FN1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	FN1A
Entrez Gene ID	100005469
Full Name	fibronectin 1a
Synonyms	Fn,fb80d10,fn1,fn2,wu:fb80d10
General protein information	Preferred Names fibronectin 1a Names fibronectin fibronectin 1 fn1l nat natter
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131520.2	NP_571595.2	fibronectin precursor
XM_009304503.3	XP_009302778.1	fibronectin isoform X1
XM_009304503.2	XP_009302778.1	fibronectin isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FN1	XP_516072.3
Macaca mulatta (Rhesus monkey)	FN1	XP_001083548.2
Mus musculus (house mouse)	Fn1	NP_034363.1
Gallus gallus (chicken)	FN1	NP_001185641.1
Danio rerio (zebrafish)	fn1a	NP_571595.1
Homo sapiens (human)	FN1	NP_997647.1
Canis lupus familiaris (dog)	FN1	XP_005640798.1
Rattus norvegicus (Norway rat)	Fn1	NP_062016.2
Xenopus tropicalis (tropical clawed frog)	fn1	NP_001025695.1

Gene Ontology
Bibliography

Related articles in PubMed

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Xue Y, Liu D, Cui G, Ding Y, Ai D, Gao S, Zhang Y, Suo S, Wang X, Lv P, Zhou C, Li Y, Chen X, Peng G, Jing N, Han JJ, Liu F
Cell reports27(5)1567-1578.e5(2019 04)

The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling.
Rasouli SJ, El-Brolosy M, Tsedeke AT, Bensimon-Brito A, Ghanbari P, Maischein HM, Kuenne C, Stainier DY
eLife7(2018 12)

Vangl2-dependent regulation of membrane protrusions and directed migration requires a fibronectin extracellular matrix.
Love AM, Prince DJ, Jessen JR
Development (Cambridge, England)145(22)(2018 11)

Glypican 4 and Mmp14 interact in regulating the migration of anterior endodermal cells by limiting extracellular matrix deposition.
Hu B, Gao Y, Davies L, Woo S, Topczewski J, Jessen JR, Lin F
Development (Cambridge, England)145(17)(2018 08)

Transient fibrosis resolves via fibroblast inactivation in the regenerating zebrafish heart.
Sánchez-Iranzo H, Galardi-Castilla M, Sanz-Morejón A, González-Rosa JM, Costa R, Ernst A, Sainz de Aja J, Langa X, Mercader N
Proceedings of the National Academy of Sciences of the United States of America115(16)4188-4193(2018 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FN1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FN1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa98517	NM_131520.2 Latest version!	Danio rerio fibronectin 1a (fn1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa48047	XM_009304503.3 Latest version!	Danio rerio fibronectin 1a (fn1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa48047	XM_009304503.2	Danio rerio fibronectin 1a (fn1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FN1A ( NM_131520.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571595.2 Danio rerio fibronectin 1a (fn1a), mRNA.

