bub1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bub1
Entrez Gene ID	100307076
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
Synonyms	si:ch1073-192f24.1
General protein information	Preferred Names BUB1 mitotic checkpoint serine/threonine kinase Names BUB1 mitotic checkpoint serine/threonine kinase mitotic checkpoint serine/threonine-protein kinase BUB1 budding uninhibited by benzimidazoles 1-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002665639.5	XP_002665685.2	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
XM_017352663.1	XP_017208152.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
XM_017352662.1	XP_017208151.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_002665639.6	XP_002665685.2	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_021468586.1	XP_021324261.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BUB1	XP_001142040.1
Bos taurus (cattle)	BUB1	NP_001095481.1
Gallus gallus (chicken)	BUB1	NP_001012888.1
Canis lupus familiaris (dog)	BUB1	XP_005630473.1
Homo sapiens (human)	BUB1	NP_004327.1
Rattus norvegicus (Norway rat)	Bub1	NP_001099977.1
Xenopus tropicalis (tropical clawed frog)	bub1	NP_001072830.1
Danio rerio (zebrafish)	bub1	XP_002665685.2
Macaca mulatta (Rhesus monkey)	LOC696598	XP_002808098.1
Mus musculus (house mouse)	Bub1	NP_001106650.1

Gene Ontology

The following bub1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bub1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24598	XM_002665639.6 Latest version!	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa49074	XM_021468586.1 Latest version!	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa56696	XM_017352662.1 Latest version!	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa56697	XM_017352663.1 Latest version!	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa24598	XM_002665639.5	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bub1 ( XM_002665639.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002665685.2 Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa24598
Clone ID Related Accession (Same CDS sequence)	XM_002665639.5 , XM_002665639.6
Accession Version	XM_002665639.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002665639.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATCA ACTCGTATTT TCAGGCCTTT GAGCTGAGCG TACGCAGTTA TGCTGGAGAT 
GATCCGCTCG ACCCCTGGGA CAAGTTTGTA CACTTTCTGG AGAGCAGGAT GTCAGCGGAG 
GAGAAGAAAG GTCTTTCTGT GGTGCTGGAG AGACTAGTTC AGTCCTTTCT TCAAGACGAG 
CGCTACCACA ATGACGTGCG ATACGTCACT CACTGCATCA CCTGTGCAAA CTTATACAGC 
GATCCCATGG AAGTGTACAG TTATTTGCAC AGCCGCGGTG TGGGGACGCA GACGGCCGCA 
CTCTACATTG ACTGGGCTCA GCAGTGCGAG AAGAAAGGGC AGATGGTGCA GGCTGAGATG 
GTGTATAACA GGGCGCTGAT GAACAAGGCT CAGCCGCAGG ACACTGTGCA GGAGCAGTAC 
AGGTTCTTCC AGGAAAGGAC GTCTGCGAGC ACTCCGGCAG CACCGTCAGA TCACGTCCGC 
CATCCTCTTC AAAACTCCAG GATGGTAAAC CAGAACACCC GACCCAGAGA CAATGTTCAG 
CCGCAGTGCA AGGAACCAGA AGCGGCGTGT CCTGCTCCGA GAATGGTCTC CGTCATCTCT 
CGGTCTGAGA ATAACCCGGT GCGGCCAGCC GGCTATACCG CCCCTGAGAC GGTGTCGATG 
TACTGCGTGA AAGAGCTGCA GTGTGAGGGA TCAGAGCTGT CATTTGAAGA GCTGCGAGCC 
CGACGATACT TCGATAAATG CAGACAGCAA GAAGAACAGC GCCGGCTGGC GGAGGAGCAG 
CAGCGGCTCT GGGAAGAGGA AGAGGAGGTG AGGAAGCTTA AGCAGCTGCT GGATGATCTG 
GAGAGGAACA TGAGTCTAAA GCCAGCTGAA CAGACAGACC CTGTGACGCC CGCTGTTATG 
GAGCAGCAGG GCTTAGCCTT TCAGCAGGCT GATCCCCCTG TCACGAGCTA TTCCCCTGCG 
GAGCCGTTTC TCACTGCAGC ACTTGACTCC AGACCTGAGC GAGAGAGTGT CCTGGAGCAG 
CCGGACAGGC CTTCAGAGAT CCAGAGCAGC GTTCCTCAAA GCAGACAAGA GGATGTGTGT 
GTCCAGGGGG AAGCAGACAC ATCGTATGGT GGGTTCAATC ACTCTCATGT CACCCCCAAC 
ACATCTCTGG GGTTCGTTCA GGCCACACCG TCCCGGGTTC TGCCTTCACC CACCGTCAAC 
ACACGCGAGG CTTTGGGTGT GATCATGGAC ATGTTTCAAG CGCCGACGCT CCTCCAGGAG 
TCCATGTTCA ACAGCAGCGT TCAGCCTGAA GACAGCTTCG AGAAGAGCTG CCGGATCAGT 
AGCGCCCCCT CCTGTGTAAA AGCTGCCAGC GCAACTCCGT TCATGATCTA CCAGGACCGA 
GATCAGTCAC AGAGCGCAGG GTCTGATCAG GCTGGAAACA AGCCAGCGGT GACCCGAGCA 
CTGATGGAAA TCCCAGTGTC CAAAGCCAAT GAGACTCCAG TCAGCACAGA GTTCATCACG 
GATGAGAGCA CCATGTGGGG TGCACGAAAC GTCTCTGTGG GCCCATGTCC GAACCATACA 
CGGGATTTCA GCCTGTCTGC TCACCTGGTG TCCACACCGC TGCACCCTCA TGCTCCACAC 
TCGTGGGATG CAGAGCAGAG CCAAGAGGAA AACGCAGTGG TCAGAGGCGT CAGCGCTGCA 
GAGGAAACCC CATACCTCAG ACAGCCCACC AAACTCAGCC CAATCATGGA GCAGAGCCCA 
GCGGAGGAGA AGCCGGCCGA GTGCAGCATG CAGGCTCAGG GCACTATAGT TGGGGAGGGG 
GTGTCTTTAG CACAGCAGAG TCAAGGACTT TCTCTGGCCC AGAGCAGCTG CAGCAGCAGA 
CACCCGCTGG CTGCTTTGTC CTTTCCCGAC CACACGGCCC TCCGAGCACA GGACGAGTCC 
TTGAGGAACA GCAGCAGACC CGCCTGGAGC ATCTATCAGA GCCCAGAGAA GGAGCCCTCG 
CTTCTGGACA CAGCCAAAGA TGAAGACCTA CTGCCCACGA CCTCCTTCCA CAGGCATTCG 
TCAGGGATGA AGCCTTTGGA GACACCGGAT ATTCCAGACA TGAGCCCCAG AGCCGCTCCT 
GGACTCAACT GGTTCCAGAT GGACAGCTCC ACACATGCAG CAGAACCAGA TTTGGATGTC 
ATGATGAGTC CCCCTCCTGT CCCTGGAGCC TGGAACATCT ACCAGAGTCC GGAGAAACCA 
CCCAAAACAG ACTTCCTGTG GGCCATGAGC CCTAAGCCAA TAGCAGAGCA TGCTTTACAC 
ATGGAGGAAG TGGACGTTCT GCTTCCTGAA AGGTCTTTCC TGCAGCGGGA GCCAGGCGTG 
GAAGCACTGC AAGTGTTGCA TGAGTCTCTC CTGATGAGTC CCAAGCGAAC CCCAGCAGCC 
GCTCAGGAGG AGCACTGGAG TCCAAGAGCA GACTGGTCAC ACACTGCCAG CCCACAGCGG 
CCAGTAGAAC AGCATCTGAA TGTCATGACT AGCCCCCCGC AGGGCGCCAG GACCCCCAGA 
GCACCCATGA GCCCCATGGA CACATCCAGA GCAGTGTGTG TTCCTTTGGT GCCGGACCCT 
TGGGACGAGG AGCTGATTGT GTCTCTGCTC TCGAACCTGC AGCCTCCTCT CTCCTCACAG 
CCCAACCTCA CCGTCTGGAG CTGCCACCTG CCCTCCATCA CTCCCAAAAT GACCGTCCAG 
ATCGCTGGAG AGTCTCTGCG TGTGGACTTT GTTCTGGGAC AGGGAGCGTT CGCCACCGTC 
TACCAGGCCA CCAACCTCAT GACCACACAG AAGCTCTTCC TCAAGGTGCA GAAGCCTGCG 
AACCCCTGGG AGTTTTACAT TGACTGTCAG CTGAACAAGC GTCTGCAGCC CAGCGAGAGG 
CATCTCTACA ACAGCATCCA CTCCGCTCAT CTGTTCAACA ACGGCAGCGT CCTCGTCGGA 
CAACTGCACA ACTGCGGCAC ACTGCTGAAC GCTATAAACC TGTACAAGAG GCGCAGTGAG 
AAGCTGATGC CGCAGCCGCT GGTGCTGTAC TTCAGCGTCT GCATCCTGAA GATGGTGGAG 
CTGCTGCATG CGGCACACAT CATCCACGCA GACATCAAGC CCGACAACTT CCTGCTGGGT 
GAGAGGTTTC TGGAGAACGA CTGCTTTGAC CAGGAAAACC TGCAGCATGG CCTGGCTCTC 
ATCGACCTGG GCCAGAGCAT CGACATGACA CTCTTCCCGG AAGGCACTGC GTTCACGGCC 
AAATGCATGA CCTCCGGCTT CCAGTGCGTG GAGATGCTGA GCGGCCGACC CTGGACCTAC 
CAGACCGATT ATTTCGGGAT CGCAGGGACG GTTTACTGTA TGTTGTTTGG GACGTACATG 
GAAGTCAAAC AGGAAGGCGA TGTTTGGAGG ACAAATGGAG TCTTTAAAAG GAACGTTCAC 
GCTGATCTCT GGCAGGATTT CTTCCACACG CTGCTGAACG TGTCGGACTG CAGCGCTCCT 
CCGTGTCTGA GCGTCCTCCG CCAGCGGCTG CACGCCGTCC TGCAGCAGAA CTACAACAGC 
AAGCTGCGCA GCCTGAAGAA CCGGCTGGTG GTGCAGATCC TGGAGGAGGC CCGATCCACA 
CGCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665685.2
CDS	178..3786
Translation	MDINSYFQAFELSVRSYAGDDPLDPWDKFVHFLESRMSAEEKKGLSVVLERLVQSFLQDERYHNDVRYVTHCITCANLYSDPMEVYSYLHSRGVGTQTAALYIDWAQQCEKKGQMVQAEMVYNRALMNKAQPQDTVQEQYRFFQERTSASTPAAPSDHVRHPLQNSRMVNQNTRPRDNVQPQCKEPEAACPAPRMVSVISRSENNPVRPAGYTAPETVSMYCVKELQCEGSELSFEELRARRYFDKCRQQEEQRRLAEEQQRLWEEEEEVRKLKQLLDDLERNMSLKPAEQTDPVTPAVMEQQGLAFQQADPPVTSYSPAEPFLTAALDSRPERESVLEQPDRPSEIQSSVPQSRQEDVCVQGEADTSYGGFNHSHVTPNTSLGFVQATPSRVLPSPTVNTREALGVIMDMFQAPTLLQESMFNSSVQPEDSFEKSCRISSAPSCVKAASATPFMIYQDRDQSQSAGSDQAGNKPAVTRALMEIPVSKANETPVSTEFITDESTMWGARNVSVGPCPNHTRDFSLSAHLVSTPLHPHAPHSWDAEQSQEENAVVRGVSAAEETPYLRQPTKLSPIMEQSPAEEKPAECSMQAQGTIVGEGVSLAQQSQGLSLAQSSCSSRHPLAALSFPDHTALRAQDESLRNSSRPAWSIYQSPEKEPSLLDTAKDEDLLPTTSFHRHSSGMKPLETPDIPDMSPRAAPGLNWFQMDSSTHAAEPDLDVMMSPPPVPGAWNIYQSPEKPPKTDFLWAMSPKPIAEHALHMEEVDVLLPERSFLQREPGVEALQVLHESLLMSPKRTPAAAQEEHWSPRADWSHTASPQRPVEQHLNVMTSPPQGARTPRAPMSPMDTSRAVCVPLVPDPWDEELIVSLLSNLQPPLSSQPNLTVWSCHLPSITPKMTVQIAGESLRVDFVLGQGAFATVYQATNLMTTQKLFLKVQKPANPWEFYIDCQLNKRLQPSERHLYNSIHSAHLFNNGSVLVGQLHNCGTLLNAINLYKRRSEKLMPQPLVLYFSVCILKMVELLHAAHIIHADIKPDNFLLGERFLENDCFDQENLQHGLALIDLGQSIDMTLFPEGTAFTAKCMTSGFQCVEMLSGRPWTYQTDYFGIAGTVYCMLFGTYMEVKQEGDVWRTNGVFKRNVHADLWQDFFHTLLNVSDCSAPPCLSVLRQRLHAVLQQNYNSKLRSLKNRLVVQILEEARSTRR

