TGGCTGCTGA GATGACTAAT TACTTCAGTA AGCGATTGGA GCCTTTGGGC 
ATCGCCGTCA AGGAGCTGAC GGGAGACATG CAGCTGACCA AAGGAGAAAT CCTGCGCACT 
CAGATGTTGG TGACGACTCC AGAGAAGTGG GATGTGGTGA CCAGGAAGAG TGTGGGCGAT 
GTGGCTTTAT CTCAGGTGGT CCGGCTGCTG ATCCTGGATG AAGTTCATCT CCTGCATGAG 
GATAGAGGGC CTGTTCTGGA GAGCCTGGTG GCCCGGACAC TGCGCCAGGT GGAGTCGACC 
CAGAGTATGA TCCGAATACT CGGTCTCTCT GCAACGTTGC CAAATTACCT GGACGTGGCG 
ACTTTCCTCC ATGTGAACCC TTTCATTGGA TTGTTCTACT TCGACAGCCG TTTCCGTCCT 
GTTCCACTTG GCCAGAGCTT CGTTGGCATC AAGACTACAA ATAAGGTCCA ACAGCTGCAC 
GACATGGAGG AGGTTTGTTA TGAAAAAGTC CTGAAGCAGA TTAAAGCGGG TCACCAGGTG 
ATGGTGTTTG TGCATGCCCG AAACTCCACT GTCCGCACCG CCATGAGTCT CATCGAGATG 
GCCAAAAACC GCGGAGAACT GTCCTTCTTT CAGGTCGACC AGGGTGCAGA CTACGGCCAG 
TGTGAGAAAC AGATACAGCG CTCCAGAAAT AAGCAGATGC GAGAGATGTT TCCTGATGGA 
TTCGGGATTC ATCATGCTGG AATGCTGCGT CAGGACCGCT CCCTGATGGA GAGCATGTTT 
TCCAGAGGAT ACCTGAAGGT TCTGGTGTGC ACGGCCACAC TAGCCTGGGG AGTCAACTTA 
CCTGCACATG CCGTCATCAT CAAGGGCACT AATATCTATG ATGCGAAGCG TGGAACTCTG 
GTGGATCTGG GCATCCTGGA TGTGATGCAG ATCTTCGGTC GTGCCGGACG GCCACAGTTT 
GATAAATATG GAGAGGGAAC CATCATCACA ACACACGACA AACTCAGCCA TTACCTGACA 
CTGCTGACCC AGCAGAACCC CATCGAGAGC CAGTTCCAGC AGAGCCTCGC TGACAACCTC 
AACGCTGAGA TCGCTCTCGG GACGGTCACC AATGTCGACG AAGCCGTCCG ATGGTTGAGC 
TACACATACC TGTATGTGCG AATGAGAGCC AATCCACTTG CCTATGGGAT CAACCACAAG 
GCCTATCAGA TGGACCCGCA GCTGGAGCTC TACAGGAAGG AGTTAGTGGT GGAGTCCGGC 
AGGAAGCTGG ATAAAGCACG GATGATCCGA TTCGACGAGA GGACCGGTTA CTTTGCATCT 
ACAGACCTCG GCAGGACAGC CAGTCACTTC TACATTAAAT ACAACACTAT TGAGTCGTTC 
AATGAGCTGT TCAATGCGCA GAACACTGAA GCAGACGTGC TCAGCATCGT CTCCAAAGCT 
GAAGAGTTTG AGCAGATCAA GGTGCGGGAT GAGGAGCTGG AGGAGCTGGA GAAGATGTTG 
TGTAATTTCT GTGAGCTGCC GGCGTCTGGA GGAGTGGAGA ACGGCTACGG CAAGATCAAC 
ATCCTGCTGC AGACCTACAT CAGCCGTGGA GAAGTGGACA GCTTCTCACT CATCTCTGAC 
CTGTCATATG TAGCGCAGAA CGCAGCTCGT ATCATGCGGG CTCTGTTTGA GATGGCTCTG 
AGGAAGCGCT GGCCGGCCAT GACCTACCGC CTGCTGAATC TGTGTAAGGT GATGGACAAG 
CGTCTGTGGG GCTGGGCTCA CCCGCTCAGA CAGTTCAACA CACTTCCGGC TTCAGCGCTG 
GCCCGGATGG AGGACAAGAA CCTCACCATC GACAAACTCA GAGACATGGG CAAAGACGAG 
ATCGGTCACA TGCTGCATCA CGTGAACATC GGTCTGAAGG TGAAGCAGTG TGTTCATCAG 
ATTCCCGCCA TCCTGCTGGA GTCCAGCATT CAGCCAATCA CCCGCACCGT TTTGCGCGTC 
CGCCTCAGCA TCACCCCAGA CTTCAGGTGG AACGACCAGG TCCACGGTTC AGTTGGAGAG 
CCCTGGTGGT TATGGGTAGA GGATCCCATC AATGACCACA TCTACCACTC TGAGTACTTC 
CTGCTGCAGA AGAAACAGGT TGTGAGTGGA GAGCCGCAGC AGGTGGTCTT CACCATCCCC 
ATCTTTGAGC CCATGCCTTC TCAGTATTAC ATCCGCGCCG TGTCTGACCG CTGGCTGGGC 
TCTGAAGCCG TGTGTATCAT CAACTTCCAG CACCTCATTC TGCCAGAAAG ACACCCGCCA 
CATACAGAGC TCTTGGATCT TCAGCCGTTG CCGATTACTG CTTTGGGAAA CAGGGAGTAT 
GAGAGCCTGT ACAAGTTCAC ACACTACAAT CCCATTCAGA CGCAGATCTT CCACACGCTC 
TACCACACAG ACACCAATGT CCTGCTGGGG GCTCCAACCG GCTCCGGAAA AACCATCGCT 
GCAGAGATGG CCATATTCAG GGTCTTCAAC ATGTACCCCA CCTCAAAGGT GGTCTACATT 
GCTCCTCTCA AAGCTCTGGT TCGAGAGAGA ATCGAGGACT GGAAGATCAG AATAGAAGAG 
