MID1 gene cDNA ORF clone, Danio rerio(Zebrafish)

mRNA	Protein	Name
XM_009305010.2	XP_009303285.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_009305009.3	XP_009303284.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_009305010.3	XP_009303285.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_009305011.2	XP_009303286.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_002663432.6	XP_002663478.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_009305009.2	XP_009303284.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_009305012.2	XP_009303287.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
XM_009305012.3	XP_009303287.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
XM_002663432.5	XP_002663478.1	E3 ubiquitin-protein ligase Midline-1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa49417	XM_009305012.3 Latest version!	Danio rerio midline 1 (mid1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa15606	XM_009305010.3 Latest version!	Danio rerio midline 1 (mid1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa15606	XM_009305011.2 Latest version!	Danio rerio midline 1 (mid1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa15606	XM_009305009.3 Latest version!	Danio rerio midline 1 (mid1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa15606	XM_002663432.6 Latest version!	Danio rerio midline 1 (mid1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa15606	XM_009305010.2	Danio rerio midline 1 (mid1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa15606	XM_009305009.2	Danio rerio midline 1 (mid1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa49417	XM_009305012.2	Danio rerio midline 1 (mid1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa15606	XM_002663432.5	Danio rerio midline 1 (mid1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

MID1 ( XM_009305012.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303287.1 Danio rerio midline 1 (mid1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa49417
Clone ID Related Accession (Same CDS sequence)	XM_009305012.2 , XM_009305012.3
Accession Version	XM_009305012.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCGG GACGGCTGCA AATGTTGGAT TCCAACCTCA GCAATTTGAT CAAGAGGAAC 
AGCGAGCTGG AAAACCTGAT GGCAAAGTTG ATCCAGACCT GTCAGCATGT TGAGCACAAT 
GCGGCTCATC AGGAGAGTAA GCTGACGGAG GAGTGCGACA CACTGATCAA CATCGTCCAG 
CAGAGGCGAC AGATCATCGG CACCAAGATC AAAGAGGGAA AGGTGGTTCG CTTGAGAAAG 
CTGGCTCAGC AGATCGCCAA CTGCAAGCAG TTTGTGGAGA GATCGTCCTC GCTCATCATT 
CAAGCCGATC AAGCTCTGAA GGAGACGGAT CACGCTCGAT TCCTGCAGAT GGCCAAGAGC 
ATCTCCGAGA GAGTCTCCAT GGCGACTGCG TCCTCACAGG TCTTGATCCC TGAGATAAAC 
CTGAACGACA CGTTTGACGC GTTCGCATTG GACTTCTCCA GAGAGAAGAA GATGCTGGAA 
GGTCTGGATT ACCTGACAGC TCCGAATGCT CCCACCATCC GCGAGGAGCT GTGCACCGCG 
TCTCACGACA CCATCACGGT GCACTGGACA TCAGAGGACG AGTTCAGTGT GGTTTCATAC 
GAGCTCCAGT ACACCATCTC CACCGGACAG GCCAACGTAG TCAGTCTGTG TAACTCGATG 
GACAGCTGGA TGATCGTGCC CAACATTAAG CAGAATCACT ACACCGTTCA CGGTCTGCAG 
AGCGGCACCA AATACATCTT CATCGTGAAG GCCATGAATC AGGCGGGGAG CCGCAGCAGC 
CCACCGGGAC AACTCAAGAC CAACAGTCAG CCATTTAAAC TGGACCCGAA GTCGGCCCAT 
AGGAAGTTGA AGGTGTCACA TGACAATCTG ACAGTGGAGC GGGACGAAGC GTCCTCCATG 
AAGAGCCACG CACAGGACCG ATTTGGAGGC ACCGGAAACT ACGGTGTGAC TGGAAACGTG 
TACATCGACA GCGGACGCCA CTACTGGGAG GTGCTGATTG GAGGAAGCAC ATGGTTTGCC 
ATCGGCATCG CCTACAAATC AGCCCCCAAA CACGGCTGGG TGGGAAAGGA CTCGACCTCC 
TGGGTGTTGA GCCGATGTAA CACCTCGTGG GCGGTTCGTC ACAACAGTAA AGAGTTTCCC 
ATCGAGCCGT CTCCACACAT GCGGCGTCTG GGCGTCCTGC TGGACTACGA CTCTGGATCT 
CTGGCGTTTT ACGATGCAGC GGGAACGCAA CACCTCTACA CCTTCGACAT CGCCTTCTCA 
CAGCCCATCT GCCCTGTTTT TAGCGTGTGG AATAAGTGTT TGACCGTCCT GACGGGGCTG 
CCCATTCCTG ACCATTTAGA GATCAGTGAC CCGCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303287.1
CDS	36..1397
Translation	MDAGRLQMLDSNLSNLIKRNSELENLMAKLIQTCQHVEHNAAHQESKLTEECDTLINIVQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQFVERSSSLIIQADQALKETDHARFLQMAKSISERVSMATASSQVLIPEINLNDTFDAFALDFSREKKMLEGLDYLTAPNAPTIREELCTASHDTITVHWTSEDEFSVVSYELQYTISTGQANVVSLCNSMDSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAMNQAGSRSSPPGQLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDEASSMKSHAQDRFGGTGNYGVTGNVYIDSGRHYWEVLIGGSTWFAIGIAYKSAPKHGWVGKDSTSWVLSRCNTSWAVRHNSKEFPIEPSPHMRRLGVLLDYDSGSLAFYDAAGTQHLYTFDIAFSQPICPVFSVWNKCLTVLTGLPIPDHLEISDPHD