Target ORF information:

RefSeq Version	XM_002665639.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665639.5

ORF Insert Sequence:

ATGGACATCA ACTCGTATTT TCAGGCCTTT GAGCTGAGCG TACGCAGTTA TGCTGGAGAT 
GATCCGCTCG ACCCCTGGGA CAAGTTTGTA CACTTTCTGG AGAGCAGGAT GTCAGCGGAG 
GAGAAGAAAG GTCTTTCTGT GGTGCTGGAG AGACTAGTTC AGTCCTTTCT TCAAGACGAG 
CGCTACCACA ATGACGTGCG ATACGTCACT CACTGCATCA CCTGTGCAAA CTTATACAGC 
GATCCCATGG AAGTGTACAG TTATTTGCAC AGCCGCGGTG TGGGGACGCA GACGGCCGCA 
CTCTACATTG ACTGGGCTCA GCAGTGCGAG AAGAAAGGGC AGATGGTGCA GGCTGAGATG 
GTGTATAACA GGGCGCTGAT GAACAAGGCT CAGCCGCAGG ACACTGTGCA GGAGCAGTAC 
AGGTTCTTCC AGGAAAGGAC GTCTGCGAGC ACTCCGGCAG CACCGTCAGA TCACGTCCGC 
CATCCTCTTC AAAACTCCAG GATGGTAAAC CAGAACACCC GACCCAGAGA CAATGTTCAG 
CCGCAGTGCA AGGAACCAGA AGCGGCGTGT CCTGCTCCGA GAATGGTCTC CGTCATCTCT 
CGGTCTGAGA ATAACCCGGT GCGGCCAGCC GGCTATACCG CCCCTGAGAC GGTGTCGATG 
TACTGCGTGA AAGAGCTGCA GTGTGAGGGA TCAGAGCTGT CATTTGAAGA GCTGCGAGCC 
CGACGATACT TCGATAAATG CAGACAGCAA GAAGAACAGC GCCGGCTGGC GGAGGAGCAG 
CAGCGGCTCT GGGAAGAGGA AGAGGAGGTG AGGAAGCTTA AGCAGCTGCT GGATGATCTG 
GAGAGGAACA TGAGTCTAAA GCCAGCTGAA CAGACAGACC CTGTGACGCC CGCTGTTATG 
GAGCAGCAGG GCTTAGCCTT TCAGCAGGCT GATCCCCCTG TCACGAGCTA TTCCCCTGCG 
GAGCCGTTTC TCACTGCAGC ACTTGACTCC AGACCTGAGC GAGAGAGTGT CCTGGAGCAG 
CCGGACAGGC CTTCAGAGAT CCAGAGCAGC GTTCCTCAAA GCAGACAAGA GGATGTGTGT 
GTCCAGGGGG AAGCAGACAC ATCGTATGGT GGGTTCAATC ACTCTCATGT CACCCCCAAC 
ACATCTCTGG GGTTCGTTCA GGCCACACCG TCCCGGGTTC TGCCTTCACC CACCGTCAAC 
ACACGCGAGG CTTTGGGTGT GATCATGGAC ATGTTTCAAG CGCCGACGCT CCTCCAGGAG 
TCCATGTTCA ACAGCAGCGT TCAGCCTGAA GACAGCTTCG AGAAGAGCTG CCGGATCAGT 
AGCGCCCCCT CCTGTGTAAA AGCTGCCAGC GCAACTCCGT TCATGATCTA CCAGGACCGA 
GATCAGTCAC AGAGCGCAGG GTCTGATCAG GCTGGAAACA AGCCAGCGGT GACCCGAGCA 
CTGATGGAAA TCCCAGTGTC CAAAGCCAAT GAGACTCCAG TCAGCACAGA GTTCATCACG 
GATGAGAGCA CCATGTGGGG TGCACGAAAC GTCTCTGTGG GCCCATGTCC GAACCATACA 
CGGGATTTCA GCCTGTCTGC TCACCTGGTG TCCACACCGC TGCACCCTCA TGCTCCACAC 
TCGTGGGATG CAGAGCAGAG CCAAGAGGAA AACGCAGTGG TCAGAGGCGT CAGCGCTGCA 
GAGGAAACCC CATACCTCAG ACAGCCCACC AAACTCAGCC CAATCATGGA GCAGAGCCCA 
GCGGAGGAGA AGCCGGCCGA GTGCAGCATG CAGGCTCAGG GCACTATAGT TGGGGAGGGG 
GTGTCTTTAG CACAGCAGAG TCAAGGACTT TCTCTGGCCC AGAGCAGCTG CAGCAGCAGA 
CACCCGCTGG CTGCTTTGTC CTTTCCCGAC CACACGGCCC TCCGAGCACA GGACGAGTCC 
TTGAGGAACA GCAGCAGACC CGCCTGGAGC ATCTATCAGA GCCCAGAGAA GGAGCCCTCG 
CTTCTGGACA CAGCCAAAGA TGAAGACCTA CTGCCCACGA CCTCCTTCCA CAGGCATTCG 
TCAGGGATGA AGCCTTTGGA GACACCGGAT ATTCCAGACA TGAGCCCCAG AGCCGCTCCT 
GGACTCAACT GGTTCCAGAT GGACAGCTCC ACACATGCAG CAGAACCAGA TTTGGATGTC 
ATGATGAGTC CCCCTCCTGT CCCTGGAGCC TGGAACATCT ACCAGAGTCC GGAGAAACCA 
CCCAAAACAG ACTTCCTGTG GGCCATGAGC CCTAAGCCAA TAGCAGAGCA TGCTTTACAC 
ATGGAGGAAG TGGACGTTCT GCTTCCTGAA AGGTCTTTCC TGCAGCGGGA GCCAGGCGTG 
GAAGCACTGC AAGTGTTGCA TGAGTCTCTC CTGATGAGTC CCAAGCGAAC CCCAGCAGCC 
GCTCAGGAGG AGCACTGGAG TCCAAGAGCA GACTGGTCAC ACACTGCCAG CCCACAGCGG 
CCAGTAGAAC AGCATCTGAA TGTCATGACT AGCCCCCCGC AGGGCGCCAG GACCCCCAGA 
GCACCCATGA GCCCCATGGA CACATCCAGA GCAGTGTGTG TTCCTTTGGT GCCGGACCCT 
TGGGACGAGG AGCTGATTGT GTCTCTGCTC TCGAACCTGC AGCCTCCTCT CTCCTCACAG 
CCCAACCTCA CCGTCTGGAG CTGCCACCTG CCCTCCATCA CTCCCAAAAT GACCGTCCAG 
ATCGCTGGAG AGTCTCTGCG TGTGGACTTT GTTCTGGGAC AGGGAGCGTT CGCCACCGTC 
TACCAGGCCA CCAACCTCAT GACCACACAG AAGCTCTTCC TCAAGGTGCA GAAGCCTGCG 
AACCCCTGGG AGTTTTACAT TGACTGTCAG CTGAACAAGC GTCTGCAGCC CAGCGAGAGG 
CATCTCTACA ACAGCATCCA CTCCGCTCAT CTGTTCAACA ACGGCAGCGT CCTCGTCGGA 
CAACTGCACA ACTGCGGCAC ACTGCTGAAC GCTATAAACC TGTACAAGAG GCGCAGTGAG 
AAGCTGATGC CGCAGCCGCT GGTGCTGTAC TTCAGCGTCT GCATCCTGAA GATGGTGGAG 
CTGCTGCATG CGGCACACAT CATCCACGCA GACATCAAGC CCGACAACTT CCTGCTGGGT 
GAGAGGTTTC TGGAGAACGA CTGCTTTGAC CAGGAAAACC TGCAGCATGG CCTGGCTCTC 
ATCGACCTGG GCCAGAGCAT CGACATGACA CTCTTCCCGG AAGGCACTGC GTTCACGGCC 
AAATGCATGA CCTCCGGCTT CCAGTGCGTG GAGATGCTGA GCGGCCGACC CTGGACCTAC 
CAGACCGATT ATTTCGGGAT CGCAGGGACG GTTTACTGTA TGTTGTTTGG GACGTACATG 
GAAGTCAAAC AGGAAGGCGA TGTTTGGAGG ACAAATGGAG TCTTTAAAAG GAACGTTCAC 
GCTGATCTCT GGCAGGATTT CTTCCACACG CTGCTGAACG TGTCGGACTG CAGCGCTCCT 
CCGTGTCTGA GCGTCCTCCG CCAGCGGCTG CACGCCGTCC TGCAGCAGAA CTACAACAGC 
AAGCTGCGCA GCCTGAAGAA CCGGCTGGTG GTGCAGATCC TGGAGGAGGC CCGATCCACA 
CGCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_002665639.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002665685.2 Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24598
Clone ID Related Accession (Same CDS sequence)	XM_002665639.5 , XM_002665639.6
Accession Version	XM_002665639.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002665639.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATCA ACTCGTATTT TCAGGCCTTT GAGCTGAGCG TACGCAGTTA TGCTGGAGAT 
GATCCGCTCG ACCCCTGGGA CAAGTTTGTA CACTTTCTGG AGAGCAGGAT GTCAGCGGAG 
GAGAAGAAAG GTCTTTCTGT GGTGCTGGAG AGACTAGTTC AGTCCTTTCT TCAAGACGAG 
CGCTACCACA ATGACGTGCG ATACGTCACT CACTGCATCA CCTGTGCAAA CTTATACAGC 
GATCCCATGG AAGTGTACAG TTATTTGCAC AGCCGCGGTG TGGGGACGCA GACGGCCGCA 
CTCTACATTG ACTGGGCTCA GCAGTGCGAG AAGAAAGGGC AGATGGTGCA GGCTGAGATG 
GTGTATAACA GGGCGCTGAT GAACAAGGCT CAGCCGCAGG ACACTGTGCA GGAGCAGTAC 
AGGTTCTTCC AGGAAAGGAC GTCTGCGAGC ACTCCGGCAG CACCGTCAGA TCACGTCCGC 
CATCCTCTTC AAAACTCCAG GATGGTAAAC CAGAACACCC GACCCAGAGA CAATGTTCAG 
CCGCAGTGCA AGGAACCAGA AGCGGCGTGT CCTGCTCCGA GAATGGTCTC CGTCATCTCT 
CGGTCTGAGA ATAACCCGGT GCGGCCAGCC GGCTATACCG CCCCTGAGAC GGTGTCGATG 
TACTGCGTGA AAGAGCTGCA GTGTGAGGGA TCAGAGCTGT CATTTGAAGA GCTGCGAGCC 
CGACGATACT TCGATAAATG CAGACAGCAA GAAGAACAGC GCCGGCTGGC GGAGGAGCAG 
CAGCGGCTCT GGGAAGAGGA AGAGGAGGTG AGGAAGCTTA AGCAGCTGCT GGATGATCTG 
GAGAGGAACA TGAGTCTAAA GCCAGCTGAA CAGACAGACC CTGTGACGCC CGCTGTTATG 
GAGCAGCAGG GCTTAGCCTT TCAGCAGGCT GATCCCCCTG TCACGAGCTA TTCCCCTGCG 
GAGCCGTTTC TCACTGCAGC ACTTGACTCC AGACCTGAGC GAGAGAGTGT CCTGGAGCAG 
CCGGACAGGC CTTCAGAGAT CCAGAGCAGC GTTCCTCAAA GCAGACAAGA GGATGTGTGT 
GTCCAGGGGG AAGCAGACAC ATCGTATGGT GGGTTCAATC ACTCTCATGT CACCCCCAAC 
ACATCTCTGG GGTTCGTTCA GGCCACACCG TCCCGGGTTC TGCCTTCACC CACCGTCAAC 
ACACGCGAGG CTTTGGGTGT GATCATGGAC ATGTTTCAAG CGCCGACGCT CCTCCAGGAG 
TCCATGTTCA ACAGCAGCGT TCAGCCTGAA GACAGCTTCG AGAAGAGCTG CCGGATCAGT 
AGCGCCCCCT CCTGTGTAAA AGCTGCCAGC GCAACTCCGT TCATGATCTA CCAGGACCGA 
GATCAGTCAC AGAGCGCAGG GTCTGATCAG GCTGGAAACA AGCCAGCGGT GACCCGAGCA 
CTGATGGAAA TCCCAGTGTC CAAAGCCAAT GAGACTCCAG TCAGCACAGA GTTCATCACG 
GATGAGAGCA CCATGTGGGG TGCACGAAAC GTCTCTGTGG GCCCATGTCC GAACCATACA 
CGGGATTTCA GCCTGTCTGC TCACCTGGTG TCCACACCGC TGCACCCTCA TGCTCCACAC 
TCGTGGGATG CAGAGCAGAG CCAAGAGGAA AACGCAGTGG TCAGAGGCGT CAGCGCTGCA 
GAGGAAACCC CATACCTCAG ACAGCCCACC AAACTCAGCC CAATCATGGA GCAGAGCCCA 
GCGGAGGAGA AGCCGGCCGA GTGCAGCATG CAGGCTCAGG GCACTATAGT TGGGGAGGGG 
GTGTCTTTAG CACAGCAGAG TCAAGGACTT TCTCTGGCCC AGAGCAGCTG CAGCAGCAGA 
CACCCGCTGG CTGCTTTGTC CTTTCCCGAC CACACGGCCC TCCGAGCACA GGACGAGTCC 
TTGAGGAACA GCAGCAGACC CGCCTGGAGC ATCTATCAGA GCCCAGAGAA GGAGCCCTCG 
CTTCTGGACA CAGCCAAAGA TGAAGACCTA CTGCCCACGA CCTCCTTCCA CAGGCATTCG 
TCAGGGATGA AGCCTTTGGA GACACCGGAT ATTCCAGACA TGAGCCCCAG AGCCGCTCCT 
GGACTCAACT GGTTCCAGAT GGACAGCTCC ACACATGCAG CAGAACCAGA TTTGGATGTC 
ATGATGAGTC CCCCTCCTGT CCCTGGAGCC TGGAACATCT ACCAGAGTCC GGAGAAACCA 
CCCAAAACAG ACTTCCTGTG GGCCATGAGC CCTAAGCCAA TAGCAGAGCA TGCTTTACAC 
ATGGAGGAAG TGGACGTTCT GCTTCCTGAA AGGTCTTTCC TGCAGCGGGA GCCAGGCGTG 
GAAGCACTGC AAGTGTTGCA TGAGTCTCTC CTGATGAGTC CCAAGCGAAC CCCAGCAGCC 
GCTCAGGAGG AGCACTGGAG TCCAAGAGCA GACTGGTCAC ACACTGCCAG CCCACAGCGG 
CCAGTAGAAC AGCATCTGAA TGTCATGACT AGCCCCCCGC AGGGCGCCAG GACCCCCAGA 
GCACCCATGA GCCCCATGGA CACATCCAGA GCAGTGTGTG TTCCTTTGGT GCCGGACCCT 
TGGGACGAGG AGCTGATTGT GTCTCTGCTC TCGAACCTGC AGCCTCCTCT CTCCTCACAG 
CCCAACCTCA CCGTCTGGAG CTGCCACCTG CCCTCCATCA CTCCCAAAAT GACCGTCCAG 
ATCGCTGGAG AGTCTCTGCG TGTGGACTTT GTTCTGGGAC AGGGAGCGTT CGCCACCGTC 
TACCAGGCCA CCAACCTCAT GACCACACAG AAGCTCTTCC TCAAGGTGCA GAAGCCTGCG 
AACCCCTGGG AGTTTTACAT TGACTGTCAG CTGAACAAGC GTCTGCAGCC CAGCGAGAGG 
CATCTCTACA ACAGCATCCA CTCCGCTCAT CTGTTCAACA ACGGCAGCGT CCTCGTCGGA 
CAACTGCACA ACTGCGGCAC ACTGCTGAAC GCTATAAACC TGTACAAGAG GCGCAGTGAG 
AAGCTGATGC CGCAGCCGCT GGTGCTGTAC TTCAGCGTCT GCATCCTGAA GATGGTGGAG 
CTGCTGCATG CGGCACACAT CATCCACGCA GACATCAAGC CCGACAACTT CCTGCTGGGT 
GAGAGGTTTC TGGAGAACGA CTGCTTTGAC CAGGAAAACC TGCAGCATGG CCTGGCTCTC 
ATCGACCTGG GCCAGAGCAT CGACATGACA CTCTTCCCGG AAGGCACTGC GTTCACGGCC 
AAATGCATGA CCTCCGGCTT CCAGTGCGTG GAGATGCTGA GCGGCCGACC CTGGACCTAC 
CAGACCGATT ATTTCGGGAT CGCAGGGACG GTTTACTGTA TGTTGTTTGG GACGTACATG 
GAAGTCAAAC AGGAAGGCGA TGTTTGGAGG ACAAATGGAG TCTTTAAAAG GAACGTTCAC 
GCTGATCTCT GGCAGGATTT CTTCCACACG CTGCTGAACG TGTCGGACTG CAGCGCTCCT 
CCGTGTCTGA GCGTCCTCCG CCAGCGGCTG CACGCCGTCC TGCAGCAGAA CTACAACAGC 
AAGCTGCGCA GCCTGAAGAA CCGGCTGGTG GTGCAGATCC TGGAGGAGGC CCGATCCACA 
CGCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665685.2
CDS	188..3796
Translation	MDINSYFQAFELSVRSYAGDDPLDPWDKFVHFLESRMSAEEKKGLSVVLERLVQSFLQDERYHNDVRYVTHCITCANLYSDPMEVYSYLHSRGVGTQTAALYIDWAQQCEKKGQMVQAEMVYNRALMNKAQPQDTVQEQYRFFQERTSASTPAAPSDHVRHPLQNSRMVNQNTRPRDNVQPQCKEPEAACPAPRMVSVISRSENNPVRPAGYTAPETVSMYCVKELQCEGSELSFEELRARRYFDKCRQQEEQRRLAEEQQRLWEEEEEVRKLKQLLDDLERNMSLKPAEQTDPVTPAVMEQQGLAFQQADPPVTSYSPAEPFLTAALDSRPERESVLEQPDRPSEIQSSVPQSRQEDVCVQGEADTSYGGFNHSHVTPNTSLGFVQATPSRVLPSPTVNTREALGVIMDMFQAPTLLQESMFNSSVQPEDSFEKSCRISSAPSCVKAASATPFMIYQDRDQSQSAGSDQAGNKPAVTRALMEIPVSKANETPVSTEFITDESTMWGARNVSVGPCPNHTRDFSLSAHLVSTPLHPHAPHSWDAEQSQEENAVVRGVSAAEETPYLRQPTKLSPIMEQSPAEEKPAECSMQAQGTIVGEGVSLAQQSQGLSLAQSSCSSRHPLAALSFPDHTALRAQDESLRNSSRPAWSIYQSPEKEPSLLDTAKDEDLLPTTSFHRHSSGMKPLETPDIPDMSPRAAPGLNWFQMDSSTHAAEPDLDVMMSPPPVPGAWNIYQSPEKPPKTDFLWAMSPKPIAEHALHMEEVDVLLPERSFLQREPGVEALQVLHESLLMSPKRTPAAAQEEHWSPRADWSHTASPQRPVEQHLNVMTSPPQGARTPRAPMSPMDTSRAVCVPLVPDPWDEELIVSLLSNLQPPLSSQPNLTVWSCHLPSITPKMTVQIAGESLRVDFVLGQGAFATVYQATNLMTTQKLFLKVQKPANPWEFYIDCQLNKRLQPSERHLYNSIHSAHLFNNGSVLVGQLHNCGTLLNAINLYKRRSEKLMPQPLVLYFSVCILKMVELLHAAHIIHADIKPDNFLLGERFLENDCFDQENLQHGLALIDLGQSIDMTLFPEGTAFTAKCMTSGFQCVEMLSGRPWTYQTDYFGIAGTVYCMLFGTYMEVKQEGDVWRTNGVFKRNVHADLWQDFFHTLLNVSDCSAPPCLSVLRQRLHAVLQQNYNSKLRSLKNRLVVQILEEARSTRR