Price & Availability↑

CloneID	ODa98517
Clone ID Related Accession (Same CDS sequence)	NM_131520.2
Accession Version	NM_131520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570204800000
Organism	Danio rerio(zebrafish)
Product	fibronectin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX323049.20. On Oct 17, 2015 this sequence version replaced NM_131520.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF081128.1, BC162574.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGGTG GCCCTTTGGG CACGGCGCTT GCGGTGGCAT TGCTTGCCTG CGCTGTGCAG 
TGTATGCCGA AAGGAACGGG GAAACATAAA AGACAAGCTC AGGAACACTC TGTATCATCT 
GTGTCACAAG ATGGATGTGT TATGGACGGA CAGTTTTATG GAGCTGGAGA GAAGTGGGAG 
CGCATTTATC TGGGCAGCAC GCTTCTTTGC ACTTGTCATG GAGTTTCTAA AATAGAGTGT 
AAATCAAAAC CTGATGCTGA GGAGACCTGC TACGATAAAG TCAATTCTCG TTCCTACCGG 
GTTGGAGAGA CTTACGAGAG GCCCAAGGAC GGAATGATCT GGGACTGTAC TTGCATTGGC 
TCTGCCCGAG GGAAAATCAG TTGCACCATT GCGAACCGCT GTCATGAGGG AGGGCACTCC 
TACAAGATTG GAGACACATG GCAGAGACCT CATGACACTG GAGACTACAT GCTGGAGTGT 
GTCTGCTTGG GAAATGGGAA GGGGGAGTGG ACCTGTAAAC CTGTTGCGGA GCGCTGTTAT 
GATAATACAG CTGGCACCTC CTACATGGTT GGTGAGACCT GGGAGAAGCC CTACCAGGGC 
TGGATGATTG TGGACTGCAC CTGCCTCGGA GAAGGAAGTG GACGTATTAC ATGCACATCC 
AGAAACCGCT GTAATGACCA GGACACACGC ACTTCTTACC GGATTGGGGA CACGTGGAGT 
AAGACGGACT CTCGTGGACA CGTTTTACAG TGTTTGTGTA CTGGTAATGG ACGTGGAGAG 
TGGAAGTGTG AGAGGCATGC TTCTCTGCAC ACTACTAGTC TTGGTACTGG GTCTCGTGTT 
GTGACCAATG TCCAGCCAGC AGTGTACCAC CCACAAGGGG TGCCTGAACA CCCTGTGGAG 
GGATCCTGTC TGACTGAAGC AGGTGTTTCC TATGCTCCAG GCATGCGCTG GAGCAAGGCT 
CAAGGTAGCA AGCAGATGTT GTGTACCTGC TTAGGAAACG GTGTCAGCTG TGAAGAGTCA 
GAGAGTCAAT CTCAGGTGTA TGGCGGGACC TCAGGTGGCG AACCGTGTGC TTTCCCTTTT 
GTGTTCATGG GGAAAACGTT TTACTCCTGC ACGTCTGAGG GACGCAATGA TGGACAGCTT 
TGGTGCAGCA CCTCATCTGA CTTTGAGAAA GACTACAAGT ATTCCTTCTG TACCAGCAAC 
AATGTTGTGG TCACAACCAG AGGAGGAAAC TCTAATGGTG CCCTGTGCCA GTTCCCTTTC 
CTCTACAATG GCCGTAACTA CACTGACTGC ACTGCTGATG GCAGAAGAGA TGGCATGAAA 
TGGTGCGGCA CGACTTATAA CTTTGACAAA GAGCAGCGCT TTGGCTTCTG TCCAATGGCA 
GCTCATGAAG AGGTGTGTAC CACCAATGAG GGTGTGATGT ATCGTGTGGG GGATCAATGG 
GACAGACGCC ATGATGTTCT GGGTCACATG ATGCGCTGTA CGTGTGTGGG CAATGGCCGT 
GGAGAATGGA GCTGCATCGC CTACTCTCAA CTCAAAGACC AGTGCATAGT GGATAATTTG 
ACCTATGAAG TAAATCAGAC TTTTACTAAA AGGCACGACG AAGGCTACAC AATGAACTGC 
ACCTGTTTCG GTCAAGGTCG TGGACGCTGG AAGTGTGATG CCATCGATCA GTGCCAGGAG 
CCGGAGACTA GAGTTTTCTA TCAGATTGGA GAGTCCTGGG ACAAACTCAT TCAGGGAATC 
CACTACAGAT GTTACTGCTA TGGTAATGGC ATCGGGGAGT TGAGCTGCGA GCCTCAGCAT 
TCTATCTCTG GTGGTCATCG TCCAGTCCAG GTCATTATTT CAGAAGCTGG AAATCAGCCT 
AACTCGCACC CCATCCAATG GAATGCACCA GCTTCTGCTC ACATCACACA GTACATACTT 
AAATGGAGGC CGAAAAACAC CCATATTCAG TGGATGGAGG TGACTATTCC AGGCCATGTC 
AACTCTTACA CTATTGCGGG ACTGAAGCCG GGAGTCACCT ACGAGGGTCA GCTCATCAGT 
ATCCTGCGTT TTGGACGTAG AGAGACCACT CGCTTTGATT TTTCCACCGT ACATGGCTCA 
TTGGAGACCT CTGAGGGTGA GACCACCCAG CCCCCTCGAA TGGTGGACAC CTCTGAGTCA 
GTGACTGAAA TCACCTCAAG CAGTTTCGTC ATCTCCTGGG TGTCTGCGTC TGACACCGTG 
TCTGGTTTTA GGGTGGAATA TGAGCTGAGT GAAGAGGGAG GCCAGACTGG ACAGCCCATG 
ATCTTGGACC TCCCTCATAG CGCTACGTCC GTGAACATCA GTGAACTTCT GCCAGGAAGG 
AAGTACACTG TGAATGTATA TGAAGTGACA GGAGAAGGAG AACCTAACCT CATCCTCACC 
ACTTCCCAGA CTACTGCTCC TGATGCTCCT TCTGATCATG AAGTGGAGGA TGTAGCAGAC 
ACCTCCATTC GTATCAGCTG GTCTAAACCT TTGGCTCCCA TCACAGGTTA CCGTGTAGTT 
TACACCCCAT CTGAAGAGGG CAGTTCTGGC AGTGTTGAGC TGAATCTGCC AGAACATTCC 
ACCTCATTGA CCCTTGGTGA TCTTCGACCT GGTGTGCTTT ATAACATCAG CATCTTCTCT 
GTGGAAGAGA ATCAGGAGAG TGAACCTGTT TTTGTTCAGG TCACCACTGC TGGAGAGCCA 
CTGGCGGAGG AAGTGCCGTC TCCAACCGAT CTGCAGTTTT ATGAGGTGTC CGACTCAAAG 
ATCACTATCA CCTGGACTTC TCCGTCCTCT GAGGTGTCTG GATACCGTGT GTCTGTGGGT 
GAAGTGGGTC CAGATGGCTT GATTGAGAAA GAAGTGCCTT ATCCAGTAAC ACAGAATGCT 
TATGCTGAAA TCACACACCT TCAGCCCGGC ACCCTCTACC GCTTCTTCAT CTTTTCCCTC 
AAATCAGGAG AGGAGAGCGA ACCGCTGGTG GGAGAGCAAG CTACAAAGCC GGATCCCCCA 
ACTGACATCT ACTTCTCCAA CATTACTGAA GACAGTGCTG TGATCGTCTG GCTGCCTCCC 
ACTGCTCAGA TCACAGGTTA CAGGCTTTTC CTGACAGTCG AGGGCTCCAA CCCCAAACAG 
CTGCGTATTC CAGGCCGTCT GTCGCAGTAC ACCCTCCTCA ACCTGCAGCC GGACACAGAA 
TACTCTGCCA CTCTGCATGC AGAAAGAGGA AACGTGCTCA GTGAAGGCTC CATTGCTACC 
TTCGCCACAA CTCCTCCAAT GGGAAATGCT CCGTACTTTA GCACTGATGT CACAGACACT 
TCTATCATTG TCTCCTGGAG CCCTCTGCCC AAAATTGGAT ACAAGTTGAC AGTGAGGCCC 
AGCCAAGGTG GAGAAGCCCC TAGAGATGTG ACCTCAGAGT TGGGTAGTGT CCTCATCTCA 
GGTCTGACCC CTGGAGTTGA GTACACATAC AGTGTCCAAC CAGTCATCAG TGGTCAAGAG 
CATGGAAACC CTATAACTCG GCGCGTGGTC ACTCCTCTTT CTCCACCCAC TGATCTGAAC 
CTGGAGTCCA ACCCCAACAC CGGAAAGCTA ACTGTTCAGT GGAATGATGC TAACATCCCA 
GACATCACGG GCTACAGAGT GACCTGCACC CCCACCAAAG GCCAGCAGGG CAATTCACTT 
GAGGAGTTTG TGAAGGCCGG ACAGAACTCA TGCACATTAG AGAACCTGAG TCCAGGAGTA 
GAGTACAATG TCAGCGTGTT CACTGTGAAG GACGACATGG AGAGCGTTCC TGTCTTCACA 
ACTGTAACCC CAGATGTGCC TAAAATAACT GATCTCAGCT TCATCAATGT CACTGACTCT 
ACTATTGGCC TAAGCTGGAG TCCTCTAAAC TCCACTGCCG TTACCGGCTA CCGCATCACA 
GTTTTGGCAG CTGGGGACAG TGTCCCGATC TTTGTTGAAT TTGTGGAGCC CACAACAGGC 
TTTTATACTG TTCATGGCCT GGAGCCAGGC ATAGATTATG ACATCACCGT CACCACAGTT 
ACCGAAAATG GAGAGAGCGA ACCCATCACT ATCACCCAGC AAACAGCTGT CCCGGCTCCA 
TATGGCCTGT CATTTGGAGA AGTGACCGCA GACACTATGT TGGTGACCTG GAAAGCCCCT 
CAGGTTCCCA AATCCTCAGA CATTAATCAA TACATCATCC GTTATCATCC AGTCGATGAA 
GATGACGAAA CTACTGAACG CACTGTGGAA GGAAGTGAAA ACTTTGTTGT CCTTCGCCAT 
CTTGTGCCCA ACACAGAGTA CCTGGTGAGT GTGATTTGCG TATATGAGGG CAGAGAGGGA 
TCACCTGCCA TCGGCACCCA GAGAACTGTT TTTGATGCCC CTGTTGGCCT TCAGTTCTCT 
GATGTGGGCA CCACCTCCTT CACTGTGCGC TGGCAGGCTC CTCGGGCCAT CATCTCAGGA 
TACCGTATCC GCTACCAGAT GACCAGTGGT GGACGAGCTA AAGAGGAGAG ACTGCCCCCC 
TCGAGGAGCC ACTTCACCCT GACAGGCCTG ACCCCAGAGA CTGAGTACAG CATCAGTGTC 
TATGCCGTCA GCGGATCCAG AGACAGTCTG CCTCTGACTG GAACTCAGTC TACCATCTCT 
GATGCTCCTA CTGACCTGGA AGTGATTTCC TCAACGCCGA CCAGCATCAC CGTTCGCTGG 
GATGCCCCCT CTGTCACTGT GCGGTACTAC AGGATTACAC ATGGAGAGTC GGGTGGTTCT 