Target ORF information:

RefSeq Version	XM_009305012.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 1 (mid1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305012.2

ORF Insert Sequence:

ATGGATGCGG GACGGCTGCA AATGTTGGAT TCCAACCTCA GCAATTTGAT CAAGAGGAAC 
AGCGAGCTGG AAAACCTGAT GGCAAAGTTG ATCCAGACCT GTCAGCATGT TGAGCACAAT 
GCGGCTCATC AGGAGAGTAA GCTGACGGAG GAGTGCGACA CACTGATCAA CATCGTCCAG 
CAGAGGCGAC AGATCATCGG CACCAAGATC AAAGAGGGAA AGGTGGTTCG CTTGAGAAAG 
CTGGCTCAGC AGATCGCCAA CTGCAAGCAG TTTGTGGAGA GATCGTCCTC GCTCATCATT 
CAAGCCGATC AAGCTCTGAA GGAGACGGAT CACGCTCGAT TCCTGCAGAT GGCCAAGAGC 
ATCTCCGAGA GAGTCTCCAT GGCGACTGCG TCCTCACAGG TCTTGATCCC TGAGATAAAC 
CTGAACGACA CGTTTGACGC GTTCGCATTG GACTTCTCCA GAGAGAAGAA GATGCTGGAA 
GGTCTGGATT ACCTGACAGC TCCGAATGCT CCCACCATCC GCGAGGAGCT GTGCACCGCG 
TCTCACGACA CCATCACGGT GCACTGGACA TCAGAGGACG AGTTCAGTGT GGTTTCATAC 
GAGCTCCAGT ACACCATCTC CACCGGACAG GCCAACGTAG TCAGTCTGTG TAACTCGATG 
GACAGCTGGA TGATCGTGCC CAACATTAAG CAGAATCACT ACACCGTTCA CGGTCTGCAG 
AGCGGCACCA AATACATCTT CATCGTGAAG GCCATGAATC AGGCGGGGAG CCGCAGCAGC 
CCACCGGGAC AACTCAAGAC CAACAGTCAG CCATTTAAAC TGGACCCGAA GTCGGCCCAT 
AGGAAGTTGA AGGTGTCACA TGACAATCTG ACAGTGGAGC GGGACGAAGC GTCCTCCATG 
AAGAGCCACG CACAGGACCG ATTTGGAGGC ACCGGAAACT ACGGTGTGAC TGGAAACGTG 
TACATCGACA GCGGACGCCA CTACTGGGAG GTGCTGATTG GAGGAAGCAC ATGGTTTGCC 
ATCGGCATCG CCTACAAATC AGCCCCCAAA CACGGCTGGG TGGGAAAGGA CTCGACCTCC 
TGGGTGTTGA GCCGATGTAA CACCTCGTGG GCGGTTCGTC ACAACAGTAA AGAGTTTCCC 
ATCGAGCCGT CTCCACACAT GCGGCGTCTG GGCGTCCTGC TGGACTACGA CTCTGGATCT 
CTGGCGTTTT ACGATGCAGC GGGAACGCAA CACCTCTACA CCTTCGACAT CGCCTTCTCA 
CAGCCCATCT GCCCTGTTTT TAGCGTGTGG AATAAGTGTT TGACCGTCCT GACGGGGCTG 
CCCATTCCTG ACCATTTAGA GATCAGTGAC CCGCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_009305012.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303287.1 Danio rerio midline 1 (mid1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa49417
Clone ID Related Accession (Same CDS sequence)	XM_009305012.2 , XM_009305012.3
Accession Version	XM_009305012.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305012.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCGG GACGGCTGCA AATGTTGGAT TCCAACCTCA GCAATTTGAT CAAGAGGAAC 