Target ORF information:

RefSeq Version	XM_002665639.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665639.6

ORF Insert Sequence:

ATGGACATCA ACTCGTATTT TCAGGCCTTT GAGCTGAGCG TACGCAGTTA TGCTGGAGAT 
GATCCGCTCG ACCCCTGGGA CAAGTTTGTA CACTTTCTGG AGAGCAGGAT GTCAGCGGAG 
GAGAAGAAAG GTCTTTCTGT GGTGCTGGAG AGACTAGTTC AGTCCTTTCT TCAAGACGAG 
CGCTACCACA ATGACGTGCG ATACGTCACT CACTGCATCA CCTGTGCAAA CTTATACAGC 
GATCCCATGG AAGTGTACAG TTATTTGCAC AGCCGCGGTG TGGGGACGCA GACGGCCGCA 
CTCTACATTG ACTGGGCTCA GCAGTGCGAG AAGAAAGGGC AGATGGTGCA GGCTGAGATG 
GTGTATAACA GGGCGCTGAT GAACAAGGCT CAGCCGCAGG ACACTGTGCA GGAGCAGTAC 
AGGTTCTTCC AGGAAAGGAC GTCTGCGAGC ACTCCGGCAG CACCGTCAGA TCACGTCCGC 
CATCCTCTTC AAAACTCCAG GATGGTAAAC CAGAACACCC GACCCAGAGA CAATGTTCAG 
CCGCAGTGCA AGGAACCAGA AGCGGCGTGT CCTGCTCCGA GAATGGTCTC CGTCATCTCT 
CGGTCTGAGA ATAACCCGGT GCGGCCAGCC GGCTATACCG CCCCTGAGAC GGTGTCGATG 
TACTGCGTGA AAGAGCTGCA GTGTGAGGGA TCAGAGCTGT CATTTGAAGA GCTGCGAGCC 
CGACGATACT TCGATAAATG CAGACAGCAA GAAGAACAGC GCCGGCTGGC GGAGGAGCAG 
CAGCGGCTCT GGGAAGAGGA AGAGGAGGTG AGGAAGCTTA AGCAGCTGCT GGATGATCTG 
GAGAGGAACA TGAGTCTAAA GCCAGCTGAA CAGACAGACC CTGTGACGCC CGCTGTTATG 
GAGCAGCAGG GCTTAGCCTT TCAGCAGGCT GATCCCCCTG TCACGAGCTA TTCCCCTGCG 
GAGCCGTTTC TCACTGCAGC ACTTGACTCC AGACCTGAGC GAGAGAGTGT CCTGGAGCAG 
CCGGACAGGC CTTCAGAGAT CCAGAGCAGC GTTCCTCAAA GCAGACAAGA GGATGTGTGT 
GTCCAGGGGG AAGCAGACAC ATCGTATGGT GGGTTCAATC ACTCTCATGT CACCCCCAAC 
ACATCTCTGG GGTTCGTTCA GGCCACACCG TCCCGGGTTC TGCCTTCACC CACCGTCAAC 
ACACGCGAGG CTTTGGGTGT GATCATGGAC ATGTTTCAAG CGCCGACGCT CCTCCAGGAG 
TCCATGTTCA ACAGCAGCGT TCAGCCTGAA GACAGCTTCG AGAAGAGCTG CCGGATCAGT 
AGCGCCCCCT CCTGTGTAAA AGCTGCCAGC GCAACTCCGT TCATGATCTA CCAGGACCGA 
GATCAGTCAC AGAGCGCAGG GTCTGATCAG GCTGGAAACA AGCCAGCGGT GACCCGAGCA 
CTGATGGAAA TCCCAGTGTC CAAAGCCAAT GAGACTCCAG TCAGCACAGA GTTCATCACG 
GATGAGAGCA CCATGTGGGG TGCACGAAAC GTCTCTGTGG GCCCATGTCC GAACCATACA 
CGGGATTTCA GCCTGTCTGC TCACCTGGTG TCCACACCGC TGCACCCTCA TGCTCCACAC 
TCGTGGGATG CAGAGCAGAG CCAAGAGGAA AACGCAGTGG TCAGAGGCGT CAGCGCTGCA 
GAGGAAACCC CATACCTCAG ACAGCCCACC AAACTCAGCC CAATCATGGA GCAGAGCCCA 
GCGGAGGAGA AGCCGGCCGA GTGCAGCATG CAGGCTCAGG GCACTATAGT TGGGGAGGGG 
GTGTCTTTAG CACAGCAGAG TCAAGGACTT TCTCTGGCCC AGAGCAGCTG CAGCAGCAGA 
CACCCGCTGG CTGCTTTGTC CTTTCCCGAC CACACGGCCC TCCGAGCACA GGACGAGTCC 
TTGAGGAACA GCAGCAGACC CGCCTGGAGC ATCTATCAGA GCCCAGAGAA GGAGCCCTCG 
CTTCTGGACA CAGCCAAAGA TGAAGACCTA CTGCCCACGA CCTCCTTCCA CAGGCATTCG 
TCAGGGATGA AGCCTTTGGA GACACCGGAT ATTCCAGACA TGAGCCCCAG AGCCGCTCCT 
GGACTCAACT GGTTCCAGAT GGACAGCTCC ACACATGCAG CAGAACCAGA TTTGGATGTC 
ATGATGAGTC CCCCTCCTGT CCCTGGAGCC TGGAACATCT ACCAGAGTCC GGAGAAACCA 
CCCAAAACAG ACTTCCTGTG GGCCATGAGC CCTAAGCCAA TAGCAGAGCA TGCTTTACAC 
ATGGAGGAAG TGGACGTTCT GCTTCCTGAA AGGTCTTTCC TGCAGCGGGA GCCAGGCGTG 
GAAGCACTGC AAGTGTTGCA TGAGTCTCTC CTGATGAGTC CCAAGCGAAC CCCAGCAGCC 
GCTCAGGAGG AGCACTGGAG TCCAAGAGCA GACTGGTCAC ACACTGCCAG CCCACAGCGG 
CCAGTAGAAC AGCATCTGAA TGTCATGACT AGCCCCCCGC AGGGCGCCAG GACCCCCAGA 
GCACCCATGA GCCCCATGGA CACATCCAGA GCAGTGTGTG TTCCTTTGGT GCCGGACCCT 
TGGGACGAGG AGCTGATTGT GTCTCTGCTC TCGAACCTGC AGCCTCCTCT CTCCTCACAG 
CCCAACCTCA CCGTCTGGAG CTGCCACCTG CCCTCCATCA CTCCCAAAAT GACCGTCCAG 
ATCGCTGGAG AGTCTCTGCG TGTGGACTTT GTTCTGGGAC AGGGAGCGTT CGCCACCGTC 
TACCAGGCCA CCAACCTCAT GACCACACAG AAGCTCTTCC TCAAGGTGCA GAAGCCTGCG 
AACCCCTGGG AGTTTTACAT TGACTGTCAG CTGAACAAGC GTCTGCAGCC CAGCGAGAGG 
CATCTCTACA ACAGCATCCA CTCCGCTCAT CTGTTCAACA ACGGCAGCGT CCTCGTCGGA 
CAACTGCACA ACTGCGGCAC ACTGCTGAAC GCTATAAACC TGTACAAGAG GCGCAGTGAG 
AAGCTGATGC CGCAGCCGCT GGTGCTGTAC TTCAGCGTCT GCATCCTGAA GATGGTGGAG 
CTGCTGCATG CGGCACACAT CATCCACGCA GACATCAAGC CCGACAACTT CCTGCTGGGT 
GAGAGGTTTC TGGAGAACGA CTGCTTTGAC CAGGAAAACC TGCAGCATGG CCTGGCTCTC 
ATCGACCTGG GCCAGAGCAT CGACATGACA CTCTTCCCGG AAGGCACTGC GTTCACGGCC 
AAATGCATGA CCTCCGGCTT CCAGTGCGTG GAGATGCTGA GCGGCCGACC CTGGACCTAC 
CAGACCGATT ATTTCGGGAT CGCAGGGACG GTTTACTGTA TGTTGTTTGG GACGTACATG 
GAAGTCAAAC AGGAAGGCGA TGTTTGGAGG ACAAATGGAG TCTTTAAAAG GAACGTTCAC 
GCTGATCTCT GGCAGGATTT CTTCCACACG CTGCTGAACG TGTCGGACTG CAGCGCTCCT 
CCGTGTCTGA GCGTCCTCCG CCAGCGGCTG CACGCCGTCC TGCAGCAGAA CTACAACAGC 
AAGCTGCGCA GCCTGAAGAA CCGGCTGGTG GTGCAGATCC TGGAGGAGGC CCGATCCACA 
CGCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_021468586.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324261.1 Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