GATGCCCCTC TGGAATTTAT GGTTCCCGGC TCCCAGTCTA CAGCCACTAT TGAAGATCTA 
CGTCCAGGCA CTGATTATAC CATTACTGTT TATGCTGTGA CCGGTAGAGG GGACAGCCCT 
GCATCCAGCA CTCCCATCCA TGTTAAACAT AGAACCGATA TTGACTCTCC ATCACAGATG 
GAGGTGACTG ACGTGAAGGA CAACACTATT ACTGTACGCT GGACCCCAGC AGCCGGCCCC 
ATCTCAAGAT ACAGAGTCAA TGCAACTCCT CTTACTGGAG AAGGACCTGT GTTACATGCA 
GAAACGACTT CAGACCATAC TGAAATTACG TTTTCTGGCC TGATGCCTGC AGTGGAGTAC 
AGCATCAAGG TGTATGCTCT GGGTCAGGAT GGAGAGAGCC TCCCTCTGGT CGAAAATGTA 
GTTACAACGG TGGACAAGCC CAAGGATCTG TCCTTCACTG ACGTGGAATC CAGCTCTATG 
CGCATCTCCT GGGACAGTCC CGATGGCGTG GTCTCCTCCT ACAGAGTGTT GTACTACAGT 
CCAGAGGAGG GAGAGAGAGA GCTGTTTCCT GCCCCGCATG GAGATGACGA GTCTGCAGTA 
CTTCATGGCC TCAGACCCGG CACAGAATAT ACTGTTAAAG TCATCGCACT GCATGACCAA 
TCACCCAGCA CTGCACTGGT CGGCACTCAG ACAACAGGCA TTCCTGGACC CACAAGACTG 
CAGTTTTCCC AGGTGGGCCC AACATCCTTC ACAGTCTCCT GGTCTTCATC TGACGCCAGT 
TTGACAGGTT ATCGTGTGGC TGTTTCACCC AAGAGCAAAA GTGGCCCTAC TAAAGAAGAA 
AACATCACTC CTGACTCCAC TGAGTTCCAC GCCACTGGAC TCATGCCTGG CACTGATTAC 
GAAGTGGAGG TTTATGGTGT GAAGAACTCT CTAACCAGCC CTCGTGTCAA AGGAGAAATC 
ACAACACCTG ACAATATCAG TCCACCTCGA CGTGTCCGTA TATCCAATGT GAAAGACTCC 
TCCATCACTC TGACCTGGCG CTCAAAGACT GAGGCCATTT CTGGCTTCTT GGTGGAAGCA 
ACTCCCACCA TGGGTGGCCA TAATCCAATC CAGAGGACCA TTGAACCTGA TTCACGCACC 
TACACAATTG CTGGTCTGGA GCCAGGAACT AATTATAAGA TCAATATTTA TACTCTGAAT 
GGAAACTCTC GTAGTGAGCC TTTCACCCTT ACAGCTACTA CAGCAAAACC AGTTATTAGC 
CCACCTACAA ACCTCCATTT TACTTCTCTG GCTTCTACTT CAATCTCCTT CACCTGGGAG 
CCTCCACGCA GCACTATTAC TGGATATTAT GTGACCTATG AGGAGGCTGG AGGCATTCCC 
AAAGAGCTGA CCCCTCGACC CCAGGCTGCC AGAACCTTCG CGTCCATCTC TGGGTTGATA 
CCAGGCACTG AGTACATTAT CAAGATTGTT GCATTAAATG GCGCTCAGAG AAGCACGCCA 
TTGATTGGAA AGGCAAGAAC TCAACTGGAA ATTGAGCAGC TTCCCCCTCA GCTACCTAAT 
CCCAGCCGAC CCCATCTCGA AGTCTTGGAT GTGCCCGAGG ACAATGACAA CTTCAACAAC 
CACGTACATG TGCTGGGGCC AAACAGACAC AACACACTGG GGCAGCAGGG TCAACACATC 
TACACCGAAT ATCAGAGCTT CAACCTGGGC AACAACGGCC AGCAGCCTCA TCATCCAAAC 
CACGGCGAGC CGCTAGTCTA CATCCCTCTG CCGGGAGCGG ACGGCCAGAG AGTGCCTGTG 
GTGAAGGTCA ACGAGGGTCA AGAAGCAGGT TTCCCCTTTG GCGGCCTTTT TAATGAGACA 
AACCTGCCCC AGGAATCTCA GACTCAGACT ACAATTGTGT GGCAGCCTGT GCCACACACG 
TCTGAGTATG TGGTTTGGTG CGACCCCATT ACTGAGATCA ATGAGAAGAG CTTCCAGATG 
CGTCTGCCTG GTACATCAAC AAGTGCTACC CTGATTGGCC TTACCTCAGG TGCCTCCTAT 
AATGTTCTTG TGGAGTCTGT TAATGGAGAC CAAAAACAGA AAGTGCTGCA AGACGTTGTG 
ACAGTAGGAA ATAGCGTTCC AGGAGCTGGA GTGATTCCCA CAGGCAGGGA TGTGTGTTAT 
GATTCATTCA CCGCCACCTA TCATGAGGTT AACACGGAGT GGGAACGCAT GTCCGAGACA 
GGCTTTAAAC TTTGGTGCAA GTGCCTGGGC CTTGGCAGTG GTCATTTCAG ATGTGATTCA 
TCCAAATGGT GCCATGACAG TGGCCACAAC TACCGAATCG GTGAGAAATG GGACCGGCGT 
GCTGAGAACG GCCACATGAT GAGCTGTACA TGTCTGGGAA ATGGGAAAGG AGAGTTTAAG 
TGTGAACCTC ATGAATCCAC CTGTTATGAC GAGGGGAAAT TGTACCAGGT GGGCAACCAG 
TGGCAAAAGG AGTATCTTGG AGCCATTTGC ACATGTACCT GCTACGGAGG ACAACAGGGC 
TGGCGCTGTG AGAACTGTCG CCGTCCTGGT GCTGAAGTTG ATGCAGACCT CATTCAGCCT 
CCAGTACGCA CTGATGCTTT CGACCGATAC AGGGAGAACG CTCTACGCAA ACTGAACATC 
CAGTGTCCCA TTGAGTGCCT GAGACCAGAC CTGTTAGCAG ATGCCCATAG CCCTCATGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571595.2
CDS	138..7580
Translation	MFGGPLGTALAVALLACAVQCMPKGTGKHKRQAQEHSVSSVSQDGCVMDGQFYGAGEKWERIYLGSTLLCTCHGVSKIECKSKPDAEETCYDKVNSRSYRVGETYERPKDGMIWDCTCIGSARGKISCTIANRCHEGGHSYKIGDTWQRPHDTGDYMLECVCLGNGKGEWTCKPVAERCYDNTAGTSYMVGETWEKPYQGWMIVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKTDSRGHVLQCLCTGNGRGEWKCERHASLHTTSLGTGSRVVTNVQPAVYHPQGVPEHPVEGSCLTEAGVSYAPGMRWSKAQGSKQMLCTCLGNGVSCEESESQSQVYGGTSGGEPCAFPFVFMGKTFYSCTSEGRNDGQLWCSTSSDFEKDYKYSFCTSNNVVVTTRGGNSNGALCQFPFLYNGRNYTDCTADGRRDGMKWCGTTYNFDKEQRFGFCPMAAHEEVCTTNEGVMYRVGDQWDRRHDVLGHMMRCTCVGNGRGEWSCIAYSQLKDQCIVDNLTYEVNQTFTKRHDEGYTMNCTCFGQGRGRWKCDAIDQCQEPETRVFYQIGESWDKLIQGIHYRCYCYGNGIGELSCEPQHSISGGHRPVQVIISEAGNQPNSHPIQWNAPASAHITQYILKWRPKNTHIQWMEVTIPGHVNSYTIAGLKPGVTYEGQLISILRFGRRETTRFDFSTVHGSLETSEGETTQPPRMVDTSESVTEITSSSFVISWVSASDTVSGFRVEYELSEEGGQTGQPMILDLPHSATSVNISELLPGRKYTVNVYEVTGEGEPNLILTTSQTTAPDAPSDHEVEDVADTSIRISWSKPLAPITGYRVVYTPSEEGSSGSVELNLPEHSTSLTLGDLRPGVLYNISIFSVEENQESEPVFVQVTTAGEPLAEEVPSPTDLQFYEVSDSKITITWTSPSSEVSGYRVSVGEVGPDGLIEKEVPYPVTQNAYAEITHLQPGTLYRFFIFSLKSGEESEPLVGEQATKPDPPTDIYFSNITEDSAVIVWLPPTAQITGYRLFLTVEGSNPKQLRIPGRLSQYTLLNLQPDTEYSATLHAERGNVLSEGSIATFATTPPMGNAPYFSTDVTDTSIIVSWSPLPKIGYKLTVRPSQGGEAPRDVTSELGSVLISGLTPGVEYTYSVQPVISGQEHGNPITRRVVTPLSPPTDLNLESNPNTGKLTVQWNDANIPDITGYRVTCTPTKGQQGNSLEEFVKAGQNSCTLENLSPGVEYNVSVFTVKDDMESVPVFTTVTPDVPKITDLSFINVTDSTIGLSWSPLNSTAVTGYRITVLAAGDSVPIFVEFVEPTTGFYTVHGLEPGIDYDITVTTVTENGESEPITITQQTAVPAPYGLSFGEVTADTMLVTWKAPQVPKSSDINQYIIRYHPVDEDDETTERTVEGSENFVVLRHLVPNTEYLVSVICVYEGREGSPAIGTQRTVFDAPVGLQFSDVGTTSFTVRWQAPRAIISGYRIRYQMTSGGRAKEERLPPSRSHFTLTGLTPETEYSISVYAVSGSRDSLPLTGTQSTISDAPTDLEVISSTPTSITVRWDAPSVTVRYYRITHGESGGSDAPLEFMVPGSQSTATIEDLRPGTDYTITVYAVTGRGDSPASSTPIHVKHRTDIDSPSQMEVTDVKDNTITVRWTPAAGPISRYRVNATPLTGEGPVLHAETTSDHTEITFSGLMPAVEYSIKVYALGQDGESLPLVENVVTTVDKPKDLSFTDVESSSMRISWDSPDGVVSSYRVLYYSPEEGERELFPAPHGDDESAVLHGLRPGTEYTVKVIALHDQSPSTALVGTQTTGIPGPTRLQFSQVGPTSFTVSWSSSDASLTGYRVAVSPKSKSGPTKEENITPDSTEFHATGLMPGTDYEVEVYGVKNSLTSPRVKGEITTPDNISPPRRVRISNVKDSSITLTWRSKTEAISGFLVEATPTMGGHNPIQRTIEPDSRTYTIAGLEPGTNYKINIYTLNGNSRSEPFTLTATTAKPVISPPTNLHFTSLASTSISFTWEPPRSTITGYYVTYEEAGGIPKELTPRPQAARTFASISGLIPGTEYIIKIVALNGAQRSTPLIGKARTQLEIEQLPPQLPNPSRPHLEVLDVPEDNDNFNNHVHVLGPNRHNTLGQQGQHIYTEYQSFNLGNNGQQPHHPNHGEPLVYIPLPGADGQRVPVVKVNEGQEAGFPFGGLFNETNLPQESQTQTTIVWQPVPHTSEYVVWCDPITEINEKSFQMRLPGTSTSATLIGLTSGASYNVLVESVNGDQKQKVLQDVVTVGNSVPGAGVIPTGRDVCYDSFTATYHEVNTEWERMSETGFKLWCKCLGLGSGHFRCDSSKWCHDSGHNYRIGEKWDRRAENGHMMSCTCLGNGKGEFKCEPHESTCYDEGKLYQVGNQWQKEYLGAICTCTCYGGQQGWRCENCRRPGAEVDADLIQPPVRTDAFDRYRENALRKLNIQCPIECLRPDLLADAHSPHD

Target ORF information:

RefSeq Version	NM_131520.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fibronectin 1a (fn1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131520.2

ORF Insert Sequence:

ATGTTTGGTG GCCCTTTGGG CACGGCGCTT GCGGTGGCAT TGCTTGCCTG CGCTGTGCAG 
TGTATGCCGA AAGGAACGGG GAAACATAAA AGACAAGCTC AGGAACACTC TGTATCATCT 
GTGTCACAAG ATGGATGTGT TATGGACGGA CAGTTTTATG GAGCTGGAGA GAAGTGGGAG 
CGCATTTATC TGGGCAGCAC GCTTCTTTGC ACTTGTCATG GAGTTTCTAA AATAGAGTGT 
AAATCAAAAC CTGATGCTGA GGAGACCTGC TACGATAAAG TCAATTCTCG TTCCTACCGG 
GTTGGAGAGA CTTACGAGAG GCCCAAGGAC GGAATGATCT GGGACTGTAC TTGCATTGGC 
TCTGCCCGAG GGAAAATCAG TTGCACCATT GCGAACCGCT GTCATGAGGG AGGGCACTCC 
TACAAGATTG GAGACACATG GCAGAGACCT CATGACACTG GAGACTACAT GCTGGAGTGT 
GTCTGCTTGG GAAATGGGAA GGGGGAGTGG ACCTGTAAAC CTGTTGCGGA GCGCTGTTAT 
GATAATACAG CTGGCACCTC CTACATGGTT GGTGAGACCT GGGAGAAGCC CTACCAGGGC 
TGGATGATTG TGGACTGCAC CTGCCTCGGA GAAGGAAGTG GACGTATTAC ATGCACATCC 
AGAAACCGCT GTAATGACCA GGACACACGC ACTTCTTACC GGATTGGGGA CACGTGGAGT 
AAGACGGACT CTCGTGGACA CGTTTTACAG TGTTTGTGTA CTGGTAATGG ACGTGGAGAG 
TGGAAGTGTG AGAGGCATGC TTCTCTGCAC ACTACTAGTC TTGGTACTGG GTCTCGTGTT 
GTGACCAATG TCCAGCCAGC AGTGTACCAC CCACAAGGGG TGCCTGAACA CCCTGTGGAG 
GGATCCTGTC TGACTGAAGC AGGTGTTTCC TATGCTCCAG GCATGCGCTG GAGCAAGGCT 
CAAGGTAGCA AGCAGATGTT GTGTACCTGC TTAGGAAACG GTGTCAGCTG TGAAGAGTCA 
GAGAGTCAAT CTCAGGTGTA TGGCGGGACC TCAGGTGGCG AACCGTGTGC TTTCCCTTTT 
GTGTTCATGG GGAAAACGTT TTACTCCTGC ACGTCTGAGG GACGCAATGA TGGACAGCTT 
TGGTGCAGCA CCTCATCTGA CTTTGAGAAA GACTACAAGT ATTCCTTCTG TACCAGCAAC 
AATGTTGTGG TCACAACCAG AGGAGGAAAC TCTAATGGTG CCCTGTGCCA GTTCCCTTTC 
CTCTACAATG GCCGTAACTA CACTGACTGC ACTGCTGATG GCAGAAGAGA TGGCATGAAA 
TGGTGCGGCA CGACTTATAA CTTTGACAAA GAGCAGCGCT TTGGCTTCTG TCCAATGGCA 
GCTCATGAAG AGGTGTGTAC CACCAATGAG GGTGTGATGT ATCGTGTGGG GGATCAATGG 
GACAGACGCC ATGATGTTCT GGGTCACATG ATGCGCTGTA CGTGTGTGGG CAATGGCCGT 
GGAGAATGGA GCTGCATCGC CTACTCTCAA CTCAAAGACC AGTGCATAGT GGATAATTTG 
ACCTATGAAG TAAATCAGAC TTTTACTAAA AGGCACGACG AAGGCTACAC AATGAACTGC 
ACCTGTTTCG GTCAAGGTCG TGGACGCTGG AAGTGTGATG CCATCGATCA GTGCCAGGAG 
CCGGAGACTA GAGTTTTCTA TCAGATTGGA GAGTCCTGGG ACAAACTCAT TCAGGGAATC 
CACTACAGAT GTTACTGCTA TGGTAATGGC ATCGGGGAGT TGAGCTGCGA GCCTCAGCAT 
TCTATCTCTG GTGGTCATCG TCCAGTCCAG GTCATTATTT CAGAAGCTGG AAATCAGCCT 
AACTCGCACC CCATCCAATG GAATGCACCA GCTTCTGCTC ACATCACACA GTACATACTT 
AAATGGAGGC CGAAAAACAC CCATATTCAG TGGATGGAGG TGACTATTCC AGGCCATGTC 
AACTCTTACA CTATTGCGGG ACTGAAGCCG GGAGTCACCT ACGAGGGTCA GCTCATCAGT 
ATCCTGCGTT TTGGACGTAG AGAGACCACT CGCTTTGATT TTTCCACCGT ACATGGCTCA 
TTGGAGACCT CTGAGGGTGA GACCACCCAG CCCCCTCGAA TGGTGGACAC CTCTGAGTCA 
GTGACTGAAA TCACCTCAAG CAGTTTCGTC ATCTCCTGGG TGTCTGCGTC TGACACCGTG 
TCTGGTTTTA GGGTGGAATA TGAGCTGAGT GAAGAGGGAG GCCAGACTGG ACAGCCCATG 
ATCTTGGACC TCCCTCATAG CGCTACGTCC GTGAACATCA GTGAACTTCT GCCAGGAAGG 
AAGTACACTG TGAATGTATA TGAAGTGACA GGAGAAGGAG AACCTAACCT CATCCTCACC 
ACTTCCCAGA CTACTGCTCC TGATGCTCCT TCTGATCATG AAGTGGAGGA TGTAGCAGAC 
ACCTCCATTC GTATCAGCTG GTCTAAACCT TTGGCTCCCA TCACAGGTTA CCGTGTAGTT 
TACACCCCAT CTGAAGAGGG CAGTTCTGGC AGTGTTGAGC TGAATCTGCC AGAACATTCC 
ACCTCATTGA CCCTTGGTGA TCTTCGACCT GGTGTGCTTT ATAACATCAG CATCTTCTCT 
GTGGAAGAGA ATCAGGAGAG TGAACCTGTT TTTGTTCAGG TCACCACTGC TGGAGAGCCA 
CTGGCGGAGG AAGTGCCGTC TCCAACCGAT CTGCAGTTTT ATGAGGTGTC CGACTCAAAG 
ATCACTATCA CCTGGACTTC TCCGTCCTCT GAGGTGTCTG GATACCGTGT GTCTGTGGGT 
GAAGTGGGTC CAGATGGCTT GATTGAGAAA GAAGTGCCTT ATCCAGTAAC ACAGAATGCT 
TATGCTGAAA TCACACACCT TCAGCCCGGC ACCCTCTACC GCTTCTTCAT CTTTTCCCTC 
AAATCAGGAG AGGAGAGCGA ACCGCTGGTG GGAGAGCAAG CTACAAAGCC GGATCCCCCA 
ACTGACATCT ACTTCTCCAA CATTACTGAA GACAGTGCTG TGATCGTCTG GCTGCCTCCC 
ACTGCTCAGA TCACAGGTTA CAGGCTTTTC CTGACAGTCG AGGGCTCCAA CCCCAAACAG 
CTGCGTATTC CAGGCCGTCT GTCGCAGTAC ACCCTCCTCA ACCTGCAGCC GGACACAGAA 
TACTCTGCCA CTCTGCATGC AGAAAGAGGA AACGTGCTCA GTGAAGGCTC CATTGCTACC 
TTCGCCACAA CTCCTCCAAT GGGAAATGCT CCGTACTTTA GCACTGATGT CACAGACACT 
TCTATCATTG TCTCCTGGAG CCCTCTGCCC AAAATTGGAT ACAAGTTGAC AGTGAGGCCC 
AGCCAAGGTG GAGAAGCCCC TAGAGATGTG ACCTCAGAGT TGGGTAGTGT CCTCATCTCA 
GGTCTGACCC CTGGAGTTGA GTACACATAC AGTGTCCAAC CAGTCATCAG TGGTCAAGAG 
CATGGAAACC CTATAACTCG GCGCGTGGTC ACTCCTCTTT CTCCACCCAC TGATCTGAAC 
CTGGAGTCCA ACCCCAACAC CGGAAAGCTA ACTGTTCAGT GGAATGATGC TAACATCCCA 
GACATCACGG GCTACAGAGT GACCTGCACC CCCACCAAAG GCCAGCAGGG CAATTCACTT 
GAGGAGTTTG TGAAGGCCGG ACAGAACTCA TGCACATTAG AGAACCTGAG TCCAGGAGTA 
GAGTACAATG TCAGCGTGTT CACTGTGAAG GACGACATGG AGAGCGTTCC TGTCTTCACA 
ACTGTAACCC CAGATGTGCC TAAAATAACT GATCTCAGCT TCATCAATGT CACTGACTCT 
ACTATTGGCC TAAGCTGGAG TCCTCTAAAC TCCACTGCCG TTACCGGCTA CCGCATCACA 
GTTTTGGCAG CTGGGGACAG TGTCCCGATC TTTGTTGAAT TTGTGGAGCC CACAACAGGC 
TTTTATACTG TTCATGGCCT GGAGCCAGGC ATAGATTATG ACATCACCGT CACCACAGTT 
ACCGAAAATG GAGAGAGCGA ACCCATCACT ATCACCCAGC AAACAGCTGT CCCGGCTCCA 
TATGGCCTGT CATTTGGAGA AGTGACCGCA GACACTATGT TGGTGACCTG GAAAGCCCCT 
CAGGTTCCCA AATCCTCAGA CATTAATCAA TACATCATCC GTTATCATCC AGTCGATGAA 
GATGACGAAA CTACTGAACG CACTGTGGAA GGAAGTGAAA ACTTTGTTGT CCTTCGCCAT 
CTTGTGCCCA ACACAGAGTA CCTGGTGAGT GTGATTTGCG TATATGAGGG CAGAGAGGGA 
TCACCTGCCA TCGGCACCCA GAGAACTGTT TTTGATGCCC CTGTTGGCCT TCAGTTCTCT 
GATGTGGGCA CCACCTCCTT CACTGTGCGC TGGCAGGCTC CTCGGGCCAT CATCTCAGGA 
TACCGTATCC GCTACCAGAT GACCAGTGGT GGACGAGCTA AAGAGGAGAG ACTGCCCCCC 
TCGAGGAGCC ACTTCACCCT GACAGGCCTG ACCCCAGAGA CTGAGTACAG CATCAGTGTC 
TATGCCGTCA GCGGATCCAG AGACAGTCTG CCTCTGACTG GAACTCAGTC TACCATCTCT 
GATGCTCCTA CTGACCTGGA AGTGATTTCC TCAACGCCGA CCAGCATCAC CGTTCGCTGG 
GATGCCCCCT CTGTCACTGT GCGGTACTAC AGGATTACAC ATGGAGAGTC GGGTGGTTCT 
GATGCCCCTC TGGAATTTAT GGTTCCCGGC TCCCAGTCTA CAGCCACTAT TGAAGATCTA 
CGTCCAGGCA CTGATTATAC CATTACTGTT TATGCTGTGA CCGGTAGAGG GGACAGCCCT 
GCATCCAGCA CTCCCATCCA TGTTAAACAT AGAACCGATA TTGACTCTCC ATCACAGATG 
GAGGTGACTG ACGTGAAGGA CAACACTATT ACTGTACGCT GGACCCCAGC AGCCGGCCCC 
ATCTCAAGAT ACAGAGTCAA TGCAACTCCT CTTACTGGAG AAGGACCTGT GTTACATGCA 
GAAACGACTT CAGACCATAC TGAAATTACG TTTTCTGGCC TGATGCCTGC AGTGGAGTAC 
AGCATCAAGG TGTATGCTCT GGGTCAGGAT GGAGAGAGCC TCCCTCTGGT CGAAAATGTA 
GTTACAACGG TGGACAAGCC CAAGGATCTG TCCTTCACTG ACGTGGAATC CAGCTCTATG 
CGCATCTCCT GGGACAGTCC CGATGGCGTG GTCTCCTCCT ACAGAGTGTT GTACTACAGT 
CCAGAGGAGG GAGAGAGAGA GCTGTTTCCT GCCCCGCATG GAGATGACGA GTCTGCAGTA 
CTTCATGGCC TCAGACCCGG CACAGAATAT ACTGTTAAAG TCATCGCACT GCATGACCAA 
TCACCCAGCA CTGCACTGGT CGGCACTCAG ACAACAGGCA TTCCTGGACC CACAAGACTG 
CAGTTTTCCC AGGTGGGCCC AACATCCTTC ACAGTCTCCT GGTCTTCATC TGACGCCAGT 
TTGACAGGTT ATCGTGTGGC TGTTTCACCC AAGAGCAAAA GTGGCCCTAC TAAAGAAGAA 
AACATCACTC CTGACTCCAC TGAGTTCCAC GCCACTGGAC TCATGCCTGG CACTGATTAC 
GAAGTGGAGG TTTATGGTGT GAAGAACTCT CTAACCAGCC CTCGTGTCAA AGGAGAAATC 
ACAACACCTG ACAATATCAG TCCACCTCGA CGTGTCCGTA TATCCAATGT GAAAGACTCC 
TCCATCACTC TGACCTGGCG CTCAAAGACT GAGGCCATTT CTGGCTTCTT GGTGGAAGCA 
ACTCCCACCA TGGGTGGCCA TAATCCAATC CAGAGGACCA TTGAACCTGA TTCACGCACC 
TACACAATTG CTGGTCTGGA GCCAGGAACT AATTATAAGA TCAATATTTA TACTCTGAAT 
GGAAACTCTC GTAGTGAGCC TTTCACCCTT ACAGCTACTA CAGCAAAACC AGTTATTAGC 
CCACCTACAA ACCTCCATTT TACTTCTCTG GCTTCTACTT CAATCTCCTT CACCTGGGAG 
CCTCCACGCA GCACTATTAC TGGATATTAT GTGACCTATG AGGAGGCTGG AGGCATTCCC 
AAAGAGCTGA CCCCTCGACC CCAGGCTGCC AGAACCTTCG CGTCCATCTC TGGGTTGATA 
CCAGGCACTG AGTACATTAT CAAGATTGTT GCATTAAATG GCGCTCAGAG AAGCACGCCA 
TTGATTGGAA AGGCAAGAAC TCAACTGGAA ATTGAGCAGC TTCCCCCTCA GCTACCTAAT 
CCCAGCCGAC CCCATCTCGA AGTCTTGGAT GTGCCCGAGG ACAATGACAA CTTCAACAAC 
CACGTACATG TGCTGGGGCC AAACAGACAC AACACACTGG GGCAGCAGGG TCAACACATC 
TACACCGAAT ATCAGAGCTT CAACCTGGGC AACAACGGCC AGCAGCCTCA TCATCCAAAC 
CACGGCGAGC CGCTAGTCTA CATCCCTCTG CCGGGAGCGG ACGGCCAGAG AGTGCCTGTG 
GTGAAGGTCA ACGAGGGTCA AGAAGCAGGT TTCCCCTTTG GCGGCCTTTT TAATGAGACA 
AACCTGCCCC AGGAATCTCA GACTCAGACT ACAATTGTGT GGCAGCCTGT GCCACACACG 
TCTGAGTATG TGGTTTGGTG CGACCCCATT ACTGAGATCA ATGAGAAGAG CTTCCAGATG 
CGTCTGCCTG GTACATCAAC AAGTGCTACC CTGATTGGCC TTACCTCAGG TGCCTCCTAT 
AATGTTCTTG TGGAGTCTGT TAATGGAGAC CAAAAACAGA AAGTGCTGCA AGACGTTGTG 
ACAGTAGGAA ATAGCGTTCC AGGAGCTGGA GTGATTCCCA CAGGCAGGGA TGTGTGTTAT 
GATTCATTCA CCGCCACCTA TCATGAGGTT AACACGGAGT GGGAACGCAT GTCCGAGACA 
GGCTTTAAAC TTTGGTGCAA GTGCCTGGGC CTTGGCAGTG GTCATTTCAG ATGTGATTCA 
TCCAAATGGT GCCATGACAG TGGCCACAAC TACCGAATCG GTGAGAAATG GGACCGGCGT 
GCTGAGAACG GCCACATGAT GAGCTGTACA TGTCTGGGAA ATGGGAAAGG AGAGTTTAAG 
TGTGAACCTC ATGAATCCAC CTGTTATGAC GAGGGGAAAT TGTACCAGGT GGGCAACCAG 
TGGCAAAAGG AGTATCTTGG AGCCATTTGC ACATGTACCT GCTACGGAGG ACAACAGGGC 
TGGCGCTGTG AGAACTGTCG CCGTCCTGGT GCTGAAGTTG ATGCAGACCT CATTCAGCCT 
CCAGTACGCA CTGATGCTTT CGACCGATAC AGGGAGAACG CTCTACGCAA ACTGAACATC 
CAGTGTCCCA TTGAGTGCCT GAGACCAGAC CTGTTAGCAG ATGCCCATAG CCCTCATGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1A ( XM_009304503.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302778.1 Danio rerio fibronectin 1a (fn1a), transcript variant X1, mRNA.