AAACTTGGGA GAAAGGTGGT TGAGTTAACT GGTGACAATA CTCCGGACAT GCGAGCCATA 
GCACAGGCTG ACCTGATCGT CACGACACCA GAGAAGTGGG ATGGAGTGAG CCGCAGCTGG 
CAGAACCGCA GCTATGTGCA GAAAGTGGCC ATTCTCATCA TTGACGAGAT CCATCTTCTG 
GGTGAAGACC GAGGGCCGGT TCTTGAAGTC ATTGTGTCCA GAACCAACTT TATTTCATCG 
CACACCTCAA AAACGGTCAG AGTAGTAGGA CTGTCCACCG CGCTGGCTAA TGCACGAGAT 
CTAGCCGACT GGCTGGGCAT CGGACAGGTG GGACTGTTTA ATTTCCGGCC CTCTGTTCGC 
CCGGTGCCAT TGGAGGTTCA TATTCAGGGC TTTCCGGGGC AGCATTATTG CCCTAGAATG 
GCCACCATGA ACAAACCAGT ATTCCAAGCA ATCCGTACCC ATTCTCCTGC CAAACCCGTC 
CTGATATTTG TGTCGTCCCG TCGGCAGACG CGGCTCACAG CTCTCGACCT CATTGCCTTC 
CTGGCTACTG AAGATGACCC CAAACAGTGG CTCCACCAGG ACGAGAGAGA GATGACTGAC 
ATCATCGCCA CCATCAGAGA GTCGAACCTG AAGCTGACCC TGGCGTTCGG GATCGGGATG 
CATCATGCAG GCCTTCACGA GCGGGACAGG AAGACGGTGG AAGAACTCTT CGTCAACTGT 
AAGATTCAGG TGTTAATTGC GACGAGCACG CTGGCGTGGG GCGTCAACTT TCCTGCCCAC 
CTGGTGATCG TAAAGGGCAC AGAATACTAC GACGGAAAGA CCAGGCGCTA TGTGGATTAC 
CCCATAACAG ATGTTCTCCA GATGATGGGG CGGGCAGGGC GGCCGCAGTT CGATGACCAG 
GGCAAGGCTG TTATCCTTGT GCACGACATC AAGAAGGACT TTTATAAGAA GTTCCTGTAC 
GAGCCGTTCC CTGTGGAGTC TAGTCTTCTT AGTGTGCTCT CCGACCACTT GAATGCAGAA 
ATCGCAGCAG GCACGGTCAC CTCAAAGCAG GACGCCATGG ATTACATCAC CTGGACGTAT 
TTCTTCCGCA GACTGGTCAT GAACCCTAGC TACTATAATT TGGACGACAT CAGCCATGAA 
ACCATTAATA AATACCTGTC AAACCTGGTG GAGAGGAGTC TGAGGGATCT GGAATGTTCC 
TACTGCATGG AGATACAACA GGACGAGCAA ACCATCGAGC CCCTGACATA CGGCCGCATT 
TCATCCTATT ATTACCTGAA ACACCAGACC ATCCGCATGT TCAAAGAGCG CCTGAAGCCC 
GAGCTGCCGG TCCACGAGTT GCTGGCCATC CTGTCGGAAG CAGAGGAGTA CGCCGAGCTC 
CCAGTACGCC ACAACGAGGA CCAGTTAAAC AGCGAGCTGG CCCAGCGGCT CCCCCTACAG 
GTCAACCCGC ACTCCTATGA CAGCGCACAC ACCAAAACAC ACCTGCTCCT ACAGGCCCAC 
TTCAGCCGCG CACAGCTGCC CTGCTCCGAC TACGGGACCG ACACCAAAAC TGTGCTGGAC 
AACGCCATAC GCATCTGCCA GGCTATGCTG GATGTGGTGG CGAATGAAGG CTGGCTTGTG 
TCCGCGCTGA GTCTCTGTAA TCTGGTCCAG ATGATCATCC AGGCCCGATG GCTGCACGAT 
TCCTCCCTGC TCACACTCCC TCACATCCAG AAGCAGGAGC TCTATGTCTT CAGGAGGTGG 
AGCTCCAGAG GTGTGAGGGC TGGATGTGGC CACCAAGGCC CAATCGAAGG TCTTCCTGAG 
CTGATTGCTG CCTGTGATGG AAAAGAGGAC ATCTTTACGT CTATGGTGAA GGAAGTGCTG 
CAACCCAATC AGATCTCTCA GGCCTGGCAG TATGTGAGTC AGCTGCCGGT GCTGGAGATC 
AGTCTGAGTG TGAAGGGATT CTGGGAAAAC TCTGTGGAGC AGACAGAGCG GGCGGTTCCG 
GCTCAGGTGT CCAGCATTAG GGATGAGTCC AGCTGGCTGC CGCTGCATGC GGATCAGGAG 
TACGTCCTGC AGGTGTCCAT GCGCCGGCTC AATGCGGGAC AGCACAGGAG GAAGCAGGAC 
AGCAGAGCTC AGGCGCCGCG TTTCCCCAAA CCCAAAGATG AAGGCTGGTT TCTGGTCCTC 
GGGGAGGTGG AGAAGAAAGA GCTGCTGGCC GTCAAGCGCG TGGGCTTTGT TCGCAACCAC 
AGCAGCGTCT CTGTGGCCTT CTACACTCCC GAGAAGACTG GAAAGTGTAT CTACACGCTG 
TACCTGATGA GCGACAGTTA CCTGGGCCTG GATCAGCAGT ACGACATGCA TCTCAATGTC 
ATCCCGGCCA GCATCGCTGC ACAGGTCAAC AGTGAAGTAG CTGATAGTGT AGGTAAACTG 
GCCCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ascc3 ( XM_021466796.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322471.1 Danio rerio activating signal cointegrator 1 complex subunit 3 (ascc3), mRNA.

