lyst cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lyst
Entrez Gene ID	100150726
Full Name	lysosomal trafficking regulator
Synonyms	si:ch211-30i2.1
General protein information	Preferred Names lysosomal trafficking regulator Names lysosomal-trafficking regulator
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358861.1	XP_017214350.1	lysosomal-trafficking regulator isoform X1
XM_017358861.2	XP_017214350.1	lysosomal-trafficking regulator isoform X1
XM_009307601.2	XP_009305876.1	lysosomal-trafficking regulator isoform X2
XM_021472744.1	XP_021328419.1	lysosomal-trafficking regulator isoform X2
XM_017358862.1	XP_017214351.1	lysosomal-trafficking regulator isoform X3
XM_009307601.3	XP_009305876.1	lysosomal-trafficking regulator isoform X2
XM_017358862.2	XP_017214351.1	lysosomal-trafficking regulator isoform X3

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC718051	XP_002808311.1
Canis lupus familiaris (dog)	LYST	XP_005618856.1
Rattus norvegicus (Norway rat)	Lyst	NP_445970.1
Danio rerio (zebrafish)	lyst	XP_001919653.2
Bos taurus (cattle)	LYST	NP_776445.1
Gallus gallus (chicken)	LYST	XP_419558.4
Homo sapiens (human)	LYST	NP_000072.2
Mus musculus (house mouse)	Lyst	NP_034878.2
Xenopus tropicalis (tropical clawed frog)	lyst	XP_002935877.2
Pan troglodytes (chimpanzee)	LYST	XP_514288.2

Gene Ontology

The following lyst gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lyst cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145579	XM_021472744.1 Latest version!	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa48897	XM_009307601.3 Latest version!	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa54787	XM_017358861.2 Latest version!	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa54788	XM_017358862.2 Latest version!	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa54787	XM_017358861.1	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa48897	XM_009307601.2	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa54788	XM_017358862.1	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lyst ( XM_021472744.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328419.1 Danio rerio lysosomal trafficking regulator (lyst), transcript variant X2, mRNA.

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CloneID	ODa145579
Clone ID Related Accession (Same CDS sequence)	XM_021472744.1
Accession Version	XM_021472744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lysosomal-trafficking regulator isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394890.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTTAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACGC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA GCTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTCGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTC CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAAACAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTATTGAG CCATCACAAC CTCACCTGAG ACTTTTTAGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGAAGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGTCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTA 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTGGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAACGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAAAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGCACCG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCAGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CCGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGACCA GTCCAGTGTT CCAGAGGCAG TTCTTCAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCTTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTG 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCCGAAGT CATTGAGGAG AAACCTGACA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACT TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CGGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGATTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
TCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CTGGCTCCAT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGTGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTA AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCTTTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
CTCGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAT 
CTGCTGCGGC TGGTTTATAA TTTCCTGTTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTGTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTACT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC ATGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
CGTAGACGCT TCACACTGGC CCAGTCGGAC TCTCTGCTGA CCCGCATGCG TTCTGTAGCC 
AGTGATGAGC TGACACAGAT GATGCAGCGC AGGATGAGTC AAGAAAACCC CATCAGAGCC 
AGCGAGACCG AGCTCATCCA GAGAATGCAG AGACTGGTGG TTCTGGCTGT CAACAGACTC 
ATTTATCAAG ACATCAGCCA TGATTTCTTT GACCTGCTGA ACTTCCCTGA GTCTCCTGAT 
CACTCGATGT CCATGCCAAC AGCAGGAGAT CAGACATCTG ATGAGAACGG CTCCACTCCG 
TCTACCCCGA TCCCAGCCGG AAACATCAAG AGCTTCCGGA AGGACATTCT CAGACTGATG 
ATGGAGGGCA TAAAGATCGG CCTGGGCAAC ACTGGTCGTG GCGGCGCTCC TCGTCAGCAG 
TGGCGGCGAA TCCTCTGGTC CTGCCGAGAC ACGTTCAGAG TGCAGATCGG CCGCATGCTG 
GTGCACACCC TCAGTCCTGA ACTTCCTCTG GAGGACAGGA AGGAGGCGCT AGAGTTCGTC 
CATGAGCAGA ACTACTCAGA GATCCTCAGA GAAAGCCTCA GCCCTGGCCT CGAGCATGGG 
CCCAAGCTGG CTCTGTATCT GTACGAGCTG CTGCACGATC ACAAAGACGC CCTGAGTAGA 
GAAGAGCAGG CGGCAGGAAA CACCTTCATG ACGGCTCTGA AACTCTGCGG ACACCGCTGC 
ATCCCACCCA GCGCTCCAGC CAAACCTGAC CTCCTGAAAG CCATCAAAGA GGAGCAACTG 
AAGTATGAAT CAGAGGCAAG AACCAGCAAA GGAGCCTGGG AAAAGAAGCT GTCCAACACA 
CAGAGGAGCC TGTTGCAGAG GCTGGACGGC AAGTCGAAGG ACATCTCAAA GATTGCGGCG 
GACATCACTC AGAACGTGTC TCTCAGGCAG GGCATGGAGA GGAAGAAGGT CATGCAGCAC 
ATCAGAGGCC TGTATAAGAC TGACCTGAGC GCTAGTAGAC ACTGGCAGGA GCTCGTCCAG 
CAGCTCACAC ACGACCGGGC GGTGTGGTAC GATCAGTCCT CTTATCCCAC TTCCTGGCAG 
CTGGACCCCA CCGAGGGCCC GAACCGAGAG CGGAGACGCC TCCAGCGCTG CTACCTGACC 
ATCCCCAACA AATATCTGCT CAAAGACCGT CGCAAACTAG ACGATTCCAT TAAGGCTCCT 
CTGGCCTTCC TGTTTGAGGA CAAGACTCAC TCCTCTTCAT CTATAGTGAA GGATAAAGCC 
ACCAGTGAGC CCATCAGGTT CACAAGAAGG TGCGTAAGTG TGGCTCCCTC CAGAGAGACA 
GCAGGGGAGC TCTTACTGGG AAAATCTGGG ATGTATTTTG TGGAGGAAAA TGTTCCTGAG 
ACTCACGATA ACCAGAGTCC TCATGGAGAG ACGGAGCCGG CCTCTTTCTC CTGGACGTAT 
GAGGAGATAA AGGAGGTTCA CAAGCGCTGG TGGCAGCTCA GAGACAACGC CATGGAGATC 
TTCCTGACCA ATGGAAGGAC ACTGCTGCTG GCCTTTGACA ACACAAAGTT TCGTGATGAC 
GTCTACCACA ACATCCTGAC CTCAGATCTG CCCAACCTGC TGGAGTATGG AAACATCAGC 
GCCCTCACGC ACCTCTGGAG CTCAGGACAG ATCACCAACT TTGAGTACCT GACGCATCTC 
AACAAGCACG CGGGACGCTC CTTCAATGAC CTCATGCAGT ATCCCGTCTT CCCCTTCATC 
CTCAAAGATT ACACCAATGA GACGCTCGAC CTGCAGGAGG CCTCTATTTA CAGGAACCTC 
AGCAAGCCCA TTGCAGTGCA GTCTAAAGAG AAGGAAGACC GTTATGTGGA CAATTACAGG 
TATCTGGAGG AGGAGTATAA GAAAGGCAAG AGAGAGGACG ACCCTATGCC CCCTGTCCAG 
CCTTATCACT ACGGCTCTCA TTACTCCAAC AGCGGCACCG TCCTGCACTT CCTGGTCCGA 
CTGCCTCCTT TTACCAAAAT GTTTCTGGCA TATCAAGACC AGAGCTTTGA TATTCCTGAC 
CGAACCTTCC ACTCCATGAA CACCACGTGG CGTCTGTCGT CCTACGAGTC CATGACTGAT 
GTCAAAGAGC TCATCCCAGA GTTCTTCTAC CTGCCCGAGT TCCTGGTCAA CAGAGAAGGT 
TTTGACTTTG GCGTGCGGCA GAACAGCGAG CGGGTCAACC ATGTGAATCT GCCGCCGTGG 
GCCAGAAATG ACCCGCGACT CTTCATCCTC ATCCACAGAC AGGCTCTGGA GTCCGATCAA 
GTCTCGCAGA CGCTCTGCCA GTGGATCGAC CTGGTGTTTG GACTCAAGCA GAAAGGCAAA 
GCTGCGGTCC AGGCCATCAA CGTCTTTCAT CCTGCAACGT ACTTTGGTAT GGACGTGTCT 
GCAGTTGAAG ACCCTGTGCA GCGGCGGGCT CTAGAAACCA TGATCAAGAC GTACGGACAA 
ACACCCAGGC AGCTGTTCAG CGCTTCTCAC ATCAGCAGAG CCGGAACCAA ACTGCTGTCA 
GAGGCTGAGC TGCCCGCCGC CATGGGTCTG CTGGTGCAGC TGGCCTTCAG AGAGAGCCGA 
GAACAGCCCC GGGACAGCGG CTACCCGAGT CCTCTGCCGT GGATAAAGGG CTTGAAATGG 
GGCGAGTATG TGGGTTCACC CAGTGCTCCG GACCCTGTGG TCTGTTTCAG TCAACCTCAC 
GGCGAACGCT TCGGCTCCCT GCTGGCACTG CCCACACGTG CCATCTGCGG CCTCTCACGC 
AAGTTCTGCC TCATGATGAT CTACAGCAAA GAGCAAGGTG TGAGGAGCAT GCACAGCACT 
GACATACAGT GGTCTGCTAT TCTGAGCTGG GGTTACACCG ACAACATGCT GCGTCTGAAG 
AGCAAACAGA GCGAACCACC AATCAACTTC ATCCAGTGCT CACCGCTGCA CCAGGTGAGC 
AGTTGTGCCT GGGTGCCGGA CAGTTGTCAG CTGTTTACAG GCAGCAAGTG TGGAGTCATT 
ACCGCCTACA GCAACCGCTT CACCACCACC ATGCCCACAG AGATGGAGGT GGAGTCGCAG 
GTTGATCTGT ACGGACACAC AGCCGAGGTC ACGGGTCTGT TCGTCTGTAA ACCCTACAGT 
ATCCTCATCA GTGTGAGTCG GGACGGAACC TGCATACTCT GGGATCTCAA CAGGTTGTGT 
TACGTACAGA GTCTCAGCGG TCACAAGAGT CCAGTGAACG CGGTGTCAGC CAGCGAGACC 
ACCGGAGACA TCGCCACCGT CTGCGACTCA GTGGGTGGAG GAAGTGACCT GCGGCTGTGG 
ACCATTAATG GAGATCTGAT TGGTCACGTT CACTGTCGTG AGATCATCTG TTCAGTGGCT 
TTCTCCAATC AGCCGGAGGG AGTTTCAGTC AATGTGATCG CAGGCGGACT GGAGAACGGG 
GTGGTCAGGT TGTGGAGCAC GTGGGACCTG AAGCCAGTCA GAGAGATCAC GTTCCCAAAG 
TCCAACAGAC CTATCATAAG TCTGACGTTC TCATGTGACG GTCATCATCT ATACACGGCT 
AACAGTGAGG GCACGGTGAT CGCGTGGTGT CGGCGAGACC AGCAGCGCAT GAAGCTGCCC 
ATGTTTTACT CTTTCCTCAG CAGTTACGCC GCAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328419.1
CDS	202..11700
Translation	MASSHLADLSQAWENYMDCRTQGADLQVSLQCLESFLCTFHCVRHLSDPRAKDALKFCSDMSGASNTLAREFLTDVHQLCSAVAQRAEDEEESHMVALGEYLVRGRGFLLLSTLESVIDQELTCREELLTLLLSLLPLVWKIPVQEEKAPDFTLPSLLEVFLCQEGRTISQKAGQDPCDVQNSPKRLSGSWKSRRSRRTAQRYSVRDARKSQLSTSDSEANSDDKTTNTGSKHRRFHSTAIHVSCQLHQSNNSSQTAPQHPTLDAMGTIEPSQPHLRLFRSHTMDPNMLSDPTTLSIFNRMENSPFDLCHVLLSLLEKVCKFDMTINHNPGLAVSVVPTLTEILTDFGDCCGPSGGGGGGIEELAGGWTEEPIALVQRMLLRTILHLMFVDVGQNETLPDNLRRSLTDLLRATLKIRTCLERQADPFAPRPKKTLQEIQEDFSFSRYRHRALLLPELLQGVLQLLLGCLQASAPNPFFFSQSLELVHEFVQHRGLELFEVTALRLEALAEAHNSEISNEASEHLRCLIADVLKIISAVKRAKSEQLHQSVCARRRHRRCEYSHFLHHHRDLSGLPVSAFKQAARRNPFEEDSKGNDGEVRYPERCCCLAACAHQCLRLFQRLSPSGPAVLQVLAGVQAVGICCCMDPRSVIAPMLQAFQAPGLRSYQSHVLSVLSRLVLEQLGGGQPSEKAKLASCNICTLDSSQLPGLEETLQGCGPKKELNLSTSPSPSYRSQGILPSGGAEDILWKWDALEAYQELVFGEDWHLSQQIASHVCQLTLRGNAVVQWQLYTHIFNPVLQRGVELTHHAQQLGVSTTCSQVCSYHTHCLPVEVLLVYLQTLPALLKSSRVVRDLFLSCNGLNQVTELIYLDCTRPWALKVFETLILNAWRNHTESAVHELERAEAQEREAVLGLVEELSTRGTGDAPQSLTKFYEGLKEACPPPKGKSETSEFSGVGRNRGEAHLNTINLFLCVAFLCVSKEADSDRDSANDSEDTSGYDSTASEPLGGRLPCLSPDSVALPSKEQVRRAADVWSVCRWVYMTSPVFQRQFFRLGGLDVCSRLMTMVIQKLTSKNKDCKFKKKRESKGKSSPSREDSPTRCITGHFDNMVTNAGTAETQHPELSDTVSLSQTESKSSDPMHRLEEEWPFQSIRLLEALLAICLHSANSALQRLEPDLSFQLQSVEETLCEVRDQLSRSGVVNSELAVPLFDSLLRVALAEMFCSPEVIEEKPDKKCAVSEATPPAGDLSEDVDEAHVSAVQPLGEEEGYDADSESNPEDSTRQEEGLKVEAGVSLRECTLGMAGVPQGSVRGRLLFPEICLMELQLLASGSPDLEVLGHVLQSLLAAVKGHHNNAAQLYQQGGVKVILTGFPNILSQSDASYKECQTVLMELLVAMVSDRITAEELALLTRLFLEKTPPTEILLRGVLQIVEANTDTEPLYFMSFPMIPGSGGPGLSPSPGTRPHGATAGKGGVNTILRGKLPLGRGEADMSRPGHLRSSPWHAAPFHLPLVGQNCWPHMASGFSASLWVRAGKDEDGRSKKEDEVCVAEESPHGSAHVTEQQTERCLLHVLSMGSKALMLQVWVNISTRAFTFRICIDPNDEMKAGLLAQAESGDEVIPAGRWQHLALTYTQQPEGKKNIHGQLSLWVCGIRKCDVSLDFTLPRKSSLSSDSNKTYCMLGHYQHSAEEQASPSARWDMGTLMLFNGSRIDTTEAFYLYASGPDLTSIMPCKYGKPHGTLSKYVTQEGLQCEQIRELLMRNADVDISPLVESLAVVYTPSTPSLYTIYEPVIRLKGQAKSAVISQRPFSAKEVQSSILESSFLRTMMPCQAFGLHSALHKIGGTGSIVFLFARAVELCDCEKTQALALQVLLSLVKNNQHRTHEMECYHGYSMIHQVLIKPKCIVGYHILKTLLDGCCSGSILTIGEDGQFHLDAESTVVVQDLRLLSEILLDWKIWAKAQCGVWETLLTALEILIRVRHPQQMFNIRQLLKAQVVHRFLLACQVLQEHRDEYLTSIPQEVCLSFVKIIQEVLGSPPDMDLLRLVYNFLLAVHPPTNTYVCHTPTSFYFSLHIDGKLYQEKVQSMMYLRHSSSGGKSACSSVVSLSPTVFTEMPAEVRHTSSIEHEDDLSLRPRSTGPSPHSSPALTPRVTHGRVTGTTEPEGGASLSTQQALGSTETLKRAGDEQLLSSCESAKTICDSPIAGADEEGSIMGNRTPSISVEEEQDGPAGSPVESEEHFDWTSEEAPRRPDSLKGIQSFQRSHSNLASLGLAFPAQNGSLTIARWPTVADRAAPPDDWESYTYSPGYDRHSKADSSNDRSSAEDCLVLICCGLYDLLRGVLLLLPDLLLEEVMDKLIQPDALLVLVNHPSPLIQQGVMKLLDAYFSRAQKEQKEKFLKNHGFSLLANQLYQHQGSQGLLECFLEMLFGRPVGLEEDLDLEDMENISPFRKRCIIPVLGLLENSLYENSLVHNGLCLLLQLLNACPKLADILLDHGLLYVLFNTLSTLNGLENGIPPNDYRLVVCDIQQLLVAVTIHSCSSSGSQYFRIIEDLITLLGYMQTSKVRRTQEMALALQFRVIQAAIDFIKTTANQDPQKSGSYVHVPTSPHHALQQKRKSIAGRRRFTLAQSDSLLTRMRSVASDELTQMMQRRMSQENPIRASETELIQRMQRLVVLAVNRLIYQDISHDFFDLLNFPESPDHSMSMPTAGDQTSDENGSTPSTPIPAGNIKSFRKDILRLMMEGIKIGLGNTGRGGAPRQQWRRILWSCRDTFRVQIGRMLVHTLSPELPLEDRKEALEFVHEQNYSEILRESLSPGLEHGPKLALYLYELLHDHKDALSREEQAAGNTFMTALKLCGHRCIPPSAPAKPDLLKAIKEEQLKYESEARTSKGAWEKKLSNTQRSLLQRLDGKSKDISKIAADITQNVSLRQGMERKKVMQHIRGLYKTDLSASRHWQELVQQLTHDRAVWYDQSSYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLKDRRKLDDSIKAPLAFLFEDKTHSSSSIVKDKATSEPIRFTRRCVSVAPSRETAGELLLGKSGMYFVEENVPETHDNQSPHGETEPASFSWTYEEIKEVHKRWWQLRDNAMEIFLTNGRTLLLAFDNTKFRDDVYHNILTSDLPNLLEYGNISALTHLWSSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILKDYTNETLDLQEASIYRNLSKPIAVQSKEKEDRYVDNYRYLEEEYKKGKREDDPMPPVQPYHYGSHYSNSGTVLHFLVRLPPFTKMFLAYQDQSFDIPDRTFHSMNTTWRLSSYESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNSERVNHVNLPPWARNDPRLFILIHRQALESDQVSQTLCQWIDLVFGLKQKGKAAVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFSASHISRAGTKLLSEAELPAAMGLLVQLAFRESREQPRDSGYPSPLPWIKGLKWGEYVGSPSAPDPVVCFSQPHGERFGSLLALPTRAICGLSRKFCLMMIYSKEQGVRSMHSTDIQWSAILSWGYTDNMLRLKSKQSEPPINFIQCSPLHQVSSCAWVPDSCQLFTGSKCGVITAYSNRFTTTMPTEMEVESQVDLYGHTAEVTGLFVCKPYSILISVSRDGTCILWDLNRLCYVQSLSGHKSPVNAVSASETTGDIATVCDSVGGGSDLRLWTINGDLIGHVHCREIICSVAFSNQPEGVSVNVIAGGLENGVVRLWSTWDLKPVREITFPKSNRPIISLTFSCDGHHLYTANSEGTVIAWCRRDQQRMKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_021472744.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472744.1

ORF Insert Sequence:

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTTAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACGC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA GCTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTCGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTC CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAAACAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTATTGAG CCATCACAAC CTCACCTGAG ACTTTTTAGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGAAGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGTCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTA 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTGGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAACGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAAAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGCACCG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCAGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CCGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGACCA GTCCAGTGTT CCAGAGGCAG TTCTTCAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCTTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTG 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCCGAAGT CATTGAGGAG AAACCTGACA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACT TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CGGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGATTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
TCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CTGGCTCCAT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGTGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTA AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCTTTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
CTCGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAT 
CTGCTGCGGC TGGTTTATAA TTTCCTGTTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTGTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTACT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC ATGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
CGTAGACGCT TCACACTGGC CCAGTCGGAC TCTCTGCTGA CCCGCATGCG TTCTGTAGCC 
AGTGATGAGC TGACACAGAT GATGCAGCGC AGGATGAGTC AAGAAAACCC CATCAGAGCC 
AGCGAGACCG AGCTCATCCA GAGAATGCAG AGACTGGTGG TTCTGGCTGT CAACAGACTC 
ATTTATCAAG ACATCAGCCA TGATTTCTTT GACCTGCTGA ACTTCCCTGA GTCTCCTGAT 
CACTCGATGT CCATGCCAAC AGCAGGAGAT CAGACATCTG ATGAGAACGG CTCCACTCCG 
TCTACCCCGA TCCCAGCCGG AAACATCAAG AGCTTCCGGA AGGACATTCT CAGACTGATG 
ATGGAGGGCA TAAAGATCGG CCTGGGCAAC ACTGGTCGTG GCGGCGCTCC TCGTCAGCAG 
TGGCGGCGAA TCCTCTGGTC CTGCCGAGAC ACGTTCAGAG TGCAGATCGG CCGCATGCTG 
GTGCACACCC TCAGTCCTGA ACTTCCTCTG GAGGACAGGA AGGAGGCGCT AGAGTTCGTC 
CATGAGCAGA ACTACTCAGA GATCCTCAGA GAAAGCCTCA GCCCTGGCCT CGAGCATGGG 
CCCAAGCTGG CTCTGTATCT GTACGAGCTG CTGCACGATC ACAAAGACGC CCTGAGTAGA 
GAAGAGCAGG CGGCAGGAAA CACCTTCATG ACGGCTCTGA AACTCTGCGG ACACCGCTGC 
ATCCCACCCA GCGCTCCAGC CAAACCTGAC CTCCTGAAAG CCATCAAAGA GGAGCAACTG 
AAGTATGAAT CAGAGGCAAG AACCAGCAAA GGAGCCTGGG AAAAGAAGCT GTCCAACACA 
CAGAGGAGCC TGTTGCAGAG GCTGGACGGC AAGTCGAAGG ACATCTCAAA GATTGCGGCG 
GACATCACTC AGAACGTGTC TCTCAGGCAG GGCATGGAGA GGAAGAAGGT CATGCAGCAC 
ATCAGAGGCC TGTATAAGAC TGACCTGAGC GCTAGTAGAC ACTGGCAGGA GCTCGTCCAG 
CAGCTCACAC ACGACCGGGC GGTGTGGTAC GATCAGTCCT CTTATCCCAC TTCCTGGCAG 
CTGGACCCCA CCGAGGGCCC GAACCGAGAG CGGAGACGCC TCCAGCGCTG CTACCTGACC 
ATCCCCAACA AATATCTGCT CAAAGACCGT CGCAAACTAG ACGATTCCAT TAAGGCTCCT 
CTGGCCTTCC TGTTTGAGGA CAAGACTCAC TCCTCTTCAT CTATAGTGAA GGATAAAGCC 
ACCAGTGAGC CCATCAGGTT CACAAGAAGG TGCGTAAGTG TGGCTCCCTC CAGAGAGACA 
GCAGGGGAGC TCTTACTGGG AAAATCTGGG ATGTATTTTG TGGAGGAAAA TGTTCCTGAG 
ACTCACGATA ACCAGAGTCC TCATGGAGAG ACGGAGCCGG CCTCTTTCTC CTGGACGTAT 
GAGGAGATAA AGGAGGTTCA CAAGCGCTGG TGGCAGCTCA GAGACAACGC CATGGAGATC 
TTCCTGACCA ATGGAAGGAC ACTGCTGCTG GCCTTTGACA ACACAAAGTT TCGTGATGAC 
GTCTACCACA ACATCCTGAC CTCAGATCTG CCCAACCTGC TGGAGTATGG AAACATCAGC 
GCCCTCACGC ACCTCTGGAG CTCAGGACAG ATCACCAACT TTGAGTACCT GACGCATCTC 
AACAAGCACG CGGGACGCTC CTTCAATGAC CTCATGCAGT ATCCCGTCTT CCCCTTCATC 
CTCAAAGATT ACACCAATGA GACGCTCGAC CTGCAGGAGG CCTCTATTTA CAGGAACCTC 
AGCAAGCCCA TTGCAGTGCA GTCTAAAGAG AAGGAAGACC GTTATGTGGA CAATTACAGG 
TATCTGGAGG AGGAGTATAA GAAAGGCAAG AGAGAGGACG ACCCTATGCC CCCTGTCCAG 
CCTTATCACT ACGGCTCTCA TTACTCCAAC AGCGGCACCG TCCTGCACTT CCTGGTCCGA 
CTGCCTCCTT TTACCAAAAT GTTTCTGGCA TATCAAGACC AGAGCTTTGA TATTCCTGAC 
CGAACCTTCC ACTCCATGAA CACCACGTGG CGTCTGTCGT CCTACGAGTC CATGACTGAT 
GTCAAAGAGC TCATCCCAGA GTTCTTCTAC CTGCCCGAGT TCCTGGTCAA CAGAGAAGGT 
TTTGACTTTG GCGTGCGGCA GAACAGCGAG CGGGTCAACC ATGTGAATCT GCCGCCGTGG 
GCCAGAAATG ACCCGCGACT CTTCATCCTC ATCCACAGAC AGGCTCTGGA GTCCGATCAA 
GTCTCGCAGA CGCTCTGCCA GTGGATCGAC CTGGTGTTTG GACTCAAGCA GAAAGGCAAA 
GCTGCGGTCC AGGCCATCAA CGTCTTTCAT CCTGCAACGT ACTTTGGTAT GGACGTGTCT 
GCAGTTGAAG ACCCTGTGCA GCGGCGGGCT CTAGAAACCA TGATCAAGAC GTACGGACAA 
ACACCCAGGC AGCTGTTCAG CGCTTCTCAC ATCAGCAGAG CCGGAACCAA ACTGCTGTCA 
GAGGCTGAGC TGCCCGCCGC CATGGGTCTG CTGGTGCAGC TGGCCTTCAG AGAGAGCCGA 
GAACAGCCCC GGGACAGCGG CTACCCGAGT CCTCTGCCGT GGATAAAGGG CTTGAAATGG 
GGCGAGTATG TGGGTTCACC CAGTGCTCCG GACCCTGTGG TCTGTTTCAG TCAACCTCAC 
GGCGAACGCT TCGGCTCCCT GCTGGCACTG CCCACACGTG CCATCTGCGG CCTCTCACGC 
AAGTTCTGCC TCATGATGAT CTACAGCAAA GAGCAAGGTG TGAGGAGCAT GCACAGCACT 
GACATACAGT GGTCTGCTAT TCTGAGCTGG GGTTACACCG ACAACATGCT GCGTCTGAAG 
AGCAAACAGA GCGAACCACC AATCAACTTC ATCCAGTGCT CACCGCTGCA CCAGGTGAGC 
AGTTGTGCCT GGGTGCCGGA CAGTTGTCAG CTGTTTACAG GCAGCAAGTG TGGAGTCATT 
ACCGCCTACA GCAACCGCTT CACCACCACC ATGCCCACAG AGATGGAGGT GGAGTCGCAG 
GTTGATCTGT ACGGACACAC AGCCGAGGTC ACGGGTCTGT TCGTCTGTAA ACCCTACAGT 
ATCCTCATCA GTGTGAGTCG GGACGGAACC TGCATACTCT GGGATCTCAA CAGGTTGTGT 
TACGTACAGA GTCTCAGCGG TCACAAGAGT CCAGTGAACG CGGTGTCAGC CAGCGAGACC 
ACCGGAGACA TCGCCACCGT CTGCGACTCA GTGGGTGGAG GAAGTGACCT GCGGCTGTGG 
ACCATTAATG GAGATCTGAT TGGTCACGTT CACTGTCGTG AGATCATCTG TTCAGTGGCT 
TTCTCCAATC AGCCGGAGGG AGTTTCAGTC AATGTGATCG CAGGCGGACT GGAGAACGGG 
GTGGTCAGGT TGTGGAGCAC GTGGGACCTG AAGCCAGTCA GAGAGATCAC GTTCCCAAAG 
TCCAACAGAC CTATCATAAG TCTGACGTTC TCATGTGACG GTCATCATCT ATACACGGCT 
AACAGTGAGG GCACGGTGAT CGCGTGGTGT CGGCGAGACC AGCAGCGCAT GAAGCTGCCC 
ATGTTTTACT CTTTCCTCAG CAGTTACGCC GCAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lyst ( XM_009307601.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305876.1 Danio rerio lysosomal trafficking regulator (lyst), transcript variant X2, mRNA.