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CloneID	ODa48047
Clone ID Related Accession (Same CDS sequence)	XM_009304503.3 , XM_009304503.2
Accession Version	XM_009304503.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	fibronectin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304503.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGTG GCCCTTTGGG CACGGCGCTT GCGGTGGCAT TGCTTGCCTG CGCTGTGCAG 
TGTATGCCGA AAGGAACGGG GAAACATAAA AGACAAGCTC AGGAACACTC TGTATCATCT 
GTGTCACAAG ATGGATGTGT TATGGACGGA CAGTTTTATG GAGCTGGAGA GAAGTGGGAG 
CGCATTTATC TGGGCAGCAC GCTTCTTTGC ACTTGTCATG GAGTTTCTAA AATAGAGTGT 
AAATCAAAAC CTGATGCTGA GGAGACCTGC TACGATAAAG TCAATTCTCG TTCCTACCGG 
GTTGGAGAGA CTTACGAGAG GCCCAAGGAC GGAATGATCT GGGACTGTAC TTGCATTGGC 
TCTGCCCGAG GGAAAATCAG TTGCACCATT GCGAACCGCT GTCATGAGGG AGGGCACTCC 
TACAAGATTG GAGACACATG GCAGAGACCT CATGACACTG GAGACTACAT GCTGGAGTGT 
GTCTGCTTGG GAAATGGGAA GGGGGAGTGG ACCTGTAAAC CTGTTGCGGA GCGCTGTTAT 
GATAATACAG CTGGCACCTC CTACATGGTT GGTGAGACCT GGGAGAAGCC CTACCAGGGC 
TGGATGATTG TGGACTGCAC CTGCCTCGGA GAAGGAAGTG GACGTATTAC ATGCACATCC 
AGAAACCGCT GTAATGACCA GGACACACGC ACTTCTTACC GGATTGGGGA CACGTGGAGT 
AAGACGGACT CTCGTGGACA CGTTTTACAG TGTTTGTGTA CTGGTAATGG ACGTGGAGAG 
TGGAAGTGTG AGAGGCATGC TTCTCTGCAC ACTACTAGTC TTGGTACTGG GTCTCGTGTT 
GTGACCAATG TCCAGCCAGC AGTGTACCAC CCACAAGGGG TGCCTGAACA CCCTGTGGAG 
GGATCCTGTC TGACTGAAGC AGGTGTTTCC TATGCTCCAG GCATGCGCTG GAGCAAGGCT 
CAAGGTAGCA AGCAGATGTT GTGTACCTGC TTAGGAAACG GTGTCAGCTG TGAAGAGTCA 
GAGAGTCAAT CTCAGGTGTA TGGCGGGACC TCAGGTGGCG AACCGTGTGC TTTCCCTTTT 
GTGTTCATGG GGAAAACGTT TTACTCCTGC ACGTCTGAGG GACGCAATGA TGGACAGCTT 
TGGTGCAGCA CCTCATCTGA CTTTGAGAAA GACTACAAGT ATTCCTTCTG TACCAGCAAC 
AATGTTGTGG TCACAACCAG AGGAGGAAAC TCTAATGGTG CCCTGTGCCA GTTCCCTTTC 
CTCTACAATG GCCGTAACTA CACTGACTGC ACTGCTGATG GCAGAAGAGA TGGCATGAAA 
TGGTGCGGCA CGACTTATAA CTTTGACAAA GAGCAGCGCT TTGGCTTCTG TCCAATGGCA 
GCTCATGAAG AGGTGTGTAC CACCAATGAG GGTGTGATGT ATCGTGTGGG GGATCAATGG 
GACAGACGCC ATGATGTTCT GGGTCACATG ATGCGCTGTA CGTGTGTGGG CAATGGCCGT 
GGAGAATGGA GCTGCATCGC CTACTCTCAA CTCAAAGACC AGTGCATAGT GGATAATTTG 
ACCTATGAAG TAAATCAGAC TTTTACTAAA AGGCACGACG AAGGCTACAC AATGAACTGC 
ACCTGTTTCG GTCAAGGTCG TGGACGCTGG AAGTGTGATG CCATCGATCA GTGCCAGGAG 
CCGGAGACTA GAGTTTTCTA TCAGATTGGA GAGTCCTGGG ACAAACTCAT TCAGGGAATC 
CACTACAGAT GTTACTGCTA TGGTAATGGC ATCGGGGAGT TGAGCTGCGA GCCTCAGCAT 
TCTATCTCTG GTGGTCATCG TCCAGTCCAG GTCATTATTT CAGAAGCTGG AAATCAGCCT 
AACTCGCACC CCATCCAATG GAATGCACCA GCTTCTGCTC ACATCACACA GTACATACTT 
AAATGGAGGC CGAAAAACAC CCATATTCAG TGGATGGAGG TGACTATTCC AGGCCATGTC 
AACTCTTACA CTATTGCGGG ACTGAAGCCG GGAGTCACCT ACGAGGGTCA GCTCATCAGT 
ATCCTGCGTT TTGGACGTAG AGAGACCACT CGCTTTGATT TTTCCACCGT ACATGGCTCA 
TTGGAGACCT CTGAGGGTGA GACCACCCAG CCCCCTCGAA TGGTGGACAC CTCTGAGTCA 
GTGACTGAAA TCACCTCAAG CAGTTTCGTC ATCTCCTGGG TGTCTGCGTC TGACACCGTG 
TCTGGTTTTA GGGTGGAATA TGAGCTGAGT GAAGAGGGAG GCCAGACTGG ACAGCCCATG 
ATCTTGGACC TCCCTCATAG CGCTACGTCC GTGAACATCA GTGAACTTCT GCCAGGAAGG 
AAGTACACTG TGAATGTATA TGAAGTGACA GGAGAAGGAG AACCTAACCT CATCCTCACC 
ACTTCCCAGA CTACTGCTCC TGATGCTCCT TCTGATCATG AAGTGGAGGA TGTAGCAGAC 
ACCTCCATTC GTATCAGCTG GTCTAAACCT TTGGCTCCCA TCACAGGTTA CCGTGTAGTT 
TACACCCCAT CTGAAGAGGG CAGTTCTGGC AGTGTTGAGC TGAATCTGCC AGAACATTCC 
ACCTCATTGA CCCTTGGTGA TCTTCGACCT GGTGTGCTTT ATAACATCAG CATCTTCTCT 
GTGGAAGAGA ATCAGGAGAG TGAACCTGTT TTTGTTCAGG TCACCACTGC TGGAGAGCCA 
CTGGCGGAGG AAGTGCCGTC TCCAACCGAT CTGCAGTTTT ATGAGGTGTC CGACTCAAAG 
ATCACTATCA CCTGGACTTC TCCGTCCTCT GAGGTGTCTG GATACCGTGT GTCTGTGGGT 
GAAGTGGGTC CAGATGGCTT GATTGAGAAA GAAGTGCCTT ATCCAGTAAC ACAGAATGCT 
TATGCTGAAA TCACACACCT TCAGCCCGGC ACCCTCTACC GCTTCTTCAT CTTTTCCCTC 
AAATCAGGAG AGGAGAGCGA ACCGCTGGTG GGAGAGCAAG CTACAAAGCC GGATCCCCCA 
ACTGACATCT ACTTCTCCAA CATTACTGAA GACAGTGCTG TGATCGTCTG GCTGCCTCCC 
ACTGCTCAGA TCACAGGTTA CAGGCTTTTC CTGACAGTCG AGGGCTCCAA CCCCAAACAG 
CTGCGTATTC CAGGCCGTCT GTCGCAGTAC ACCCTCCTCA ACCTGCAGCC GGACACAGAA 
TACTCTGCCA CTCTGCATGC AGAAAGAGGA AACGTGCTCA GTGAAGGCTC CATTGCTACC 
TTCGCCACAA CTCCTCCAAT GGGAAATGCT CCGTACTTTA GCACTGATGT CACAGACACT 
TCTATCATTG TCTCCTGGAG CCCTCTGCCC AAAATTGGAT ACAAGTTGAC AGTGAGGCCC 
AGCCAAGGTG GAGAAGCCCC TAGAGATGTG ACCTCAGAGT TGGGTAGTGT CCTCATCTCA 
GGTCTGACCC CTGGAGTTGA GTACACATAC AGTGTCCAAC CAGTCATCAG TGGTCAAGAG 
CATGGAAACC CTATAACTCG GCGCGTGGTC ACTCCTCTTT CTCCACCCAC TGATCTGAAC 
CTGGAGTCCA ACCCCAACAC CGGAAAGCTA ACTGTTCAGT GGAATGATGC TAACATCCCA 
GACATCACGG GCTACAGAGT GACCTGCACC CCCACCAAAG GCCAGCAGGG CAATTCACTT 
GAGGAGTTTG TGAAGGCCGG ACAGAACTCA TGCACATTAG AGAACCTGAG TCCAGGAGTA 
GAGTACAATG TCAGCGTGTT CACTGTGAAG GACGACATGG AGAGCGTTCC TGTCTTCACA 
ACTGTAACCC CAGATGTGCC TAAAATAACT GATCTCAGCT TCATCAATGT CACTGACTCT 
ACTATTGGCC TAAGCTGGAG TCCTCTAAAC TCCACTGCCG TTACCGGCTA CCGCATCACA 
GTTTTGGCAG CTGGGGACAG TGTCCCGATC TTTGTTGAAT TTGTGGAGCC CACAACAGGC 
TTTTATACTG TTCATGGCCT GGAGCCAGGC ATAGATTATG ACATCACCGT CACCACAGTT 
ACCGAAAATG GAGAGAGCGA ACCCATCACT ATCACCCAGC AAACAGCTGT CCCGGCTCCA 
TATGGCCTGT CATTTGGAGA AGTGACCGCA GACACTATGT TGGTGACCTG GAAAGCCCCT 
CAGGTTCCCA AATCCTCAGA CATTAATCAA TACATCATCC GTTATCATCC AGTCGATGAA 
GATGACGAAA CTACTGAACG CACTGTGGAA GGAAGTGAAA ACTTTGTTGT CCTTCGCCAT 
CTTGTGCCCA ACACAGAGTA CCTGGTGAGT GTGATTTGCG TATATGAGGG CAGAGAGGGA 
TCACCTGCCA TCGGCACCCA GAGAACTGTT TTTGATGCCC CTGTTGGCCT TCAGTTCTCT 
GATGTGGGCA CCACCTCCTT CACTGTGCGC TGGCAGGCTC CTCGGGCCAT CATCTCAGGA 
TACCGTATCC GCTACCAGAT GACCAGTGGT GGACGAGCTA AAGAGGAGAG ACTGCCCCCC 
TCGAGGAGCC ACTTCACCCT GACAGGCCTG ACCCCAGAGA CTGAGTACAG CATCAGTGTC 
TATGCCGTCA GCGGATCCAG AGACAGTCTG CCTCTGACTG GAACTCAGTC TACCATCTCT 
GATGCTCCTA CTGACCTGGA AGTGATTTCC TCAACGCCGA CCAGCATCAC CGTTCGCTGG 
GATGCCCCCT CTGTCACTGT GCGGTACTAC AGGATTACAC ATGGAGAGTC GGGTGGTTCT 
GATGCCCCTC TGGAATTTAT GGTTCCCGGC TCCCAGTCTA CAGCCACTAT TGAAGATCTA 
CGTCCAGGCA CTGATTATAC CATTACTGTT TATGCTGTGA CCGGTAGAGG GGACAGCCCT 
GCATCCAGCA CTCCCATCCA TGTTAAACAT AGAACCGATA TTGACTCTCC ATCACAGATG 
GAGGTGACTG ACGTGAAGGA CAACACTATT ACTGTACGCT GGACCCCAGC AGCCGGCCCC 
ATCTCAAGAT ACAGAGTCAA TGCAACTCCT CTTACTGGAG AAGGACCTGT GTTACATGCA 
GAAACGACTT CAGACCATAC TGAAATTACG TTTTCTGGCC TGATGCCTGC AGTGGAGTAC 
AGCATCAAGG TGTATGCTCT GGGTCAGGAT GGAGAGAGCC TCCCTCTGGT CGAAAATGTA 
GTTACAATGG ACAAGCCCAA GGATCTGTCC TTCACTGACG TGGAATCCAG CTCTATGCGC 
ATCTCCTGGG ACAGTCCCGA TGGCGTGGTC TCCTCCTACA GAGTGTTGTA CTACAGTCCA 
GAGGAGGGAG AGAGAGAGCT GTTTCCTGCC CCGCATGGAG ATGACGAGTC TGCAGTACTT 
CATGGCCTCA GACCCGGCAC AGAATATACT GTTAAAGTCA TCGCACTGCA TGACCAATCA 
CCCAGCACTG CACTGGTCGG CACTCAGACA ACAGGCATTC CTGGACCCAC AAGACTGCAG 
TTTTCCCAGG TGGGCCCAAC ATCCTTCACA GTCTCCTGGT CTTCATCTGA CGCCAGTTTG 
ACAGGTTATC GTGTGGCTGT TTCACCCAAG AGCAAAAGTG GCCCTACTAA AGAAGAAAAC 
ATCACTCCTG ACTCCACTGA GTTCCACGCC ACTGGACTCA TGCCTGGCAC TGATTACGAA 
GTGGAGGTTT ATGGTGTGAA GAACTCTCTA ACCAGCCCTC GTGTCAAAGG AGAAATCACA 
ACACCTGACA ATATCAGTCC ACCTCGACGT GTCCGTATAT CCAATGTGAA AGACTCCTCC 
ATCACTCTGA CCTGGCGCTC AAAGACTGAG GCCATTTCTG GCTTCTTGGT GGAAGCAACT 
CCCACCATGG GTGGCCATAA TCCAATCCAG AGGACCATTG AACCTGATTC ACGCACCTAC 
ACAATTGCTG GTCTGGAGCC AGGAACTAAT TATAAGATCA ATATTTATAC TCTGAATGGA 
AACTCTCGTA GTGAGCCTTT CACCCTTACA GCTACTACAG CAAAACCAGT TATTAGCCCA 
CCTACAAACC TCCATTTTAC TTCTCTGGCT TCTACTTCAA TCTCCTTCAC CTGGGAGCCT 
CCACGCAGCA CTATTACTGG ATATTATGTG ACCTATGAGG AGGCTGGAGG CATTCCCAAA 
GAGCTGACCC CTCGACCCCA GGCTGCCAGA ACCTTCGCGT CCATCTCTGG GTTGATACCA 
GGCACTGAGT ACATTATCAA GATTGTTGCA TTAAATGGCG CTCAGAGAAG CACGCCATTG 
ATTGGAAAGG CAAGAACTCA ACTGGAAATT GAGCAGCTTC CCCCTCAGCT ACCTAATCCC 
AGCCGACCCC ATCTCGAAGT CTTGGATGTG CCCGAGGACA ATGACAACTT CAACAACCAC 
GTACATGTGC TGGGGCCAAA CAGACACAAC ACACTGGGGC AGCAGGGTCA ACACATCTAC 
ACCGAATATC AGAGCTTCAA CCTGGGCAAC AACGGCCAGC AGCCTCATCA TCCAAACCAC 
GGCGAGCCGC TAGTCTACAT CCCTCTGCCG GGAGCGGACG GCCAGAGAGT GCCTGTGGTG 
AAGGTCAACG AGGGTCAAGA AGCAGGTTTC CCCTTTGGCG GCCTTTTTAA TGAGACAAAC 
CTGCCCCAGG AATCTCAGAC TCAGACTACA ATTGTGTGGC AGCCTGTGCC ACACACGTCT 
GAGTATGTGG TTTGGTGCGA CCCCATTACT GAGATCAATG AGAAGAGCTT CCAGATGCGT 
CTGCCTGGTA CATCAACAAG TGCTACCCTG ATTGGCCTTA CCTCAGGTGC CTCCTATAAT 
GTTCTTGTGG AGTCTGTTAA TGGAGACCAA AAACAGAAAG TGCTGCAAGA CGTTGTGACA 
GTAGGAAATA GCGTTCCAGG AGCTGGAGTG ATTCCCACAG GCAGGGATGT GTGTTATGAT 
TCATTCACCG CCACCTATCA TGAGGTTAAC ACGGAGTGGG AACGCATGTC CGAGACAGGC 
TTTAAACTTT GGTGCAAGTG CCTGGGCCTT GGCAGTGGTC ATTTCAGATG TGATTCATCC 
AAATGGTGCC ATGACAGTGG CCACAACTAC CGAATCGGTG AGAAATGGGA CCGGCGTGCT 
GAGAACGGCC ACATGATGAG CTGTACATGT CTGGGAAATG GGAAAGGAGA GTTTAAGTGT 
GAACCTCATG AATCCACCTG TTATGACGAG GGGAAATTGT ACCAGGTGGG CAACCAGTGG 
CAAAAGGAGT ATCTTGGAGC CATTTGCACA TGTACCTGCT ACGGAGGACA ACAGGGCTGG 
CGCTGTGAGA ACTGTCGCCG TCCTGGTGCT GAAGTTGATG CAGACCTCAT TCAGCCTCCA 
GTACGCACTG ATGCTTTCGA CCGATACAGG GAGAACGCTC TACGCAAACT GAACATCCAG 
TGTCCCATTG AGTGCCTGAG ACCAGACCTG TTAGCAGATG CCCATAGCCC TCATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302778.1
CDS	175..7614
Translation	MFGGPLGTALAVALLACAVQCMPKGTGKHKRQAQEHSVSSVSQDGCVMDGQFYGAGEKWERIYLGSTLLCTCHGVSKIECKSKPDAEETCYDKVNSRSYRVGETYERPKDGMIWDCTCIGSARGKISCTIANRCHEGGHSYKIGDTWQRPHDTGDYMLECVCLGNGKGEWTCKPVAERCYDNTAGTSYMVGETWEKPYQGWMIVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKTDSRGHVLQCLCTGNGRGEWKCERHASLHTTSLGTGSRVVTNVQPAVYHPQGVPEHPVEGSCLTEAGVSYAPGMRWSKAQGSKQMLCTCLGNGVSCEESESQSQVYGGTSGGEPCAFPFVFMGKTFYSCTSEGRNDGQLWCSTSSDFEKDYKYSFCTSNNVVVTTRGGNSNGALCQFPFLYNGRNYTDCTADGRRDGMKWCGTTYNFDKEQRFGFCPMAAHEEVCTTNEGVMYRVGDQWDRRHDVLGHMMRCTCVGNGRGEWSCIAYSQLKDQCIVDNLTYEVNQTFTKRHDEGYTMNCTCFGQGRGRWKCDAIDQCQEPETRVFYQIGESWDKLIQGIHYRCYCYGNGIGELSCEPQHSISGGHRPVQVIISEAGNQPNSHPIQWNAPASAHITQYILKWRPKNTHIQWMEVTIPGHVNSYTIAGLKPGVTYEGQLISILRFGRRETTRFDFSTVHGSLETSEGETTQPPRMVDTSESVTEITSSSFVISWVSASDTVSGFRVEYELSEEGGQTGQPMILDLPHSATSVNISELLPGRKYTVNVYEVTGEGEPNLILTTSQTTAPDAPSDHEVEDVADTSIRISWSKPLAPITGYRVVYTPSEEGSSGSVELNLPEHSTSLTLGDLRPGVLYNISIFSVEENQESEPVFVQVTTAGEPLAEEVPSPTDLQFYEVSDSKITITWTSPSSEVSGYRVSVGEVGPDGLIEKEVPYPVTQNAYAEITHLQPGTLYRFFIFSLKSGEESEPLVGEQATKPDPPTDIYFSNITEDSAVIVWLPPTAQITGYRLFLTVEGSNPKQLRIPGRLSQYTLLNLQPDTEYSATLHAERGNVLSEGSIATFATTPPMGNAPYFSTDVTDTSIIVSWSPLPKIGYKLTVRPSQGGEAPRDVTSELGSVLISGLTPGVEYTYSVQPVISGQEHGNPITRRVVTPLSPPTDLNLESNPNTGKLTVQWNDANIPDITGYRVTCTPTKGQQGNSLEEFVKAGQNSCTLENLSPGVEYNVSVFTVKDDMESVPVFTTVTPDVPKITDLSFINVTDSTIGLSWSPLNSTAVTGYRITVLAAGDSVPIFVEFVEPTTGFYTVHGLEPGIDYDITVTTVTENGESEPITITQQTAVPAPYGLSFGEVTADTMLVTWKAPQVPKSSDINQYIIRYHPVDEDDETTERTVEGSENFVVLRHLVPNTEYLVSVICVYEGREGSPAIGTQRTVFDAPVGLQFSDVGTTSFTVRWQAPRAIISGYRIRYQMTSGGRAKEERLPPSRSHFTLTGLTPETEYSISVYAVSGSRDSLPLTGTQSTISDAPTDLEVISSTPTSITVRWDAPSVTVRYYRITHGESGGSDAPLEFMVPGSQSTATIEDLRPGTDYTITVYAVTGRGDSPASSTPIHVKHRTDIDSPSQMEVTDVKDNTITVRWTPAAGPISRYRVNATPLTGEGPVLHAETTSDHTEITFSGLMPAVEYSIKVYALGQDGESLPLVENVVTMDKPKDLSFTDVESSSMRISWDSPDGVVSSYRVLYYSPEEGERELFPAPHGDDESAVLHGLRPGTEYTVKVIALHDQSPSTALVGTQTTGIPGPTRLQFSQVGPTSFTVSWSSSDASLTGYRVAVSPKSKSGPTKEENITPDSTEFHATGLMPGTDYEVEVYGVKNSLTSPRVKGEITTPDNISPPRRVRISNVKDSSITLTWRSKTEAISGFLVEATPTMGGHNPIQRTIEPDSRTYTIAGLEPGTNYKINIYTLNGNSRSEPFTLTATTAKPVISPPTNLHFTSLASTSISFTWEPPRSTITGYYVTYEEAGGIPKELTPRPQAARTFASISGLIPGTEYIIKIVALNGAQRSTPLIGKARTQLEIEQLPPQLPNPSRPHLEVLDVPEDNDNFNNHVHVLGPNRHNTLGQQGQHIYTEYQSFNLGNNGQQPHHPNHGEPLVYIPLPGADGQRVPVVKVNEGQEAGFPFGGLFNETNLPQESQTQTTIVWQPVPHTSEYVVWCDPITEINEKSFQMRLPGTSTSATLIGLTSGASYNVLVESVNGDQKQKVLQDVVTVGNSVPGAGVIPTGRDVCYDSFTATYHEVNTEWERMSETGFKLWCKCLGLGSGHFRCDSSKWCHDSGHNYRIGEKWDRRAENGHMMSCTCLGNGKGEFKCEPHESTCYDEGKLYQVGNQWQKEYLGAICTCTCYGGQQGWRCENCRRPGAEVDADLIQPPVRTDAFDRYRENALRKLNIQCPIECLRPDLLADAHSPHD