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CloneID	ODa49074
Clone ID Related Accession (Same CDS sequence)	XM_021468586.1
Accession Version	XM_021468586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATCA ACTCGTATTT TCAGGCCTTT GAGCTGAGCG TACGCAGTTA TGCTGGAGAT 
GATCCGCTCG ACCCCTGGGA CAAGTTTGTA CACTTTCTGG AGAGCAGGAT GTCAGCGGAG 
GAGAAGAAAG GTCTTTCTGT GGTGCTGGAG AGACTAGTTC AGTCCTTTCT TCAAGACGAG 
CGCTACCACA ATGACGTGCG ATACGTCACT CACTGCATCA CCTGTGCAAA CTTATACAGC 
GATCCCATGG AAGTGTACAG TTATTTGCAC AGCCGCGGTG TGGGGACGCA GACGGCCGCA 
CTCTACATTG ACTGGGCTCA GCAGTGCGAG AAGAAAGGGC AGATGGTGCA GGCTGAGATG 
GTGTATAACA GGGCGCTGAT GAACAAGGCT CAGCCGCAGG ACACTGTGCA GGAGCAGTAC 
AGGTTCTTCC AGGAAAGGAC GTCTGCGAGC ACTCCGGCAG CACCGTCAGA TCACGTCCGC 
CATCCTCTTC AAAACTCCAG GATGGTAAAC CAGAACACCC GACCCAGAGA CAATGTTCAG 
CCGCAGTGCA AGGAACCAGA AGCGGCGTGT CCTGCTCCGA GAATGGTCTC CGTCATCTCT 
CGGTCTGAGA ATAACCCGGT GCGGCCAGCC GGCTATACCG CCCCTGAGAC GGTGTCGATG 
TACTGCGTGA AAGAGCTGCA GTGTGAGGGA TCAGAGCTGT CATTTGAAGA GCTGCGAGCC 
CGACGATACT TCGATAAATG CAGACAGCAA GAAGAACAGC GCCGGCTGGC GGAGGAGCAG 
CAGCGGCTCT GGGAAGAGGA AGAGGAGGTG AGGAAGCTTA AGCAGCTGCT GGATGATCTG 
GAGAGGAACA TGAGTCTAAA GCCAGCTGAA CAGACAGACC CTGTGACGCC CGCTGTTATG 
GAGCAGCAGG GCTTAGCCTT TCAGCAGGCT GATCCCCCTG TCACGAGCTA TTCCCCTGCG 
GAGCCGTTTC TCACTGCAGC ACTTGACTCC AGACCTGAGC GAGAGAGTGT CCTGGAGCAG 
CCGGACAGGC CTTCAGAGAT CCAGAGCAGC GTTCCTCAAA GCAGACAAGA GGATGTGTGT 
GTCCAGGGGG AAGCAGACAG TGTGATCATG GACATGTTTC AAGCGCCGAC GCTCCTCCAG 
GAGTCCATGT TCAACAGCAG CGTTCAGCCT GAAGACAGCT TCGAGAAGAG CTGCCGGATC 
AGTAGCGCCC CCTCCTGTGT AAAAGCTGCC AGCGCAACTC CGTTCATGAT CTACCAGGAC 
CGAGATCAGT CACAGAGCGC AGGGTCTGAT CAGGCTGGAA ACAAGCCAGC GGTGACCCGA 
GCACTGATGG AAATCCCAGT GTCCAAAGCC AATGAGACTC CAGTCAGCAC AGAGTTCATC 
ACGGATGAGA GCACCATGTG GGGTGCACGA AACGTCTCTG TGGGCCCATG TCCGAACCAT 
ACACGGGATT TCAGCCTGTC TGCTCACCTG GTGTCCACAC CGCTGCACCC TCATGCTCCA 
CACTCGTGGG ATGCAGAGCA GAGCCAAGAG GAAAACGCAG TGGTCAGAGG CGTCAGCGCT 
GCAGAGGAAA CCCCATACCT CAGACAGCCC ACCAAACTCA GCCCAATCAT GGAGCAGAGC 
CCAGCGGAGG AGAAGCCGGC CGAGTGCAGC ATGCAGGCTC AGGGCACTAT AGTTGGGGAG 
GGGGTGTCTT TAGCACAGCA GAGTCAAGGA CTTTCTCTGG CCCAGAGCAG CTGCAGCAGC 
AGACACCCGC TGGCTGCTTT GTCCTTTCCC GACCACACGG CCCTCCGAGC ACAGGACGAG 
TCCTTGAGGA ACAGCAGCAG ACCCGCCTGG AGCATCTATC AGAGCCCAGA GAAGGAGCCC 
TCGCTTCTGG ACACAGCCAA AGATGAAGAC CTACTGCCCA CGACCTCCTT CCACAGGCAT 
TCGTCAGGGA TGAAGCCTTT GGAGACACCG GATATTCCAG ACATGAGCCC CAGAGCCGCT 
CCTGGACTCA ACTGGTTCCA GATGGACAGC TCCACACATG CAGCAGAACC AGATTTGGAT 
GTCATGATGA GTCCCCCTCC TGTCCCTGGA GCCTGGAACA TCTACCAGAG TCCGGAGAAA 
CCACCCAAAA CAGACTTCCT GTGGGCCATG AGCCCTAAGC CAATAGCAGA GCATGCTTTA 
CACATGGAGG AAGTGGACGT TCTGCTTCCT GAAAGGTCTT TCCTGCAGCG GGAGCCAGGC 
GTGGAAGCAC TGCAAGTGTT GCATGAGTCT CTCCTGATGA GTCCCAAGCG AACCCCAGCA 
GCCGCTCAGG AGGAGCACTG GAGTCCAAGA GCAGACTGGT CACACACTGC CAGCCCACAG 
CGGCCAGTAG AACAGCATCT GAATGTCATG ACTAGCCCCC CGCAGGGCGC CAGGACCCCC 
AGAGCACCCA TGAGCCCCAT GGACACATCC AGAGCAGTGT GTGTTCCTTT GGTGCCGGAC 
CCTTGGGACG AGGAGCTGAT TGTGTCTCTG CTCTCGAACC TGCAGCCTCC TCTCTCCTCA 
CAGCCCAACC TCACCGTCTG GAGCTGCCAC CTGCCCTCCA TCACTCCCAA AATGACCGTC 
CAGATCGCTG GAGAGTCTCT GCGTGTGGAC TTTGTTCTGG GACAGGGAGC GTTCGCCACC 
GTCTACCAGG CCACCAACCT CATGACCACA CAGAAGCTCT TCCTCAAGGT GCAGAAGCCT 
GCGAACCCCT GGGAGTTTTA CATTGACTGT CAGCTGAACA AGCGTCTGCA GCCCAGCGAG 
AGGCATCTCT ACAACAGCAT CCACTCCGCT CATCTGTTCA ACAACGGCAG CGTCCTCGTC 
GGACAACTGC ACAACTGCGG CACACTGCTG AACGCTATAA ACCTGTACAA GAGGCGCAGT 
GAGAAGCTGA TGCCGCAGCC GCTGGTGCTG TACTTCAGCG TCTGCATCCT GAAGATGGTG 
GAGCTGCTGC ATGCGGCACA CATCATCCAC GCAGACATCA AGCCCGACAA CTTCCTGCTG 
GGTGAGAGGT TTCTGGAGAA CGACTGCTTT GACCAGGAAA ACCTGCAGCA TGGCCTGGCT 
CTCATCGACC TGGGCCAGAG CATCGACATG ACACTCTTCC CGGAAGGCAC TGCGTTCACG 
GCCAAATGCA TGACCTCCGG CTTCCAGTGC GTGGAGATGC TGAGCGGCCG ACCCTGGACC 
TACCAGACCG ATTATTTCGG GATCGCAGGG ACGGTTTACT GTATGTTGTT TGGGACGTAC 
ATGGAAGTCA AACAGGAAGG CGATGTTTGG AGGACAAATG GAGTCTTTAA AAGGAACGTT 
CACGCTGATC TCTGGCAGGA TTTCTTCCAC ACGCTGCTGA ACGTGTCGGA CTGCAGCGCT 
CCTCCGTGTC TGAGCGTCCT CCGCCAGCGG CTGCACGCCG TCCTGCAGCA GAACTACAAC 
AGCAAGCTGC GCAGCCTGAA GAACCGGCTG GTGGTGCAGA TCCTGGAGGA GGCCCGATCC 
ACACGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324261.1
CDS	188..3679
Translation	MDINSYFQAFELSVRSYAGDDPLDPWDKFVHFLESRMSAEEKKGLSVVLERLVQSFLQDERYHNDVRYVTHCITCANLYSDPMEVYSYLHSRGVGTQTAALYIDWAQQCEKKGQMVQAEMVYNRALMNKAQPQDTVQEQYRFFQERTSASTPAAPSDHVRHPLQNSRMVNQNTRPRDNVQPQCKEPEAACPAPRMVSVISRSENNPVRPAGYTAPETVSMYCVKELQCEGSELSFEELRARRYFDKCRQQEEQRRLAEEQQRLWEEEEEVRKLKQLLDDLERNMSLKPAEQTDPVTPAVMEQQGLAFQQADPPVTSYSPAEPFLTAALDSRPERESVLEQPDRPSEIQSSVPQSRQEDVCVQGEADSVIMDMFQAPTLLQESMFNSSVQPEDSFEKSCRISSAPSCVKAASATPFMIYQDRDQSQSAGSDQAGNKPAVTRALMEIPVSKANETPVSTEFITDESTMWGARNVSVGPCPNHTRDFSLSAHLVSTPLHPHAPHSWDAEQSQEENAVVRGVSAAEETPYLRQPTKLSPIMEQSPAEEKPAECSMQAQGTIVGEGVSLAQQSQGLSLAQSSCSSRHPLAALSFPDHTALRAQDESLRNSSRPAWSIYQSPEKEPSLLDTAKDEDLLPTTSFHRHSSGMKPLETPDIPDMSPRAAPGLNWFQMDSSTHAAEPDLDVMMSPPPVPGAWNIYQSPEKPPKTDFLWAMSPKPIAEHALHMEEVDVLLPERSFLQREPGVEALQVLHESLLMSPKRTPAAAQEEHWSPRADWSHTASPQRPVEQHLNVMTSPPQGARTPRAPMSPMDTSRAVCVPLVPDPWDEELIVSLLSNLQPPLSSQPNLTVWSCHLPSITPKMTVQIAGESLRVDFVLGQGAFATVYQATNLMTTQKLFLKVQKPANPWEFYIDCQLNKRLQPSERHLYNSIHSAHLFNNGSVLVGQLHNCGTLLNAINLYKRRSEKLMPQPLVLYFSVCILKMVELLHAAHIIHADIKPDNFLLGERFLENDCFDQENLQHGLALIDLGQSIDMTLFPEGTAFTAKCMTSGFQCVEMLSGRPWTYQTDYFGIAGTVYCMLFGTYMEVKQEGDVWRTNGVFKRNVHADLWQDFFHTLLNVSDCSAPPCLSVLRQRLHAVLQQNYNSKLRSLKNRLVVQILEEARSTRR

