abca2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	abca2
Entrez Gene ID	100006090
Full Name	ATP-binding cassette, sub-family A (ABC1), member 2
Synonyms	si:dkey-266h7.1
General protein information	Preferred Names ATP-binding cassette, sub-family A (ABC1), member 2 Names ATP-binding cassette sub-family A member 2 ATP-binding cassette, sub-family A (ABC1), member 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165231.3	XP_005165288.1	ATP-binding cassette sub-family A member 2 isoform X3
XM_005165230.4	XP_005165287.1	ATP-binding cassette sub-family A member 2 isoform X2
XM_005165230.3	XP_005165287.1	ATP-binding cassette sub-family A member 2 isoform X2
XM_005165229.3	XP_005165286.1	ATP-binding cassette sub-family A member 2 isoform X1
XM_005165229.4	XP_005165286.1	ATP-binding cassette sub-family A member 2 isoform X1
XM_005165231.4	XP_005165288.1	ATP-binding cassette sub-family A member 2 isoform X3

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	ABCA2	XP_001117819.1
Mus musculus (house mouse)	Abca2	NP_031405.2
Gallus gallus (chicken)	ABCA2	XP_003642316.2
Xenopus tropicalis (tropical clawed frog)	abca2	XP_002942020.2
Canis lupus familiaris (dog)	ABCA2	XP_005625489.1
Homo sapiens (human)	ABCA2	NP_001597.2
Rattus norvegicus (Norway rat)	Abca2	NP_077372.1
Danio rerio (zebrafish)	abca2	XP_005165286.1
Bos taurus (cattle)	ABCA2	XP_002691759.2

Gene Ontology

The following abca2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the abca2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10381	XM_005165229.4 Latest version!	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa10382	XM_005165230.4 Latest version!	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa10383	XM_005165231.4 Latest version!	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa10382	XM_005165230.3	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa10381	XM_005165229.3	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa10383	XM_005165231.3	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

abca2 ( XM_005165229.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165286.1 Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X1, mRNA.

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CloneID	ODa10381
Clone ID Related Accession (Same CDS sequence)	XM_005165229.3 , XM_005165229.4
Accession Version	XM_005165229.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ATP-binding cassette sub-family A member 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG TGTCCTTCTA TAGCGCGGCG 
CCGCTCACCT CGGCGGGCAT CATTCCCATC ATGCAGTCGT TGTGTCCGGA TGGTCAGCGG 
GATGAGTTTG GCTTCCTGCA GTATAAAAAC TCCACTGTGA CTCAATTGCT GGAAAGGATA 
AGTGAAGTTG TGGAGCAGAA TCACTTGTTT ACATCAGACA GGCCCAGTCT GGGAGAGGAG 
CTGGAGTCTC TGCAGCAGCA CCTCGAAAGT CTGAGCTCAG CACAAGGCCC TCTAGAGAGC 
CACTACAACA CCAGCCACGG TTTCACTGTG GGAAGCGTCC TCAGGAACCA GTCGATGTTC 
CAGCAGTTTC TGATGCGAAA TCTCTCCCTG CCGAACGACA CGTCCAGCCT GCTGCTCTCC 
TCCCCCGTCA ATCTAAAAGA GGTATATAAT CTCATCTTTG GGACGTATCC CAAAGCGGGA 
TCAAAGGGAG GAGAAAGAGA TGAAGGACAG CACCAACTCA ACAGTCCTGG AGAGAAGCTT 
GTACAGCTGG AGAAGCAACT ACTTGGAGGC TGGCACAGTG TAGACGGGGG TCTGATTCAG 
AAGGCACTGA GAGATCCCAC AAAAGCTGCT CACCGTCAAG CTCTTCTCAA AGTCTTCTCT 
CAGGCGTTGG GCATCGCTGG CGGTGTAGGA GGTCAAAAAT TCGATCCTCA AGCACTGCGT 
GAGATGGAGG GAGTTCTGTT GACTGGTGCA GTGCTTGAAA GCCTTACCTG TGGGGAAGGA 
GGGAACAGTG AGATTGGCAA AATCCTATTG GTCCCTGAGA AACAACAGGC GGTTTTACAG 
GCTTACCGCA CGACTGTTTG TGGTTTAGGG GCGGGGCAGA GGGCGGAGCT TTTCAGACAG 
TTGAGTGAAG AACTGAGAGA TCAGATTGAC ACACAGAGAG TCTTTGATCA GTTGCGTCTT 
GAGCAGTCTA ATATGTCTGG TCTGCTGGAT CAGTCCCGTC TCGGGGCTCT CCTGAAGAAC 
CTGGCTGATG TGGAGCGGCT GCTGAGGGAC GTGGATCTAC TGTCAGGTCT GGCCAGACTG 
CTGCCGAAAG GGGCCTGTGC GGGTCACCAG CCTCCTCCTC AGGCCAACAC CACCTCCTGG 
AGCTCCAACT CTACCACATG GGGTCCCAAT ACAACTGACA GTTCTGTGGA GGAAGGAGAA 
GGAGCTTCTG AGAGCGAGGC AGAGGCTGAA AACCCCAAAT CACAGTTTTC TGCATTTGTT 
CAGCTGTGGG CAGGACTTCA ACCCATACTT TGTGGGAATA ATAGGATTTT CGAACCAGAA 
GCGCTGAAAC AAGGAAATAT GAGTTCTCTG GGCTTCACCA GCAAAGAACA GAGAAATTTA 
GGATTACTTG TTCATCTCAT GACTACAAAT CCCAAAATCC TCTACTCTCC CATTGACTCA 
GAGGTGGACA AGGTCATACA AAAGGCCAAT GAGACCTTTG CTTTTGTGGG GAATGTGACT 
CACTATGCCC GTGTGTGGCT CAACATCTCT GCTGAATTAA GGGCCTTTTT AGAGGAGGGT 
AAATTGCAAA ATCACCTGAT GTGGCTCCAG CAGTTTATTA CCGACCTGAA GAGGCACCAT 
GAGCTGCTGA ACGTATCAGA CAGTGAGCTG ATGAACAGTC TGCTGGAGGA GAACTTCACC 
CTGCCAAACA CAACCACCTT ACTGGAGCAG CTGCACACCA TTGATAATGC TGCTTGTGGA 
TGGACTAACT TCATGTCAAA GGTCAGTGTG GATATCTTCA GAGGCTTCCC TGATGAAGAA 
AGCATTGTCA ATTACACACT GAACCAGGCC TACCACGACA ACGTCTCAGT CTTTGCAAGT 
GTGATTTTCC AGACCAATAA AGATGGATCC CTGCCACCCC ATGTTATGTA TAAAATTCGA 
CAGAACTCGA GCTTTACCGA GAAAACCAAT GAAATCCGAC GTGCTTATTG GCGCCCAGGG 
CCAAACACAG GCGGCAAATT CTACTTCCTT TATGGCTTTG TGTGGATCCA AGATATGATT 
GAACGGGCAA TCATCAACAC CTTTGTTGGC CACGATGTTG TGGAGCCTGG AAATTACGTG 
CAGATGTTTC CTTATCCGTG CTACACCAGA GACGATTTTC TCTTTGTTAT CGAGCACATG 
ATGCCTCTGT GTATGGTCAT ATCCTGGGTC TACTCTGTGG CCATGATGAT CCAGCACATC 
GTAGCAGAGA AGGAGCAAAG ACTGAAAGAG GTAATGAAGA TGATGGGCTT GAATAACGCA 
GTGCACTGGG TGGCCTGGTT CATCACAGGC TTCGTGCAGC TTTCCATCTC AGTGACGGCC 
CTCACTGCCA TACTGAAATA CGGGAAAGTC CTCCTTCACA GCGATCCCTT CATCATATGG 
CTTTTTCTTA CAATCTACGC TGTCGCAACC ATCATGTTCT GTTTCCTGGT GTCGGTGTTG 
TACTCTAAAG CCAAACTGGC CTCGGCATGT GGAGGAATCA TCTACTTCCT GAGTTATGTG 
CCCTACATGT ATGTGGCCAT AAGAGAAGAG GTGGCTCACG ATAAGATCAC TGCCTTTGAG 
AAGTGCATAG CGTCTTTGAT GTCTACCACA GCGTTCGGTC TGGGATCCAA GTACTTTGCC 
CTGTATGAAG TGGCTGGCGT GGGAATTCAG TGGCGCACTA TTAACCAGTC ACCTGTAGAG 
GGCGATGACT TCAATCTACT GCTGTCTATG GTCATGCTCA CTATTGACGC CATTGTATAT 
GGTGTGTTGA CCTGGTATAT AGAAGCCGTC CATCCAGGGA TGTATGGACT GCCTCGGCCA 
TGGTATTTCC CCTTGCAGAA GTCCTATTGG TTAGGTAGTG GCCGCATAGA GACGTGGGAG 
TGGCCGTGGG GTGGAGGCAC CAGGCTGAGT GTTATGGAAG AGGACCAGGC TTGTGCCATG 
GAGCATAGAC GCTCAGAGGA AACCCGTGGA ATTGAAGAAG AGCCCAATCA TTTGCCGCTG 
GTGGTGTGCA TCGACAAACT GACCAAAGTC TACAAGACGG GCAGCAAACT GGCTCTAAAC 
AAGCTGAGCC TGAACCTGCA TGAGAACCAG GTTGTCTCTT TCTTGGGACA CAATGGGGCT 
GGCAAGACCA CCACTATGTC CATTCTCACG GGACTCTTTC CACCCACCTC TGGCTCTGCC 
ACCATATACG GCCATGACAT CCGCACAGAG ATGGAGCGCA TCAGGCAGAA CTTGGGCATG 
TGTCCCCAGC ACAACGTCCT GTTTGATAAG CTCAGTGTGG AGGAACATCT GTGGTTCTAC 
TCACGGCTGA AAGGCATGGC AGAGGAAGAT ATTCGCAAGG AGATGGACAA GATGATAGAG 
GATCTCGAAT TATCTAATAA ACGTCACAGT CTGGTTCAAA CCCTGTCAGG AGGGATGAAA 
AGGAAGCTGT CAGTGGCCAT TGCTTTTGTA GGTGGGTCTC GGGCAGTGAT TTTGGATGAA 
CCAACAGCAG GAGTAGACCC ATATGCCCGT CGAGCGATCT GGGACCTCAT CCTGAAATAT 
AAGCAAGGTC GCACCATCCT GCTGTCCACT CACCACATGG ATGAGGCTGA TCTGCTGGGT 
GACCGCATTG CCATCATCTC TCATGGCAAG CTCAAGTGCT GCGGTTCTCC TCTGTTCCTC 
AAGAGCACAT ATGGAGACGG ATACAAACTC ACACTTGTTA AAAAACAGAG TGACTCACAC 
ACTGCAGATC AGTCAAGCCA GTCTCCGTCC TCTTCGATAT CTCCATGTTC AGAAAACAGA 
GTAACTCAGT TCATCCGTCA GTATGTTGCT TCCTGCCTGC TGGTGTCCGA CTCCAACACT 
GAACTCTCTT ATGTCCTACC ATCAGAGGCT GTCAAGAAAG GCTGCTTTGA GAGACTCTTC 
CAGGCTCTGG AGCAGAGTTT AGACAGCTTG GCCTTGACCA GCTTTGGCGT GATGGACACC 
ACATTGGAGG AGGTGTTTCT AAAGGTGTCA GAAGAAGATC TGTCGCTGGA AAACAGCGAT 
GCAGACATGA AGGACTCTCC AGGCGGAGCA TCAGCCGGGA AGCCGTCAGG TCTGTTAGGA 
GGGCCTCAGT GTGAAGGAGC TCCAGCCGCT GCTGTGATGA GGCCAGAGGT GGAGCTCAGC 
AACCTGATGA TGTGCTCCAG GCTGAGCCCA AGCCAAGCAT CCCTGCAGTC CGGCTCTTCT 
CTGGGCTCAG TGAGAGGAGA TGAAGGGGGT CCCTATTCTG AGTTCTATGG AGACTATTGC 
CCACTGTTTG ACAACGGTCA GGATCTGGAC TCTGCCAGTC TCAGAGATGA AGAGTCATCT 
CCTCAGCCCA CTGTACTCGA GGGCCAGGGC AGCTTCAAGC TCGATGGCTG GTGGCTTAAA 
CTCCGCCAGT TTCATGGGCT GATCGTGAAA AGGTTTCACT GTGCCAAGAG GAACACCAAG 
GGCATTTTCT CTCAGATTCT GCTGCCTGCT TTCTTCGTAT GTGTGGCAAT GACTGTGGCT 
CTGTCTGTGC CTGAGATTGG TGACCTTCCT CCCCTGATCC TGTCTCCATC TCAATATCAC 
AACTACACAC AGCCTCGAGG AAACTTCATC CCCTACGCCA ATGAAGACAG ATCTCAATAC 
AGGACTAAGT TTTCTCCAGA CGCAGGCCCA CAGAGGATTG TTAACACCCT CCGTCTGCCA 
TCTGGAGTCG GTGCGACTTG TATCCTGAAG ACTCCCTTTA ACAGCACCCT GGACCAACTG 
GCCCAAACTC TCAATCCTTA TGCCAACAAC TCTAAAACCC TGGCAGCCCG CTACTTTGAC 
TCTATGTGCC TGGACTCCTT CACGCAGGGT GTGCCTTTGT CGAATTTCGT TCCCCCTCCG 
CCCTCTCCTG CCCCCTCAGA CGACCCGGAG GCTCATTTTG AAGACGGCCT GTGGAACTAC 
ACTGCAGCTC CTACTACTGT CAGAGAGATG GCCACGTCTC CCCCGACTCT GCCCTTGAGT 
ATCCATGAGC CGGTGCGCTG TATCTGCTCT ATGCAGGGCA CAGGCTTCTC CTGCCCCAGT 
GGAGTTGGGG GTCGACCTCC TCTGATGAAA GTGGTGACCG GAGACATCCT GGTGGACATT 
ACAGGTCGCA ACGTGTCTGA ATATCTCCTG TACACGTCTG ATCGGCTCAG GTTACACAGA 
TATGGAGGCT TTACTGTTGG AAACATTCAG AAATCTGTCC CAGCATCCTT CGGGAGAAAA 
ACACCACCTA TGGTGCGCAA AATAGCAGTG AGGCGATCCT CACAGGTGCT GTATAATAAC 
AAAGGCTATC ACAGTATGCC AACATATCTA AATGTCCTCA ACAATGCCAT TCTCAGAGCC 
AATCTGCCCA GTAGTAAAGG AAACCCTTCT GCATACGGCA TTACAGTGAC CAATCATCCC 
ATGAACAGAA CCAGTGCCAG TCTTTCTCTG GATTATCTGC TCCAGGGCAC TGATGTGGTT 
ATTGCCATCT TCATCATTGT AGCCATGTCT TTCGTGCCTG CGAGCTTTGT GGTCTTTCTG 
GTAGCGGAAA AATCCACCAA AGCCAAACAC CTGCAGTTTG TCAGCGGATG CGACCCTGTC 
ACGTACTGGC TAGCAAACTA CATATGGGAC ATGCTGAACT ACCTGGTTCC AGCTACATGC 
TGTGTCCTAA TTTTGTTTGT GTTCGACCTT CCTGCGTACA CGTCACCTAC AAATTTCCCA 
GCAGTGCTCT CTCTGTTTCT GCTTTATGGC TGGTCTATAA CCCCCATCAT GTACCCAGCC 
TCCTTCTGGT TTGAGGTGCC CAGCACAGCT TATGTTTTCC TCATAGTCAT CAATCTCTTC 
ATTGGCATCA CAGCCACGGT GGCCACTTTC CTCCTCCAGC TGTTTGAGCG CGACAAGGAC 
CTGAAGTTGG TAAACAGCTA CCTGAAGTCA TGTTTTCTCA TCTTCCCCAA CTATAACCTG 
GGTCACGGAT TGATGGAGAT GGCCTATAAT GAGTATATCA ATGAATATTA CGCCAAGATA 
GGCCAGTTTG ATAAAGTGAA GTCTCCATTT GAATGGGACA TTGTGACGCG GGGACTGGTA 
GCAATGACCA TTGAGGGCTT CGTCGGCTTT CTCATTACAA TTCTGTGCCA GTACAACTTC 
CTCAGGAAAG CCCAGAGAGT GCCTGTCAAC AGTCAGCCAG TGGAGGACGA TGATGTTGAT 
GTGGCCTGTG AAAGACGCCG AGTTCTGAGG GGAGACGCTG ACAACGACAT GCTGAAGATC 
GACAACCTAA CCAAGGTGTA CAAGTCTCGT AAGATGGGCC GGATCCTGGC AGTGGACCGA 
TTGTGTCTGG GGGTCCGTCC TGGTGAATGC TTCGGTCTGC TGGGAGTGAA TGGGGCAGGA 
AAGACCACCA CCTTCAAAAT GTTGACAGGA GATGAGAGCA CAACAGGGGG AGAAGCCTTT 
ATCCAGGAAC ACAGTATCTT GCGGGAGCTG TTACGTGTGC AGCAGAGCAT CGGTTACTGC 
CCTCAGTTTG ACGCTCTGTT TGATGATCTG ACGGCTCGAG AGCATCTGGA GCTCTACACG 
CGCCTCCGCG GCATCCCATG GAAAGATGAG GAGCGGGTGG TTCAGTGGGC TTTGGAGAAG 
TTGGAGTTGT CCAAGTATGC CGACAAGCCT GCTGGCACCT ACAGCGGAGG CAACAAACGC 
AAACTCTCTA CAGCCATTGC TCTGATTGGA TACCCATCGC TCATCTTTTT GGATGAGCCC 
ACCACAGGTA TGGACCCAAA GGCTCGCCGT TTCTTATGGA ACCTAATTCT TGACATCATC 
AAGACGGGCC GCTCAGTAGT GTTGACGTCA CACAGTATGG AGGAGTGTGA AGCGCTGTGC 
ACTAGACTGG GCATCATGGT TAATGGCAGG TTCAAGTGTC TAGGAAGCAT CCAGCATCTC 
AAAAACAGAT TCGGAGATGG ATACATGATA ACAGTACGCA CTAAGACCAC CGCTAGCGTT 
AAAGAAGTGA TTCGGTTTTT TAACAGAAAT TTTCCTGAGG CAATTCTAAA GGAGCGACAC 
CACACAAAGA TTCAGTACCA GCTGAAGTCA GAGAACATCT CTCTGGCTCA GGTCTTCAGC 
AAAATGGAGC AGGTGGTGGA GGTGCTGAGC ATCGAGGATT ATTCCGTCAG CCAAACCACA 
CTGGATAACG TCTTTGTTAA CTTTGCAAAA AAGCAAAGCG ACAACTTAGA GCAGCAAGAA 
AGTTCTCCAT CTAGTGGTGG CCAGTCGCCA CTGCAGCATC TGCTCAGCCT CCTCAGGCCT 
CGTCCTGCAA ACACTGAGCT CAACGCCCTC GTGAGTGAAG AGCCTGAGGA ACTGGAGAGT 
GATGATGACG AAGGACTGAT CAGCTTTGAG GAAGAGAGGG TGCAGTTGTC CTTTAATACA 
GACACTCTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165286.1
CDS	552..8066
Translation	MGFLHQLHLLLWKNVTLKRRGPWVLAFEIFIPLVLFFILLGLRQKKPAIPVKEVSFYSAAPLTSAGIIPIMQSLCPDGQRDEFGFLQYKNSTVTQLLERISEVVEQNHLFTSDRPSLGEELESLQQHLESLSSAQGPLESHYNTSHGFTVGSVLRNQSMFQQFLMRNLSLPNDTSSLLLSSPVNLKEVYNLIFGTYPKAGSKGGERDEGQHQLNSPGEKLVQLEKQLLGGWHSVDGGLIQKALRDPTKAAHRQALLKVFSQALGIAGGVGGQKFDPQALREMEGVLLTGAVLESLTCGEGGNSEIGKILLVPEKQQAVLQAYRTTVCGLGAGQRAELFRQLSEELRDQIDTQRVFDQLRLEQSNMSGLLDQSRLGALLKNLADVERLLRDVDLLSGLARLLPKGACAGHQPPPQANTTSWSSNSTTWGPNTTDSSVEEGEGASESEAEAENPKSQFSAFVQLWAGLQPILCGNNRIFEPEALKQGNMSSLGFTSKEQRNLGLLVHLMTTNPKILYSPIDSEVDKVIQKANETFAFVGNVTHYARVWLNISAELRAFLEEGKLQNHLMWLQQFITDLKRHHELLNVSDSELMNSLLEENFTLPNTTTLLEQLHTIDNAACGWTNFMSKVSVDIFRGFPDEESIVNYTLNQAYHDNVSVFASVIFQTNKDGSLPPHVMYKIRQNSSFTEKTNEIRRAYWRPGPNTGGKFYFLYGFVWIQDMIERAIINTFVGHDVVEPGNYVQMFPYPCYTRDDFLFVIEHMMPLCMVISWVYSVAMMIQHIVAEKEQRLKEVMKMMGLNNAVHWVAWFITGFVQLSISVTALTAILKYGKVLLHSDPFIIWLFLTIYAVATIMFCFLVSVLYSKAKLASACGGIIYFLSYVPYMYVAIREEVAHDKITAFEKCIASLMSTTAFGLGSKYFALYEVAGVGIQWRTINQSPVEGDDFNLLLSMVMLTIDAIVYGVLTWYIEAVHPGMYGLPRPWYFPLQKSYWLGSGRIETWEWPWGGGTRLSVMEEDQACAMEHRRSEETRGIEEEPNHLPLVVCIDKLTKVYKTGSKLALNKLSLNLHENQVVSFLGHNGAGKTTTMSILTGLFPPTSGSATIYGHDIRTEMERIRQNLGMCPQHNVLFDKLSVEEHLWFYSRLKGMAEEDIRKEMDKMIEDLELSNKRHSLVQTLSGGMKRKLSVAIAFVGGSRAVILDEPTAGVDPYARRAIWDLILKYKQGRTILLSTHHMDEADLLGDRIAIISHGKLKCCGSPLFLKSTYGDGYKLTLVKKQSDSHTADQSSQSPSSSISPCSENRVTQFIRQYVASCLLVSDSNTELSYVLPSEAVKKGCFERLFQALEQSLDSLALTSFGVMDTTLEEVFLKVSEEDLSLENSDADMKDSPGGASAGKPSGLLGGPQCEGAPAAAVMRPEVELSNLMMCSRLSPSQASLQSGSSLGSVRGDEGGPYSEFYGDYCPLFDNGQDLDSASLRDEESSPQPTVLEGQGSFKLDGWWLKLRQFHGLIVKRFHCAKRNTKGIFSQILLPAFFVCVAMTVALSVPEIGDLPPLILSPSQYHNYTQPRGNFIPYANEDRSQYRTKFSPDAGPQRIVNTLRLPSGVGATCILKTPFNSTLDQLAQTLNPYANNSKTLAARYFDSMCLDSFTQGVPLSNFVPPPPSPAPSDDPEAHFEDGLWNYTAAPTTVREMATSPPTLPLSIHEPVRCICSMQGTGFSCPSGVGGRPPLMKVVTGDILVDITGRNVSEYLLYTSDRLRLHRYGGFTVGNIQKSVPASFGRKTPPMVRKIAVRRSSQVLYNNKGYHSMPTYLNVLNNAILRANLPSSKGNPSAYGITVTNHPMNRTSASLSLDYLLQGTDVVIAIFIIVAMSFVPASFVVFLVAEKSTKAKHLQFVSGCDPVTYWLANYIWDMLNYLVPATCCVLILFVFDLPAYTSPTNFPAVLSLFLLYGWSITPIMYPASFWFEVPSTAYVFLIVINLFIGITATVATFLLQLFERDKDLKLVNSYLKSCFLIFPNYNLGHGLMEMAYNEYINEYYAKIGQFDKVKSPFEWDIVTRGLVAMTIEGFVGFLITILCQYNFLRKAQRVPVNSQPVEDDDVDVACERRRVLRGDADNDMLKIDNLTKVYKSRKMGRILAVDRLCLGVRPGECFGLLGVNGAGKTTTFKMLTGDESTTGGEAFIQEHSILRELLRVQQSIGYCPQFDALFDDLTAREHLELYTRLRGIPWKDEERVVQWALEKLELSKYADKPAGTYSGGNKRKLSTAIALIGYPSLIFLDEPTTGMDPKARRFLWNLILDIIKTGRSVVLTSHSMEECEALCTRLGIMVNGRFKCLGSIQHLKNRFGDGYMITVRTKTTASVKEVIRFFNRNFPEAILKERHHTKIQYQLKSENISLAQVFSKMEQVVEVLSIEDYSVSQTTLDNVFVNFAKKQSDNLEQQESSPSSGGQSPLQHLLSLLRPRPANTELNALVSEEPEELESDDDEGLISFEEERVQLSFNTDTLC

Target ORF information:

RefSeq Version	XM_005165229.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165229.3

ORF Insert Sequence:

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG TGTCCTTCTA TAGCGCGGCG 
CCGCTCACCT CGGCGGGCAT CATTCCCATC ATGCAGTCGT TGTGTCCGGA TGGTCAGCGG 
GATGAGTTTG GCTTCCTGCA GTATAAAAAC TCCACTGTGA CTCAATTGCT GGAAAGGATA 
AGTGAAGTTG TGGAGCAGAA TCACTTGTTT ACATCAGACA GGCCCAGTCT GGGAGAGGAG 
CTGGAGTCTC TGCAGCAGCA CCTCGAAAGT CTGAGCTCAG CACAAGGCCC TCTAGAGAGC 
CACTACAACA CCAGCCACGG TTTCACTGTG GGAAGCGTCC TCAGGAACCA GTCGATGTTC 
CAGCAGTTTC TGATGCGAAA TCTCTCCCTG CCGAACGACA CGTCCAGCCT GCTGCTCTCC 
TCCCCCGTCA ATCTAAAAGA GGTATATAAT CTCATCTTTG GGACGTATCC CAAAGCGGGA 
TCAAAGGGAG GAGAAAGAGA TGAAGGACAG CACCAACTCA ACAGTCCTGG AGAGAAGCTT 
GTACAGCTGG AGAAGCAACT ACTTGGAGGC TGGCACAGTG TAGACGGGGG TCTGATTCAG 
AAGGCACTGA GAGATCCCAC AAAAGCTGCT CACCGTCAAG CTCTTCTCAA AGTCTTCTCT 
CAGGCGTTGG GCATCGCTGG CGGTGTAGGA GGTCAAAAAT TCGATCCTCA AGCACTGCGT 
GAGATGGAGG GAGTTCTGTT GACTGGTGCA GTGCTTGAAA GCCTTACCTG TGGGGAAGGA 
GGGAACAGTG AGATTGGCAA AATCCTATTG GTCCCTGAGA AACAACAGGC GGTTTTACAG 
GCTTACCGCA CGACTGTTTG TGGTTTAGGG GCGGGGCAGA GGGCGGAGCT TTTCAGACAG 
TTGAGTGAAG AACTGAGAGA TCAGATTGAC ACACAGAGAG TCTTTGATCA GTTGCGTCTT 
GAGCAGTCTA ATATGTCTGG TCTGCTGGAT CAGTCCCGTC TCGGGGCTCT CCTGAAGAAC 
CTGGCTGATG TGGAGCGGCT GCTGAGGGAC GTGGATCTAC TGTCAGGTCT GGCCAGACTG 
CTGCCGAAAG GGGCCTGTGC GGGTCACCAG CCTCCTCCTC AGGCCAACAC CACCTCCTGG 
AGCTCCAACT CTACCACATG GGGTCCCAAT ACAACTGACA GTTCTGTGGA GGAAGGAGAA 
GGAGCTTCTG AGAGCGAGGC AGAGGCTGAA AACCCCAAAT CACAGTTTTC TGCATTTGTT 
CAGCTGTGGG CAGGACTTCA ACCCATACTT TGTGGGAATA ATAGGATTTT CGAACCAGAA 
GCGCTGAAAC AAGGAAATAT GAGTTCTCTG GGCTTCACCA GCAAAGAACA GAGAAATTTA 
GGATTACTTG TTCATCTCAT GACTACAAAT CCCAAAATCC TCTACTCTCC CATTGACTCA 
GAGGTGGACA AGGTCATACA AAAGGCCAAT GAGACCTTTG CTTTTGTGGG GAATGTGACT 
CACTATGCCC GTGTGTGGCT CAACATCTCT GCTGAATTAA GGGCCTTTTT AGAGGAGGGT 
AAATTGCAAA ATCACCTGAT GTGGCTCCAG CAGTTTATTA CCGACCTGAA GAGGCACCAT 
GAGCTGCTGA ACGTATCAGA CAGTGAGCTG ATGAACAGTC TGCTGGAGGA GAACTTCACC 
CTGCCAAACA CAACCACCTT ACTGGAGCAG CTGCACACCA TTGATAATGC TGCTTGTGGA 
TGGACTAACT TCATGTCAAA GGTCAGTGTG GATATCTTCA GAGGCTTCCC TGATGAAGAA 
AGCATTGTCA ATTACACACT GAACCAGGCC TACCACGACA ACGTCTCAGT CTTTGCAAGT 
GTGATTTTCC AGACCAATAA AGATGGATCC CTGCCACCCC ATGTTATGTA TAAAATTCGA 
CAGAACTCGA GCTTTACCGA GAAAACCAAT GAAATCCGAC GTGCTTATTG GCGCCCAGGG 
CCAAACACAG GCGGCAAATT CTACTTCCTT TATGGCTTTG TGTGGATCCA AGATATGATT 
GAACGGGCAA TCATCAACAC CTTTGTTGGC CACGATGTTG TGGAGCCTGG AAATTACGTG 
CAGATGTTTC CTTATCCGTG CTACACCAGA GACGATTTTC TCTTTGTTAT CGAGCACATG 
ATGCCTCTGT GTATGGTCAT ATCCTGGGTC TACTCTGTGG CCATGATGAT CCAGCACATC 
GTAGCAGAGA AGGAGCAAAG ACTGAAAGAG GTAATGAAGA TGATGGGCTT GAATAACGCA 
GTGCACTGGG TGGCCTGGTT CATCACAGGC TTCGTGCAGC TTTCCATCTC AGTGACGGCC 
CTCACTGCCA TACTGAAATA CGGGAAAGTC CTCCTTCACA GCGATCCCTT CATCATATGG 
CTTTTTCTTA CAATCTACGC TGTCGCAACC ATCATGTTCT GTTTCCTGGT GTCGGTGTTG 
TACTCTAAAG CCAAACTGGC CTCGGCATGT GGAGGAATCA TCTACTTCCT GAGTTATGTG 
CCCTACATGT ATGTGGCCAT AAGAGAAGAG GTGGCTCACG ATAAGATCAC TGCCTTTGAG 
AAGTGCATAG CGTCTTTGAT GTCTACCACA GCGTTCGGTC TGGGATCCAA GTACTTTGCC 
CTGTATGAAG TGGCTGGCGT GGGAATTCAG TGGCGCACTA TTAACCAGTC ACCTGTAGAG 
GGCGATGACT TCAATCTACT GCTGTCTATG GTCATGCTCA CTATTGACGC CATTGTATAT 
GGTGTGTTGA CCTGGTATAT AGAAGCCGTC CATCCAGGGA TGTATGGACT GCCTCGGCCA 
TGGTATTTCC CCTTGCAGAA GTCCTATTGG TTAGGTAGTG GCCGCATAGA GACGTGGGAG 
TGGCCGTGGG GTGGAGGCAC CAGGCTGAGT GTTATGGAAG AGGACCAGGC TTGTGCCATG 
GAGCATAGAC GCTCAGAGGA AACCCGTGGA ATTGAAGAAG AGCCCAATCA TTTGCCGCTG 
GTGGTGTGCA TCGACAAACT GACCAAAGTC TACAAGACGG GCAGCAAACT GGCTCTAAAC 
AAGCTGAGCC TGAACCTGCA TGAGAACCAG GTTGTCTCTT TCTTGGGACA CAATGGGGCT 
GGCAAGACCA CCACTATGTC CATTCTCACG GGACTCTTTC CACCCACCTC TGGCTCTGCC 
ACCATATACG GCCATGACAT CCGCACAGAG ATGGAGCGCA TCAGGCAGAA CTTGGGCATG 
TGTCCCCAGC ACAACGTCCT GTTTGATAAG CTCAGTGTGG AGGAACATCT GTGGTTCTAC 
TCACGGCTGA AAGGCATGGC AGAGGAAGAT ATTCGCAAGG AGATGGACAA GATGATAGAG 
GATCTCGAAT TATCTAATAA ACGTCACAGT CTGGTTCAAA CCCTGTCAGG AGGGATGAAA 
AGGAAGCTGT CAGTGGCCAT TGCTTTTGTA GGTGGGTCTC GGGCAGTGAT TTTGGATGAA 
CCAACAGCAG GAGTAGACCC ATATGCCCGT CGAGCGATCT GGGACCTCAT CCTGAAATAT 
AAGCAAGGTC GCACCATCCT GCTGTCCACT CACCACATGG ATGAGGCTGA TCTGCTGGGT 
GACCGCATTG CCATCATCTC TCATGGCAAG CTCAAGTGCT GCGGTTCTCC TCTGTTCCTC 
AAGAGCACAT ATGGAGACGG ATACAAACTC ACACTTGTTA AAAAACAGAG TGACTCACAC 
ACTGCAGATC AGTCAAGCCA GTCTCCGTCC TCTTCGATAT CTCCATGTTC AGAAAACAGA 
GTAACTCAGT TCATCCGTCA GTATGTTGCT TCCTGCCTGC TGGTGTCCGA CTCCAACACT 
GAACTCTCTT ATGTCCTACC ATCAGAGGCT GTCAAGAAAG GCTGCTTTGA GAGACTCTTC 
CAGGCTCTGG AGCAGAGTTT AGACAGCTTG GCCTTGACCA GCTTTGGCGT GATGGACACC 
ACATTGGAGG AGGTGTTTCT AAAGGTGTCA GAAGAAGATC TGTCGCTGGA AAACAGCGAT 
GCAGACATGA AGGACTCTCC AGGCGGAGCA TCAGCCGGGA AGCCGTCAGG TCTGTTAGGA 
GGGCCTCAGT GTGAAGGAGC TCCAGCCGCT GCTGTGATGA GGCCAGAGGT GGAGCTCAGC 
AACCTGATGA TGTGCTCCAG GCTGAGCCCA AGCCAAGCAT CCCTGCAGTC CGGCTCTTCT 
CTGGGCTCAG TGAGAGGAGA TGAAGGGGGT CCCTATTCTG AGTTCTATGG AGACTATTGC 
CCACTGTTTG ACAACGGTCA GGATCTGGAC TCTGCCAGTC TCAGAGATGA AGAGTCATCT 
CCTCAGCCCA CTGTACTCGA GGGCCAGGGC AGCTTCAAGC TCGATGGCTG GTGGCTTAAA 
CTCCGCCAGT TTCATGGGCT GATCGTGAAA AGGTTTCACT GTGCCAAGAG GAACACCAAG 
GGCATTTTCT CTCAGATTCT GCTGCCTGCT TTCTTCGTAT GTGTGGCAAT GACTGTGGCT 
CTGTCTGTGC CTGAGATTGG TGACCTTCCT CCCCTGATCC TGTCTCCATC TCAATATCAC 
AACTACACAC AGCCTCGAGG AAACTTCATC CCCTACGCCA ATGAAGACAG ATCTCAATAC 
AGGACTAAGT TTTCTCCAGA CGCAGGCCCA CAGAGGATTG TTAACACCCT CCGTCTGCCA 
TCTGGAGTCG GTGCGACTTG TATCCTGAAG ACTCCCTTTA ACAGCACCCT GGACCAACTG 
GCCCAAACTC TCAATCCTTA TGCCAACAAC TCTAAAACCC TGGCAGCCCG CTACTTTGAC 
TCTATGTGCC TGGACTCCTT CACGCAGGGT GTGCCTTTGT CGAATTTCGT TCCCCCTCCG 
CCCTCTCCTG CCCCCTCAGA CGACCCGGAG GCTCATTTTG AAGACGGCCT GTGGAACTAC 
ACTGCAGCTC CTACTACTGT CAGAGAGATG GCCACGTCTC CCCCGACTCT GCCCTTGAGT 
ATCCATGAGC CGGTGCGCTG TATCTGCTCT ATGCAGGGCA CAGGCTTCTC CTGCCCCAGT 
GGAGTTGGGG GTCGACCTCC TCTGATGAAA GTGGTGACCG GAGACATCCT GGTGGACATT 
ACAGGTCGCA ACGTGTCTGA ATATCTCCTG TACACGTCTG ATCGGCTCAG GTTACACAGA 
TATGGAGGCT TTACTGTTGG AAACATTCAG AAATCTGTCC CAGCATCCTT CGGGAGAAAA 
ACACCACCTA TGGTGCGCAA AATAGCAGTG AGGCGATCCT CACAGGTGCT GTATAATAAC 
AAAGGCTATC ACAGTATGCC AACATATCTA AATGTCCTCA ACAATGCCAT TCTCAGAGCC 
AATCTGCCCA GTAGTAAAGG AAACCCTTCT GCATACGGCA TTACAGTGAC CAATCATCCC 
ATGAACAGAA CCAGTGCCAG TCTTTCTCTG GATTATCTGC TCCAGGGCAC TGATGTGGTT 
ATTGCCATCT TCATCATTGT AGCCATGTCT TTCGTGCCTG CGAGCTTTGT GGTCTTTCTG 
GTAGCGGAAA AATCCACCAA AGCCAAACAC CTGCAGTTTG TCAGCGGATG CGACCCTGTC 
ACGTACTGGC TAGCAAACTA CATATGGGAC ATGCTGAACT ACCTGGTTCC AGCTACATGC 
TGTGTCCTAA TTTTGTTTGT GTTCGACCTT CCTGCGTACA CGTCACCTAC AAATTTCCCA 
GCAGTGCTCT CTCTGTTTCT GCTTTATGGC TGGTCTATAA CCCCCATCAT GTACCCAGCC 
TCCTTCTGGT TTGAGGTGCC CAGCACAGCT TATGTTTTCC TCATAGTCAT CAATCTCTTC 
ATTGGCATCA CAGCCACGGT GGCCACTTTC CTCCTCCAGC TGTTTGAGCG CGACAAGGAC 
CTGAAGTTGG TAAACAGCTA CCTGAAGTCA TGTTTTCTCA TCTTCCCCAA CTATAACCTG 
GGTCACGGAT TGATGGAGAT GGCCTATAAT GAGTATATCA ATGAATATTA CGCCAAGATA 
GGCCAGTTTG ATAAAGTGAA GTCTCCATTT GAATGGGACA TTGTGACGCG GGGACTGGTA 
GCAATGACCA TTGAGGGCTT CGTCGGCTTT CTCATTACAA TTCTGTGCCA GTACAACTTC 
CTCAGGAAAG CCCAGAGAGT GCCTGTCAAC AGTCAGCCAG TGGAGGACGA TGATGTTGAT 
GTGGCCTGTG AAAGACGCCG AGTTCTGAGG GGAGACGCTG ACAACGACAT GCTGAAGATC 
GACAACCTAA CCAAGGTGTA CAAGTCTCGT AAGATGGGCC GGATCCTGGC AGTGGACCGA 
TTGTGTCTGG GGGTCCGTCC TGGTGAATGC TTCGGTCTGC TGGGAGTGAA TGGGGCAGGA 
AAGACCACCA CCTTCAAAAT GTTGACAGGA GATGAGAGCA CAACAGGGGG AGAAGCCTTT 
ATCCAGGAAC ACAGTATCTT GCGGGAGCTG TTACGTGTGC AGCAGAGCAT CGGTTACTGC 
CCTCAGTTTG ACGCTCTGTT TGATGATCTG ACGGCTCGAG AGCATCTGGA GCTCTACACG 
CGCCTCCGCG GCATCCCATG GAAAGATGAG GAGCGGGTGG TTCAGTGGGC TTTGGAGAAG 
TTGGAGTTGT CCAAGTATGC CGACAAGCCT GCTGGCACCT ACAGCGGAGG CAACAAACGC 
AAACTCTCTA CAGCCATTGC TCTGATTGGA TACCCATCGC TCATCTTTTT GGATGAGCCC 
ACCACAGGTA TGGACCCAAA GGCTCGCCGT TTCTTATGGA ACCTAATTCT TGACATCATC 
AAGACGGGCC GCTCAGTAGT GTTGACGTCA CACAGTATGG AGGAGTGTGA AGCGCTGTGC 
ACTAGACTGG GCATCATGGT TAATGGCAGG TTCAAGTGTC TAGGAAGCAT CCAGCATCTC 
AAAAACAGAT TCGGAGATGG ATACATGATA ACAGTACGCA CTAAGACCAC CGCTAGCGTT 
AAAGAAGTGA TTCGGTTTTT TAACAGAAAT TTTCCTGAGG CAATTCTAAA GGAGCGACAC 
CACACAAAGA TTCAGTACCA GCTGAAGTCA GAGAACATCT CTCTGGCTCA GGTCTTCAGC 
AAAATGGAGC AGGTGGTGGA GGTGCTGAGC ATCGAGGATT ATTCCGTCAG CCAAACCACA 
CTGGATAACG TCTTTGTTAA CTTTGCAAAA AAGCAAAGCG ACAACTTAGA GCAGCAAGAA 
AGTTCTCCAT CTAGTGGTGG CCAGTCGCCA CTGCAGCATC TGCTCAGCCT CCTCAGGCCT 
CGTCCTGCAA ACACTGAGCT CAACGCCCTC GTGAGTGAAG AGCCTGAGGA ACTGGAGAGT 
GATGATGACG AAGGACTGAT CAGCTTTGAG GAAGAGAGGG TGCAGTTGTC CTTTAATACA 
GACACTCTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

abca2 ( XM_005165229.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165286.1 Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X1, mRNA.

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CloneID	ODa10381
Clone ID Related Accession (Same CDS sequence)	XM_005165229.3 , XM_005165229.4
Accession Version	XM_005165229.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ATP-binding cassette sub-family A member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165229.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG TGTCCTTCTA TAGCGCGGCG 
CCGCTCACCT CGGCGGGCAT CATTCCCATC ATGCAGTCGT TGTGTCCGGA TGGTCAGCGG 
GATGAGTTTG GCTTCCTGCA GTATAAAAAC TCCACTGTGA CTCAATTGCT GGAAAGGATA 
AGTGAAGTTG TGGAGCAGAA TCACTTGTTT ACATCAGACA GGCCCAGTCT GGGAGAGGAG 
CTGGAGTCTC TGCAGCAGCA CCTCGAAAGT CTGAGCTCAG CACAAGGCCC TCTAGAGAGC 
CACTACAACA CCAGCCACGG TTTCACTGTG GGAAGCGTCC TCAGGAACCA GTCGATGTTC 
CAGCAGTTTC TGATGCGAAA TCTCTCCCTG CCGAACGACA CGTCCAGCCT GCTGCTCTCC 
TCCCCCGTCA ATCTAAAAGA GGTATATAAT CTCATCTTTG GGACGTATCC CAAAGCGGGA 
TCAAAGGGAG GAGAAAGAGA TGAAGGACAG CACCAACTCA ACAGTCCTGG AGAGAAGCTT 
GTACAGCTGG AGAAGCAACT ACTTGGAGGC TGGCACAGTG TAGACGGGGG TCTGATTCAG 
AAGGCACTGA GAGATCCCAC AAAAGCTGCT CACCGTCAAG CTCTTCTCAA AGTCTTCTCT 
CAGGCGTTGG GCATCGCTGG CGGTGTAGGA GGTCAAAAAT TCGATCCTCA AGCACTGCGT 
GAGATGGAGG GAGTTCTGTT GACTGGTGCA GTGCTTGAAA GCCTTACCTG TGGGGAAGGA 
GGGAACAGTG AGATTGGCAA AATCCTATTG GTCCCTGAGA AACAACAGGC GGTTTTACAG 
GCTTACCGCA CGACTGTTTG TGGTTTAGGG GCGGGGCAGA GGGCGGAGCT TTTCAGACAG 
TTGAGTGAAG AACTGAGAGA TCAGATTGAC ACACAGAGAG TCTTTGATCA GTTGCGTCTT 
GAGCAGTCTA ATATGTCTGG TCTGCTGGAT CAGTCCCGTC TCGGGGCTCT CCTGAAGAAC 
CTGGCTGATG TGGAGCGGCT GCTGAGGGAC GTGGATCTAC TGTCAGGTCT GGCCAGACTG 
CTGCCGAAAG GGGCCTGTGC GGGTCACCAG CCTCCTCCTC AGGCCAACAC CACCTCCTGG 
AGCTCCAACT CTACCACATG GGGTCCCAAT ACAACTGACA GTTCTGTGGA GGAAGGAGAA 
GGAGCTTCTG AGAGCGAGGC AGAGGCTGAA AACCCCAAAT CACAGTTTTC TGCATTTGTT 
CAGCTGTGGG CAGGACTTCA ACCCATACTT TGTGGGAATA ATAGGATTTT CGAACCAGAA 
GCGCTGAAAC AAGGAAATAT GAGTTCTCTG GGCTTCACCA GCAAAGAACA GAGAAATTTA 
GGATTACTTG TTCATCTCAT GACTACAAAT CCCAAAATCC TCTACTCTCC CATTGACTCA 
GAGGTGGACA AGGTCATACA AAAGGCCAAT GAGACCTTTG CTTTTGTGGG GAATGTGACT 
CACTATGCCC GTGTGTGGCT CAACATCTCT GCTGAATTAA GGGCCTTTTT AGAGGAGGGT 
AAATTGCAAA ATCACCTGAT GTGGCTCCAG CAGTTTATTA CCGACCTGAA GAGGCACCAT 
GAGCTGCTGA ACGTATCAGA CAGTGAGCTG ATGAACAGTC TGCTGGAGGA GAACTTCACC 
CTGCCAAACA CAACCACCTT ACTGGAGCAG CTGCACACCA TTGATAATGC TGCTTGTGGA 
TGGACTAACT TCATGTCAAA GGTCAGTGTG GATATCTTCA GAGGCTTCCC TGATGAAGAA 
AGCATTGTCA ATTACACACT GAACCAGGCC TACCACGACA ACGTCTCAGT CTTTGCAAGT 
GTGATTTTCC AGACCAATAA AGATGGATCC CTGCCACCCC ATGTTATGTA TAAAATTCGA 
CAGAACTCGA GCTTTACCGA GAAAACCAAT GAAATCCGAC GTGCTTATTG GCGCCCAGGG 
CCAAACACAG GCGGCAAATT CTACTTCCTT TATGGCTTTG TGTGGATCCA AGATATGATT 
GAACGGGCAA TCATCAACAC CTTTGTTGGC CACGATGTTG TGGAGCCTGG AAATTACGTG 
CAGATGTTTC CTTATCCGTG CTACACCAGA GACGATTTTC TCTTTGTTAT CGAGCACATG 
ATGCCTCTGT GTATGGTCAT ATCCTGGGTC TACTCTGTGG CCATGATGAT CCAGCACATC 
GTAGCAGAGA AGGAGCAAAG ACTGAAAGAG GTAATGAAGA TGATGGGCTT GAATAACGCA 
GTGCACTGGG TGGCCTGGTT CATCACAGGC TTCGTGCAGC TTTCCATCTC AGTGACGGCC 
CTCACTGCCA TACTGAAATA CGGGAAAGTC CTCCTTCACA GCGATCCCTT CATCATATGG 
CTTTTTCTTA CAATCTACGC TGTCGCAACC ATCATGTTCT GTTTCCTGGT GTCGGTGTTG 
TACTCTAAAG CCAAACTGGC CTCGGCATGT GGAGGAATCA TCTACTTCCT GAGTTATGTG 
CCCTACATGT ATGTGGCCAT AAGAGAAGAG GTGGCTCACG ATAAGATCAC TGCCTTTGAG 
AAGTGCATAG CGTCTTTGAT GTCTACCACA GCGTTCGGTC TGGGATCCAA GTACTTTGCC 
CTGTATGAAG TGGCTGGCGT GGGAATTCAG TGGCGCACTA TTAACCAGTC ACCTGTAGAG 
GGCGATGACT TCAATCTACT GCTGTCTATG GTCATGCTCA CTATTGACGC CATTGTATAT 
GGTGTGTTGA CCTGGTATAT AGAAGCCGTC CATCCAGGGA TGTATGGACT GCCTCGGCCA 
TGGTATTTCC CCTTGCAGAA GTCCTATTGG TTAGGTAGTG GCCGCATAGA GACGTGGGAG 
TGGCCGTGGG GTGGAGGCAC CAGGCTGAGT GTTATGGAAG AGGACCAGGC TTGTGCCATG 
GAGCATAGAC GCTCAGAGGA AACCCGTGGA ATTGAAGAAG AGCCCAATCA TTTGCCGCTG 
GTGGTGTGCA TCGACAAACT GACCAAAGTC TACAAGACGG GCAGCAAACT GGCTCTAAAC 
AAGCTGAGCC TGAACCTGCA TGAGAACCAG GTTGTCTCTT TCTTGGGACA CAATGGGGCT 
GGCAAGACCA CCACTATGTC CATTCTCACG GGACTCTTTC CACCCACCTC TGGCTCTGCC 
ACCATATACG GCCATGACAT CCGCACAGAG ATGGAGCGCA TCAGGCAGAA CTTGGGCATG 
TGTCCCCAGC ACAACGTCCT GTTTGATAAG CTCAGTGTGG AGGAACATCT GTGGTTCTAC 
TCACGGCTGA AAGGCATGGC AGAGGAAGAT ATTCGCAAGG AGATGGACAA GATGATAGAG 
GATCTCGAAT TATCTAATAA ACGTCACAGT CTGGTTCAAA CCCTGTCAGG AGGGATGAAA 
AGGAAGCTGT CAGTGGCCAT TGCTTTTGTA GGTGGGTCTC GGGCAGTGAT TTTGGATGAA 
CCAACAGCAG GAGTAGACCC ATATGCCCGT CGAGCGATCT GGGACCTCAT CCTGAAATAT 
AAGCAAGGTC GCACCATCCT GCTGTCCACT CACCACATGG ATGAGGCTGA TCTGCTGGGT 
GACCGCATTG CCATCATCTC TCATGGCAAG CTCAAGTGCT GCGGTTCTCC TCTGTTCCTC 
AAGAGCACAT ATGGAGACGG ATACAAACTC ACACTTGTTA AAAAACAGAG TGACTCACAC 
ACTGCAGATC AGTCAAGCCA GTCTCCGTCC TCTTCGATAT CTCCATGTTC AGAAAACAGA 
GTAACTCAGT TCATCCGTCA GTATGTTGCT TCCTGCCTGC TGGTGTCCGA CTCCAACACT 
GAACTCTCTT ATGTCCTACC ATCAGAGGCT GTCAAGAAAG GCTGCTTTGA GAGACTCTTC 
CAGGCTCTGG AGCAGAGTTT AGACAGCTTG GCCTTGACCA GCTTTGGCGT GATGGACACC 
ACATTGGAGG AGGTGTTTCT AAAGGTGTCA GAAGAAGATC TGTCGCTGGA AAACAGCGAT 
GCAGACATGA AGGACTCTCC AGGCGGAGCA TCAGCCGGGA AGCCGTCAGG TCTGTTAGGA 
GGGCCTCAGT GTGAAGGAGC TCCAGCCGCT GCTGTGATGA GGCCAGAGGT GGAGCTCAGC 
AACCTGATGA TGTGCTCCAG GCTGAGCCCA AGCCAAGCAT CCCTGCAGTC CGGCTCTTCT 
CTGGGCTCAG TGAGAGGAGA TGAAGGGGGT CCCTATTCTG AGTTCTATGG AGACTATTGC 
CCACTGTTTG ACAACGGTCA GGATCTGGAC TCTGCCAGTC TCAGAGATGA AGAGTCATCT 
CCTCAGCCCA CTGTACTCGA GGGCCAGGGC AGCTTCAAGC TCGATGGCTG GTGGCTTAAA 
CTCCGCCAGT TTCATGGGCT GATCGTGAAA AGGTTTCACT GTGCCAAGAG GAACACCAAG 
GGCATTTTCT CTCAGATTCT GCTGCCTGCT TTCTTCGTAT GTGTGGCAAT GACTGTGGCT 
CTGTCTGTGC CTGAGATTGG TGACCTTCCT CCCCTGATCC TGTCTCCATC TCAATATCAC 
AACTACACAC AGCCTCGAGG AAACTTCATC CCCTACGCCA ATGAAGACAG ATCTCAATAC 
AGGACTAAGT TTTCTCCAGA CGCAGGCCCA CAGAGGATTG TTAACACCCT CCGTCTGCCA 
TCTGGAGTCG GTGCGACTTG TATCCTGAAG ACTCCCTTTA ACAGCACCCT GGACCAACTG 
GCCCAAACTC TCAATCCTTA TGCCAACAAC TCTAAAACCC TGGCAGCCCG CTACTTTGAC 
TCTATGTGCC TGGACTCCTT CACGCAGGGT GTGCCTTTGT CGAATTTCGT TCCCCCTCCG 
CCCTCTCCTG CCCCCTCAGA CGACCCGGAG GCTCATTTTG AAGACGGCCT GTGGAACTAC 
ACTGCAGCTC CTACTACTGT CAGAGAGATG GCCACGTCTC CCCCGACTCT GCCCTTGAGT 
ATCCATGAGC CGGTGCGCTG TATCTGCTCT ATGCAGGGCA CAGGCTTCTC CTGCCCCAGT 
GGAGTTGGGG GTCGACCTCC TCTGATGAAA GTGGTGACCG GAGACATCCT GGTGGACATT 
ACAGGTCGCA ACGTGTCTGA ATATCTCCTG TACACGTCTG ATCGGCTCAG GTTACACAGA 
TATGGAGGCT TTACTGTTGG AAACATTCAG AAATCTGTCC CAGCATCCTT CGGGAGAAAA 
ACACCACCTA TGGTGCGCAA AATAGCAGTG AGGCGATCCT CACAGGTGCT GTATAATAAC 
AAAGGCTATC ACAGTATGCC AACATATCTA AATGTCCTCA ACAATGCCAT TCTCAGAGCC 
AATCTGCCCA GTAGTAAAGG AAACCCTTCT GCATACGGCA TTACAGTGAC CAATCATCCC 
ATGAACAGAA CCAGTGCCAG TCTTTCTCTG GATTATCTGC TCCAGGGCAC TGATGTGGTT 
ATTGCCATCT TCATCATTGT AGCCATGTCT TTCGTGCCTG CGAGCTTTGT GGTCTTTCTG 
GTAGCGGAAA AATCCACCAA AGCCAAACAC CTGCAGTTTG TCAGCGGATG CGACCCTGTC 
ACGTACTGGC TAGCAAACTA CATATGGGAC ATGCTGAACT ACCTGGTTCC AGCTACATGC 
TGTGTCCTAA TTTTGTTTGT GTTCGACCTT CCTGCGTACA CGTCACCTAC AAATTTCCCA 
GCAGTGCTCT CTCTGTTTCT GCTTTATGGC TGGTCTATAA CCCCCATCAT GTACCCAGCC 
TCCTTCTGGT TTGAGGTGCC CAGCACAGCT TATGTTTTCC TCATAGTCAT CAATCTCTTC 
ATTGGCATCA CAGCCACGGT GGCCACTTTC CTCCTCCAGC TGTTTGAGCG CGACAAGGAC 
CTGAAGTTGG TAAACAGCTA CCTGAAGTCA TGTTTTCTCA TCTTCCCCAA CTATAACCTG 
GGTCACGGAT TGATGGAGAT GGCCTATAAT GAGTATATCA ATGAATATTA CGCCAAGATA 
GGCCAGTTTG ATAAAGTGAA GTCTCCATTT GAATGGGACA TTGTGACGCG GGGACTGGTA 
GCAATGACCA TTGAGGGCTT CGTCGGCTTT CTCATTACAA TTCTGTGCCA GTACAACTTC 
CTCAGGAAAG CCCAGAGAGT GCCTGTCAAC AGTCAGCCAG TGGAGGACGA TGATGTTGAT 
GTGGCCTGTG AAAGACGCCG AGTTCTGAGG GGAGACGCTG ACAACGACAT GCTGAAGATC 
GACAACCTAA CCAAGGTGTA CAAGTCTCGT AAGATGGGCC GGATCCTGGC AGTGGACCGA 
TTGTGTCTGG GGGTCCGTCC TGGTGAATGC TTCGGTCTGC TGGGAGTGAA TGGGGCAGGA 
AAGACCACCA CCTTCAAAAT GTTGACAGGA GATGAGAGCA CAACAGGGGG AGAAGCCTTT 
ATCCAGGAAC ACAGTATCTT GCGGGAGCTG TTACGTGTGC AGCAGAGCAT CGGTTACTGC 
CCTCAGTTTG ACGCTCTGTT TGATGATCTG ACGGCTCGAG AGCATCTGGA GCTCTACACG 
CGCCTCCGCG GCATCCCATG GAAAGATGAG GAGCGGGTGG TTCAGTGGGC TTTGGAGAAG 
TTGGAGTTGT CCAAGTATGC CGACAAGCCT GCTGGCACCT ACAGCGGAGG CAACAAACGC 
AAACTCTCTA CAGCCATTGC TCTGATTGGA TACCCATCGC TCATCTTTTT GGATGAGCCC 
ACCACAGGTA TGGACCCAAA GGCTCGCCGT TTCTTATGGA ACCTAATTCT TGACATCATC 
AAGACGGGCC GCTCAGTAGT GTTGACGTCA CACAGTATGG AGGAGTGTGA AGCGCTGTGC 
ACTAGACTGG GCATCATGGT TAATGGCAGG TTCAAGTGTC TAGGAAGCAT CCAGCATCTC 
AAAAACAGAT TCGGAGATGG ATACATGATA ACAGTACGCA CTAAGACCAC CGCTAGCGTT 
AAAGAAGTGA TTCGGTTTTT TAACAGAAAT TTTCCTGAGG CAATTCTAAA GGAGCGACAC 
CACACAAAGA TTCAGTACCA GCTGAAGTCA GAGAACATCT CTCTGGCTCA GGTCTTCAGC 
AAAATGGAGC AGGTGGTGGA GGTGCTGAGC ATCGAGGATT ATTCCGTCAG CCAAACCACA 
CTGGATAACG TCTTTGTTAA CTTTGCAAAA AAGCAAAGCG ACAACTTAGA GCAGCAAGAA 
AGTTCTCCAT CTAGTGGTGG CCAGTCGCCA CTGCAGCATC TGCTCAGCCT CCTCAGGCCT 
CGTCCTGCAA ACACTGAGCT CAACGCCCTC GTGAGTGAAG AGCCTGAGGA ACTGGAGAGT 
GATGATGACG AAGGACTGAT CAGCTTTGAG GAAGAGAGGG TGCAGTTGTC CTTTAATACA 
GACACTCTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165286.1
CDS	554..8068
Translation	MGFLHQLHLLLWKNVTLKRRGPWVLAFEIFIPLVLFFILLGLRQKKPAIPVKEVSFYSAAPLTSAGIIPIMQSLCPDGQRDEFGFLQYKNSTVTQLLERISEVVEQNHLFTSDRPSLGEELESLQQHLESLSSAQGPLESHYNTSHGFTVGSVLRNQSMFQQFLMRNLSLPNDTSSLLLSSPVNLKEVYNLIFGTYPKAGSKGGERDEGQHQLNSPGEKLVQLEKQLLGGWHSVDGGLIQKALRDPTKAAHRQALLKVFSQALGIAGGVGGQKFDPQALREMEGVLLTGAVLESLTCGEGGNSEIGKILLVPEKQQAVLQAYRTTVCGLGAGQRAELFRQLSEELRDQIDTQRVFDQLRLEQSNMSGLLDQSRLGALLKNLADVERLLRDVDLLSGLARLLPKGACAGHQPPPQANTTSWSSNSTTWGPNTTDSSVEEGEGASESEAEAENPKSQFSAFVQLWAGLQPILCGNNRIFEPEALKQGNMSSLGFTSKEQRNLGLLVHLMTTNPKILYSPIDSEVDKVIQKANETFAFVGNVTHYARVWLNISAELRAFLEEGKLQNHLMWLQQFITDLKRHHELLNVSDSELMNSLLEENFTLPNTTTLLEQLHTIDNAACGWTNFMSKVSVDIFRGFPDEESIVNYTLNQAYHDNVSVFASVIFQTNKDGSLPPHVMYKIRQNSSFTEKTNEIRRAYWRPGPNTGGKFYFLYGFVWIQDMIERAIINTFVGHDVVEPGNYVQMFPYPCYTRDDFLFVIEHMMPLCMVISWVYSVAMMIQHIVAEKEQRLKEVMKMMGLNNAVHWVAWFITGFVQLSISVTALTAILKYGKVLLHSDPFIIWLFLTIYAVATIMFCFLVSVLYSKAKLASACGGIIYFLSYVPYMYVAIREEVAHDKITAFEKCIASLMSTTAFGLGSKYFALYEVAGVGIQWRTINQSPVEGDDFNLLLSMVMLTIDAIVYGVLTWYIEAVHPGMYGLPRPWYFPLQKSYWLGSGRIETWEWPWGGGTRLSVMEEDQACAMEHRRSEETRGIEEEPNHLPLVVCIDKLTKVYKTGSKLALNKLSLNLHENQVVSFLGHNGAGKTTTMSILTGLFPPTSGSATIYGHDIRTEMERIRQNLGMCPQHNVLFDKLSVEEHLWFYSRLKGMAEEDIRKEMDKMIEDLELSNKRHSLVQTLSGGMKRKLSVAIAFVGGSRAVILDEPTAGVDPYARRAIWDLILKYKQGRTILLSTHHMDEADLLGDRIAIISHGKLKCCGSPLFLKSTYGDGYKLTLVKKQSDSHTADQSSQSPSSSISPCSENRVTQFIRQYVASCLLVSDSNTELSYVLPSEAVKKGCFERLFQALEQSLDSLALTSFGVMDTTLEEVFLKVSEEDLSLENSDADMKDSPGGASAGKPSGLLGGPQCEGAPAAAVMRPEVELSNLMMCSRLSPSQASLQSGSSLGSVRGDEGGPYSEFYGDYCPLFDNGQDLDSASLRDEESSPQPTVLEGQGSFKLDGWWLKLRQFHGLIVKRFHCAKRNTKGIFSQILLPAFFVCVAMTVALSVPEIGDLPPLILSPSQYHNYTQPRGNFIPYANEDRSQYRTKFSPDAGPQRIVNTLRLPSGVGATCILKTPFNSTLDQLAQTLNPYANNSKTLAARYFDSMCLDSFTQGVPLSNFVPPPPSPAPSDDPEAHFEDGLWNYTAAPTTVREMATSPPTLPLSIHEPVRCICSMQGTGFSCPSGVGGRPPLMKVVTGDILVDITGRNVSEYLLYTSDRLRLHRYGGFTVGNIQKSVPASFGRKTPPMVRKIAVRRSSQVLYNNKGYHSMPTYLNVLNNAILRANLPSSKGNPSAYGITVTNHPMNRTSASLSLDYLLQGTDVVIAIFIIVAMSFVPASFVVFLVAEKSTKAKHLQFVSGCDPVTYWLANYIWDMLNYLVPATCCVLILFVFDLPAYTSPTNFPAVLSLFLLYGWSITPIMYPASFWFEVPSTAYVFLIVINLFIGITATVATFLLQLFERDKDLKLVNSYLKSCFLIFPNYNLGHGLMEMAYNEYINEYYAKIGQFDKVKSPFEWDIVTRGLVAMTIEGFVGFLITILCQYNFLRKAQRVPVNSQPVEDDDVDVACERRRVLRGDADNDMLKIDNLTKVYKSRKMGRILAVDRLCLGVRPGECFGLLGVNGAGKTTTFKMLTGDESTTGGEAFIQEHSILRELLRVQQSIGYCPQFDALFDDLTAREHLELYTRLRGIPWKDEERVVQWALEKLELSKYADKPAGTYSGGNKRKLSTAIALIGYPSLIFLDEPTTGMDPKARRFLWNLILDIIKTGRSVVLTSHSMEECEALCTRLGIMVNGRFKCLGSIQHLKNRFGDGYMITVRTKTTASVKEVIRFFNRNFPEAILKERHHTKIQYQLKSENISLAQVFSKMEQVVEVLSIEDYSVSQTTLDNVFVNFAKKQSDNLEQQESSPSSGGQSPLQHLLSLLRPRPANTELNALVSEEPEELESDDDEGLISFEEERVQLSFNTDTLC