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CloneID	ODa48897
Clone ID Related Accession (Same CDS sequence)	XM_009307601.2 , XM_009307601.3
Accession Version	XM_009307601.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	lysosomal-trafficking regulator isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307601.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTCAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
CGTAGACGCT TCACACTGGC CCAGTCGGAC TCTCTGCTGA CCCGCATGCG TTCTGTAGCC 
AGTGATGAGC TGACACAGAT GATGCAGCGC AGGATGAGTC AAGAAAACCC CATCAGAGCC 
AGCGAGACCG AGCTCATCCA GAGAATGCAG AGACTGGTGG TCCTGGCTGT CAACAGACTC 
ATTTATCAAG ACATCAGCCA TGATTTCTTT GACCTGCTGA ACTTCCCTGA GTCTCCTGAT 
CACTCGATGT CCATGCCAAC AGCAGGAGAT CAGACATCTG ATGAGAACGG CTCCACTCCG 
TCTACCCCGA TCCCAGCCGG AAACATCAAG AGCTTCCGGA AGGACATTCT CAGACTGATG 
ATGGAGGGCA TAAAGATCGG CCTGGGCAGC ACTGGTCGTG GCGGCGCTCC TCGTCAGCAG 
TGGCGGCGAA TCCTCTGGTC CTGCCGAGAC ACATTCAGAG TGCAGATCGG CCGCATGCTG 
GTGCACACGC TCAGTCCTGA ACTTCCTCTG GAGGACAGGA AGGAGGCGCT AGAGTTCGTC 
CATGAGCAGA GCTACTCCGA GATCCTCAGA GAAAGCCTCA GCCCCGGCCT CGAGCATGGG 
CCCAAGCTGG CTCTGTATCT GTACGAGCTG CTGCACGATC ACAAAGACGC CCTGAGTAGA 
GAAGAGCAGG CGGCAGGAAA CACCTTCATG ACGGCTCTGA AACTCTGCGG ACACCGCTGC 
ATCCCACCCA GCGCTCCAGC CAAACCCGAC CTCCTGAAAG CCATCAAAGA GGAGCAACTA 
AAGTATGAAT CAGAGGCAAG AACCAGCAAA GGAGCCTGGG AAAAGAAGCT GTCCAACACA 
CAGAGGAGCC TGTTGCAGAG GCTGGATGGC AAGTCGAAGG ACATCTCGAA GATCGCGGCG 
GACATCACTC AGAACGTGTC TCTCAGGCAG GGCATGGAGA GGAAGAAGGT CATGCAGCAC 
ATTAGAGGCC TGTATAAGAC TGACCTGAGC GCTAGTCGAC ACTGGCAGGA GCTCGTCCAG 
CAGCTCACAC ACGACCGGGC GGTGTGGTAC GATCAGTCCT CTTATCCCAC TTCCTGGCAG 
CTGGACCCCA CCGAGGGCCC GAACCGAGAG CGGAGACGCC TCCAGCGCTG CTACCTGACC 
ATCCCCAACA AATATCTGCT CAAAGACCGT CGCAAACTAG ATGATTCCAT TAAGGCTCCT 
CTGGCCTTCC TGTTTGAGGA CAAGACTCAC TCCTCTTCAT CTATAGTGAA GGATAAAGCC 
ACCAGTGAGC CCATCAGGTT CACAAGAAGG TGCGTAAGTG TGGCTCCCTC CAGAGAGACA 
GCAGGAGAGC TCTTACTGGG AAAATCTGGG ATGTATTTTG TGGAGGAAAA TGTTCCTGAG 
ACTCACGATA ACCAGAGTCC TCATGGAGAG ACGGAGCCGG CCTCTTTCTC CTGGACGTAT 
GAGGAGATAA AGGAGGTTCA CAAGCGCTGG TGGCAGCTCA GAGACAACGC CATGGAGATC 
TTCCTGACCA ATGGAAGGAC ACTGCTGCTA GCCTTTGACA ACACAAAGTT TCGTGATGAC 
GTCTACCACA ACATCCTGAC CTCAGATCTG CCCAACCTGC TGGAGTATGG AAACATCAGC 
GCCCTCACGC ACCTCTGGAG CTCAGGACAG ATCACCAACT TTGAGTACCT GACGCATCTC 
AACAAGCACG CGGGACGCTC CTTCAATGAC CTCATGCAGT ATCCCGTCTT CCCCTTCATC 
CTCAAAGATT ACACCAATGA GACGCTCGAC CTGCAGGAGG CCTCTATTTA CAGGAACCTC 
AGCAAGCCCA TTGCAGTGCA GTCTAAAGAG AAGGAAGACC GTTATGTGGA CAATTACAGG 
TATCTGGAGG AGGAGTATAA GAAGGGCAAG AGAGAGGACG ACCCTATGCC CCCTGTCCAG 
CCCTATCACT ACGGCTCTCA TTACTCCAAC AGTGGCACCG TCCTGCACTT CCTGGTCCGA 
CTGCCTCCTT TTACCAAAAT GTTTCTGGCA TATCAAGACC AAAGCTTTGA TATTCCTGAC 
CGAACCTTCC ACTCCATGAA CACCACGTGG CGTCTGTCGT CCTACGAGTC CATGACTGAT 
GTCAAAGAGC TCATCCCAGA GTTCTTCTAC CTGCCCGAGT TCCTGGTCAA CAGAGAAGGT 
TTTGACTTTG GCGTGCGGCA GAACAGCGAG CGGGTCAACC ATGTGAATCT GCCGCCGTGG 
GCCAGAAATG ACCCGCGACT CTTCATCCTC ATCCACAGAC AGGCTCTGGA GTCCGATCAA 
GTCTCGCAGA CGCTCTGCCA GTGGATCGAC CTGGTGTTTG GACTCAAGCA GAAAGGCAAA 
GCTGCGGTCC AGGCCATCAA CGTCTTTCAT CCTGCAACGT ACTTTGGTAT GGACGTGTCT 
GCAGTTGAAG ACCCTGTGCA GCGGCGGGCT CTAGAAACCA TGATCAAGAC GTACGGACAA 
ACACCCAGGC AGCTGTTCAG CGCTTCTCAC ATCAGCAGAG CCGGAACCAA ACTGCTGTCA 
GAGGCTGAGC TGCCCGCCGC CATGGGTCTG CTGGTGCAGC TGGCCTTCAG AGAGAGCCGA 
GAACAGCCCC GGGACAGCGG CTACCCGAGT CCTCTGCCGT GGATAAAGGG CTTGAAATGG 
GGCGAGTATG TGGGTTCACC CAGTGCTCCG GACCCTGTGG TCTGTTTCAG TCAACCTCAC 
GGCGAACGCT TCGGCTCCCT GCTGGCACTG CCCACACGTG CCATCTGCGG CCTCTCACGC 
AAGTTCTGCC TCATGATGAT CTACAGCAAA GAGCAAGGTG TGAGGAGCAT GCACAGCACT 
GACATACAGT GGTCTGCTAT TCTGAGCTGG GGTTACACCG ACAACATGCT GCGTCTGAAG 
AGCAAACAGA GCGAACCACC AATCAACTTC ATCCAGTGCT CACCGCTGCA CCAGGTGAGC 
AGTTGTGCCT GGGTGCCGGA CAGTTGTCAG CTGTTTACAG GCAGCAAGTG TGGAGTCATT 
ACCGCCTACA GCAACCGCTT CACCACCACC ATGCCCACAG AGATGGAGGT GGAGTCGCAG 
GTTGATCTGT ACGGACACAC AGCCGAGGTC ACGGGTCTGT TCGTCTGTAA ACCCTACAGT 
ATCCTCATCA GTGTGAGTCG GGACGGAACC TGCATACTCT GGGATCTCAA CAGGTTGTGT 
TACGTACAGA GTCTCAGCGG TCACAAGAGT CCAGTGAACG CGGTGTCAGC CAGCGAGACC 
ACCGGAGACA TCGCCACCGT CTGCGACTCA GTGGGTGGAG GAAGTGACCT GCGGCTGTGG 
ACCATTAATG GAGATCTGAT TGGTCACGTT CACTGTCGTG AGATCATCTG TTCAGTGGCT 
TTCTCCAATC AGCCGGAGGG AGTTTCAGTC AATGTGATCG CAGGCGGACT GGAGAACGGG 
GTGGTCAGGT TGTGGAGCAC GTGGGACCTG AAGCCAGTCA GAGAGATCAC GTTCCCAAAG 
TCCAACAGAC CTATCATAAG TCTGACGTTC TCATGTGACG GTCATCATCT ATACACGGCT 
AACAGTGAGG GCACGGTGAT CGCGTGGTGT CGGCGAGACC AGCAGCGCAT GAAGCTGCCC 
ATGTTTTACT CTTTCCTCAG CAGTTACGCC GCAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305876.1
CDS	198..11696
Translation	MASSHLADLSQAWENYMDCRTQGADLQVSLQCLESFLCTFHCVRHLSDPRAKDALKFCSDMSGASNTLAREFLTDVHQLCSAVAQRAEDEEESHMVALGEYLVRGRGFLLLSTLESVIDQELTCREELLTLLLSLLPLVWKIPVQEEKAPDFTLPSLLEVFLCQEGRTISQKAGQDPCDVQNSTKRLSGSWKSRRSRRTAQRYSVRDARKSQLSTSDSEANSDDKTTNTGSKHRRFHSTAIHVSCQLHQSNNSSQPAPQHPTLDAMGTFEPSQPHLRLFGSHTMDPNMLSDPTTLSIFNRMENSPFDLCHVLLSLLEKVCKFDMTINHNPGLAVSVVPTLTEILTDFGDCCGPSGGGGGGIDELAGGWTEEPIALVQRMLLRTILHLMFVDVGQNETLPDNLRRSLTDLLRATLKIRTCLERQADPFAPRPKKTLQEIQEDFSFSRYRHRALLLPELLQGVLQLLLGCLQASAPNPFFFSQSLELVHEFVQHRGLELFEVTALRLEALAEAHNSEISNEASEHLRCLIADVLKIISAVKRAKSEQLHQSVCARRRHRRCEYSHFLHHHRDLSGLPVSAFKQAARRNPFEEDSKGNDGEVRYPERCCCLAACAHQCLRLFQRLSPSGPAVLQVLAGVQAVGICCCMDPRSVIAPMLQAFQAPGLRSYQSHVLSVLSRLVLEQLGGGQPSEKAKLASCNICTLDSSQLPGLEETLQGCGPKKELNLSTSPSPSYRSQGILPSGGAEDILWKWDALEAYQELVFGEDWHLSQQIASHVCQLTLRGNAVVQWQLYTHIFNPVLQRGVELTHHAQQLGVSTTCSQVCSYHTHCLPVEVLLVYLQTLPALLKSSRVVRDLFLSCNGLNQVTELIYLDCTRPWALKVFETLILNAWRNHTESAVHELERAEAQEREAVLGLVEELSTRGTGDAPQSLTKFYEGLKEACPPPKGKSETSEFSGVGRNRGEAHLNTINLFLCVAFLCVSKEADSDRDSANDSEDTSGYDSTASEPLGGRLPCLSPDSVALPSKEQVRRAADVWSVCRWVYMASPVFQRQFFRLGGLDVCSRLMTMVIQKLTSKNKDCKFKKKRESKGKSSPSREDSPTRCITGHFDNMVTNAGTAETQHPELSDTVSLSQTESKSSDPMHRLEEEWPFQSIRLLEALLAICLHSANSALQRLEPDLSFQLQSVEETLCEVRDQLSRSGVVNSELAVPLFDSLLRVALAEMFCSPEVIEEKPDKKCAVSEATPPAGDLSEDVDEAHVSAVQPLGEEEGYDADSESNPEDSTRQEEGLKVEAGVSLRECTLGMAGVPQGSVRGRLLFPEICLMELQLLASGSPDLEVLGHVLQSLLAAVKGHHNNAAQLYQQGGVKVILTGFPNILSQSDASYKECQTVLMELLVAMVSDRITAEELALLTRLFLEKTPPTEILLRGVLQIVEANTDTEPLYFMSFPMIPGSGGPGLSPSPGTRPHGATAGKGGVNTILRGKLPLGRGEADMSRPGHLRSSPWHAAPFHLPLVGQNCWPHMASGFSASLWVRAGKDEDGRSKKEDEVCVAEESPHGSAHVTEQQTERCLLHVLSMGSKALMLQVWVNISTRAFTFRICIDPNDEMKAGLLAQAESGDEVIPAGRWQHLALTYTQQPEGKKNIHGQLSLWVCGIRKCDVSLDFTLPRKSSLSSDSNKTYCMLGHYQHSAEEQASPSARWDMGTLMLFNGSRIDTTEAFYLYASGPDLTSIMPCKYGKPHGTLSKYVTQEGLQCEQIRELLMRNADVDISPLVESLAVVYTPSTPSLYTIYEPVIRLKGQAKSAVVSQRPFSAKEVQSSILESPFLRTMMPCQAFGLHSALHKIGGTGSVVFLFARAVELCDCEKTQALALQVLLSLVKNNQHRTHEMECYHGYSMIHQVLIKPKCIVGYHILKTLLDGCCSGSILTIGEDGQFHLDAESTVVVQDLRLLSEILLDWKIWAKAQCGVWETLLTALEILIRVRHPQQMFNIRQLLKAQVVHRFLLACQVLQEHRDEYLTSIPQEVCLSFVKIIQEVLGSPPDMDLLRLVYNFLLAVHPPTNTYVCHTPTSFYFSLHIDGKLYQEKVQSMMYLRHSSSGGKSACSSVVSLSPTVFTEMPAEVRHTSSIEHEDDLSLRPRSTGPSPHSSPALTPRLTHGRVTGTTEPEGGASLSTQQALGSTETLKRAGDEQLLSSCESAKTICDSPIAGADEEGSIMGNRTPSISVEEEQDGPAGSPVESEEHFDWTSEEAPRRPDSLKGIQSFQRSHSNLASLGLAFPAQNGSLTIARWPTVADRAAPPDDWESYTYSPGYDRHSKADSSNDRSSAEDCLVLICCGLYDLLRGVLLLLPDLLLEEVMDKLIQPDALLVLVNHPSPLIQQGVMKLLDAYFSRAQKEQKEKFLKNHGFSLLANQLYQHQGSQGLLECFLEMLFGRPVGLEEDLDLEDMENISPFRKRCIIPVLGLLENSLYENSLVHNGLCLLLQLLNACPKLADILLDHGLLYVLFNTLSTLNGLENGIPPNDYRLVVCDIQQLLVAVTIHSCSSSGSQYFRIIEDLITLLGYMQTSKVRRTQEMALALQFRVIQAAIDFIKTTANQDPQKSGSYVHVPTSPHHALQQKRKSIAGRRRFTLAQSDSLLTRMRSVASDELTQMMQRRMSQENPIRASETELIQRMQRLVVLAVNRLIYQDISHDFFDLLNFPESPDHSMSMPTAGDQTSDENGSTPSTPIPAGNIKSFRKDILRLMMEGIKIGLGSTGRGGAPRQQWRRILWSCRDTFRVQIGRMLVHTLSPELPLEDRKEALEFVHEQSYSEILRESLSPGLEHGPKLALYLYELLHDHKDALSREEQAAGNTFMTALKLCGHRCIPPSAPAKPDLLKAIKEEQLKYESEARTSKGAWEKKLSNTQRSLLQRLDGKSKDISKIAADITQNVSLRQGMERKKVMQHIRGLYKTDLSASRHWQELVQQLTHDRAVWYDQSSYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLKDRRKLDDSIKAPLAFLFEDKTHSSSSIVKDKATSEPIRFTRRCVSVAPSRETAGELLLGKSGMYFVEENVPETHDNQSPHGETEPASFSWTYEEIKEVHKRWWQLRDNAMEIFLTNGRTLLLAFDNTKFRDDVYHNILTSDLPNLLEYGNISALTHLWSSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILKDYTNETLDLQEASIYRNLSKPIAVQSKEKEDRYVDNYRYLEEEYKKGKREDDPMPPVQPYHYGSHYSNSGTVLHFLVRLPPFTKMFLAYQDQSFDIPDRTFHSMNTTWRLSSYESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNSERVNHVNLPPWARNDPRLFILIHRQALESDQVSQTLCQWIDLVFGLKQKGKAAVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFSASHISRAGTKLLSEAELPAAMGLLVQLAFRESREQPRDSGYPSPLPWIKGLKWGEYVGSPSAPDPVVCFSQPHGERFGSLLALPTRAICGLSRKFCLMMIYSKEQGVRSMHSTDIQWSAILSWGYTDNMLRLKSKQSEPPINFIQCSPLHQVSSCAWVPDSCQLFTGSKCGVITAYSNRFTTTMPTEMEVESQVDLYGHTAEVTGLFVCKPYSILISVSRDGTCILWDLNRLCYVQSLSGHKSPVNAVSASETTGDIATVCDSVGGGSDLRLWTINGDLIGHVHCREIICSVAFSNQPEGVSVNVIAGGLENGVVRLWSTWDLKPVREITFPKSNRPIISLTFSCDGHHLYTANSEGTVIAWCRRDQQRMKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_009307601.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307601.2

ORF Insert Sequence:

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTCAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
CGTAGACGCT TCACACTGGC CCAGTCGGAC TCTCTGCTGA CCCGCATGCG TTCTGTAGCC 
AGTGATGAGC TGACACAGAT GATGCAGCGC AGGATGAGTC AAGAAAACCC CATCAGAGCC 
AGCGAGACCG AGCTCATCCA GAGAATGCAG AGACTGGTGG TCCTGGCTGT CAACAGACTC 
ATTTATCAAG ACATCAGCCA TGATTTCTTT GACCTGCTGA ACTTCCCTGA GTCTCCTGAT 
CACTCGATGT CCATGCCAAC AGCAGGAGAT CAGACATCTG ATGAGAACGG CTCCACTCCG 
TCTACCCCGA TCCCAGCCGG AAACATCAAG AGCTTCCGGA AGGACATTCT CAGACTGATG 
ATGGAGGGCA TAAAGATCGG CCTGGGCAGC ACTGGTCGTG GCGGCGCTCC TCGTCAGCAG 
TGGCGGCGAA TCCTCTGGTC CTGCCGAGAC ACATTCAGAG TGCAGATCGG CCGCATGCTG 
GTGCACACGC TCAGTCCTGA ACTTCCTCTG GAGGACAGGA AGGAGGCGCT AGAGTTCGTC 
CATGAGCAGA GCTACTCCGA GATCCTCAGA GAAAGCCTCA GCCCCGGCCT CGAGCATGGG 
CCCAAGCTGG CTCTGTATCT GTACGAGCTG CTGCACGATC ACAAAGACGC CCTGAGTAGA 
GAAGAGCAGG CGGCAGGAAA CACCTTCATG ACGGCTCTGA AACTCTGCGG ACACCGCTGC 
ATCCCACCCA GCGCTCCAGC CAAACCCGAC CTCCTGAAAG CCATCAAAGA GGAGCAACTA 
AAGTATGAAT CAGAGGCAAG AACCAGCAAA GGAGCCTGGG AAAAGAAGCT GTCCAACACA 
CAGAGGAGCC TGTTGCAGAG GCTGGATGGC AAGTCGAAGG ACATCTCGAA GATCGCGGCG 
GACATCACTC AGAACGTGTC TCTCAGGCAG GGCATGGAGA GGAAGAAGGT CATGCAGCAC 
ATTAGAGGCC TGTATAAGAC TGACCTGAGC GCTAGTCGAC ACTGGCAGGA GCTCGTCCAG 
CAGCTCACAC ACGACCGGGC GGTGTGGTAC GATCAGTCCT CTTATCCCAC TTCCTGGCAG 
CTGGACCCCA CCGAGGGCCC GAACCGAGAG CGGAGACGCC TCCAGCGCTG CTACCTGACC 
ATCCCCAACA AATATCTGCT CAAAGACCGT CGCAAACTAG ATGATTCCAT TAAGGCTCCT 
CTGGCCTTCC TGTTTGAGGA CAAGACTCAC TCCTCTTCAT CTATAGTGAA GGATAAAGCC 
ACCAGTGAGC CCATCAGGTT CACAAGAAGG TGCGTAAGTG TGGCTCCCTC CAGAGAGACA 
GCAGGAGAGC TCTTACTGGG AAAATCTGGG ATGTATTTTG TGGAGGAAAA TGTTCCTGAG 
ACTCACGATA ACCAGAGTCC TCATGGAGAG ACGGAGCCGG CCTCTTTCTC CTGGACGTAT 
GAGGAGATAA AGGAGGTTCA CAAGCGCTGG TGGCAGCTCA GAGACAACGC CATGGAGATC 
TTCCTGACCA ATGGAAGGAC ACTGCTGCTA GCCTTTGACA ACACAAAGTT TCGTGATGAC 
GTCTACCACA ACATCCTGAC CTCAGATCTG CCCAACCTGC TGGAGTATGG AAACATCAGC 
GCCCTCACGC ACCTCTGGAG CTCAGGACAG ATCACCAACT TTGAGTACCT GACGCATCTC 
AACAAGCACG CGGGACGCTC CTTCAATGAC CTCATGCAGT ATCCCGTCTT CCCCTTCATC 
CTCAAAGATT ACACCAATGA GACGCTCGAC CTGCAGGAGG CCTCTATTTA CAGGAACCTC 
AGCAAGCCCA TTGCAGTGCA GTCTAAAGAG AAGGAAGACC GTTATGTGGA CAATTACAGG 
TATCTGGAGG AGGAGTATAA GAAGGGCAAG AGAGAGGACG ACCCTATGCC CCCTGTCCAG 
CCCTATCACT ACGGCTCTCA TTACTCCAAC AGTGGCACCG TCCTGCACTT CCTGGTCCGA 
CTGCCTCCTT TTACCAAAAT GTTTCTGGCA TATCAAGACC AAAGCTTTGA TATTCCTGAC 
CGAACCTTCC ACTCCATGAA CACCACGTGG CGTCTGTCGT CCTACGAGTC CATGACTGAT 
GTCAAAGAGC TCATCCCAGA GTTCTTCTAC CTGCCCGAGT TCCTGGTCAA CAGAGAAGGT 
TTTGACTTTG GCGTGCGGCA GAACAGCGAG CGGGTCAACC ATGTGAATCT GCCGCCGTGG 
GCCAGAAATG ACCCGCGACT CTTCATCCTC ATCCACAGAC AGGCTCTGGA GTCCGATCAA 
GTCTCGCAGA CGCTCTGCCA GTGGATCGAC CTGGTGTTTG GACTCAAGCA GAAAGGCAAA 
GCTGCGGTCC AGGCCATCAA CGTCTTTCAT CCTGCAACGT ACTTTGGTAT GGACGTGTCT 
GCAGTTGAAG ACCCTGTGCA GCGGCGGGCT CTAGAAACCA TGATCAAGAC GTACGGACAA 
ACACCCAGGC AGCTGTTCAG CGCTTCTCAC ATCAGCAGAG CCGGAACCAA ACTGCTGTCA 
GAGGCTGAGC TGCCCGCCGC CATGGGTCTG CTGGTGCAGC TGGCCTTCAG AGAGAGCCGA 
GAACAGCCCC GGGACAGCGG CTACCCGAGT CCTCTGCCGT GGATAAAGGG CTTGAAATGG 
GGCGAGTATG TGGGTTCACC CAGTGCTCCG GACCCTGTGG TCTGTTTCAG TCAACCTCAC 
GGCGAACGCT TCGGCTCCCT GCTGGCACTG CCCACACGTG CCATCTGCGG CCTCTCACGC 
AAGTTCTGCC TCATGATGAT CTACAGCAAA GAGCAAGGTG TGAGGAGCAT GCACAGCACT 
GACATACAGT GGTCTGCTAT TCTGAGCTGG GGTTACACCG ACAACATGCT GCGTCTGAAG 
AGCAAACAGA GCGAACCACC AATCAACTTC ATCCAGTGCT CACCGCTGCA CCAGGTGAGC 
AGTTGTGCCT GGGTGCCGGA CAGTTGTCAG CTGTTTACAG GCAGCAAGTG TGGAGTCATT 
ACCGCCTACA GCAACCGCTT CACCACCACC ATGCCCACAG AGATGGAGGT GGAGTCGCAG 
GTTGATCTGT ACGGACACAC AGCCGAGGTC ACGGGTCTGT TCGTCTGTAA ACCCTACAGT 
ATCCTCATCA GTGTGAGTCG GGACGGAACC TGCATACTCT GGGATCTCAA CAGGTTGTGT 
TACGTACAGA GTCTCAGCGG TCACAAGAGT CCAGTGAACG CGGTGTCAGC CAGCGAGACC 
ACCGGAGACA TCGCCACCGT CTGCGACTCA GTGGGTGGAG GAAGTGACCT GCGGCTGTGG 
ACCATTAATG GAGATCTGAT TGGTCACGTT CACTGTCGTG AGATCATCTG TTCAGTGGCT 
TTCTCCAATC AGCCGGAGGG AGTTTCAGTC AATGTGATCG CAGGCGGACT GGAGAACGGG 
GTGGTCAGGT TGTGGAGCAC GTGGGACCTG AAGCCAGTCA GAGAGATCAC GTTCCCAAAG 
TCCAACAGAC CTATCATAAG TCTGACGTTC TCATGTGACG GTCATCATCT ATACACGGCT 
AACAGTGAGG GCACGGTGAT CGCGTGGTGT CGGCGAGACC AGCAGCGCAT GAAGCTGCCC 
ATGTTTTACT CTTTCCTCAG CAGTTACGCC GCAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lyst ( XM_009307601.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305876.1 Danio rerio lysosomal trafficking regulator (lyst), transcript variant X2, mRNA.