AGCGAGCTGG AAAACCTGAT GGCAAAGTTG ATCCAGACCT GTCAGCATGT TGAGCACAAT 
GCGGCTCATC AGGAGAGTAA GCTGACGGAG GAGTGCGACA CACTGATCAA CATCGTCCAG 
CAGAGGCGAC AGATCATCGG CACCAAGATC AAAGAGGGAA AGGTGGTTCG CTTGAGAAAG 
CTGGCTCAGC AGATCGCCAA CTGCAAGCAG TTTGTGGAGA GATCGTCCTC GCTCATCATT 
CAAGCCGATC AAGCTCTGAA GGAGACGGAT CACGCTCGAT TCCTGCAGAT GGCCAAGAGC 
ATCTCCGAGA GAGTCTCCAT GGCGACTGCG TCCTCACAGG TCTTGATCCC TGAGATAAAC 
CTGAACGACA CGTTTGACGC GTTCGCATTG GACTTCTCCA GAGAGAAGAA GATGCTGGAA 
GGTCTGGATT ACCTGACAGC TCCGAATGCT CCCACCATCC GCGAGGAGCT GTGCACCGCG 
TCTCACGACA CCATCACGGT GCACTGGACA TCAGAGGACG AGTTCAGTGT GGTTTCATAC 
GAGCTCCAGT ACACCATCTC CACCGGACAG GCCAACGTAG TCAGTCTGTG TAACTCGATG 
GACAGCTGGA TGATCGTGCC CAACATTAAG CAGAATCACT ACACCGTTCA CGGTCTGCAG 
AGCGGCACCA AATACATCTT CATCGTGAAG GCCATGAATC AGGCGGGGAG CCGCAGCAGC 
CCACCGGGAC AACTCAAGAC CAACAGTCAG CCATTTAAAC TGGACCCGAA GTCGGCCCAT 
AGGAAGTTGA AGGTGTCACA TGACAATCTG ACAGTGGAGC GGGACGAAGC GTCCTCCATG 
AAGAGCCACG CACAGGACCG ATTTGGAGGC ACCGGAAACT ACGGTGTGAC TGGAAACGTG 
TACATCGACA GCGGACGCCA CTACTGGGAG GTGCTGATTG GAGGAAGCAC ATGGTTTGCC 
ATCGGCATCG CCTACAAATC AGCCCCCAAA CACGGCTGGG TGGGAAAGGA CTCGACCTCC 
TGGGTGTTGA GCCGATGTAA CACCTCGTGG GCGGTTCGTC ACAACAGTAA AGAGTTTCCC 
ATCGAGCCGT CTCCACACAT GCGGCGTCTG GGCGTCCTGC TGGACTACGA CTCTGGATCT 
CTGGCGTTTT ACGATGCAGC GGGAACGCAA CACCTCTACA CCTTCGACAT CGCCTTCTCA 
CAGCCCATCT GCCCTGTTTT TAGCGTGTGG AATAAGTGTT TGACCGTCCT GACGGGGCTG 
CCCATTCCTG ACCATTTAGA GATCAGTGAC CCGCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303287.1
CDS	60..1421
Translation	MDAGRLQMLDSNLSNLIKRNSELENLMAKLIQTCQHVEHNAAHQESKLTEECDTLINIVQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQFVERSSSLIIQADQALKETDHARFLQMAKSISERVSMATASSQVLIPEINLNDTFDAFALDFSREKKMLEGLDYLTAPNAPTIREELCTASHDTITVHWTSEDEFSVVSYELQYTISTGQANVVSLCNSMDSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAMNQAGSRSSPPGQLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDEASSMKSHAQDRFGGTGNYGVTGNVYIDSGRHYWEVLIGGSTWFAIGIAYKSAPKHGWVGKDSTSWVLSRCNTSWAVRHNSKEFPIEPSPHMRRLGVLLDYDSGSLAFYDAAGTQHLYTFDIAFSQPICPVFSVWNKCLTVLTGLPIPDHLEISDPHD