Target ORF information:

RefSeq Version	XM_005165229.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165229.4

ORF Insert Sequence:

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG TGTCCTTCTA TAGCGCGGCG 
CCGCTCACCT CGGCGGGCAT CATTCCCATC ATGCAGTCGT TGTGTCCGGA TGGTCAGCGG 
GATGAGTTTG GCTTCCTGCA GTATAAAAAC TCCACTGTGA CTCAATTGCT GGAAAGGATA 
AGTGAAGTTG TGGAGCAGAA TCACTTGTTT ACATCAGACA GGCCCAGTCT GGGAGAGGAG 
CTGGAGTCTC TGCAGCAGCA CCTCGAAAGT CTGAGCTCAG CACAAGGCCC TCTAGAGAGC 
CACTACAACA CCAGCCACGG TTTCACTGTG GGAAGCGTCC TCAGGAACCA GTCGATGTTC 
CAGCAGTTTC TGATGCGAAA TCTCTCCCTG CCGAACGACA CGTCCAGCCT GCTGCTCTCC 
TCCCCCGTCA ATCTAAAAGA GGTATATAAT CTCATCTTTG GGACGTATCC CAAAGCGGGA 
TCAAAGGGAG GAGAAAGAGA TGAAGGACAG CACCAACTCA ACAGTCCTGG AGAGAAGCTT 
GTACAGCTGG AGAAGCAACT ACTTGGAGGC TGGCACAGTG TAGACGGGGG TCTGATTCAG 
AAGGCACTGA GAGATCCCAC AAAAGCTGCT CACCGTCAAG CTCTTCTCAA AGTCTTCTCT 
CAGGCGTTGG GCATCGCTGG CGGTGTAGGA GGTCAAAAAT TCGATCCTCA AGCACTGCGT 
GAGATGGAGG GAGTTCTGTT GACTGGTGCA GTGCTTGAAA GCCTTACCTG TGGGGAAGGA 
GGGAACAGTG AGATTGGCAA AATCCTATTG GTCCCTGAGA AACAACAGGC GGTTTTACAG 
GCTTACCGCA CGACTGTTTG TGGTTTAGGG GCGGGGCAGA GGGCGGAGCT TTTCAGACAG 
TTGAGTGAAG AACTGAGAGA TCAGATTGAC ACACAGAGAG TCTTTGATCA GTTGCGTCTT 
GAGCAGTCTA ATATGTCTGG TCTGCTGGAT CAGTCCCGTC TCGGGGCTCT CCTGAAGAAC 
CTGGCTGATG TGGAGCGGCT GCTGAGGGAC GTGGATCTAC TGTCAGGTCT GGCCAGACTG 
CTGCCGAAAG GGGCCTGTGC GGGTCACCAG CCTCCTCCTC AGGCCAACAC CACCTCCTGG 
AGCTCCAACT CTACCACATG GGGTCCCAAT ACAACTGACA GTTCTGTGGA GGAAGGAGAA 
GGAGCTTCTG AGAGCGAGGC AGAGGCTGAA AACCCCAAAT CACAGTTTTC TGCATTTGTT 
CAGCTGTGGG CAGGACTTCA ACCCATACTT TGTGGGAATA ATAGGATTTT CGAACCAGAA 
GCGCTGAAAC AAGGAAATAT GAGTTCTCTG GGCTTCACCA GCAAAGAACA GAGAAATTTA 
GGATTACTTG TTCATCTCAT GACTACAAAT CCCAAAATCC TCTACTCTCC CATTGACTCA 
GAGGTGGACA AGGTCATACA AAAGGCCAAT GAGACCTTTG CTTTTGTGGG GAATGTGACT 
CACTATGCCC GTGTGTGGCT CAACATCTCT GCTGAATTAA GGGCCTTTTT AGAGGAGGGT 
AAATTGCAAA ATCACCTGAT GTGGCTCCAG CAGTTTATTA CCGACCTGAA GAGGCACCAT 
GAGCTGCTGA ACGTATCAGA CAGTGAGCTG ATGAACAGTC TGCTGGAGGA GAACTTCACC 
CTGCCAAACA CAACCACCTT ACTGGAGCAG CTGCACACCA TTGATAATGC TGCTTGTGGA 
TGGACTAACT TCATGTCAAA GGTCAGTGTG GATATCTTCA GAGGCTTCCC TGATGAAGAA 
AGCATTGTCA ATTACACACT GAACCAGGCC TACCACGACA ACGTCTCAGT CTTTGCAAGT 
GTGATTTTCC AGACCAATAA AGATGGATCC CTGCCACCCC ATGTTATGTA TAAAATTCGA 
CAGAACTCGA GCTTTACCGA GAAAACCAAT GAAATCCGAC GTGCTTATTG GCGCCCAGGG 
CCAAACACAG GCGGCAAATT CTACTTCCTT TATGGCTTTG TGTGGATCCA AGATATGATT 
GAACGGGCAA TCATCAACAC CTTTGTTGGC CACGATGTTG TGGAGCCTGG AAATTACGTG 
CAGATGTTTC CTTATCCGTG CTACACCAGA GACGATTTTC TCTTTGTTAT CGAGCACATG 
ATGCCTCTGT GTATGGTCAT ATCCTGGGTC TACTCTGTGG CCATGATGAT CCAGCACATC 
GTAGCAGAGA AGGAGCAAAG ACTGAAAGAG GTAATGAAGA TGATGGGCTT GAATAACGCA 
GTGCACTGGG TGGCCTGGTT CATCACAGGC TTCGTGCAGC TTTCCATCTC AGTGACGGCC 
CTCACTGCCA TACTGAAATA CGGGAAAGTC CTCCTTCACA GCGATCCCTT CATCATATGG 
CTTTTTCTTA CAATCTACGC TGTCGCAACC ATCATGTTCT GTTTCCTGGT GTCGGTGTTG 
TACTCTAAAG CCAAACTGGC CTCGGCATGT GGAGGAATCA TCTACTTCCT GAGTTATGTG 
CCCTACATGT ATGTGGCCAT AAGAGAAGAG GTGGCTCACG ATAAGATCAC TGCCTTTGAG 
AAGTGCATAG CGTCTTTGAT GTCTACCACA GCGTTCGGTC TGGGATCCAA GTACTTTGCC 
CTGTATGAAG TGGCTGGCGT GGGAATTCAG TGGCGCACTA TTAACCAGTC ACCTGTAGAG 
GGCGATGACT TCAATCTACT GCTGTCTATG GTCATGCTCA CTATTGACGC CATTGTATAT 
GGTGTGTTGA CCTGGTATAT AGAAGCCGTC CATCCAGGGA TGTATGGACT GCCTCGGCCA 
TGGTATTTCC CCTTGCAGAA GTCCTATTGG TTAGGTAGTG GCCGCATAGA GACGTGGGAG 
TGGCCGTGGG GTGGAGGCAC CAGGCTGAGT GTTATGGAAG AGGACCAGGC TTGTGCCATG 
GAGCATAGAC GCTCAGAGGA AACCCGTGGA ATTGAAGAAG AGCCCAATCA TTTGCCGCTG 
GTGGTGTGCA TCGACAAACT GACCAAAGTC TACAAGACGG GCAGCAAACT GGCTCTAAAC 
AAGCTGAGCC TGAACCTGCA TGAGAACCAG GTTGTCTCTT TCTTGGGACA CAATGGGGCT 
GGCAAGACCA CCACTATGTC CATTCTCACG GGACTCTTTC CACCCACCTC TGGCTCTGCC 
ACCATATACG GCCATGACAT CCGCACAGAG ATGGAGCGCA TCAGGCAGAA CTTGGGCATG 
TGTCCCCAGC ACAACGTCCT GTTTGATAAG CTCAGTGTGG AGGAACATCT GTGGTTCTAC 
TCACGGCTGA AAGGCATGGC AGAGGAAGAT ATTCGCAAGG AGATGGACAA GATGATAGAG 
GATCTCGAAT TATCTAATAA ACGTCACAGT CTGGTTCAAA CCCTGTCAGG AGGGATGAAA 
AGGAAGCTGT CAGTGGCCAT TGCTTTTGTA GGTGGGTCTC GGGCAGTGAT TTTGGATGAA 
CCAACAGCAG GAGTAGACCC ATATGCCCGT CGAGCGATCT GGGACCTCAT CCTGAAATAT 
AAGCAAGGTC GCACCATCCT GCTGTCCACT CACCACATGG ATGAGGCTGA TCTGCTGGGT 
GACCGCATTG CCATCATCTC TCATGGCAAG CTCAAGTGCT GCGGTTCTCC TCTGTTCCTC 
AAGAGCACAT ATGGAGACGG ATACAAACTC ACACTTGTTA AAAAACAGAG TGACTCACAC 
ACTGCAGATC AGTCAAGCCA GTCTCCGTCC TCTTCGATAT CTCCATGTTC AGAAAACAGA 
GTAACTCAGT TCATCCGTCA GTATGTTGCT TCCTGCCTGC TGGTGTCCGA CTCCAACACT 
GAACTCTCTT ATGTCCTACC ATCAGAGGCT GTCAAGAAAG GCTGCTTTGA GAGACTCTTC 
CAGGCTCTGG AGCAGAGTTT AGACAGCTTG GCCTTGACCA GCTTTGGCGT GATGGACACC 
ACATTGGAGG AGGTGTTTCT AAAGGTGTCA GAAGAAGATC TGTCGCTGGA AAACAGCGAT 
GCAGACATGA AGGACTCTCC AGGCGGAGCA TCAGCCGGGA AGCCGTCAGG TCTGTTAGGA 
GGGCCTCAGT GTGAAGGAGC TCCAGCCGCT GCTGTGATGA GGCCAGAGGT GGAGCTCAGC 
AACCTGATGA TGTGCTCCAG GCTGAGCCCA AGCCAAGCAT CCCTGCAGTC CGGCTCTTCT 
CTGGGCTCAG TGAGAGGAGA TGAAGGGGGT CCCTATTCTG AGTTCTATGG AGACTATTGC 
CCACTGTTTG ACAACGGTCA GGATCTGGAC TCTGCCAGTC TCAGAGATGA AGAGTCATCT 
CCTCAGCCCA CTGTACTCGA GGGCCAGGGC AGCTTCAAGC TCGATGGCTG GTGGCTTAAA 
CTCCGCCAGT TTCATGGGCT GATCGTGAAA AGGTTTCACT GTGCCAAGAG GAACACCAAG 
GGCATTTTCT CTCAGATTCT GCTGCCTGCT TTCTTCGTAT GTGTGGCAAT GACTGTGGCT 
CTGTCTGTGC CTGAGATTGG TGACCTTCCT CCCCTGATCC TGTCTCCATC TCAATATCAC 
AACTACACAC AGCCTCGAGG AAACTTCATC CCCTACGCCA ATGAAGACAG ATCTCAATAC 
AGGACTAAGT TTTCTCCAGA CGCAGGCCCA CAGAGGATTG TTAACACCCT CCGTCTGCCA 
TCTGGAGTCG GTGCGACTTG TATCCTGAAG ACTCCCTTTA ACAGCACCCT GGACCAACTG 
GCCCAAACTC TCAATCCTTA TGCCAACAAC TCTAAAACCC TGGCAGCCCG CTACTTTGAC 
TCTATGTGCC TGGACTCCTT CACGCAGGGT GTGCCTTTGT CGAATTTCGT TCCCCCTCCG 
CCCTCTCCTG CCCCCTCAGA CGACCCGGAG GCTCATTTTG AAGACGGCCT GTGGAACTAC 
ACTGCAGCTC CTACTACTGT CAGAGAGATG GCCACGTCTC CCCCGACTCT GCCCTTGAGT 
ATCCATGAGC CGGTGCGCTG TATCTGCTCT ATGCAGGGCA CAGGCTTCTC CTGCCCCAGT 
GGAGTTGGGG GTCGACCTCC TCTGATGAAA GTGGTGACCG GAGACATCCT GGTGGACATT 
ACAGGTCGCA ACGTGTCTGA ATATCTCCTG TACACGTCTG ATCGGCTCAG GTTACACAGA 
TATGGAGGCT TTACTGTTGG AAACATTCAG AAATCTGTCC CAGCATCCTT CGGGAGAAAA 
ACACCACCTA TGGTGCGCAA AATAGCAGTG AGGCGATCCT CACAGGTGCT GTATAATAAC 
AAAGGCTATC ACAGTATGCC AACATATCTA AATGTCCTCA ACAATGCCAT TCTCAGAGCC 
AATCTGCCCA GTAGTAAAGG AAACCCTTCT GCATACGGCA TTACAGTGAC CAATCATCCC 
ATGAACAGAA CCAGTGCCAG TCTTTCTCTG GATTATCTGC TCCAGGGCAC TGATGTGGTT 
ATTGCCATCT TCATCATTGT AGCCATGTCT TTCGTGCCTG CGAGCTTTGT GGTCTTTCTG 
GTAGCGGAAA AATCCACCAA AGCCAAACAC CTGCAGTTTG TCAGCGGATG CGACCCTGTC 
ACGTACTGGC TAGCAAACTA CATATGGGAC ATGCTGAACT ACCTGGTTCC AGCTACATGC 
TGTGTCCTAA TTTTGTTTGT GTTCGACCTT CCTGCGTACA CGTCACCTAC AAATTTCCCA 
GCAGTGCTCT CTCTGTTTCT GCTTTATGGC TGGTCTATAA CCCCCATCAT GTACCCAGCC 
TCCTTCTGGT TTGAGGTGCC CAGCACAGCT TATGTTTTCC TCATAGTCAT CAATCTCTTC 
ATTGGCATCA CAGCCACGGT GGCCACTTTC CTCCTCCAGC TGTTTGAGCG CGACAAGGAC 
CTGAAGTTGG TAAACAGCTA CCTGAAGTCA TGTTTTCTCA TCTTCCCCAA CTATAACCTG 
GGTCACGGAT TGATGGAGAT GGCCTATAAT GAGTATATCA ATGAATATTA CGCCAAGATA 
GGCCAGTTTG ATAAAGTGAA GTCTCCATTT GAATGGGACA TTGTGACGCG GGGACTGGTA 
GCAATGACCA TTGAGGGCTT CGTCGGCTTT CTCATTACAA TTCTGTGCCA GTACAACTTC 
CTCAGGAAAG CCCAGAGAGT GCCTGTCAAC AGTCAGCCAG TGGAGGACGA TGATGTTGAT 
GTGGCCTGTG AAAGACGCCG AGTTCTGAGG GGAGACGCTG ACAACGACAT GCTGAAGATC 
GACAACCTAA CCAAGGTGTA CAAGTCTCGT AAGATGGGCC GGATCCTGGC AGTGGACCGA 
TTGTGTCTGG GGGTCCGTCC TGGTGAATGC TTCGGTCTGC TGGGAGTGAA TGGGGCAGGA 
AAGACCACCA CCTTCAAAAT GTTGACAGGA GATGAGAGCA CAACAGGGGG AGAAGCCTTT 
ATCCAGGAAC ACAGTATCTT GCGGGAGCTG TTACGTGTGC AGCAGAGCAT CGGTTACTGC 
CCTCAGTTTG ACGCTCTGTT TGATGATCTG ACGGCTCGAG AGCATCTGGA GCTCTACACG 
CGCCTCCGCG GCATCCCATG GAAAGATGAG GAGCGGGTGG TTCAGTGGGC TTTGGAGAAG 
TTGGAGTTGT CCAAGTATGC CGACAAGCCT GCTGGCACCT ACAGCGGAGG CAACAAACGC 
AAACTCTCTA CAGCCATTGC TCTGATTGGA TACCCATCGC TCATCTTTTT GGATGAGCCC 
ACCACAGGTA TGGACCCAAA GGCTCGCCGT TTCTTATGGA ACCTAATTCT TGACATCATC 
AAGACGGGCC GCTCAGTAGT GTTGACGTCA CACAGTATGG AGGAGTGTGA AGCGCTGTGC 
ACTAGACTGG GCATCATGGT TAATGGCAGG TTCAAGTGTC TAGGAAGCAT CCAGCATCTC 
AAAAACAGAT TCGGAGATGG ATACATGATA ACAGTACGCA CTAAGACCAC CGCTAGCGTT 
AAAGAAGTGA TTCGGTTTTT TAACAGAAAT TTTCCTGAGG CAATTCTAAA GGAGCGACAC 
CACACAAAGA TTCAGTACCA GCTGAAGTCA GAGAACATCT CTCTGGCTCA GGTCTTCAGC 
AAAATGGAGC AGGTGGTGGA GGTGCTGAGC ATCGAGGATT ATTCCGTCAG CCAAACCACA 
CTGGATAACG TCTTTGTTAA CTTTGCAAAA AAGCAAAGCG ACAACTTAGA GCAGCAAGAA 
AGTTCTCCAT CTAGTGGTGG CCAGTCGCCA CTGCAGCATC TGCTCAGCCT CCTCAGGCCT 
CGTCCTGCAA ACACTGAGCT CAACGCCCTC GTGAGTGAAG AGCCTGAGGA ACTGGAGAGT 
GATGATGACG AAGGACTGAT CAGCTTTGAG GAAGAGAGGG TGCAGTTGTC CTTTAATACA 
GACACTCTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

abca2 ( XM_005165230.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165287.1 Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X2, mRNA.