Target ORF information:

RefSeq Version	XM_021468586.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468586.1

ORF Insert Sequence:

ATGGACATCA ACTCGTATTT TCAGGCCTTT GAGCTGAGCG TACGCAGTTA TGCTGGAGAT 
GATCCGCTCG ACCCCTGGGA CAAGTTTGTA CACTTTCTGG AGAGCAGGAT GTCAGCGGAG 
GAGAAGAAAG GTCTTTCTGT GGTGCTGGAG AGACTAGTTC AGTCCTTTCT TCAAGACGAG 
CGCTACCACA ATGACGTGCG ATACGTCACT CACTGCATCA CCTGTGCAAA CTTATACAGC 
GATCCCATGG AAGTGTACAG TTATTTGCAC AGCCGCGGTG TGGGGACGCA GACGGCCGCA 
CTCTACATTG ACTGGGCTCA GCAGTGCGAG AAGAAAGGGC AGATGGTGCA GGCTGAGATG 
GTGTATAACA GGGCGCTGAT GAACAAGGCT CAGCCGCAGG ACACTGTGCA GGAGCAGTAC 
AGGTTCTTCC AGGAAAGGAC GTCTGCGAGC ACTCCGGCAG CACCGTCAGA TCACGTCCGC 
CATCCTCTTC AAAACTCCAG GATGGTAAAC CAGAACACCC GACCCAGAGA CAATGTTCAG 
CCGCAGTGCA AGGAACCAGA AGCGGCGTGT CCTGCTCCGA GAATGGTCTC CGTCATCTCT 
CGGTCTGAGA ATAACCCGGT GCGGCCAGCC GGCTATACCG CCCCTGAGAC GGTGTCGATG 
TACTGCGTGA AAGAGCTGCA GTGTGAGGGA TCAGAGCTGT CATTTGAAGA GCTGCGAGCC 
CGACGATACT TCGATAAATG CAGACAGCAA GAAGAACAGC GCCGGCTGGC GGAGGAGCAG 
CAGCGGCTCT GGGAAGAGGA AGAGGAGGTG AGGAAGCTTA AGCAGCTGCT GGATGATCTG 
GAGAGGAACA TGAGTCTAAA GCCAGCTGAA CAGACAGACC CTGTGACGCC CGCTGTTATG 
GAGCAGCAGG GCTTAGCCTT TCAGCAGGCT GATCCCCCTG TCACGAGCTA TTCCCCTGCG 
GAGCCGTTTC TCACTGCAGC ACTTGACTCC AGACCTGAGC GAGAGAGTGT CCTGGAGCAG 
CCGGACAGGC CTTCAGAGAT CCAGAGCAGC GTTCCTCAAA GCAGACAAGA GGATGTGTGT 
GTCCAGGGGG AAGCAGACAG TGTGATCATG GACATGTTTC AAGCGCCGAC GCTCCTCCAG 
GAGTCCATGT TCAACAGCAG CGTTCAGCCT GAAGACAGCT TCGAGAAGAG CTGCCGGATC 
AGTAGCGCCC CCTCCTGTGT AAAAGCTGCC AGCGCAACTC CGTTCATGAT CTACCAGGAC 
CGAGATCAGT CACAGAGCGC AGGGTCTGAT CAGGCTGGAA ACAAGCCAGC GGTGACCCGA 
GCACTGATGG AAATCCCAGT GTCCAAAGCC AATGAGACTC CAGTCAGCAC AGAGTTCATC 
ACGGATGAGA GCACCATGTG GGGTGCACGA AACGTCTCTG TGGGCCCATG TCCGAACCAT 
ACACGGGATT TCAGCCTGTC TGCTCACCTG GTGTCCACAC CGCTGCACCC TCATGCTCCA 
CACTCGTGGG ATGCAGAGCA GAGCCAAGAG GAAAACGCAG TGGTCAGAGG CGTCAGCGCT 
GCAGAGGAAA CCCCATACCT CAGACAGCCC ACCAAACTCA GCCCAATCAT GGAGCAGAGC 
CCAGCGGAGG AGAAGCCGGC CGAGTGCAGC ATGCAGGCTC AGGGCACTAT AGTTGGGGAG 
GGGGTGTCTT TAGCACAGCA GAGTCAAGGA CTTTCTCTGG CCCAGAGCAG CTGCAGCAGC 
AGACACCCGC TGGCTGCTTT GTCCTTTCCC GACCACACGG CCCTCCGAGC ACAGGACGAG 
TCCTTGAGGA ACAGCAGCAG ACCCGCCTGG AGCATCTATC AGAGCCCAGA GAAGGAGCCC 
TCGCTTCTGG ACACAGCCAA AGATGAAGAC CTACTGCCCA CGACCTCCTT CCACAGGCAT 
TCGTCAGGGA TGAAGCCTTT GGAGACACCG GATATTCCAG ACATGAGCCC CAGAGCCGCT 
CCTGGACTCA ACTGGTTCCA GATGGACAGC TCCACACATG CAGCAGAACC AGATTTGGAT 
GTCATGATGA GTCCCCCTCC TGTCCCTGGA GCCTGGAACA TCTACCAGAG TCCGGAGAAA 
CCACCCAAAA CAGACTTCCT GTGGGCCATG AGCCCTAAGC CAATAGCAGA GCATGCTTTA 
CACATGGAGG AAGTGGACGT TCTGCTTCCT GAAAGGTCTT TCCTGCAGCG GGAGCCAGGC 
GTGGAAGCAC TGCAAGTGTT GCATGAGTCT CTCCTGATGA GTCCCAAGCG AACCCCAGCA 
GCCGCTCAGG AGGAGCACTG GAGTCCAAGA GCAGACTGGT CACACACTGC CAGCCCACAG 
CGGCCAGTAG AACAGCATCT GAATGTCATG ACTAGCCCCC CGCAGGGCGC CAGGACCCCC 
AGAGCACCCA TGAGCCCCAT GGACACATCC AGAGCAGTGT GTGTTCCTTT GGTGCCGGAC 
CCTTGGGACG AGGAGCTGAT TGTGTCTCTG CTCTCGAACC TGCAGCCTCC TCTCTCCTCA 
CAGCCCAACC TCACCGTCTG GAGCTGCCAC CTGCCCTCCA TCACTCCCAA AATGACCGTC 
CAGATCGCTG GAGAGTCTCT GCGTGTGGAC TTTGTTCTGG GACAGGGAGC GTTCGCCACC 
GTCTACCAGG CCACCAACCT CATGACCACA CAGAAGCTCT TCCTCAAGGT GCAGAAGCCT 
GCGAACCCCT GGGAGTTTTA CATTGACTGT CAGCTGAACA AGCGTCTGCA GCCCAGCGAG 
AGGCATCTCT ACAACAGCAT CCACTCCGCT CATCTGTTCA ACAACGGCAG CGTCCTCGTC 
GGACAACTGC ACAACTGCGG CACACTGCTG AACGCTATAA ACCTGTACAA GAGGCGCAGT 
GAGAAGCTGA TGCCGCAGCC GCTGGTGCTG TACTTCAGCG TCTGCATCCT GAAGATGGTG 
GAGCTGCTGC ATGCGGCACA CATCATCCAC GCAGACATCA AGCCCGACAA CTTCCTGCTG 
GGTGAGAGGT TTCTGGAGAA CGACTGCTTT GACCAGGAAA ACCTGCAGCA TGGCCTGGCT 
CTCATCGACC TGGGCCAGAG CATCGACATG ACACTCTTCC CGGAAGGCAC TGCGTTCACG 
GCCAAATGCA TGACCTCCGG CTTCCAGTGC GTGGAGATGC TGAGCGGCCG ACCCTGGACC 
TACCAGACCG ATTATTTCGG GATCGCAGGG ACGGTTTACT GTATGTTGTT TGGGACGTAC 
ATGGAAGTCA AACAGGAAGG CGATGTTTGG AGGACAAATG GAGTCTTTAA AAGGAACGTT 
CACGCTGATC TCTGGCAGGA TTTCTTCCAC ACGCTGCTGA ACGTGTCGGA CTGCAGCGCT 
CCTCCGTGTC TGAGCGTCCT CCGCCAGCGG CTGCACGCCG TCCTGCAGCA GAACTACAAC 
AGCAAGCTGC GCAGCCTGAA GAACCGGCTG GTGGTGCAGA TCCTGGAGGA GGCCCGATCC 
ACACGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_017352662.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208151.1 Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56696
Clone ID Related Accession (Same CDS sequence)	XM_017352662.1
Accession Version	XM_017352662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGCA TAATGGTCTT AATAAGGTCT TTAACTGTGT TTAAATTGAT GATGATGTAT 
GTGTGTGACT TCAGTAAGCC TTTTAAAAGC TTATACTTGT GTGTCTTCAG TCATGATGTG 
TGTGTGTTTG TTCATCGTCA CTGCAGGGCC TTTGAGCTGA GCGTACGCAG TTATGCTGGA 
GATGATCCGC TCGACCCCTG GGACAAGTTT GTACACTTTC TGGAGAGCAG GATGTCAGCG 
GAGGAGAAGA AAGGTCTTTC TGTGGTGCTG GAGAGACTAG TTCAGTCCTT TCTTCAAGAC 
GAGCGCTACC ACAATGACGT GCGATACGTC ACTCACTGCA TCACCTGTGC AAACTTATAC 
AGCGATCCCA TGGAAGTGTA CAGTTATTTG CACAGCCGCG GTGTGGGGAC GCAGACGGCC 
GCACTCTACA TTGACTGGGC TCAGCAGTGC GAGAAGAAAG GGCAGATGGT GCAGGCTGAG 
ATGGTGTATA ACAGGGCGCT GATGAACAAG GCTCAGCCGC AGGACACTGT GCAGGAGCAG 
TACAGGTTCT TCCAGGAAAG GACGTCTGCG AGCACTCCGG CAGCACCGTC AGATCACGTC 
CGCCATCCTC TTCAAAACTC CAGGATGGTA AACCAGAACA CCCGACCCAG AGACAATGTT 
CAGCCGCAGT GCAAGGAACC AGAAGCGGCG TGTCCTGCTC CGAGAATGGT CTCCGTCATC 
TCTCGGTCTG AGAATAACCC GGTGCGGCCA GCCGGCTATA CCGCCCCTGA GACGGTGTCG 
ATGTACTGCG TGAAAGAGCT GCAGTGTGAG GGATCAGAGC TGTCATTTGA AGAGCTGCGA 
GCCCGACGAT ACTTCGATAA ATGCAGACAG CAAGAAGAAC AGCGCCGGCT GGCGGAGGAG 
CAGCAGCGGC TCTGGGAAGA GGAAGAGGAG GTGAGGAAGC TTAAGCAGCT GCTGGATGAT 
CTGGAGAGGA ACATGAGTCT AAAGCCAGCT GAACAGACAG ACCCTGTGAC GCCCGCTGTT 
ATGGAGCAGC AGGGCTTAGC CTTTCAGCAG GCTGATCCCC CTGTCACGAG CTATTCCCCT 
GCGGAGCCGT TTCTCACTGC AGCACTTGAC TCCAGACCTG AGCGAGAGAG TGTCCTGGAG 
CAGCCGGACA GGCCTTCAGA GATCCAGAGC AGCGTTCCTC AAAGCAGACA AGAGGATGTG 
TGTGTCCAGG GGGAAGCAGA CACATCGTAT GGTGGGTTCA ATCACTCTCA TGTCACCCCC 
AACACATCTC TGGGGTTCGT TCAGGCCACA CCGTCCCGGG TTCTGCCTTC ACCCACCGTC 
AACACACGCG AGGCTTTGGG TGTGATCATG GACATGTTTC AAGCGCCGAC GCTCCTCCAG 
GAGTCCATGT TCAACAGCAG CGTTCAGCCT GAAGACAGCT TCGAGAAGAG CTGCCGGATC 
AGTAGCGCCC CCTCCTGTGT AAAAGCTGCC AGCGCAACTC CGTTCATGAT CTACCAGGAC 
CGAGATCAGT CACAGAGCGC AGGGTCTGAT CAGGCTGGAA ACAAGCCAGC GGTGACCCGA 
GCACTGATGG AAATCCCAGT GTCCAAAGCC AATGAGACTC CAGTCAGCAC AGAGTTCATC 
ACGGATGAGA GCACCATGTG GGGTGCACGA AACGTCTCTG TGGGCCCATG TCCGAACCAT 
ACACGGGATT TCAGCCTGTC TGCTCACCTG GTGTCCACAC CGCTGCACCC TCATGCTCCA 
CACTCGTGGG ATGCAGAGCA GAGCCAAGAG GAAAACGCAG TGGTCAGAGG CGTCAGCGCT 
GCAGAGGAAA CCCCATACCT CAGACAGCCC ACCAAACTCA GCCCAATCAT GGAGCAGAGC 
CCAGCGGAGG AGAAGCCGGC CGAGTGCAGC ATGCAGGCTC AGGGCACTAT AGTTGGGGAG 
GGGGTGTCTT TAGCACAGCA GAGTCAAGGA CTTTCTCTGG CCCAGAGCAG CTGCAGCAGC 
AGACACCCGC TGGCTGCTTT GTCCTTTCCC GACCACACGG CCCTCCGAGC ACAGGACGAG 
TCCTTGAGGA ACAGCAGCAG ACCCGCCTGG AGCATCTATC AGAGCCCAGA GAAGGAGCCC 
TCGCTTCTGG ACACAGCCAA AGATGAAGAC CTACTGCCCA CGACCTCCTT CCACAGGCAT 
TCGTCAGGGA TGAAGCCTTT GGAGACACCG GATATTCCAG ACATGAGCCC CAGAGCCGCT 
CCTGGACTCA ACTGGTTCCA GATGGACAGC TCCACACATG CAGCAGAACC AGATTTGGAT 
GTCATGATGA GTCCCCCTCC TGTCCCTGGA GCCTGGAACA TCTACCAGAG TCCGGAGAAA 
CCACCCAAAA CAGACTTCCT GTGGGCCATG AGCCCTAAGC CAATAGCAGA GCATGCTTTA 
CACATGGAGG AAGTGGACGT TCTGCTTCCT GAAAGGTCTT TCCTGCAGCG GGAGCCAGGC 
GTGGAAGCAC TGCAAGTGTT GCATGAGTCT CTCCTGATGA GTCCCAAGCG AACCCCAGCA 
GCCGCTCAGG AGGAGCACTG GAGTCCAAGA GCAGACTGGT CACACACTGC CAGCCCACAG 
CGGCCAGTAG AACAGCATCT GAATGTCATG ACTAGCCCCC CGCAGGGCGC CAGGACCCCC 
AGAGCACCCA TGAGCCCCAT GGACACATCC AGAGCAGTGT GTGTTCCTTT GGTGCCGGAC 
CCTTGGGACG AGGAGCTGAT TGTGTCTCTG CTCTCGAACC TGCAGCCTCC TCTCTCCTCA 
CAGCCCAACC TCACCGTCTG GAGCTGCCAC CTGCCCTCCA TCACTCCCAA AATGACCGTC 
CAGATCGCTG GAGAGTCTCT GCGTGTGGAC TTTGTTCTGG GACAGGGAGC GTTCGCCACC 
GTCTACCAGG CCACCAACCT CATGACCACA CAGAAGCTCT TCCTCAAGGT GCAGAAGCCT 
GCGAACCCCT GGGAGTTTTA CATTGACTGT CAGCTGAACA AGCGTCTGCA GCCCAGCGAG 
AGGCATCTCT ACAACAGCAT CCACTCCGCT CATCTGTTCA ACAACGGCAG CGTCCTCGTC 
GGACAACTGC ACAACTGCGG CACACTGCTG AACGCTATAA ACCTGTACAA GAGGCGCAGT 
GAGAAGCTGA TGCCGCAGCC GCTGGTGCTG TACTTCAGCG TCTGCATCCT GAAGATGGTG 
GAGCTGCTGC ATGCGGCACA CATCATCCAC GCAGACATCA AGCCCGACAA CTTCCTGCTG 
GGTGAGAGGT TTCTGGAGAA CGACTGCTTT GACCAGGAAA ACCTGCAGCA TGGCCTGGCT 
CTCATCGACC TGGGCCAGAG CATCGACATG ACACTCTTCC CGGAAGGCAC TGCGTTCACG 
GCCAAATGCA TGACCTCCGG CTTCCAGTGC GTGGAGATGC TGAGCGGCCG ACCCTGGACC 
TACCAGACCG ATTATTTCGG GATCGCAGGG ACGGTTTACT GTATGTTGTT TGGGACGTAC 
ATGGAAGTCA AACAGGAAGG CGATGTTTGG AGGACAAATG GAGTCTTTAA AAGGAACGTT 
CACGCTGATC TCTGGCAGGA TTTCTTCCAC ACGCTGCTGA ACGTGTCGGA CTGCAGCGCT 
CCTCCGTGTC TGAGCGTCCT CCGCCAGCGG CTGCACGCCG TCCTGCAGCA GAACTACAAC 
AGCAAGCTGC GCAGCCTGAA GAACCGGCTG GTGGTGCAGA TCCTGGAGGA GGCCCGATCC 
ACACGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208151.1
CDS	200..3931
Translation	MSCIMVLIRSLTVFKLMMMYVCDFSKPFKSLYLCVFSHDVCVFVHRHCRAFELSVRSYAGDDPLDPWDKFVHFLESRMSAEEKKGLSVVLERLVQSFLQDERYHNDVRYVTHCITCANLYSDPMEVYSYLHSRGVGTQTAALYIDWAQQCEKKGQMVQAEMVYNRALMNKAQPQDTVQEQYRFFQERTSASTPAAPSDHVRHPLQNSRMVNQNTRPRDNVQPQCKEPEAACPAPRMVSVISRSENNPVRPAGYTAPETVSMYCVKELQCEGSELSFEELRARRYFDKCRQQEEQRRLAEEQQRLWEEEEEVRKLKQLLDDLERNMSLKPAEQTDPVTPAVMEQQGLAFQQADPPVTSYSPAEPFLTAALDSRPERESVLEQPDRPSEIQSSVPQSRQEDVCVQGEADTSYGGFNHSHVTPNTSLGFVQATPSRVLPSPTVNTREALGVIMDMFQAPTLLQESMFNSSVQPEDSFEKSCRISSAPSCVKAASATPFMIYQDRDQSQSAGSDQAGNKPAVTRALMEIPVSKANETPVSTEFITDESTMWGARNVSVGPCPNHTRDFSLSAHLVSTPLHPHAPHSWDAEQSQEENAVVRGVSAAEETPYLRQPTKLSPIMEQSPAEEKPAECSMQAQGTIVGEGVSLAQQSQGLSLAQSSCSSRHPLAALSFPDHTALRAQDESLRNSSRPAWSIYQSPEKEPSLLDTAKDEDLLPTTSFHRHSSGMKPLETPDIPDMSPRAAPGLNWFQMDSSTHAAEPDLDVMMSPPPVPGAWNIYQSPEKPPKTDFLWAMSPKPIAEHALHMEEVDVLLPERSFLQREPGVEALQVLHESLLMSPKRTPAAAQEEHWSPRADWSHTASPQRPVEQHLNVMTSPPQGARTPRAPMSPMDTSRAVCVPLVPDPWDEELIVSLLSNLQPPLSSQPNLTVWSCHLPSITPKMTVQIAGESLRVDFVLGQGAFATVYQATNLMTTQKLFLKVQKPANPWEFYIDCQLNKRLQPSERHLYNSIHSAHLFNNGSVLVGQLHNCGTLLNAINLYKRRSEKLMPQPLVLYFSVCILKMVELLHAAHIIHADIKPDNFLLGERFLENDCFDQENLQHGLALIDLGQSIDMTLFPEGTAFTAKCMTSGFQCVEMLSGRPWTYQTDYFGIAGTVYCMLFGTYMEVKQEGDVWRTNGVFKRNVHADLWQDFFHTLLNVSDCSAPPCLSVLRQRLHAVLQQNYNSKLRSLKNRLVVQILEEARSTRR