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CloneID	ODa48897
Clone ID Related Accession (Same CDS sequence)	XM_009307601.2 , XM_009307601.3
Accession Version	XM_009307601.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lysosomal-trafficking regulator isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307601.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTCAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
CGTAGACGCT TCACACTGGC CCAGTCGGAC TCTCTGCTGA CCCGCATGCG TTCTGTAGCC 
AGTGATGAGC TGACACAGAT GATGCAGCGC AGGATGAGTC AAGAAAACCC CATCAGAGCC 
AGCGAGACCG AGCTCATCCA GAGAATGCAG AGACTGGTGG TCCTGGCTGT CAACAGACTC 
ATTTATCAAG ACATCAGCCA TGATTTCTTT GACCTGCTGA ACTTCCCTGA GTCTCCTGAT 
CACTCGATGT CCATGCCAAC AGCAGGAGAT CAGACATCTG ATGAGAACGG CTCCACTCCG 
TCTACCCCGA TCCCAGCCGG AAACATCAAG AGCTTCCGGA AGGACATTCT CAGACTGATG 
ATGGAGGGCA TAAAGATCGG CCTGGGCAGC ACTGGTCGTG GCGGCGCTCC TCGTCAGCAG 
TGGCGGCGAA TCCTCTGGTC CTGCCGAGAC ACATTCAGAG TGCAGATCGG CCGCATGCTG 
GTGCACACGC TCAGTCCTGA ACTTCCTCTG GAGGACAGGA AGGAGGCGCT AGAGTTCGTC 
CATGAGCAGA GCTACTCCGA GATCCTCAGA GAAAGCCTCA GCCCCGGCCT CGAGCATGGG 
CCCAAGCTGG CTCTGTATCT GTACGAGCTG CTGCACGATC ACAAAGACGC CCTGAGTAGA 
GAAGAGCAGG CGGCAGGAAA CACCTTCATG ACGGCTCTGA AACTCTGCGG ACACCGCTGC 
ATCCCACCCA GCGCTCCAGC CAAACCCGAC CTCCTGAAAG CCATCAAAGA GGAGCAACTA 
AAGTATGAAT CAGAGGCAAG AACCAGCAAA GGAGCCTGGG AAAAGAAGCT GTCCAACACA 
CAGAGGAGCC TGTTGCAGAG GCTGGATGGC AAGTCGAAGG ACATCTCGAA GATCGCGGCG 
GACATCACTC AGAACGTGTC TCTCAGGCAG GGCATGGAGA GGAAGAAGGT CATGCAGCAC 
ATTAGAGGCC TGTATAAGAC TGACCTGAGC GCTAGTCGAC ACTGGCAGGA GCTCGTCCAG 
CAGCTCACAC ACGACCGGGC GGTGTGGTAC GATCAGTCCT CTTATCCCAC TTCCTGGCAG 
CTGGACCCCA CCGAGGGCCC GAACCGAGAG CGGAGACGCC TCCAGCGCTG CTACCTGACC 
ATCCCCAACA AATATCTGCT CAAAGACCGT CGCAAACTAG ATGATTCCAT TAAGGCTCCT 
CTGGCCTTCC TGTTTGAGGA CAAGACTCAC TCCTCTTCAT CTATAGTGAA GGATAAAGCC 
ACCAGTGAGC CCATCAGGTT CACAAGAAGG TGCGTAAGTG TGGCTCCCTC CAGAGAGACA 
GCAGGAGAGC TCTTACTGGG AAAATCTGGG ATGTATTTTG TGGAGGAAAA TGTTCCTGAG 
ACTCACGATA ACCAGAGTCC TCATGGAGAG ACGGAGCCGG CCTCTTTCTC CTGGACGTAT 
GAGGAGATAA AGGAGGTTCA CAAGCGCTGG TGGCAGCTCA GAGACAACGC CATGGAGATC 
TTCCTGACCA ATGGAAGGAC ACTGCTGCTA GCCTTTGACA ACACAAAGTT TCGTGATGAC 
GTCTACCACA ACATCCTGAC CTCAGATCTG CCCAACCTGC TGGAGTATGG AAACATCAGC 
GCCCTCACGC ACCTCTGGAG CTCAGGACAG ATCACCAACT TTGAGTACCT GACGCATCTC 
AACAAGCACG CGGGACGCTC CTTCAATGAC CTCATGCAGT ATCCCGTCTT CCCCTTCATC 
CTCAAAGATT ACACCAATGA GACGCTCGAC CTGCAGGAGG CCTCTATTTA CAGGAACCTC 
AGCAAGCCCA TTGCAGTGCA GTCTAAAGAG AAGGAAGACC GTTATGTGGA CAATTACAGG 
TATCTGGAGG AGGAGTATAA GAAGGGCAAG AGAGAGGACG ACCCTATGCC CCCTGTCCAG 
CCCTATCACT ACGGCTCTCA TTACTCCAAC AGTGGCACCG TCCTGCACTT CCTGGTCCGA 
CTGCCTCCTT TTACCAAAAT GTTTCTGGCA TATCAAGACC AAAGCTTTGA TATTCCTGAC 
CGAACCTTCC ACTCCATGAA CACCACGTGG CGTCTGTCGT CCTACGAGTC CATGACTGAT 
GTCAAAGAGC TCATCCCAGA GTTCTTCTAC CTGCCCGAGT TCCTGGTCAA CAGAGAAGGT 
TTTGACTTTG GCGTGCGGCA GAACAGCGAG CGGGTCAACC ATGTGAATCT GCCGCCGTGG 
GCCAGAAATG ACCCGCGACT CTTCATCCTC ATCCACAGAC AGGCTCTGGA GTCCGATCAA 
GTCTCGCAGA CGCTCTGCCA GTGGATCGAC CTGGTGTTTG GACTCAAGCA GAAAGGCAAA 
GCTGCGGTCC AGGCCATCAA CGTCTTTCAT CCTGCAACGT ACTTTGGTAT GGACGTGTCT 
GCAGTTGAAG ACCCTGTGCA GCGGCGGGCT CTAGAAACCA TGATCAAGAC GTACGGACAA 
ACACCCAGGC AGCTGTTCAG CGCTTCTCAC ATCAGCAGAG CCGGAACCAA ACTGCTGTCA 
GAGGCTGAGC TGCCCGCCGC CATGGGTCTG CTGGTGCAGC TGGCCTTCAG AGAGAGCCGA 
GAACAGCCCC GGGACAGCGG CTACCCGAGT CCTCTGCCGT GGATAAAGGG CTTGAAATGG 
GGCGAGTATG TGGGTTCACC CAGTGCTCCG GACCCTGTGG TCTGTTTCAG TCAACCTCAC 
GGCGAACGCT TCGGCTCCCT GCTGGCACTG CCCACACGTG CCATCTGCGG CCTCTCACGC 
AAGTTCTGCC TCATGATGAT CTACAGCAAA GAGCAAGGTG TGAGGAGCAT GCACAGCACT 
GACATACAGT GGTCTGCTAT TCTGAGCTGG GGTTACACCG ACAACATGCT GCGTCTGAAG 
AGCAAACAGA GCGAACCACC AATCAACTTC ATCCAGTGCT CACCGCTGCA CCAGGTGAGC 
AGTTGTGCCT GGGTGCCGGA CAGTTGTCAG CTGTTTACAG GCAGCAAGTG TGGAGTCATT 
ACCGCCTACA GCAACCGCTT CACCACCACC ATGCCCACAG AGATGGAGGT GGAGTCGCAG 
GTTGATCTGT ACGGACACAC AGCCGAGGTC ACGGGTCTGT TCGTCTGTAA ACCCTACAGT 
ATCCTCATCA GTGTGAGTCG GGACGGAACC TGCATACTCT GGGATCTCAA CAGGTTGTGT 
TACGTACAGA GTCTCAGCGG TCACAAGAGT CCAGTGAACG CGGTGTCAGC CAGCGAGACC 
ACCGGAGACA TCGCCACCGT CTGCGACTCA GTGGGTGGAG GAAGTGACCT GCGGCTGTGG 
ACCATTAATG GAGATCTGAT TGGTCACGTT CACTGTCGTG AGATCATCTG TTCAGTGGCT 
TTCTCCAATC AGCCGGAGGG AGTTTCAGTC AATGTGATCG CAGGCGGACT GGAGAACGGG 
GTGGTCAGGT TGTGGAGCAC GTGGGACCTG AAGCCAGTCA GAGAGATCAC GTTCCCAAAG 
TCCAACAGAC CTATCATAAG TCTGACGTTC TCATGTGACG GTCATCATCT ATACACGGCT 
AACAGTGAGG GCACGGTGAT CGCGTGGTGT CGGCGAGACC AGCAGCGCAT GAAGCTGCCC 
ATGTTTTACT CTTTCCTCAG CAGTTACGCC GCAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305876.1
CDS	202..11700
Translation	MASSHLADLSQAWENYMDCRTQGADLQVSLQCLESFLCTFHCVRHLSDPRAKDALKFCSDMSGASNTLAREFLTDVHQLCSAVAQRAEDEEESHMVALGEYLVRGRGFLLLSTLESVIDQELTCREELLTLLLSLLPLVWKIPVQEEKAPDFTLPSLLEVFLCQEGRTISQKAGQDPCDVQNSTKRLSGSWKSRRSRRTAQRYSVRDARKSQLSTSDSEANSDDKTTNTGSKHRRFHSTAIHVSCQLHQSNNSSQPAPQHPTLDAMGTFEPSQPHLRLFGSHTMDPNMLSDPTTLSIFNRMENSPFDLCHVLLSLLEKVCKFDMTINHNPGLAVSVVPTLTEILTDFGDCCGPSGGGGGGIDELAGGWTEEPIALVQRMLLRTILHLMFVDVGQNETLPDNLRRSLTDLLRATLKIRTCLERQADPFAPRPKKTLQEIQEDFSFSRYRHRALLLPELLQGVLQLLLGCLQASAPNPFFFSQSLELVHEFVQHRGLELFEVTALRLEALAEAHNSEISNEASEHLRCLIADVLKIISAVKRAKSEQLHQSVCARRRHRRCEYSHFLHHHRDLSGLPVSAFKQAARRNPFEEDSKGNDGEVRYPERCCCLAACAHQCLRLFQRLSPSGPAVLQVLAGVQAVGICCCMDPRSVIAPMLQAFQAPGLRSYQSHVLSVLSRLVLEQLGGGQPSEKAKLASCNICTLDSSQLPGLEETLQGCGPKKELNLSTSPSPSYRSQGILPSGGAEDILWKWDALEAYQELVFGEDWHLSQQIASHVCQLTLRGNAVVQWQLYTHIFNPVLQRGVELTHHAQQLGVSTTCSQVCSYHTHCLPVEVLLVYLQTLPALLKSSRVVRDLFLSCNGLNQVTELIYLDCTRPWALKVFETLILNAWRNHTESAVHELERAEAQEREAVLGLVEELSTRGTGDAPQSLTKFYEGLKEACPPPKGKSETSEFSGVGRNRGEAHLNTINLFLCVAFLCVSKEADSDRDSANDSEDTSGYDSTASEPLGGRLPCLSPDSVALPSKEQVRRAADVWSVCRWVYMASPVFQRQFFRLGGLDVCSRLMTMVIQKLTSKNKDCKFKKKRESKGKSSPSREDSPTRCITGHFDNMVTNAGTAETQHPELSDTVSLSQTESKSSDPMHRLEEEWPFQSIRLLEALLAICLHSANSALQRLEPDLSFQLQSVEETLCEVRDQLSRSGVVNSELAVPLFDSLLRVALAEMFCSPEVIEEKPDKKCAVSEATPPAGDLSEDVDEAHVSAVQPLGEEEGYDADSESNPEDSTRQEEGLKVEAGVSLRECTLGMAGVPQGSVRGRLLFPEICLMELQLLASGSPDLEVLGHVLQSLLAAVKGHHNNAAQLYQQGGVKVILTGFPNILSQSDASYKECQTVLMELLVAMVSDRITAEELALLTRLFLEKTPPTEILLRGVLQIVEANTDTEPLYFMSFPMIPGSGGPGLSPSPGTRPHGATAGKGGVNTILRGKLPLGRGEADMSRPGHLRSSPWHAAPFHLPLVGQNCWPHMASGFSASLWVRAGKDEDGRSKKEDEVCVAEESPHGSAHVTEQQTERCLLHVLSMGSKALMLQVWVNISTRAFTFRICIDPNDEMKAGLLAQAESGDEVIPAGRWQHLALTYTQQPEGKKNIHGQLSLWVCGIRKCDVSLDFTLPRKSSLSSDSNKTYCMLGHYQHSAEEQASPSARWDMGTLMLFNGSRIDTTEAFYLYASGPDLTSIMPCKYGKPHGTLSKYVTQEGLQCEQIRELLMRNADVDISPLVESLAVVYTPSTPSLYTIYEPVIRLKGQAKSAVVSQRPFSAKEVQSSILESPFLRTMMPCQAFGLHSALHKIGGTGSVVFLFARAVELCDCEKTQALALQVLLSLVKNNQHRTHEMECYHGYSMIHQVLIKPKCIVGYHILKTLLDGCCSGSILTIGEDGQFHLDAESTVVVQDLRLLSEILLDWKIWAKAQCGVWETLLTALEILIRVRHPQQMFNIRQLLKAQVVHRFLLACQVLQEHRDEYLTSIPQEVCLSFVKIIQEVLGSPPDMDLLRLVYNFLLAVHPPTNTYVCHTPTSFYFSLHIDGKLYQEKVQSMMYLRHSSSGGKSACSSVVSLSPTVFTEMPAEVRHTSSIEHEDDLSLRPRSTGPSPHSSPALTPRLTHGRVTGTTEPEGGASLSTQQALGSTETLKRAGDEQLLSSCESAKTICDSPIAGADEEGSIMGNRTPSISVEEEQDGPAGSPVESEEHFDWTSEEAPRRPDSLKGIQSFQRSHSNLASLGLAFPAQNGSLTIARWPTVADRAAPPDDWESYTYSPGYDRHSKADSSNDRSSAEDCLVLICCGLYDLLRGVLLLLPDLLLEEVMDKLIQPDALLVLVNHPSPLIQQGVMKLLDAYFSRAQKEQKEKFLKNHGFSLLANQLYQHQGSQGLLECFLEMLFGRPVGLEEDLDLEDMENISPFRKRCIIPVLGLLENSLYENSLVHNGLCLLLQLLNACPKLADILLDHGLLYVLFNTLSTLNGLENGIPPNDYRLVVCDIQQLLVAVTIHSCSSSGSQYFRIIEDLITLLGYMQTSKVRRTQEMALALQFRVIQAAIDFIKTTANQDPQKSGSYVHVPTSPHHALQQKRKSIAGRRRFTLAQSDSLLTRMRSVASDELTQMMQRRMSQENPIRASETELIQRMQRLVVLAVNRLIYQDISHDFFDLLNFPESPDHSMSMPTAGDQTSDENGSTPSTPIPAGNIKSFRKDILRLMMEGIKIGLGSTGRGGAPRQQWRRILWSCRDTFRVQIGRMLVHTLSPELPLEDRKEALEFVHEQSYSEILRESLSPGLEHGPKLALYLYELLHDHKDALSREEQAAGNTFMTALKLCGHRCIPPSAPAKPDLLKAIKEEQLKYESEARTSKGAWEKKLSNTQRSLLQRLDGKSKDISKIAADITQNVSLRQGMERKKVMQHIRGLYKTDLSASRHWQELVQQLTHDRAVWYDQSSYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLKDRRKLDDSIKAPLAFLFEDKTHSSSSIVKDKATSEPIRFTRRCVSVAPSRETAGELLLGKSGMYFVEENVPETHDNQSPHGETEPASFSWTYEEIKEVHKRWWQLRDNAMEIFLTNGRTLLLAFDNTKFRDDVYHNILTSDLPNLLEYGNISALTHLWSSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILKDYTNETLDLQEASIYRNLSKPIAVQSKEKEDRYVDNYRYLEEEYKKGKREDDPMPPVQPYHYGSHYSNSGTVLHFLVRLPPFTKMFLAYQDQSFDIPDRTFHSMNTTWRLSSYESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNSERVNHVNLPPWARNDPRLFILIHRQALESDQVSQTLCQWIDLVFGLKQKGKAAVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFSASHISRAGTKLLSEAELPAAMGLLVQLAFRESREQPRDSGYPSPLPWIKGLKWGEYVGSPSAPDPVVCFSQPHGERFGSLLALPTRAICGLSRKFCLMMIYSKEQGVRSMHSTDIQWSAILSWGYTDNMLRLKSKQSEPPINFIQCSPLHQVSSCAWVPDSCQLFTGSKCGVITAYSNRFTTTMPTEMEVESQVDLYGHTAEVTGLFVCKPYSILISVSRDGTCILWDLNRLCYVQSLSGHKSPVNAVSASETTGDIATVCDSVGGGSDLRLWTINGDLIGHVHCREIICSVAFSNQPEGVSVNVIAGGLENGVVRLWSTWDLKPVREITFPKSNRPIISLTFSCDGHHLYTANSEGTVIAWCRRDQQRMKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_009307601.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307601.3

ORF Insert Sequence:

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTCAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
CGTAGACGCT TCACACTGGC CCAGTCGGAC TCTCTGCTGA CCCGCATGCG TTCTGTAGCC 
AGTGATGAGC TGACACAGAT GATGCAGCGC AGGATGAGTC AAGAAAACCC CATCAGAGCC 
AGCGAGACCG AGCTCATCCA GAGAATGCAG AGACTGGTGG TCCTGGCTGT CAACAGACTC 
ATTTATCAAG ACATCAGCCA TGATTTCTTT GACCTGCTGA ACTTCCCTGA GTCTCCTGAT 
CACTCGATGT CCATGCCAAC AGCAGGAGAT CAGACATCTG ATGAGAACGG CTCCACTCCG 
TCTACCCCGA TCCCAGCCGG AAACATCAAG AGCTTCCGGA AGGACATTCT CAGACTGATG 
ATGGAGGGCA TAAAGATCGG CCTGGGCAGC ACTGGTCGTG GCGGCGCTCC TCGTCAGCAG 
TGGCGGCGAA TCCTCTGGTC CTGCCGAGAC ACATTCAGAG TGCAGATCGG CCGCATGCTG 
GTGCACACGC TCAGTCCTGA ACTTCCTCTG GAGGACAGGA AGGAGGCGCT AGAGTTCGTC 
CATGAGCAGA GCTACTCCGA GATCCTCAGA GAAAGCCTCA GCCCCGGCCT CGAGCATGGG 
CCCAAGCTGG CTCTGTATCT GTACGAGCTG CTGCACGATC ACAAAGACGC CCTGAGTAGA 
GAAGAGCAGG CGGCAGGAAA CACCTTCATG ACGGCTCTGA AACTCTGCGG ACACCGCTGC 
ATCCCACCCA GCGCTCCAGC CAAACCCGAC CTCCTGAAAG CCATCAAAGA GGAGCAACTA 
AAGTATGAAT CAGAGGCAAG AACCAGCAAA GGAGCCTGGG AAAAGAAGCT GTCCAACACA 
CAGAGGAGCC TGTTGCAGAG GCTGGATGGC AAGTCGAAGG ACATCTCGAA GATCGCGGCG 
GACATCACTC AGAACGTGTC TCTCAGGCAG GGCATGGAGA GGAAGAAGGT CATGCAGCAC 
ATTAGAGGCC TGTATAAGAC TGACCTGAGC GCTAGTCGAC ACTGGCAGGA GCTCGTCCAG 
CAGCTCACAC ACGACCGGGC GGTGTGGTAC GATCAGTCCT CTTATCCCAC TTCCTGGCAG 
CTGGACCCCA CCGAGGGCCC GAACCGAGAG CGGAGACGCC TCCAGCGCTG CTACCTGACC 
ATCCCCAACA AATATCTGCT CAAAGACCGT CGCAAACTAG ATGATTCCAT TAAGGCTCCT 
CTGGCCTTCC TGTTTGAGGA CAAGACTCAC TCCTCTTCAT CTATAGTGAA GGATAAAGCC 
ACCAGTGAGC CCATCAGGTT CACAAGAAGG TGCGTAAGTG TGGCTCCCTC CAGAGAGACA 
GCAGGAGAGC TCTTACTGGG AAAATCTGGG ATGTATTTTG TGGAGGAAAA TGTTCCTGAG 
ACTCACGATA ACCAGAGTCC TCATGGAGAG ACGGAGCCGG CCTCTTTCTC CTGGACGTAT 
GAGGAGATAA AGGAGGTTCA CAAGCGCTGG TGGCAGCTCA GAGACAACGC CATGGAGATC 
TTCCTGACCA ATGGAAGGAC ACTGCTGCTA GCCTTTGACA ACACAAAGTT TCGTGATGAC 
GTCTACCACA ACATCCTGAC CTCAGATCTG CCCAACCTGC TGGAGTATGG AAACATCAGC 
GCCCTCACGC ACCTCTGGAG CTCAGGACAG ATCACCAACT TTGAGTACCT GACGCATCTC 
AACAAGCACG CGGGACGCTC CTTCAATGAC CTCATGCAGT ATCCCGTCTT CCCCTTCATC 
CTCAAAGATT ACACCAATGA GACGCTCGAC CTGCAGGAGG CCTCTATTTA CAGGAACCTC 
AGCAAGCCCA TTGCAGTGCA GTCTAAAGAG AAGGAAGACC GTTATGTGGA CAATTACAGG 
TATCTGGAGG AGGAGTATAA GAAGGGCAAG AGAGAGGACG ACCCTATGCC CCCTGTCCAG 
CCCTATCACT ACGGCTCTCA TTACTCCAAC AGTGGCACCG TCCTGCACTT CCTGGTCCGA 
CTGCCTCCTT TTACCAAAAT GTTTCTGGCA TATCAAGACC AAAGCTTTGA TATTCCTGAC 
CGAACCTTCC ACTCCATGAA CACCACGTGG CGTCTGTCGT CCTACGAGTC CATGACTGAT 
GTCAAAGAGC TCATCCCAGA GTTCTTCTAC CTGCCCGAGT TCCTGGTCAA CAGAGAAGGT 
TTTGACTTTG GCGTGCGGCA GAACAGCGAG CGGGTCAACC ATGTGAATCT GCCGCCGTGG 
GCCAGAAATG ACCCGCGACT CTTCATCCTC ATCCACAGAC AGGCTCTGGA GTCCGATCAA 
GTCTCGCAGA CGCTCTGCCA GTGGATCGAC CTGGTGTTTG GACTCAAGCA GAAAGGCAAA 
GCTGCGGTCC AGGCCATCAA CGTCTTTCAT CCTGCAACGT ACTTTGGTAT GGACGTGTCT 
GCAGTTGAAG ACCCTGTGCA GCGGCGGGCT CTAGAAACCA TGATCAAGAC GTACGGACAA 
ACACCCAGGC AGCTGTTCAG CGCTTCTCAC ATCAGCAGAG CCGGAACCAA ACTGCTGTCA 
GAGGCTGAGC TGCCCGCCGC CATGGGTCTG CTGGTGCAGC TGGCCTTCAG AGAGAGCCGA 
GAACAGCCCC GGGACAGCGG CTACCCGAGT CCTCTGCCGT GGATAAAGGG CTTGAAATGG 
GGCGAGTATG TGGGTTCACC CAGTGCTCCG GACCCTGTGG TCTGTTTCAG TCAACCTCAC 
GGCGAACGCT TCGGCTCCCT GCTGGCACTG CCCACACGTG CCATCTGCGG CCTCTCACGC 
AAGTTCTGCC TCATGATGAT CTACAGCAAA GAGCAAGGTG TGAGGAGCAT GCACAGCACT 
GACATACAGT GGTCTGCTAT TCTGAGCTGG GGTTACACCG ACAACATGCT GCGTCTGAAG 
AGCAAACAGA GCGAACCACC AATCAACTTC ATCCAGTGCT CACCGCTGCA CCAGGTGAGC 
AGTTGTGCCT GGGTGCCGGA CAGTTGTCAG CTGTTTACAG GCAGCAAGTG TGGAGTCATT 
ACCGCCTACA GCAACCGCTT CACCACCACC ATGCCCACAG AGATGGAGGT GGAGTCGCAG 
GTTGATCTGT ACGGACACAC AGCCGAGGTC ACGGGTCTGT TCGTCTGTAA ACCCTACAGT 
ATCCTCATCA GTGTGAGTCG GGACGGAACC TGCATACTCT GGGATCTCAA CAGGTTGTGT 
TACGTACAGA GTCTCAGCGG TCACAAGAGT CCAGTGAACG CGGTGTCAGC CAGCGAGACC 
ACCGGAGACA TCGCCACCGT CTGCGACTCA GTGGGTGGAG GAAGTGACCT GCGGCTGTGG 
ACCATTAATG GAGATCTGAT TGGTCACGTT CACTGTCGTG AGATCATCTG TTCAGTGGCT 
TTCTCCAATC AGCCGGAGGG AGTTTCAGTC AATGTGATCG CAGGCGGACT GGAGAACGGG 
GTGGTCAGGT TGTGGAGCAC GTGGGACCTG AAGCCAGTCA GAGAGATCAC GTTCCCAAAG 
TCCAACAGAC CTATCATAAG TCTGACGTTC TCATGTGACG GTCATCATCT ATACACGGCT 
AACAGTGAGG GCACGGTGAT CGCGTGGTGT CGGCGAGACC AGCAGCGCAT GAAGCTGCCC 
ATGTTTTACT CTTTCCTCAG CAGTTACGCC GCAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lyst ( XM_017358861.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214350.1 Danio rerio lysosomal trafficking regulator (lyst), transcript variant X1, mRNA.