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CloneID	ODa55442
Clone ID Related Accession (Same CDS sequence)	XM_017351441.1 , XM_021466796.1
Accession Version	XM_021466796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	activating signal cointegrator 1 complex subunit 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) and transcript sequences (GDQH01003594.1 and GDQQ01001343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 933 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTCCTC CTCGGCTCAC TGGAGCCCTG CGCTCCTTCT CTAACGTCAG CAAACAGGAT 
GATTTAACGC AGGACATTTA TGATCTGAAG ACCAAACGCT TAAAACGACA GGAAATATTT 
GCCCGTGATG GTCTGACATG GCAGAAGATA GTTCAGTTCT TCACCCAACA TCTACAGAAG 
AAAGATCAGC CGGTGGCCAC TCAGGAGCTC AAGAACATCC TCCAAGCAGC CAAACAGATC 
GTCGGTACAG ATTTCGGACA AGACACCATT GAAAGTGCCG GGGTCTTCTT GTTTAAAACA 
TTCCACAACA AAGACCAAAT CGGTCATGAG GAAACCCGGG CCATAAAACA CATGTTTGGA 
CCGTTCCCGG CGTCTGCTGC TGAATTGTCC TGTGCAGCGG TGGGCCGTTT AGTGGCTCAG 
ATCGGAGAAT CTCAGGTGGA GGCTTTCATA CAAACGCAGA GTTCCCTCAA GACGCCGGAT 
CGTGGTTCTG TCTTTGGCCG AAACATTGCT TTCTCATTTG ACACCTATGT GCTGGATGCT 
CAAGATGATT TTCCCTGGTC AGATGGGACT GATATGGAAC AGACGGTGGA CTTTCACAGC 
TTTCTCAATA ATAATGTTGG TGAGAGAAGA GTTGGAGGTG TTTACTCTGC AGAAGAACTG 
GAGCAGAGGA ACACAGACGG TTCCATTCTG AGACAGGAGG TGGAGAAATA CCTGAATGAG 
GGAAATATGG TGTCTTCCAG TGCCGAGGAG CTCTCCACCT CTCTTTTCGA AATGCTGGCC 
TCCACAAGAA GCGATGACGA ACTACAAAAC GAGCTCTTTG AGTTTCTGGG TCTTGAGGGG 
TTTGAGATGA TCTCCAGGCT CCTTCAGAGA AGGTCGGACA TTGTTGATGC TTTGGTGTCC 
ATCCCAGCAG ACAGCAGGCA TCACTTCACA TCAGGAAAGC CTAAAGGTCT GCGAGATGAA 
TCCAACATGC CCACATACGG CTGTCAGGTG ACCATCCAGT CTGAGCAGGA GAAACAGATG 
ATGAAGATCT ACAGAAAAGA GGAGAAGAAG GAGAGAAAGC GCGAGAGACG AGGAGATGAT 
GGGGAATCCT CTGAAAGTGC GTTTCATTTT GACCCCATGG AGCTGAGACT GCAGAGGGAG 
CAGGCTTTGC TGGCAGCACG CTCGTCTCCA GTTTTGGGCC GGCACAGAGA TTATGAGCGC 
ATTCGCTACC CTCACGTATA CGATTCTTAC GGCGAAGCCA CCAAAACATC TGCGTTCATC 
GGAGGATCCA AGATGCTGCT GCCCGAGGGG ATCAGAAGAG ACAATAATAA GATGTACGAG 
GAGGTGGAGA TCCCCCCCAA CGAGCCCATG CCCATCGGTT TTGAGGAAAA AGCAGTGTTC 
GTCTCGGAGC TGGACGAGAT CGGCCAGTTG GTGTTCAAGG GCATGAAGAG GCTGAACAGA 
ATCCAGTCTA TAGTGTTCGA GACGGCTTAC AACACCAATG AAAACCTGCT CATATGCGCC 
CCCACCGGTG CCGGAAAAAC CAACATCGCC ATGCTCACAA TACTACACGA GATCCGCCAG 
CACCTACAGC CTGGAGGAGT CATCCGCAAG GACCAGTTTA AGATTGTGTA CGTGGCTCCG 
ATGAAGGCCC TGGCTGCTGA GATGACTAAT TACTTCAGTA AGCGATTGGA GCCTTTGGGC 
ATCGCCGTCA AGGAGCTGAC GGGAGACATG CAGCTGACCA AAGGAGAAAT CCTGCGCACT 
CAGATGTTGG TGACGACTCC AGAGAAGTGG GATGTGGTGA CCAGGAAGAG TGTGGGCGAT 
GTGGCTTTAT CTCAGGTGGT CCGGCTGCTG ATCCTGGATG AAGTTCATCT CCTGCATGAG 
GATAGAGGGC CTGTTCTGGA GAGCCTGGTG GCCCGGACAC TGCGCCAGGT GGAGTCGACC 
CAGAGTATGA TCCGAATACT CGGTCTCTCT GCAACGTTGC CAAATTACCT GGACGTGGCG 
ACTTTCCTCC ATGTGAACCC TTTCATTGGA TTGTTCTACT TCGACAGCCG TTTCCGTCCT 
GTTCCACTTG GCCAGAGCTT CGTTGGCATC AAGACTACAA ATAAGGTCCA ACAGCTGCAC 
GACATGGAGG AGGTTTGTTA TGAAAAAGTC CTGAAGCAGA TTAAAGCGGG TCACCAGGTG 
ATGGTGTTTG TGCATGCCCG AAACTCCACT GTCCGCACCG CCATGAGTCT CATCGAGATG 