Target ORF information:

RefSeq Version	XM_017352662.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352662.1

ORF Insert Sequence:

ATGTCATGCA TAATGGTCTT AATAAGGTCT TTAACTGTGT TTAAATTGAT GATGATGTAT 
GTGTGTGACT TCAGTAAGCC TTTTAAAAGC TTATACTTGT GTGTCTTCAG TCATGATGTG 
TGTGTGTTTG TTCATCGTCA CTGCAGGGCC TTTGAGCTGA GCGTACGCAG TTATGCTGGA 
GATGATCCGC TCGACCCCTG GGACAAGTTT GTACACTTTC TGGAGAGCAG GATGTCAGCG 
GAGGAGAAGA AAGGTCTTTC TGTGGTGCTG GAGAGACTAG TTCAGTCCTT TCTTCAAGAC 
GAGCGCTACC ACAATGACGT GCGATACGTC ACTCACTGCA TCACCTGTGC AAACTTATAC 
AGCGATCCCA TGGAAGTGTA CAGTTATTTG CACAGCCGCG GTGTGGGGAC GCAGACGGCC 
GCACTCTACA TTGACTGGGC TCAGCAGTGC GAGAAGAAAG GGCAGATGGT GCAGGCTGAG 
ATGGTGTATA ACAGGGCGCT GATGAACAAG GCTCAGCCGC AGGACACTGT GCAGGAGCAG 
TACAGGTTCT TCCAGGAAAG GACGTCTGCG AGCACTCCGG CAGCACCGTC AGATCACGTC 
CGCCATCCTC TTCAAAACTC CAGGATGGTA AACCAGAACA CCCGACCCAG AGACAATGTT 
CAGCCGCAGT GCAAGGAACC AGAAGCGGCG TGTCCTGCTC CGAGAATGGT CTCCGTCATC 
TCTCGGTCTG AGAATAACCC GGTGCGGCCA GCCGGCTATA CCGCCCCTGA GACGGTGTCG 
ATGTACTGCG TGAAAGAGCT GCAGTGTGAG GGATCAGAGC TGTCATTTGA AGAGCTGCGA 
GCCCGACGAT ACTTCGATAA ATGCAGACAG CAAGAAGAAC AGCGCCGGCT GGCGGAGGAG 
CAGCAGCGGC TCTGGGAAGA GGAAGAGGAG GTGAGGAAGC TTAAGCAGCT GCTGGATGAT 
CTGGAGAGGA ACATGAGTCT AAAGCCAGCT GAACAGACAG ACCCTGTGAC GCCCGCTGTT 
ATGGAGCAGC AGGGCTTAGC CTTTCAGCAG GCTGATCCCC CTGTCACGAG CTATTCCCCT 
GCGGAGCCGT TTCTCACTGC AGCACTTGAC TCCAGACCTG AGCGAGAGAG TGTCCTGGAG 
CAGCCGGACA GGCCTTCAGA GATCCAGAGC AGCGTTCCTC AAAGCAGACA AGAGGATGTG 
TGTGTCCAGG GGGAAGCAGA CACATCGTAT GGTGGGTTCA ATCACTCTCA TGTCACCCCC 
AACACATCTC TGGGGTTCGT TCAGGCCACA CCGTCCCGGG TTCTGCCTTC ACCCACCGTC 
AACACACGCG AGGCTTTGGG TGTGATCATG GACATGTTTC AAGCGCCGAC GCTCCTCCAG 
GAGTCCATGT TCAACAGCAG CGTTCAGCCT GAAGACAGCT TCGAGAAGAG CTGCCGGATC 
AGTAGCGCCC CCTCCTGTGT AAAAGCTGCC AGCGCAACTC CGTTCATGAT CTACCAGGAC 
CGAGATCAGT CACAGAGCGC AGGGTCTGAT CAGGCTGGAA ACAAGCCAGC GGTGACCCGA 
GCACTGATGG AAATCCCAGT GTCCAAAGCC AATGAGACTC CAGTCAGCAC AGAGTTCATC 
ACGGATGAGA GCACCATGTG GGGTGCACGA AACGTCTCTG TGGGCCCATG TCCGAACCAT 
ACACGGGATT TCAGCCTGTC TGCTCACCTG GTGTCCACAC CGCTGCACCC TCATGCTCCA 
CACTCGTGGG ATGCAGAGCA GAGCCAAGAG GAAAACGCAG TGGTCAGAGG CGTCAGCGCT 
GCAGAGGAAA CCCCATACCT CAGACAGCCC ACCAAACTCA GCCCAATCAT GGAGCAGAGC 
CCAGCGGAGG AGAAGCCGGC CGAGTGCAGC ATGCAGGCTC AGGGCACTAT AGTTGGGGAG 
GGGGTGTCTT TAGCACAGCA GAGTCAAGGA CTTTCTCTGG CCCAGAGCAG CTGCAGCAGC 
AGACACCCGC TGGCTGCTTT GTCCTTTCCC GACCACACGG CCCTCCGAGC ACAGGACGAG 
TCCTTGAGGA ACAGCAGCAG ACCCGCCTGG AGCATCTATC AGAGCCCAGA GAAGGAGCCC 
TCGCTTCTGG ACACAGCCAA AGATGAAGAC CTACTGCCCA CGACCTCCTT CCACAGGCAT 
TCGTCAGGGA TGAAGCCTTT GGAGACACCG GATATTCCAG ACATGAGCCC CAGAGCCGCT 
CCTGGACTCA ACTGGTTCCA GATGGACAGC TCCACACATG CAGCAGAACC AGATTTGGAT 
GTCATGATGA GTCCCCCTCC TGTCCCTGGA GCCTGGAACA TCTACCAGAG TCCGGAGAAA 
CCACCCAAAA CAGACTTCCT GTGGGCCATG AGCCCTAAGC CAATAGCAGA GCATGCTTTA 
CACATGGAGG AAGTGGACGT TCTGCTTCCT GAAAGGTCTT TCCTGCAGCG GGAGCCAGGC 
GTGGAAGCAC TGCAAGTGTT GCATGAGTCT CTCCTGATGA GTCCCAAGCG AACCCCAGCA 
GCCGCTCAGG AGGAGCACTG GAGTCCAAGA GCAGACTGGT CACACACTGC CAGCCCACAG 
CGGCCAGTAG AACAGCATCT GAATGTCATG ACTAGCCCCC CGCAGGGCGC CAGGACCCCC 
AGAGCACCCA TGAGCCCCAT GGACACATCC AGAGCAGTGT GTGTTCCTTT GGTGCCGGAC 
CCTTGGGACG AGGAGCTGAT TGTGTCTCTG CTCTCGAACC TGCAGCCTCC TCTCTCCTCA 
CAGCCCAACC TCACCGTCTG GAGCTGCCAC CTGCCCTCCA TCACTCCCAA AATGACCGTC 
CAGATCGCTG GAGAGTCTCT GCGTGTGGAC TTTGTTCTGG GACAGGGAGC GTTCGCCACC 
GTCTACCAGG CCACCAACCT CATGACCACA CAGAAGCTCT TCCTCAAGGT GCAGAAGCCT 
GCGAACCCCT GGGAGTTTTA CATTGACTGT CAGCTGAACA AGCGTCTGCA GCCCAGCGAG 
AGGCATCTCT ACAACAGCAT CCACTCCGCT CATCTGTTCA ACAACGGCAG CGTCCTCGTC 
GGACAACTGC ACAACTGCGG CACACTGCTG AACGCTATAA ACCTGTACAA GAGGCGCAGT 
GAGAAGCTGA TGCCGCAGCC GCTGGTGCTG TACTTCAGCG TCTGCATCCT GAAGATGGTG 
GAGCTGCTGC ATGCGGCACA CATCATCCAC GCAGACATCA AGCCCGACAA CTTCCTGCTG 
GGTGAGAGGT TTCTGGAGAA CGACTGCTTT GACCAGGAAA ACCTGCAGCA TGGCCTGGCT 
CTCATCGACC TGGGCCAGAG CATCGACATG ACACTCTTCC CGGAAGGCAC TGCGTTCACG 
GCCAAATGCA TGACCTCCGG CTTCCAGTGC GTGGAGATGC TGAGCGGCCG ACCCTGGACC 
TACCAGACCG ATTATTTCGG GATCGCAGGG ACGGTTTACT GTATGTTGTT TGGGACGTAC 
ATGGAAGTCA AACAGGAAGG CGATGTTTGG AGGACAAATG GAGTCTTTAA AAGGAACGTT 
CACGCTGATC TCTGGCAGGA TTTCTTCCAC ACGCTGCTGA ACGTGTCGGA CTGCAGCGCT 
CCTCCGTGTC TGAGCGTCCT CCGCCAGCGG CTGCACGCCG TCCTGCAGCA GAACTACAAC 
AGCAAGCTGC GCAGCCTGAA GAACCGGCTG GTGGTGCAGA TCCTGGAGGA GGCCCGATCC 
ACACGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_017352663.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208152.1 Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa56697
Clone ID Related Accession (Same CDS sequence)	XM_017352663.1
Accession Version	XM_017352663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATGCA TAATGGTCTT AATAAGGTCT TTAACTGTGT TTAAATTGAT GATGATGTAT 
GTGTGTGACT TCAGTAAGCC TTTTAAAAGC TTATACTTGT GTGTCTTCAG TCATGATGTG 
TGTGTGTTTG TTCATCGTCA CTGCAGGGCC TTTGAGCTGA GCGTACGCAG TTATGCTGGA 
GATGATCCGC TCGACCCCTG GGACAAGTTT GTACACTTTC TGGAGAGCAG GATGTCAGCG 
GAGGAGAAGA AAGGTCTTTC TGTGGTGCTG GAGAGACTAG TTCAGTCCTT TCTTCAAGAC 