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CloneID	ODa54787
Clone ID Related Accession (Same CDS sequence)	XM_017358861.1 , XM_017358861.2
Accession Version	XM_017358861.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	lysosomal-trafficking regulator isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTCAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
TCAATGGGGC TGAAAGACTC CATAATCCCC CGTATCCCCC GGCAGCACTA CTGCTCGTAC 
GGCCTGATCC CTCTGCCCTC TCCGTTCAGC TTCCTGTCCC AGGATTCCCC TCTCCCTTCC 
CCTACCGGGT CAAACTCCTA CACGTTCCCC TCAGACGCAG GTCGTAGACG CTTCACACTG 
GCCCAGTCGG ACTCTCTGCT GACCCGCATG CGTTCTGTAG CCAGTGATGA GCTGACACAG 
ATGATGCAGC GCAGGATGAG TCAAGAAAAC CCCATCAGAG CCAGCGAGAC CGAGCTCATC 
CAGAGAATGC AGAGACTGGT GGTCCTGGCT GTCAACAGAC TCATTTATCA AGACATCAGC 
CATGATTTCT TTGACCTGCT GAACTTCCCT GAGTCTCCTG ATCACTCGAT GTCCATGCCA 
ACAGCAGGAG ATCAGACATC TGATGAGAAC GGCTCCACTC CGTCTACCCC GATCCCAGCC 
GGAAACATCA AGAGCTTCCG GAAGGACATT CTCAGACTGA TGATGGAGGG CATAAAGATC 
GGCCTGGGCA GCACTGGTCG TGGCGGCGCT CCTCGTCAGC AGTGGCGGCG AATCCTCTGG 
TCCTGCCGAG ACACATTCAG AGTGCAGATC GGCCGCATGC TGGTGCACAC GCTCAGTCCT 
GAACTTCCTC TGGAGGACAG GAAGGAGGCG CTAGAGTTCG TCCATGAGCA GAGCTACTCC 
GAGATCCTCA GAGAAAGCCT CAGCCCCGGC CTCGAGCATG GGCCCAAGCT GGCTCTGTAT 
CTGTACGAGC TGCTGCACGA TCACAAAGAC GCCCTGAGTA GAGAAGAGCA GGCGGCAGGA 
AACACCTTCA TGACGGCTCT GAAACTCTGC GGACACCGCT GCATCCCACC CAGCGCTCCA 
GCCAAACCCG ACCTCCTGAA AGCCATCAAA GAGGAGCAAC TAAAGTATGA ATCAGAGGCA 
AGAACCAGCA AAGGAGCCTG GGAAAAGAAG CTGTCCAACA CACAGAGGAG CCTGTTGCAG 
AGGCTGGATG GCAAGTCGAA GGACATCTCG AAGATCGCGG CGGACATCAC TCAGAACGTG 
TCTCTCAGGC AGGGCATGGA GAGGAAGAAG GTCATGCAGC ACATTAGAGG CCTGTATAAG 
ACTGACCTGA GCGCTAGTCG ACACTGGCAG GAGCTCGTCC AGCAGCTCAC ACACGACCGG 
GCGGTGTGGT ACGATCAGTC CTCTTATCCC ACTTCCTGGC AGCTGGACCC CACCGAGGGC 
CCGAACCGAG AGCGGAGACG CCTCCAGCGC TGCTACCTGA CCATCCCCAA CAAATATCTG 
CTCAAAGACC GTCGCAAACT AGATGATTCC ATTAAGGCTC CTCTGGCCTT CCTGTTTGAG 
GACAAGACTC ACTCCTCTTC ATCTATAGTG AAGGATAAAG CCACCAGTGA GCCCATCAGG 
TTCACAAGAA GGTGCGTAAG TGTGGCTCCC TCCAGAGAGA CAGCAGGAGA GCTCTTACTG 
GGAAAATCTG GGATGTATTT TGTGGAGGAA AATGTTCCTG AGACTCACGA TAACCAGAGT 
CCTCATGGAG AGACGGAGCC GGCCTCTTTC TCCTGGACGT ATGAGGAGAT AAAGGAGGTT 
CACAAGCGCT GGTGGCAGCT CAGAGACAAC GCCATGGAGA TCTTCCTGAC CAATGGAAGG 
ACACTGCTGC TAGCCTTTGA CAACACAAAG TTTCGTGATG ACGTCTACCA CAACATCCTG 
ACCTCAGATC TGCCCAACCT GCTGGAGTAT GGAAACATCA GCGCCCTCAC GCACCTCTGG 
AGCTCAGGAC AGATCACCAA CTTTGAGTAC CTGACGCATC TCAACAAGCA CGCGGGACGC 
TCCTTCAATG ACCTCATGCA GTATCCCGTC TTCCCCTTCA TCCTCAAAGA TTACACCAAT 
GAGACGCTCG ACCTGCAGGA GGCCTCTATT TACAGGAACC TCAGCAAGCC CATTGCAGTG 
CAGTCTAAAG AGAAGGAAGA CCGTTATGTG GACAATTACA GGTATCTGGA GGAGGAGTAT 
AAGAAGGGCA AGAGAGAGGA CGACCCTATG CCCCCTGTCC AGCCCTATCA CTACGGCTCT 
CATTACTCCA ACAGTGGCAC CGTCCTGCAC TTCCTGGTCC GACTGCCTCC TTTTACCAAA 
ATGTTTCTGG CATATCAAGA CCAAAGCTTT GATATTCCTG ACCGAACCTT CCACTCCATG 
AACACCACGT GGCGTCTGTC GTCCTACGAG TCCATGACTG ATGTCAAAGA GCTCATCCCA 
GAGTTCTTCT ACCTGCCCGA GTTCCTGGTC AACAGAGAAG GTTTTGACTT TGGCGTGCGG 
CAGAACAGCG AGCGGGTCAA CCATGTGAAT CTGCCGCCGT GGGCCAGAAA TGACCCGCGA 
CTCTTCATCC TCATCCACAG ACAGGCTCTG GAGTCCGATC AAGTCTCGCA GACGCTCTGC 
CAGTGGATCG ACCTGGTGTT TGGACTCAAG CAGAAAGGCA AAGCTGCGGT CCAGGCCATC 
AACGTCTTTC ATCCTGCAAC GTACTTTGGT ATGGACGTGT CTGCAGTTGA AGACCCTGTG 
CAGCGGCGGG CTCTAGAAAC CATGATCAAG ACGTACGGAC AAACACCCAG GCAGCTGTTC 
AGCGCTTCTC ACATCAGCAG AGCCGGAACC AAACTGCTGT CAGAGGCTGA GCTGCCCGCC 
GCCATGGGTC TGCTGGTGCA GCTGGCCTTC AGAGAGAGCC GAGAACAGCC CCGGGACAGC 
GGCTACCCGA GTCCTCTGCC GTGGATAAAG GGCTTGAAAT GGGGCGAGTA TGTGGGTTCA 
CCCAGTGCTC CGGACCCTGT GGTCTGTTTC AGTCAACCTC ACGGCGAACG CTTCGGCTCC 
CTGCTGGCAC TGCCCACACG TGCCATCTGC GGCCTCTCAC GCAAGTTCTG CCTCATGATG 
ATCTACAGCA AAGAGCAAGG TGTGAGGAGC ATGCACAGCA CTGACATACA GTGGTCTGCT 
ATTCTGAGCT GGGGTTACAC CGACAACATG CTGCGTCTGA AGAGCAAACA GAGCGAACCA 
CCAATCAACT TCATCCAGTG CTCACCGCTG CACCAGGTGA GCAGTTGTGC CTGGGTGCCG 
GACAGTTGTC AGCTGTTTAC AGGCAGCAAG TGTGGAGTCA TTACCGCCTA CAGCAACCGC 
TTCACCACCA CCATGCCCAC AGAGATGGAG GTGGAGTCGC AGGTTGATCT GTACGGACAC 
ACAGCCGAGG TCACGGGTCT GTTCGTCTGT AAACCCTACA GTATCCTCAT CAGTGTGAGT 
CGGGACGGAA CCTGCATACT CTGGGATCTC AACAGGTTGT GTTACGTACA GAGTCTCAGC 
GGTCACAAGA GTCCAGTGAA CGCGGTGTCA GCCAGCGAGA CCACCGGAGA CATCGCCACC 
GTCTGCGACT CAGTGGGTGG AGGAAGTGAC CTGCGGCTGT GGACCATTAA TGGAGATCTG 
ATTGGTCACG TTCACTGTCG TGAGATCATC TGTTCAGTGG CTTTCTCCAA TCAGCCGGAG 
GGAGTTTCAG TCAATGTGAT CGCAGGCGGA CTGGAGAACG GGGTGGTCAG GTTGTGGAGC 
ACGTGGGACC TGAAGCCAGT CAGAGAGATC ACGTTCCCAA AGTCCAACAG ACCTATCATA 
AGTCTGACGT TCTCATGTGA CGGTCATCAT CTATACACGG CTAACAGTGA GGGCACGGTG 
ATCGCGTGGT GTCGGCGAGA CCAGCAGCGC ATGAAGCTGC CCATGTTTTA CTCTTTCCTC 
AGCAGTTACG CCGCAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214350.1
CDS	198..11858
Translation	MASSHLADLSQAWENYMDCRTQGADLQVSLQCLESFLCTFHCVRHLSDPRAKDALKFCSDMSGASNTLAREFLTDVHQLCSAVAQRAEDEEESHMVALGEYLVRGRGFLLLSTLESVIDQELTCREELLTLLLSLLPLVWKIPVQEEKAPDFTLPSLLEVFLCQEGRTISQKAGQDPCDVQNSTKRLSGSWKSRRSRRTAQRYSVRDARKSQLSTSDSEANSDDKTTNTGSKHRRFHSTAIHVSCQLHQSNNSSQPAPQHPTLDAMGTFEPSQPHLRLFGSHTMDPNMLSDPTTLSIFNRMENSPFDLCHVLLSLLEKVCKFDMTINHNPGLAVSVVPTLTEILTDFGDCCGPSGGGGGGIDELAGGWTEEPIALVQRMLLRTILHLMFVDVGQNETLPDNLRRSLTDLLRATLKIRTCLERQADPFAPRPKKTLQEIQEDFSFSRYRHRALLLPELLQGVLQLLLGCLQASAPNPFFFSQSLELVHEFVQHRGLELFEVTALRLEALAEAHNSEISNEASEHLRCLIADVLKIISAVKRAKSEQLHQSVCARRRHRRCEYSHFLHHHRDLSGLPVSAFKQAARRNPFEEDSKGNDGEVRYPERCCCLAACAHQCLRLFQRLSPSGPAVLQVLAGVQAVGICCCMDPRSVIAPMLQAFQAPGLRSYQSHVLSVLSRLVLEQLGGGQPSEKAKLASCNICTLDSSQLPGLEETLQGCGPKKELNLSTSPSPSYRSQGILPSGGAEDILWKWDALEAYQELVFGEDWHLSQQIASHVCQLTLRGNAVVQWQLYTHIFNPVLQRGVELTHHAQQLGVSTTCSQVCSYHTHCLPVEVLLVYLQTLPALLKSSRVVRDLFLSCNGLNQVTELIYLDCTRPWALKVFETLILNAWRNHTESAVHELERAEAQEREAVLGLVEELSTRGTGDAPQSLTKFYEGLKEACPPPKGKSETSEFSGVGRNRGEAHLNTINLFLCVAFLCVSKEADSDRDSANDSEDTSGYDSTASEPLGGRLPCLSPDSVALPSKEQVRRAADVWSVCRWVYMASPVFQRQFFRLGGLDVCSRLMTMVIQKLTSKNKDCKFKKKRESKGKSSPSREDSPTRCITGHFDNMVTNAGTAETQHPELSDTVSLSQTESKSSDPMHRLEEEWPFQSIRLLEALLAICLHSANSALQRLEPDLSFQLQSVEETLCEVRDQLSRSGVVNSELAVPLFDSLLRVALAEMFCSPEVIEEKPDKKCAVSEATPPAGDLSEDVDEAHVSAVQPLGEEEGYDADSESNPEDSTRQEEGLKVEAGVSLRECTLGMAGVPQGSVRGRLLFPEICLMELQLLASGSPDLEVLGHVLQSLLAAVKGHHNNAAQLYQQGGVKVILTGFPNILSQSDASYKECQTVLMELLVAMVSDRITAEELALLTRLFLEKTPPTEILLRGVLQIVEANTDTEPLYFMSFPMIPGSGGPGLSPSPGTRPHGATAGKGGVNTILRGKLPLGRGEADMSRPGHLRSSPWHAAPFHLPLVGQNCWPHMASGFSASLWVRAGKDEDGRSKKEDEVCVAEESPHGSAHVTEQQTERCLLHVLSMGSKALMLQVWVNISTRAFTFRICIDPNDEMKAGLLAQAESGDEVIPAGRWQHLALTYTQQPEGKKNIHGQLSLWVCGIRKCDVSLDFTLPRKSSLSSDSNKTYCMLGHYQHSAEEQASPSARWDMGTLMLFNGSRIDTTEAFYLYASGPDLTSIMPCKYGKPHGTLSKYVTQEGLQCEQIRELLMRNADVDISPLVESLAVVYTPSTPSLYTIYEPVIRLKGQAKSAVVSQRPFSAKEVQSSILESPFLRTMMPCQAFGLHSALHKIGGTGSVVFLFARAVELCDCEKTQALALQVLLSLVKNNQHRTHEMECYHGYSMIHQVLIKPKCIVGYHILKTLLDGCCSGSILTIGEDGQFHLDAESTVVVQDLRLLSEILLDWKIWAKAQCGVWETLLTALEILIRVRHPQQMFNIRQLLKAQVVHRFLLACQVLQEHRDEYLTSIPQEVCLSFVKIIQEVLGSPPDMDLLRLVYNFLLAVHPPTNTYVCHTPTSFYFSLHIDGKLYQEKVQSMMYLRHSSSGGKSACSSVVSLSPTVFTEMPAEVRHTSSIEHEDDLSLRPRSTGPSPHSSPALTPRLTHGRVTGTTEPEGGASLSTQQALGSTETLKRAGDEQLLSSCESAKTICDSPIAGADEEGSIMGNRTPSISVEEEQDGPAGSPVESEEHFDWTSEEAPRRPDSLKGIQSFQRSHSNLASLGLAFPAQNGSLTIARWPTVADRAAPPDDWESYTYSPGYDRHSKADSSNDRSSAEDCLVLICCGLYDLLRGVLLLLPDLLLEEVMDKLIQPDALLVLVNHPSPLIQQGVMKLLDAYFSRAQKEQKEKFLKNHGFSLLANQLYQHQGSQGLLECFLEMLFGRPVGLEEDLDLEDMENISPFRKRCIIPVLGLLENSLYENSLVHNGLCLLLQLLNACPKLADILLDHGLLYVLFNTLSTLNGLENGIPPNDYRLVVCDIQQLLVAVTIHSCSSSGSQYFRIIEDLITLLGYMQTSKVRRTQEMALALQFRVIQAAIDFIKTTANQDPQKSGSYVHVPTSPHHALQQKRKSIAGSMGLKDSIIPRIPRQHYCSYGLIPLPSPFSFLSQDSPLPSPTGSNSYTFPSDAGRRRFTLAQSDSLLTRMRSVASDELTQMMQRRMSQENPIRASETELIQRMQRLVVLAVNRLIYQDISHDFFDLLNFPESPDHSMSMPTAGDQTSDENGSTPSTPIPAGNIKSFRKDILRLMMEGIKIGLGSTGRGGAPRQQWRRILWSCRDTFRVQIGRMLVHTLSPELPLEDRKEALEFVHEQSYSEILRESLSPGLEHGPKLALYLYELLHDHKDALSREEQAAGNTFMTALKLCGHRCIPPSAPAKPDLLKAIKEEQLKYESEARTSKGAWEKKLSNTQRSLLQRLDGKSKDISKIAADITQNVSLRQGMERKKVMQHIRGLYKTDLSASRHWQELVQQLTHDRAVWYDQSSYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLKDRRKLDDSIKAPLAFLFEDKTHSSSSIVKDKATSEPIRFTRRCVSVAPSRETAGELLLGKSGMYFVEENVPETHDNQSPHGETEPASFSWTYEEIKEVHKRWWQLRDNAMEIFLTNGRTLLLAFDNTKFRDDVYHNILTSDLPNLLEYGNISALTHLWSSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILKDYTNETLDLQEASIYRNLSKPIAVQSKEKEDRYVDNYRYLEEEYKKGKREDDPMPPVQPYHYGSHYSNSGTVLHFLVRLPPFTKMFLAYQDQSFDIPDRTFHSMNTTWRLSSYESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNSERVNHVNLPPWARNDPRLFILIHRQALESDQVSQTLCQWIDLVFGLKQKGKAAVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFSASHISRAGTKLLSEAELPAAMGLLVQLAFRESREQPRDSGYPSPLPWIKGLKWGEYVGSPSAPDPVVCFSQPHGERFGSLLALPTRAICGLSRKFCLMMIYSKEQGVRSMHSTDIQWSAILSWGYTDNMLRLKSKQSEPPINFIQCSPLHQVSSCAWVPDSCQLFTGSKCGVITAYSNRFTTTMPTEMEVESQVDLYGHTAEVTGLFVCKPYSILISVSRDGTCILWDLNRLCYVQSLSGHKSPVNAVSASETTGDIATVCDSVGGGSDLRLWTINGDLIGHVHCREIICSVAFSNQPEGVSVNVIAGGLENGVVRLWSTWDLKPVREITFPKSNRPIISLTFSCDGHHLYTANSEGTVIAWCRRDQQRMKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_017358861.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358861.1

ORF Insert Sequence:

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTCAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
TCAATGGGGC TGAAAGACTC CATAATCCCC CGTATCCCCC GGCAGCACTA CTGCTCGTAC 
GGCCTGATCC CTCTGCCCTC TCCGTTCAGC TTCCTGTCCC AGGATTCCCC TCTCCCTTCC 
CCTACCGGGT CAAACTCCTA CACGTTCCCC TCAGACGCAG GTCGTAGACG CTTCACACTG 
GCCCAGTCGG ACTCTCTGCT GACCCGCATG CGTTCTGTAG CCAGTGATGA GCTGACACAG 
ATGATGCAGC GCAGGATGAG TCAAGAAAAC CCCATCAGAG CCAGCGAGAC CGAGCTCATC 
CAGAGAATGC AGAGACTGGT GGTCCTGGCT GTCAACAGAC TCATTTATCA AGACATCAGC 
CATGATTTCT TTGACCTGCT GAACTTCCCT GAGTCTCCTG ATCACTCGAT GTCCATGCCA 
ACAGCAGGAG ATCAGACATC TGATGAGAAC GGCTCCACTC CGTCTACCCC GATCCCAGCC 
GGAAACATCA AGAGCTTCCG GAAGGACATT CTCAGACTGA TGATGGAGGG CATAAAGATC 
GGCCTGGGCA GCACTGGTCG TGGCGGCGCT CCTCGTCAGC AGTGGCGGCG AATCCTCTGG 
TCCTGCCGAG ACACATTCAG AGTGCAGATC GGCCGCATGC TGGTGCACAC GCTCAGTCCT 
GAACTTCCTC TGGAGGACAG GAAGGAGGCG CTAGAGTTCG TCCATGAGCA GAGCTACTCC 
GAGATCCTCA GAGAAAGCCT CAGCCCCGGC CTCGAGCATG GGCCCAAGCT GGCTCTGTAT 
CTGTACGAGC TGCTGCACGA TCACAAAGAC GCCCTGAGTA GAGAAGAGCA GGCGGCAGGA 
AACACCTTCA TGACGGCTCT GAAACTCTGC GGACACCGCT GCATCCCACC CAGCGCTCCA 
GCCAAACCCG ACCTCCTGAA AGCCATCAAA GAGGAGCAAC TAAAGTATGA ATCAGAGGCA 
AGAACCAGCA AAGGAGCCTG GGAAAAGAAG CTGTCCAACA CACAGAGGAG CCTGTTGCAG 
AGGCTGGATG GCAAGTCGAA GGACATCTCG AAGATCGCGG CGGACATCAC TCAGAACGTG 
TCTCTCAGGC AGGGCATGGA GAGGAAGAAG GTCATGCAGC ACATTAGAGG CCTGTATAAG 
ACTGACCTGA GCGCTAGTCG ACACTGGCAG GAGCTCGTCC AGCAGCTCAC ACACGACCGG 
GCGGTGTGGT ACGATCAGTC CTCTTATCCC ACTTCCTGGC AGCTGGACCC CACCGAGGGC 
CCGAACCGAG AGCGGAGACG CCTCCAGCGC TGCTACCTGA CCATCCCCAA CAAATATCTG 
CTCAAAGACC GTCGCAAACT AGATGATTCC ATTAAGGCTC CTCTGGCCTT CCTGTTTGAG 
GACAAGACTC ACTCCTCTTC ATCTATAGTG AAGGATAAAG CCACCAGTGA GCCCATCAGG 
TTCACAAGAA GGTGCGTAAG TGTGGCTCCC TCCAGAGAGA CAGCAGGAGA GCTCTTACTG 
GGAAAATCTG GGATGTATTT TGTGGAGGAA AATGTTCCTG AGACTCACGA TAACCAGAGT 
CCTCATGGAG AGACGGAGCC GGCCTCTTTC TCCTGGACGT ATGAGGAGAT AAAGGAGGTT 
CACAAGCGCT GGTGGCAGCT CAGAGACAAC GCCATGGAGA TCTTCCTGAC CAATGGAAGG 
ACACTGCTGC TAGCCTTTGA CAACACAAAG TTTCGTGATG ACGTCTACCA CAACATCCTG 
ACCTCAGATC TGCCCAACCT GCTGGAGTAT GGAAACATCA GCGCCCTCAC GCACCTCTGG 
AGCTCAGGAC AGATCACCAA CTTTGAGTAC CTGACGCATC TCAACAAGCA CGCGGGACGC 
TCCTTCAATG ACCTCATGCA GTATCCCGTC TTCCCCTTCA TCCTCAAAGA TTACACCAAT 
GAGACGCTCG ACCTGCAGGA GGCCTCTATT TACAGGAACC TCAGCAAGCC CATTGCAGTG 
CAGTCTAAAG AGAAGGAAGA CCGTTATGTG GACAATTACA GGTATCTGGA GGAGGAGTAT 
AAGAAGGGCA AGAGAGAGGA CGACCCTATG CCCCCTGTCC AGCCCTATCA CTACGGCTCT 
CATTACTCCA ACAGTGGCAC CGTCCTGCAC TTCCTGGTCC GACTGCCTCC TTTTACCAAA 
ATGTTTCTGG CATATCAAGA CCAAAGCTTT GATATTCCTG ACCGAACCTT CCACTCCATG 
AACACCACGT GGCGTCTGTC GTCCTACGAG TCCATGACTG ATGTCAAAGA GCTCATCCCA 
GAGTTCTTCT ACCTGCCCGA GTTCCTGGTC AACAGAGAAG GTTTTGACTT TGGCGTGCGG 
CAGAACAGCG AGCGGGTCAA CCATGTGAAT CTGCCGCCGT GGGCCAGAAA TGACCCGCGA 
CTCTTCATCC TCATCCACAG ACAGGCTCTG GAGTCCGATC AAGTCTCGCA GACGCTCTGC 
CAGTGGATCG ACCTGGTGTT TGGACTCAAG CAGAAAGGCA AAGCTGCGGT CCAGGCCATC 
AACGTCTTTC ATCCTGCAAC GTACTTTGGT ATGGACGTGT CTGCAGTTGA AGACCCTGTG 
CAGCGGCGGG CTCTAGAAAC CATGATCAAG ACGTACGGAC AAACACCCAG GCAGCTGTTC 
AGCGCTTCTC ACATCAGCAG AGCCGGAACC AAACTGCTGT CAGAGGCTGA GCTGCCCGCC 
GCCATGGGTC TGCTGGTGCA GCTGGCCTTC AGAGAGAGCC GAGAACAGCC CCGGGACAGC 
GGCTACCCGA GTCCTCTGCC GTGGATAAAG GGCTTGAAAT GGGGCGAGTA TGTGGGTTCA 
CCCAGTGCTC CGGACCCTGT GGTCTGTTTC AGTCAACCTC ACGGCGAACG CTTCGGCTCC 
CTGCTGGCAC TGCCCACACG TGCCATCTGC GGCCTCTCAC GCAAGTTCTG CCTCATGATG 
ATCTACAGCA AAGAGCAAGG TGTGAGGAGC ATGCACAGCA CTGACATACA GTGGTCTGCT 
ATTCTGAGCT GGGGTTACAC CGACAACATG CTGCGTCTGA AGAGCAAACA GAGCGAACCA 
CCAATCAACT TCATCCAGTG CTCACCGCTG CACCAGGTGA GCAGTTGTGC CTGGGTGCCG 
GACAGTTGTC AGCTGTTTAC AGGCAGCAAG TGTGGAGTCA TTACCGCCTA CAGCAACCGC 
TTCACCACCA CCATGCCCAC AGAGATGGAG GTGGAGTCGC AGGTTGATCT GTACGGACAC 
ACAGCCGAGG TCACGGGTCT GTTCGTCTGT AAACCCTACA GTATCCTCAT CAGTGTGAGT 
CGGGACGGAA CCTGCATACT CTGGGATCTC AACAGGTTGT GTTACGTACA GAGTCTCAGC 
GGTCACAAGA GTCCAGTGAA CGCGGTGTCA GCCAGCGAGA CCACCGGAGA CATCGCCACC 
GTCTGCGACT CAGTGGGTGG AGGAAGTGAC CTGCGGCTGT GGACCATTAA TGGAGATCTG 
ATTGGTCACG TTCACTGTCG TGAGATCATC TGTTCAGTGG CTTTCTCCAA TCAGCCGGAG 
GGAGTTTCAG TCAATGTGAT CGCAGGCGGA CTGGAGAACG GGGTGGTCAG GTTGTGGAGC 
ACGTGGGACC TGAAGCCAGT CAGAGAGATC ACGTTCCCAA AGTCCAACAG ACCTATCATA 
AGTCTGACGT TCTCATGTGA CGGTCATCAT CTATACACGG CTAACAGTGA GGGCACGGTG 
ATCGCGTGGT GTCGGCGAGA CCAGCAGCGC ATGAAGCTGC CCATGTTTTA CTCTTTCCTC 
AGCAGTTACG CCGCAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lyst ( XM_017358861.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214350.1 Danio rerio lysosomal trafficking regulator (lyst), transcript variant X1, mRNA.

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CloneID	ODa54787
Clone ID Related Accession (Same CDS sequence)	XM_017358861.1 , XM_017358861.2
Accession Version	XM_017358861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lysosomal-trafficking regulator isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTCAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
TCAATGGGGC TGAAAGACTC CATAATCCCC CGTATCCCCC GGCAGCACTA CTGCTCGTAC 
GGCCTGATCC CTCTGCCCTC TCCGTTCAGC TTCCTGTCCC AGGATTCCCC TCTCCCTTCC 
CCTACCGGGT CAAACTCCTA CACGTTCCCC TCAGACGCAG GTCGTAGACG CTTCACACTG 
GCCCAGTCGG ACTCTCTGCT GACCCGCATG CGTTCTGTAG CCAGTGATGA GCTGACACAG 
ATGATGCAGC GCAGGATGAG TCAAGAAAAC CCCATCAGAG CCAGCGAGAC CGAGCTCATC 
CAGAGAATGC AGAGACTGGT GGTCCTGGCT GTCAACAGAC TCATTTATCA AGACATCAGC 
CATGATTTCT TTGACCTGCT GAACTTCCCT GAGTCTCCTG ATCACTCGAT GTCCATGCCA 
ACAGCAGGAG ATCAGACATC TGATGAGAAC GGCTCCACTC CGTCTACCCC GATCCCAGCC 
GGAAACATCA AGAGCTTCCG GAAGGACATT CTCAGACTGA TGATGGAGGG CATAAAGATC 
GGCCTGGGCA GCACTGGTCG TGGCGGCGCT CCTCGTCAGC AGTGGCGGCG AATCCTCTGG 
TCCTGCCGAG ACACATTCAG AGTGCAGATC GGCCGCATGC TGGTGCACAC GCTCAGTCCT 
GAACTTCCTC TGGAGGACAG GAAGGAGGCG CTAGAGTTCG TCCATGAGCA GAGCTACTCC 
GAGATCCTCA GAGAAAGCCT CAGCCCCGGC CTCGAGCATG GGCCCAAGCT GGCTCTGTAT 
CTGTACGAGC TGCTGCACGA TCACAAAGAC GCCCTGAGTA GAGAAGAGCA GGCGGCAGGA 
AACACCTTCA TGACGGCTCT GAAACTCTGC GGACACCGCT GCATCCCACC CAGCGCTCCA 
GCCAAACCCG ACCTCCTGAA AGCCATCAAA GAGGAGCAAC TAAAGTATGA ATCAGAGGCA 
AGAACCAGCA AAGGAGCCTG GGAAAAGAAG CTGTCCAACA CACAGAGGAG CCTGTTGCAG 
AGGCTGGATG GCAAGTCGAA GGACATCTCG AAGATCGCGG CGGACATCAC TCAGAACGTG 
TCTCTCAGGC AGGGCATGGA GAGGAAGAAG GTCATGCAGC ACATTAGAGG CCTGTATAAG 
ACTGACCTGA GCGCTAGTCG ACACTGGCAG GAGCTCGTCC AGCAGCTCAC ACACGACCGG 
GCGGTGTGGT ACGATCAGTC CTCTTATCCC ACTTCCTGGC AGCTGGACCC CACCGAGGGC 
CCGAACCGAG AGCGGAGACG CCTCCAGCGC TGCTACCTGA CCATCCCCAA CAAATATCTG 
CTCAAAGACC GTCGCAAACT AGATGATTCC ATTAAGGCTC CTCTGGCCTT CCTGTTTGAG 
GACAAGACTC ACTCCTCTTC ATCTATAGTG AAGGATAAAG CCACCAGTGA GCCCATCAGG 
TTCACAAGAA GGTGCGTAAG TGTGGCTCCC TCCAGAGAGA CAGCAGGAGA GCTCTTACTG 
GGAAAATCTG GGATGTATTT TGTGGAGGAA AATGTTCCTG AGACTCACGA TAACCAGAGT 
CCTCATGGAG AGACGGAGCC GGCCTCTTTC TCCTGGACGT ATGAGGAGAT AAAGGAGGTT 
CACAAGCGCT GGTGGCAGCT CAGAGACAAC GCCATGGAGA TCTTCCTGAC CAATGGAAGG 
ACACTGCTGC TAGCCTTTGA CAACACAAAG TTTCGTGATG ACGTCTACCA CAACATCCTG 
ACCTCAGATC TGCCCAACCT GCTGGAGTAT GGAAACATCA GCGCCCTCAC GCACCTCTGG 
AGCTCAGGAC AGATCACCAA CTTTGAGTAC CTGACGCATC TCAACAAGCA CGCGGGACGC 
TCCTTCAATG ACCTCATGCA GTATCCCGTC TTCCCCTTCA TCCTCAAAGA TTACACCAAT 
GAGACGCTCG ACCTGCAGGA GGCCTCTATT TACAGGAACC TCAGCAAGCC CATTGCAGTG 
CAGTCTAAAG AGAAGGAAGA CCGTTATGTG GACAATTACA GGTATCTGGA GGAGGAGTAT 
AAGAAGGGCA AGAGAGAGGA CGACCCTATG CCCCCTGTCC AGCCCTATCA CTACGGCTCT 
CATTACTCCA ACAGTGGCAC CGTCCTGCAC TTCCTGGTCC GACTGCCTCC TTTTACCAAA 
ATGTTTCTGG CATATCAAGA CCAAAGCTTT GATATTCCTG ACCGAACCTT CCACTCCATG 
AACACCACGT GGCGTCTGTC GTCCTACGAG TCCATGACTG ATGTCAAAGA GCTCATCCCA 
GAGTTCTTCT ACCTGCCCGA GTTCCTGGTC AACAGAGAAG GTTTTGACTT TGGCGTGCGG 
CAGAACAGCG AGCGGGTCAA CCATGTGAAT CTGCCGCCGT GGGCCAGAAA TGACCCGCGA 
CTCTTCATCC TCATCCACAG ACAGGCTCTG GAGTCCGATC AAGTCTCGCA GACGCTCTGC 
CAGTGGATCG ACCTGGTGTT TGGACTCAAG CAGAAAGGCA AAGCTGCGGT CCAGGCCATC 
AACGTCTTTC ATCCTGCAAC GTACTTTGGT ATGGACGTGT CTGCAGTTGA AGACCCTGTG 
CAGCGGCGGG CTCTAGAAAC CATGATCAAG ACGTACGGAC AAACACCCAG GCAGCTGTTC 
AGCGCTTCTC ACATCAGCAG AGCCGGAACC AAACTGCTGT CAGAGGCTGA GCTGCCCGCC 
GCCATGGGTC TGCTGGTGCA GCTGGCCTTC AGAGAGAGCC GAGAACAGCC CCGGGACAGC 
GGCTACCCGA GTCCTCTGCC GTGGATAAAG GGCTTGAAAT GGGGCGAGTA TGTGGGTTCA 
CCCAGTGCTC CGGACCCTGT GGTCTGTTTC AGTCAACCTC ACGGCGAACG CTTCGGCTCC 
CTGCTGGCAC TGCCCACACG TGCCATCTGC GGCCTCTCAC GCAAGTTCTG CCTCATGATG 
ATCTACAGCA AAGAGCAAGG TGTGAGGAGC ATGCACAGCA CTGACATACA GTGGTCTGCT 
ATTCTGAGCT GGGGTTACAC CGACAACATG CTGCGTCTGA AGAGCAAACA GAGCGAACCA 
CCAATCAACT TCATCCAGTG CTCACCGCTG CACCAGGTGA GCAGTTGTGC CTGGGTGCCG 
GACAGTTGTC AGCTGTTTAC AGGCAGCAAG TGTGGAGTCA TTACCGCCTA CAGCAACCGC 
TTCACCACCA CCATGCCCAC AGAGATGGAG GTGGAGTCGC AGGTTGATCT GTACGGACAC 
ACAGCCGAGG TCACGGGTCT GTTCGTCTGT AAACCCTACA GTATCCTCAT CAGTGTGAGT 
CGGGACGGAA CCTGCATACT CTGGGATCTC AACAGGTTGT GTTACGTACA GAGTCTCAGC 
GGTCACAAGA GTCCAGTGAA CGCGGTGTCA GCCAGCGAGA CCACCGGAGA CATCGCCACC 
GTCTGCGACT CAGTGGGTGG AGGAAGTGAC CTGCGGCTGT GGACCATTAA TGGAGATCTG 
ATTGGTCACG TTCACTGTCG TGAGATCATC TGTTCAGTGG CTTTCTCCAA TCAGCCGGAG 
GGAGTTTCAG TCAATGTGAT CGCAGGCGGA CTGGAGAACG GGGTGGTCAG GTTGTGGAGC 
ACGTGGGACC TGAAGCCAGT CAGAGAGATC ACGTTCCCAA AGTCCAACAG ACCTATCATA 
AGTCTGACGT TCTCATGTGA CGGTCATCAT CTATACACGG CTAACAGTGA GGGCACGGTG 
ATCGCGTGGT GTCGGCGAGA CCAGCAGCGC ATGAAGCTGC CCATGTTTTA CTCTTTCCTC 
AGCAGTTACG CCGCAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214350.1
CDS	202..11862
Translation	MASSHLADLSQAWENYMDCRTQGADLQVSLQCLESFLCTFHCVRHLSDPRAKDALKFCSDMSGASNTLAREFLTDVHQLCSAVAQRAEDEEESHMVALGEYLVRGRGFLLLSTLESVIDQELTCREELLTLLLSLLPLVWKIPVQEEKAPDFTLPSLLEVFLCQEGRTISQKAGQDPCDVQNSTKRLSGSWKSRRSRRTAQRYSVRDARKSQLSTSDSEANSDDKTTNTGSKHRRFHSTAIHVSCQLHQSNNSSQPAPQHPTLDAMGTFEPSQPHLRLFGSHTMDPNMLSDPTTLSIFNRMENSPFDLCHVLLSLLEKVCKFDMTINHNPGLAVSVVPTLTEILTDFGDCCGPSGGGGGGIDELAGGWTEEPIALVQRMLLRTILHLMFVDVGQNETLPDNLRRSLTDLLRATLKIRTCLERQADPFAPRPKKTLQEIQEDFSFSRYRHRALLLPELLQGVLQLLLGCLQASAPNPFFFSQSLELVHEFVQHRGLELFEVTALRLEALAEAHNSEISNEASEHLRCLIADVLKIISAVKRAKSEQLHQSVCARRRHRRCEYSHFLHHHRDLSGLPVSAFKQAARRNPFEEDSKGNDGEVRYPERCCCLAACAHQCLRLFQRLSPSGPAVLQVLAGVQAVGICCCMDPRSVIAPMLQAFQAPGLRSYQSHVLSVLSRLVLEQLGGGQPSEKAKLASCNICTLDSSQLPGLEETLQGCGPKKELNLSTSPSPSYRSQGILPSGGAEDILWKWDALEAYQELVFGEDWHLSQQIASHVCQLTLRGNAVVQWQLYTHIFNPVLQRGVELTHHAQQLGVSTTCSQVCSYHTHCLPVEVLLVYLQTLPALLKSSRVVRDLFLSCNGLNQVTELIYLDCTRPWALKVFETLILNAWRNHTESAVHELERAEAQEREAVLGLVEELSTRGTGDAPQSLTKFYEGLKEACPPPKGKSETSEFSGVGRNRGEAHLNTINLFLCVAFLCVSKEADSDRDSANDSEDTSGYDSTASEPLGGRLPCLSPDSVALPSKEQVRRAADVWSVCRWVYMASPVFQRQFFRLGGLDVCSRLMTMVIQKLTSKNKDCKFKKKRESKGKSSPSREDSPTRCITGHFDNMVTNAGTAETQHPELSDTVSLSQTESKSSDPMHRLEEEWPFQSIRLLEALLAICLHSANSALQRLEPDLSFQLQSVEETLCEVRDQLSRSGVVNSELAVPLFDSLLRVALAEMFCSPEVIEEKPDKKCAVSEATPPAGDLSEDVDEAHVSAVQPLGEEEGYDADSESNPEDSTRQEEGLKVEAGVSLRECTLGMAGVPQGSVRGRLLFPEICLMELQLLASGSPDLEVLGHVLQSLLAAVKGHHNNAAQLYQQGGVKVILTGFPNILSQSDASYKECQTVLMELLVAMVSDRITAEELALLTRLFLEKTPPTEILLRGVLQIVEANTDTEPLYFMSFPMIPGSGGPGLSPSPGTRPHGATAGKGGVNTILRGKLPLGRGEADMSRPGHLRSSPWHAAPFHLPLVGQNCWPHMASGFSASLWVRAGKDEDGRSKKEDEVCVAEESPHGSAHVTEQQTERCLLHVLSMGSKALMLQVWVNISTRAFTFRICIDPNDEMKAGLLAQAESGDEVIPAGRWQHLALTYTQQPEGKKNIHGQLSLWVCGIRKCDVSLDFTLPRKSSLSSDSNKTYCMLGHYQHSAEEQASPSARWDMGTLMLFNGSRIDTTEAFYLYASGPDLTSIMPCKYGKPHGTLSKYVTQEGLQCEQIRELLMRNADVDISPLVESLAVVYTPSTPSLYTIYEPVIRLKGQAKSAVVSQRPFSAKEVQSSILESPFLRTMMPCQAFGLHSALHKIGGTGSVVFLFARAVELCDCEKTQALALQVLLSLVKNNQHRTHEMECYHGYSMIHQVLIKPKCIVGYHILKTLLDGCCSGSILTIGEDGQFHLDAESTVVVQDLRLLSEILLDWKIWAKAQCGVWETLLTALEILIRVRHPQQMFNIRQLLKAQVVHRFLLACQVLQEHRDEYLTSIPQEVCLSFVKIIQEVLGSPPDMDLLRLVYNFLLAVHPPTNTYVCHTPTSFYFSLHIDGKLYQEKVQSMMYLRHSSSGGKSACSSVVSLSPTVFTEMPAEVRHTSSIEHEDDLSLRPRSTGPSPHSSPALTPRLTHGRVTGTTEPEGGASLSTQQALGSTETLKRAGDEQLLSSCESAKTICDSPIAGADEEGSIMGNRTPSISVEEEQDGPAGSPVESEEHFDWTSEEAPRRPDSLKGIQSFQRSHSNLASLGLAFPAQNGSLTIARWPTVADRAAPPDDWESYTYSPGYDRHSKADSSNDRSSAEDCLVLICCGLYDLLRGVLLLLPDLLLEEVMDKLIQPDALLVLVNHPSPLIQQGVMKLLDAYFSRAQKEQKEKFLKNHGFSLLANQLYQHQGSQGLLECFLEMLFGRPVGLEEDLDLEDMENISPFRKRCIIPVLGLLENSLYENSLVHNGLCLLLQLLNACPKLADILLDHGLLYVLFNTLSTLNGLENGIPPNDYRLVVCDIQQLLVAVTIHSCSSSGSQYFRIIEDLITLLGYMQTSKVRRTQEMALALQFRVIQAAIDFIKTTANQDPQKSGSYVHVPTSPHHALQQKRKSIAGSMGLKDSIIPRIPRQHYCSYGLIPLPSPFSFLSQDSPLPSPTGSNSYTFPSDAGRRRFTLAQSDSLLTRMRSVASDELTQMMQRRMSQENPIRASETELIQRMQRLVVLAVNRLIYQDISHDFFDLLNFPESPDHSMSMPTAGDQTSDENGSTPSTPIPAGNIKSFRKDILRLMMEGIKIGLGSTGRGGAPRQQWRRILWSCRDTFRVQIGRMLVHTLSPELPLEDRKEALEFVHEQSYSEILRESLSPGLEHGPKLALYLYELLHDHKDALSREEQAAGNTFMTALKLCGHRCIPPSAPAKPDLLKAIKEEQLKYESEARTSKGAWEKKLSNTQRSLLQRLDGKSKDISKIAADITQNVSLRQGMERKKVMQHIRGLYKTDLSASRHWQELVQQLTHDRAVWYDQSSYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLKDRRKLDDSIKAPLAFLFEDKTHSSSSIVKDKATSEPIRFTRRCVSVAPSRETAGELLLGKSGMYFVEENVPETHDNQSPHGETEPASFSWTYEEIKEVHKRWWQLRDNAMEIFLTNGRTLLLAFDNTKFRDDVYHNILTSDLPNLLEYGNISALTHLWSSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILKDYTNETLDLQEASIYRNLSKPIAVQSKEKEDRYVDNYRYLEEEYKKGKREDDPMPPVQPYHYGSHYSNSGTVLHFLVRLPPFTKMFLAYQDQSFDIPDRTFHSMNTTWRLSSYESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNSERVNHVNLPPWARNDPRLFILIHRQALESDQVSQTLCQWIDLVFGLKQKGKAAVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFSASHISRAGTKLLSEAELPAAMGLLVQLAFRESREQPRDSGYPSPLPWIKGLKWGEYVGSPSAPDPVVCFSQPHGERFGSLLALPTRAICGLSRKFCLMMIYSKEQGVRSMHSTDIQWSAILSWGYTDNMLRLKSKQSEPPINFIQCSPLHQVSSCAWVPDSCQLFTGSKCGVITAYSNRFTTTMPTEMEVESQVDLYGHTAEVTGLFVCKPYSILISVSRDGTCILWDLNRLCYVQSLSGHKSPVNAVSASETTGDIATVCDSVGGGSDLRLWTINGDLIGHVHCREIICSVAFSNQPEGVSVNVIAGGLENGVVRLWSTWDLKPVREITFPKSNRPIISLTFSCDGHHLYTANSEGTVIAWCRRDQQRMKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_017358861.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358861.2

ORF Insert Sequence:

ATGGCCAGCT CACACCTGGC TGATCTGTCC CAGGCCTGGG AGAACTACAT GGACTGCCGC 
ACACAGGGCG CAGATCTGCA GGTCAGTCTT CAGTGCTTGG AGAGTTTTCT GTGCACGTTT 
CACTGTGTGC GACACCTCAG TGACCCCAGA GCAAAGGATG CGCTCAAATT CTGCTCGGAC 
ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
TCAATGGGGC TGAAAGACTC CATAATCCCC CGTATCCCCC GGCAGCACTA CTGCTCGTAC 
GGCCTGATCC CTCTGCCCTC TCCGTTCAGC TTCCTGTCCC AGGATTCCCC TCTCCCTTCC 
CCTACCGGGT CAAACTCCTA CACGTTCCCC TCAGACGCAG GTCGTAGACG CTTCACACTG 
GCCCAGTCGG ACTCTCTGCT GACCCGCATG CGTTCTGTAG CCAGTGATGA GCTGACACAG 
ATGATGCAGC GCAGGATGAG TCAAGAAAAC CCCATCAGAG CCAGCGAGAC CGAGCTCATC 
CAGAGAATGC AGAGACTGGT GGTCCTGGCT GTCAACAGAC TCATTTATCA AGACATCAGC 
CATGATTTCT TTGACCTGCT GAACTTCCCT GAGTCTCCTG ATCACTCGAT GTCCATGCCA 
ACAGCAGGAG ATCAGACATC TGATGAGAAC GGCTCCACTC CGTCTACCCC GATCCCAGCC 
GGAAACATCA AGAGCTTCCG GAAGGACATT CTCAGACTGA TGATGGAGGG CATAAAGATC 
GGCCTGGGCA GCACTGGTCG TGGCGGCGCT CCTCGTCAGC AGTGGCGGCG AATCCTCTGG 
TCCTGCCGAG ACACATTCAG AGTGCAGATC GGCCGCATGC TGGTGCACAC GCTCAGTCCT 
GAACTTCCTC TGGAGGACAG GAAGGAGGCG CTAGAGTTCG TCCATGAGCA GAGCTACTCC 
GAGATCCTCA GAGAAAGCCT CAGCCCCGGC CTCGAGCATG GGCCCAAGCT GGCTCTGTAT 
CTGTACGAGC TGCTGCACGA TCACAAAGAC GCCCTGAGTA GAGAAGAGCA GGCGGCAGGA 
AACACCTTCA TGACGGCTCT GAAACTCTGC GGACACCGCT GCATCCCACC CAGCGCTCCA 
GCCAAACCCG ACCTCCTGAA AGCCATCAAA GAGGAGCAAC TAAAGTATGA ATCAGAGGCA 
AGAACCAGCA AAGGAGCCTG GGAAAAGAAG CTGTCCAACA CACAGAGGAG CCTGTTGCAG 
AGGCTGGATG GCAAGTCGAA GGACATCTCG AAGATCGCGG CGGACATCAC TCAGAACGTG 
TCTCTCAGGC AGGGCATGGA GAGGAAGAAG GTCATGCAGC ACATTAGAGG CCTGTATAAG 
ACTGACCTGA GCGCTAGTCG ACACTGGCAG GAGCTCGTCC AGCAGCTCAC ACACGACCGG 
GCGGTGTGGT ACGATCAGTC CTCTTATCCC ACTTCCTGGC AGCTGGACCC CACCGAGGGC 
CCGAACCGAG AGCGGAGACG CCTCCAGCGC TGCTACCTGA CCATCCCCAA CAAATATCTG 
CTCAAAGACC GTCGCAAACT AGATGATTCC ATTAAGGCTC CTCTGGCCTT CCTGTTTGAG 
GACAAGACTC ACTCCTCTTC ATCTATAGTG AAGGATAAAG CCACCAGTGA GCCCATCAGG 
TTCACAAGAA GGTGCGTAAG TGTGGCTCCC TCCAGAGAGA CAGCAGGAGA GCTCTTACTG 
GGAAAATCTG GGATGTATTT TGTGGAGGAA AATGTTCCTG AGACTCACGA TAACCAGAGT 
CCTCATGGAG AGACGGAGCC GGCCTCTTTC TCCTGGACGT ATGAGGAGAT AAAGGAGGTT 
CACAAGCGCT GGTGGCAGCT CAGAGACAAC GCCATGGAGA TCTTCCTGAC CAATGGAAGG 
ACACTGCTGC TAGCCTTTGA CAACACAAAG TTTCGTGATG ACGTCTACCA CAACATCCTG 
ACCTCAGATC TGCCCAACCT GCTGGAGTAT GGAAACATCA GCGCCCTCAC GCACCTCTGG 
AGCTCAGGAC AGATCACCAA CTTTGAGTAC CTGACGCATC TCAACAAGCA CGCGGGACGC 
TCCTTCAATG ACCTCATGCA GTATCCCGTC TTCCCCTTCA TCCTCAAAGA TTACACCAAT 
GAGACGCTCG ACCTGCAGGA GGCCTCTATT TACAGGAACC TCAGCAAGCC CATTGCAGTG 
CAGTCTAAAG AGAAGGAAGA CCGTTATGTG GACAATTACA GGTATCTGGA GGAGGAGTAT 
AAGAAGGGCA AGAGAGAGGA CGACCCTATG CCCCCTGTCC AGCCCTATCA CTACGGCTCT 
CATTACTCCA ACAGTGGCAC CGTCCTGCAC TTCCTGGTCC GACTGCCTCC TTTTACCAAA 
ATGTTTCTGG CATATCAAGA CCAAAGCTTT GATATTCCTG ACCGAACCTT CCACTCCATG 
AACACCACGT GGCGTCTGTC GTCCTACGAG TCCATGACTG ATGTCAAAGA GCTCATCCCA 
GAGTTCTTCT ACCTGCCCGA GTTCCTGGTC AACAGAGAAG GTTTTGACTT TGGCGTGCGG 
CAGAACAGCG AGCGGGTCAA CCATGTGAAT CTGCCGCCGT GGGCCAGAAA TGACCCGCGA 
CTCTTCATCC TCATCCACAG ACAGGCTCTG GAGTCCGATC AAGTCTCGCA GACGCTCTGC 
CAGTGGATCG ACCTGGTGTT TGGACTCAAG CAGAAAGGCA AAGCTGCGGT CCAGGCCATC 
AACGTCTTTC ATCCTGCAAC GTACTTTGGT ATGGACGTGT CTGCAGTTGA AGACCCTGTG 
CAGCGGCGGG CTCTAGAAAC CATGATCAAG ACGTACGGAC AAACACCCAG GCAGCTGTTC 
AGCGCTTCTC ACATCAGCAG AGCCGGAACC AAACTGCTGT CAGAGGCTGA GCTGCCCGCC 
GCCATGGGTC TGCTGGTGCA GCTGGCCTTC AGAGAGAGCC GAGAACAGCC CCGGGACAGC 
GGCTACCCGA GTCCTCTGCC GTGGATAAAG GGCTTGAAAT GGGGCGAGTA TGTGGGTTCA 
CCCAGTGCTC CGGACCCTGT GGTCTGTTTC AGTCAACCTC ACGGCGAACG CTTCGGCTCC 
CTGCTGGCAC TGCCCACACG TGCCATCTGC GGCCTCTCAC GCAAGTTCTG CCTCATGATG 
ATCTACAGCA AAGAGCAAGG TGTGAGGAGC ATGCACAGCA CTGACATACA GTGGTCTGCT 
ATTCTGAGCT GGGGTTACAC CGACAACATG CTGCGTCTGA AGAGCAAACA GAGCGAACCA 
CCAATCAACT TCATCCAGTG CTCACCGCTG CACCAGGTGA GCAGTTGTGC CTGGGTGCCG 
GACAGTTGTC AGCTGTTTAC AGGCAGCAAG TGTGGAGTCA TTACCGCCTA CAGCAACCGC 
TTCACCACCA CCATGCCCAC AGAGATGGAG GTGGAGTCGC AGGTTGATCT GTACGGACAC 
ACAGCCGAGG TCACGGGTCT GTTCGTCTGT AAACCCTACA GTATCCTCAT CAGTGTGAGT 
CGGGACGGAA CCTGCATACT CTGGGATCTC AACAGGTTGT GTTACGTACA GAGTCTCAGC 
GGTCACAAGA GTCCAGTGAA CGCGGTGTCA GCCAGCGAGA CCACCGGAGA CATCGCCACC 
GTCTGCGACT CAGTGGGTGG AGGAAGTGAC CTGCGGCTGT GGACCATTAA TGGAGATCTG 
ATTGGTCACG TTCACTGTCG TGAGATCATC TGTTCAGTGG CTTTCTCCAA TCAGCCGGAG 
GGAGTTTCAG TCAATGTGAT CGCAGGCGGA CTGGAGAACG GGGTGGTCAG GTTGTGGAGC 
ACGTGGGACC TGAAGCCAGT CAGAGAGATC ACGTTCCCAA AGTCCAACAG ACCTATCATA 
AGTCTGACGT TCTCATGTGA CGGTCATCAT CTATACACGG CTAACAGTGA GGGCACGGTG 
ATCGCGTGGT GTCGGCGAGA CCAGCAGCGC ATGAAGCTGC CCATGTTTTA CTCTTTCCTC 
AGCAGTTACG CCGCAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lyst ( XM_017358862.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214351.1 Danio rerio lysosomal trafficking regulator (lyst), transcript variant X3, mRNA.

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CloneID	ODa54788
Clone ID Related Accession (Same CDS sequence)	XM_017358862.1 , XM_017358862.2
Accession Version	XM_017358862.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	lysosomal-trafficking regulator isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
TCAATGGGGC TGAAAGACTC CATAATCCCC CGTATCCCCC GGCAGCACTA CTGCTCGTAC 
GGCCTGATCC CTCTGCCCTC TCCGTTCAGC TTCCTGTCCC AGGATTCCCC TCTCCCTTCC 
CCTACCGGGT CAAACTCCTA CACGTTCCCC TCAGACGCAG GTCGTAGACG CTTCACACTG 
GCCCAGTCGG ACTCTCTGCT GACCCGCATG CGTTCTGTAG CCAGTGATGA GCTGACACAG 
ATGATGCAGC GCAGGATGAG TCAAGAAAAC CCCATCAGAG CCAGCGAGAC CGAGCTCATC 
CAGAGAATGC AGAGACTGGT GGTCCTGGCT GTCAACAGAC TCATTTATCA AGACATCAGC 
CATGATTTCT TTGACCTGCT GAACTTCCCT GAGTCTCCTG ATCACTCGAT GTCCATGCCA 
ACAGCAGGAG ATCAGACATC TGATGAGAAC GGCTCCACTC CGTCTACCCC GATCCCAGCC 
GGAAACATCA AGAGCTTCCG GAAGGACATT CTCAGACTGA TGATGGAGGG CATAAAGATC 
GGCCTGGGCA GCACTGGTCG TGGCGGCGCT CCTCGTCAGC AGTGGCGGCG AATCCTCTGG 
TCCTGCCGAG ACACATTCAG AGTGCAGATC GGCCGCATGC TGGTGCACAC GCTCAGTCCT 
GAACTTCCTC TGGAGGACAG GAAGGAGGCG CTAGAGTTCG TCCATGAGCA GAGCTACTCC 
GAGATCCTCA GAGAAAGCCT CAGCCCCGGC CTCGAGCATG GGCCCAAGCT GGCTCTGTAT 
CTGTACGAGC TGCTGCACGA TCACAAAGAC GCCCTGAGTA GAGAAGAGCA GGCGGCAGGA 
AACACCTTCA TGACGGCTCT GAAACTCTGC GGACACCGCT GCATCCCACC CAGCGCTCCA 
GCCAAACCCG ACCTCCTGAA AGCCATCAAA GAGGAGCAAC TAAAGTATGA ATCAGAGGCA 
AGAACCAGCA AAGGAGCCTG GGAAAAGAAG CTGTCCAACA CACAGAGGAG CCTGTTGCAG 
AGGCTGGATG GCAAGTCGAA GGACATCTCG AAGATCGCGG CGGACATCAC TCAGAACGTG 
TCTCTCAGGC AGGGCATGGA GAGGAAGAAG GTCATGCAGC ACATTAGAGG CCTGTATAAG 
ACTGACCTGA GCGCTAGTCG ACACTGGCAG GAGCTCGTCC AGCAGCTCAC ACACGACCGG 
GCGGTGTGGT ACGATCAGTC CTCTTATCCC ACTTCCTGGC AGCTGGACCC CACCGAGGGC 
CCGAACCGAG AGCGGAGACG CCTCCAGCGC TGCTACCTGA CCATCCCCAA CAAATATCTG 
CTCAAAGACC GTCGCAAACT AGATGATTCC ATTAAGGCTC CTCTGGCCTT CCTGTTTGAG 
GACAAGACTC ACTCCTCTTC ATCTATAGTG AAGGATAAAG CCACCAGTGA GCCCATCAGG 
TTCACAAGAA GGTGCGTAAG TGTGGCTCCC TCCAGAGAGA CAGCAGGAGA GCTCTTACTG 
GGAAAATCTG GGATGTATTT TGTGGAGGAA AATGTTCCTG AGACTCACGA TAACCAGAGT 
CCTCATGGAG AGACGGAGCC GGCCTCTTTC TCCTGGACGT ATGAGGAGAT AAAGGAGGTT 
CACAAGCGCT GGTGGCAGCT CAGAGACAAC GCCATGGAGA TCTTCCTGAC CAATGGAAGG 
ACACTGCTGC TAGCCTTTGA CAACACAAAG TTTCGTGATG ACGTCTACCA CAACATCCTG 
ACCTCAGATC TGCCCAACCT GCTGGAGTAT GGAAACATCA GCGCCCTCAC GCACCTCTGG 
AGCTCAGGAC AGATCACCAA CTTTGAGTAC CTGACGCATC TCAACAAGCA CGCGGGACGC 
TCCTTCAATG ACCTCATGCA GTATCCCGTC TTCCCCTTCA TCCTCAAAGA TTACACCAAT 
GAGACGCTCG ACCTGCAGGA GGCCTCTATT TACAGGAACC TCAGCAAGCC CATTGCAGTG 
CAGTCTAAAG AGAAGGAAGA CCGTTATGTG GACAATTACA GGTATCTGGA GGAGGAGTAT 
AAGAAGGGCA AGAGAGAGGA CGACCCTATG CCCCCTGTCC AGCCCTATCA CTACGGCTCT 
CATTACTCCA ACAGTGGCAC CGTCCTGCAC TTCCTGGTCC GACTGCCTCC TTTTACCAAA 
ATGTTTCTGG CATATCAAGA CCAAAGCTTT GATATTCCTG ACCGAACCTT CCACTCCATG 
AACACCACGT GGCGTCTGTC GTCCTACGAG TCCATGACTG ATGTCAAAGA GCTCATCCCA 
GAGTTCTTCT ACCTGCCCGA GTTCCTGGTC AACAGAGAAG GTTTTGACTT TGGCGTGCGG 
CAGAACAGCG AGCGGGTCAA CCATGTGAAT CTGCCGCCGT GGGCCAGAAA TGACCCGCGA 
CTCTTCATCC TCATCCACAG ACAGGCTCTG GAGTCCGATC AAGTCTCGCA GACGCTCTGC 
CAGTGGATCG ACCTGGTGTT TGGACTCAAG CAGAAAGGCA AAGCTGCGGT CCAGGCCATC 
AACGTCTTTC ATCCTGCAAC GTACTTTGGT ATGGACGTGT CTGCAGTTGA AGACCCTGTG 
CAGCGGCGGG CTCTAGAAAC CATGATCAAG ACGTACGGAC AAACACCCAG GCAGCTGTTC 
AGCGCTTCTC ACATCAGCAG AGCCGGAACC AAACTGCTGT CAGAGGCTGA GCTGCCCGCC 
GCCATGGGTC TGCTGGTGCA GCTGGCCTTC AGAGAGAGCC GAGAACAGCC CCGGGACAGC 
GGCTACCCGA GTCCTCTGCC GTGGATAAAG GGCTTGAAAT GGGGCGAGTA TGTGGGTTCA 
CCCAGTGCTC CGGACCCTGT GGTCTGTTTC AGTCAACCTC ACGGCGAACG CTTCGGCTCC 
CTGCTGGCAC TGCCCACACG TGCCATCTGC GGCCTCTCAC GCAAGTTCTG CCTCATGATG 
ATCTACAGCA AAGAGCAAGG TGTGAGGAGC ATGCACAGCA CTGACATACA GTGGTCTGCT 
ATTCTGAGCT GGGGTTACAC CGACAACATG CTGCGTCTGA AGAGCAAACA GAGCGAACCA 
CCAATCAACT TCATCCAGTG CTCACCGCTG CACCAGGTGA GCAGTTGTGC CTGGGTGCCG 
GACAGTTGTC AGCTGTTTAC AGGCAGCAAG TGTGGAGTCA TTACCGCCTA CAGCAACCGC 
TTCACCACCA CCATGCCCAC AGAGATGGAG GTGGAGTCGC AGGTTGATCT GTACGGACAC 
ACAGCCGAGG TCACGGGTCT GTTCGTCTGT AAACCCTACA GTATCCTCAT CAGTGTGAGT 
CGGGACGGAA CCTGCATACT CTGGGATCTC AACAGGTTGT GTTACGTACA GAGTCTCAGC 
GGTCACAAGA GTCCAGTGAA CGCGGTGTCA GCCAGCGAGA CCACCGGAGA CATCGCCACC 
GTCTGCGACT CAGTGGGTGG AGGAAGTGAC CTGCGGCTGT GGACCATTAA TGGAGATCTG 
ATTGGTCACG TTCACTGTCG TGAGATCATC TGTTCAGTGG CTTTCTCCAA TCAGCCGGAG 
GGAGTTTCAG TCAATGTGAT CGCAGGCGGA CTGGAGAACG GGGTGGTCAG GTTGTGGAGC 
ACGTGGGACC TGAAGCCAGT CAGAGAGATC ACGTTCCCAA AGTCCAACAG ACCTATCATA 
AGTCTGACGT TCTCATGTGA CGGTCATCAT CTATACACGG CTAACAGTGA GGGCACGGTG 
ATCGCGTGGT GTCGGCGAGA CCAGCAGCGC ATGAAGCTGC CCATGTTTTA CTCTTTCCTC 
AGCAGTTACG CCGCAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214351.1
CDS	125..11605
Translation	MSGASNTLAREFLTDVHQLCSAVAQRAEDEEESHMVALGEYLVRGRGFLLLSTLESVIDQELTCREELLTLLLSLLPLVWKIPVQEEKAPDFTLPSLLEVFLCQEGRTISQKAGQDPCDVQNSTKRLSGSWKSRRSRRTAQRYSVRDARKSQLSTSDSEANSDDKTTNTGSKHRRFHSTAIHVSCQLHQSNNSSQPAPQHPTLDAMGTFEPSQPHLRLFGSHTMDPNMLSDPTTLSIFNRMENSPFDLCHVLLSLLEKVCKFDMTINHNPGLAVSVVPTLTEILTDFGDCCGPSGGGGGGIDELAGGWTEEPIALVQRMLLRTILHLMFVDVGQNETLPDNLRRSLTDLLRATLKIRTCLERQADPFAPRPKKTLQEIQEDFSFSRYRHRALLLPELLQGVLQLLLGCLQASAPNPFFFSQSLELVHEFVQHRGLELFEVTALRLEALAEAHNSEISNEASEHLRCLIADVLKIISAVKRAKSEQLHQSVCARRRHRRCEYSHFLHHHRDLSGLPVSAFKQAARRNPFEEDSKGNDGEVRYPERCCCLAACAHQCLRLFQRLSPSGPAVLQVLAGVQAVGICCCMDPRSVIAPMLQAFQAPGLRSYQSHVLSVLSRLVLEQLGGGQPSEKAKLASCNICTLDSSQLPGLEETLQGCGPKKELNLSTSPSPSYRSQGILPSGGAEDILWKWDALEAYQELVFGEDWHLSQQIASHVCQLTLRGNAVVQWQLYTHIFNPVLQRGVELTHHAQQLGVSTTCSQVCSYHTHCLPVEVLLVYLQTLPALLKSSRVVRDLFLSCNGLNQVTELIYLDCTRPWALKVFETLILNAWRNHTESAVHELERAEAQEREAVLGLVEELSTRGTGDAPQSLTKFYEGLKEACPPPKGKSETSEFSGVGRNRGEAHLNTINLFLCVAFLCVSKEADSDRDSANDSEDTSGYDSTASEPLGGRLPCLSPDSVALPSKEQVRRAADVWSVCRWVYMASPVFQRQFFRLGGLDVCSRLMTMVIQKLTSKNKDCKFKKKRESKGKSSPSREDSPTRCITGHFDNMVTNAGTAETQHPELSDTVSLSQTESKSSDPMHRLEEEWPFQSIRLLEALLAICLHSANSALQRLEPDLSFQLQSVEETLCEVRDQLSRSGVVNSELAVPLFDSLLRVALAEMFCSPEVIEEKPDKKCAVSEATPPAGDLSEDVDEAHVSAVQPLGEEEGYDADSESNPEDSTRQEEGLKVEAGVSLRECTLGMAGVPQGSVRGRLLFPEICLMELQLLASGSPDLEVLGHVLQSLLAAVKGHHNNAAQLYQQGGVKVILTGFPNILSQSDASYKECQTVLMELLVAMVSDRITAEELALLTRLFLEKTPPTEILLRGVLQIVEANTDTEPLYFMSFPMIPGSGGPGLSPSPGTRPHGATAGKGGVNTILRGKLPLGRGEADMSRPGHLRSSPWHAAPFHLPLVGQNCWPHMASGFSASLWVRAGKDEDGRSKKEDEVCVAEESPHGSAHVTEQQTERCLLHVLSMGSKALMLQVWVNISTRAFTFRICIDPNDEMKAGLLAQAESGDEVIPAGRWQHLALTYTQQPEGKKNIHGQLSLWVCGIRKCDVSLDFTLPRKSSLSSDSNKTYCMLGHYQHSAEEQASPSARWDMGTLMLFNGSRIDTTEAFYLYASGPDLTSIMPCKYGKPHGTLSKYVTQEGLQCEQIRELLMRNADVDISPLVESLAVVYTPSTPSLYTIYEPVIRLKGQAKSAVVSQRPFSAKEVQSSILESPFLRTMMPCQAFGLHSALHKIGGTGSVVFLFARAVELCDCEKTQALALQVLLSLVKNNQHRTHEMECYHGYSMIHQVLIKPKCIVGYHILKTLLDGCCSGSILTIGEDGQFHLDAESTVVVQDLRLLSEILLDWKIWAKAQCGVWETLLTALEILIRVRHPQQMFNIRQLLKAQVVHRFLLACQVLQEHRDEYLTSIPQEVCLSFVKIIQEVLGSPPDMDLLRLVYNFLLAVHPPTNTYVCHTPTSFYFSLHIDGKLYQEKVQSMMYLRHSSSGGKSACSSVVSLSPTVFTEMPAEVRHTSSIEHEDDLSLRPRSTGPSPHSSPALTPRLTHGRVTGTTEPEGGASLSTQQALGSTETLKRAGDEQLLSSCESAKTICDSPIAGADEEGSIMGNRTPSISVEEEQDGPAGSPVESEEHFDWTSEEAPRRPDSLKGIQSFQRSHSNLASLGLAFPAQNGSLTIARWPTVADRAAPPDDWESYTYSPGYDRHSKADSSNDRSSAEDCLVLICCGLYDLLRGVLLLLPDLLLEEVMDKLIQPDALLVLVNHPSPLIQQGVMKLLDAYFSRAQKEQKEKFLKNHGFSLLANQLYQHQGSQGLLECFLEMLFGRPVGLEEDLDLEDMENISPFRKRCIIPVLGLLENSLYENSLVHNGLCLLLQLLNACPKLADILLDHGLLYVLFNTLSTLNGLENGIPPNDYRLVVCDIQQLLVAVTIHSCSSSGSQYFRIIEDLITLLGYMQTSKVRRTQEMALALQFRVIQAAIDFIKTTANQDPQKSGSYVHVPTSPHHALQQKRKSIAGSMGLKDSIIPRIPRQHYCSYGLIPLPSPFSFLSQDSPLPSPTGSNSYTFPSDAGRRRFTLAQSDSLLTRMRSVASDELTQMMQRRMSQENPIRASETELIQRMQRLVVLAVNRLIYQDISHDFFDLLNFPESPDHSMSMPTAGDQTSDENGSTPSTPIPAGNIKSFRKDILRLMMEGIKIGLGSTGRGGAPRQQWRRILWSCRDTFRVQIGRMLVHTLSPELPLEDRKEALEFVHEQSYSEILRESLSPGLEHGPKLALYLYELLHDHKDALSREEQAAGNTFMTALKLCGHRCIPPSAPAKPDLLKAIKEEQLKYESEARTSKGAWEKKLSNTQRSLLQRLDGKSKDISKIAADITQNVSLRQGMERKKVMQHIRGLYKTDLSASRHWQELVQQLTHDRAVWYDQSSYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLKDRRKLDDSIKAPLAFLFEDKTHSSSSIVKDKATSEPIRFTRRCVSVAPSRETAGELLLGKSGMYFVEENVPETHDNQSPHGETEPASFSWTYEEIKEVHKRWWQLRDNAMEIFLTNGRTLLLAFDNTKFRDDVYHNILTSDLPNLLEYGNISALTHLWSSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILKDYTNETLDLQEASIYRNLSKPIAVQSKEKEDRYVDNYRYLEEEYKKGKREDDPMPPVQPYHYGSHYSNSGTVLHFLVRLPPFTKMFLAYQDQSFDIPDRTFHSMNTTWRLSSYESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNSERVNHVNLPPWARNDPRLFILIHRQALESDQVSQTLCQWIDLVFGLKQKGKAAVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFSASHISRAGTKLLSEAELPAAMGLLVQLAFRESREQPRDSGYPSPLPWIKGLKWGEYVGSPSAPDPVVCFSQPHGERFGSLLALPTRAICGLSRKFCLMMIYSKEQGVRSMHSTDIQWSAILSWGYTDNMLRLKSKQSEPPINFIQCSPLHQVSSCAWVPDSCQLFTGSKCGVITAYSNRFTTTMPTEMEVESQVDLYGHTAEVTGLFVCKPYSILISVSRDGTCILWDLNRLCYVQSLSGHKSPVNAVSASETTGDIATVCDSVGGGSDLRLWTINGDLIGHVHCREIICSVAFSNQPEGVSVNVIAGGLENGVVRLWSTWDLKPVREITFPKSNRPIISLTFSCDGHHLYTANSEGTVIAWCRRDQQRMKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_017358862.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358862.1

ORF Insert Sequence:

ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
TCAATGGGGC TGAAAGACTC CATAATCCCC CGTATCCCCC GGCAGCACTA CTGCTCGTAC 
GGCCTGATCC CTCTGCCCTC TCCGTTCAGC TTCCTGTCCC AGGATTCCCC TCTCCCTTCC 
CCTACCGGGT CAAACTCCTA CACGTTCCCC TCAGACGCAG GTCGTAGACG CTTCACACTG 
GCCCAGTCGG ACTCTCTGCT GACCCGCATG CGTTCTGTAG CCAGTGATGA GCTGACACAG 
ATGATGCAGC GCAGGATGAG TCAAGAAAAC CCCATCAGAG CCAGCGAGAC CGAGCTCATC 
CAGAGAATGC AGAGACTGGT GGTCCTGGCT GTCAACAGAC TCATTTATCA AGACATCAGC 
CATGATTTCT TTGACCTGCT GAACTTCCCT GAGTCTCCTG ATCACTCGAT GTCCATGCCA 
ACAGCAGGAG ATCAGACATC TGATGAGAAC GGCTCCACTC CGTCTACCCC GATCCCAGCC 
GGAAACATCA AGAGCTTCCG GAAGGACATT CTCAGACTGA TGATGGAGGG CATAAAGATC 
GGCCTGGGCA GCACTGGTCG TGGCGGCGCT CCTCGTCAGC AGTGGCGGCG AATCCTCTGG 
TCCTGCCGAG ACACATTCAG AGTGCAGATC GGCCGCATGC TGGTGCACAC GCTCAGTCCT 
GAACTTCCTC TGGAGGACAG GAAGGAGGCG CTAGAGTTCG TCCATGAGCA GAGCTACTCC 
GAGATCCTCA GAGAAAGCCT CAGCCCCGGC CTCGAGCATG GGCCCAAGCT GGCTCTGTAT 
CTGTACGAGC TGCTGCACGA TCACAAAGAC GCCCTGAGTA GAGAAGAGCA GGCGGCAGGA 
AACACCTTCA TGACGGCTCT GAAACTCTGC GGACACCGCT GCATCCCACC CAGCGCTCCA 
GCCAAACCCG ACCTCCTGAA AGCCATCAAA GAGGAGCAAC TAAAGTATGA ATCAGAGGCA 
AGAACCAGCA AAGGAGCCTG GGAAAAGAAG CTGTCCAACA CACAGAGGAG CCTGTTGCAG 
AGGCTGGATG GCAAGTCGAA GGACATCTCG AAGATCGCGG CGGACATCAC TCAGAACGTG 
TCTCTCAGGC AGGGCATGGA GAGGAAGAAG GTCATGCAGC ACATTAGAGG CCTGTATAAG 
ACTGACCTGA GCGCTAGTCG ACACTGGCAG GAGCTCGTCC AGCAGCTCAC ACACGACCGG 
GCGGTGTGGT ACGATCAGTC CTCTTATCCC ACTTCCTGGC AGCTGGACCC CACCGAGGGC 
CCGAACCGAG AGCGGAGACG CCTCCAGCGC TGCTACCTGA CCATCCCCAA CAAATATCTG 
CTCAAAGACC GTCGCAAACT AGATGATTCC ATTAAGGCTC CTCTGGCCTT CCTGTTTGAG 
GACAAGACTC ACTCCTCTTC ATCTATAGTG AAGGATAAAG CCACCAGTGA GCCCATCAGG 
TTCACAAGAA GGTGCGTAAG TGTGGCTCCC TCCAGAGAGA CAGCAGGAGA GCTCTTACTG 
GGAAAATCTG GGATGTATTT TGTGGAGGAA AATGTTCCTG AGACTCACGA TAACCAGAGT 
CCTCATGGAG AGACGGAGCC GGCCTCTTTC TCCTGGACGT ATGAGGAGAT AAAGGAGGTT 
CACAAGCGCT GGTGGCAGCT CAGAGACAAC GCCATGGAGA TCTTCCTGAC CAATGGAAGG 
ACACTGCTGC TAGCCTTTGA CAACACAAAG TTTCGTGATG ACGTCTACCA CAACATCCTG 
ACCTCAGATC TGCCCAACCT GCTGGAGTAT GGAAACATCA GCGCCCTCAC GCACCTCTGG 
AGCTCAGGAC AGATCACCAA CTTTGAGTAC CTGACGCATC TCAACAAGCA CGCGGGACGC 
TCCTTCAATG ACCTCATGCA GTATCCCGTC TTCCCCTTCA TCCTCAAAGA TTACACCAAT 
GAGACGCTCG ACCTGCAGGA GGCCTCTATT TACAGGAACC TCAGCAAGCC CATTGCAGTG 
CAGTCTAAAG AGAAGGAAGA CCGTTATGTG GACAATTACA GGTATCTGGA GGAGGAGTAT 
AAGAAGGGCA AGAGAGAGGA CGACCCTATG CCCCCTGTCC AGCCCTATCA CTACGGCTCT 
CATTACTCCA ACAGTGGCAC CGTCCTGCAC TTCCTGGTCC GACTGCCTCC TTTTACCAAA 
ATGTTTCTGG CATATCAAGA CCAAAGCTTT GATATTCCTG ACCGAACCTT CCACTCCATG 
AACACCACGT GGCGTCTGTC GTCCTACGAG TCCATGACTG ATGTCAAAGA GCTCATCCCA 
GAGTTCTTCT ACCTGCCCGA GTTCCTGGTC AACAGAGAAG GTTTTGACTT TGGCGTGCGG 
CAGAACAGCG AGCGGGTCAA CCATGTGAAT CTGCCGCCGT GGGCCAGAAA TGACCCGCGA 
CTCTTCATCC TCATCCACAG ACAGGCTCTG GAGTCCGATC AAGTCTCGCA GACGCTCTGC 
CAGTGGATCG ACCTGGTGTT TGGACTCAAG CAGAAAGGCA AAGCTGCGGT CCAGGCCATC 
AACGTCTTTC ATCCTGCAAC GTACTTTGGT ATGGACGTGT CTGCAGTTGA AGACCCTGTG 
CAGCGGCGGG CTCTAGAAAC CATGATCAAG ACGTACGGAC AAACACCCAG GCAGCTGTTC 
AGCGCTTCTC ACATCAGCAG AGCCGGAACC AAACTGCTGT CAGAGGCTGA GCTGCCCGCC 
GCCATGGGTC TGCTGGTGCA GCTGGCCTTC AGAGAGAGCC GAGAACAGCC CCGGGACAGC 
GGCTACCCGA GTCCTCTGCC GTGGATAAAG GGCTTGAAAT GGGGCGAGTA TGTGGGTTCA 
CCCAGTGCTC CGGACCCTGT GGTCTGTTTC AGTCAACCTC ACGGCGAACG CTTCGGCTCC 
CTGCTGGCAC TGCCCACACG TGCCATCTGC GGCCTCTCAC GCAAGTTCTG CCTCATGATG 
ATCTACAGCA AAGAGCAAGG TGTGAGGAGC ATGCACAGCA CTGACATACA GTGGTCTGCT 
ATTCTGAGCT GGGGTTACAC CGACAACATG CTGCGTCTGA AGAGCAAACA GAGCGAACCA 
CCAATCAACT TCATCCAGTG CTCACCGCTG CACCAGGTGA GCAGTTGTGC CTGGGTGCCG 
GACAGTTGTC AGCTGTTTAC AGGCAGCAAG TGTGGAGTCA TTACCGCCTA CAGCAACCGC 
TTCACCACCA CCATGCCCAC AGAGATGGAG GTGGAGTCGC AGGTTGATCT GTACGGACAC 
ACAGCCGAGG TCACGGGTCT GTTCGTCTGT AAACCCTACA GTATCCTCAT CAGTGTGAGT 
CGGGACGGAA CCTGCATACT CTGGGATCTC AACAGGTTGT GTTACGTACA GAGTCTCAGC 
GGTCACAAGA GTCCAGTGAA CGCGGTGTCA GCCAGCGAGA CCACCGGAGA CATCGCCACC 
GTCTGCGACT CAGTGGGTGG AGGAAGTGAC CTGCGGCTGT GGACCATTAA TGGAGATCTG 
ATTGGTCACG TTCACTGTCG TGAGATCATC TGTTCAGTGG CTTTCTCCAA TCAGCCGGAG 
GGAGTTTCAG TCAATGTGAT CGCAGGCGGA CTGGAGAACG GGGTGGTCAG GTTGTGGAGC 
ACGTGGGACC TGAAGCCAGT CAGAGAGATC ACGTTCCCAA AGTCCAACAG ACCTATCATA 
AGTCTGACGT TCTCATGTGA CGGTCATCAT CTATACACGG CTAACAGTGA GGGCACGGTG 
ATCGCGTGGT GTCGGCGAGA CCAGCAGCGC ATGAAGCTGC CCATGTTTTA CTCTTTCCTC 
AGCAGTTACG CCGCAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lyst ( XM_017358862.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214351.1 Danio rerio lysosomal trafficking regulator (lyst), transcript variant X3, mRNA.

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CloneID	ODa54788
Clone ID Related Accession (Same CDS sequence)	XM_017358862.1 , XM_017358862.2
Accession Version	XM_017358862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lysosomal-trafficking regulator isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
TCAATGGGGC TGAAAGACTC CATAATCCCC CGTATCCCCC GGCAGCACTA CTGCTCGTAC 
GGCCTGATCC CTCTGCCCTC TCCGTTCAGC TTCCTGTCCC AGGATTCCCC TCTCCCTTCC 
CCTACCGGGT CAAACTCCTA CACGTTCCCC TCAGACGCAG GTCGTAGACG CTTCACACTG 
GCCCAGTCGG ACTCTCTGCT GACCCGCATG CGTTCTGTAG CCAGTGATGA GCTGACACAG 
ATGATGCAGC GCAGGATGAG TCAAGAAAAC CCCATCAGAG CCAGCGAGAC CGAGCTCATC 
CAGAGAATGC AGAGACTGGT GGTCCTGGCT GTCAACAGAC TCATTTATCA AGACATCAGC 
CATGATTTCT TTGACCTGCT GAACTTCCCT GAGTCTCCTG ATCACTCGAT GTCCATGCCA 
ACAGCAGGAG ATCAGACATC TGATGAGAAC GGCTCCACTC CGTCTACCCC GATCCCAGCC 
GGAAACATCA AGAGCTTCCG GAAGGACATT CTCAGACTGA TGATGGAGGG CATAAAGATC 
GGCCTGGGCA GCACTGGTCG TGGCGGCGCT CCTCGTCAGC AGTGGCGGCG AATCCTCTGG 
TCCTGCCGAG ACACATTCAG AGTGCAGATC GGCCGCATGC TGGTGCACAC GCTCAGTCCT 
GAACTTCCTC TGGAGGACAG GAAGGAGGCG CTAGAGTTCG TCCATGAGCA GAGCTACTCC 
GAGATCCTCA GAGAAAGCCT CAGCCCCGGC CTCGAGCATG GGCCCAAGCT GGCTCTGTAT 
CTGTACGAGC TGCTGCACGA TCACAAAGAC GCCCTGAGTA GAGAAGAGCA GGCGGCAGGA 
AACACCTTCA TGACGGCTCT GAAACTCTGC GGACACCGCT GCATCCCACC CAGCGCTCCA 
GCCAAACCCG ACCTCCTGAA AGCCATCAAA GAGGAGCAAC TAAAGTATGA ATCAGAGGCA 
AGAACCAGCA AAGGAGCCTG GGAAAAGAAG CTGTCCAACA CACAGAGGAG CCTGTTGCAG 
AGGCTGGATG GCAAGTCGAA GGACATCTCG AAGATCGCGG CGGACATCAC TCAGAACGTG 
TCTCTCAGGC AGGGCATGGA GAGGAAGAAG GTCATGCAGC ACATTAGAGG CCTGTATAAG 
ACTGACCTGA GCGCTAGTCG ACACTGGCAG GAGCTCGTCC AGCAGCTCAC ACACGACCGG 
GCGGTGTGGT ACGATCAGTC CTCTTATCCC ACTTCCTGGC AGCTGGACCC CACCGAGGGC 
CCGAACCGAG AGCGGAGACG CCTCCAGCGC TGCTACCTGA CCATCCCCAA CAAATATCTG 
CTCAAAGACC GTCGCAAACT AGATGATTCC ATTAAGGCTC CTCTGGCCTT CCTGTTTGAG 
GACAAGACTC ACTCCTCTTC ATCTATAGTG AAGGATAAAG CCACCAGTGA GCCCATCAGG 
TTCACAAGAA GGTGCGTAAG TGTGGCTCCC TCCAGAGAGA CAGCAGGAGA GCTCTTACTG 
GGAAAATCTG GGATGTATTT TGTGGAGGAA AATGTTCCTG AGACTCACGA TAACCAGAGT 
CCTCATGGAG AGACGGAGCC GGCCTCTTTC TCCTGGACGT ATGAGGAGAT AAAGGAGGTT 
CACAAGCGCT GGTGGCAGCT CAGAGACAAC GCCATGGAGA TCTTCCTGAC CAATGGAAGG 
ACACTGCTGC TAGCCTTTGA CAACACAAAG TTTCGTGATG ACGTCTACCA CAACATCCTG 
ACCTCAGATC TGCCCAACCT GCTGGAGTAT GGAAACATCA GCGCCCTCAC GCACCTCTGG 
AGCTCAGGAC AGATCACCAA CTTTGAGTAC CTGACGCATC TCAACAAGCA CGCGGGACGC 
TCCTTCAATG ACCTCATGCA GTATCCCGTC TTCCCCTTCA TCCTCAAAGA TTACACCAAT 
GAGACGCTCG ACCTGCAGGA GGCCTCTATT TACAGGAACC TCAGCAAGCC CATTGCAGTG 
CAGTCTAAAG AGAAGGAAGA CCGTTATGTG GACAATTACA GGTATCTGGA GGAGGAGTAT 
AAGAAGGGCA AGAGAGAGGA CGACCCTATG CCCCCTGTCC AGCCCTATCA CTACGGCTCT 
CATTACTCCA ACAGTGGCAC CGTCCTGCAC TTCCTGGTCC GACTGCCTCC TTTTACCAAA 
ATGTTTCTGG CATATCAAGA CCAAAGCTTT GATATTCCTG ACCGAACCTT CCACTCCATG 
AACACCACGT GGCGTCTGTC GTCCTACGAG TCCATGACTG ATGTCAAAGA GCTCATCCCA 
GAGTTCTTCT ACCTGCCCGA GTTCCTGGTC AACAGAGAAG GTTTTGACTT TGGCGTGCGG 
CAGAACAGCG AGCGGGTCAA CCATGTGAAT CTGCCGCCGT GGGCCAGAAA TGACCCGCGA 
CTCTTCATCC TCATCCACAG ACAGGCTCTG GAGTCCGATC AAGTCTCGCA GACGCTCTGC 
CAGTGGATCG ACCTGGTGTT TGGACTCAAG CAGAAAGGCA AAGCTGCGGT CCAGGCCATC 
AACGTCTTTC ATCCTGCAAC GTACTTTGGT ATGGACGTGT CTGCAGTTGA AGACCCTGTG 
CAGCGGCGGG CTCTAGAAAC CATGATCAAG ACGTACGGAC AAACACCCAG GCAGCTGTTC 
AGCGCTTCTC ACATCAGCAG AGCCGGAACC AAACTGCTGT CAGAGGCTGA GCTGCCCGCC 
GCCATGGGTC TGCTGGTGCA GCTGGCCTTC AGAGAGAGCC GAGAACAGCC CCGGGACAGC 
GGCTACCCGA GTCCTCTGCC GTGGATAAAG GGCTTGAAAT GGGGCGAGTA TGTGGGTTCA 
CCCAGTGCTC CGGACCCTGT GGTCTGTTTC AGTCAACCTC ACGGCGAACG CTTCGGCTCC 
CTGCTGGCAC TGCCCACACG TGCCATCTGC GGCCTCTCAC GCAAGTTCTG CCTCATGATG 
ATCTACAGCA AAGAGCAAGG TGTGAGGAGC ATGCACAGCA CTGACATACA GTGGTCTGCT 
ATTCTGAGCT GGGGTTACAC CGACAACATG CTGCGTCTGA AGAGCAAACA GAGCGAACCA 
CCAATCAACT TCATCCAGTG CTCACCGCTG CACCAGGTGA GCAGTTGTGC CTGGGTGCCG 
GACAGTTGTC AGCTGTTTAC AGGCAGCAAG TGTGGAGTCA TTACCGCCTA CAGCAACCGC 
TTCACCACCA CCATGCCCAC AGAGATGGAG GTGGAGTCGC AGGTTGATCT GTACGGACAC 
ACAGCCGAGG TCACGGGTCT GTTCGTCTGT AAACCCTACA GTATCCTCAT CAGTGTGAGT 
CGGGACGGAA CCTGCATACT CTGGGATCTC AACAGGTTGT GTTACGTACA GAGTCTCAGC 
GGTCACAAGA GTCCAGTGAA CGCGGTGTCA GCCAGCGAGA CCACCGGAGA CATCGCCACC 
GTCTGCGACT CAGTGGGTGG AGGAAGTGAC CTGCGGCTGT GGACCATTAA TGGAGATCTG 
ATTGGTCACG TTCACTGTCG TGAGATCATC TGTTCAGTGG CTTTCTCCAA TCAGCCGGAG 
GGAGTTTCAG TCAATGTGAT CGCAGGCGGA CTGGAGAACG GGGTGGTCAG GTTGTGGAGC 
ACGTGGGACC TGAAGCCAGT CAGAGAGATC ACGTTCCCAA AGTCCAACAG ACCTATCATA 
AGTCTGACGT TCTCATGTGA CGGTCATCAT CTATACACGG CTAACAGTGA GGGCACGGTG 
ATCGCGTGGT GTCGGCGAGA CCAGCAGCGC ATGAAGCTGC CCATGTTTTA CTCTTTCCTC 
AGCAGTTACG CCGCAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214351.1
CDS	301..11781
Translation	MSGASNTLAREFLTDVHQLCSAVAQRAEDEEESHMVALGEYLVRGRGFLLLSTLESVIDQELTCREELLTLLLSLLPLVWKIPVQEEKAPDFTLPSLLEVFLCQEGRTISQKAGQDPCDVQNSTKRLSGSWKSRRSRRTAQRYSVRDARKSQLSTSDSEANSDDKTTNTGSKHRRFHSTAIHVSCQLHQSNNSSQPAPQHPTLDAMGTFEPSQPHLRLFGSHTMDPNMLSDPTTLSIFNRMENSPFDLCHVLLSLLEKVCKFDMTINHNPGLAVSVVPTLTEILTDFGDCCGPSGGGGGGIDELAGGWTEEPIALVQRMLLRTILHLMFVDVGQNETLPDNLRRSLTDLLRATLKIRTCLERQADPFAPRPKKTLQEIQEDFSFSRYRHRALLLPELLQGVLQLLLGCLQASAPNPFFFSQSLELVHEFVQHRGLELFEVTALRLEALAEAHNSEISNEASEHLRCLIADVLKIISAVKRAKSEQLHQSVCARRRHRRCEYSHFLHHHRDLSGLPVSAFKQAARRNPFEEDSKGNDGEVRYPERCCCLAACAHQCLRLFQRLSPSGPAVLQVLAGVQAVGICCCMDPRSVIAPMLQAFQAPGLRSYQSHVLSVLSRLVLEQLGGGQPSEKAKLASCNICTLDSSQLPGLEETLQGCGPKKELNLSTSPSPSYRSQGILPSGGAEDILWKWDALEAYQELVFGEDWHLSQQIASHVCQLTLRGNAVVQWQLYTHIFNPVLQRGVELTHHAQQLGVSTTCSQVCSYHTHCLPVEVLLVYLQTLPALLKSSRVVRDLFLSCNGLNQVTELIYLDCTRPWALKVFETLILNAWRNHTESAVHELERAEAQEREAVLGLVEELSTRGTGDAPQSLTKFYEGLKEACPPPKGKSETSEFSGVGRNRGEAHLNTINLFLCVAFLCVSKEADSDRDSANDSEDTSGYDSTASEPLGGRLPCLSPDSVALPSKEQVRRAADVWSVCRWVYMASPVFQRQFFRLGGLDVCSRLMTMVIQKLTSKNKDCKFKKKRESKGKSSPSREDSPTRCITGHFDNMVTNAGTAETQHPELSDTVSLSQTESKSSDPMHRLEEEWPFQSIRLLEALLAICLHSANSALQRLEPDLSFQLQSVEETLCEVRDQLSRSGVVNSELAVPLFDSLLRVALAEMFCSPEVIEEKPDKKCAVSEATPPAGDLSEDVDEAHVSAVQPLGEEEGYDADSESNPEDSTRQEEGLKVEAGVSLRECTLGMAGVPQGSVRGRLLFPEICLMELQLLASGSPDLEVLGHVLQSLLAAVKGHHNNAAQLYQQGGVKVILTGFPNILSQSDASYKECQTVLMELLVAMVSDRITAEELALLTRLFLEKTPPTEILLRGVLQIVEANTDTEPLYFMSFPMIPGSGGPGLSPSPGTRPHGATAGKGGVNTILRGKLPLGRGEADMSRPGHLRSSPWHAAPFHLPLVGQNCWPHMASGFSASLWVRAGKDEDGRSKKEDEVCVAEESPHGSAHVTEQQTERCLLHVLSMGSKALMLQVWVNISTRAFTFRICIDPNDEMKAGLLAQAESGDEVIPAGRWQHLALTYTQQPEGKKNIHGQLSLWVCGIRKCDVSLDFTLPRKSSLSSDSNKTYCMLGHYQHSAEEQASPSARWDMGTLMLFNGSRIDTTEAFYLYASGPDLTSIMPCKYGKPHGTLSKYVTQEGLQCEQIRELLMRNADVDISPLVESLAVVYTPSTPSLYTIYEPVIRLKGQAKSAVVSQRPFSAKEVQSSILESPFLRTMMPCQAFGLHSALHKIGGTGSVVFLFARAVELCDCEKTQALALQVLLSLVKNNQHRTHEMECYHGYSMIHQVLIKPKCIVGYHILKTLLDGCCSGSILTIGEDGQFHLDAESTVVVQDLRLLSEILLDWKIWAKAQCGVWETLLTALEILIRVRHPQQMFNIRQLLKAQVVHRFLLACQVLQEHRDEYLTSIPQEVCLSFVKIIQEVLGSPPDMDLLRLVYNFLLAVHPPTNTYVCHTPTSFYFSLHIDGKLYQEKVQSMMYLRHSSSGGKSACSSVVSLSPTVFTEMPAEVRHTSSIEHEDDLSLRPRSTGPSPHSSPALTPRLTHGRVTGTTEPEGGASLSTQQALGSTETLKRAGDEQLLSSCESAKTICDSPIAGADEEGSIMGNRTPSISVEEEQDGPAGSPVESEEHFDWTSEEAPRRPDSLKGIQSFQRSHSNLASLGLAFPAQNGSLTIARWPTVADRAAPPDDWESYTYSPGYDRHSKADSSNDRSSAEDCLVLICCGLYDLLRGVLLLLPDLLLEEVMDKLIQPDALLVLVNHPSPLIQQGVMKLLDAYFSRAQKEQKEKFLKNHGFSLLANQLYQHQGSQGLLECFLEMLFGRPVGLEEDLDLEDMENISPFRKRCIIPVLGLLENSLYENSLVHNGLCLLLQLLNACPKLADILLDHGLLYVLFNTLSTLNGLENGIPPNDYRLVVCDIQQLLVAVTIHSCSSSGSQYFRIIEDLITLLGYMQTSKVRRTQEMALALQFRVIQAAIDFIKTTANQDPQKSGSYVHVPTSPHHALQQKRKSIAGSMGLKDSIIPRIPRQHYCSYGLIPLPSPFSFLSQDSPLPSPTGSNSYTFPSDAGRRRFTLAQSDSLLTRMRSVASDELTQMMQRRMSQENPIRASETELIQRMQRLVVLAVNRLIYQDISHDFFDLLNFPESPDHSMSMPTAGDQTSDENGSTPSTPIPAGNIKSFRKDILRLMMEGIKIGLGSTGRGGAPRQQWRRILWSCRDTFRVQIGRMLVHTLSPELPLEDRKEALEFVHEQSYSEILRESLSPGLEHGPKLALYLYELLHDHKDALSREEQAAGNTFMTALKLCGHRCIPPSAPAKPDLLKAIKEEQLKYESEARTSKGAWEKKLSNTQRSLLQRLDGKSKDISKIAADITQNVSLRQGMERKKVMQHIRGLYKTDLSASRHWQELVQQLTHDRAVWYDQSSYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLKDRRKLDDSIKAPLAFLFEDKTHSSSSIVKDKATSEPIRFTRRCVSVAPSRETAGELLLGKSGMYFVEENVPETHDNQSPHGETEPASFSWTYEEIKEVHKRWWQLRDNAMEIFLTNGRTLLLAFDNTKFRDDVYHNILTSDLPNLLEYGNISALTHLWSSGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILKDYTNETLDLQEASIYRNLSKPIAVQSKEKEDRYVDNYRYLEEEYKKGKREDDPMPPVQPYHYGSHYSNSGTVLHFLVRLPPFTKMFLAYQDQSFDIPDRTFHSMNTTWRLSSYESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNSERVNHVNLPPWARNDPRLFILIHRQALESDQVSQTLCQWIDLVFGLKQKGKAAVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFSASHISRAGTKLLSEAELPAAMGLLVQLAFRESREQPRDSGYPSPLPWIKGLKWGEYVGSPSAPDPVVCFSQPHGERFGSLLALPTRAICGLSRKFCLMMIYSKEQGVRSMHSTDIQWSAILSWGYTDNMLRLKSKQSEPPINFIQCSPLHQVSSCAWVPDSCQLFTGSKCGVITAYSNRFTTTMPTEMEVESQVDLYGHTAEVTGLFVCKPYSILISVSRDGTCILWDLNRLCYVQSLSGHKSPVNAVSASETTGDIATVCDSVGGGSDLRLWTINGDLIGHVHCREIICSVAFSNQPEGVSVNVIAGGLENGVVRLWSTWDLKPVREITFPKSNRPIISLTFSCDGHHLYTANSEGTVIAWCRRDQQRMKLPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	XM_017358862.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysosomal trafficking regulator (lyst), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358862.2

ORF Insert Sequence:

ATGAGTGGAG CCAGTAACAC CCTGGCACGG GAGTTCCTGA CGGATGTGCA CCAGCTGTGT 
AGTGCGGTGG CGCAGCGGGC GGAGGATGAG GAGGAGAGTC ACATGGTGGC CCTGGGAGAG 
TATCTGGTGC GCGGGCGTGG ATTCCTGCTC CTCAGCACCC TGGAATCTGT CATTGACCAG 
GAGTTGACCT GTCGGGAAGA ACTGCTTACG CTCTTATTGT CTCTGCTACC TCTGGTCTGG 
AAGATTCCTG TTCAGGAGGA GAAAGCCCCA GATTTCACCC TGCCTTCACT ACTTGAGGTG 
TTTCTTTGCC AAGAGGGAAG GACCATCTCT CAAAAGGCGG GGCAGGATCC ATGTGATGTG 
CAGAACTCTA CGAAGCGTTT ATCTGGCTCC TGGAAGTCAC GTCGCTCACG TAGGACAGCT 
CAGCGGTACT CTGTAAGGGA TGCACGCAAA TCTCAGCTTT CCACGTCAGA CTCTGAAGCC 
AACTCGGACG ATAAAACCAC AAACACTGGG AGCAAACATC GCAGATTCCA TAGTACGGCC 
ATTCATGTTA GTTGCCAGCT TCACCAGTCC AACAACTCCT CGCAACCAGC ACCGCAACAT 
CCCACTCTTG ATGCAATGGG CACTTTTGAG CCATCACAAC CTCACCTGAG ACTTTTTGGA 
TCTCACACAA TGGATCCTAA CATGCTATCA GACCCCACCA CTCTCTCCAT CTTCAACCGC 
ATGGAGAACT CGCCTTTTGA TCTCTGCCAT GTTCTTCTGT CCCTTCTTGA AAAGGTCTGC 
AAATTTGACA TGACCATCAA TCATAACCCA GGCTTGGCGG TCAGCGTTGT TCCCACCCTC 
ACAGAGATAC TCACTGACTT TGGTGACTGC TGTGGTCCCA GTGGTGGAGG GGGAGGTGGG 
ATTGATGAGT TAGCTGGAGG CTGGACTGAG GAGCCAATTG CACTTGTTCA AAGGATGCTT 
TTACGCACAA TTTTGCACTT GATGTTTGTT GATGTTGGTC AAAATGAGAC ACTGCCTGAT 
AATCTACGCC GAAGCTTGAC TGACCTCCTT AGAGCAACTT TAAAGATTCG TACCTGTTTA 
GAAAGACAAG CGGACCCTTT TGCTCCAAGG CCAAAGAAGA CTCTGCAGGA GATTCAAGAA 
GATTTTTCCT TCTCAAGGTA CCGCCACCGG GCCTTGCTAC TGCCAGAACT CTTACAGGGT 
GTCTTACAGC TACTGCTGGG CTGCTTGCAG GCCTCTGCTC CAAACCCTTT CTTTTTCAGT 
CAGTCGCTTG AGTTAGTTCA TGAATTTGTG CAACACCGTG GCTTGGAGCT CTTTGAGGTA 
ACAGCTCTAC GCCTTGAGGC CTTAGCAGAA GCTCACAATT CAGAGATTAG CAATGAAGCA 
TCAGAGCATC TGCGTTGTCT GATAGCTGAT GTGCTTAAAA TAATAAGTGC GGTCAAGAGG 
GCCAAGTCAG AGCAGCTACA CCAGTCTGTT TGTGCACGTC GTAGACACCG TCGCTGCGAA 
TACTCACACT TCTTACACCA TCATCGTGAC CTCTCAGGGC TGCCGGTATC TGCTTTCAAG 
CAGGCAGCTC GACGAAATCC CTTTGAGGAG GACTCCAAGG GTAATGATGG AGAAGTGCGG 
TACCCTGAAC GATGTTGTTG CCTTGCGGCT TGCGCTCACC AGTGTTTGCG TCTTTTTCAA 
CGCCTCTCGC CCTCAGGTCC TGCTGTGCTT CAGGTGCTTG CAGGAGTGCA AGCTGTTGGC 
ATCTGTTGCT GCATGGATCC AAGATCGGTA ATTGCACCAA TGCTCCAAGC ATTTCAGGCT 
CCAGGATTGC GGAGCTACCA GTCCCATGTG TTGAGTGTTC TTAGTAGACT TGTATTAGAA 
CAGCTTGGAG GAGGTCAACC CTCGGAAAAG GCCAAGCTTG CCTCATGCAA TATTTGCACA 
TTGGATAGCA GCCAGTTACC TGGTCTTGAA GAGACTCTGC AGGGATGTGG ACCTAAGAAA 
GAATTAAACT TGTCTACATC CCCATCACCA TCCTATCGTT CCCAAGGTAT TCTTCCCAGC 
GGAGGAGCTG AAGACATCCT ATGGAAGTGG GATGCTTTGG AAGCTTATCA GGAACTTGTT 
TTTGGGGAGG ACTGGCATTT GAGTCAACAG ATTGCCAGTC ATGTATGCCA GCTGACTTTG 
CGTGGCAATG CAGTAGTGCA GTGGCAACTC TACACTCATA TTTTTAACCC TGTCCTTCAG 
CGAGGTGTGG AATTAACCCA TCATGCACAG CAGCTTGGTG TGTCAACTAC CTGTAGCCAG 
GTCTGCAGTT ACCACACACA TTGTCTCCCA GTTGAGGTAC TGCTGGTTTA TCTACAAACT 
CTGCCTGCTC TTCTGAAGTC AAGCCGGGTA GTACGTGACC TGTTTCTGAG TTGTAATGGA 
CTGAACCAAG TCACCGAGTT GATCTACTTG GATTGCACTC GTCCATGGGC GCTAAAAGTG 
TTCGAGACAC TTATTTTAAA TGCATGGCGA AATCACACCG AATCTGCCGT GCATGAGCTA 
GAGAGAGCCG AAGCTCAGGA AAGGGAAGCT GTTCTTGGAC TCGTGGAGGA ACTAAGTACA 
CGTGGAACTG GTGATGCCCC CCAGAGCCTC ACCAAGTTTT ACGAAGGCCT GAAAGAGGCA 
TGTCCTCCTC CAAAGGGCAA AAGTGAAACA AGCGAGTTTT CTGGAGTAGG ACGTAATCGA 
GGCGAAGCAC ATTTGAACAC CATCAACCTT TTCCTGTGCG TTGCTTTCCT GTGTGTGAGC 
AAGGAAGCAG ACTCCGACAG AGACTCGGCT AATGACTCTG AAGACACCTC AGGTTATGAC 
AGTACAGCTA GTGAACCTTT AGGCGGGCGC CTACCTTGCC TGTCTCCAGA TAGTGTGGCT 
CTTCCCTCTA AAGAGCAGGT CAGGAGAGCT GCTGATGTTT GGTCAGTGTG CCGTTGGGTC 
TACATGGCCA GTCCAGTGTT CCAGAGGCAG TTCTTTAGGC TGGGAGGTCT AGACGTCTGT 
TCACGTTTAA TGACAATGGT CATTCAGAAA CTCACATCTA AAAACAAAGA CTGTAAATTT 
AAGAAAAAGA GGGAGAGTAA AGGCAAAAGC AGTCCGTCTC GTGAAGACTC ACCTACTCGA 
TGTATTACAG GACACTTTGA CAATATGGTG ACAAATGCAG GTACAGCAGA AACCCAGCAT 
CCTGAACTAA GTGACACTGT CAGCCTCTCT CAGACAGAGA GCAAGTCAAG TGATCCAATG 
CACAGGCTTG AAGAGGAATG GCCTTTTCAG AGTATTCGAC TCCTGGAGGC ACTTTTGGCT 
ATCTGCCTCC ACAGTGCCAA TTCAGCCCTA CAGAGGCTTG AGCCAGATCT TTCCTTTCAG 
CTGCAGTCTG TAGAGGAGAC CCTGTGTGAG GTCAGGGATC AGCTCTCTCG CTCTGGAGTT 
GTCAATTCTG AGCTGGCCGT TCCTCTGTTT GATTCCTTAC TCCGTGTGGC TCTGGCAGAG 
ATGTTCTGCA GTCCTGAAGT CATTGAGGAG AAACCTGATA AGAAGTGTGC GGTCAGTGAA 
GCGACTCCTC CAGCCGGTGA TCTGTCTGAG GATGTGGATG AGGCTCATGT GAGTGCAGTT 
CAGCCTCTGG GAGAAGAGGA AGGCTATGAC GCAGACAGCG AGAGTAACCC TGAGGACTCC 
ACCCGGCAGG AGGAAGGGCT AAAGGTGGAG GCTGGAGTCT CATTGAGGGA ATGTACATTG 
GGGATGGCAG GAGTGCCTCA GGGCTCCGTC CGCGGACGTC TGCTGTTTCC GGAGATCTGC 
CTGATGGAGC TGCAGCTGTT GGCCAGCGGT TCTCCAGACC TGGAGGTTTT GGGTCATGTG 
TTGCAGAGCC TGTTAGCCGC TGTGAAAGGC CACCACAACA ATGCAGCGCA GCTCTACCAG 
CAGGGTGGCG TGAAAGTCAT TCTTACTGGA TTCCCGAACA TTCTCAGCCA GTCAGATGCC 
TCATATAAAG AGTGCCAGAC AGTACTGATG GAGCTGCTTG TTGCCATGGT GAGTGACAGG 
ATCACAGCAG AGGAACTTGC CCTGTTGACC CGCCTCTTCC TGGAGAAGAC TCCGCCCACT 
GAGATCTTGC TACGAGGCGT GCTTCAGATC GTGGAGGCAA ACACAGACAC AGAACCGCTA 
TACTTCATGT CCTTTCCCAT GATCCCTGGC TCTGGGGGTC CTGGCCTGAG CCCCTCCCCT 
GGGACAAGGC CTCATGGGGC GACTGCTGGT AAGGGTGGTG TAAACACTAT TCTGAGGGGT 
AAACTACCGC TCGGCCGTGG TGAAGCAGAC ATGAGCCGAC CTGGTCACCT GCGCTCCTCG 
CCCTGGCATG CTGCACCCTT CCACCTGCCC TTGGTGGGAC AGAACTGCTG GCCTCATATG 
GCTAGTGGAT TCAGCGCATC GCTGTGGGTG CGGGCTGGGA AAGATGAAGA CGGACGATCT 
AAGAAGGAAG ATGAAGTGTG TGTGGCAGAG GAGTCTCCTC ACGGTTCTGC ACACGTCACA 
GAGCAGCAGA CAGAGCGCTG CCTCCTCCAT GTGCTCTCCA TGGGCTCTAA AGCACTGATG 
CTTCAGGTGT GGGTGAACAT CTCCACTAGA GCCTTCACCT TCAGGATCTG CATTGATCCC 
AATGATGAGA TGAAGGCGGG TCTTCTGGCG CAGGCGGAGT CTGGAGATGA AGTGATCCCA 
GCAGGCAGGT GGCAGCATTT AGCCCTGACC TACACACAGC AGCCCGAGGG CAAGAAGAAC 
ATCCATGGTC AGCTCAGCCT CTGGGTGTGT GGCATCAGGA AGTGTGATGT ATCTCTAGAC 
TTCACTCTGC CCAGGAAGTC CAGTTTGTCA TCAGATAGCA ACAAGACCTA CTGTATGCTT 
GGACACTATC AGCACTCCGC AGAAGAGCAG GCCAGCCCAA GTGCACGCTG GGATATGGGC 
ACACTGATGC TCTTCAATGG CTCTAGAATT GACACGACCG AGGCGTTTTA CCTGTACGCC 
AGCGGTCCTG ACCTCACCTC CATCATGCCC TGCAAATACG GCAAGCCTCA TGGCACACTG 
TCCAAGTACG TGACGCAGGA GGGTCTTCAG TGTGAGCAGA TACGAGAGCT GCTCATGAGG 
AATGCAGACG TTGACATCTC GCCTCTTGTT GAGAGCTTAG CAGTAGTGTA CACCCCCAGC 
ACGCCGTCTC TCTACACCAT ATATGAGCCG GTGATCCGTC TGAAGGGTCA GGCTAAATCA 
GCAGTGGTTT CCCAGCGGCC CTTCAGTGCC AAAGAGGTCC AGAGTTCCAT CCTGGAGTCT 
CCGTTTCTCA GGACCATGAT GCCCTGCCAG GCTTTCGGCC TCCACAGCGC ACTGCACAAG 
ATCGGCGGCA CCGGCTCCGT TGTCTTCCTG TTTGCTCGGG CGGTGGAGTT GTGCGACTGT 
GAGAAAACCC AAGCTCTGGC GCTGCAGGTG CTTCTGTCTC TGGTTAAAAA CAATCAGCAT 
CGCACTCATG AGATGGAGTG TTATCACGGT TATTCCATGA TTCACCAGGT GCTTATCAAA 
CCCAAGTGCA TTGTGGGATA TCACATCCTG AAGACGCTGT TAGACGGCTG CTGTAGCGGC 
TCCATCCTGA CGATAGGAGA AGACGGACAG TTCCATCTGG ATGCTGAATC CACTGTGGTG 
GTGCAAGATC TCAGACTGCT TTCTGAAATA TTGCTGGACT GGAAGATATG GGCCAAAGCT 
CAGTGTGGTG TGTGGGAGAC GCTGCTCACA GCATTAGAGA TCCTCATCCG GGTTCGTCAT 
CCTCAGCAGA TGTTCAACAT TCGACAGCTT CTGAAAGCTC AGGTGGTTCA TCGCTTTCTG 
TTAGCCTGCC AGGTGCTTCA GGAGCATCGG GACGAGTACC TGACCTCCAT CCCACAGGAG 
GTCTGCCTCT CCTTCGTCAA AATCATCCAG GAGGTTTTGG GGTCTCCTCC AGATATGGAC 
CTGCTGCGGC TGGTTTATAA TTTCCTGCTA GCCGTTCACC CCCCGACCAA CACTTACGTG 
TGCCACACGC CGACCAGCTT TTACTTCTCT CTGCACATCG ATGGTAAGCT GTACCAGGAG 
AAGGTCCAGT CCATGATGTA CCTGCGACAC TCCAGCAGTG GAGGGAAATC CGCCTGCAGC 
TCAGTGGTCT CACTGTCTCC CACTGTCTTC ACTGAGATGC CTGCTGAAGT ACGCCATACG 
TCCTCTATAG AGCATGAAGA CGATTTGTCC CTGCGGCCCC GAAGCACTGG CCCATCACCA 
CACTCCTCTC CAGCCCTGAC GCCCCGTCTT ACCCATGGAC GTGTGACGGG AACCACAGAA 
CCTGAAGGAG GAGCATCTCT CAGTACCCAG CAGGCCCTGG GCAGCACAGA GACCCTGAAG 
CGGGCTGGAG ACGAGCAGCT CCTCAGCAGC TGCGAATCAG CAAAGACCAT TTGTGATTCT 
CCCATCGCAG GCGCTGATGA AGAGGGGAGC ATTATGGGAA ACCGAACACC TTCTATCAGT 
GTAGAGGAGG AGCAGGACGG GCCCGCGGGA AGCCCAGTGG AGTCAGAAGA GCATTTCGAC 
TGGACCAGCG AAGAAGCCCC ACGCCGACCA GACAGCCTGA AAGGAATCCA GTCTTTCCAG 
AGGAGCCACA GTAATCTGGC CAGTCTGGGT TTGGCTTTTC CTGCGCAGAA CGGCTCTCTG 
ACTATTGCAC GCTGGCCCAC CGTTGCTGAC CGCGCCGCTC CACCTGACGA CTGGGAGAGC 
TACACATACT CACCCGGCTA CGACCGACAC AGCAAAGCAG ACTCCAGTAA TGACAGGTCC 
AGTGCGGAGG ACTGTCTGGT GCTGATCTGC TGTGGTCTGT ATGATCTGCT GAGAGGAGTT 
CTGCTTCTGC TGCCGGACCT CCTGCTGGAG GAAGTGATGG ACAAACTCAT CCAGCCTGAC 
GCTCTTCTCG TTCTGGTCAA CCACCCGTCT CCCCTCATCC AGCAGGGCGT CATGAAGCTC 
CTGGACGCCT ACTTCAGCCG CGCTCAGAAA GAGCAGAAGG AGAAGTTCCT GAAGAACCAC 
GGCTTCTCAC TGCTGGCCAA TCAGCTGTAC CAGCACCAGG GTTCACAGGG GCTGCTGGAG 
TGCTTCCTGG AGATGCTGTT CGGACGACCG GTCGGTCTCG AGGAAGATCT GGATCTGGAG 
GATATGGAGA ACATCTCTCC ATTCAGGAAG CGCTGTATCA TCCCAGTGCT GGGTCTGCTG 
GAGAACTCTC TGTATGAGAA CTCACTGGTG CACAATGGCC TGTGTCTGCT GCTGCAGCTC 
CTCAACGCCT GCCCGAAGCT GGCCGACATC CTGCTGGACC ACGGCTTACT CTACGTGCTC 
TTCAACACAC TCTCCACCCT CAACGGCCTG GAGAACGGGA TTCCTCCGAA TGACTACAGG 
CTGGTGGTGT GTGACATTCA GCAGCTGCTG GTGGCCGTCA CCATACACTC CTGCAGCTCT 
TCTGGATCTC AGTACTTCCG CATCATTGAA GACTTGATCA CACTGTTGGG CTACATGCAA 
ACCAGCAAAG TTCGCCGCAC ACAGGAAATG GCCCTGGCCT TGCAGTTTCG CGTGATTCAA 
GCAGCGATTG ATTTCATTAA GACCACAGCC AATCAGGACC CTCAGAAGTC CGGCAGTTAT 
GTGCATGTGC CCACATCACC ACACCACGCT CTGCAGCAGA AACGCAAAAG CATCGCAGGT 
TCAATGGGGC TGAAAGACTC CATAATCCCC CGTATCCCCC GGCAGCACTA CTGCTCGTAC 
GGCCTGATCC CTCTGCCCTC TCCGTTCAGC TTCCTGTCCC AGGATTCCCC TCTCCCTTCC 
CCTACCGGGT CAAACTCCTA CACGTTCCCC TCAGACGCAG GTCGTAGACG CTTCACACTG 
GCCCAGTCGG ACTCTCTGCT GACCCGCATG CGTTCTGTAG CCAGTGATGA GCTGACACAG 
ATGATGCAGC GCAGGATGAG TCAAGAAAAC CCCATCAGAG CCAGCGAGAC CGAGCTCATC 
CAGAGAATGC AGAGACTGGT GGTCCTGGCT GTCAACAGAC TCATTTATCA AGACATCAGC 
CATGATTTCT TTGACCTGCT GAACTTCCCT GAGTCTCCTG ATCACTCGAT GTCCATGCCA 
ACAGCAGGAG ATCAGACATC TGATGAGAAC GGCTCCACTC CGTCTACCCC GATCCCAGCC 
GGAAACATCA AGAGCTTCCG GAAGGACATT CTCAGACTGA TGATGGAGGG CATAAAGATC 
GGCCTGGGCA GCACTGGTCG TGGCGGCGCT CCTCGTCAGC AGTGGCGGCG AATCCTCTGG 
TCCTGCCGAG ACACATTCAG AGTGCAGATC GGCCGCATGC TGGTGCACAC GCTCAGTCCT 
GAACTTCCTC TGGAGGACAG GAAGGAGGCG CTAGAGTTCG TCCATGAGCA GAGCTACTCC 
GAGATCCTCA GAGAAAGCCT CAGCCCCGGC CTCGAGCATG GGCCCAAGCT GGCTCTGTAT 
CTGTACGAGC TGCTGCACGA TCACAAAGAC GCCCTGAGTA GAGAAGAGCA GGCGGCAGGA 
AACACCTTCA TGACGGCTCT GAAACTCTGC GGACACCGCT GCATCCCACC CAGCGCTCCA 
GCCAAACCCG ACCTCCTGAA AGCCATCAAA GAGGAGCAAC TAAAGTATGA ATCAGAGGCA 
AGAACCAGCA AAGGAGCCTG GGAAAAGAAG CTGTCCAACA CACAGAGGAG CCTGTTGCAG 
AGGCTGGATG GCAAGTCGAA GGACATCTCG AAGATCGCGG CGGACATCAC TCAGAACGTG 
TCTCTCAGGC AGGGCATGGA GAGGAAGAAG GTCATGCAGC ACATTAGAGG CCTGTATAAG 
ACTGACCTGA GCGCTAGTCG ACACTGGCAG GAGCTCGTCC AGCAGCTCAC ACACGACCGG 
GCGGTGTGGT ACGATCAGTC CTCTTATCCC ACTTCCTGGC AGCTGGACCC CACCGAGGGC 
CCGAACCGAG AGCGGAGACG CCTCCAGCGC TGCTACCTGA CCATCCCCAA CAAATATCTG 
CTCAAAGACC GTCGCAAACT AGATGATTCC ATTAAGGCTC CTCTGGCCTT CCTGTTTGAG 
GACAAGACTC ACTCCTCTTC ATCTATAGTG AAGGATAAAG CCACCAGTGA GCCCATCAGG 
TTCACAAGAA GGTGCGTAAG TGTGGCTCCC TCCAGAGAGA CAGCAGGAGA GCTCTTACTG 
GGAAAATCTG GGATGTATTT TGTGGAGGAA AATGTTCCTG AGACTCACGA TAACCAGAGT 
CCTCATGGAG AGACGGAGCC GGCCTCTTTC TCCTGGACGT ATGAGGAGAT AAAGGAGGTT 
CACAAGCGCT GGTGGCAGCT CAGAGACAAC GCCATGGAGA TCTTCCTGAC CAATGGAAGG 
ACACTGCTGC TAGCCTTTGA CAACACAAAG TTTCGTGATG ACGTCTACCA CAACATCCTG 
ACCTCAGATC TGCCCAACCT GCTGGAGTAT GGAAACATCA GCGCCCTCAC GCACCTCTGG 
AGCTCAGGAC AGATCACCAA CTTTGAGTAC CTGACGCATC TCAACAAGCA CGCGGGACGC 
TCCTTCAATG ACCTCATGCA GTATCCCGTC TTCCCCTTCA TCCTCAAAGA TTACACCAAT 
GAGACGCTCG ACCTGCAGGA GGCCTCTATT TACAGGAACC TCAGCAAGCC CATTGCAGTG 
CAGTCTAAAG AGAAGGAAGA CCGTTATGTG GACAATTACA GGTATCTGGA GGAGGAGTAT 
AAGAAGGGCA AGAGAGAGGA CGACCCTATG CCCCCTGTCC AGCCCTATCA CTACGGCTCT 
CATTACTCCA ACAGTGGCAC CGTCCTGCAC TTCCTGGTCC GACTGCCTCC TTTTACCAAA 
ATGTTTCTGG CATATCAAGA CCAAAGCTTT GATATTCCTG ACCGAACCTT CCACTCCATG 
AACACCACGT GGCGTCTGTC GTCCTACGAG TCCATGACTG ATGTCAAAGA GCTCATCCCA 
GAGTTCTTCT ACCTGCCCGA GTTCCTGGTC AACAGAGAAG GTTTTGACTT TGGCGTGCGG 
CAGAACAGCG AGCGGGTCAA CCATGTGAAT CTGCCGCCGT GGGCCAGAAA TGACCCGCGA 
CTCTTCATCC TCATCCACAG ACAGGCTCTG GAGTCCGATC AAGTCTCGCA GACGCTCTGC 
CAGTGGATCG ACCTGGTGTT TGGACTCAAG CAGAAAGGCA AAGCTGCGGT CCAGGCCATC 
AACGTCTTTC ATCCTGCAAC GTACTTTGGT ATGGACGTGT CTGCAGTTGA AGACCCTGTG 
CAGCGGCGGG CTCTAGAAAC CATGATCAAG ACGTACGGAC AAACACCCAG GCAGCTGTTC 
AGCGCTTCTC ACATCAGCAG AGCCGGAACC AAACTGCTGT CAGAGGCTGA GCTGCCCGCC 
GCCATGGGTC TGCTGGTGCA GCTGGCCTTC AGAGAGAGCC GAGAACAGCC CCGGGACAGC 
GGCTACCCGA GTCCTCTGCC GTGGATAAAG GGCTTGAAAT GGGGCGAGTA TGTGGGTTCA 
CCCAGTGCTC CGGACCCTGT GGTCTGTTTC AGTCAACCTC ACGGCGAACG CTTCGGCTCC 
CTGCTGGCAC TGCCCACACG TGCCATCTGC GGCCTCTCAC GCAAGTTCTG CCTCATGATG 
ATCTACAGCA AAGAGCAAGG TGTGAGGAGC ATGCACAGCA CTGACATACA GTGGTCTGCT 
ATTCTGAGCT GGGGTTACAC CGACAACATG CTGCGTCTGA AGAGCAAACA GAGCGAACCA 
CCAATCAACT TCATCCAGTG CTCACCGCTG CACCAGGTGA GCAGTTGTGC CTGGGTGCCG 
GACAGTTGTC AGCTGTTTAC AGGCAGCAAG TGTGGAGTCA TTACCGCCTA CAGCAACCGC 
TTCACCACCA CCATGCCCAC AGAGATGGAG GTGGAGTCGC AGGTTGATCT GTACGGACAC 
ACAGCCGAGG TCACGGGTCT GTTCGTCTGT AAACCCTACA GTATCCTCAT CAGTGTGAGT 
CGGGACGGAA CCTGCATACT CTGGGATCTC AACAGGTTGT GTTACGTACA GAGTCTCAGC 
GGTCACAAGA GTCCAGTGAA CGCGGTGTCA GCCAGCGAGA CCACCGGAGA CATCGCCACC 
GTCTGCGACT CAGTGGGTGG AGGAAGTGAC CTGCGGCTGT GGACCATTAA TGGAGATCTG 
ATTGGTCACG TTCACTGTCG TGAGATCATC TGTTCAGTGG CTTTCTCCAA TCAGCCGGAG 
GGAGTTTCAG TCAATGTGAT CGCAGGCGGA CTGGAGAACG GGGTGGTCAG GTTGTGGAGC 
ACGTGGGACC TGAAGCCAGT CAGAGAGATC ACGTTCCCAA AGTCCAACAG ACCTATCATA 
AGTCTGACGT TCTCATGTGA CGGTCATCAT CTATACACGG CTAACAGTGA GGGCACGGTG 
ATCGCGTGGT GTCGGCGAGA CCAGCAGCGC ATGAAGCTGC CCATGTTTTA CTCTTTCCTC 
AGCAGTTACG CCGCAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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lyst cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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