GCCAAAAACC GCGGAGAACT GTCCTTCTTT CAGGTCGACC AGGGTGCAGA CTACGGCCAG 
TGTGAGAAAC AGATACAGCG CTCCAGAAAT AAGCAGATGC GAGAGATGTT TCCTGATGGA 
TTCGGGATTC ATCATGCTGG AATGCTGCGT CAGGACCGCT CCCTGATGGA GAGCATGTTT 
TCCAGAGGAT ACCTGAAGGT TCTGGTGTGC ACGGCCACAC TAGCCTGGGG AGTCAACTTA 
CCTGCACATG CCGTCATCAT CAAGGGCACT AATATCTATG ATGCGAAGCG TGGAACTCTG 
GTGGATCTGG GCATCCTGGA TGTGATGCAG ATCTTCGGTC GTGCCGGACG GCCACAGTTT 
GATAAATATG GAGAGGGAAC CATCATCACA ACACACGACA AACTCAGCCA TTACCTGACA 
CTGCTGACCC AGCAGAACCC CATCGAGAGC CAGTTCCAGC AGAGCCTCGC TGACAACCTC 
AACGCTGAGA TCGCTCTCGG GACGGTCACC AATGTCGACG AAGCCGTCCG ATGGTTGAGC 
TACACATACC TGTATGTGCG AATGAGAGCC AATCCACTTG CCTATGGGAT CAACCACAAG 
GCCTATCAGA TGGACCCGCA GCTGGAGCTC TACAGGAAGG AGTTAGTGGT GGAGTCCGGC 
AGGAAGCTGG ATAAAGCACG GATGATCCGA TTCGACGAGA GGACCGGTTA CTTTGCATCT 
ACAGACCTCG GCAGGACAGC CAGTCACTTC TACATTAAAT ACAACACTAT TGAGTCGTTC 
AATGAGCTGT TCAATGCGCA GAACACTGAA GCAGACGTGC TCAGCATCGT CTCCAAAGCT 
GAAGAGTTTG AGCAGATCAA GGTGCGGGAT GAGGAGCTGG AGGAGCTGGA GAAGATGTTG 
TGTAATTTCT GTGAGCTGCC GGCGTCTGGA GGAGTGGAGA ACGGCTACGG CAAGATCAAC 
ATCCTGCTGC AGACCTACAT CAGCCGTGGA GAAGTGGACA GCTTCTCACT CATCTCTGAC 
CTGTCATATG TAGCGCAGAA CGCAGCTCGT ATCATGCGGG CTCTGTTTGA GATGGCTCTG 
AGGAAGCGCT GGCCGGCCAT GACCTACCGC CTGCTGAATC TGTGTAAGGT GATGGACAAG 
CGTCTGTGGG GCTGGGCTCA CCCGCTCAGA CAGTTCAACA CACTTCCGGC TTCAGCGCTG 
GCCCGGATGG AGGACAAGAA CCTCACCATC GACAAACTCA GAGACATGGG CAAAGACGAG 
ATCGGTCACA TGCTGCATCA CGTGAACATC GGTCTGAAGG TGAAGCAGTG TGTTCATCAG 
ATTCCCGCCA TCCTGCTGGA GTCCAGCATT CAGCCAATCA CCCGCACCGT TTTGCGCGTC 
CGCCTCAGCA TCACCCCAGA CTTCAGGTGG AACGACCAGG TCCACGGTTC AGTTGGAGAG 
CCCTGGTGGT TATGGGTAGA GGATCCCATC AATGACCACA TCTACCACTC TGAGTACTTC 
CTGCTGCAGA AGAAACAGGT TGTGAGTGGA GAGCCGCAGC AGGTGGTCTT CACCATCCCC 
ATCTTTGAGC CCATGCCTTC TCAGTATTAC ATCCGCGCCG TGTCTGACCG CTGGCTGGGC 
TCTGAAGCCG TGTGTATCAT CAACTTCCAG CACCTCATTC TGCCAGAAAG ACACCCGCCA 
CATACAGAGC TCTTGGATCT TCAGCCGTTG CCGATTACTG CTTTGGGAAA CAGGGAGTAT 
GAGAGCCTGT ACAAGTTCAC ACACTACAAT CCCATTCAGA CGCAGATCTT CCACACGCTC 
TACCACACAG ACACCAATGT CCTGCTGGGG GCTCCAACCG GCTCCGGAAA AACCATCGCT 
GCAGAGATGG CCATATTCAG GGTCTTCAAC ATGTACCCCA CCTCAAAGGT GGTCTACATT 
GCTCCTCTCA AAGCTCTGGT TCGAGAGAGA ATCGAGGACT GGAAGATCAG AATAGAAGAG 
AAACTTGGGA GAAAGGTGGT TGAGTTAACT GGTGACAATA CTCCGGACAT GCGAGCCATA 
GCACAGGCTG ACCTGATCGT CACGACACCA GAGAAGTGGG ATGGAGTGAG CCGCAGCTGG 
CAGAACCGCA GCTATGTGCA GAAAGTGGCC ATTCTCATCA TTGACGAGAT CCATCTTCTG 
GGTGAAGACC GAGGGCCGGT TCTTGAAGTC ATTGTGTCCA GAACCAACTT TATTTCATCG 
CACACCTCAA AAACGGTCAG AGTAGTAGGA CTGTCCACCG CGCTGGCTAA TGCACGAGAT 
CTAGCCGACT GGCTGGGCAT CGGACAGGTG GGACTGTTTA ATTTCCGGCC CTCTGTTCGC 
CCGGTGCCAT TGGAGGTTCA TATTCAGGGC TTTCCGGGGC AGCATTATTG CCCTAGAATG 
GCCACCATGA ACAAACCAGT ATTCCAAGCA ATCCGTACCC ATTCTCCTGC CAAACCCGTC 
CTGATATTTG TGTCGTCCCG TCGGCAGACG CGGCTCACAG CTCTCGACCT CATTGCCTTC 
CTGGCTACTG AAGATGACCC CAAACAGTGG CTCCACCAGG ACGAGAGAGA GATGACTGAC 
ATCATCGCCA CCATCAGAGA GTCGAACCTG AAGCTGACCC TGGCGTTCGG GATCGGGATG 
CATCATGCAG GCCTTCACGA GCGGGACAGG AAGACGGTGG AAGAACTCTT CGTCAACTGT 