GAGCGCTACC ACAATGACGT GCGATACGTC ACTCACTGCA TCACCTGTGC AAACTTATAC 
AGCGATCCCA TGGAAGTGTA CAGTTATTTG CACAGCCGCG GTGTGGGGAC GCAGACGGCC 
GCACTCTACA TTGACTGGGC TCAGCAGTGC GAGAAGAAAG GGCAGATGGT GCAGGCTGAG 
ATGGTGTATA ACAGGGCGCT GATGAACAAG GCTCAGCCGC AGGACACTGT GCAGGAGCAG 
TACAGGTTCT TCCAGGAAAG GACGTCTGCG AGCACTCCGG CAGCACCGTC AGATCACGTC 
CGCCATCCTC TTCAAAACTC CAGGATGGTA AACCAGAACA CCCGACCCAG AGACAATGTT 
CAGCCGCAGT GCAAGGAACC AGAAGCGGCG TGTCCTGCTC CGAGAATGGT CTCCGTCATC 
TCTCGGTCTG AGAATAACCC GGTGCGGCCA GCCGGCTATA CCGCCCCTGA GACGGTGTCG 
ATGTACTGCG TGAAAGAGCT GCAGTGTGAG GGATCAGAGC TGTCATTTGA AGAGCTGCGA 
GCCCGACGAT ACTTCGATAA ATGCAGACAG CAAGAAGAAC AGCGCCGGCT GGCGGAGGAG 
CAGCAGCGGC TCTGGGAAGA GGAAGAGGAG GTGAGGAAGC TTAAGCAGCT GCTGGATGAT 
CTGGAGAGGA ACATGAGTCT AAAGCCAGCT GAACAGACAG ACCCTGTGAC GCCCGCTGTT 
ATGGAGCAGC AGGGCTTAGC CTTTCAGCAG GCTGATCCCC CTGTCACGAG CTATTCCCCT 
GCGGAGCCGT TTCTCACTGC AGCACTTGAC TCCAGACCTG AGCGAGAGAG TGTCCTGGAG 
CAGCCGGACA GGCCTTCAGA GATCCAGAGC AGCGTTCCTC AAAGCAGACA AGAGGATGTG 
TGTGTCCAGG GGGAAGCAGA CAGTGTGATC ATGGACATGT TTCAAGCGCC GACGCTCCTC 
CAGGAGTCCA TGTTCAACAG CAGCGTTCAG CCTGAAGACA GCTTCGAGAA GAGCTGCCGG 
ATCAGTAGCG CCCCCTCCTG TGTAAAAGCT GCCAGCGCAA CTCCGTTCAT GATCTACCAG 
GACCGAGATC AGTCACAGAG CGCAGGGTCT GATCAGGCTG GAAACAAGCC AGCGGTGACC 
CGAGCACTGA TGGAAATCCC AGTGTCCAAA GCCAATGAGA CTCCAGTCAG CACAGAGTTC 
ATCACGGATG AGAGCACCAT GTGGGGTGCA CGAAACGTCT CTGTGGGCCC ATGTCCGAAC 
CATACACGGG ATTTCAGCCT GTCTGCTCAC CTGGTGTCCA CACCGCTGCA CCCTCATGCT 
CCACACTCGT GGGATGCAGA GCAGAGCCAA GAGGAAAACG CAGTGGTCAG AGGCGTCAGC 
GCTGCAGAGG AAACCCCATA CCTCAGACAG CCCACCAAAC TCAGCCCAAT CATGGAGCAG 
AGCCCAGCGG AGGAGAAGCC GGCCGAGTGC AGCATGCAGG CTCAGGGCAC TATAGTTGGG 
GAGGGGGTGT CTTTAGCACA GCAGAGTCAA GGACTTTCTC TGGCCCAGAG CAGCTGCAGC 
AGCAGACACC CGCTGGCTGC TTTGTCCTTT CCCGACCACA CGGCCCTCCG AGCACAGGAC 
GAGTCCTTGA GGAACAGCAG CAGACCCGCC TGGAGCATCT ATCAGAGCCC AGAGAAGGAG 
CCCTCGCTTC TGGACACAGC CAAAGATGAA GACCTACTGC CCACGACCTC CTTCCACAGG 
CATTCGTCAG GGATGAAGCC TTTGGAGACA CCGGATATTC CAGACATGAG CCCCAGAGCC 
GCTCCTGGAC TCAACTGGTT CCAGATGGAC AGCTCCACAC ATGCAGCAGA ACCAGATTTG 
GATGTCATGA TGAGTCCCCC TCCTGTCCCT GGAGCCTGGA ACATCTACCA GAGTCCGGAG 
AAACCACCCA AAACAGACTT CCTGTGGGCC ATGAGCCCTA AGCCAATAGC AGAGCATGCT 
TTACACATGG AGGAAGTGGA CGTTCTGCTT CCTGAAAGGT CTTTCCTGCA GCGGGAGCCA 
GGCGTGGAAG CACTGCAAGT GTTGCATGAG TCTCTCCTGA TGAGTCCCAA GCGAACCCCA 
GCAGCCGCTC AGGAGGAGCA CTGGAGTCCA AGAGCAGACT GGTCACACAC TGCCAGCCCA 
CAGCGGCCAG TAGAACAGCA TCTGAATGTC ATGACTAGCC CCCCGCAGGG CGCCAGGACC 
CCCAGAGCAC CCATGAGCCC CATGGACACA TCCAGAGCAG TGTGTGTTCC TTTGGTGCCG 
GACCCTTGGG ACGAGGAGCT GATTGTGTCT CTGCTCTCGA ACCTGCAGCC TCCTCTCTCC 
TCACAGCCCA ACCTCACCGT CTGGAGCTGC CACCTGCCCT CCATCACTCC CAAAATGACC 
GTCCAGATCG CTGGAGAGTC TCTGCGTGTG GACTTTGTTC TGGGACAGGG AGCGTTCGCC 
ACCGTCTACC AGGCCACCAA CCTCATGACC ACACAGAAGC TCTTCCTCAA GGTGCAGAAG 
CCTGCGAACC CCTGGGAGTT TTACATTGAC TGTCAGCTGA ACAAGCGTCT GCAGCCCAGC 
GAGAGGCATC TCTACAACAG CATCCACTCC GCTCATCTGT TCAACAACGG CAGCGTCCTC 
GTCGGACAAC TGCACAACTG CGGCACACTG CTGAACGCTA TAAACCTGTA CAAGAGGCGC 
AGTGAGAAGC TGATGCCGCA GCCGCTGGTG CTGTACTTCA GCGTCTGCAT CCTGAAGATG 
GTGGAGCTGC TGCATGCGGC ACACATCATC CACGCAGACA TCAAGCCCGA CAACTTCCTG 
CTGGGTGAGA GGTTTCTGGA GAACGACTGC TTTGACCAGG AAAACCTGCA GCATGGCCTG 
GCTCTCATCG ACCTGGGCCA GAGCATCGAC ATGACACTCT TCCCGGAAGG CACTGCGTTC 
ACGGCCAAAT GCATGACCTC CGGCTTCCAG TGCGTGGAGA TGCTGAGCGG CCGACCCTGG 
ACCTACCAGA CCGATTATTT CGGGATCGCA GGGACGGTTT ACTGTATGTT GTTTGGGACG 
TACATGGAAG TCAAACAGGA AGGCGATGTT TGGAGGACAA ATGGAGTCTT TAAAAGGAAC 
GTTCACGCTG ATCTCTGGCA GGATTTCTTC CACACGCTGC TGAACGTGTC GGACTGCAGC 
GCTCCTCCGT GTCTGAGCGT CCTCCGCCAG CGGCTGCACG CCGTCCTGCA GCAGAACTAC 
AACAGCAAGC TGCGCAGCCT GAAGAACCGG CTGGTGGTGC AGATCCTGGA GGAGGCCCGA 
TCCACACGCA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208152.1
CDS	199..3813
Translation	MSCIMVLIRSLTVFKLMMMYVCDFSKPFKSLYLCVFSHDVCVFVHRHCRAFELSVRSYAGDDPLDPWDKFVHFLESRMSAEEKKGLSVVLERLVQSFLQDERYHNDVRYVTHCITCANLYSDPMEVYSYLHSRGVGTQTAALYIDWAQQCEKKGQMVQAEMVYNRALMNKAQPQDTVQEQYRFFQERTSASTPAAPSDHVRHPLQNSRMVNQNTRPRDNVQPQCKEPEAACPAPRMVSVISRSENNPVRPAGYTAPETVSMYCVKELQCEGSELSFEELRARRYFDKCRQQEEQRRLAEEQQRLWEEEEEVRKLKQLLDDLERNMSLKPAEQTDPVTPAVMEQQGLAFQQADPPVTSYSPAEPFLTAALDSRPERESVLEQPDRPSEIQSSVPQSRQEDVCVQGEADSVIMDMFQAPTLLQESMFNSSVQPEDSFEKSCRISSAPSCVKAASATPFMIYQDRDQSQSAGSDQAGNKPAVTRALMEIPVSKANETPVSTEFITDESTMWGARNVSVGPCPNHTRDFSLSAHLVSTPLHPHAPHSWDAEQSQEENAVVRGVSAAEETPYLRQPTKLSPIMEQSPAEEKPAECSMQAQGTIVGEGVSLAQQSQGLSLAQSSCSSRHPLAALSFPDHTALRAQDESLRNSSRPAWSIYQSPEKEPSLLDTAKDEDLLPTTSFHRHSSGMKPLETPDIPDMSPRAAPGLNWFQMDSSTHAAEPDLDVMMSPPPVPGAWNIYQSPEKPPKTDFLWAMSPKPIAEHALHMEEVDVLLPERSFLQREPGVEALQVLHESLLMSPKRTPAAAQEEHWSPRADWSHTASPQRPVEQHLNVMTSPPQGARTPRAPMSPMDTSRAVCVPLVPDPWDEELIVSLLSNLQPPLSSQPNLTVWSCHLPSITPKMTVQIAGESLRVDFVLGQGAFATVYQATNLMTTQKLFLKVQKPANPWEFYIDCQLNKRLQPSERHLYNSIHSAHLFNNGSVLVGQLHNCGTLLNAINLYKRRSEKLMPQPLVLYFSVCILKMVELLHAAHIIHADIKPDNFLLGERFLENDCFDQENLQHGLALIDLGQSIDMTLFPEGTAFTAKCMTSGFQCVEMLSGRPWTYQTDYFGIAGTVYCMLFGTYMEVKQEGDVWRTNGVFKRNVHADLWQDFFHTLLNVSDCSAPPCLSVLRQRLHAVLQQNYNSKLRSLKNRLVVQILEEARSTRR