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CloneID	ODa10382
Clone ID Related Accession (Same CDS sequence)	XM_005165230.4 , XM_005165230.3
Accession Version	XM_005165230.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ATP-binding cassette sub-family A member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165230.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG CCTTCTATAG CGCGGCGCCG 
CTCACCTCGG CGGGCATCAT TCCCATCATG CAGTCGTTGT GTCCGGATGG TCAGCGGGAT 
GAGTTTGGCT TCCTGCAGTA TAAAAACTCC ACTGTGACTC AATTGCTGGA AAGGATAAGT 
GAAGTTGTGG AGCAGAATCA CTTGTTTACA TCAGACAGGC CCAGTCTGGG AGAGGAGCTG 
GAGTCTCTGC AGCAGCACCT CGAAAGTCTG AGCTCAGCAC AAGGCCCTCT AGAGAGCCAC 
TACAACACCA GCCACGGTTT CACTGTGGGA AGCGTCCTCA GGAACCAGTC GATGTTCCAG 
CAGTTTCTGA TGCGAAATCT CTCCCTGCCG AACGACACGT CCAGCCTGCT GCTCTCCTCC 
CCCGTCAATC TAAAAGAGGT ATATAATCTC ATCTTTGGGA CGTATCCCAA AGCGGGATCA 
AAGGGAGGAG AAAGAGATGA AGGACAGCAC CAACTCAACA GTCCTGGAGA GAAGCTTGTA 
CAGCTGGAGA AGCAACTACT TGGAGGCTGG CACAGTGTAG ACGGGGGTCT GATTCAGAAG 
GCACTGAGAG ATCCCACAAA AGCTGCTCAC CGTCAAGCTC TTCTCAAAGT CTTCTCTCAG 
GCGTTGGGCA TCGCTGGCGG TGTAGGAGGT CAAAAATTCG ATCCTCAAGC ACTGCGTGAG 
ATGGAGGGAG TTCTGTTGAC TGGTGCAGTG CTTGAAAGCC TTACCTGTGG GGAAGGAGGG 
AACAGTGAGA TTGGCAAAAT CCTATTGGTC CCTGAGAAAC AACAGGCGGT TTTACAGGCT 
TACCGCACGA CTGTTTGTGG TTTAGGGGCG GGGCAGAGGG CGGAGCTTTT CAGACAGTTG 
AGTGAAGAAC TGAGAGATCA GATTGACACA CAGAGAGTCT TTGATCAGTT GCGTCTTGAG 
CAGTCTAATA TGTCTGGTCT GCTGGATCAG TCCCGTCTCG GGGCTCTCCT GAAGAACCTG 
GCTGATGTGG AGCGGCTGCT GAGGGACGTG GATCTACTGT CAGGTCTGGC CAGACTGCTG 
CCGAAAGGGG CCTGTGCGGG TCACCAGCCT CCTCCTCAGG CCAACACCAC CTCCTGGAGC 
TCCAACTCTA CCACATGGGG TCCCAATACA ACTGACAGTT CTGTGGAGGA AGGAGAAGGA 
GCTTCTGAGA GCGAGGCAGA GGCTGAAAAC CCCAAATCAC AGTTTTCTGC ATTTGTTCAG 
CTGTGGGCAG GACTTCAACC CATACTTTGT GGGAATAATA GGATTTTCGA ACCAGAAGCG 
CTGAAACAAG GAAATATGAG TTCTCTGGGC TTCACCAGCA AAGAACAGAG AAATTTAGGA 
TTACTTGTTC ATCTCATGAC TACAAATCCC AAAATCCTCT ACTCTCCCAT TGACTCAGAG 
GTGGACAAGG TCATACAAAA GGCCAATGAG ACCTTTGCTT TTGTGGGGAA TGTGACTCAC 
TATGCCCGTG TGTGGCTCAA CATCTCTGCT GAATTAAGGG CCTTTTTAGA GGAGGGTAAA 
TTGCAAAATC ACCTGATGTG GCTCCAGCAG TTTATTACCG ACCTGAAGAG GCACCATGAG 
CTGCTGAACG TATCAGACAG TGAGCTGATG AACAGTCTGC TGGAGGAGAA CTTCACCCTG 
CCAAACACAA CCACCTTACT GGAGCAGCTG CACACCATTG ATAATGCTGC TTGTGGATGG 
ACTAACTTCA TGTCAAAGGT CAGTGTGGAT ATCTTCAGAG GCTTCCCTGA TGAAGAAAGC 
ATTGTCAATT ACACACTGAA CCAGGCCTAC CACGACAACG TCTCAGTCTT TGCAAGTGTG 
ATTTTCCAGA CCAATAAAGA TGGATCCCTG CCACCCCATG TTATGTATAA AATTCGACAG 
AACTCGAGCT TTACCGAGAA AACCAATGAA ATCCGACGTG CTTATTGGCG CCCAGGGCCA 
AACACAGGCG GCAAATTCTA CTTCCTTTAT GGCTTTGTGT GGATCCAAGA TATGATTGAA 
CGGGCAATCA TCAACACCTT TGTTGGCCAC GATGTTGTGG AGCCTGGAAA TTACGTGCAG 
ATGTTTCCTT ATCCGTGCTA CACCAGAGAC GATTTTCTCT TTGTTATCGA GCACATGATG 
CCTCTGTGTA TGGTCATATC CTGGGTCTAC TCTGTGGCCA TGATGATCCA GCACATCGTA 
GCAGAGAAGG AGCAAAGACT GAAAGAGGTA ATGAAGATGA TGGGCTTGAA TAACGCAGTG 
CACTGGGTGG CCTGGTTCAT CACAGGCTTC GTGCAGCTTT CCATCTCAGT GACGGCCCTC 
ACTGCCATAC TGAAATACGG GAAAGTCCTC CTTCACAGCG ATCCCTTCAT CATATGGCTT 
TTTCTTACAA TCTACGCTGT CGCAACCATC ATGTTCTGTT TCCTGGTGTC GGTGTTGTAC 
TCTAAAGCCA AACTGGCCTC GGCATGTGGA GGAATCATCT ACTTCCTGAG TTATGTGCCC 
TACATGTATG TGGCCATAAG AGAAGAGGTG GCTCACGATA AGATCACTGC CTTTGAGAAG 
TGCATAGCGT CTTTGATGTC TACCACAGCG TTCGGTCTGG GATCCAAGTA CTTTGCCCTG 
TATGAAGTGG CTGGCGTGGG AATTCAGTGG CGCACTATTA ACCAGTCACC TGTAGAGGGC 
GATGACTTCA ATCTACTGCT GTCTATGGTC ATGCTCACTA TTGACGCCAT TGTATATGGT 
GTGTTGACCT GGTATATAGA AGCCGTCCAT CCAGGGATGT ATGGACTGCC TCGGCCATGG 
TATTTCCCCT TGCAGAAGTC CTATTGGTTA GGTAGTGGCC GCATAGAGAC GTGGGAGTGG 
CCGTGGGGTG GAGGCACCAG GCTGAGTGTT ATGGAAGAGG ACCAGGCTTG TGCCATGGAG 
CATAGACGCT CAGAGGAAAC CCGTGGAATT GAAGAAGAGC CCAATCATTT GCCGCTGGTG 
GTGTGCATCG ACAAACTGAC CAAAGTCTAC AAGACGGGCA GCAAACTGGC TCTAAACAAG 
CTGAGCCTGA ACCTGCATGA GAACCAGGTT GTCTCTTTCT TGGGACACAA TGGGGCTGGC 
AAGACCACCA CTATGTCCAT TCTCACGGGA CTCTTTCCAC CCACCTCTGG CTCTGCCACC 
ATATACGGCC ATGACATCCG CACAGAGATG GAGCGCATCA GGCAGAACTT GGGCATGTGT 
CCCCAGCACA ACGTCCTGTT TGATAAGCTC AGTGTGGAGG AACATCTGTG GTTCTACTCA 
CGGCTGAAAG GCATGGCAGA GGAAGATATT CGCAAGGAGA TGGACAAGAT GATAGAGGAT 
CTCGAATTAT CTAATAAACG TCACAGTCTG GTTCAAACCC TGTCAGGAGG GATGAAAAGG 
AAGCTGTCAG TGGCCATTGC TTTTGTAGGT GGGTCTCGGG CAGTGATTTT GGATGAACCA 
ACAGCAGGAG TAGACCCATA TGCCCGTCGA GCGATCTGGG ACCTCATCCT GAAATATAAG 
CAAGGTCGCA CCATCCTGCT GTCCACTCAC CACATGGATG AGGCTGATCT GCTGGGTGAC 
CGCATTGCCA TCATCTCTCA TGGCAAGCTC AAGTGCTGCG GTTCTCCTCT GTTCCTCAAG 
AGCACATATG GAGACGGATA CAAACTCACA CTTGTTAAAA AACAGAGTGA CTCACACACT 
GCAGATCAGT CAAGCCAGTC TCCGTCCTCT TCGATATCTC CATGTTCAGA AAACAGAGTA 
ACTCAGTTCA TCCGTCAGTA TGTTGCTTCC TGCCTGCTGG TGTCCGACTC CAACACTGAA 
CTCTCTTATG TCCTACCATC AGAGGCTGTC AAGAAAGGCT GCTTTGAGAG ACTCTTCCAG 
GCTCTGGAGC AGAGTTTAGA CAGCTTGGCC TTGACCAGCT TTGGCGTGAT GGACACCACA 
TTGGAGGAGG TGTTTCTAAA GGTGTCAGAA GAAGATCTGT CGCTGGAAAA CAGCGATGCA 
GACATGAAGG ACTCTCCAGG CGGAGCATCA GCCGGGAAGC CGTCAGGTCT GTTAGGAGGG 
CCTCAGTGTG AAGGAGCTCC AGCCGCTGCT GTGATGAGGC CAGAGGTGGA GCTCAGCAAC 
CTGATGATGT GCTCCAGGCT GAGCCCAAGC CAAGCATCCC TGCAGTCCGG CTCTTCTCTG 
GGCTCAGTGA GAGGAGATGA AGGGGGTCCC TATTCTGAGT TCTATGGAGA CTATTGCCCA 
CTGTTTGACA ACGGTCAGGA TCTGGACTCT GCCAGTCTCA GAGATGAAGA GTCATCTCCT 
CAGCCCACTG TACTCGAGGG CCAGGGCAGC TTCAAGCTCG ATGGCTGGTG GCTTAAACTC 
CGCCAGTTTC ATGGGCTGAT CGTGAAAAGG TTTCACTGTG CCAAGAGGAA CACCAAGGGC 
ATTTTCTCTC AGATTCTGCT GCCTGCTTTC TTCGTATGTG TGGCAATGAC TGTGGCTCTG 
TCTGTGCCTG AGATTGGTGA CCTTCCTCCC CTGATCCTGT CTCCATCTCA ATATCACAAC 
TACACACAGC CTCGAGGAAA CTTCATCCCC TACGCCAATG AAGACAGATC TCAATACAGG 
ACTAAGTTTT CTCCAGACGC AGGCCCACAG AGGATTGTTA ACACCCTCCG TCTGCCATCT 
GGAGTCGGTG CGACTTGTAT CCTGAAGACT CCCTTTAACA GCACCCTGGA CCAACTGGCC 
CAAACTCTCA ATCCTTATGC CAACAACTCT AAAACCCTGG CAGCCCGCTA CTTTGACTCT 
ATGTGCCTGG ACTCCTTCAC GCAGGGTGTG CCTTTGTCGA ATTTCGTTCC CCCTCCGCCC 
TCTCCTGCCC CCTCAGACGA CCCGGAGGCT CATTTTGAAG ACGGCCTGTG GAACTACACT 
GCAGCTCCTA CTACTGTCAG AGAGATGGCC ACGTCTCCCC CGACTCTGCC CTTGAGTATC 
CATGAGCCGG TGCGCTGTAT CTGCTCTATG CAGGGCACAG GCTTCTCCTG CCCCAGTGGA 
GTTGGGGGTC GACCTCCTCT GATGAAAGTG GTGACCGGAG ACATCCTGGT GGACATTACA 
GGTCGCAACG TGTCTGAATA TCTCCTGTAC ACGTCTGATC GGCTCAGGTT ACACAGATAT 
GGAGGCTTTA CTGTTGGAAA CATTCAGAAA TCTGTCCCAG CATCCTTCGG GAGAAAAACA 
CCACCTATGG TGCGCAAAAT AGCAGTGAGG CGATCCTCAC AGGTGCTGTA TAATAACAAA 
GGCTATCACA GTATGCCAAC ATATCTAAAT GTCCTCAACA ATGCCATTCT CAGAGCCAAT 
CTGCCCAGTA GTAAAGGAAA CCCTTCTGCA TACGGCATTA CAGTGACCAA TCATCCCATG 
AACAGAACCA GTGCCAGTCT TTCTCTGGAT TATCTGCTCC AGGGCACTGA TGTGGTTATT 
GCCATCTTCA TCATTGTAGC CATGTCTTTC GTGCCTGCGA GCTTTGTGGT CTTTCTGGTA 
GCGGAAAAAT CCACCAAAGC CAAACACCTG CAGTTTGTCA GCGGATGCGA CCCTGTCACG 
TACTGGCTAG CAAACTACAT ATGGGACATG CTGAACTACC TGGTTCCAGC TACATGCTGT 
GTCCTAATTT TGTTTGTGTT CGACCTTCCT GCGTACACGT CACCTACAAA TTTCCCAGCA 
GTGCTCTCTC TGTTTCTGCT TTATGGCTGG TCTATAACCC CCATCATGTA CCCAGCCTCC 
TTCTGGTTTG AGGTGCCCAG CACAGCTTAT GTTTTCCTCA TAGTCATCAA TCTCTTCATT 
GGCATCACAG CCACGGTGGC CACTTTCCTC CTCCAGCTGT TTGAGCGCGA CAAGGACCTG 
AAGTTGGTAA ACAGCTACCT GAAGTCATGT TTTCTCATCT TCCCCAACTA TAACCTGGGT 
CACGGATTGA TGGAGATGGC CTATAATGAG TATATCAATG AATATTACGC CAAGATAGGC 
CAGTTTGATA AAGTGAAGTC TCCATTTGAA TGGGACATTG TGACGCGGGG ACTGGTAGCA 
ATGACCATTG AGGGCTTCGT CGGCTTTCTC ATTACAATTC TGTGCCAGTA CAACTTCCTC 
AGGAAAGCCC AGAGAGTGCC TGTCAACAGT CAGCCAGTGG AGGACGATGA TGTTGATGTG 
GCCTGTGAAA GACGCCGAGT TCTGAGGGGA GACGCTGACA ACGACATGCT GAAGATCGAC 
AACCTAACCA AGGTGTACAA GTCTCGTAAG ATGGGCCGGA TCCTGGCAGT GGACCGATTG 
TGTCTGGGGG TCCGTCCTGG TGAATGCTTC GGTCTGCTGG GAGTGAATGG GGCAGGAAAG 
ACCACCACCT TCAAAATGTT GACAGGAGAT GAGAGCACAA CAGGGGGAGA AGCCTTTATC 
CAGGAACACA GTATCTTGCG GGAGCTGTTA CGTGTGCAGC AGAGCATCGG TTACTGCCCT 
CAGTTTGACG CTCTGTTTGA TGATCTGACG GCTCGAGAGC ATCTGGAGCT CTACACGCGC 
CTCCGCGGCA TCCCATGGAA AGATGAGGAG CGGGTGGTTC AGTGGGCTTT GGAGAAGTTG 
GAGTTGTCCA AGTATGCCGA CAAGCCTGCT GGCACCTACA GCGGAGGCAA CAAACGCAAA 
CTCTCTACAG CCATTGCTCT GATTGGATAC CCATCGCTCA TCTTTTTGGA TGAGCCCACC 
ACAGGTATGG ACCCAAAGGC TCGCCGTTTC TTATGGAACC TAATTCTTGA CATCATCAAG 
ACGGGCCGCT CAGTAGTGTT GACGTCACAC AGTATGGAGG AGTGTGAAGC GCTGTGCACT 
AGACTGGGCA TCATGGTTAA TGGCAGGTTC AAGTGTCTAG GAAGCATCCA GCATCTCAAA 
AACAGATTCG GAGATGGATA CATGATAACA GTACGCACTA AGACCACCGC TAGCGTTAAA 
GAAGTGATTC GGTTTTTTAA CAGAAATTTT CCTGAGGCAA TTCTAAAGGA GCGACACCAC 
ACAAAGATTC AGTACCAGCT GAAGTCAGAG AACATCTCTC TGGCTCAGGT CTTCAGCAAA 
ATGGAGCAGG TGGTGGAGGT GCTGAGCATC GAGGATTATT CCGTCAGCCA AACCACACTG 
GATAACGTCT TTGTTAACTT TGCAAAAAAG CAAAGCGACA ACTTAGAGCA GCAAGAAAGT 
TCTCCATCTA GTGGTGGCCA GTCGCCACTG CAGCATCTGC TCAGCCTCCT CAGGCCTCGT 
CCTGCAAACA CTGAGCTCAA CGCCCTCGTG AGTGAAGAGC CTGAGGAACT GGAGAGTGAT 
GATGACGAAG GACTGATCAG CTTTGAGGAA GAGAGGGTGC AGTTGTCCTT TAATACAGAC 
ACTCTGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165287.1
CDS	554..8065
Translation	MGFLHQLHLLLWKNVTLKRRGPWVLAFEIFIPLVLFFILLGLRQKKPAIPVKEAFYSAAPLTSAGIIPIMQSLCPDGQRDEFGFLQYKNSTVTQLLERISEVVEQNHLFTSDRPSLGEELESLQQHLESLSSAQGPLESHYNTSHGFTVGSVLRNQSMFQQFLMRNLSLPNDTSSLLLSSPVNLKEVYNLIFGTYPKAGSKGGERDEGQHQLNSPGEKLVQLEKQLLGGWHSVDGGLIQKALRDPTKAAHRQALLKVFSQALGIAGGVGGQKFDPQALREMEGVLLTGAVLESLTCGEGGNSEIGKILLVPEKQQAVLQAYRTTVCGLGAGQRAELFRQLSEELRDQIDTQRVFDQLRLEQSNMSGLLDQSRLGALLKNLADVERLLRDVDLLSGLARLLPKGACAGHQPPPQANTTSWSSNSTTWGPNTTDSSVEEGEGASESEAEAENPKSQFSAFVQLWAGLQPILCGNNRIFEPEALKQGNMSSLGFTSKEQRNLGLLVHLMTTNPKILYSPIDSEVDKVIQKANETFAFVGNVTHYARVWLNISAELRAFLEEGKLQNHLMWLQQFITDLKRHHELLNVSDSELMNSLLEENFTLPNTTTLLEQLHTIDNAACGWTNFMSKVSVDIFRGFPDEESIVNYTLNQAYHDNVSVFASVIFQTNKDGSLPPHVMYKIRQNSSFTEKTNEIRRAYWRPGPNTGGKFYFLYGFVWIQDMIERAIINTFVGHDVVEPGNYVQMFPYPCYTRDDFLFVIEHMMPLCMVISWVYSVAMMIQHIVAEKEQRLKEVMKMMGLNNAVHWVAWFITGFVQLSISVTALTAILKYGKVLLHSDPFIIWLFLTIYAVATIMFCFLVSVLYSKAKLASACGGIIYFLSYVPYMYVAIREEVAHDKITAFEKCIASLMSTTAFGLGSKYFALYEVAGVGIQWRTINQSPVEGDDFNLLLSMVMLTIDAIVYGVLTWYIEAVHPGMYGLPRPWYFPLQKSYWLGSGRIETWEWPWGGGTRLSVMEEDQACAMEHRRSEETRGIEEEPNHLPLVVCIDKLTKVYKTGSKLALNKLSLNLHENQVVSFLGHNGAGKTTTMSILTGLFPPTSGSATIYGHDIRTEMERIRQNLGMCPQHNVLFDKLSVEEHLWFYSRLKGMAEEDIRKEMDKMIEDLELSNKRHSLVQTLSGGMKRKLSVAIAFVGGSRAVILDEPTAGVDPYARRAIWDLILKYKQGRTILLSTHHMDEADLLGDRIAIISHGKLKCCGSPLFLKSTYGDGYKLTLVKKQSDSHTADQSSQSPSSSISPCSENRVTQFIRQYVASCLLVSDSNTELSYVLPSEAVKKGCFERLFQALEQSLDSLALTSFGVMDTTLEEVFLKVSEEDLSLENSDADMKDSPGGASAGKPSGLLGGPQCEGAPAAAVMRPEVELSNLMMCSRLSPSQASLQSGSSLGSVRGDEGGPYSEFYGDYCPLFDNGQDLDSASLRDEESSPQPTVLEGQGSFKLDGWWLKLRQFHGLIVKRFHCAKRNTKGIFSQILLPAFFVCVAMTVALSVPEIGDLPPLILSPSQYHNYTQPRGNFIPYANEDRSQYRTKFSPDAGPQRIVNTLRLPSGVGATCILKTPFNSTLDQLAQTLNPYANNSKTLAARYFDSMCLDSFTQGVPLSNFVPPPPSPAPSDDPEAHFEDGLWNYTAAPTTVREMATSPPTLPLSIHEPVRCICSMQGTGFSCPSGVGGRPPLMKVVTGDILVDITGRNVSEYLLYTSDRLRLHRYGGFTVGNIQKSVPASFGRKTPPMVRKIAVRRSSQVLYNNKGYHSMPTYLNVLNNAILRANLPSSKGNPSAYGITVTNHPMNRTSASLSLDYLLQGTDVVIAIFIIVAMSFVPASFVVFLVAEKSTKAKHLQFVSGCDPVTYWLANYIWDMLNYLVPATCCVLILFVFDLPAYTSPTNFPAVLSLFLLYGWSITPIMYPASFWFEVPSTAYVFLIVINLFIGITATVATFLLQLFERDKDLKLVNSYLKSCFLIFPNYNLGHGLMEMAYNEYINEYYAKIGQFDKVKSPFEWDIVTRGLVAMTIEGFVGFLITILCQYNFLRKAQRVPVNSQPVEDDDVDVACERRRVLRGDADNDMLKIDNLTKVYKSRKMGRILAVDRLCLGVRPGECFGLLGVNGAGKTTTFKMLTGDESTTGGEAFIQEHSILRELLRVQQSIGYCPQFDALFDDLTAREHLELYTRLRGIPWKDEERVVQWALEKLELSKYADKPAGTYSGGNKRKLSTAIALIGYPSLIFLDEPTTGMDPKARRFLWNLILDIIKTGRSVVLTSHSMEECEALCTRLGIMVNGRFKCLGSIQHLKNRFGDGYMITVRTKTTASVKEVIRFFNRNFPEAILKERHHTKIQYQLKSENISLAQVFSKMEQVVEVLSIEDYSVSQTTLDNVFVNFAKKQSDNLEQQESSPSSGGQSPLQHLLSLLRPRPANTELNALVSEEPEELESDDDEGLISFEEERVQLSFNTDTLC

Target ORF information:

RefSeq Version	XM_005165230.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165230.4

ORF Insert Sequence:

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG CCTTCTATAG CGCGGCGCCG 
CTCACCTCGG CGGGCATCAT TCCCATCATG CAGTCGTTGT GTCCGGATGG TCAGCGGGAT 
GAGTTTGGCT TCCTGCAGTA TAAAAACTCC ACTGTGACTC AATTGCTGGA AAGGATAAGT 
GAAGTTGTGG AGCAGAATCA CTTGTTTACA TCAGACAGGC CCAGTCTGGG AGAGGAGCTG 
GAGTCTCTGC AGCAGCACCT CGAAAGTCTG AGCTCAGCAC AAGGCCCTCT AGAGAGCCAC 
TACAACACCA GCCACGGTTT CACTGTGGGA AGCGTCCTCA GGAACCAGTC GATGTTCCAG 
CAGTTTCTGA TGCGAAATCT CTCCCTGCCG AACGACACGT CCAGCCTGCT GCTCTCCTCC 
CCCGTCAATC TAAAAGAGGT ATATAATCTC ATCTTTGGGA CGTATCCCAA AGCGGGATCA 
AAGGGAGGAG AAAGAGATGA AGGACAGCAC CAACTCAACA GTCCTGGAGA GAAGCTTGTA 
CAGCTGGAGA AGCAACTACT TGGAGGCTGG CACAGTGTAG ACGGGGGTCT GATTCAGAAG 
GCACTGAGAG ATCCCACAAA AGCTGCTCAC CGTCAAGCTC TTCTCAAAGT CTTCTCTCAG 
GCGTTGGGCA TCGCTGGCGG TGTAGGAGGT CAAAAATTCG ATCCTCAAGC ACTGCGTGAG 
ATGGAGGGAG TTCTGTTGAC TGGTGCAGTG CTTGAAAGCC TTACCTGTGG GGAAGGAGGG 
AACAGTGAGA TTGGCAAAAT CCTATTGGTC CCTGAGAAAC AACAGGCGGT TTTACAGGCT 
TACCGCACGA CTGTTTGTGG TTTAGGGGCG GGGCAGAGGG CGGAGCTTTT CAGACAGTTG 
AGTGAAGAAC TGAGAGATCA GATTGACACA CAGAGAGTCT TTGATCAGTT GCGTCTTGAG 
CAGTCTAATA TGTCTGGTCT GCTGGATCAG TCCCGTCTCG GGGCTCTCCT GAAGAACCTG 
GCTGATGTGG AGCGGCTGCT GAGGGACGTG GATCTACTGT CAGGTCTGGC CAGACTGCTG 
CCGAAAGGGG CCTGTGCGGG TCACCAGCCT CCTCCTCAGG CCAACACCAC CTCCTGGAGC 
TCCAACTCTA CCACATGGGG TCCCAATACA ACTGACAGTT CTGTGGAGGA AGGAGAAGGA 
GCTTCTGAGA GCGAGGCAGA GGCTGAAAAC CCCAAATCAC AGTTTTCTGC ATTTGTTCAG 
CTGTGGGCAG GACTTCAACC CATACTTTGT GGGAATAATA GGATTTTCGA ACCAGAAGCG 
CTGAAACAAG GAAATATGAG TTCTCTGGGC TTCACCAGCA AAGAACAGAG AAATTTAGGA 
TTACTTGTTC ATCTCATGAC TACAAATCCC AAAATCCTCT ACTCTCCCAT TGACTCAGAG 
GTGGACAAGG TCATACAAAA GGCCAATGAG ACCTTTGCTT TTGTGGGGAA TGTGACTCAC 
TATGCCCGTG TGTGGCTCAA CATCTCTGCT GAATTAAGGG CCTTTTTAGA GGAGGGTAAA 
TTGCAAAATC ACCTGATGTG GCTCCAGCAG TTTATTACCG ACCTGAAGAG GCACCATGAG 
CTGCTGAACG TATCAGACAG TGAGCTGATG AACAGTCTGC TGGAGGAGAA CTTCACCCTG 
CCAAACACAA CCACCTTACT GGAGCAGCTG CACACCATTG ATAATGCTGC TTGTGGATGG 
ACTAACTTCA TGTCAAAGGT CAGTGTGGAT ATCTTCAGAG GCTTCCCTGA TGAAGAAAGC 
ATTGTCAATT ACACACTGAA CCAGGCCTAC CACGACAACG TCTCAGTCTT TGCAAGTGTG 
ATTTTCCAGA CCAATAAAGA TGGATCCCTG CCACCCCATG TTATGTATAA AATTCGACAG 
AACTCGAGCT TTACCGAGAA AACCAATGAA ATCCGACGTG CTTATTGGCG CCCAGGGCCA 
AACACAGGCG GCAAATTCTA CTTCCTTTAT GGCTTTGTGT GGATCCAAGA TATGATTGAA 
CGGGCAATCA TCAACACCTT TGTTGGCCAC GATGTTGTGG AGCCTGGAAA TTACGTGCAG 
ATGTTTCCTT ATCCGTGCTA CACCAGAGAC GATTTTCTCT TTGTTATCGA GCACATGATG 
CCTCTGTGTA TGGTCATATC CTGGGTCTAC TCTGTGGCCA TGATGATCCA GCACATCGTA 
GCAGAGAAGG AGCAAAGACT GAAAGAGGTA ATGAAGATGA TGGGCTTGAA TAACGCAGTG 
CACTGGGTGG CCTGGTTCAT CACAGGCTTC GTGCAGCTTT CCATCTCAGT GACGGCCCTC 
ACTGCCATAC TGAAATACGG GAAAGTCCTC CTTCACAGCG ATCCCTTCAT CATATGGCTT 
TTTCTTACAA TCTACGCTGT CGCAACCATC ATGTTCTGTT TCCTGGTGTC GGTGTTGTAC 
TCTAAAGCCA AACTGGCCTC GGCATGTGGA GGAATCATCT ACTTCCTGAG TTATGTGCCC 
TACATGTATG TGGCCATAAG AGAAGAGGTG GCTCACGATA AGATCACTGC CTTTGAGAAG 
TGCATAGCGT CTTTGATGTC TACCACAGCG TTCGGTCTGG GATCCAAGTA CTTTGCCCTG 
TATGAAGTGG CTGGCGTGGG AATTCAGTGG CGCACTATTA ACCAGTCACC TGTAGAGGGC 
GATGACTTCA ATCTACTGCT GTCTATGGTC ATGCTCACTA TTGACGCCAT TGTATATGGT 
GTGTTGACCT GGTATATAGA AGCCGTCCAT CCAGGGATGT ATGGACTGCC TCGGCCATGG 
TATTTCCCCT TGCAGAAGTC CTATTGGTTA GGTAGTGGCC GCATAGAGAC GTGGGAGTGG 
CCGTGGGGTG GAGGCACCAG GCTGAGTGTT ATGGAAGAGG ACCAGGCTTG TGCCATGGAG 
CATAGACGCT CAGAGGAAAC CCGTGGAATT GAAGAAGAGC CCAATCATTT GCCGCTGGTG 
GTGTGCATCG ACAAACTGAC CAAAGTCTAC AAGACGGGCA GCAAACTGGC TCTAAACAAG 
CTGAGCCTGA ACCTGCATGA GAACCAGGTT GTCTCTTTCT TGGGACACAA TGGGGCTGGC 
AAGACCACCA CTATGTCCAT TCTCACGGGA CTCTTTCCAC CCACCTCTGG CTCTGCCACC 
ATATACGGCC ATGACATCCG CACAGAGATG GAGCGCATCA GGCAGAACTT GGGCATGTGT 
CCCCAGCACA ACGTCCTGTT TGATAAGCTC AGTGTGGAGG AACATCTGTG GTTCTACTCA 
CGGCTGAAAG GCATGGCAGA GGAAGATATT CGCAAGGAGA TGGACAAGAT GATAGAGGAT 
CTCGAATTAT CTAATAAACG TCACAGTCTG GTTCAAACCC TGTCAGGAGG GATGAAAAGG 
AAGCTGTCAG TGGCCATTGC TTTTGTAGGT GGGTCTCGGG CAGTGATTTT GGATGAACCA 
ACAGCAGGAG TAGACCCATA TGCCCGTCGA GCGATCTGGG ACCTCATCCT GAAATATAAG 
CAAGGTCGCA CCATCCTGCT GTCCACTCAC CACATGGATG AGGCTGATCT GCTGGGTGAC 
CGCATTGCCA TCATCTCTCA TGGCAAGCTC AAGTGCTGCG GTTCTCCTCT GTTCCTCAAG 
AGCACATATG GAGACGGATA CAAACTCACA CTTGTTAAAA AACAGAGTGA CTCACACACT 
GCAGATCAGT CAAGCCAGTC TCCGTCCTCT TCGATATCTC CATGTTCAGA AAACAGAGTA 
ACTCAGTTCA TCCGTCAGTA TGTTGCTTCC TGCCTGCTGG TGTCCGACTC CAACACTGAA 
CTCTCTTATG TCCTACCATC AGAGGCTGTC AAGAAAGGCT GCTTTGAGAG ACTCTTCCAG 
GCTCTGGAGC AGAGTTTAGA CAGCTTGGCC TTGACCAGCT TTGGCGTGAT GGACACCACA 
TTGGAGGAGG TGTTTCTAAA GGTGTCAGAA GAAGATCTGT CGCTGGAAAA CAGCGATGCA 
GACATGAAGG ACTCTCCAGG CGGAGCATCA GCCGGGAAGC CGTCAGGTCT GTTAGGAGGG 
CCTCAGTGTG AAGGAGCTCC AGCCGCTGCT GTGATGAGGC CAGAGGTGGA GCTCAGCAAC 
CTGATGATGT GCTCCAGGCT GAGCCCAAGC CAAGCATCCC TGCAGTCCGG CTCTTCTCTG 
GGCTCAGTGA GAGGAGATGA AGGGGGTCCC TATTCTGAGT TCTATGGAGA CTATTGCCCA 
CTGTTTGACA ACGGTCAGGA TCTGGACTCT GCCAGTCTCA GAGATGAAGA GTCATCTCCT 
CAGCCCACTG TACTCGAGGG CCAGGGCAGC TTCAAGCTCG ATGGCTGGTG GCTTAAACTC 
CGCCAGTTTC ATGGGCTGAT CGTGAAAAGG TTTCACTGTG CCAAGAGGAA CACCAAGGGC 
ATTTTCTCTC AGATTCTGCT GCCTGCTTTC TTCGTATGTG TGGCAATGAC TGTGGCTCTG 
TCTGTGCCTG AGATTGGTGA CCTTCCTCCC CTGATCCTGT CTCCATCTCA ATATCACAAC 
TACACACAGC CTCGAGGAAA CTTCATCCCC TACGCCAATG AAGACAGATC TCAATACAGG 
ACTAAGTTTT CTCCAGACGC AGGCCCACAG AGGATTGTTA ACACCCTCCG TCTGCCATCT 
GGAGTCGGTG CGACTTGTAT CCTGAAGACT CCCTTTAACA GCACCCTGGA CCAACTGGCC 
CAAACTCTCA ATCCTTATGC CAACAACTCT AAAACCCTGG CAGCCCGCTA CTTTGACTCT 
ATGTGCCTGG ACTCCTTCAC GCAGGGTGTG CCTTTGTCGA ATTTCGTTCC CCCTCCGCCC 
TCTCCTGCCC CCTCAGACGA CCCGGAGGCT CATTTTGAAG ACGGCCTGTG GAACTACACT 
GCAGCTCCTA CTACTGTCAG AGAGATGGCC ACGTCTCCCC CGACTCTGCC CTTGAGTATC 
CATGAGCCGG TGCGCTGTAT CTGCTCTATG CAGGGCACAG GCTTCTCCTG CCCCAGTGGA 
GTTGGGGGTC GACCTCCTCT GATGAAAGTG GTGACCGGAG ACATCCTGGT GGACATTACA 
GGTCGCAACG TGTCTGAATA TCTCCTGTAC ACGTCTGATC GGCTCAGGTT ACACAGATAT 
GGAGGCTTTA CTGTTGGAAA CATTCAGAAA TCTGTCCCAG CATCCTTCGG GAGAAAAACA 
CCACCTATGG TGCGCAAAAT AGCAGTGAGG CGATCCTCAC AGGTGCTGTA TAATAACAAA 
GGCTATCACA GTATGCCAAC ATATCTAAAT GTCCTCAACA ATGCCATTCT CAGAGCCAAT 
CTGCCCAGTA GTAAAGGAAA CCCTTCTGCA TACGGCATTA CAGTGACCAA TCATCCCATG 
AACAGAACCA GTGCCAGTCT TTCTCTGGAT TATCTGCTCC AGGGCACTGA TGTGGTTATT 
GCCATCTTCA TCATTGTAGC CATGTCTTTC GTGCCTGCGA GCTTTGTGGT CTTTCTGGTA 
GCGGAAAAAT CCACCAAAGC CAAACACCTG CAGTTTGTCA GCGGATGCGA CCCTGTCACG 
TACTGGCTAG CAAACTACAT ATGGGACATG CTGAACTACC TGGTTCCAGC TACATGCTGT 
GTCCTAATTT TGTTTGTGTT CGACCTTCCT GCGTACACGT CACCTACAAA TTTCCCAGCA 
GTGCTCTCTC TGTTTCTGCT TTATGGCTGG TCTATAACCC CCATCATGTA CCCAGCCTCC 
TTCTGGTTTG AGGTGCCCAG CACAGCTTAT GTTTTCCTCA TAGTCATCAA TCTCTTCATT 
GGCATCACAG CCACGGTGGC CACTTTCCTC CTCCAGCTGT TTGAGCGCGA CAAGGACCTG 
AAGTTGGTAA ACAGCTACCT GAAGTCATGT TTTCTCATCT TCCCCAACTA TAACCTGGGT 
CACGGATTGA TGGAGATGGC CTATAATGAG TATATCAATG AATATTACGC CAAGATAGGC 
CAGTTTGATA AAGTGAAGTC TCCATTTGAA TGGGACATTG TGACGCGGGG ACTGGTAGCA 
ATGACCATTG AGGGCTTCGT CGGCTTTCTC ATTACAATTC TGTGCCAGTA CAACTTCCTC 
AGGAAAGCCC AGAGAGTGCC TGTCAACAGT CAGCCAGTGG AGGACGATGA TGTTGATGTG 
GCCTGTGAAA GACGCCGAGT TCTGAGGGGA GACGCTGACA ACGACATGCT GAAGATCGAC 
AACCTAACCA AGGTGTACAA GTCTCGTAAG ATGGGCCGGA TCCTGGCAGT GGACCGATTG 
TGTCTGGGGG TCCGTCCTGG TGAATGCTTC GGTCTGCTGG GAGTGAATGG GGCAGGAAAG 
ACCACCACCT TCAAAATGTT GACAGGAGAT GAGAGCACAA CAGGGGGAGA AGCCTTTATC 
CAGGAACACA GTATCTTGCG GGAGCTGTTA CGTGTGCAGC AGAGCATCGG TTACTGCCCT 
CAGTTTGACG CTCTGTTTGA TGATCTGACG GCTCGAGAGC ATCTGGAGCT CTACACGCGC 
CTCCGCGGCA TCCCATGGAA AGATGAGGAG CGGGTGGTTC AGTGGGCTTT GGAGAAGTTG 
GAGTTGTCCA AGTATGCCGA CAAGCCTGCT GGCACCTACA GCGGAGGCAA CAAACGCAAA 
CTCTCTACAG CCATTGCTCT GATTGGATAC CCATCGCTCA TCTTTTTGGA TGAGCCCACC 
ACAGGTATGG ACCCAAAGGC TCGCCGTTTC TTATGGAACC TAATTCTTGA CATCATCAAG 
ACGGGCCGCT CAGTAGTGTT GACGTCACAC AGTATGGAGG AGTGTGAAGC GCTGTGCACT 
AGACTGGGCA TCATGGTTAA TGGCAGGTTC AAGTGTCTAG GAAGCATCCA GCATCTCAAA 
AACAGATTCG GAGATGGATA CATGATAACA GTACGCACTA AGACCACCGC TAGCGTTAAA 
GAAGTGATTC GGTTTTTTAA CAGAAATTTT CCTGAGGCAA TTCTAAAGGA GCGACACCAC 
ACAAAGATTC AGTACCAGCT GAAGTCAGAG AACATCTCTC TGGCTCAGGT CTTCAGCAAA 
ATGGAGCAGG TGGTGGAGGT GCTGAGCATC GAGGATTATT CCGTCAGCCA AACCACACTG 
GATAACGTCT TTGTTAACTT TGCAAAAAAG CAAAGCGACA ACTTAGAGCA GCAAGAAAGT 
TCTCCATCTA GTGGTGGCCA GTCGCCACTG CAGCATCTGC TCAGCCTCCT CAGGCCTCGT 
CCTGCAAACA CTGAGCTCAA CGCCCTCGTG AGTGAAGAGC CTGAGGAACT GGAGAGTGAT 
GATGACGAAG GACTGATCAG CTTTGAGGAA GAGAGGGTGC AGTTGTCCTT TAATACAGAC 
ACTCTGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

abca2 ( XM_005165230.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165287.1 Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X2, mRNA.