AAGATTCAGG TGTTAATTGC GACGAGCACG CTGGCGTGGG GCGTCAACTT TCCTGCCCAC 
CTGGTGATCG TAAAGGGCAC AGAATACTAC GACGGAAAGA CCAGGCGCTA TGTGGATTAC 
CCCATAACAG ATGTTCTCCA GATGATGGGG CGGGCAGGGC GGCCGCAGTT CGATGACCAG 
GGCAAGGCTG TTATCCTTGT GCACGACATC AAGAAGGACT TTTATAAGAA GTTCCTGTAC 
GAGCCGTTCC CTGTGGAGTC TAGTCTTCTT AGTGTGCTCT CCGACCACTT GAATGCAGAA 
ATCGCAGCAG GCACGGTCAC CTCAAAGCAG GACGCCATGG ATTACATCAC CTGGACGTAT 
TTCTTCCGCA GACTGGTCAT GAACCCTAGC TACTATAATT TGGACGACAT CAGCCATGAA 
ACCATTAATA AATACCTGTC AAACCTGGTG GAGAGGAGTC TGAGGGATCT GGAATGTTCC 
TACTGCATGG AGATACAACA GGACGAGCAA ACCATCGAGC CCCTGACATA CGGCCGCATT 
TCATCCTATT ATTACCTGAA ACACCAGACC ATCCGCATGT TCAAAGAGCG CCTGAAGCCC 
GAGCTGCCGG TCCACGAGTT GCTGGCCATC CTGTCGGAAG CAGAGGAGTA CGCCGAGCTC 
CCAGTACGCC ACAACGAGGA CCAGTTAAAC AGCGAGCTGG CCCAGCGGCT CCCCCTACAG 
GTCAACCCGC ACTCCTATGA CAGCGCACAC ACCAAAACAC ACCTGCTCCT ACAGGCCCAC 
TTCAGCCGCG CACAGCTGCC CTGCTCCGAC TACGGGACCG ACACCAAAAC TGTGCTGGAC 
AACGCCATAC GCATCTGCCA GGCTATGCTG GATGTGGTGG CGAATGAAGG CTGGCTTGTG 
TCCGCGCTGA GTCTCTGTAA TCTGGTCCAG ATGATCATCC AGGCCCGATG GCTGCACGAT 
TCCTCCCTGC TCACACTCCC TCACATCCAG AAGCAGGAGC TCTATGTCTT CAGGAGGTGG 
AGCTCCAGAG GTGTGAGGGC TGGATGTGGC CACCAAGGCC CAATCGAAGG TCTTCCTGAG 
CTGATTGCTG CCTGTGATGG AAAAGAGGAC ATCTTTACGT CTATGGTGAA GGAAGTGCTG 
CAACCCAATC AGATCTCTCA GGCCTGGCAG TATGTGAGTC AGCTGCCGGT GCTGGAGATC 
AGTCTGAGTG TGAAGGGATT CTGGGAAAAC TCTGTGGAGC AGACAGAGCG GGCGGTTCCG 
GCTCAGGTGT CCAGCATTAG GGATGAGTCC AGCTGGCTGC CGCTGCATGC GGATCAGGAG 
TACGTCCTGC AGGTGTCCAT GCGCCGGCTC AATGCGGGAC AGCACAGGAG GAAGCAGGAC 
AGCAGAGCTC AGGCGCCGCG TTTCCCCAAA CCCAAAGATG AAGGCTGGTT TCTGGTCCTC 
GGGGAGGTGG AGAAGAAAGA GCTGCTGGCC GTCAAGCGCG TGGGCTTTGT TCGCAACCAC 
AGCAGCGTCT CTGTGGCCTT CTACACTCCC GAGAAGACTG GAAAGTGTAT CTACACGCTG 
TACCTGATGA GCGACAGTTA CCTGGGCCTG GATCAGCAGT ACGACATGCA TCTCAATGTC 
ATCCCGGCCA GCATCGCTGC ACAGGTCAAC AGTGAAGTAG CTGATAGTGT AGGTAAACTG 
GCCCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322471.1
CDS	1..6612
Translation	MSPPRLTGALRSFSNVSKQDDLTQDIYDLKTKRLKRQEIFARDGLTWQKIVQFFTQHLQKKDQPVATQELKNILQAAKQIVGTDFGQDTIESAGVFLFKTFHNKDQIGHEETRAIKHMFGPFPASAAELSCAAVGRLVAQIGESQVEAFIQTQSSLKTPDRGSVFGRNIAFSFDTYVLDAQDDFPWSDGTDMEQTVDFHSFLNNNVGERRVGGVYSAEELEQRNTDGSILRQEVEKYLNEGNMVSSSAEELSTSLFEMLASTRSDDELQNELFEFLGLEGFEMISRLLQRRSDIVDALVSIPADSRHHFTSGKPKGLRDESNMPTYGCQVTIQSEQEKQMMKIYRKEEKKERKRERRGDDGESSESAFHFDPMELRLQREQALLAARSSPVLGRHRDYERIRYPHVYDSYGEATKTSAFIGGSKMLLPEGIRRDNNKMYEEVEIPPNEPMPIGFEEKAVFVSELDEIGQLVFKGMKRLNRIQSIVFETAYNTNENLLICAPTGAGKTNIAMLTILHEIRQHLQPGGVIRKDQFKIVYVAPMKALAAEMTNYFSKRLEPLGIAVKELTGDMQLTKGEILRTQMLVTTPEKWDVVTRKSVGDVALSQVVRLLILDEVHLLHEDRGPVLESLVARTLRQVESTQSMIRILGLSATLPNYLDVATFLHVNPFIGLFYFDSRFRPVPLGQSFVGIKTTNKVQQLHDMEEVCYEKVLKQIKAGHQVMVFVHARNSTVRTAMSLIEMAKNRGELSFFQVDQGADYGQCEKQIQRSRNKQMREMFPDGFGIHHAGMLRQDRSLMESMFSRGYLKVLVCTATLAWGVNLPAHAVIIKGTNIYDAKRGTLVDLGILDVMQIFGRAGRPQFDKYGEGTIITTHDKLSHYLTLLTQQNPIESQFQQSLADNLNAEIALGTVTNVDEAVRWLSYTYLYVRMRANPLAYGINHKAYQMDPQLELYRKELVVESGRKLDKARMIRFDERTGYFASTDLGRTASHFYIKYNTIESFNELFNAQNTEADVLSIVSKAEEFEQIKVRDEELEELEKMLCNFCELPASGGVENGYGKINILLQTYISRGEVDSFSLISDLSYVAQNAARIMRALFEMALRKRWPAMTYRLLNLCKVMDKRLWGWAHPLRQFNTLPASALARMEDKNLTIDKLRDMGKDEIGHMLHHVNIGLKVKQCVHQIPAILLESSIQPITRTVLRVRLSITPDFRWNDQVHGSVGEPWWLWVEDPINDHIYHSEYFLLQKKQVVSGEPQQVVFTIPIFEPMPSQYYIRAVSDRWLGSEAVCIINFQHLILPERHPPHTELLDLQPLPITALGNREYESLYKFTHYNPIQTQIFHTLYHTDTNVLLGAPTGSGKTIAAEMAIFRVFNMYPTSKVVYIAPLKALVRERIEDWKIRIEEKLGRKVVELTGDNTPDMRAIAQADLIVTTPEKWDGVSRSWQNRSYVQKVAILIIDEIHLLGEDRGPVLEVIVSRTNFISSHTSKTVRVVGLSTALANARDLADWLGIGQVGLFNFRPSVRPVPLEVHIQGFPGQHYCPRMATMNKPVFQAIRTHSPAKPVLIFVSSRRQTRLTALDLIAFLATEDDPKQWLHQDEREMTDIIATIRESNLKLTLAFGIGMHHAGLHERDRKTVEELFVNCKIQVLIATSTLAWGVNFPAHLVIVKGTEYYDGKTRRYVDYPITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKFLYEPFPVESSLLSVLSDHLNAEIAAGTVTSKQDAMDYITWTYFFRRLVMNPSYYNLDDISHETINKYLSNLVERSLRDLECSYCMEIQQDEQTIEPLTYGRISSYYYLKHQTIRMFKERLKPELPVHELLAILSEAEEYAELPVRHNEDQLNSELAQRLPLQVNPHSYDSAHTKTHLLLQAHFSRAQLPCSDYGTDTKTVLDNAIRICQAMLDVVANEGWLVSALSLCNLVQMIIQARWLHDSSLLTLPHIQKQELYVFRRWSSRGVRAGCGHQGPIEGLPELIAACDGKEDIFTSMVKEVLQPNQISQAWQYVSQLPVLEISLSVKGFWENSVEQTERAVPAQVSSIRDESSWLPLHADQEYVLQVSMRRLNAGQHRRKQDSRAQAPRFPKPKDEGWFLVLGEVEKKELLAVKRVGFVRNHSSVSVAFYTPEKTGKCIYTLYLMSDSYLGLDQQYDMHLNVIPASIAAQVNSEVADSVGKLALR

Target ORF information:

RefSeq Version	XM_021466796.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio activating signal cointegrator 1 complex subunit 3 (ascc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466796.1

ORF Insert Sequence:

ATGTCTCCTC CTCGGCTCAC TGGAGCCCTG CGCTCCTTCT CTAACGTCAG CAAACAGGAT 
GATTTAACGC AGGACATTTA TGATCTGAAG ACCAAACGCT TAAAACGACA GGAAATATTT 
GCCCGTGATG GTCTGACATG GCAGAAGATA GTTCAGTTCT TCACCCAACA TCTACAGAAG 
AAAGATCAGC CGGTGGCCAC TCAGGAGCTC AAGAACATCC TCCAAGCAGC CAAACAGATC 
GTCGGTACAG ATTTCGGACA AGACACCATT GAAAGTGCCG GGGTCTTCTT GTTTAAAACA 
TTCCACAACA AAGACCAAAT CGGTCATGAG GAAACCCGGG CCATAAAACA CATGTTTGGA 
CCGTTCCCGG CGTCTGCTGC TGAATTGTCC TGTGCAGCGG TGGGCCGTTT AGTGGCTCAG 
ATCGGAGAAT CTCAGGTGGA GGCTTTCATA CAAACGCAGA GTTCCCTCAA GACGCCGGAT 
CGTGGTTCTG TCTTTGGCCG AAACATTGCT TTCTCATTTG ACACCTATGT GCTGGATGCT 
CAAGATGATT TTCCCTGGTC AGATGGGACT GATATGGAAC AGACGGTGGA CTTTCACAGC 
TTTCTCAATA ATAATGTTGG TGAGAGAAGA GTTGGAGGTG TTTACTCTGC AGAAGAACTG 
GAGCAGAGGA ACACAGACGG TTCCATTCTG AGACAGGAGG TGGAGAAATA CCTGAATGAG 
GGAAATATGG TGTCTTCCAG TGCCGAGGAG CTCTCCACCT CTCTTTTCGA AATGCTGGCC 
TCCACAAGAA GCGATGACGA ACTACAAAAC GAGCTCTTTG AGTTTCTGGG TCTTGAGGGG 
TTTGAGATGA TCTCCAGGCT CCTTCAGAGA AGGTCGGACA TTGTTGATGC TTTGGTGTCC 
ATCCCAGCAG ACAGCAGGCA TCACTTCACA TCAGGAAAGC CTAAAGGTCT GCGAGATGAA 
TCCAACATGC CCACATACGG CTGTCAGGTG ACCATCCAGT CTGAGCAGGA GAAACAGATG 
ATGAAGATCT ACAGAAAAGA GGAGAAGAAG GAGAGAAAGC GCGAGAGACG AGGAGATGAT 
GGGGAATCCT CTGAAAGTGC GTTTCATTTT GACCCCATGG AGCTGAGACT GCAGAGGGAG 
CAGGCTTTGC TGGCAGCACG CTCGTCTCCA GTTTTGGGCC GGCACAGAGA TTATGAGCGC 
ATTCGCTACC CTCACGTATA CGATTCTTAC GGCGAAGCCA CCAAAACATC TGCGTTCATC 
GGAGGATCCA AGATGCTGCT GCCCGAGGGG ATCAGAAGAG ACAATAATAA GATGTACGAG 
GAGGTGGAGA TCCCCCCCAA CGAGCCCATG CCCATCGGTT TTGAGGAAAA AGCAGTGTTC 