Target ORF information:

RefSeq Version	XM_009305012.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 1 (mid1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305012.3

ORF Insert Sequence:

ATGGATGCGG GACGGCTGCA AATGTTGGAT TCCAACCTCA GCAATTTGAT CAAGAGGAAC 
AGCGAGCTGG AAAACCTGAT GGCAAAGTTG ATCCAGACCT GTCAGCATGT TGAGCACAAT 
GCGGCTCATC AGGAGAGTAA GCTGACGGAG GAGTGCGACA CACTGATCAA CATCGTCCAG 
CAGAGGCGAC AGATCATCGG CACCAAGATC AAAGAGGGAA AGGTGGTTCG CTTGAGAAAG 
CTGGCTCAGC AGATCGCCAA CTGCAAGCAG TTTGTGGAGA GATCGTCCTC GCTCATCATT 
CAAGCCGATC AAGCTCTGAA GGAGACGGAT CACGCTCGAT TCCTGCAGAT GGCCAAGAGC 
ATCTCCGAGA GAGTCTCCAT GGCGACTGCG TCCTCACAGG TCTTGATCCC TGAGATAAAC 
CTGAACGACA CGTTTGACGC GTTCGCATTG GACTTCTCCA GAGAGAAGAA GATGCTGGAA 
GGTCTGGATT ACCTGACAGC TCCGAATGCT CCCACCATCC GCGAGGAGCT GTGCACCGCG 
TCTCACGACA CCATCACGGT GCACTGGACA TCAGAGGACG AGTTCAGTGT GGTTTCATAC 
GAGCTCCAGT ACACCATCTC CACCGGACAG GCCAACGTAG TCAGTCTGTG TAACTCGATG 
GACAGCTGGA TGATCGTGCC CAACATTAAG CAGAATCACT ACACCGTTCA CGGTCTGCAG 
AGCGGCACCA AATACATCTT CATCGTGAAG GCCATGAATC AGGCGGGGAG CCGCAGCAGC 
CCACCGGGAC AACTCAAGAC CAACAGTCAG CCATTTAAAC TGGACCCGAA GTCGGCCCAT 
AGGAAGTTGA AGGTGTCACA TGACAATCTG ACAGTGGAGC GGGACGAAGC GTCCTCCATG 
AAGAGCCACG CACAGGACCG ATTTGGAGGC ACCGGAAACT ACGGTGTGAC TGGAAACGTG 
TACATCGACA GCGGACGCCA CTACTGGGAG GTGCTGATTG GAGGAAGCAC ATGGTTTGCC 
ATCGGCATCG CCTACAAATC AGCCCCCAAA CACGGCTGGG TGGGAAAGGA CTCGACCTCC 
TGGGTGTTGA GCCGATGTAA CACCTCGTGG GCGGTTCGTC ACAACAGTAA AGAGTTTCCC 
ATCGAGCCGT CTCCACACAT GCGGCGTCTG GGCGTCCTGC TGGACTACGA CTCTGGATCT 
CTGGCGTTTT ACGATGCAGC GGGAACGCAA CACCTCTACA CCTTCGACAT CGCCTTCTCA 
CAGCCCATCT GCCCTGTTTT TAGCGTGTGG AATAAGTGTT TGACCGTCCT GACGGGGCTG 
CCCATTCCTG ACCATTTAGA GATCAGTGAC CCGCATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_009305010.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303285.1 Danio rerio midline 1 (mid1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa15606
Clone ID Related Accession (Same CDS sequence)	XM_009305010.2 , XM_009305009.3 , XM_009305010.3 , XM_009305011.2 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
Accession Version	XM_009305010.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303285.1
CDS	284..2287
Translation	MDTLESELTCPICLELFEDPLLLPCAHSLCFHCAHRILTSHCAAFDESSKPPSAFQCPTCRYVITLDQQGLDGLKRNVTLQNIIERFQKASLSGPNSPTETRKQISPSAMSSACEAVQCQFCEQDPPQEAVKTCVTCEVSYCQECLRATHPNKKPFTGHRLTEPVPDARLRGLACPEHGEEKVNMYCITDEQLICSLCKLVGHHREHQVASLGERYDKLKQMLDSNLSNLIKRNSELENLMAKLIQTCQHVEHNAAHQESKLTEECDTLINIVQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQFVERSSSLIIQADQALKETDHARFLQMAKSISERVSMATASSQVLIPEINLNDTFDAFALDFSREKKMLEGLDYLTAPNAPTIREELCTASHDTITVHWTSEDEFSVVSYELQYTISTGQANVVSLCNSMDSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAMNQAGSRSSPPGQLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDEASSMKSHAQDRFGGTGNYGVTGNVYIDSGRHYWEVLIGGSTWFAIGIAYKSAPKHGWVGKDSTSWVLSRCNTSWAVRHNSKEFPIEPSPHMRRLGVLLDYDSGSLAFYDAAGTQHLYTFDIAFSQPICPVFSVWNKCLTVLTGLPIPDHLEISDPHD