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CloneID	ODa10382
Clone ID Related Accession (Same CDS sequence)	XM_005165230.4 , XM_005165230.3
Accession Version	XM_005165230.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ATP-binding cassette sub-family A member 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165230.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG CCTTCTATAG CGCGGCGCCG 
CTCACCTCGG CGGGCATCAT TCCCATCATG CAGTCGTTGT GTCCGGATGG TCAGCGGGAT 
GAGTTTGGCT TCCTGCAGTA TAAAAACTCC ACTGTGACTC AATTGCTGGA AAGGATAAGT 
GAAGTTGTGG AGCAGAATCA CTTGTTTACA TCAGACAGGC CCAGTCTGGG AGAGGAGCTG 
GAGTCTCTGC AGCAGCACCT CGAAAGTCTG AGCTCAGCAC AAGGCCCTCT AGAGAGCCAC 
TACAACACCA GCCACGGTTT CACTGTGGGA AGCGTCCTCA GGAACCAGTC GATGTTCCAG 
CAGTTTCTGA TGCGAAATCT CTCCCTGCCG AACGACACGT CCAGCCTGCT GCTCTCCTCC 
CCCGTCAATC TAAAAGAGGT ATATAATCTC ATCTTTGGGA CGTATCCCAA AGCGGGATCA 
AAGGGAGGAG AAAGAGATGA AGGACAGCAC CAACTCAACA GTCCTGGAGA GAAGCTTGTA 
CAGCTGGAGA AGCAACTACT TGGAGGCTGG CACAGTGTAG ACGGGGGTCT GATTCAGAAG 
GCACTGAGAG ATCCCACAAA AGCTGCTCAC CGTCAAGCTC TTCTCAAAGT CTTCTCTCAG 
GCGTTGGGCA TCGCTGGCGG TGTAGGAGGT CAAAAATTCG ATCCTCAAGC ACTGCGTGAG 
ATGGAGGGAG TTCTGTTGAC TGGTGCAGTG CTTGAAAGCC TTACCTGTGG GGAAGGAGGG 
AACAGTGAGA TTGGCAAAAT CCTATTGGTC CCTGAGAAAC AACAGGCGGT TTTACAGGCT 
TACCGCACGA CTGTTTGTGG TTTAGGGGCG GGGCAGAGGG CGGAGCTTTT CAGACAGTTG 
AGTGAAGAAC TGAGAGATCA GATTGACACA CAGAGAGTCT TTGATCAGTT GCGTCTTGAG 
CAGTCTAATA TGTCTGGTCT GCTGGATCAG TCCCGTCTCG GGGCTCTCCT GAAGAACCTG 
GCTGATGTGG AGCGGCTGCT GAGGGACGTG GATCTACTGT CAGGTCTGGC CAGACTGCTG 
CCGAAAGGGG CCTGTGCGGG TCACCAGCCT CCTCCTCAGG CCAACACCAC CTCCTGGAGC 
TCCAACTCTA CCACATGGGG TCCCAATACA ACTGACAGTT CTGTGGAGGA AGGAGAAGGA 
GCTTCTGAGA GCGAGGCAGA GGCTGAAAAC CCCAAATCAC AGTTTTCTGC ATTTGTTCAG 
CTGTGGGCAG GACTTCAACC CATACTTTGT GGGAATAATA GGATTTTCGA ACCAGAAGCG 
CTGAAACAAG GAAATATGAG TTCTCTGGGC TTCACCAGCA AAGAACAGAG AAATTTAGGA 
TTACTTGTTC ATCTCATGAC TACAAATCCC AAAATCCTCT ACTCTCCCAT TGACTCAGAG 
GTGGACAAGG TCATACAAAA GGCCAATGAG ACCTTTGCTT TTGTGGGGAA TGTGACTCAC 
TATGCCCGTG TGTGGCTCAA CATCTCTGCT GAATTAAGGG CCTTTTTAGA GGAGGGTAAA 
TTGCAAAATC ACCTGATGTG GCTCCAGCAG TTTATTACCG ACCTGAAGAG GCACCATGAG 
CTGCTGAACG TATCAGACAG TGAGCTGATG AACAGTCTGC TGGAGGAGAA CTTCACCCTG 
CCAAACACAA CCACCTTACT GGAGCAGCTG CACACCATTG ATAATGCTGC TTGTGGATGG 
ACTAACTTCA TGTCAAAGGT CAGTGTGGAT ATCTTCAGAG GCTTCCCTGA TGAAGAAAGC 
ATTGTCAATT ACACACTGAA CCAGGCCTAC CACGACAACG TCTCAGTCTT TGCAAGTGTG 
ATTTTCCAGA CCAATAAAGA TGGATCCCTG CCACCCCATG TTATGTATAA AATTCGACAG 
AACTCGAGCT TTACCGAGAA AACCAATGAA ATCCGACGTG CTTATTGGCG CCCAGGGCCA 
AACACAGGCG GCAAATTCTA CTTCCTTTAT GGCTTTGTGT GGATCCAAGA TATGATTGAA 
CGGGCAATCA TCAACACCTT TGTTGGCCAC GATGTTGTGG AGCCTGGAAA TTACGTGCAG 
ATGTTTCCTT ATCCGTGCTA CACCAGAGAC GATTTTCTCT TTGTTATCGA GCACATGATG 
CCTCTGTGTA TGGTCATATC CTGGGTCTAC TCTGTGGCCA TGATGATCCA GCACATCGTA 
GCAGAGAAGG AGCAAAGACT GAAAGAGGTA ATGAAGATGA TGGGCTTGAA TAACGCAGTG 
CACTGGGTGG CCTGGTTCAT CACAGGCTTC GTGCAGCTTT CCATCTCAGT GACGGCCCTC 
ACTGCCATAC TGAAATACGG GAAAGTCCTC CTTCACAGCG ATCCCTTCAT CATATGGCTT 
TTTCTTACAA TCTACGCTGT CGCAACCATC ATGTTCTGTT TCCTGGTGTC GGTGTTGTAC 
TCTAAAGCCA AACTGGCCTC GGCATGTGGA GGAATCATCT ACTTCCTGAG TTATGTGCCC 
TACATGTATG TGGCCATAAG AGAAGAGGTG GCTCACGATA AGATCACTGC CTTTGAGAAG 
TGCATAGCGT CTTTGATGTC TACCACAGCG TTCGGTCTGG GATCCAAGTA CTTTGCCCTG 
TATGAAGTGG CTGGCGTGGG AATTCAGTGG CGCACTATTA ACCAGTCACC TGTAGAGGGC 
GATGACTTCA ATCTACTGCT GTCTATGGTC ATGCTCACTA TTGACGCCAT TGTATATGGT 
GTGTTGACCT GGTATATAGA AGCCGTCCAT CCAGGGATGT ATGGACTGCC TCGGCCATGG 
TATTTCCCCT TGCAGAAGTC CTATTGGTTA GGTAGTGGCC GCATAGAGAC GTGGGAGTGG 
CCGTGGGGTG GAGGCACCAG GCTGAGTGTT ATGGAAGAGG ACCAGGCTTG TGCCATGGAG 
CATAGACGCT CAGAGGAAAC CCGTGGAATT GAAGAAGAGC CCAATCATTT GCCGCTGGTG 
GTGTGCATCG ACAAACTGAC CAAAGTCTAC AAGACGGGCA GCAAACTGGC TCTAAACAAG 
CTGAGCCTGA ACCTGCATGA GAACCAGGTT GTCTCTTTCT TGGGACACAA TGGGGCTGGC 
AAGACCACCA CTATGTCCAT TCTCACGGGA CTCTTTCCAC CCACCTCTGG CTCTGCCACC 
ATATACGGCC ATGACATCCG CACAGAGATG GAGCGCATCA GGCAGAACTT GGGCATGTGT 
CCCCAGCACA ACGTCCTGTT TGATAAGCTC AGTGTGGAGG AACATCTGTG GTTCTACTCA 
CGGCTGAAAG GCATGGCAGA GGAAGATATT CGCAAGGAGA TGGACAAGAT GATAGAGGAT 
CTCGAATTAT CTAATAAACG TCACAGTCTG GTTCAAACCC TGTCAGGAGG GATGAAAAGG 
AAGCTGTCAG TGGCCATTGC TTTTGTAGGT GGGTCTCGGG CAGTGATTTT GGATGAACCA 
ACAGCAGGAG TAGACCCATA TGCCCGTCGA GCGATCTGGG ACCTCATCCT GAAATATAAG 
CAAGGTCGCA CCATCCTGCT GTCCACTCAC CACATGGATG AGGCTGATCT GCTGGGTGAC 
CGCATTGCCA TCATCTCTCA TGGCAAGCTC AAGTGCTGCG GTTCTCCTCT GTTCCTCAAG 
AGCACATATG GAGACGGATA CAAACTCACA CTTGTTAAAA AACAGAGTGA CTCACACACT 
GCAGATCAGT CAAGCCAGTC TCCGTCCTCT TCGATATCTC CATGTTCAGA AAACAGAGTA 
ACTCAGTTCA TCCGTCAGTA TGTTGCTTCC TGCCTGCTGG TGTCCGACTC CAACACTGAA 
CTCTCTTATG TCCTACCATC AGAGGCTGTC AAGAAAGGCT GCTTTGAGAG ACTCTTCCAG 
GCTCTGGAGC AGAGTTTAGA CAGCTTGGCC TTGACCAGCT TTGGCGTGAT GGACACCACA 
TTGGAGGAGG TGTTTCTAAA GGTGTCAGAA GAAGATCTGT CGCTGGAAAA CAGCGATGCA 
GACATGAAGG ACTCTCCAGG CGGAGCATCA GCCGGGAAGC CGTCAGGTCT GTTAGGAGGG 
CCTCAGTGTG AAGGAGCTCC AGCCGCTGCT GTGATGAGGC CAGAGGTGGA GCTCAGCAAC 
CTGATGATGT GCTCCAGGCT GAGCCCAAGC CAAGCATCCC TGCAGTCCGG CTCTTCTCTG 
GGCTCAGTGA GAGGAGATGA AGGGGGTCCC TATTCTGAGT TCTATGGAGA CTATTGCCCA 
CTGTTTGACA ACGGTCAGGA TCTGGACTCT GCCAGTCTCA GAGATGAAGA GTCATCTCCT 
CAGCCCACTG TACTCGAGGG CCAGGGCAGC TTCAAGCTCG ATGGCTGGTG GCTTAAACTC 
CGCCAGTTTC ATGGGCTGAT CGTGAAAAGG TTTCACTGTG CCAAGAGGAA CACCAAGGGC 
ATTTTCTCTC AGATTCTGCT GCCTGCTTTC TTCGTATGTG TGGCAATGAC TGTGGCTCTG 
TCTGTGCCTG AGATTGGTGA CCTTCCTCCC CTGATCCTGT CTCCATCTCA ATATCACAAC 
TACACACAGC CTCGAGGAAA CTTCATCCCC TACGCCAATG AAGACAGATC TCAATACAGG 
ACTAAGTTTT CTCCAGACGC AGGCCCACAG AGGATTGTTA ACACCCTCCG TCTGCCATCT 
GGAGTCGGTG CGACTTGTAT CCTGAAGACT CCCTTTAACA GCACCCTGGA CCAACTGGCC 
CAAACTCTCA ATCCTTATGC CAACAACTCT AAAACCCTGG CAGCCCGCTA CTTTGACTCT 
ATGTGCCTGG ACTCCTTCAC GCAGGGTGTG CCTTTGTCGA ATTTCGTTCC CCCTCCGCCC 
TCTCCTGCCC CCTCAGACGA CCCGGAGGCT CATTTTGAAG ACGGCCTGTG GAACTACACT 
GCAGCTCCTA CTACTGTCAG AGAGATGGCC ACGTCTCCCC CGACTCTGCC CTTGAGTATC 
CATGAGCCGG TGCGCTGTAT CTGCTCTATG CAGGGCACAG GCTTCTCCTG CCCCAGTGGA 
GTTGGGGGTC GACCTCCTCT GATGAAAGTG GTGACCGGAG ACATCCTGGT GGACATTACA 
GGTCGCAACG TGTCTGAATA TCTCCTGTAC ACGTCTGATC GGCTCAGGTT ACACAGATAT 
GGAGGCTTTA CTGTTGGAAA CATTCAGAAA TCTGTCCCAG CATCCTTCGG GAGAAAAACA 
CCACCTATGG TGCGCAAAAT AGCAGTGAGG CGATCCTCAC AGGTGCTGTA TAATAACAAA 
GGCTATCACA GTATGCCAAC ATATCTAAAT GTCCTCAACA ATGCCATTCT CAGAGCCAAT 
CTGCCCAGTA GTAAAGGAAA CCCTTCTGCA TACGGCATTA CAGTGACCAA TCATCCCATG 
AACAGAACCA GTGCCAGTCT TTCTCTGGAT TATCTGCTCC AGGGCACTGA TGTGGTTATT 
GCCATCTTCA TCATTGTAGC CATGTCTTTC GTGCCTGCGA GCTTTGTGGT CTTTCTGGTA 
GCGGAAAAAT CCACCAAAGC CAAACACCTG CAGTTTGTCA GCGGATGCGA CCCTGTCACG 
TACTGGCTAG CAAACTACAT ATGGGACATG CTGAACTACC TGGTTCCAGC TACATGCTGT 
GTCCTAATTT TGTTTGTGTT CGACCTTCCT GCGTACACGT CACCTACAAA TTTCCCAGCA 
GTGCTCTCTC TGTTTCTGCT TTATGGCTGG TCTATAACCC CCATCATGTA CCCAGCCTCC 
TTCTGGTTTG AGGTGCCCAG CACAGCTTAT GTTTTCCTCA TAGTCATCAA TCTCTTCATT 
GGCATCACAG CCACGGTGGC CACTTTCCTC CTCCAGCTGT TTGAGCGCGA CAAGGACCTG 
AAGTTGGTAA ACAGCTACCT GAAGTCATGT TTTCTCATCT TCCCCAACTA TAACCTGGGT 
CACGGATTGA TGGAGATGGC CTATAATGAG TATATCAATG AATATTACGC CAAGATAGGC 
CAGTTTGATA AAGTGAAGTC TCCATTTGAA TGGGACATTG TGACGCGGGG ACTGGTAGCA 
ATGACCATTG AGGGCTTCGT CGGCTTTCTC ATTACAATTC TGTGCCAGTA CAACTTCCTC 
AGGAAAGCCC AGAGAGTGCC TGTCAACAGT CAGCCAGTGG AGGACGATGA TGTTGATGTG 
GCCTGTGAAA GACGCCGAGT TCTGAGGGGA GACGCTGACA ACGACATGCT GAAGATCGAC 
AACCTAACCA AGGTGTACAA GTCTCGTAAG ATGGGCCGGA TCCTGGCAGT GGACCGATTG 
TGTCTGGGGG TCCGTCCTGG TGAATGCTTC GGTCTGCTGG GAGTGAATGG GGCAGGAAAG 
ACCACCACCT TCAAAATGTT GACAGGAGAT GAGAGCACAA CAGGGGGAGA AGCCTTTATC 
CAGGAACACA GTATCTTGCG GGAGCTGTTA CGTGTGCAGC AGAGCATCGG TTACTGCCCT 
CAGTTTGACG CTCTGTTTGA TGATCTGACG GCTCGAGAGC ATCTGGAGCT CTACACGCGC 
CTCCGCGGCA TCCCATGGAA AGATGAGGAG CGGGTGGTTC AGTGGGCTTT GGAGAAGTTG 
GAGTTGTCCA AGTATGCCGA CAAGCCTGCT GGCACCTACA GCGGAGGCAA CAAACGCAAA 
CTCTCTACAG CCATTGCTCT GATTGGATAC CCATCGCTCA TCTTTTTGGA TGAGCCCACC 
ACAGGTATGG ACCCAAAGGC TCGCCGTTTC TTATGGAACC TAATTCTTGA CATCATCAAG 
ACGGGCCGCT CAGTAGTGTT GACGTCACAC AGTATGGAGG AGTGTGAAGC GCTGTGCACT 
AGACTGGGCA TCATGGTTAA TGGCAGGTTC AAGTGTCTAG GAAGCATCCA GCATCTCAAA 
AACAGATTCG GAGATGGATA CATGATAACA GTACGCACTA AGACCACCGC TAGCGTTAAA 
GAAGTGATTC GGTTTTTTAA CAGAAATTTT CCTGAGGCAA TTCTAAAGGA GCGACACCAC 
ACAAAGATTC AGTACCAGCT GAAGTCAGAG AACATCTCTC TGGCTCAGGT CTTCAGCAAA 
ATGGAGCAGG TGGTGGAGGT GCTGAGCATC GAGGATTATT CCGTCAGCCA AACCACACTG 
GATAACGTCT TTGTTAACTT TGCAAAAAAG CAAAGCGACA ACTTAGAGCA GCAAGAAAGT 
TCTCCATCTA GTGGTGGCCA GTCGCCACTG CAGCATCTGC TCAGCCTCCT CAGGCCTCGT 
CCTGCAAACA CTGAGCTCAA CGCCCTCGTG AGTGAAGAGC CTGAGGAACT GGAGAGTGAT 
GATGACGAAG GACTGATCAG CTTTGAGGAA GAGAGGGTGC AGTTGTCCTT TAATACAGAC 
ACTCTGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165287.1
CDS	552..8063
Translation	MGFLHQLHLLLWKNVTLKRRGPWVLAFEIFIPLVLFFILLGLRQKKPAIPVKEAFYSAAPLTSAGIIPIMQSLCPDGQRDEFGFLQYKNSTVTQLLERISEVVEQNHLFTSDRPSLGEELESLQQHLESLSSAQGPLESHYNTSHGFTVGSVLRNQSMFQQFLMRNLSLPNDTSSLLLSSPVNLKEVYNLIFGTYPKAGSKGGERDEGQHQLNSPGEKLVQLEKQLLGGWHSVDGGLIQKALRDPTKAAHRQALLKVFSQALGIAGGVGGQKFDPQALREMEGVLLTGAVLESLTCGEGGNSEIGKILLVPEKQQAVLQAYRTTVCGLGAGQRAELFRQLSEELRDQIDTQRVFDQLRLEQSNMSGLLDQSRLGALLKNLADVERLLRDVDLLSGLARLLPKGACAGHQPPPQANTTSWSSNSTTWGPNTTDSSVEEGEGASESEAEAENPKSQFSAFVQLWAGLQPILCGNNRIFEPEALKQGNMSSLGFTSKEQRNLGLLVHLMTTNPKILYSPIDSEVDKVIQKANETFAFVGNVTHYARVWLNISAELRAFLEEGKLQNHLMWLQQFITDLKRHHELLNVSDSELMNSLLEENFTLPNTTTLLEQLHTIDNAACGWTNFMSKVSVDIFRGFPDEESIVNYTLNQAYHDNVSVFASVIFQTNKDGSLPPHVMYKIRQNSSFTEKTNEIRRAYWRPGPNTGGKFYFLYGFVWIQDMIERAIINTFVGHDVVEPGNYVQMFPYPCYTRDDFLFVIEHMMPLCMVISWVYSVAMMIQHIVAEKEQRLKEVMKMMGLNNAVHWVAWFITGFVQLSISVTALTAILKYGKVLLHSDPFIIWLFLTIYAVATIMFCFLVSVLYSKAKLASACGGIIYFLSYVPYMYVAIREEVAHDKITAFEKCIASLMSTTAFGLGSKYFALYEVAGVGIQWRTINQSPVEGDDFNLLLSMVMLTIDAIVYGVLTWYIEAVHPGMYGLPRPWYFPLQKSYWLGSGRIETWEWPWGGGTRLSVMEEDQACAMEHRRSEETRGIEEEPNHLPLVVCIDKLTKVYKTGSKLALNKLSLNLHENQVVSFLGHNGAGKTTTMSILTGLFPPTSGSATIYGHDIRTEMERIRQNLGMCPQHNVLFDKLSVEEHLWFYSRLKGMAEEDIRKEMDKMIEDLELSNKRHSLVQTLSGGMKRKLSVAIAFVGGSRAVILDEPTAGVDPYARRAIWDLILKYKQGRTILLSTHHMDEADLLGDRIAIISHGKLKCCGSPLFLKSTYGDGYKLTLVKKQSDSHTADQSSQSPSSSISPCSENRVTQFIRQYVASCLLVSDSNTELSYVLPSEAVKKGCFERLFQALEQSLDSLALTSFGVMDTTLEEVFLKVSEEDLSLENSDADMKDSPGGASAGKPSGLLGGPQCEGAPAAAVMRPEVELSNLMMCSRLSPSQASLQSGSSLGSVRGDEGGPYSEFYGDYCPLFDNGQDLDSASLRDEESSPQPTVLEGQGSFKLDGWWLKLRQFHGLIVKRFHCAKRNTKGIFSQILLPAFFVCVAMTVALSVPEIGDLPPLILSPSQYHNYTQPRGNFIPYANEDRSQYRTKFSPDAGPQRIVNTLRLPSGVGATCILKTPFNSTLDQLAQTLNPYANNSKTLAARYFDSMCLDSFTQGVPLSNFVPPPPSPAPSDDPEAHFEDGLWNYTAAPTTVREMATSPPTLPLSIHEPVRCICSMQGTGFSCPSGVGGRPPLMKVVTGDILVDITGRNVSEYLLYTSDRLRLHRYGGFTVGNIQKSVPASFGRKTPPMVRKIAVRRSSQVLYNNKGYHSMPTYLNVLNNAILRANLPSSKGNPSAYGITVTNHPMNRTSASLSLDYLLQGTDVVIAIFIIVAMSFVPASFVVFLVAEKSTKAKHLQFVSGCDPVTYWLANYIWDMLNYLVPATCCVLILFVFDLPAYTSPTNFPAVLSLFLLYGWSITPIMYPASFWFEVPSTAYVFLIVINLFIGITATVATFLLQLFERDKDLKLVNSYLKSCFLIFPNYNLGHGLMEMAYNEYINEYYAKIGQFDKVKSPFEWDIVTRGLVAMTIEGFVGFLITILCQYNFLRKAQRVPVNSQPVEDDDVDVACERRRVLRGDADNDMLKIDNLTKVYKSRKMGRILAVDRLCLGVRPGECFGLLGVNGAGKTTTFKMLTGDESTTGGEAFIQEHSILRELLRVQQSIGYCPQFDALFDDLTAREHLELYTRLRGIPWKDEERVVQWALEKLELSKYADKPAGTYSGGNKRKLSTAIALIGYPSLIFLDEPTTGMDPKARRFLWNLILDIIKTGRSVVLTSHSMEECEALCTRLGIMVNGRFKCLGSIQHLKNRFGDGYMITVRTKTTASVKEVIRFFNRNFPEAILKERHHTKIQYQLKSENISLAQVFSKMEQVVEVLSIEDYSVSQTTLDNVFVNFAKKQSDNLEQQESSPSSGGQSPLQHLLSLLRPRPANTELNALVSEEPEELESDDDEGLISFEEERVQLSFNTDTLC

Target ORF information:

RefSeq Version	XM_005165230.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165230.3

ORF Insert Sequence:

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG CCTTCTATAG CGCGGCGCCG 
CTCACCTCGG CGGGCATCAT TCCCATCATG CAGTCGTTGT GTCCGGATGG TCAGCGGGAT 
GAGTTTGGCT TCCTGCAGTA TAAAAACTCC ACTGTGACTC AATTGCTGGA AAGGATAAGT 
GAAGTTGTGG AGCAGAATCA CTTGTTTACA TCAGACAGGC CCAGTCTGGG AGAGGAGCTG 
GAGTCTCTGC AGCAGCACCT CGAAAGTCTG AGCTCAGCAC AAGGCCCTCT AGAGAGCCAC 
TACAACACCA GCCACGGTTT CACTGTGGGA AGCGTCCTCA GGAACCAGTC GATGTTCCAG 
CAGTTTCTGA TGCGAAATCT CTCCCTGCCG AACGACACGT CCAGCCTGCT GCTCTCCTCC 
CCCGTCAATC TAAAAGAGGT ATATAATCTC ATCTTTGGGA CGTATCCCAA AGCGGGATCA 
AAGGGAGGAG AAAGAGATGA AGGACAGCAC CAACTCAACA GTCCTGGAGA GAAGCTTGTA 
CAGCTGGAGA AGCAACTACT TGGAGGCTGG CACAGTGTAG ACGGGGGTCT GATTCAGAAG 
GCACTGAGAG ATCCCACAAA AGCTGCTCAC CGTCAAGCTC TTCTCAAAGT CTTCTCTCAG 
GCGTTGGGCA TCGCTGGCGG TGTAGGAGGT CAAAAATTCG ATCCTCAAGC ACTGCGTGAG 
ATGGAGGGAG TTCTGTTGAC TGGTGCAGTG CTTGAAAGCC TTACCTGTGG GGAAGGAGGG 
AACAGTGAGA TTGGCAAAAT CCTATTGGTC CCTGAGAAAC AACAGGCGGT TTTACAGGCT 
TACCGCACGA CTGTTTGTGG TTTAGGGGCG GGGCAGAGGG CGGAGCTTTT CAGACAGTTG 
AGTGAAGAAC TGAGAGATCA GATTGACACA CAGAGAGTCT TTGATCAGTT GCGTCTTGAG 
CAGTCTAATA TGTCTGGTCT GCTGGATCAG TCCCGTCTCG GGGCTCTCCT GAAGAACCTG 
GCTGATGTGG AGCGGCTGCT GAGGGACGTG GATCTACTGT CAGGTCTGGC CAGACTGCTG 
CCGAAAGGGG CCTGTGCGGG TCACCAGCCT CCTCCTCAGG CCAACACCAC CTCCTGGAGC 
TCCAACTCTA CCACATGGGG TCCCAATACA ACTGACAGTT CTGTGGAGGA AGGAGAAGGA 
GCTTCTGAGA GCGAGGCAGA GGCTGAAAAC CCCAAATCAC AGTTTTCTGC ATTTGTTCAG 
CTGTGGGCAG GACTTCAACC CATACTTTGT GGGAATAATA GGATTTTCGA ACCAGAAGCG 
CTGAAACAAG GAAATATGAG TTCTCTGGGC TTCACCAGCA AAGAACAGAG AAATTTAGGA 
TTACTTGTTC ATCTCATGAC TACAAATCCC AAAATCCTCT ACTCTCCCAT TGACTCAGAG 
GTGGACAAGG TCATACAAAA GGCCAATGAG ACCTTTGCTT TTGTGGGGAA TGTGACTCAC 
TATGCCCGTG TGTGGCTCAA CATCTCTGCT GAATTAAGGG CCTTTTTAGA GGAGGGTAAA 
TTGCAAAATC ACCTGATGTG GCTCCAGCAG TTTATTACCG ACCTGAAGAG GCACCATGAG 
CTGCTGAACG TATCAGACAG TGAGCTGATG AACAGTCTGC TGGAGGAGAA CTTCACCCTG 
CCAAACACAA CCACCTTACT GGAGCAGCTG CACACCATTG ATAATGCTGC TTGTGGATGG 
ACTAACTTCA TGTCAAAGGT CAGTGTGGAT ATCTTCAGAG GCTTCCCTGA TGAAGAAAGC 
ATTGTCAATT ACACACTGAA CCAGGCCTAC CACGACAACG TCTCAGTCTT TGCAAGTGTG 
ATTTTCCAGA CCAATAAAGA TGGATCCCTG CCACCCCATG TTATGTATAA AATTCGACAG 
AACTCGAGCT TTACCGAGAA AACCAATGAA ATCCGACGTG CTTATTGGCG CCCAGGGCCA 
AACACAGGCG GCAAATTCTA CTTCCTTTAT GGCTTTGTGT GGATCCAAGA TATGATTGAA 
CGGGCAATCA TCAACACCTT TGTTGGCCAC GATGTTGTGG AGCCTGGAAA TTACGTGCAG 
ATGTTTCCTT ATCCGTGCTA CACCAGAGAC GATTTTCTCT TTGTTATCGA GCACATGATG 
CCTCTGTGTA TGGTCATATC CTGGGTCTAC TCTGTGGCCA TGATGATCCA GCACATCGTA 
GCAGAGAAGG AGCAAAGACT GAAAGAGGTA ATGAAGATGA TGGGCTTGAA TAACGCAGTG 
CACTGGGTGG CCTGGTTCAT CACAGGCTTC GTGCAGCTTT CCATCTCAGT GACGGCCCTC 
ACTGCCATAC TGAAATACGG GAAAGTCCTC CTTCACAGCG ATCCCTTCAT CATATGGCTT 
TTTCTTACAA TCTACGCTGT CGCAACCATC ATGTTCTGTT TCCTGGTGTC GGTGTTGTAC 
TCTAAAGCCA AACTGGCCTC GGCATGTGGA GGAATCATCT ACTTCCTGAG TTATGTGCCC 
TACATGTATG TGGCCATAAG AGAAGAGGTG GCTCACGATA AGATCACTGC CTTTGAGAAG 
TGCATAGCGT CTTTGATGTC TACCACAGCG TTCGGTCTGG GATCCAAGTA CTTTGCCCTG 
TATGAAGTGG CTGGCGTGGG AATTCAGTGG CGCACTATTA ACCAGTCACC TGTAGAGGGC 
GATGACTTCA ATCTACTGCT GTCTATGGTC ATGCTCACTA TTGACGCCAT TGTATATGGT 
GTGTTGACCT GGTATATAGA AGCCGTCCAT CCAGGGATGT ATGGACTGCC TCGGCCATGG 
TATTTCCCCT TGCAGAAGTC CTATTGGTTA GGTAGTGGCC GCATAGAGAC GTGGGAGTGG 
CCGTGGGGTG GAGGCACCAG GCTGAGTGTT ATGGAAGAGG ACCAGGCTTG TGCCATGGAG 
CATAGACGCT CAGAGGAAAC CCGTGGAATT GAAGAAGAGC CCAATCATTT GCCGCTGGTG 
GTGTGCATCG ACAAACTGAC CAAAGTCTAC AAGACGGGCA GCAAACTGGC TCTAAACAAG 
CTGAGCCTGA ACCTGCATGA GAACCAGGTT GTCTCTTTCT TGGGACACAA TGGGGCTGGC 
AAGACCACCA CTATGTCCAT TCTCACGGGA CTCTTTCCAC CCACCTCTGG CTCTGCCACC 
ATATACGGCC ATGACATCCG CACAGAGATG GAGCGCATCA GGCAGAACTT GGGCATGTGT 
CCCCAGCACA ACGTCCTGTT TGATAAGCTC AGTGTGGAGG AACATCTGTG GTTCTACTCA 
CGGCTGAAAG GCATGGCAGA GGAAGATATT CGCAAGGAGA TGGACAAGAT GATAGAGGAT 
CTCGAATTAT CTAATAAACG TCACAGTCTG GTTCAAACCC TGTCAGGAGG GATGAAAAGG 
AAGCTGTCAG TGGCCATTGC TTTTGTAGGT GGGTCTCGGG CAGTGATTTT GGATGAACCA 
ACAGCAGGAG TAGACCCATA TGCCCGTCGA GCGATCTGGG ACCTCATCCT GAAATATAAG 
CAAGGTCGCA CCATCCTGCT GTCCACTCAC CACATGGATG AGGCTGATCT GCTGGGTGAC 
CGCATTGCCA TCATCTCTCA TGGCAAGCTC AAGTGCTGCG GTTCTCCTCT GTTCCTCAAG 
AGCACATATG GAGACGGATA CAAACTCACA CTTGTTAAAA AACAGAGTGA CTCACACACT 
GCAGATCAGT CAAGCCAGTC TCCGTCCTCT TCGATATCTC CATGTTCAGA AAACAGAGTA 
ACTCAGTTCA TCCGTCAGTA TGTTGCTTCC TGCCTGCTGG TGTCCGACTC CAACACTGAA 
CTCTCTTATG TCCTACCATC AGAGGCTGTC AAGAAAGGCT GCTTTGAGAG ACTCTTCCAG 
GCTCTGGAGC AGAGTTTAGA CAGCTTGGCC TTGACCAGCT TTGGCGTGAT GGACACCACA 
TTGGAGGAGG TGTTTCTAAA GGTGTCAGAA GAAGATCTGT CGCTGGAAAA CAGCGATGCA 
GACATGAAGG ACTCTCCAGG CGGAGCATCA GCCGGGAAGC CGTCAGGTCT GTTAGGAGGG 
CCTCAGTGTG AAGGAGCTCC AGCCGCTGCT GTGATGAGGC CAGAGGTGGA GCTCAGCAAC 
CTGATGATGT GCTCCAGGCT GAGCCCAAGC CAAGCATCCC TGCAGTCCGG CTCTTCTCTG 
GGCTCAGTGA GAGGAGATGA AGGGGGTCCC TATTCTGAGT TCTATGGAGA CTATTGCCCA 
CTGTTTGACA ACGGTCAGGA TCTGGACTCT GCCAGTCTCA GAGATGAAGA GTCATCTCCT 
CAGCCCACTG TACTCGAGGG CCAGGGCAGC TTCAAGCTCG ATGGCTGGTG GCTTAAACTC 
CGCCAGTTTC ATGGGCTGAT CGTGAAAAGG TTTCACTGTG CCAAGAGGAA CACCAAGGGC 
ATTTTCTCTC AGATTCTGCT GCCTGCTTTC TTCGTATGTG TGGCAATGAC TGTGGCTCTG 
TCTGTGCCTG AGATTGGTGA CCTTCCTCCC CTGATCCTGT CTCCATCTCA ATATCACAAC 
TACACACAGC CTCGAGGAAA CTTCATCCCC TACGCCAATG AAGACAGATC TCAATACAGG 
ACTAAGTTTT CTCCAGACGC AGGCCCACAG AGGATTGTTA ACACCCTCCG TCTGCCATCT 
GGAGTCGGTG CGACTTGTAT CCTGAAGACT CCCTTTAACA GCACCCTGGA CCAACTGGCC 
CAAACTCTCA ATCCTTATGC CAACAACTCT AAAACCCTGG CAGCCCGCTA CTTTGACTCT 
ATGTGCCTGG ACTCCTTCAC GCAGGGTGTG CCTTTGTCGA ATTTCGTTCC CCCTCCGCCC 
TCTCCTGCCC CCTCAGACGA CCCGGAGGCT CATTTTGAAG ACGGCCTGTG GAACTACACT 
GCAGCTCCTA CTACTGTCAG AGAGATGGCC ACGTCTCCCC CGACTCTGCC CTTGAGTATC 
CATGAGCCGG TGCGCTGTAT CTGCTCTATG CAGGGCACAG GCTTCTCCTG CCCCAGTGGA 
GTTGGGGGTC GACCTCCTCT GATGAAAGTG GTGACCGGAG ACATCCTGGT GGACATTACA 
GGTCGCAACG TGTCTGAATA TCTCCTGTAC ACGTCTGATC GGCTCAGGTT ACACAGATAT 
GGAGGCTTTA CTGTTGGAAA CATTCAGAAA TCTGTCCCAG CATCCTTCGG GAGAAAAACA 
CCACCTATGG TGCGCAAAAT AGCAGTGAGG CGATCCTCAC AGGTGCTGTA TAATAACAAA 
GGCTATCACA GTATGCCAAC ATATCTAAAT GTCCTCAACA ATGCCATTCT CAGAGCCAAT 
CTGCCCAGTA GTAAAGGAAA CCCTTCTGCA TACGGCATTA CAGTGACCAA TCATCCCATG 
AACAGAACCA GTGCCAGTCT TTCTCTGGAT TATCTGCTCC AGGGCACTGA TGTGGTTATT 
GCCATCTTCA TCATTGTAGC CATGTCTTTC GTGCCTGCGA GCTTTGTGGT CTTTCTGGTA 
GCGGAAAAAT CCACCAAAGC CAAACACCTG CAGTTTGTCA GCGGATGCGA CCCTGTCACG 
TACTGGCTAG CAAACTACAT ATGGGACATG CTGAACTACC TGGTTCCAGC TACATGCTGT 
GTCCTAATTT TGTTTGTGTT CGACCTTCCT GCGTACACGT CACCTACAAA TTTCCCAGCA 
GTGCTCTCTC TGTTTCTGCT TTATGGCTGG TCTATAACCC CCATCATGTA CCCAGCCTCC 
TTCTGGTTTG AGGTGCCCAG CACAGCTTAT GTTTTCCTCA TAGTCATCAA TCTCTTCATT 
GGCATCACAG CCACGGTGGC CACTTTCCTC CTCCAGCTGT TTGAGCGCGA CAAGGACCTG 
AAGTTGGTAA ACAGCTACCT GAAGTCATGT TTTCTCATCT TCCCCAACTA TAACCTGGGT 
CACGGATTGA TGGAGATGGC CTATAATGAG TATATCAATG AATATTACGC CAAGATAGGC 
CAGTTTGATA AAGTGAAGTC TCCATTTGAA TGGGACATTG TGACGCGGGG ACTGGTAGCA 
ATGACCATTG AGGGCTTCGT CGGCTTTCTC ATTACAATTC TGTGCCAGTA CAACTTCCTC 
AGGAAAGCCC AGAGAGTGCC TGTCAACAGT CAGCCAGTGG AGGACGATGA TGTTGATGTG 
GCCTGTGAAA GACGCCGAGT TCTGAGGGGA GACGCTGACA ACGACATGCT GAAGATCGAC 
AACCTAACCA AGGTGTACAA GTCTCGTAAG ATGGGCCGGA TCCTGGCAGT GGACCGATTG 
TGTCTGGGGG TCCGTCCTGG TGAATGCTTC GGTCTGCTGG GAGTGAATGG GGCAGGAAAG 
ACCACCACCT TCAAAATGTT GACAGGAGAT GAGAGCACAA CAGGGGGAGA AGCCTTTATC 
CAGGAACACA GTATCTTGCG GGAGCTGTTA CGTGTGCAGC AGAGCATCGG TTACTGCCCT 
CAGTTTGACG CTCTGTTTGA TGATCTGACG GCTCGAGAGC ATCTGGAGCT CTACACGCGC 
CTCCGCGGCA TCCCATGGAA AGATGAGGAG CGGGTGGTTC AGTGGGCTTT GGAGAAGTTG 
GAGTTGTCCA AGTATGCCGA CAAGCCTGCT GGCACCTACA GCGGAGGCAA CAAACGCAAA 
CTCTCTACAG CCATTGCTCT GATTGGATAC CCATCGCTCA TCTTTTTGGA TGAGCCCACC 
ACAGGTATGG ACCCAAAGGC TCGCCGTTTC TTATGGAACC TAATTCTTGA CATCATCAAG 
ACGGGCCGCT CAGTAGTGTT GACGTCACAC AGTATGGAGG AGTGTGAAGC GCTGTGCACT 
AGACTGGGCA TCATGGTTAA TGGCAGGTTC AAGTGTCTAG GAAGCATCCA GCATCTCAAA 
AACAGATTCG GAGATGGATA CATGATAACA GTACGCACTA AGACCACCGC TAGCGTTAAA 
GAAGTGATTC GGTTTTTTAA CAGAAATTTT CCTGAGGCAA TTCTAAAGGA GCGACACCAC 
ACAAAGATTC AGTACCAGCT GAAGTCAGAG AACATCTCTC TGGCTCAGGT CTTCAGCAAA 
ATGGAGCAGG TGGTGGAGGT GCTGAGCATC GAGGATTATT CCGTCAGCCA AACCACACTG 
GATAACGTCT TTGTTAACTT TGCAAAAAAG CAAAGCGACA ACTTAGAGCA GCAAGAAAGT 
TCTCCATCTA GTGGTGGCCA GTCGCCACTG CAGCATCTGC TCAGCCTCCT CAGGCCTCGT 
CCTGCAAACA CTGAGCTCAA CGCCCTCGTG AGTGAAGAGC CTGAGGAACT GGAGAGTGAT 
GATGACGAAG GACTGATCAG CTTTGAGGAA GAGAGGGTGC AGTTGTCCTT TAATACAGAC 
ACTCTGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

abca2 ( XM_005165231.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165288.1 Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X3, mRNA.

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CloneID	ODa10383
Clone ID Related Accession (Same CDS sequence)	XM_005165231.3 , XM_005165231.4
Accession Version	XM_005165231.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ATP-binding cassette sub-family A member 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165231.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG TGTCCTTCTA TAGCGCGGCG 
CCGCTCACCT CGGCGGGCAT CATTCCCATC ATGCAGTCGT TGTGTCCGGA TGGTCAGCGG 
GATGAGTTTG GCTTCCTGCA GTATAAAAAC TCCACTGTGA CTCAATTGCT GGAAAGGATA 
AGTGAAGTTG TGGAGCAGAA TCACTTGTTT ACATCAGACA GGCCCAGTCT GGGAGAGGAG 
CTGGAGTCTC TGCAGCAGCA CCTCGAAAGT CTGAGCTCAG CACAAGGCCC TCTAGAGAGC 
CACTACAACA CCAGCCACGG TTTCACTGTG GGAAGCGTCC TCAGGAACCA GTCGATGTTC 
CAGCAGTTTC TGATGCGAAA TCTCTCCCTG CCGAACGACA CGTCCAGCCT GCTGCTCTCC 
TCCCCCGTCA ATCTAAAAGA GGTATATAAT CTCATCTTTG GGACGTATCC CAAAGCGGGA 
TCAAAGGGAG GAGAAAGAGA TGAAGGACAG CACCAACTCA ACAGTCCTGG AGAGAAGCTT 
GTACAGCTGG AGCAACTACT TGGAGGCTGG CACAGTGTAG ACGGGGGTCT GATTCAGAAG 
GCACTGAGAG ATCCCACAAA AGCTGCTCAC CGTCAAGCTC TTCTCAAAGT CTTCTCTCAG 
GCGTTGGGCA TCGCTGGCGG TGTAGGAGGT CAAAAATTCG ATCCTCAAGC ACTGCGTGAG 
ATGGAGGGAG TTCTGTTGAC TGGTGCAGTG CTTGAAAGCC TTACCTGTGG GGAAGGAGGG 
AACAGTGAGA TTGGCAAAAT CCTATTGGTC CCTGAGAAAC AACAGGCGGT TTTACAGGCT 
TACCGCACGA CTGTTTGTGG TTTAGGGGCG GGGCAGAGGG CGGAGCTTTT CAGACAGTTG 
AGTGAAGAAC TGAGAGATCA GATTGACACA CAGAGAGTCT TTGATCAGTT GCGTCTTGAG 
CAGTCTAATA TGTCTGGTCT GCTGGATCAG TCCCGTCTCG GGGCTCTCCT GAAGAACCTG 
GCTGATGTGG AGCGGCTGCT GAGGGACGTG GATCTACTGT CAGGTCTGGC CAGACTGCTG 
CCGAAAGGGG CCTGTGCGGG TCACCAGCCT CCTCCTCAGG CCAACACCAC CTCCTGGAGC 
TCCAACTCTA CCACATGGGG TCCCAATACA ACTGACAGTT CTGTGGAGGA AGGAGAAGGA 
GCTTCTGAGA GCGAGGCAGA GGCTGAAAAC CCCAAATCAC AGTTTTCTGC ATTTGTTCAG 
CTGTGGGCAG GACTTCAACC CATACTTTGT GGGAATAATA GGATTTTCGA ACCAGAAGCG 
CTGAAACAAG GAAATATGAG TTCTCTGGGC TTCACCAGCA AAGAACAGAG AAATTTAGGA 
TTACTTGTTC ATCTCATGAC TACAAATCCC AAAATCCTCT ACTCTCCCAT TGACTCAGAG 
GTGGACAAGG TCATACAAAA GGCCAATGAG ACCTTTGCTT TTGTGGGGAA TGTGACTCAC 
TATGCCCGTG TGTGGCTCAA CATCTCTGCT GAATTAAGGG CCTTTTTAGA GGAGGGTAAA 
TTGCAAAATC ACCTGATGTG GCTCCAGCAG TTTATTACCG ACCTGAAGAG GCACCATGAG 
CTGCTGAACG TATCAGACAG TGAGCTGATG AACAGTCTGC TGGAGGAGAA CTTCACCCTG 
CCAAACACAA CCACCTTACT GGAGCAGCTG CACACCATTG ATAATGCTGC TTGTGGATGG 
ACTAACTTCA TGTCAAAGGT CAGTGTGGAT ATCTTCAGAG GCTTCCCTGA TGAAGAAAGC 
ATTGTCAATT ACACACTGAA CCAGGCCTAC CACGACAACG TCTCAGTCTT TGCAAGTGTG 
ATTTTCCAGA CCAATAAAGA TGGATCCCTG CCACCCCATG TTATGTATAA AATTCGACAG 
AACTCGAGCT TTACCGAGAA AACCAATGAA ATCCGACGTG CTTATTGGCG CCCAGGGCCA 
AACACAGGCG GCAAATTCTA CTTCCTTTAT GGCTTTGTGT GGATCCAAGA TATGATTGAA 
CGGGCAATCA TCAACACCTT TGTTGGCCAC GATGTTGTGG AGCCTGGAAA TTACGTGCAG 
ATGTTTCCTT ATCCGTGCTA CACCAGAGAC GATTTTCTCT TTGTTATCGA GCACATGATG 
CCTCTGTGTA TGGTCATATC CTGGGTCTAC TCTGTGGCCA TGATGATCCA GCACATCGTA 
GCAGAGAAGG AGCAAAGACT GAAAGAGGTA ATGAAGATGA TGGGCTTGAA TAACGCAGTG 
CACTGGGTGG CCTGGTTCAT CACAGGCTTC GTGCAGCTTT CCATCTCAGT GACGGCCCTC 
ACTGCCATAC TGAAATACGG GAAAGTCCTC CTTCACAGCG ATCCCTTCAT CATATGGCTT 
TTTCTTACAA TCTACGCTGT CGCAACCATC ATGTTCTGTT TCCTGGTGTC GGTGTTGTAC 
TCTAAAGCCA AACTGGCCTC GGCATGTGGA GGAATCATCT ACTTCCTGAG TTATGTGCCC 
TACATGTATG TGGCCATAAG AGAAGAGGTG GCTCACGATA AGATCACTGC CTTTGAGAAG 
TGCATAGCGT CTTTGATGTC TACCACAGCG TTCGGTCTGG GATCCAAGTA CTTTGCCCTG 
TATGAAGTGG CTGGCGTGGG AATTCAGTGG CGCACTATTA ACCAGTCACC TGTAGAGGGC 
GATGACTTCA ATCTACTGCT GTCTATGGTC ATGCTCACTA TTGACGCCAT TGTATATGGT 
GTGTTGACCT GGTATATAGA AGCCGTCCAT CCAGGGATGT ATGGACTGCC TCGGCCATGG 
TATTTCCCCT TGCAGAAGTC CTATTGGTTA GGTAGTGGCC GCATAGAGAC GTGGGAGTGG 
CCGTGGGGTG GAGGCACCAG GCTGAGTGTT ATGGAAGAGG ACCAGGCTTG TGCCATGGAG 
CATAGACGCT CAGAGGAAAC CCGTGGAATT GAAGAAGAGC CCAATCATTT GCCGCTGGTG 
GTGTGCATCG ACAAACTGAC CAAAGTCTAC AAGACGGGCA GCAAACTGGC TCTAAACAAG 
CTGAGCCTGA ACCTGCATGA GAACCAGGTT GTCTCTTTCT TGGGACACAA TGGGGCTGGC 
AAGACCACCA CTATGTCCAT TCTCACGGGA CTCTTTCCAC CCACCTCTGG CTCTGCCACC 
ATATACGGCC ATGACATCCG CACAGAGATG GAGCGCATCA GGCAGAACTT GGGCATGTGT 
CCCCAGCACA ACGTCCTGTT TGATAAGCTC AGTGTGGAGG AACATCTGTG GTTCTACTCA 
CGGCTGAAAG GCATGGCAGA GGAAGATATT CGCAAGGAGA TGGACAAGAT GATAGAGGAT 
CTCGAATTAT CTAATAAACG TCACAGTCTG GTTCAAACCC TGTCAGGAGG GATGAAAAGG 
AAGCTGTCAG TGGCCATTGC TTTTGTAGGT GGGTCTCGGG CAGTGATTTT GGATGAACCA 
ACAGCAGGAG TAGACCCATA TGCCCGTCGA GCGATCTGGG ACCTCATCCT GAAATATAAG 
CAAGGTCGCA CCATCCTGCT GTCCACTCAC CACATGGATG AGGCTGATCT GCTGGGTGAC 
CGCATTGCCA TCATCTCTCA TGGCAAGCTC AAGTGCTGCG GTTCTCCTCT GTTCCTCAAG 
AGCACATATG GAGACGGATA CAAACTCACA CTTGTTAAAA AACAGAGTGA CTCACACACT 
GCAGATCAGT CAAGCCAGTC TCCGTCCTCT TCGATATCTC CATGTTCAGA AAACAGAGTA 
ACTCAGTTCA TCCGTCAGTA TGTTGCTTCC TGCCTGCTGG TGTCCGACTC CAACACTGAA 
CTCTCTTATG TCCTACCATC AGAGGCTGTC AAGAAAGGCT GCTTTGAGAG ACTCTTCCAG 
GCTCTGGAGC AGAGTTTAGA CAGCTTGGCC TTGACCAGCT TTGGCGTGAT GGACACCACA 
TTGGAGGAGG TGTTTCTAAA GGTGTCAGAA GAAGATCTGT CGCTGGAAAA CAGCGATGCA 
GACATGAAGG ACTCTCCAGG CGGAGCATCA GCCGGGAAGC CGTCAGGTCT GTTAGGAGGG 
CCTCAGTGTG AAGGAGCTCC AGCCGCTGCT GTGATGAGGC CAGAGGTGGA GCTCAGCAAC 
CTGATGATGT GCTCCAGGCT GAGCCCAAGC CAAGCATCCC TGCAGTCCGG CTCTTCTCTG 
GGCTCAGTGA GAGGAGATGA AGGGGGTCCC TATTCTGAGT TCTATGGAGA CTATTGCCCA 
CTGTTTGACA ACGGTCAGGA TCTGGACTCT GCCAGTCTCA GAGATGAAGA GTCATCTCCT 
CAGCCCACTG TACTCGAGGG CCAGGGCAGC TTCAAGCTCG ATGGCTGGTG GCTTAAACTC 
CGCCAGTTTC ATGGGCTGAT CGTGAAAAGG TTTCACTGTG CCAAGAGGAA CACCAAGGGC 
ATTTTCTCTC AGATTCTGCT GCCTGCTTTC TTCGTATGTG TGGCAATGAC TGTGGCTCTG 
TCTGTGCCTG AGATTGGTGA CCTTCCTCCC CTGATCCTGT CTCCATCTCA ATATCACAAC 
TACACACAGC CTCGAGGAAA CTTCATCCCC TACGCCAATG AAGACAGATC TCAATACAGG 
ACTAAGTTTT CTCCAGACGC AGGCCCACAG AGGATTGTTA ACACCCTCCG TCTGCCATCT 
GGAGTCGGTG CGACTTGTAT CCTGAAGACT CCCTTTAACA GCACCCTGGA CCAACTGGCC 
CAAACTCTCA ATCCTTATGC CAACAACTCT AAAACCCTGG CAGCCCGCTA CTTTGACTCT 
ATGTGCCTGG ACTCCTTCAC GCAGGGTGTG CCTTTGTCGA ATTTCGTTCC CCCTCCGCCC 
TCTCCTGCCC CCTCAGACGA CCCGGAGGCT CATTTTGAAG ACGGCCTGTG GAACTACACT 
GCAGCTCCTA CTACTGTCAG AGAGATGGCC ACGTCTCCCC CGACTCTGCC CTTGAGTATC 
CATGAGCCGG TGCGCTGTAT CTGCTCTATG CAGGGCACAG GCTTCTCCTG CCCCAGTGGA 
GTTGGGGGTC GACCTCCTCT GATGAAAGTG GTGACCGGAG ACATCCTGGT GGACATTACA 
GGTCGCAACG TGTCTGAATA TCTCCTGTAC ACGTCTGATC GGCTCAGGTT ACACAGATAT 
GGAGGCTTTA CTGTTGGAAA CATTCAGAAA TCTGTCCCAG CATCCTTCGG GAGAAAAACA 
CCACCTATGG TGCGCAAAAT AGCAGTGAGG CGATCCTCAC AGGTGCTGTA TAATAACAAA 
GGCTATCACA GTATGCCAAC ATATCTAAAT GTCCTCAACA ATGCCATTCT CAGAGCCAAT 
CTGCCCAGTA GTAAAGGAAA CCCTTCTGCA TACGGCATTA CAGTGACCAA TCATCCCATG 
AACAGAACCA GTGCCAGTCT TTCTCTGGAT TATCTGCTCC AGGGCACTGA TGTGGTTATT 
GCCATCTTCA TCATTGTAGC CATGTCTTTC GTGCCTGCGA GCTTTGTGGT CTTTCTGGTA 
GCGGAAAAAT CCACCAAAGC CAAACACCTG CAGTTTGTCA GCGGATGCGA CCCTGTCACG 
TACTGGCTAG CAAACTACAT ATGGGACATG CTGAACTACC TGGTTCCAGC TACATGCTGT 
GTCCTAATTT TGTTTGTGTT CGACCTTCCT GCGTACACGT CACCTACAAA TTTCCCAGCA 
GTGCTCTCTC TGTTTCTGCT TTATGGCTGG TCTATAACCC CCATCATGTA CCCAGCCTCC 
TTCTGGTTTG AGGTGCCCAG CACAGCTTAT GTTTTCCTCA TAGTCATCAA TCTCTTCATT 
GGCATCACAG CCACGGTGGC CACTTTCCTC CTCCAGCTGT TTGAGCGCGA CAAGGACCTG 
AAGTTGGTAA ACAGCTACCT GAAGTCATGT TTTCTCATCT TCCCCAACTA TAACCTGGGT 
CACGGATTGA TGGAGATGGC CTATAATGAG TATATCAATG AATATTACGC CAAGATAGGC 
CAGTTTGATA AAGTGAAGTC TCCATTTGAA TGGGACATTG TGACGCGGGG ACTGGTAGCA 
ATGACCATTG AGGGCTTCGT CGGCTTTCTC ATTACAATTC TGTGCCAGTA CAACTTCCTC 
AGGAAAGCCC AGAGAGTGCC TGTCAACAGT CAGCCAGTGG AGGACGATGA TGTTGATGTG 
GCCTGTGAAA GACGCCGAGT TCTGAGGGGA GACGCTGACA ACGACATGCT GAAGATCGAC 
AACCTAACCA AGGTGTACAA GTCTCGTAAG ATGGGCCGGA TCCTGGCAGT GGACCGATTG 
TGTCTGGGGG TCCGTCCTGG TGAATGCTTC GGTCTGCTGG GAGTGAATGG GGCAGGAAAG 
ACCACCACCT TCAAAATGTT GACAGGAGAT GAGAGCACAA CAGGGGGAGA AGCCTTTATC 
CAGGAACACA GTATCTTGCG GGAGCTGTTA CGTGTGCAGC AGAGCATCGG TTACTGCCCT 
CAGTTTGACG CTCTGTTTGA TGATCTGACG GCTCGAGAGC ATCTGGAGCT CTACACGCGC 
CTCCGCGGCA TCCCATGGAA AGATGAGGAG CGGGTGGTTC AGTGGGCTTT GGAGAAGTTG 
GAGTTGTCCA AGTATGCCGA CAAGCCTGCT GGCACCTACA GCGGAGGCAA CAAACGCAAA 
CTCTCTACAG CCATTGCTCT GATTGGATAC CCATCGCTCA TCTTTTTGGA TGAGCCCACC 
ACAGGTATGG ACCCAAAGGC TCGCCGTTTC TTATGGAACC TAATTCTTGA CATCATCAAG 
ACGGGCCGCT CAGTAGTGTT GACGTCACAC AGTATGGAGG AGTGTGAAGC GCTGTGCACT 
AGACTGGGCA TCATGGTTAA TGGCAGGTTC AAGTGTCTAG GAAGCATCCA GCATCTCAAA 
AACAGATTCG GAGATGGATA CATGATAACA GTACGCACTA AGACCACCGC TAGCGTTAAA 
GAAGTGATTC GGTTTTTTAA CAGAAATTTT CCTGAGGCAA TTCTAAAGGA GCGACACCAC 
ACAAAGATTC AGTACCAGCT GAAGTCAGAG AACATCTCTC TGGCTCAGGT CTTCAGCAAA 
ATGGAGCAGG TGGTGGAGGT GCTGAGCATC GAGGATTATT CCGTCAGCCA AACCACACTG 
GATAACGTCT TTGTTAACTT TGCAAAAAAG CAAAGCGACA ACTTAGAGCA GCAAGAAAGT 
TCTCCATCTA GTGGTGGCCA GTCGCCACTG CAGCATCTGC TCAGCCTCCT CAGGCCTCGT 
CCTGCAAACA CTGAGCTCAA CGCCCTCGTG AGTGAAGAGC CTGAGGAACT GGAGAGTGAT 
GATGACGAAG GACTGATCAG CTTTGAGGAA GAGAGGGTGC AGTTGTCCTT TAATACAGAC 
ACTCTGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165288.1
CDS	552..8063
Translation	MGFLHQLHLLLWKNVTLKRRGPWVLAFEIFIPLVLFFILLGLRQKKPAIPVKEVSFYSAAPLTSAGIIPIMQSLCPDGQRDEFGFLQYKNSTVTQLLERISEVVEQNHLFTSDRPSLGEELESLQQHLESLSSAQGPLESHYNTSHGFTVGSVLRNQSMFQQFLMRNLSLPNDTSSLLLSSPVNLKEVYNLIFGTYPKAGSKGGERDEGQHQLNSPGEKLVQLEQLLGGWHSVDGGLIQKALRDPTKAAHRQALLKVFSQALGIAGGVGGQKFDPQALREMEGVLLTGAVLESLTCGEGGNSEIGKILLVPEKQQAVLQAYRTTVCGLGAGQRAELFRQLSEELRDQIDTQRVFDQLRLEQSNMSGLLDQSRLGALLKNLADVERLLRDVDLLSGLARLLPKGACAGHQPPPQANTTSWSSNSTTWGPNTTDSSVEEGEGASESEAEAENPKSQFSAFVQLWAGLQPILCGNNRIFEPEALKQGNMSSLGFTSKEQRNLGLLVHLMTTNPKILYSPIDSEVDKVIQKANETFAFVGNVTHYARVWLNISAELRAFLEEGKLQNHLMWLQQFITDLKRHHELLNVSDSELMNSLLEENFTLPNTTTLLEQLHTIDNAACGWTNFMSKVSVDIFRGFPDEESIVNYTLNQAYHDNVSVFASVIFQTNKDGSLPPHVMYKIRQNSSFTEKTNEIRRAYWRPGPNTGGKFYFLYGFVWIQDMIERAIINTFVGHDVVEPGNYVQMFPYPCYTRDDFLFVIEHMMPLCMVISWVYSVAMMIQHIVAEKEQRLKEVMKMMGLNNAVHWVAWFITGFVQLSISVTALTAILKYGKVLLHSDPFIIWLFLTIYAVATIMFCFLVSVLYSKAKLASACGGIIYFLSYVPYMYVAIREEVAHDKITAFEKCIASLMSTTAFGLGSKYFALYEVAGVGIQWRTINQSPVEGDDFNLLLSMVMLTIDAIVYGVLTWYIEAVHPGMYGLPRPWYFPLQKSYWLGSGRIETWEWPWGGGTRLSVMEEDQACAMEHRRSEETRGIEEEPNHLPLVVCIDKLTKVYKTGSKLALNKLSLNLHENQVVSFLGHNGAGKTTTMSILTGLFPPTSGSATIYGHDIRTEMERIRQNLGMCPQHNVLFDKLSVEEHLWFYSRLKGMAEEDIRKEMDKMIEDLELSNKRHSLVQTLSGGMKRKLSVAIAFVGGSRAVILDEPTAGVDPYARRAIWDLILKYKQGRTILLSTHHMDEADLLGDRIAIISHGKLKCCGSPLFLKSTYGDGYKLTLVKKQSDSHTADQSSQSPSSSISPCSENRVTQFIRQYVASCLLVSDSNTELSYVLPSEAVKKGCFERLFQALEQSLDSLALTSFGVMDTTLEEVFLKVSEEDLSLENSDADMKDSPGGASAGKPSGLLGGPQCEGAPAAAVMRPEVELSNLMMCSRLSPSQASLQSGSSLGSVRGDEGGPYSEFYGDYCPLFDNGQDLDSASLRDEESSPQPTVLEGQGSFKLDGWWLKLRQFHGLIVKRFHCAKRNTKGIFSQILLPAFFVCVAMTVALSVPEIGDLPPLILSPSQYHNYTQPRGNFIPYANEDRSQYRTKFSPDAGPQRIVNTLRLPSGVGATCILKTPFNSTLDQLAQTLNPYANNSKTLAARYFDSMCLDSFTQGVPLSNFVPPPPSPAPSDDPEAHFEDGLWNYTAAPTTVREMATSPPTLPLSIHEPVRCICSMQGTGFSCPSGVGGRPPLMKVVTGDILVDITGRNVSEYLLYTSDRLRLHRYGGFTVGNIQKSVPASFGRKTPPMVRKIAVRRSSQVLYNNKGYHSMPTYLNVLNNAILRANLPSSKGNPSAYGITVTNHPMNRTSASLSLDYLLQGTDVVIAIFIIVAMSFVPASFVVFLVAEKSTKAKHLQFVSGCDPVTYWLANYIWDMLNYLVPATCCVLILFVFDLPAYTSPTNFPAVLSLFLLYGWSITPIMYPASFWFEVPSTAYVFLIVINLFIGITATVATFLLQLFERDKDLKLVNSYLKSCFLIFPNYNLGHGLMEMAYNEYINEYYAKIGQFDKVKSPFEWDIVTRGLVAMTIEGFVGFLITILCQYNFLRKAQRVPVNSQPVEDDDVDVACERRRVLRGDADNDMLKIDNLTKVYKSRKMGRILAVDRLCLGVRPGECFGLLGVNGAGKTTTFKMLTGDESTTGGEAFIQEHSILRELLRVQQSIGYCPQFDALFDDLTAREHLELYTRLRGIPWKDEERVVQWALEKLELSKYADKPAGTYSGGNKRKLSTAIALIGYPSLIFLDEPTTGMDPKARRFLWNLILDIIKTGRSVVLTSHSMEECEALCTRLGIMVNGRFKCLGSIQHLKNRFGDGYMITVRTKTTASVKEVIRFFNRNFPEAILKERHHTKIQYQLKSENISLAQVFSKMEQVVEVLSIEDYSVSQTTLDNVFVNFAKKQSDNLEQQESSPSSGGQSPLQHLLSLLRPRPANTELNALVSEEPEELESDDDEGLISFEEERVQLSFNTDTLC