GTCTCGGAGC TGGACGAGAT CGGCCAGTTG GTGTTCAAGG GCATGAAGAG GCTGAACAGA 
ATCCAGTCTA TAGTGTTCGA GACGGCTTAC AACACCAATG AAAACCTGCT CATATGCGCC 
CCCACCGGTG CCGGAAAAAC CAACATCGCC ATGCTCACAA TACTACACGA GATCCGCCAG 
CACCTACAGC CTGGAGGAGT CATCCGCAAG GACCAGTTTA AGATTGTGTA CGTGGCTCCG 
ATGAAGGCCC TGGCTGCTGA GATGACTAAT TACTTCAGTA AGCGATTGGA GCCTTTGGGC 
ATCGCCGTCA AGGAGCTGAC GGGAGACATG CAGCTGACCA AAGGAGAAAT CCTGCGCACT 
CAGATGTTGG TGACGACTCC AGAGAAGTGG GATGTGGTGA CCAGGAAGAG TGTGGGCGAT 
GTGGCTTTAT CTCAGGTGGT CCGGCTGCTG ATCCTGGATG AAGTTCATCT CCTGCATGAG 
GATAGAGGGC CTGTTCTGGA GAGCCTGGTG GCCCGGACAC TGCGCCAGGT GGAGTCGACC 
CAGAGTATGA TCCGAATACT CGGTCTCTCT GCAACGTTGC CAAATTACCT GGACGTGGCG 
ACTTTCCTCC ATGTGAACCC TTTCATTGGA TTGTTCTACT TCGACAGCCG TTTCCGTCCT 
GTTCCACTTG GCCAGAGCTT CGTTGGCATC AAGACTACAA ATAAGGTCCA ACAGCTGCAC 
GACATGGAGG AGGTTTGTTA TGAAAAAGTC CTGAAGCAGA TTAAAGCGGG TCACCAGGTG 
ATGGTGTTTG TGCATGCCCG AAACTCCACT GTCCGCACCG CCATGAGTCT CATCGAGATG 
GCCAAAAACC GCGGAGAACT GTCCTTCTTT CAGGTCGACC AGGGTGCAGA CTACGGCCAG 
TGTGAGAAAC AGATACAGCG CTCCAGAAAT AAGCAGATGC GAGAGATGTT TCCTGATGGA 
TTCGGGATTC ATCATGCTGG AATGCTGCGT CAGGACCGCT CCCTGATGGA GAGCATGTTT 
TCCAGAGGAT ACCTGAAGGT TCTGGTGTGC ACGGCCACAC TAGCCTGGGG AGTCAACTTA 
CCTGCACATG CCGTCATCAT CAAGGGCACT AATATCTATG ATGCGAAGCG TGGAACTCTG 
GTGGATCTGG GCATCCTGGA TGTGATGCAG ATCTTCGGTC GTGCCGGACG GCCACAGTTT 
GATAAATATG GAGAGGGAAC CATCATCACA ACACACGACA AACTCAGCCA TTACCTGACA 
CTGCTGACCC AGCAGAACCC CATCGAGAGC CAGTTCCAGC AGAGCCTCGC TGACAACCTC 
AACGCTGAGA TCGCTCTCGG GACGGTCACC AATGTCGACG AAGCCGTCCG ATGGTTGAGC 
TACACATACC TGTATGTGCG AATGAGAGCC AATCCACTTG CCTATGGGAT CAACCACAAG 
GCCTATCAGA TGGACCCGCA GCTGGAGCTC TACAGGAAGG AGTTAGTGGT GGAGTCCGGC 
AGGAAGCTGG ATAAAGCACG GATGATCCGA TTCGACGAGA GGACCGGTTA CTTTGCATCT 
ACAGACCTCG GCAGGACAGC CAGTCACTTC TACATTAAAT ACAACACTAT TGAGTCGTTC 
AATGAGCTGT TCAATGCGCA GAACACTGAA GCAGACGTGC TCAGCATCGT CTCCAAAGCT 
GAAGAGTTTG AGCAGATCAA GGTGCGGGAT GAGGAGCTGG AGGAGCTGGA GAAGATGTTG 
TGTAATTTCT GTGAGCTGCC GGCGTCTGGA GGAGTGGAGA ACGGCTACGG CAAGATCAAC 
ATCCTGCTGC AGACCTACAT CAGCCGTGGA GAAGTGGACA GCTTCTCACT CATCTCTGAC 
CTGTCATATG TAGCGCAGAA CGCAGCTCGT ATCATGCGGG CTCTGTTTGA GATGGCTCTG 
AGGAAGCGCT GGCCGGCCAT GACCTACCGC CTGCTGAATC TGTGTAAGGT GATGGACAAG 
CGTCTGTGGG GCTGGGCTCA CCCGCTCAGA CAGTTCAACA CACTTCCGGC TTCAGCGCTG 
GCCCGGATGG AGGACAAGAA CCTCACCATC GACAAACTCA GAGACATGGG CAAAGACGAG 
ATCGGTCACA TGCTGCATCA CGTGAACATC GGTCTGAAGG TGAAGCAGTG TGTTCATCAG 
ATTCCCGCCA TCCTGCTGGA GTCCAGCATT CAGCCAATCA CCCGCACCGT TTTGCGCGTC 
CGCCTCAGCA TCACCCCAGA CTTCAGGTGG AACGACCAGG TCCACGGTTC AGTTGGAGAG 
CCCTGGTGGT TATGGGTAGA GGATCCCATC AATGACCACA TCTACCACTC TGAGTACTTC 
CTGCTGCAGA AGAAACAGGT TGTGAGTGGA GAGCCGCAGC AGGTGGTCTT CACCATCCCC 
ATCTTTGAGC CCATGCCTTC TCAGTATTAC ATCCGCGCCG TGTCTGACCG CTGGCTGGGC 
TCTGAAGCCG TGTGTATCAT CAACTTCCAG CACCTCATTC TGCCAGAAAG ACACCCGCCA 
CATACAGAGC TCTTGGATCT TCAGCCGTTG CCGATTACTG CTTTGGGAAA CAGGGAGTAT 
GAGAGCCTGT ACAAGTTCAC ACACTACAAT CCCATTCAGA CGCAGATCTT CCACACGCTC 
TACCACACAG ACACCAATGT CCTGCTGGGG GCTCCAACCG GCTCCGGAAA AACCATCGCT 