Target ORF information:

RefSeq Version	XM_009305010.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 1 (mid1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305010.2

ORF Insert Sequence:

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_009305009.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303284.1 Danio rerio midline 1 (mid1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa15606
Clone ID Related Accession (Same CDS sequence)	XM_009305010.2 , XM_009305009.3 , XM_009305010.3 , XM_009305011.2 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
Accession Version	XM_009305009.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303284.1
CDS	147..2150
Translation	MDTLESELTCPICLELFEDPLLLPCAHSLCFHCAHRILTSHCAAFDESSKPPSAFQCPTCRYVITLDQQGLDGLKRNVTLQNIIERFQKASLSGPNSPTETRKQISPSAMSSACEAVQCQFCEQDPPQEAVKTCVTCEVSYCQECLRATHPNKKPFTGHRLTEPVPDARLRGLACPEHGEEKVNMYCITDEQLICSLCKLVGHHREHQVASLGERYDKLKQMLDSNLSNLIKRNSELENLMAKLIQTCQHVEHNAAHQESKLTEECDTLINIVQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQFVERSSSLIIQADQALKETDHARFLQMAKSISERVSMATASSQVLIPEINLNDTFDAFALDFSREKKMLEGLDYLTAPNAPTIREELCTASHDTITVHWTSEDEFSVVSYELQYTISTGQANVVSLCNSMDSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAMNQAGSRSSPPGQLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDEASSMKSHAQDRFGGTGNYGVTGNVYIDSGRHYWEVLIGGSTWFAIGIAYKSAPKHGWVGKDSTSWVLSRCNTSWAVRHNSKEFPIEPSPHMRRLGVLLDYDSGSLAFYDAAGTQHLYTFDIAFSQPICPVFSVWNKCLTVLTGLPIPDHLEISDPHD

Target ORF information:

RefSeq Version	XM_009305009.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 1 (mid1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305009.3

ORF Insert Sequence:

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_009305010.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303285.1 Danio rerio midline 1 (mid1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15606
Clone ID Related Accession (Same CDS sequence)	XM_009305010.2 , XM_009305009.3 , XM_009305010.3 , XM_009305011.2 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
Accession Version	XM_009305010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303285.1
CDS	403..2406
Translation	MDTLESELTCPICLELFEDPLLLPCAHSLCFHCAHRILTSHCAAFDESSKPPSAFQCPTCRYVITLDQQGLDGLKRNVTLQNIIERFQKASLSGPNSPTETRKQISPSAMSSACEAVQCQFCEQDPPQEAVKTCVTCEVSYCQECLRATHPNKKPFTGHRLTEPVPDARLRGLACPEHGEEKVNMYCITDEQLICSLCKLVGHHREHQVASLGERYDKLKQMLDSNLSNLIKRNSELENLMAKLIQTCQHVEHNAAHQESKLTEECDTLINIVQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQFVERSSSLIIQADQALKETDHARFLQMAKSISERVSMATASSQVLIPEINLNDTFDAFALDFSREKKMLEGLDYLTAPNAPTIREELCTASHDTITVHWTSEDEFSVVSYELQYTISTGQANVVSLCNSMDSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAMNQAGSRSSPPGQLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDEASSMKSHAQDRFGGTGNYGVTGNVYIDSGRHYWEVLIGGSTWFAIGIAYKSAPKHGWVGKDSTSWVLSRCNTSWAVRHNSKEFPIEPSPHMRRLGVLLDYDSGSLAFYDAAGTQHLYTFDIAFSQPICPVFSVWNKCLTVLTGLPIPDHLEISDPHD

Target ORF information:

RefSeq Version	XM_009305010.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 1 (mid1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305010.3