Target ORF information:

RefSeq Version	XM_009304503.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fibronectin 1a (fn1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304503.3

ORF Insert Sequence:

ATGTTTGGTG GCCCTTTGGG CACGGCGCTT GCGGTGGCAT TGCTTGCCTG CGCTGTGCAG 
TGTATGCCGA AAGGAACGGG GAAACATAAA AGACAAGCTC AGGAACACTC TGTATCATCT 
GTGTCACAAG ATGGATGTGT TATGGACGGA CAGTTTTATG GAGCTGGAGA GAAGTGGGAG 
CGCATTTATC TGGGCAGCAC GCTTCTTTGC ACTTGTCATG GAGTTTCTAA AATAGAGTGT 
AAATCAAAAC CTGATGCTGA GGAGACCTGC TACGATAAAG TCAATTCTCG TTCCTACCGG 
GTTGGAGAGA CTTACGAGAG GCCCAAGGAC GGAATGATCT GGGACTGTAC TTGCATTGGC 
TCTGCCCGAG GGAAAATCAG TTGCACCATT GCGAACCGCT GTCATGAGGG AGGGCACTCC 
TACAAGATTG GAGACACATG GCAGAGACCT CATGACACTG GAGACTACAT GCTGGAGTGT 
GTCTGCTTGG GAAATGGGAA GGGGGAGTGG ACCTGTAAAC CTGTTGCGGA GCGCTGTTAT 
GATAATACAG CTGGCACCTC CTACATGGTT GGTGAGACCT GGGAGAAGCC CTACCAGGGC 
TGGATGATTG TGGACTGCAC CTGCCTCGGA GAAGGAAGTG GACGTATTAC ATGCACATCC 
AGAAACCGCT GTAATGACCA GGACACACGC ACTTCTTACC GGATTGGGGA CACGTGGAGT 
AAGACGGACT CTCGTGGACA CGTTTTACAG TGTTTGTGTA CTGGTAATGG ACGTGGAGAG 
TGGAAGTGTG AGAGGCATGC TTCTCTGCAC ACTACTAGTC TTGGTACTGG GTCTCGTGTT 
GTGACCAATG TCCAGCCAGC AGTGTACCAC CCACAAGGGG TGCCTGAACA CCCTGTGGAG 
GGATCCTGTC TGACTGAAGC AGGTGTTTCC TATGCTCCAG GCATGCGCTG GAGCAAGGCT 
CAAGGTAGCA AGCAGATGTT GTGTACCTGC TTAGGAAACG GTGTCAGCTG TGAAGAGTCA 
GAGAGTCAAT CTCAGGTGTA TGGCGGGACC TCAGGTGGCG AACCGTGTGC TTTCCCTTTT 
GTGTTCATGG GGAAAACGTT TTACTCCTGC ACGTCTGAGG GACGCAATGA TGGACAGCTT 
TGGTGCAGCA CCTCATCTGA CTTTGAGAAA GACTACAAGT ATTCCTTCTG TACCAGCAAC 
AATGTTGTGG TCACAACCAG AGGAGGAAAC TCTAATGGTG CCCTGTGCCA GTTCCCTTTC 
CTCTACAATG GCCGTAACTA CACTGACTGC ACTGCTGATG GCAGAAGAGA TGGCATGAAA 
TGGTGCGGCA CGACTTATAA CTTTGACAAA GAGCAGCGCT TTGGCTTCTG TCCAATGGCA 
GCTCATGAAG AGGTGTGTAC CACCAATGAG GGTGTGATGT ATCGTGTGGG GGATCAATGG 
GACAGACGCC ATGATGTTCT GGGTCACATG ATGCGCTGTA CGTGTGTGGG CAATGGCCGT 
GGAGAATGGA GCTGCATCGC CTACTCTCAA CTCAAAGACC AGTGCATAGT GGATAATTTG 
ACCTATGAAG TAAATCAGAC TTTTACTAAA AGGCACGACG AAGGCTACAC AATGAACTGC 
ACCTGTTTCG GTCAAGGTCG TGGACGCTGG AAGTGTGATG CCATCGATCA GTGCCAGGAG 
CCGGAGACTA GAGTTTTCTA TCAGATTGGA GAGTCCTGGG ACAAACTCAT TCAGGGAATC 
CACTACAGAT GTTACTGCTA TGGTAATGGC ATCGGGGAGT TGAGCTGCGA GCCTCAGCAT 
TCTATCTCTG GTGGTCATCG TCCAGTCCAG GTCATTATTT CAGAAGCTGG AAATCAGCCT 
AACTCGCACC CCATCCAATG GAATGCACCA GCTTCTGCTC ACATCACACA GTACATACTT 
AAATGGAGGC CGAAAAACAC CCATATTCAG TGGATGGAGG TGACTATTCC AGGCCATGTC 
AACTCTTACA CTATTGCGGG ACTGAAGCCG GGAGTCACCT ACGAGGGTCA GCTCATCAGT 
ATCCTGCGTT TTGGACGTAG AGAGACCACT CGCTTTGATT TTTCCACCGT ACATGGCTCA 
TTGGAGACCT CTGAGGGTGA GACCACCCAG CCCCCTCGAA TGGTGGACAC CTCTGAGTCA 
GTGACTGAAA TCACCTCAAG CAGTTTCGTC ATCTCCTGGG TGTCTGCGTC TGACACCGTG 
TCTGGTTTTA GGGTGGAATA TGAGCTGAGT GAAGAGGGAG GCCAGACTGG ACAGCCCATG 
ATCTTGGACC TCCCTCATAG CGCTACGTCC GTGAACATCA GTGAACTTCT GCCAGGAAGG 
AAGTACACTG TGAATGTATA TGAAGTGACA GGAGAAGGAG AACCTAACCT CATCCTCACC 
ACTTCCCAGA CTACTGCTCC TGATGCTCCT TCTGATCATG AAGTGGAGGA TGTAGCAGAC 
ACCTCCATTC GTATCAGCTG GTCTAAACCT TTGGCTCCCA TCACAGGTTA CCGTGTAGTT 
TACACCCCAT CTGAAGAGGG CAGTTCTGGC AGTGTTGAGC TGAATCTGCC AGAACATTCC 
ACCTCATTGA CCCTTGGTGA TCTTCGACCT GGTGTGCTTT ATAACATCAG CATCTTCTCT 
GTGGAAGAGA ATCAGGAGAG TGAACCTGTT TTTGTTCAGG TCACCACTGC TGGAGAGCCA 
CTGGCGGAGG AAGTGCCGTC TCCAACCGAT CTGCAGTTTT ATGAGGTGTC CGACTCAAAG 
ATCACTATCA CCTGGACTTC TCCGTCCTCT GAGGTGTCTG GATACCGTGT GTCTGTGGGT 
GAAGTGGGTC CAGATGGCTT GATTGAGAAA GAAGTGCCTT ATCCAGTAAC ACAGAATGCT 
TATGCTGAAA TCACACACCT TCAGCCCGGC ACCCTCTACC GCTTCTTCAT CTTTTCCCTC 
AAATCAGGAG AGGAGAGCGA ACCGCTGGTG GGAGAGCAAG CTACAAAGCC GGATCCCCCA 
ACTGACATCT ACTTCTCCAA CATTACTGAA GACAGTGCTG TGATCGTCTG GCTGCCTCCC 
ACTGCTCAGA TCACAGGTTA CAGGCTTTTC CTGACAGTCG AGGGCTCCAA CCCCAAACAG 
CTGCGTATTC CAGGCCGTCT GTCGCAGTAC ACCCTCCTCA ACCTGCAGCC GGACACAGAA 
TACTCTGCCA CTCTGCATGC AGAAAGAGGA AACGTGCTCA GTGAAGGCTC CATTGCTACC 
TTCGCCACAA CTCCTCCAAT GGGAAATGCT CCGTACTTTA GCACTGATGT CACAGACACT 
TCTATCATTG TCTCCTGGAG CCCTCTGCCC AAAATTGGAT ACAAGTTGAC AGTGAGGCCC 
AGCCAAGGTG GAGAAGCCCC TAGAGATGTG ACCTCAGAGT TGGGTAGTGT CCTCATCTCA 
GGTCTGACCC CTGGAGTTGA GTACACATAC AGTGTCCAAC CAGTCATCAG TGGTCAAGAG 
CATGGAAACC CTATAACTCG GCGCGTGGTC ACTCCTCTTT CTCCACCCAC TGATCTGAAC 
CTGGAGTCCA ACCCCAACAC CGGAAAGCTA ACTGTTCAGT GGAATGATGC TAACATCCCA 
GACATCACGG GCTACAGAGT GACCTGCACC CCCACCAAAG GCCAGCAGGG CAATTCACTT 
GAGGAGTTTG TGAAGGCCGG ACAGAACTCA TGCACATTAG AGAACCTGAG TCCAGGAGTA 
GAGTACAATG TCAGCGTGTT CACTGTGAAG GACGACATGG AGAGCGTTCC TGTCTTCACA 
ACTGTAACCC CAGATGTGCC TAAAATAACT GATCTCAGCT TCATCAATGT CACTGACTCT 
ACTATTGGCC TAAGCTGGAG TCCTCTAAAC TCCACTGCCG TTACCGGCTA CCGCATCACA 
GTTTTGGCAG CTGGGGACAG TGTCCCGATC TTTGTTGAAT TTGTGGAGCC CACAACAGGC 
TTTTATACTG TTCATGGCCT GGAGCCAGGC ATAGATTATG ACATCACCGT CACCACAGTT 
ACCGAAAATG GAGAGAGCGA ACCCATCACT ATCACCCAGC AAACAGCTGT CCCGGCTCCA 
TATGGCCTGT CATTTGGAGA AGTGACCGCA GACACTATGT TGGTGACCTG GAAAGCCCCT 
CAGGTTCCCA AATCCTCAGA CATTAATCAA TACATCATCC GTTATCATCC AGTCGATGAA 
GATGACGAAA CTACTGAACG CACTGTGGAA GGAAGTGAAA ACTTTGTTGT CCTTCGCCAT 
CTTGTGCCCA ACACAGAGTA CCTGGTGAGT GTGATTTGCG TATATGAGGG CAGAGAGGGA 
TCACCTGCCA TCGGCACCCA GAGAACTGTT TTTGATGCCC CTGTTGGCCT TCAGTTCTCT 
GATGTGGGCA CCACCTCCTT CACTGTGCGC TGGCAGGCTC CTCGGGCCAT CATCTCAGGA 
TACCGTATCC GCTACCAGAT GACCAGTGGT GGACGAGCTA AAGAGGAGAG ACTGCCCCCC 
TCGAGGAGCC ACTTCACCCT GACAGGCCTG ACCCCAGAGA CTGAGTACAG CATCAGTGTC 
TATGCCGTCA GCGGATCCAG AGACAGTCTG CCTCTGACTG GAACTCAGTC TACCATCTCT 
GATGCTCCTA CTGACCTGGA AGTGATTTCC TCAACGCCGA CCAGCATCAC CGTTCGCTGG 
GATGCCCCCT CTGTCACTGT GCGGTACTAC AGGATTACAC ATGGAGAGTC GGGTGGTTCT 
GATGCCCCTC TGGAATTTAT GGTTCCCGGC TCCCAGTCTA CAGCCACTAT TGAAGATCTA 
CGTCCAGGCA CTGATTATAC CATTACTGTT TATGCTGTGA CCGGTAGAGG GGACAGCCCT 
GCATCCAGCA CTCCCATCCA TGTTAAACAT AGAACCGATA TTGACTCTCC ATCACAGATG 
GAGGTGACTG ACGTGAAGGA CAACACTATT ACTGTACGCT GGACCCCAGC AGCCGGCCCC 
ATCTCAAGAT ACAGAGTCAA TGCAACTCCT CTTACTGGAG AAGGACCTGT GTTACATGCA 
GAAACGACTT CAGACCATAC TGAAATTACG TTTTCTGGCC TGATGCCTGC AGTGGAGTAC 
AGCATCAAGG TGTATGCTCT GGGTCAGGAT GGAGAGAGCC TCCCTCTGGT CGAAAATGTA 
GTTACAATGG ACAAGCCCAA GGATCTGTCC TTCACTGACG TGGAATCCAG CTCTATGCGC 
ATCTCCTGGG ACAGTCCCGA TGGCGTGGTC TCCTCCTACA GAGTGTTGTA CTACAGTCCA 
GAGGAGGGAG AGAGAGAGCT GTTTCCTGCC CCGCATGGAG ATGACGAGTC TGCAGTACTT 
CATGGCCTCA GACCCGGCAC AGAATATACT GTTAAAGTCA TCGCACTGCA TGACCAATCA 
CCCAGCACTG CACTGGTCGG CACTCAGACA ACAGGCATTC CTGGACCCAC AAGACTGCAG 
TTTTCCCAGG TGGGCCCAAC ATCCTTCACA GTCTCCTGGT CTTCATCTGA CGCCAGTTTG 
ACAGGTTATC GTGTGGCTGT TTCACCCAAG AGCAAAAGTG GCCCTACTAA AGAAGAAAAC 
ATCACTCCTG ACTCCACTGA GTTCCACGCC ACTGGACTCA TGCCTGGCAC TGATTACGAA 
GTGGAGGTTT ATGGTGTGAA GAACTCTCTA ACCAGCCCTC GTGTCAAAGG AGAAATCACA 
ACACCTGACA ATATCAGTCC ACCTCGACGT GTCCGTATAT CCAATGTGAA AGACTCCTCC 
ATCACTCTGA CCTGGCGCTC AAAGACTGAG GCCATTTCTG GCTTCTTGGT GGAAGCAACT 
CCCACCATGG GTGGCCATAA TCCAATCCAG AGGACCATTG AACCTGATTC ACGCACCTAC 
ACAATTGCTG GTCTGGAGCC AGGAACTAAT TATAAGATCA ATATTTATAC TCTGAATGGA 
AACTCTCGTA GTGAGCCTTT CACCCTTACA GCTACTACAG CAAAACCAGT TATTAGCCCA 
CCTACAAACC TCCATTTTAC TTCTCTGGCT TCTACTTCAA TCTCCTTCAC CTGGGAGCCT 
CCACGCAGCA CTATTACTGG ATATTATGTG ACCTATGAGG AGGCTGGAGG CATTCCCAAA 
GAGCTGACCC CTCGACCCCA GGCTGCCAGA ACCTTCGCGT CCATCTCTGG GTTGATACCA 
GGCACTGAGT ACATTATCAA GATTGTTGCA TTAAATGGCG CTCAGAGAAG CACGCCATTG 
ATTGGAAAGG CAAGAACTCA ACTGGAAATT GAGCAGCTTC CCCCTCAGCT ACCTAATCCC 
AGCCGACCCC ATCTCGAAGT CTTGGATGTG CCCGAGGACA ATGACAACTT CAACAACCAC 
GTACATGTGC TGGGGCCAAA CAGACACAAC ACACTGGGGC AGCAGGGTCA ACACATCTAC 
ACCGAATATC AGAGCTTCAA CCTGGGCAAC AACGGCCAGC AGCCTCATCA TCCAAACCAC 
GGCGAGCCGC TAGTCTACAT CCCTCTGCCG GGAGCGGACG GCCAGAGAGT GCCTGTGGTG 
AAGGTCAACG AGGGTCAAGA AGCAGGTTTC CCCTTTGGCG GCCTTTTTAA TGAGACAAAC 
CTGCCCCAGG AATCTCAGAC TCAGACTACA ATTGTGTGGC AGCCTGTGCC ACACACGTCT 
GAGTATGTGG TTTGGTGCGA CCCCATTACT GAGATCAATG AGAAGAGCTT CCAGATGCGT 
CTGCCTGGTA CATCAACAAG TGCTACCCTG ATTGGCCTTA CCTCAGGTGC CTCCTATAAT 
GTTCTTGTGG AGTCTGTTAA TGGAGACCAA AAACAGAAAG TGCTGCAAGA CGTTGTGACA 
GTAGGAAATA GCGTTCCAGG AGCTGGAGTG ATTCCCACAG GCAGGGATGT GTGTTATGAT 
TCATTCACCG CCACCTATCA TGAGGTTAAC ACGGAGTGGG AACGCATGTC CGAGACAGGC 
TTTAAACTTT GGTGCAAGTG CCTGGGCCTT GGCAGTGGTC ATTTCAGATG TGATTCATCC 
AAATGGTGCC ATGACAGTGG CCACAACTAC CGAATCGGTG AGAAATGGGA CCGGCGTGCT 
GAGAACGGCC ACATGATGAG CTGTACATGT CTGGGAAATG GGAAAGGAGA GTTTAAGTGT 
GAACCTCATG AATCCACCTG TTATGACGAG GGGAAATTGT ACCAGGTGGG CAACCAGTGG 
CAAAAGGAGT ATCTTGGAGC CATTTGCACA TGTACCTGCT ACGGAGGACA ACAGGGCTGG 
CGCTGTGAGA ACTGTCGCCG TCCTGGTGCT GAAGTTGATG CAGACCTCAT TCAGCCTCCA 
GTACGCACTG ATGCTTTCGA CCGATACAGG GAGAACGCTC TACGCAAACT GAACATCCAG 
TGTCCCATTG AGTGCCTGAG ACCAGACCTG TTAGCAGATG CCCATAGCCC TCATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FN1A ( XM_009304503.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302778.1 Danio rerio fibronectin 1a (fn1a), transcript variant X1, mRNA.