GCAGAGATGG CCATATTCAG GGTCTTCAAC ATGTACCCCA CCTCAAAGGT GGTCTACATT 
GCTCCTCTCA AAGCTCTGGT TCGAGAGAGA ATCGAGGACT GGAAGATCAG AATAGAAGAG 
AAACTTGGGA GAAAGGTGGT TGAGTTAACT GGTGACAATA CTCCGGACAT GCGAGCCATA 
GCACAGGCTG ACCTGATCGT CACGACACCA GAGAAGTGGG ATGGAGTGAG CCGCAGCTGG 
CAGAACCGCA GCTATGTGCA GAAAGTGGCC ATTCTCATCA TTGACGAGAT CCATCTTCTG 
GGTGAAGACC GAGGGCCGGT TCTTGAAGTC ATTGTGTCCA GAACCAACTT TATTTCATCG 
CACACCTCAA AAACGGTCAG AGTAGTAGGA CTGTCCACCG CGCTGGCTAA TGCACGAGAT 
CTAGCCGACT GGCTGGGCAT CGGACAGGTG GGACTGTTTA ATTTCCGGCC CTCTGTTCGC 
CCGGTGCCAT TGGAGGTTCA TATTCAGGGC TTTCCGGGGC AGCATTATTG CCCTAGAATG 
GCCACCATGA ACAAACCAGT ATTCCAAGCA ATCCGTACCC ATTCTCCTGC CAAACCCGTC 
CTGATATTTG TGTCGTCCCG TCGGCAGACG CGGCTCACAG CTCTCGACCT CATTGCCTTC 
CTGGCTACTG AAGATGACCC CAAACAGTGG CTCCACCAGG ACGAGAGAGA GATGACTGAC 
ATCATCGCCA CCATCAGAGA GTCGAACCTG AAGCTGACCC TGGCGTTCGG GATCGGGATG 
CATCATGCAG GCCTTCACGA GCGGGACAGG AAGACGGTGG AAGAACTCTT CGTCAACTGT 
AAGATTCAGG TGTTAATTGC GACGAGCACG CTGGCGTGGG GCGTCAACTT TCCTGCCCAC 
CTGGTGATCG TAAAGGGCAC AGAATACTAC GACGGAAAGA CCAGGCGCTA TGTGGATTAC 
CCCATAACAG ATGTTCTCCA GATGATGGGG CGGGCAGGGC GGCCGCAGTT CGATGACCAG 
GGCAAGGCTG TTATCCTTGT GCACGACATC AAGAAGGACT TTTATAAGAA GTTCCTGTAC 
GAGCCGTTCC CTGTGGAGTC TAGTCTTCTT AGTGTGCTCT CCGACCACTT GAATGCAGAA 
ATCGCAGCAG GCACGGTCAC CTCAAAGCAG GACGCCATGG ATTACATCAC CTGGACGTAT 
TTCTTCCGCA GACTGGTCAT GAACCCTAGC TACTATAATT TGGACGACAT CAGCCATGAA 
ACCATTAATA AATACCTGTC AAACCTGGTG GAGAGGAGTC TGAGGGATCT GGAATGTTCC 
TACTGCATGG AGATACAACA GGACGAGCAA ACCATCGAGC CCCTGACATA CGGCCGCATT 
TCATCCTATT ATTACCTGAA ACACCAGACC ATCCGCATGT TCAAAGAGCG CCTGAAGCCC 
GAGCTGCCGG TCCACGAGTT GCTGGCCATC CTGTCGGAAG CAGAGGAGTA CGCCGAGCTC 
CCAGTACGCC ACAACGAGGA CCAGTTAAAC AGCGAGCTGG CCCAGCGGCT CCCCCTACAG 
GTCAACCCGC ACTCCTATGA CAGCGCACAC ACCAAAACAC ACCTGCTCCT ACAGGCCCAC 
TTCAGCCGCG CACAGCTGCC CTGCTCCGAC TACGGGACCG ACACCAAAAC TGTGCTGGAC 
AACGCCATAC GCATCTGCCA GGCTATGCTG GATGTGGTGG CGAATGAAGG CTGGCTTGTG 
TCCGCGCTGA GTCTCTGTAA TCTGGTCCAG ATGATCATCC AGGCCCGATG GCTGCACGAT 
TCCTCCCTGC TCACACTCCC TCACATCCAG AAGCAGGAGC TCTATGTCTT CAGGAGGTGG 
AGCTCCAGAG GTGTGAGGGC TGGATGTGGC CACCAAGGCC CAATCGAAGG TCTTCCTGAG 
CTGATTGCTG CCTGTGATGG AAAAGAGGAC ATCTTTACGT CTATGGTGAA GGAAGTGCTG 
CAACCCAATC AGATCTCTCA GGCCTGGCAG TATGTGAGTC AGCTGCCGGT GCTGGAGATC 
AGTCTGAGTG TGAAGGGATT CTGGGAAAAC TCTGTGGAGC AGACAGAGCG GGCGGTTCCG 
GCTCAGGTGT CCAGCATTAG GGATGAGTCC AGCTGGCTGC CGCTGCATGC GGATCAGGAG 
TACGTCCTGC AGGTGTCCAT GCGCCGGCTC AATGCGGGAC AGCACAGGAG GAAGCAGGAC 
AGCAGAGCTC AGGCGCCGCG TTTCCCCAAA CCCAAAGATG AAGGCTGGTT TCTGGTCCTC 
GGGGAGGTGG AGAAGAAAGA GCTGCTGGCC GTCAAGCGCG TGGGCTTTGT TCGCAACCAC 
AGCAGCGTCT CTGTGGCCTT CTACACTCCC GAGAAGACTG GAAAGTGTAT CTACACGCTG 
TACCTGATGA GCGACAGTTA CCTGGGCCTG GATCAGCAGT ACGACATGCA TCTCAATGTC 
ATCCCGGCCA GCATCGCTGC ACAGGTCAAC AGTGAAGTAG CTGATAGTGT AGGTAAACTG 
GCCCTGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ascc3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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