Target ORF information:

RefSeq Version	XM_005165231.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165231.3

ORF Insert Sequence:

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG TGTCCTTCTA TAGCGCGGCG 
CCGCTCACCT CGGCGGGCAT CATTCCCATC ATGCAGTCGT TGTGTCCGGA TGGTCAGCGG 
GATGAGTTTG GCTTCCTGCA GTATAAAAAC TCCACTGTGA CTCAATTGCT GGAAAGGATA 
AGTGAAGTTG TGGAGCAGAA TCACTTGTTT ACATCAGACA GGCCCAGTCT GGGAGAGGAG 
CTGGAGTCTC TGCAGCAGCA CCTCGAAAGT CTGAGCTCAG CACAAGGCCC TCTAGAGAGC 
CACTACAACA CCAGCCACGG TTTCACTGTG GGAAGCGTCC TCAGGAACCA GTCGATGTTC 
CAGCAGTTTC TGATGCGAAA TCTCTCCCTG CCGAACGACA CGTCCAGCCT GCTGCTCTCC 
TCCCCCGTCA ATCTAAAAGA GGTATATAAT CTCATCTTTG GGACGTATCC CAAAGCGGGA 
TCAAAGGGAG GAGAAAGAGA TGAAGGACAG CACCAACTCA ACAGTCCTGG AGAGAAGCTT 
GTACAGCTGG AGCAACTACT TGGAGGCTGG CACAGTGTAG ACGGGGGTCT GATTCAGAAG 
GCACTGAGAG ATCCCACAAA AGCTGCTCAC CGTCAAGCTC TTCTCAAAGT CTTCTCTCAG 
GCGTTGGGCA TCGCTGGCGG TGTAGGAGGT CAAAAATTCG ATCCTCAAGC ACTGCGTGAG 
ATGGAGGGAG TTCTGTTGAC TGGTGCAGTG CTTGAAAGCC TTACCTGTGG GGAAGGAGGG 
AACAGTGAGA TTGGCAAAAT CCTATTGGTC CCTGAGAAAC AACAGGCGGT TTTACAGGCT 
TACCGCACGA CTGTTTGTGG TTTAGGGGCG GGGCAGAGGG CGGAGCTTTT CAGACAGTTG 
AGTGAAGAAC TGAGAGATCA GATTGACACA CAGAGAGTCT TTGATCAGTT GCGTCTTGAG 
CAGTCTAATA TGTCTGGTCT GCTGGATCAG TCCCGTCTCG GGGCTCTCCT GAAGAACCTG 
GCTGATGTGG AGCGGCTGCT GAGGGACGTG GATCTACTGT CAGGTCTGGC CAGACTGCTG 
CCGAAAGGGG CCTGTGCGGG TCACCAGCCT CCTCCTCAGG CCAACACCAC CTCCTGGAGC 
TCCAACTCTA CCACATGGGG TCCCAATACA ACTGACAGTT CTGTGGAGGA AGGAGAAGGA 
GCTTCTGAGA GCGAGGCAGA GGCTGAAAAC CCCAAATCAC AGTTTTCTGC ATTTGTTCAG 
CTGTGGGCAG GACTTCAACC CATACTTTGT GGGAATAATA GGATTTTCGA ACCAGAAGCG 
CTGAAACAAG GAAATATGAG TTCTCTGGGC TTCACCAGCA AAGAACAGAG AAATTTAGGA 
TTACTTGTTC ATCTCATGAC TACAAATCCC AAAATCCTCT ACTCTCCCAT TGACTCAGAG 
GTGGACAAGG TCATACAAAA GGCCAATGAG ACCTTTGCTT TTGTGGGGAA TGTGACTCAC 
TATGCCCGTG TGTGGCTCAA CATCTCTGCT GAATTAAGGG CCTTTTTAGA GGAGGGTAAA 
TTGCAAAATC ACCTGATGTG GCTCCAGCAG TTTATTACCG ACCTGAAGAG GCACCATGAG 
CTGCTGAACG TATCAGACAG TGAGCTGATG AACAGTCTGC TGGAGGAGAA CTTCACCCTG 
CCAAACACAA CCACCTTACT GGAGCAGCTG CACACCATTG ATAATGCTGC TTGTGGATGG 
ACTAACTTCA TGTCAAAGGT CAGTGTGGAT ATCTTCAGAG GCTTCCCTGA TGAAGAAAGC 
ATTGTCAATT ACACACTGAA CCAGGCCTAC CACGACAACG TCTCAGTCTT TGCAAGTGTG 
ATTTTCCAGA CCAATAAAGA TGGATCCCTG CCACCCCATG TTATGTATAA AATTCGACAG 
AACTCGAGCT TTACCGAGAA AACCAATGAA ATCCGACGTG CTTATTGGCG CCCAGGGCCA 
AACACAGGCG GCAAATTCTA CTTCCTTTAT GGCTTTGTGT GGATCCAAGA TATGATTGAA 
CGGGCAATCA TCAACACCTT TGTTGGCCAC GATGTTGTGG AGCCTGGAAA TTACGTGCAG 
ATGTTTCCTT ATCCGTGCTA CACCAGAGAC GATTTTCTCT TTGTTATCGA GCACATGATG 
CCTCTGTGTA TGGTCATATC CTGGGTCTAC TCTGTGGCCA TGATGATCCA GCACATCGTA 
GCAGAGAAGG AGCAAAGACT GAAAGAGGTA ATGAAGATGA TGGGCTTGAA TAACGCAGTG 
CACTGGGTGG CCTGGTTCAT CACAGGCTTC GTGCAGCTTT CCATCTCAGT GACGGCCCTC 
ACTGCCATAC TGAAATACGG GAAAGTCCTC CTTCACAGCG ATCCCTTCAT CATATGGCTT 
TTTCTTACAA TCTACGCTGT CGCAACCATC ATGTTCTGTT TCCTGGTGTC GGTGTTGTAC 
TCTAAAGCCA AACTGGCCTC GGCATGTGGA GGAATCATCT ACTTCCTGAG TTATGTGCCC 
TACATGTATG TGGCCATAAG AGAAGAGGTG GCTCACGATA AGATCACTGC CTTTGAGAAG 
TGCATAGCGT CTTTGATGTC TACCACAGCG TTCGGTCTGG GATCCAAGTA CTTTGCCCTG 
TATGAAGTGG CTGGCGTGGG AATTCAGTGG CGCACTATTA ACCAGTCACC TGTAGAGGGC 
GATGACTTCA ATCTACTGCT GTCTATGGTC ATGCTCACTA TTGACGCCAT TGTATATGGT 
GTGTTGACCT GGTATATAGA AGCCGTCCAT CCAGGGATGT ATGGACTGCC TCGGCCATGG 
TATTTCCCCT TGCAGAAGTC CTATTGGTTA GGTAGTGGCC GCATAGAGAC GTGGGAGTGG 
CCGTGGGGTG GAGGCACCAG GCTGAGTGTT ATGGAAGAGG ACCAGGCTTG TGCCATGGAG 
CATAGACGCT CAGAGGAAAC CCGTGGAATT GAAGAAGAGC CCAATCATTT GCCGCTGGTG 
GTGTGCATCG ACAAACTGAC CAAAGTCTAC AAGACGGGCA GCAAACTGGC TCTAAACAAG 
CTGAGCCTGA ACCTGCATGA GAACCAGGTT GTCTCTTTCT TGGGACACAA TGGGGCTGGC 
AAGACCACCA CTATGTCCAT TCTCACGGGA CTCTTTCCAC CCACCTCTGG CTCTGCCACC 
ATATACGGCC ATGACATCCG CACAGAGATG GAGCGCATCA GGCAGAACTT GGGCATGTGT 
CCCCAGCACA ACGTCCTGTT TGATAAGCTC AGTGTGGAGG AACATCTGTG GTTCTACTCA 
CGGCTGAAAG GCATGGCAGA GGAAGATATT CGCAAGGAGA TGGACAAGAT GATAGAGGAT 
CTCGAATTAT CTAATAAACG TCACAGTCTG GTTCAAACCC TGTCAGGAGG GATGAAAAGG 
AAGCTGTCAG TGGCCATTGC TTTTGTAGGT GGGTCTCGGG CAGTGATTTT GGATGAACCA 
ACAGCAGGAG TAGACCCATA TGCCCGTCGA GCGATCTGGG ACCTCATCCT GAAATATAAG 
CAAGGTCGCA CCATCCTGCT GTCCACTCAC CACATGGATG AGGCTGATCT GCTGGGTGAC 
CGCATTGCCA TCATCTCTCA TGGCAAGCTC AAGTGCTGCG GTTCTCCTCT GTTCCTCAAG 
AGCACATATG GAGACGGATA CAAACTCACA CTTGTTAAAA AACAGAGTGA CTCACACACT 
GCAGATCAGT CAAGCCAGTC TCCGTCCTCT TCGATATCTC CATGTTCAGA AAACAGAGTA 
ACTCAGTTCA TCCGTCAGTA TGTTGCTTCC TGCCTGCTGG TGTCCGACTC CAACACTGAA 
CTCTCTTATG TCCTACCATC AGAGGCTGTC AAGAAAGGCT GCTTTGAGAG ACTCTTCCAG 
GCTCTGGAGC AGAGTTTAGA CAGCTTGGCC TTGACCAGCT TTGGCGTGAT GGACACCACA 
TTGGAGGAGG TGTTTCTAAA GGTGTCAGAA GAAGATCTGT CGCTGGAAAA CAGCGATGCA 
GACATGAAGG ACTCTCCAGG CGGAGCATCA GCCGGGAAGC CGTCAGGTCT GTTAGGAGGG 
CCTCAGTGTG AAGGAGCTCC AGCCGCTGCT GTGATGAGGC CAGAGGTGGA GCTCAGCAAC 
CTGATGATGT GCTCCAGGCT GAGCCCAAGC CAAGCATCCC TGCAGTCCGG CTCTTCTCTG 
GGCTCAGTGA GAGGAGATGA AGGGGGTCCC TATTCTGAGT TCTATGGAGA CTATTGCCCA 
CTGTTTGACA ACGGTCAGGA TCTGGACTCT GCCAGTCTCA GAGATGAAGA GTCATCTCCT 
CAGCCCACTG TACTCGAGGG CCAGGGCAGC TTCAAGCTCG ATGGCTGGTG GCTTAAACTC 
CGCCAGTTTC ATGGGCTGAT CGTGAAAAGG TTTCACTGTG CCAAGAGGAA CACCAAGGGC 
ATTTTCTCTC AGATTCTGCT GCCTGCTTTC TTCGTATGTG TGGCAATGAC TGTGGCTCTG 
TCTGTGCCTG AGATTGGTGA CCTTCCTCCC CTGATCCTGT CTCCATCTCA ATATCACAAC 
TACACACAGC CTCGAGGAAA CTTCATCCCC TACGCCAATG AAGACAGATC TCAATACAGG 
ACTAAGTTTT CTCCAGACGC AGGCCCACAG AGGATTGTTA ACACCCTCCG TCTGCCATCT 
GGAGTCGGTG CGACTTGTAT CCTGAAGACT CCCTTTAACA GCACCCTGGA CCAACTGGCC 
CAAACTCTCA ATCCTTATGC CAACAACTCT AAAACCCTGG CAGCCCGCTA CTTTGACTCT 
ATGTGCCTGG ACTCCTTCAC GCAGGGTGTG CCTTTGTCGA ATTTCGTTCC CCCTCCGCCC 
TCTCCTGCCC CCTCAGACGA CCCGGAGGCT CATTTTGAAG ACGGCCTGTG GAACTACACT 
GCAGCTCCTA CTACTGTCAG AGAGATGGCC ACGTCTCCCC CGACTCTGCC CTTGAGTATC 
CATGAGCCGG TGCGCTGTAT CTGCTCTATG CAGGGCACAG GCTTCTCCTG CCCCAGTGGA 
GTTGGGGGTC GACCTCCTCT GATGAAAGTG GTGACCGGAG ACATCCTGGT GGACATTACA 
GGTCGCAACG TGTCTGAATA TCTCCTGTAC ACGTCTGATC GGCTCAGGTT ACACAGATAT 
GGAGGCTTTA CTGTTGGAAA CATTCAGAAA TCTGTCCCAG CATCCTTCGG GAGAAAAACA 
CCACCTATGG TGCGCAAAAT AGCAGTGAGG CGATCCTCAC AGGTGCTGTA TAATAACAAA 
GGCTATCACA GTATGCCAAC ATATCTAAAT GTCCTCAACA ATGCCATTCT CAGAGCCAAT 
CTGCCCAGTA GTAAAGGAAA CCCTTCTGCA TACGGCATTA CAGTGACCAA TCATCCCATG 
AACAGAACCA GTGCCAGTCT TTCTCTGGAT TATCTGCTCC AGGGCACTGA TGTGGTTATT 
GCCATCTTCA TCATTGTAGC CATGTCTTTC GTGCCTGCGA GCTTTGTGGT CTTTCTGGTA 
GCGGAAAAAT CCACCAAAGC CAAACACCTG CAGTTTGTCA GCGGATGCGA CCCTGTCACG 
TACTGGCTAG CAAACTACAT ATGGGACATG CTGAACTACC TGGTTCCAGC TACATGCTGT 
GTCCTAATTT TGTTTGTGTT CGACCTTCCT GCGTACACGT CACCTACAAA TTTCCCAGCA 
GTGCTCTCTC TGTTTCTGCT TTATGGCTGG TCTATAACCC CCATCATGTA CCCAGCCTCC 
TTCTGGTTTG AGGTGCCCAG CACAGCTTAT GTTTTCCTCA TAGTCATCAA TCTCTTCATT 
GGCATCACAG CCACGGTGGC CACTTTCCTC CTCCAGCTGT TTGAGCGCGA CAAGGACCTG 
AAGTTGGTAA ACAGCTACCT GAAGTCATGT TTTCTCATCT TCCCCAACTA TAACCTGGGT 
CACGGATTGA TGGAGATGGC CTATAATGAG TATATCAATG AATATTACGC CAAGATAGGC 
CAGTTTGATA AAGTGAAGTC TCCATTTGAA TGGGACATTG TGACGCGGGG ACTGGTAGCA 
ATGACCATTG AGGGCTTCGT CGGCTTTCTC ATTACAATTC TGTGCCAGTA CAACTTCCTC 
AGGAAAGCCC AGAGAGTGCC TGTCAACAGT CAGCCAGTGG AGGACGATGA TGTTGATGTG 
GCCTGTGAAA GACGCCGAGT TCTGAGGGGA GACGCTGACA ACGACATGCT GAAGATCGAC 
AACCTAACCA AGGTGTACAA GTCTCGTAAG ATGGGCCGGA TCCTGGCAGT GGACCGATTG 
TGTCTGGGGG TCCGTCCTGG TGAATGCTTC GGTCTGCTGG GAGTGAATGG GGCAGGAAAG 
ACCACCACCT TCAAAATGTT GACAGGAGAT GAGAGCACAA CAGGGGGAGA AGCCTTTATC 
CAGGAACACA GTATCTTGCG GGAGCTGTTA CGTGTGCAGC AGAGCATCGG TTACTGCCCT 
CAGTTTGACG CTCTGTTTGA TGATCTGACG GCTCGAGAGC ATCTGGAGCT CTACACGCGC 
CTCCGCGGCA TCCCATGGAA AGATGAGGAG CGGGTGGTTC AGTGGGCTTT GGAGAAGTTG 
GAGTTGTCCA AGTATGCCGA CAAGCCTGCT GGCACCTACA GCGGAGGCAA CAAACGCAAA 
CTCTCTACAG CCATTGCTCT GATTGGATAC CCATCGCTCA TCTTTTTGGA TGAGCCCACC 
ACAGGTATGG ACCCAAAGGC TCGCCGTTTC TTATGGAACC TAATTCTTGA CATCATCAAG 
ACGGGCCGCT CAGTAGTGTT GACGTCACAC AGTATGGAGG AGTGTGAAGC GCTGTGCACT 
AGACTGGGCA TCATGGTTAA TGGCAGGTTC AAGTGTCTAG GAAGCATCCA GCATCTCAAA 
AACAGATTCG GAGATGGATA CATGATAACA GTACGCACTA AGACCACCGC TAGCGTTAAA 
GAAGTGATTC GGTTTTTTAA CAGAAATTTT CCTGAGGCAA TTCTAAAGGA GCGACACCAC 
ACAAAGATTC AGTACCAGCT GAAGTCAGAG AACATCTCTC TGGCTCAGGT CTTCAGCAAA 
ATGGAGCAGG TGGTGGAGGT GCTGAGCATC GAGGATTATT CCGTCAGCCA AACCACACTG 
GATAACGTCT TTGTTAACTT TGCAAAAAAG CAAAGCGACA ACTTAGAGCA GCAAGAAAGT 
TCTCCATCTA GTGGTGGCCA GTCGCCACTG CAGCATCTGC TCAGCCTCCT CAGGCCTCGT 
CCTGCAAACA CTGAGCTCAA CGCCCTCGTG AGTGAAGAGC CTGAGGAACT GGAGAGTGAT 
GATGACGAAG GACTGATCAG CTTTGAGGAA GAGAGGGTGC AGTTGTCCTT TAATACAGAC 
ACTCTGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

abca2 ( XM_005165231.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165288.1 Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X3, mRNA.