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CloneID	ODa48047
Clone ID Related Accession (Same CDS sequence)	XM_009304503.3 , XM_009304503.2
Accession Version	XM_009304503.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	fibronectin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGTG GCCCTTTGGG CACGGCGCTT GCGGTGGCAT TGCTTGCCTG CGCTGTGCAG 
TGTATGCCGA AAGGAACGGG GAAACATAAA AGACAAGCTC AGGAACACTC TGTATCATCT 
GTGTCACAAG ATGGATGTGT TATGGACGGA CAGTTTTATG GAGCTGGAGA GAAGTGGGAG 
CGCATTTATC TGGGCAGCAC GCTTCTTTGC ACTTGTCATG GAGTTTCTAA AATAGAGTGT 
AAATCAAAAC CTGATGCTGA GGAGACCTGC TACGATAAAG TCAATTCTCG TTCCTACCGG 
GTTGGAGAGA CTTACGAGAG GCCCAAGGAC GGAATGATCT GGGACTGTAC TTGCATTGGC 
TCTGCCCGAG GGAAAATCAG TTGCACCATT GCGAACCGCT GTCATGAGGG AGGGCACTCC 
TACAAGATTG GAGACACATG GCAGAGACCT CATGACACTG GAGACTACAT GCTGGAGTGT 
GTCTGCTTGG GAAATGGGAA GGGGGAGTGG ACCTGTAAAC CTGTTGCGGA GCGCTGTTAT 
GATAATACAG CTGGCACCTC CTACATGGTT GGTGAGACCT GGGAGAAGCC CTACCAGGGC 
TGGATGATTG TGGACTGCAC CTGCCTCGGA GAAGGAAGTG GACGTATTAC ATGCACATCC 
AGAAACCGCT GTAATGACCA GGACACACGC ACTTCTTACC GGATTGGGGA CACGTGGAGT 
AAGACGGACT CTCGTGGACA CGTTTTACAG TGTTTGTGTA CTGGTAATGG ACGTGGAGAG 
TGGAAGTGTG AGAGGCATGC TTCTCTGCAC ACTACTAGTC TTGGTACTGG GTCTCGTGTT 
GTGACCAATG TCCAGCCAGC AGTGTACCAC CCACAAGGGG TGCCTGAACA CCCTGTGGAG 
GGATCCTGTC TGACTGAAGC AGGTGTTTCC TATGCTCCAG GCATGCGCTG GAGCAAGGCT 
CAAGGTAGCA AGCAGATGTT GTGTACCTGC TTAGGAAACG GTGTCAGCTG TGAAGAGTCA 
GAGAGTCAAT CTCAGGTGTA TGGCGGGACC TCAGGTGGCG AACCGTGTGC TTTCCCTTTT 
GTGTTCATGG GGAAAACGTT TTACTCCTGC ACGTCTGAGG GACGCAATGA TGGACAGCTT 
TGGTGCAGCA CCTCATCTGA CTTTGAGAAA GACTACAAGT ATTCCTTCTG TACCAGCAAC 
AATGTTGTGG TCACAACCAG AGGAGGAAAC TCTAATGGTG CCCTGTGCCA GTTCCCTTTC 
CTCTACAATG GCCGTAACTA CACTGACTGC ACTGCTGATG GCAGAAGAGA TGGCATGAAA 
TGGTGCGGCA CGACTTATAA CTTTGACAAA GAGCAGCGCT TTGGCTTCTG TCCAATGGCA 
GCTCATGAAG AGGTGTGTAC CACCAATGAG GGTGTGATGT ATCGTGTGGG GGATCAATGG 
GACAGACGCC ATGATGTTCT GGGTCACATG ATGCGCTGTA CGTGTGTGGG CAATGGCCGT 
GGAGAATGGA GCTGCATCGC CTACTCTCAA CTCAAAGACC AGTGCATAGT GGATAATTTG 
ACCTATGAAG TAAATCAGAC TTTTACTAAA AGGCACGACG AAGGCTACAC AATGAACTGC 
ACCTGTTTCG GTCAAGGTCG TGGACGCTGG AAGTGTGATG CCATCGATCA GTGCCAGGAG 
CCGGAGACTA GAGTTTTCTA TCAGATTGGA GAGTCCTGGG ACAAACTCAT TCAGGGAATC 
CACTACAGAT GTTACTGCTA TGGTAATGGC ATCGGGGAGT TGAGCTGCGA GCCTCAGCAT 
TCTATCTCTG GTGGTCATCG TCCAGTCCAG GTCATTATTT CAGAAGCTGG AAATCAGCCT 
AACTCGCACC CCATCCAATG GAATGCACCA GCTTCTGCTC ACATCACACA GTACATACTT 
AAATGGAGGC CGAAAAACAC CCATATTCAG TGGATGGAGG TGACTATTCC AGGCCATGTC 
AACTCTTACA CTATTGCGGG ACTGAAGCCG GGAGTCACCT ACGAGGGTCA GCTCATCAGT 
ATCCTGCGTT TTGGACGTAG AGAGACCACT CGCTTTGATT TTTCCACCGT ACATGGCTCA 
TTGGAGACCT CTGAGGGTGA GACCACCCAG CCCCCTCGAA TGGTGGACAC CTCTGAGTCA 
GTGACTGAAA TCACCTCAAG CAGTTTCGTC ATCTCCTGGG TGTCTGCGTC TGACACCGTG 
TCTGGTTTTA GGGTGGAATA TGAGCTGAGT GAAGAGGGAG GCCAGACTGG ACAGCCCATG 
ATCTTGGACC TCCCTCATAG CGCTACGTCC GTGAACATCA GTGAACTTCT GCCAGGAAGG 
AAGTACACTG TGAATGTATA TGAAGTGACA GGAGAAGGAG AACCTAACCT CATCCTCACC 
ACTTCCCAGA CTACTGCTCC TGATGCTCCT TCTGATCATG AAGTGGAGGA TGTAGCAGAC 
ACCTCCATTC GTATCAGCTG GTCTAAACCT TTGGCTCCCA TCACAGGTTA CCGTGTAGTT 
TACACCCCAT CTGAAGAGGG CAGTTCTGGC AGTGTTGAGC TGAATCTGCC AGAACATTCC 
ACCTCATTGA CCCTTGGTGA TCTTCGACCT GGTGTGCTTT ATAACATCAG CATCTTCTCT 
GTGGAAGAGA ATCAGGAGAG TGAACCTGTT TTTGTTCAGG TCACCACTGC TGGAGAGCCA 
CTGGCGGAGG AAGTGCCGTC TCCAACCGAT CTGCAGTTTT ATGAGGTGTC CGACTCAAAG 
ATCACTATCA CCTGGACTTC TCCGTCCTCT GAGGTGTCTG GATACCGTGT GTCTGTGGGT 
GAAGTGGGTC CAGATGGCTT GATTGAGAAA GAAGTGCCTT ATCCAGTAAC ACAGAATGCT 
TATGCTGAAA TCACACACCT TCAGCCCGGC ACCCTCTACC GCTTCTTCAT CTTTTCCCTC 
AAATCAGGAG AGGAGAGCGA ACCGCTGGTG GGAGAGCAAG CTACAAAGCC GGATCCCCCA 
ACTGACATCT ACTTCTCCAA CATTACTGAA GACAGTGCTG TGATCGTCTG GCTGCCTCCC 
ACTGCTCAGA TCACAGGTTA CAGGCTTTTC CTGACAGTCG AGGGCTCCAA CCCCAAACAG 
CTGCGTATTC CAGGCCGTCT GTCGCAGTAC ACCCTCCTCA ACCTGCAGCC GGACACAGAA 
TACTCTGCCA CTCTGCATGC AGAAAGAGGA AACGTGCTCA GTGAAGGCTC CATTGCTACC 
TTCGCCACAA CTCCTCCAAT GGGAAATGCT CCGTACTTTA GCACTGATGT CACAGACACT 
TCTATCATTG TCTCCTGGAG CCCTCTGCCC AAAATTGGAT ACAAGTTGAC AGTGAGGCCC 
AGCCAAGGTG GAGAAGCCCC TAGAGATGTG ACCTCAGAGT TGGGTAGTGT CCTCATCTCA 
GGTCTGACCC CTGGAGTTGA GTACACATAC AGTGTCCAAC CAGTCATCAG TGGTCAAGAG 
CATGGAAACC CTATAACTCG GCGCGTGGTC ACTCCTCTTT CTCCACCCAC TGATCTGAAC 
CTGGAGTCCA ACCCCAACAC CGGAAAGCTA ACTGTTCAGT GGAATGATGC TAACATCCCA 
GACATCACGG GCTACAGAGT GACCTGCACC CCCACCAAAG GCCAGCAGGG CAATTCACTT 
GAGGAGTTTG TGAAGGCCGG ACAGAACTCA TGCACATTAG AGAACCTGAG TCCAGGAGTA 
GAGTACAATG TCAGCGTGTT CACTGTGAAG GACGACATGG AGAGCGTTCC TGTCTTCACA 
ACTGTAACCC CAGATGTGCC TAAAATAACT GATCTCAGCT TCATCAATGT CACTGACTCT 
ACTATTGGCC TAAGCTGGAG TCCTCTAAAC TCCACTGCCG TTACCGGCTA CCGCATCACA 
GTTTTGGCAG CTGGGGACAG TGTCCCGATC TTTGTTGAAT TTGTGGAGCC CACAACAGGC 
TTTTATACTG TTCATGGCCT GGAGCCAGGC ATAGATTATG ACATCACCGT CACCACAGTT 
ACCGAAAATG GAGAGAGCGA ACCCATCACT ATCACCCAGC AAACAGCTGT CCCGGCTCCA 
TATGGCCTGT CATTTGGAGA AGTGACCGCA GACACTATGT TGGTGACCTG GAAAGCCCCT 
CAGGTTCCCA AATCCTCAGA CATTAATCAA TACATCATCC GTTATCATCC AGTCGATGAA 
GATGACGAAA CTACTGAACG CACTGTGGAA GGAAGTGAAA ACTTTGTTGT CCTTCGCCAT 
CTTGTGCCCA ACACAGAGTA CCTGGTGAGT GTGATTTGCG TATATGAGGG CAGAGAGGGA 
TCACCTGCCA TCGGCACCCA GAGAACTGTT TTTGATGCCC CTGTTGGCCT TCAGTTCTCT 
GATGTGGGCA CCACCTCCTT CACTGTGCGC TGGCAGGCTC CTCGGGCCAT CATCTCAGGA 
TACCGTATCC GCTACCAGAT GACCAGTGGT GGACGAGCTA AAGAGGAGAG ACTGCCCCCC 
TCGAGGAGCC ACTTCACCCT GACAGGCCTG ACCCCAGAGA CTGAGTACAG CATCAGTGTC 
TATGCCGTCA GCGGATCCAG AGACAGTCTG CCTCTGACTG GAACTCAGTC TACCATCTCT 
GATGCTCCTA CTGACCTGGA AGTGATTTCC TCAACGCCGA CCAGCATCAC CGTTCGCTGG 
GATGCCCCCT CTGTCACTGT GCGGTACTAC AGGATTACAC ATGGAGAGTC GGGTGGTTCT 
GATGCCCCTC TGGAATTTAT GGTTCCCGGC TCCCAGTCTA CAGCCACTAT TGAAGATCTA 
CGTCCAGGCA CTGATTATAC CATTACTGTT TATGCTGTGA CCGGTAGAGG GGACAGCCCT 
GCATCCAGCA CTCCCATCCA TGTTAAACAT AGAACCGATA TTGACTCTCC ATCACAGATG 
GAGGTGACTG ACGTGAAGGA CAACACTATT ACTGTACGCT GGACCCCAGC AGCCGGCCCC 
ATCTCAAGAT ACAGAGTCAA TGCAACTCCT CTTACTGGAG AAGGACCTGT GTTACATGCA 
GAAACGACTT CAGACCATAC TGAAATTACG TTTTCTGGCC TGATGCCTGC AGTGGAGTAC 
AGCATCAAGG TGTATGCTCT GGGTCAGGAT GGAGAGAGCC TCCCTCTGGT CGAAAATGTA 
GTTACAATGG ACAAGCCCAA GGATCTGTCC TTCACTGACG TGGAATCCAG CTCTATGCGC 
ATCTCCTGGG ACAGTCCCGA TGGCGTGGTC TCCTCCTACA GAGTGTTGTA CTACAGTCCA 
GAGGAGGGAG AGAGAGAGCT GTTTCCTGCC CCGCATGGAG ATGACGAGTC TGCAGTACTT 
CATGGCCTCA GACCCGGCAC AGAATATACT GTTAAAGTCA TCGCACTGCA TGACCAATCA 
CCCAGCACTG CACTGGTCGG CACTCAGACA ACAGGCATTC CTGGACCCAC AAGACTGCAG 
TTTTCCCAGG TGGGCCCAAC ATCCTTCACA GTCTCCTGGT CTTCATCTGA CGCCAGTTTG 
ACAGGTTATC GTGTGGCTGT TTCACCCAAG AGCAAAAGTG GCCCTACTAA AGAAGAAAAC 
ATCACTCCTG ACTCCACTGA GTTCCACGCC ACTGGACTCA TGCCTGGCAC TGATTACGAA 
GTGGAGGTTT ATGGTGTGAA GAACTCTCTA ACCAGCCCTC GTGTCAAAGG AGAAATCACA 
ACACCTGACA ATATCAGTCC ACCTCGACGT GTCCGTATAT CCAATGTGAA AGACTCCTCC 
ATCACTCTGA CCTGGCGCTC AAAGACTGAG GCCATTTCTG GCTTCTTGGT GGAAGCAACT 
CCCACCATGG GTGGCCATAA TCCAATCCAG AGGACCATTG AACCTGATTC ACGCACCTAC 
ACAATTGCTG GTCTGGAGCC AGGAACTAAT TATAAGATCA ATATTTATAC TCTGAATGGA 
AACTCTCGTA GTGAGCCTTT CACCCTTACA GCTACTACAG CAAAACCAGT TATTAGCCCA 
CCTACAAACC TCCATTTTAC TTCTCTGGCT TCTACTTCAA TCTCCTTCAC CTGGGAGCCT 
CCACGCAGCA CTATTACTGG ATATTATGTG ACCTATGAGG AGGCTGGAGG CATTCCCAAA 
GAGCTGACCC CTCGACCCCA GGCTGCCAGA ACCTTCGCGT CCATCTCTGG GTTGATACCA 
GGCACTGAGT ACATTATCAA GATTGTTGCA TTAAATGGCG CTCAGAGAAG CACGCCATTG 
ATTGGAAAGG CAAGAACTCA ACTGGAAATT GAGCAGCTTC CCCCTCAGCT ACCTAATCCC 
AGCCGACCCC ATCTCGAAGT CTTGGATGTG CCCGAGGACA ATGACAACTT CAACAACCAC 
GTACATGTGC TGGGGCCAAA CAGACACAAC ACACTGGGGC AGCAGGGTCA ACACATCTAC 
ACCGAATATC AGAGCTTCAA CCTGGGCAAC AACGGCCAGC AGCCTCATCA TCCAAACCAC 
GGCGAGCCGC TAGTCTACAT CCCTCTGCCG GGAGCGGACG GCCAGAGAGT GCCTGTGGTG 
AAGGTCAACG AGGGTCAAGA AGCAGGTTTC CCCTTTGGCG GCCTTTTTAA TGAGACAAAC 
CTGCCCCAGG AATCTCAGAC TCAGACTACA ATTGTGTGGC AGCCTGTGCC ACACACGTCT 
GAGTATGTGG TTTGGTGCGA CCCCATTACT GAGATCAATG AGAAGAGCTT CCAGATGCGT 
CTGCCTGGTA CATCAACAAG TGCTACCCTG ATTGGCCTTA CCTCAGGTGC CTCCTATAAT 
GTTCTTGTGG AGTCTGTTAA TGGAGACCAA AAACAGAAAG TGCTGCAAGA CGTTGTGACA 
GTAGGAAATA GCGTTCCAGG AGCTGGAGTG ATTCCCACAG GCAGGGATGT GTGTTATGAT 
TCATTCACCG CCACCTATCA TGAGGTTAAC ACGGAGTGGG AACGCATGTC CGAGACAGGC 
TTTAAACTTT GGTGCAAGTG CCTGGGCCTT GGCAGTGGTC ATTTCAGATG TGATTCATCC 
AAATGGTGCC ATGACAGTGG CCACAACTAC CGAATCGGTG AGAAATGGGA CCGGCGTGCT 
GAGAACGGCC ACATGATGAG CTGTACATGT CTGGGAAATG GGAAAGGAGA GTTTAAGTGT 
GAACCTCATG AATCCACCTG TTATGACGAG GGGAAATTGT ACCAGGTGGG CAACCAGTGG 
CAAAAGGAGT ATCTTGGAGC CATTTGCACA TGTACCTGCT ACGGAGGACA ACAGGGCTGG 
CGCTGTGAGA ACTGTCGCCG TCCTGGTGCT GAAGTTGATG CAGACCTCAT TCAGCCTCCA 
GTACGCACTG ATGCTTTCGA CCGATACAGG GAGAACGCTC TACGCAAACT GAACATCCAG 
TGTCCCATTG AGTGCCTGAG ACCAGACCTG TTAGCAGATG CCCATAGCCC TCATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302778.1
CDS	184..7623
Translation	MFGGPLGTALAVALLACAVQCMPKGTGKHKRQAQEHSVSSVSQDGCVMDGQFYGAGEKWERIYLGSTLLCTCHGVSKIECKSKPDAEETCYDKVNSRSYRVGETYERPKDGMIWDCTCIGSARGKISCTIANRCHEGGHSYKIGDTWQRPHDTGDYMLECVCLGNGKGEWTCKPVAERCYDNTAGTSYMVGETWEKPYQGWMIVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKTDSRGHVLQCLCTGNGRGEWKCERHASLHTTSLGTGSRVVTNVQPAVYHPQGVPEHPVEGSCLTEAGVSYAPGMRWSKAQGSKQMLCTCLGNGVSCEESESQSQVYGGTSGGEPCAFPFVFMGKTFYSCTSEGRNDGQLWCSTSSDFEKDYKYSFCTSNNVVVTTRGGNSNGALCQFPFLYNGRNYTDCTADGRRDGMKWCGTTYNFDKEQRFGFCPMAAHEEVCTTNEGVMYRVGDQWDRRHDVLGHMMRCTCVGNGRGEWSCIAYSQLKDQCIVDNLTYEVNQTFTKRHDEGYTMNCTCFGQGRGRWKCDAIDQCQEPETRVFYQIGESWDKLIQGIHYRCYCYGNGIGELSCEPQHSISGGHRPVQVIISEAGNQPNSHPIQWNAPASAHITQYILKWRPKNTHIQWMEVTIPGHVNSYTIAGLKPGVTYEGQLISILRFGRRETTRFDFSTVHGSLETSEGETTQPPRMVDTSESVTEITSSSFVISWVSASDTVSGFRVEYELSEEGGQTGQPMILDLPHSATSVNISELLPGRKYTVNVYEVTGEGEPNLILTTSQTTAPDAPSDHEVEDVADTSIRISWSKPLAPITGYRVVYTPSEEGSSGSVELNLPEHSTSLTLGDLRPGVLYNISIFSVEENQESEPVFVQVTTAGEPLAEEVPSPTDLQFYEVSDSKITITWTSPSSEVSGYRVSVGEVGPDGLIEKEVPYPVTQNAYAEITHLQPGTLYRFFIFSLKSGEESEPLVGEQATKPDPPTDIYFSNITEDSAVIVWLPPTAQITGYRLFLTVEGSNPKQLRIPGRLSQYTLLNLQPDTEYSATLHAERGNVLSEGSIATFATTPPMGNAPYFSTDVTDTSIIVSWSPLPKIGYKLTVRPSQGGEAPRDVTSELGSVLISGLTPGVEYTYSVQPVISGQEHGNPITRRVVTPLSPPTDLNLESNPNTGKLTVQWNDANIPDITGYRVTCTPTKGQQGNSLEEFVKAGQNSCTLENLSPGVEYNVSVFTVKDDMESVPVFTTVTPDVPKITDLSFINVTDSTIGLSWSPLNSTAVTGYRITVLAAGDSVPIFVEFVEPTTGFYTVHGLEPGIDYDITVTTVTENGESEPITITQQTAVPAPYGLSFGEVTADTMLVTWKAPQVPKSSDINQYIIRYHPVDEDDETTERTVEGSENFVVLRHLVPNTEYLVSVICVYEGREGSPAIGTQRTVFDAPVGLQFSDVGTTSFTVRWQAPRAIISGYRIRYQMTSGGRAKEERLPPSRSHFTLTGLTPETEYSISVYAVSGSRDSLPLTGTQSTISDAPTDLEVISSTPTSITVRWDAPSVTVRYYRITHGESGGSDAPLEFMVPGSQSTATIEDLRPGTDYTITVYAVTGRGDSPASSTPIHVKHRTDIDSPSQMEVTDVKDNTITVRWTPAAGPISRYRVNATPLTGEGPVLHAETTSDHTEITFSGLMPAVEYSIKVYALGQDGESLPLVENVVTMDKPKDLSFTDVESSSMRISWDSPDGVVSSYRVLYYSPEEGERELFPAPHGDDESAVLHGLRPGTEYTVKVIALHDQSPSTALVGTQTTGIPGPTRLQFSQVGPTSFTVSWSSSDASLTGYRVAVSPKSKSGPTKEENITPDSTEFHATGLMPGTDYEVEVYGVKNSLTSPRVKGEITTPDNISPPRRVRISNVKDSSITLTWRSKTEAISGFLVEATPTMGGHNPIQRTIEPDSRTYTIAGLEPGTNYKINIYTLNGNSRSEPFTLTATTAKPVISPPTNLHFTSLASTSISFTWEPPRSTITGYYVTYEEAGGIPKELTPRPQAARTFASISGLIPGTEYIIKIVALNGAQRSTPLIGKARTQLEIEQLPPQLPNPSRPHLEVLDVPEDNDNFNNHVHVLGPNRHNTLGQQGQHIYTEYQSFNLGNNGQQPHHPNHGEPLVYIPLPGADGQRVPVVKVNEGQEAGFPFGGLFNETNLPQESQTQTTIVWQPVPHTSEYVVWCDPITEINEKSFQMRLPGTSTSATLIGLTSGASYNVLVESVNGDQKQKVLQDVVTVGNSVPGAGVIPTGRDVCYDSFTATYHEVNTEWERMSETGFKLWCKCLGLGSGHFRCDSSKWCHDSGHNYRIGEKWDRRAENGHMMSCTCLGNGKGEFKCEPHESTCYDEGKLYQVGNQWQKEYLGAICTCTCYGGQQGWRCENCRRPGAEVDADLIQPPVRTDAFDRYRENALRKLNIQCPIECLRPDLLADAHSPHD