ORF Insert Sequence:

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_009305011.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303286.1 Danio rerio midline 1 (mid1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa15606
Clone ID Related Accession (Same CDS sequence)	XM_009305010.2 , XM_009305009.3 , XM_009305010.3 , XM_009305011.2 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
Accession Version	XM_009305011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303286.1
CDS	342..2345
Translation	MDTLESELTCPICLELFEDPLLLPCAHSLCFHCAHRILTSHCAAFDESSKPPSAFQCPTCRYVITLDQQGLDGLKRNVTLQNIIERFQKASLSGPNSPTETRKQISPSAMSSACEAVQCQFCEQDPPQEAVKTCVTCEVSYCQECLRATHPNKKPFTGHRLTEPVPDARLRGLACPEHGEEKVNMYCITDEQLICSLCKLVGHHREHQVASLGERYDKLKQMLDSNLSNLIKRNSELENLMAKLIQTCQHVEHNAAHQESKLTEECDTLINIVQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQFVERSSSLIIQADQALKETDHARFLQMAKSISERVSMATASSQVLIPEINLNDTFDAFALDFSREKKMLEGLDYLTAPNAPTIREELCTASHDTITVHWTSEDEFSVVSYELQYTISTGQANVVSLCNSMDSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAMNQAGSRSSPPGQLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDEASSMKSHAQDRFGGTGNYGVTGNVYIDSGRHYWEVLIGGSTWFAIGIAYKSAPKHGWVGKDSTSWVLSRCNTSWAVRHNSKEFPIEPSPHMRRLGVLLDYDSGSLAFYDAAGTQHLYTFDIAFSQPICPVFSVWNKCLTVLTGLPIPDHLEISDPHD

Target ORF information:

RefSeq Version	XM_009305011.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 1 (mid1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305011.2

ORF Insert Sequence:

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_002663432.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002663478.1 Danio rerio midline 1 (mid1), transcript variant X3, mRNA.

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CloneID	ODa15606
Clone ID Related Accession (Same CDS sequence)	XM_009305010.2 , XM_009305009.3 , XM_009305010.3 , XM_009305011.2 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
Accession Version	XM_002663432.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002663432.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663478.1
CDS	188..2191
Translation	MDTLESELTCPICLELFEDPLLLPCAHSLCFHCAHRILTSHCAAFDESSKPPSAFQCPTCRYVITLDQQGLDGLKRNVTLQNIIERFQKASLSGPNSPTETRKQISPSAMSSACEAVQCQFCEQDPPQEAVKTCVTCEVSYCQECLRATHPNKKPFTGHRLTEPVPDARLRGLACPEHGEEKVNMYCITDEQLICSLCKLVGHHREHQVASLGERYDKLKQMLDSNLSNLIKRNSELENLMAKLIQTCQHVEHNAAHQESKLTEECDTLINIVQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQFVERSSSLIIQADQALKETDHARFLQMAKSISERVSMATASSQVLIPEINLNDTFDAFALDFSREKKMLEGLDYLTAPNAPTIREELCTASHDTITVHWTSEDEFSVVSYELQYTISTGQANVVSLCNSMDSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAMNQAGSRSSPPGQLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDEASSMKSHAQDRFGGTGNYGVTGNVYIDSGRHYWEVLIGGSTWFAIGIAYKSAPKHGWVGKDSTSWVLSRCNTSWAVRHNSKEFPIEPSPHMRRLGVLLDYDSGSLAFYDAAGTQHLYTFDIAFSQPICPVFSVWNKCLTVLTGLPIPDHLEISDPHD

Target ORF information:

RefSeq Version	XM_002663432.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio midline 1 (mid1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663432.6

ORF Insert Sequence:

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_009305009.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009303284.1 Danio rerio midline 1 (mid1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15606
Clone ID Related Accession (Same CDS sequence)	XM_009305010.2 , XM_009305009.3 , XM_009305010.3 , XM_009305011.2 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
Accession Version	XM_009305009.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303284.1
CDS	146..2149
Translation	MDTLESELTCPICLELFEDPLLLPCAHSLCFHCAHRILTSHCAAFDESSKPPSAFQCPTCRYVITLDQQGLDGLKRNVTLQNIIERFQKASLSGPNSPTETRKQISPSAMSSACEAVQCQFCEQDPPQEAVKTCVTCEVSYCQECLRATHPNKKPFTGHRLTEPVPDARLRGLACPEHGEEKVNMYCITDEQLICSLCKLVGHHREHQVASLGERYDKLKQMLDSNLSNLIKRNSELENLMAKLIQTCQHVEHNAAHQESKLTEECDTLINIVQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQFVERSSSLIIQADQALKETDHARFLQMAKSISERVSMATASSQVLIPEINLNDTFDAFALDFSREKKMLEGLDYLTAPNAPTIREELCTASHDTITVHWTSEDEFSVVSYELQYTISTGQANVVSLCNSMDSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAMNQAGSRSSPPGQLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDEASSMKSHAQDRFGGTGNYGVTGNVYIDSGRHYWEVLIGGSTWFAIGIAYKSAPKHGWVGKDSTSWVLSRCNTSWAVRHNSKEFPIEPSPHMRRLGVLLDYDSGSLAFYDAAGTQHLYTFDIAFSQPICPVFSVWNKCLTVLTGLPIPDHLEISDPHD

Target ORF information:

RefSeq Version	XM_009305009.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 1 (mid1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305009.2

ORF Insert Sequence:

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_002663432.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002663478.1 Danio rerio midline 1 (mid1), transcript variant X3, mRNA.

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CloneID	ODa15606
Clone ID Related Accession (Same CDS sequence)	XM_009305010.2 , XM_009305009.3 , XM_009305010.3 , XM_009305011.2 , XM_002663432.6 , XM_009305009.2 , XM_002663432.5
Accession Version	XM_002663432.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002663432.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663478.1
CDS	143..2146
Translation	MDTLESELTCPICLELFEDPLLLPCAHSLCFHCAHRILTSHCAAFDESSKPPSAFQCPTCRYVITLDQQGLDGLKRNVTLQNIIERFQKASLSGPNSPTETRKQISPSAMSSACEAVQCQFCEQDPPQEAVKTCVTCEVSYCQECLRATHPNKKPFTGHRLTEPVPDARLRGLACPEHGEEKVNMYCITDEQLICSLCKLVGHHREHQVASLGERYDKLKQMLDSNLSNLIKRNSELENLMAKLIQTCQHVEHNAAHQESKLTEECDTLINIVQQRRQIIGTKIKEGKVVRLRKLAQQIANCKQFVERSSSLIIQADQALKETDHARFLQMAKSISERVSMATASSQVLIPEINLNDTFDAFALDFSREKKMLEGLDYLTAPNAPTIREELCTASHDTITVHWTSEDEFSVVSYELQYTISTGQANVVSLCNSMDSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAMNQAGSRSSPPGQLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDEASSMKSHAQDRFGGTGNYGVTGNVYIDSGRHYWEVLIGGSTWFAIGIAYKSAPKHGWVGKDSTSWVLSRCNTSWAVRHNSKEFPIEPSPHMRRLGVLLDYDSGSLAFYDAAGTQHLYTFDIAFSQPICPVFSVWNKCLTVLTGLPIPDHLEISDPHD

Target ORF information:

RefSeq Version	XM_002663432.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio midline 1 (mid1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663432.5

ORF Insert Sequence:

ATGGACACAC TGGAGTCTGA GTTGACTTGC CCAATCTGTC TGGAGCTGTT CGAGGATCCG 
CTTCTGCTGC CCTGCGCCCA CAGCCTGTGC TTCCACTGCG CCCACCGCAT CCTGACATCC 
CACTGCGCAG CCTTCGACGA GTCCAGCAAA CCCCCCTCCG CCTTCCAGTG TCCCACCTGC 
CGCTATGTCA TCACGCTGGA CCAGCAGGGT CTAGACGGCC TGAAACGCAA CGTCACTCTA 
CAAAACATCA TCGAGCGCTT CCAGAAGGCC TCTCTGAGCG GGCCGAACTC TCCGACTGAG 
ACACGTAAAC AGATTTCTCC GTCCGCCATG AGTTCTGCAT GCGAGGCCGT ACAGTGTCAG 
TTCTGCGAGC AGGATCCGCC GCAGGAGGCG GTCAAGACTT GCGTCACTTG CGAGGTGTCG 
TATTGTCAGG AGTGTCTTCG GGCGACGCAC CCTAATAAGA AGCCGTTTAC AGGACACAGA 
CTGACAGAGC CAGTCCCGGA TGCAAGACTT AGAGGACTCG CCTGTCCCGA ACATGGAGAG 
GAGAAGGTCA ACATGTACTG CATCACAGAC GAACAGCTCA TTTGTTCGCT GTGTAAACTC 
GTAGGACATC ATCGTGAACA CCAGGTGGCG TCGCTGGGGG AGCGATACGA CAAGCTGAAG 
CAAATGTTGG ATTCCAACCT CAGCAATTTG ATCAAGAGGA ACAGCGAGCT GGAAAACCTG 
ATGGCAAAGT TGATCCAGAC CTGTCAGCAT GTTGAGCACA ATGCGGCTCA TCAGGAGAGT 
AAGCTGACGG AGGAGTGCGA CACACTGATC AACATCGTCC AGCAGAGGCG ACAGATCATC 
GGCACCAAGA TCAAAGAGGG AAAGGTGGTT CGCTTGAGAA AGCTGGCTCA GCAGATCGCC 
AACTGCAAGC AGTTTGTGGA GAGATCGTCC TCGCTCATCA TTCAAGCCGA TCAAGCTCTG 
AAGGAGACGG ATCACGCTCG ATTCCTGCAG ATGGCCAAGA GCATCTCCGA GAGAGTCTCC 
ATGGCGACTG CGTCCTCACA GGTCTTGATC CCTGAGATAA ACCTGAACGA CACGTTTGAC 
GCGTTCGCAT TGGACTTCTC CAGAGAGAAG AAGATGCTGG AAGGTCTGGA TTACCTGACA 
GCTCCGAATG CTCCCACCAT CCGCGAGGAG CTGTGCACCG CGTCTCACGA CACCATCACG 
GTGCACTGGA CATCAGAGGA CGAGTTCAGT GTGGTTTCAT ACGAGCTCCA GTACACCATC 
TCCACCGGAC AGGCCAACGT AGTCAGTCTG TGTAACTCGA TGGACAGCTG GATGATCGTG 
CCCAACATTA AGCAGAATCA CTACACCGTT CACGGTCTGC AGAGCGGCAC CAAATACATC 
TTCATCGTGA AGGCCATGAA TCAGGCGGGG AGCCGCAGCA GCCCACCGGG ACAACTCAAG 
ACCAACAGTC AGCCATTTAA ACTGGACCCG AAGTCGGCCC ATAGGAAGTT GAAGGTGTCA 
CATGACAATC TGACAGTGGA GCGGGACGAA GCGTCCTCCA TGAAGAGCCA CGCACAGGAC 
CGATTTGGAG GCACCGGAAA CTACGGTGTG ACTGGAAACG TGTACATCGA CAGCGGACGC 
CACTACTGGG AGGTGCTGAT TGGAGGAAGC ACATGGTTTG CCATCGGCAT CGCCTACAAA 
TCAGCCCCCA AACACGGCTG GGTGGGAAAG GACTCGACCT CCTGGGTGTT GAGCCGATGT 
AACACCTCGT GGGCGGTTCG TCACAACAGT AAAGAGTTTC CCATCGAGCC GTCTCCACAC 
ATGCGGCGTC TGGGCGTCCT GCTGGACTAC GACTCTGGAT CTCTGGCGTT TTACGATGCA 
GCGGGAACGC AACACCTCTA CACCTTCGAC ATCGCCTTCT CACAGCCCAT CTGCCCTGTT 
TTTAGCGTGT GGAATAAGTG TTTGACCGTC CTGACGGGGC TGCCCATTCC TGACCATTTA 
GAGATCAGTG ACCCGCATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MID1
Entrez Gene ID	100330952
Full Name	midline 1
Synonyms	si:ch1073-168l6.1
General protein information	Preferred Names midline 1 Names midline-1 E3 ubiquitin-protein ligase Midline-1 midline 1 (Opitz/BBB syndrome) trim18
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

Mus musculus (house mouse)	Mid1	NP_034927.2
Homo sapiens (human)	MID1	NP_150632.1
Rattus norvegicus (Norway rat)	Mid1	NP_075216.1
Danio rerio (zebrafish)	mid1	XP_002663478.1
Xenopus tropicalis (tropical clawed frog)	mid1	NP_001006876.1
Bos taurus (cattle)	MID1	NP_001179751.1
Gallus gallus (chicken)	MID1	NP_989460.1
Canis lupus familiaris (dog)	MID1	XP_548857.2
Macaca mulatta (Rhesus monkey)	MID1	XP_001093390.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MID1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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