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CloneID	ODa10383
Clone ID Related Accession (Same CDS sequence)	XM_005165231.3 , XM_005165231.4
Accession Version	XM_005165231.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ATP-binding cassette sub-family A member 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165231.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG TGTCCTTCTA TAGCGCGGCG 
CCGCTCACCT CGGCGGGCAT CATTCCCATC ATGCAGTCGT TGTGTCCGGA TGGTCAGCGG 
GATGAGTTTG GCTTCCTGCA GTATAAAAAC TCCACTGTGA CTCAATTGCT GGAAAGGATA 
AGTGAAGTTG TGGAGCAGAA TCACTTGTTT ACATCAGACA GGCCCAGTCT GGGAGAGGAG 
CTGGAGTCTC TGCAGCAGCA CCTCGAAAGT CTGAGCTCAG CACAAGGCCC TCTAGAGAGC 
CACTACAACA CCAGCCACGG TTTCACTGTG GGAAGCGTCC TCAGGAACCA GTCGATGTTC 
CAGCAGTTTC TGATGCGAAA TCTCTCCCTG CCGAACGACA CGTCCAGCCT GCTGCTCTCC 
TCCCCCGTCA ATCTAAAAGA GGTATATAAT CTCATCTTTG GGACGTATCC CAAAGCGGGA 
TCAAAGGGAG GAGAAAGAGA TGAAGGACAG CACCAACTCA ACAGTCCTGG AGAGAAGCTT 
GTACAGCTGG AGCAACTACT TGGAGGCTGG CACAGTGTAG ACGGGGGTCT GATTCAGAAG 
GCACTGAGAG ATCCCACAAA AGCTGCTCAC CGTCAAGCTC TTCTCAAAGT CTTCTCTCAG 
GCGTTGGGCA TCGCTGGCGG TGTAGGAGGT CAAAAATTCG ATCCTCAAGC ACTGCGTGAG 
ATGGAGGGAG TTCTGTTGAC TGGTGCAGTG CTTGAAAGCC TTACCTGTGG GGAAGGAGGG 
AACAGTGAGA TTGGCAAAAT CCTATTGGTC CCTGAGAAAC AACAGGCGGT TTTACAGGCT 
TACCGCACGA CTGTTTGTGG TTTAGGGGCG GGGCAGAGGG CGGAGCTTTT CAGACAGTTG 
AGTGAAGAAC TGAGAGATCA GATTGACACA CAGAGAGTCT TTGATCAGTT GCGTCTTGAG 
CAGTCTAATA TGTCTGGTCT GCTGGATCAG TCCCGTCTCG GGGCTCTCCT GAAGAACCTG 
GCTGATGTGG AGCGGCTGCT GAGGGACGTG GATCTACTGT CAGGTCTGGC CAGACTGCTG 
CCGAAAGGGG CCTGTGCGGG TCACCAGCCT CCTCCTCAGG CCAACACCAC CTCCTGGAGC 
TCCAACTCTA CCACATGGGG TCCCAATACA ACTGACAGTT CTGTGGAGGA AGGAGAAGGA 
GCTTCTGAGA GCGAGGCAGA GGCTGAAAAC CCCAAATCAC AGTTTTCTGC ATTTGTTCAG 
CTGTGGGCAG GACTTCAACC CATACTTTGT GGGAATAATA GGATTTTCGA ACCAGAAGCG 
CTGAAACAAG GAAATATGAG TTCTCTGGGC TTCACCAGCA AAGAACAGAG AAATTTAGGA 
TTACTTGTTC ATCTCATGAC TACAAATCCC AAAATCCTCT ACTCTCCCAT TGACTCAGAG 
GTGGACAAGG TCATACAAAA GGCCAATGAG ACCTTTGCTT TTGTGGGGAA TGTGACTCAC 
TATGCCCGTG TGTGGCTCAA CATCTCTGCT GAATTAAGGG CCTTTTTAGA GGAGGGTAAA 
TTGCAAAATC ACCTGATGTG GCTCCAGCAG TTTATTACCG ACCTGAAGAG GCACCATGAG 
CTGCTGAACG TATCAGACAG TGAGCTGATG AACAGTCTGC TGGAGGAGAA CTTCACCCTG 
CCAAACACAA CCACCTTACT GGAGCAGCTG CACACCATTG ATAATGCTGC TTGTGGATGG 
ACTAACTTCA TGTCAAAGGT CAGTGTGGAT ATCTTCAGAG GCTTCCCTGA TGAAGAAAGC 
ATTGTCAATT ACACACTGAA CCAGGCCTAC CACGACAACG TCTCAGTCTT TGCAAGTGTG 
ATTTTCCAGA CCAATAAAGA TGGATCCCTG CCACCCCATG TTATGTATAA AATTCGACAG 
AACTCGAGCT TTACCGAGAA AACCAATGAA ATCCGACGTG CTTATTGGCG CCCAGGGCCA 
AACACAGGCG GCAAATTCTA CTTCCTTTAT GGCTTTGTGT GGATCCAAGA TATGATTGAA 
CGGGCAATCA TCAACACCTT TGTTGGCCAC GATGTTGTGG AGCCTGGAAA TTACGTGCAG 
ATGTTTCCTT ATCCGTGCTA CACCAGAGAC GATTTTCTCT TTGTTATCGA GCACATGATG 
CCTCTGTGTA TGGTCATATC CTGGGTCTAC TCTGTGGCCA TGATGATCCA GCACATCGTA 
GCAGAGAAGG AGCAAAGACT GAAAGAGGTA ATGAAGATGA TGGGCTTGAA TAACGCAGTG 
CACTGGGTGG CCTGGTTCAT CACAGGCTTC GTGCAGCTTT CCATCTCAGT GACGGCCCTC 
ACTGCCATAC TGAAATACGG GAAAGTCCTC CTTCACAGCG ATCCCTTCAT CATATGGCTT 
TTTCTTACAA TCTACGCTGT CGCAACCATC ATGTTCTGTT TCCTGGTGTC GGTGTTGTAC 
TCTAAAGCCA AACTGGCCTC GGCATGTGGA GGAATCATCT ACTTCCTGAG TTATGTGCCC 
TACATGTATG TGGCCATAAG AGAAGAGGTG GCTCACGATA AGATCACTGC CTTTGAGAAG 
TGCATAGCGT CTTTGATGTC TACCACAGCG TTCGGTCTGG GATCCAAGTA CTTTGCCCTG 
TATGAAGTGG CTGGCGTGGG AATTCAGTGG CGCACTATTA ACCAGTCACC TGTAGAGGGC 
GATGACTTCA ATCTACTGCT GTCTATGGTC ATGCTCACTA TTGACGCCAT TGTATATGGT 
GTGTTGACCT GGTATATAGA AGCCGTCCAT CCAGGGATGT ATGGACTGCC TCGGCCATGG 
TATTTCCCCT TGCAGAAGTC CTATTGGTTA GGTAGTGGCC GCATAGAGAC GTGGGAGTGG 
CCGTGGGGTG GAGGCACCAG GCTGAGTGTT ATGGAAGAGG ACCAGGCTTG TGCCATGGAG 
CATAGACGCT CAGAGGAAAC CCGTGGAATT GAAGAAGAGC CCAATCATTT GCCGCTGGTG 
GTGTGCATCG ACAAACTGAC CAAAGTCTAC AAGACGGGCA GCAAACTGGC TCTAAACAAG 
CTGAGCCTGA ACCTGCATGA GAACCAGGTT GTCTCTTTCT TGGGACACAA TGGGGCTGGC 
AAGACCACCA CTATGTCCAT TCTCACGGGA CTCTTTCCAC CCACCTCTGG CTCTGCCACC 
ATATACGGCC ATGACATCCG CACAGAGATG GAGCGCATCA GGCAGAACTT GGGCATGTGT 
CCCCAGCACA ACGTCCTGTT TGATAAGCTC AGTGTGGAGG AACATCTGTG GTTCTACTCA 
CGGCTGAAAG GCATGGCAGA GGAAGATATT CGCAAGGAGA TGGACAAGAT GATAGAGGAT 
CTCGAATTAT CTAATAAACG TCACAGTCTG GTTCAAACCC TGTCAGGAGG GATGAAAAGG 
AAGCTGTCAG TGGCCATTGC TTTTGTAGGT GGGTCTCGGG CAGTGATTTT GGATGAACCA 
ACAGCAGGAG TAGACCCATA TGCCCGTCGA GCGATCTGGG ACCTCATCCT GAAATATAAG 
CAAGGTCGCA CCATCCTGCT GTCCACTCAC CACATGGATG AGGCTGATCT GCTGGGTGAC 
CGCATTGCCA TCATCTCTCA TGGCAAGCTC AAGTGCTGCG GTTCTCCTCT GTTCCTCAAG 
AGCACATATG GAGACGGATA CAAACTCACA CTTGTTAAAA AACAGAGTGA CTCACACACT 
GCAGATCAGT CAAGCCAGTC TCCGTCCTCT TCGATATCTC CATGTTCAGA AAACAGAGTA 
ACTCAGTTCA TCCGTCAGTA TGTTGCTTCC TGCCTGCTGG TGTCCGACTC CAACACTGAA 
CTCTCTTATG TCCTACCATC AGAGGCTGTC AAGAAAGGCT GCTTTGAGAG ACTCTTCCAG 
GCTCTGGAGC AGAGTTTAGA CAGCTTGGCC TTGACCAGCT TTGGCGTGAT GGACACCACA 
TTGGAGGAGG TGTTTCTAAA GGTGTCAGAA GAAGATCTGT CGCTGGAAAA CAGCGATGCA 
GACATGAAGG ACTCTCCAGG CGGAGCATCA GCCGGGAAGC CGTCAGGTCT GTTAGGAGGG 
CCTCAGTGTG AAGGAGCTCC AGCCGCTGCT GTGATGAGGC CAGAGGTGGA GCTCAGCAAC 
CTGATGATGT GCTCCAGGCT GAGCCCAAGC CAAGCATCCC TGCAGTCCGG CTCTTCTCTG 
GGCTCAGTGA GAGGAGATGA AGGGGGTCCC TATTCTGAGT TCTATGGAGA CTATTGCCCA 
CTGTTTGACA ACGGTCAGGA TCTGGACTCT GCCAGTCTCA GAGATGAAGA GTCATCTCCT 
CAGCCCACTG TACTCGAGGG CCAGGGCAGC TTCAAGCTCG ATGGCTGGTG GCTTAAACTC 
CGCCAGTTTC ATGGGCTGAT CGTGAAAAGG TTTCACTGTG CCAAGAGGAA CACCAAGGGC 
ATTTTCTCTC AGATTCTGCT GCCTGCTTTC TTCGTATGTG TGGCAATGAC TGTGGCTCTG 
TCTGTGCCTG AGATTGGTGA CCTTCCTCCC CTGATCCTGT CTCCATCTCA ATATCACAAC 
TACACACAGC CTCGAGGAAA CTTCATCCCC TACGCCAATG AAGACAGATC TCAATACAGG 
ACTAAGTTTT CTCCAGACGC AGGCCCACAG AGGATTGTTA ACACCCTCCG TCTGCCATCT 
GGAGTCGGTG CGACTTGTAT CCTGAAGACT CCCTTTAACA GCACCCTGGA CCAACTGGCC 
CAAACTCTCA ATCCTTATGC CAACAACTCT AAAACCCTGG CAGCCCGCTA CTTTGACTCT 
ATGTGCCTGG ACTCCTTCAC GCAGGGTGTG CCTTTGTCGA ATTTCGTTCC CCCTCCGCCC 
TCTCCTGCCC CCTCAGACGA CCCGGAGGCT CATTTTGAAG ACGGCCTGTG GAACTACACT 
GCAGCTCCTA CTACTGTCAG AGAGATGGCC ACGTCTCCCC CGACTCTGCC CTTGAGTATC 
CATGAGCCGG TGCGCTGTAT CTGCTCTATG CAGGGCACAG GCTTCTCCTG CCCCAGTGGA 
GTTGGGGGTC GACCTCCTCT GATGAAAGTG GTGACCGGAG ACATCCTGGT GGACATTACA 
GGTCGCAACG TGTCTGAATA TCTCCTGTAC ACGTCTGATC GGCTCAGGTT ACACAGATAT 
GGAGGCTTTA CTGTTGGAAA CATTCAGAAA TCTGTCCCAG CATCCTTCGG GAGAAAAACA 
CCACCTATGG TGCGCAAAAT AGCAGTGAGG CGATCCTCAC AGGTGCTGTA TAATAACAAA 
GGCTATCACA GTATGCCAAC ATATCTAAAT GTCCTCAACA ATGCCATTCT CAGAGCCAAT 
CTGCCCAGTA GTAAAGGAAA CCCTTCTGCA TACGGCATTA CAGTGACCAA TCATCCCATG 
AACAGAACCA GTGCCAGTCT TTCTCTGGAT TATCTGCTCC AGGGCACTGA TGTGGTTATT 
GCCATCTTCA TCATTGTAGC CATGTCTTTC GTGCCTGCGA GCTTTGTGGT CTTTCTGGTA 
GCGGAAAAAT CCACCAAAGC CAAACACCTG CAGTTTGTCA GCGGATGCGA CCCTGTCACG 
TACTGGCTAG CAAACTACAT ATGGGACATG CTGAACTACC TGGTTCCAGC TACATGCTGT 
GTCCTAATTT TGTTTGTGTT CGACCTTCCT GCGTACACGT CACCTACAAA TTTCCCAGCA 
GTGCTCTCTC TGTTTCTGCT TTATGGCTGG TCTATAACCC CCATCATGTA CCCAGCCTCC 
TTCTGGTTTG AGGTGCCCAG CACAGCTTAT GTTTTCCTCA TAGTCATCAA TCTCTTCATT 
GGCATCACAG CCACGGTGGC CACTTTCCTC CTCCAGCTGT TTGAGCGCGA CAAGGACCTG 
AAGTTGGTAA ACAGCTACCT GAAGTCATGT TTTCTCATCT TCCCCAACTA TAACCTGGGT 
CACGGATTGA TGGAGATGGC CTATAATGAG TATATCAATG AATATTACGC CAAGATAGGC 
CAGTTTGATA AAGTGAAGTC TCCATTTGAA TGGGACATTG TGACGCGGGG ACTGGTAGCA 
ATGACCATTG AGGGCTTCGT CGGCTTTCTC ATTACAATTC TGTGCCAGTA CAACTTCCTC 
AGGAAAGCCC AGAGAGTGCC TGTCAACAGT CAGCCAGTGG AGGACGATGA TGTTGATGTG 
GCCTGTGAAA GACGCCGAGT TCTGAGGGGA GACGCTGACA ACGACATGCT GAAGATCGAC 
AACCTAACCA AGGTGTACAA GTCTCGTAAG ATGGGCCGGA TCCTGGCAGT GGACCGATTG 
TGTCTGGGGG TCCGTCCTGG TGAATGCTTC GGTCTGCTGG GAGTGAATGG GGCAGGAAAG 
ACCACCACCT TCAAAATGTT GACAGGAGAT GAGAGCACAA CAGGGGGAGA AGCCTTTATC 
CAGGAACACA GTATCTTGCG GGAGCTGTTA CGTGTGCAGC AGAGCATCGG TTACTGCCCT 
CAGTTTGACG CTCTGTTTGA TGATCTGACG GCTCGAGAGC ATCTGGAGCT CTACACGCGC 
CTCCGCGGCA TCCCATGGAA AGATGAGGAG CGGGTGGTTC AGTGGGCTTT GGAGAAGTTG 
GAGTTGTCCA AGTATGCCGA CAAGCCTGCT GGCACCTACA GCGGAGGCAA CAAACGCAAA 
CTCTCTACAG CCATTGCTCT GATTGGATAC CCATCGCTCA TCTTTTTGGA TGAGCCCACC 
ACAGGTATGG ACCCAAAGGC TCGCCGTTTC TTATGGAACC TAATTCTTGA CATCATCAAG 
ACGGGCCGCT CAGTAGTGTT GACGTCACAC AGTATGGAGG AGTGTGAAGC GCTGTGCACT 
AGACTGGGCA TCATGGTTAA TGGCAGGTTC AAGTGTCTAG GAAGCATCCA GCATCTCAAA 
AACAGATTCG GAGATGGATA CATGATAACA GTACGCACTA AGACCACCGC TAGCGTTAAA 
GAAGTGATTC GGTTTTTTAA CAGAAATTTT CCTGAGGCAA TTCTAAAGGA GCGACACCAC 
ACAAAGATTC AGTACCAGCT GAAGTCAGAG AACATCTCTC TGGCTCAGGT CTTCAGCAAA 
ATGGAGCAGG TGGTGGAGGT GCTGAGCATC GAGGATTATT CCGTCAGCCA AACCACACTG 
GATAACGTCT TTGTTAACTT TGCAAAAAAG CAAAGCGACA ACTTAGAGCA GCAAGAAAGT 
TCTCCATCTA GTGGTGGCCA GTCGCCACTG CAGCATCTGC TCAGCCTCCT CAGGCCTCGT 
CCTGCAAACA CTGAGCTCAA CGCCCTCGTG AGTGAAGAGC CTGAGGAACT GGAGAGTGAT 
GATGACGAAG GACTGATCAG CTTTGAGGAA GAGAGGGTGC AGTTGTCCTT TAATACAGAC 
ACTCTGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165288.1
CDS	554..8065
Translation	MGFLHQLHLLLWKNVTLKRRGPWVLAFEIFIPLVLFFILLGLRQKKPAIPVKEVSFYSAAPLTSAGIIPIMQSLCPDGQRDEFGFLQYKNSTVTQLLERISEVVEQNHLFTSDRPSLGEELESLQQHLESLSSAQGPLESHYNTSHGFTVGSVLRNQSMFQQFLMRNLSLPNDTSSLLLSSPVNLKEVYNLIFGTYPKAGSKGGERDEGQHQLNSPGEKLVQLEQLLGGWHSVDGGLIQKALRDPTKAAHRQALLKVFSQALGIAGGVGGQKFDPQALREMEGVLLTGAVLESLTCGEGGNSEIGKILLVPEKQQAVLQAYRTTVCGLGAGQRAELFRQLSEELRDQIDTQRVFDQLRLEQSNMSGLLDQSRLGALLKNLADVERLLRDVDLLSGLARLLPKGACAGHQPPPQANTTSWSSNSTTWGPNTTDSSVEEGEGASESEAEAENPKSQFSAFVQLWAGLQPILCGNNRIFEPEALKQGNMSSLGFTSKEQRNLGLLVHLMTTNPKILYSPIDSEVDKVIQKANETFAFVGNVTHYARVWLNISAELRAFLEEGKLQNHLMWLQQFITDLKRHHELLNVSDSELMNSLLEENFTLPNTTTLLEQLHTIDNAACGWTNFMSKVSVDIFRGFPDEESIVNYTLNQAYHDNVSVFASVIFQTNKDGSLPPHVMYKIRQNSSFTEKTNEIRRAYWRPGPNTGGKFYFLYGFVWIQDMIERAIINTFVGHDVVEPGNYVQMFPYPCYTRDDFLFVIEHMMPLCMVISWVYSVAMMIQHIVAEKEQRLKEVMKMMGLNNAVHWVAWFITGFVQLSISVTALTAILKYGKVLLHSDPFIIWLFLTIYAVATIMFCFLVSVLYSKAKLASACGGIIYFLSYVPYMYVAIREEVAHDKITAFEKCIASLMSTTAFGLGSKYFALYEVAGVGIQWRTINQSPVEGDDFNLLLSMVMLTIDAIVYGVLTWYIEAVHPGMYGLPRPWYFPLQKSYWLGSGRIETWEWPWGGGTRLSVMEEDQACAMEHRRSEETRGIEEEPNHLPLVVCIDKLTKVYKTGSKLALNKLSLNLHENQVVSFLGHNGAGKTTTMSILTGLFPPTSGSATIYGHDIRTEMERIRQNLGMCPQHNVLFDKLSVEEHLWFYSRLKGMAEEDIRKEMDKMIEDLELSNKRHSLVQTLSGGMKRKLSVAIAFVGGSRAVILDEPTAGVDPYARRAIWDLILKYKQGRTILLSTHHMDEADLLGDRIAIISHGKLKCCGSPLFLKSTYGDGYKLTLVKKQSDSHTADQSSQSPSSSISPCSENRVTQFIRQYVASCLLVSDSNTELSYVLPSEAVKKGCFERLFQALEQSLDSLALTSFGVMDTTLEEVFLKVSEEDLSLENSDADMKDSPGGASAGKPSGLLGGPQCEGAPAAAVMRPEVELSNLMMCSRLSPSQASLQSGSSLGSVRGDEGGPYSEFYGDYCPLFDNGQDLDSASLRDEESSPQPTVLEGQGSFKLDGWWLKLRQFHGLIVKRFHCAKRNTKGIFSQILLPAFFVCVAMTVALSVPEIGDLPPLILSPSQYHNYTQPRGNFIPYANEDRSQYRTKFSPDAGPQRIVNTLRLPSGVGATCILKTPFNSTLDQLAQTLNPYANNSKTLAARYFDSMCLDSFTQGVPLSNFVPPPPSPAPSDDPEAHFEDGLWNYTAAPTTVREMATSPPTLPLSIHEPVRCICSMQGTGFSCPSGVGGRPPLMKVVTGDILVDITGRNVSEYLLYTSDRLRLHRYGGFTVGNIQKSVPASFGRKTPPMVRKIAVRRSSQVLYNNKGYHSMPTYLNVLNNAILRANLPSSKGNPSAYGITVTNHPMNRTSASLSLDYLLQGTDVVIAIFIIVAMSFVPASFVVFLVAEKSTKAKHLQFVSGCDPVTYWLANYIWDMLNYLVPATCCVLILFVFDLPAYTSPTNFPAVLSLFLLYGWSITPIMYPASFWFEVPSTAYVFLIVINLFIGITATVATFLLQLFERDKDLKLVNSYLKSCFLIFPNYNLGHGLMEMAYNEYINEYYAKIGQFDKVKSPFEWDIVTRGLVAMTIEGFVGFLITILCQYNFLRKAQRVPVNSQPVEDDDVDVACERRRVLRGDADNDMLKIDNLTKVYKSRKMGRILAVDRLCLGVRPGECFGLLGVNGAGKTTTFKMLTGDESTTGGEAFIQEHSILRELLRVQQSIGYCPQFDALFDDLTAREHLELYTRLRGIPWKDEERVVQWALEKLELSKYADKPAGTYSGGNKRKLSTAIALIGYPSLIFLDEPTTGMDPKARRFLWNLILDIIKTGRSVVLTSHSMEECEALCTRLGIMVNGRFKCLGSIQHLKNRFGDGYMITVRTKTTASVKEVIRFFNRNFPEAILKERHHTKIQYQLKSENISLAQVFSKMEQVVEVLSIEDYSVSQTTLDNVFVNFAKKQSDNLEQQESSPSSGGQSPLQHLLSLLRPRPANTELNALVSEEPEELESDDDEGLISFEEERVQLSFNTDTLC

Target ORF information:

RefSeq Version	XM_005165231.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATP-binding cassette, sub-family A (ABC1), member 2 (abca2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165231.4

ORF Insert Sequence:

ATGGGATTTC TTCACCAGCT ACACCTTCTC CTGTGGAAGA ACGTGACTTT AAAGAGGAGA 
GGACCGTGGG TTCTTGCATT TGAGATTTTC ATCCCATTGG TGCTCTTCTT CATACTCCTG 
GGCTTGCGGC AGAAGAAGCC AGCCATTCCA GTCAAAGAAG TGTCCTTCTA TAGCGCGGCG 
CCGCTCACCT CGGCGGGCAT CATTCCCATC ATGCAGTCGT TGTGTCCGGA TGGTCAGCGG 
GATGAGTTTG GCTTCCTGCA GTATAAAAAC TCCACTGTGA CTCAATTGCT GGAAAGGATA 
AGTGAAGTTG TGGAGCAGAA TCACTTGTTT ACATCAGACA GGCCCAGTCT GGGAGAGGAG 
CTGGAGTCTC TGCAGCAGCA CCTCGAAAGT CTGAGCTCAG CACAAGGCCC TCTAGAGAGC 
CACTACAACA CCAGCCACGG TTTCACTGTG GGAAGCGTCC TCAGGAACCA GTCGATGTTC 
CAGCAGTTTC TGATGCGAAA TCTCTCCCTG CCGAACGACA CGTCCAGCCT GCTGCTCTCC 
TCCCCCGTCA ATCTAAAAGA GGTATATAAT CTCATCTTTG GGACGTATCC CAAAGCGGGA 
TCAAAGGGAG GAGAAAGAGA TGAAGGACAG CACCAACTCA ACAGTCCTGG AGAGAAGCTT 
GTACAGCTGG AGCAACTACT TGGAGGCTGG CACAGTGTAG ACGGGGGTCT GATTCAGAAG 
GCACTGAGAG ATCCCACAAA AGCTGCTCAC CGTCAAGCTC TTCTCAAAGT CTTCTCTCAG 
GCGTTGGGCA TCGCTGGCGG TGTAGGAGGT CAAAAATTCG ATCCTCAAGC ACTGCGTGAG 
ATGGAGGGAG TTCTGTTGAC TGGTGCAGTG CTTGAAAGCC TTACCTGTGG GGAAGGAGGG 
AACAGTGAGA TTGGCAAAAT CCTATTGGTC CCTGAGAAAC AACAGGCGGT TTTACAGGCT 
TACCGCACGA CTGTTTGTGG TTTAGGGGCG GGGCAGAGGG CGGAGCTTTT CAGACAGTTG 
AGTGAAGAAC TGAGAGATCA GATTGACACA CAGAGAGTCT TTGATCAGTT GCGTCTTGAG 
CAGTCTAATA TGTCTGGTCT GCTGGATCAG TCCCGTCTCG GGGCTCTCCT GAAGAACCTG 
GCTGATGTGG AGCGGCTGCT GAGGGACGTG GATCTACTGT CAGGTCTGGC CAGACTGCTG 
CCGAAAGGGG CCTGTGCGGG TCACCAGCCT CCTCCTCAGG CCAACACCAC CTCCTGGAGC 
TCCAACTCTA CCACATGGGG TCCCAATACA ACTGACAGTT CTGTGGAGGA AGGAGAAGGA 
GCTTCTGAGA GCGAGGCAGA GGCTGAAAAC CCCAAATCAC AGTTTTCTGC ATTTGTTCAG 
CTGTGGGCAG GACTTCAACC CATACTTTGT GGGAATAATA GGATTTTCGA ACCAGAAGCG 
CTGAAACAAG GAAATATGAG TTCTCTGGGC TTCACCAGCA AAGAACAGAG AAATTTAGGA 
TTACTTGTTC ATCTCATGAC TACAAATCCC AAAATCCTCT ACTCTCCCAT TGACTCAGAG 
GTGGACAAGG TCATACAAAA GGCCAATGAG ACCTTTGCTT TTGTGGGGAA TGTGACTCAC 
TATGCCCGTG TGTGGCTCAA CATCTCTGCT GAATTAAGGG CCTTTTTAGA GGAGGGTAAA 
TTGCAAAATC ACCTGATGTG GCTCCAGCAG TTTATTACCG ACCTGAAGAG GCACCATGAG 
CTGCTGAACG TATCAGACAG TGAGCTGATG AACAGTCTGC TGGAGGAGAA CTTCACCCTG 
CCAAACACAA CCACCTTACT GGAGCAGCTG CACACCATTG ATAATGCTGC TTGTGGATGG 
ACTAACTTCA TGTCAAAGGT CAGTGTGGAT ATCTTCAGAG GCTTCCCTGA TGAAGAAAGC 
ATTGTCAATT ACACACTGAA CCAGGCCTAC CACGACAACG TCTCAGTCTT TGCAAGTGTG 
ATTTTCCAGA CCAATAAAGA TGGATCCCTG CCACCCCATG TTATGTATAA AATTCGACAG 
AACTCGAGCT TTACCGAGAA AACCAATGAA ATCCGACGTG CTTATTGGCG CCCAGGGCCA 
AACACAGGCG GCAAATTCTA CTTCCTTTAT GGCTTTGTGT GGATCCAAGA TATGATTGAA 
CGGGCAATCA TCAACACCTT TGTTGGCCAC GATGTTGTGG AGCCTGGAAA TTACGTGCAG 
ATGTTTCCTT ATCCGTGCTA CACCAGAGAC GATTTTCTCT TTGTTATCGA GCACATGATG 
CCTCTGTGTA TGGTCATATC CTGGGTCTAC TCTGTGGCCA TGATGATCCA GCACATCGTA 
GCAGAGAAGG AGCAAAGACT GAAAGAGGTA ATGAAGATGA TGGGCTTGAA TAACGCAGTG 
CACTGGGTGG CCTGGTTCAT CACAGGCTTC GTGCAGCTTT CCATCTCAGT GACGGCCCTC 
ACTGCCATAC TGAAATACGG GAAAGTCCTC CTTCACAGCG ATCCCTTCAT CATATGGCTT 
TTTCTTACAA TCTACGCTGT CGCAACCATC ATGTTCTGTT TCCTGGTGTC GGTGTTGTAC 
TCTAAAGCCA AACTGGCCTC GGCATGTGGA GGAATCATCT ACTTCCTGAG TTATGTGCCC 
TACATGTATG TGGCCATAAG AGAAGAGGTG GCTCACGATA AGATCACTGC CTTTGAGAAG 
TGCATAGCGT CTTTGATGTC TACCACAGCG TTCGGTCTGG GATCCAAGTA CTTTGCCCTG 
TATGAAGTGG CTGGCGTGGG AATTCAGTGG CGCACTATTA ACCAGTCACC TGTAGAGGGC 
GATGACTTCA ATCTACTGCT GTCTATGGTC ATGCTCACTA TTGACGCCAT TGTATATGGT 
GTGTTGACCT GGTATATAGA AGCCGTCCAT CCAGGGATGT ATGGACTGCC TCGGCCATGG 
TATTTCCCCT TGCAGAAGTC CTATTGGTTA GGTAGTGGCC GCATAGAGAC GTGGGAGTGG 
CCGTGGGGTG GAGGCACCAG GCTGAGTGTT ATGGAAGAGG ACCAGGCTTG TGCCATGGAG 
CATAGACGCT CAGAGGAAAC CCGTGGAATT GAAGAAGAGC CCAATCATTT GCCGCTGGTG 
GTGTGCATCG ACAAACTGAC CAAAGTCTAC AAGACGGGCA GCAAACTGGC TCTAAACAAG 
CTGAGCCTGA ACCTGCATGA GAACCAGGTT GTCTCTTTCT TGGGACACAA TGGGGCTGGC 
AAGACCACCA CTATGTCCAT TCTCACGGGA CTCTTTCCAC CCACCTCTGG CTCTGCCACC 
ATATACGGCC ATGACATCCG CACAGAGATG GAGCGCATCA GGCAGAACTT GGGCATGTGT 
CCCCAGCACA ACGTCCTGTT TGATAAGCTC AGTGTGGAGG AACATCTGTG GTTCTACTCA 
CGGCTGAAAG GCATGGCAGA GGAAGATATT CGCAAGGAGA TGGACAAGAT GATAGAGGAT 
CTCGAATTAT CTAATAAACG TCACAGTCTG GTTCAAACCC TGTCAGGAGG GATGAAAAGG 
AAGCTGTCAG TGGCCATTGC TTTTGTAGGT GGGTCTCGGG CAGTGATTTT GGATGAACCA 
ACAGCAGGAG TAGACCCATA TGCCCGTCGA GCGATCTGGG ACCTCATCCT GAAATATAAG 
CAAGGTCGCA CCATCCTGCT GTCCACTCAC CACATGGATG AGGCTGATCT GCTGGGTGAC 
CGCATTGCCA TCATCTCTCA TGGCAAGCTC AAGTGCTGCG GTTCTCCTCT GTTCCTCAAG 
AGCACATATG GAGACGGATA CAAACTCACA CTTGTTAAAA AACAGAGTGA CTCACACACT 
GCAGATCAGT CAAGCCAGTC TCCGTCCTCT TCGATATCTC CATGTTCAGA AAACAGAGTA 
ACTCAGTTCA TCCGTCAGTA TGTTGCTTCC TGCCTGCTGG TGTCCGACTC CAACACTGAA 
CTCTCTTATG TCCTACCATC AGAGGCTGTC AAGAAAGGCT GCTTTGAGAG ACTCTTCCAG 
GCTCTGGAGC AGAGTTTAGA CAGCTTGGCC TTGACCAGCT TTGGCGTGAT GGACACCACA 
TTGGAGGAGG TGTTTCTAAA GGTGTCAGAA GAAGATCTGT CGCTGGAAAA CAGCGATGCA 
GACATGAAGG ACTCTCCAGG CGGAGCATCA GCCGGGAAGC CGTCAGGTCT GTTAGGAGGG 
CCTCAGTGTG AAGGAGCTCC AGCCGCTGCT GTGATGAGGC CAGAGGTGGA GCTCAGCAAC 
CTGATGATGT GCTCCAGGCT GAGCCCAAGC CAAGCATCCC TGCAGTCCGG CTCTTCTCTG 
GGCTCAGTGA GAGGAGATGA AGGGGGTCCC TATTCTGAGT TCTATGGAGA CTATTGCCCA 
CTGTTTGACA ACGGTCAGGA TCTGGACTCT GCCAGTCTCA GAGATGAAGA GTCATCTCCT 
CAGCCCACTG TACTCGAGGG CCAGGGCAGC TTCAAGCTCG ATGGCTGGTG GCTTAAACTC 
CGCCAGTTTC ATGGGCTGAT CGTGAAAAGG TTTCACTGTG CCAAGAGGAA CACCAAGGGC 
ATTTTCTCTC AGATTCTGCT GCCTGCTTTC TTCGTATGTG TGGCAATGAC TGTGGCTCTG 
TCTGTGCCTG AGATTGGTGA CCTTCCTCCC CTGATCCTGT CTCCATCTCA ATATCACAAC 
TACACACAGC CTCGAGGAAA CTTCATCCCC TACGCCAATG AAGACAGATC TCAATACAGG 
ACTAAGTTTT CTCCAGACGC AGGCCCACAG AGGATTGTTA ACACCCTCCG TCTGCCATCT 
GGAGTCGGTG CGACTTGTAT CCTGAAGACT CCCTTTAACA GCACCCTGGA CCAACTGGCC 
CAAACTCTCA ATCCTTATGC CAACAACTCT AAAACCCTGG CAGCCCGCTA CTTTGACTCT 
ATGTGCCTGG ACTCCTTCAC GCAGGGTGTG CCTTTGTCGA ATTTCGTTCC CCCTCCGCCC 
TCTCCTGCCC CCTCAGACGA CCCGGAGGCT CATTTTGAAG ACGGCCTGTG GAACTACACT 
GCAGCTCCTA CTACTGTCAG AGAGATGGCC ACGTCTCCCC CGACTCTGCC CTTGAGTATC 
CATGAGCCGG TGCGCTGTAT CTGCTCTATG CAGGGCACAG GCTTCTCCTG CCCCAGTGGA 
GTTGGGGGTC GACCTCCTCT GATGAAAGTG GTGACCGGAG ACATCCTGGT GGACATTACA 
GGTCGCAACG TGTCTGAATA TCTCCTGTAC ACGTCTGATC GGCTCAGGTT ACACAGATAT 
GGAGGCTTTA CTGTTGGAAA CATTCAGAAA TCTGTCCCAG CATCCTTCGG GAGAAAAACA 
CCACCTATGG TGCGCAAAAT AGCAGTGAGG CGATCCTCAC AGGTGCTGTA TAATAACAAA 
GGCTATCACA GTATGCCAAC ATATCTAAAT GTCCTCAACA ATGCCATTCT CAGAGCCAAT 
CTGCCCAGTA GTAAAGGAAA CCCTTCTGCA TACGGCATTA CAGTGACCAA TCATCCCATG 
AACAGAACCA GTGCCAGTCT TTCTCTGGAT TATCTGCTCC AGGGCACTGA TGTGGTTATT 
GCCATCTTCA TCATTGTAGC CATGTCTTTC GTGCCTGCGA GCTTTGTGGT CTTTCTGGTA 
GCGGAAAAAT CCACCAAAGC CAAACACCTG CAGTTTGTCA GCGGATGCGA CCCTGTCACG 
TACTGGCTAG CAAACTACAT ATGGGACATG CTGAACTACC TGGTTCCAGC TACATGCTGT 
GTCCTAATTT TGTTTGTGTT CGACCTTCCT GCGTACACGT CACCTACAAA TTTCCCAGCA 
GTGCTCTCTC TGTTTCTGCT TTATGGCTGG TCTATAACCC CCATCATGTA CCCAGCCTCC 
TTCTGGTTTG AGGTGCCCAG CACAGCTTAT GTTTTCCTCA TAGTCATCAA TCTCTTCATT 
GGCATCACAG CCACGGTGGC CACTTTCCTC CTCCAGCTGT TTGAGCGCGA CAAGGACCTG 
AAGTTGGTAA ACAGCTACCT GAAGTCATGT TTTCTCATCT TCCCCAACTA TAACCTGGGT 
CACGGATTGA TGGAGATGGC CTATAATGAG TATATCAATG AATATTACGC CAAGATAGGC 
CAGTTTGATA AAGTGAAGTC TCCATTTGAA TGGGACATTG TGACGCGGGG ACTGGTAGCA 
ATGACCATTG AGGGCTTCGT CGGCTTTCTC ATTACAATTC TGTGCCAGTA CAACTTCCTC 
AGGAAAGCCC AGAGAGTGCC TGTCAACAGT CAGCCAGTGG AGGACGATGA TGTTGATGTG 
GCCTGTGAAA GACGCCGAGT TCTGAGGGGA GACGCTGACA ACGACATGCT GAAGATCGAC 
AACCTAACCA AGGTGTACAA GTCTCGTAAG ATGGGCCGGA TCCTGGCAGT GGACCGATTG 
TGTCTGGGGG TCCGTCCTGG TGAATGCTTC GGTCTGCTGG GAGTGAATGG GGCAGGAAAG 
ACCACCACCT TCAAAATGTT GACAGGAGAT GAGAGCACAA CAGGGGGAGA AGCCTTTATC 
CAGGAACACA GTATCTTGCG GGAGCTGTTA CGTGTGCAGC AGAGCATCGG TTACTGCCCT 
CAGTTTGACG CTCTGTTTGA TGATCTGACG GCTCGAGAGC ATCTGGAGCT CTACACGCGC 
CTCCGCGGCA TCCCATGGAA AGATGAGGAG CGGGTGGTTC AGTGGGCTTT GGAGAAGTTG 
GAGTTGTCCA AGTATGCCGA CAAGCCTGCT GGCACCTACA GCGGAGGCAA CAAACGCAAA 
CTCTCTACAG CCATTGCTCT GATTGGATAC CCATCGCTCA TCTTTTTGGA TGAGCCCACC 
ACAGGTATGG ACCCAAAGGC TCGCCGTTTC TTATGGAACC TAATTCTTGA CATCATCAAG 
ACGGGCCGCT CAGTAGTGTT GACGTCACAC AGTATGGAGG AGTGTGAAGC GCTGTGCACT 
AGACTGGGCA TCATGGTTAA TGGCAGGTTC AAGTGTCTAG GAAGCATCCA GCATCTCAAA 
AACAGATTCG GAGATGGATA CATGATAACA GTACGCACTA AGACCACCGC TAGCGTTAAA 
GAAGTGATTC GGTTTTTTAA CAGAAATTTT CCTGAGGCAA TTCTAAAGGA GCGACACCAC 
ACAAAGATTC AGTACCAGCT GAAGTCAGAG AACATCTCTC TGGCTCAGGT CTTCAGCAAA 
ATGGAGCAGG TGGTGGAGGT GCTGAGCATC GAGGATTATT CCGTCAGCCA AACCACACTG 
GATAACGTCT TTGTTAACTT TGCAAAAAAG CAAAGCGACA ACTTAGAGCA GCAAGAAAGT 
TCTCCATCTA GTGGTGGCCA GTCGCCACTG CAGCATCTGC TCAGCCTCCT CAGGCCTCGT 
CCTGCAAACA CTGAGCTCAA CGCCCTCGTG AGTGAAGAGC CTGAGGAACT GGAGAGTGAT 
GATGACGAAG GACTGATCAG CTTTGAGGAA GAGAGGGTGC AGTTGTCCTT TAATACAGAC 
ACTCTGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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abca2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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