Target ORF information:

RefSeq Version	XM_017352663.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352663.1

ORF Insert Sequence:

ATGTCATGCA TAATGGTCTT AATAAGGTCT TTAACTGTGT TTAAATTGAT GATGATGTAT 
GTGTGTGACT TCAGTAAGCC TTTTAAAAGC TTATACTTGT GTGTCTTCAG TCATGATGTG 
TGTGTGTTTG TTCATCGTCA CTGCAGGGCC TTTGAGCTGA GCGTACGCAG TTATGCTGGA 
GATGATCCGC TCGACCCCTG GGACAAGTTT GTACACTTTC TGGAGAGCAG GATGTCAGCG 
GAGGAGAAGA AAGGTCTTTC TGTGGTGCTG GAGAGACTAG TTCAGTCCTT TCTTCAAGAC 
GAGCGCTACC ACAATGACGT GCGATACGTC ACTCACTGCA TCACCTGTGC AAACTTATAC 
AGCGATCCCA TGGAAGTGTA CAGTTATTTG CACAGCCGCG GTGTGGGGAC GCAGACGGCC 
GCACTCTACA TTGACTGGGC TCAGCAGTGC GAGAAGAAAG GGCAGATGGT GCAGGCTGAG 
ATGGTGTATA ACAGGGCGCT GATGAACAAG GCTCAGCCGC AGGACACTGT GCAGGAGCAG 
TACAGGTTCT TCCAGGAAAG GACGTCTGCG AGCACTCCGG CAGCACCGTC AGATCACGTC 
CGCCATCCTC TTCAAAACTC CAGGATGGTA AACCAGAACA CCCGACCCAG AGACAATGTT 
CAGCCGCAGT GCAAGGAACC AGAAGCGGCG TGTCCTGCTC CGAGAATGGT CTCCGTCATC 
TCTCGGTCTG AGAATAACCC GGTGCGGCCA GCCGGCTATA CCGCCCCTGA GACGGTGTCG 
ATGTACTGCG TGAAAGAGCT GCAGTGTGAG GGATCAGAGC TGTCATTTGA AGAGCTGCGA 
GCCCGACGAT ACTTCGATAA ATGCAGACAG CAAGAAGAAC AGCGCCGGCT GGCGGAGGAG 
CAGCAGCGGC TCTGGGAAGA GGAAGAGGAG GTGAGGAAGC TTAAGCAGCT GCTGGATGAT 
CTGGAGAGGA ACATGAGTCT AAAGCCAGCT GAACAGACAG ACCCTGTGAC GCCCGCTGTT 
ATGGAGCAGC AGGGCTTAGC CTTTCAGCAG GCTGATCCCC CTGTCACGAG CTATTCCCCT 
GCGGAGCCGT TTCTCACTGC AGCACTTGAC TCCAGACCTG AGCGAGAGAG TGTCCTGGAG 
CAGCCGGACA GGCCTTCAGA GATCCAGAGC AGCGTTCCTC AAAGCAGACA AGAGGATGTG 
TGTGTCCAGG GGGAAGCAGA CAGTGTGATC ATGGACATGT TTCAAGCGCC GACGCTCCTC 
CAGGAGTCCA TGTTCAACAG CAGCGTTCAG CCTGAAGACA GCTTCGAGAA GAGCTGCCGG 
ATCAGTAGCG CCCCCTCCTG TGTAAAAGCT GCCAGCGCAA CTCCGTTCAT GATCTACCAG 
GACCGAGATC AGTCACAGAG CGCAGGGTCT GATCAGGCTG GAAACAAGCC AGCGGTGACC 
CGAGCACTGA TGGAAATCCC AGTGTCCAAA GCCAATGAGA CTCCAGTCAG CACAGAGTTC 
ATCACGGATG AGAGCACCAT GTGGGGTGCA CGAAACGTCT CTGTGGGCCC ATGTCCGAAC 
CATACACGGG ATTTCAGCCT GTCTGCTCAC CTGGTGTCCA CACCGCTGCA CCCTCATGCT 
CCACACTCGT GGGATGCAGA GCAGAGCCAA GAGGAAAACG CAGTGGTCAG AGGCGTCAGC 
GCTGCAGAGG AAACCCCATA CCTCAGACAG CCCACCAAAC TCAGCCCAAT CATGGAGCAG 
AGCCCAGCGG AGGAGAAGCC GGCCGAGTGC AGCATGCAGG CTCAGGGCAC TATAGTTGGG 
GAGGGGGTGT CTTTAGCACA GCAGAGTCAA GGACTTTCTC TGGCCCAGAG CAGCTGCAGC 
AGCAGACACC CGCTGGCTGC TTTGTCCTTT CCCGACCACA CGGCCCTCCG AGCACAGGAC 
GAGTCCTTGA GGAACAGCAG CAGACCCGCC TGGAGCATCT ATCAGAGCCC AGAGAAGGAG 
CCCTCGCTTC TGGACACAGC CAAAGATGAA GACCTACTGC CCACGACCTC CTTCCACAGG 
CATTCGTCAG GGATGAAGCC TTTGGAGACA CCGGATATTC CAGACATGAG CCCCAGAGCC 
GCTCCTGGAC TCAACTGGTT CCAGATGGAC AGCTCCACAC ATGCAGCAGA ACCAGATTTG 
GATGTCATGA TGAGTCCCCC TCCTGTCCCT GGAGCCTGGA ACATCTACCA GAGTCCGGAG 
AAACCACCCA AAACAGACTT CCTGTGGGCC ATGAGCCCTA AGCCAATAGC AGAGCATGCT 
TTACACATGG AGGAAGTGGA CGTTCTGCTT CCTGAAAGGT CTTTCCTGCA GCGGGAGCCA 
GGCGTGGAAG CACTGCAAGT GTTGCATGAG TCTCTCCTGA TGAGTCCCAA GCGAACCCCA 
GCAGCCGCTC AGGAGGAGCA CTGGAGTCCA AGAGCAGACT GGTCACACAC TGCCAGCCCA 
CAGCGGCCAG TAGAACAGCA TCTGAATGTC ATGACTAGCC CCCCGCAGGG CGCCAGGACC 
CCCAGAGCAC CCATGAGCCC CATGGACACA TCCAGAGCAG TGTGTGTTCC TTTGGTGCCG 
GACCCTTGGG ACGAGGAGCT GATTGTGTCT CTGCTCTCGA ACCTGCAGCC TCCTCTCTCC 
TCACAGCCCA ACCTCACCGT CTGGAGCTGC CACCTGCCCT CCATCACTCC CAAAATGACC 
GTCCAGATCG CTGGAGAGTC TCTGCGTGTG GACTTTGTTC TGGGACAGGG AGCGTTCGCC 
ACCGTCTACC AGGCCACCAA CCTCATGACC ACACAGAAGC TCTTCCTCAA GGTGCAGAAG 
CCTGCGAACC CCTGGGAGTT TTACATTGAC TGTCAGCTGA ACAAGCGTCT GCAGCCCAGC 
GAGAGGCATC TCTACAACAG CATCCACTCC GCTCATCTGT TCAACAACGG CAGCGTCCTC 
GTCGGACAAC TGCACAACTG CGGCACACTG CTGAACGCTA TAAACCTGTA CAAGAGGCGC 
AGTGAGAAGC TGATGCCGCA GCCGCTGGTG CTGTACTTCA GCGTCTGCAT CCTGAAGATG 
GTGGAGCTGC TGCATGCGGC ACACATCATC CACGCAGACA TCAAGCCCGA CAACTTCCTG 
CTGGGTGAGA GGTTTCTGGA GAACGACTGC TTTGACCAGG AAAACCTGCA GCATGGCCTG 
GCTCTCATCG ACCTGGGCCA GAGCATCGAC ATGACACTCT TCCCGGAAGG CACTGCGTTC 
ACGGCCAAAT GCATGACCTC CGGCTTCCAG TGCGTGGAGA TGCTGAGCGG CCGACCCTGG 
ACCTACCAGA CCGATTATTT CGGGATCGCA GGGACGGTTT ACTGTATGTT GTTTGGGACG 
TACATGGAAG TCAAACAGGA AGGCGATGTT TGGAGGACAA ATGGAGTCTT TAAAAGGAAC 
GTTCACGCTG ATCTCTGGCA GGATTTCTTC CACACGCTGC TGAACGTGTC GGACTGCAGC 
GCTCCTCCGT GTCTGAGCGT CCTCCGCCAG CGGCTGCACG CCGTCCTGCA GCAGAACTAC 
AACAGCAAGC TGCGCAGCCT GAAGAACCGG CTGGTGGTGC AGATCCTGGA GGAGGCCCGA 
TCCACACGCA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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bub1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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