Target ORF information:

RefSeq Version	XM_009304503.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio fibronectin 1a (fn1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304503.2

ORF Insert Sequence:

ATGTTTGGTG GCCCTTTGGG CACGGCGCTT GCGGTGGCAT TGCTTGCCTG CGCTGTGCAG 
TGTATGCCGA AAGGAACGGG GAAACATAAA AGACAAGCTC AGGAACACTC TGTATCATCT 
GTGTCACAAG ATGGATGTGT TATGGACGGA CAGTTTTATG GAGCTGGAGA GAAGTGGGAG 
CGCATTTATC TGGGCAGCAC GCTTCTTTGC ACTTGTCATG GAGTTTCTAA AATAGAGTGT 
AAATCAAAAC CTGATGCTGA GGAGACCTGC TACGATAAAG TCAATTCTCG TTCCTACCGG 
GTTGGAGAGA CTTACGAGAG GCCCAAGGAC GGAATGATCT GGGACTGTAC TTGCATTGGC 
TCTGCCCGAG GGAAAATCAG TTGCACCATT GCGAACCGCT GTCATGAGGG AGGGCACTCC 
TACAAGATTG GAGACACATG GCAGAGACCT CATGACACTG GAGACTACAT GCTGGAGTGT 
GTCTGCTTGG GAAATGGGAA GGGGGAGTGG ACCTGTAAAC CTGTTGCGGA GCGCTGTTAT 
GATAATACAG CTGGCACCTC CTACATGGTT GGTGAGACCT GGGAGAAGCC CTACCAGGGC 
TGGATGATTG TGGACTGCAC CTGCCTCGGA GAAGGAAGTG GACGTATTAC ATGCACATCC 
AGAAACCGCT GTAATGACCA GGACACACGC ACTTCTTACC GGATTGGGGA CACGTGGAGT 
AAGACGGACT CTCGTGGACA CGTTTTACAG TGTTTGTGTA CTGGTAATGG ACGTGGAGAG 
TGGAAGTGTG AGAGGCATGC TTCTCTGCAC ACTACTAGTC TTGGTACTGG GTCTCGTGTT 
GTGACCAATG TCCAGCCAGC AGTGTACCAC CCACAAGGGG TGCCTGAACA CCCTGTGGAG 
GGATCCTGTC TGACTGAAGC AGGTGTTTCC TATGCTCCAG GCATGCGCTG GAGCAAGGCT 
CAAGGTAGCA AGCAGATGTT GTGTACCTGC TTAGGAAACG GTGTCAGCTG TGAAGAGTCA 
GAGAGTCAAT CTCAGGTGTA TGGCGGGACC TCAGGTGGCG AACCGTGTGC TTTCCCTTTT 
GTGTTCATGG GGAAAACGTT TTACTCCTGC ACGTCTGAGG GACGCAATGA TGGACAGCTT 
TGGTGCAGCA CCTCATCTGA CTTTGAGAAA GACTACAAGT ATTCCTTCTG TACCAGCAAC 
AATGTTGTGG TCACAACCAG AGGAGGAAAC TCTAATGGTG CCCTGTGCCA GTTCCCTTTC 
CTCTACAATG GCCGTAACTA CACTGACTGC ACTGCTGATG GCAGAAGAGA TGGCATGAAA 
TGGTGCGGCA CGACTTATAA CTTTGACAAA GAGCAGCGCT TTGGCTTCTG TCCAATGGCA 
GCTCATGAAG AGGTGTGTAC CACCAATGAG GGTGTGATGT ATCGTGTGGG GGATCAATGG 
GACAGACGCC ATGATGTTCT GGGTCACATG ATGCGCTGTA CGTGTGTGGG CAATGGCCGT 
GGAGAATGGA GCTGCATCGC CTACTCTCAA CTCAAAGACC AGTGCATAGT GGATAATTTG 
ACCTATGAAG TAAATCAGAC TTTTACTAAA AGGCACGACG AAGGCTACAC AATGAACTGC 
ACCTGTTTCG GTCAAGGTCG TGGACGCTGG AAGTGTGATG CCATCGATCA GTGCCAGGAG 
CCGGAGACTA GAGTTTTCTA TCAGATTGGA GAGTCCTGGG ACAAACTCAT TCAGGGAATC 
CACTACAGAT GTTACTGCTA TGGTAATGGC ATCGGGGAGT TGAGCTGCGA GCCTCAGCAT 
TCTATCTCTG GTGGTCATCG TCCAGTCCAG GTCATTATTT CAGAAGCTGG AAATCAGCCT 
AACTCGCACC CCATCCAATG GAATGCACCA GCTTCTGCTC ACATCACACA GTACATACTT 
AAATGGAGGC CGAAAAACAC CCATATTCAG TGGATGGAGG TGACTATTCC AGGCCATGTC 
AACTCTTACA CTATTGCGGG ACTGAAGCCG GGAGTCACCT ACGAGGGTCA GCTCATCAGT 
ATCCTGCGTT TTGGACGTAG AGAGACCACT CGCTTTGATT TTTCCACCGT ACATGGCTCA 
TTGGAGACCT CTGAGGGTGA GACCACCCAG CCCCCTCGAA TGGTGGACAC CTCTGAGTCA 
GTGACTGAAA TCACCTCAAG CAGTTTCGTC ATCTCCTGGG TGTCTGCGTC TGACACCGTG 
TCTGGTTTTA GGGTGGAATA TGAGCTGAGT GAAGAGGGAG GCCAGACTGG ACAGCCCATG 
ATCTTGGACC TCCCTCATAG CGCTACGTCC GTGAACATCA GTGAACTTCT GCCAGGAAGG 
AAGTACACTG TGAATGTATA TGAAGTGACA GGAGAAGGAG AACCTAACCT CATCCTCACC 
ACTTCCCAGA CTACTGCTCC TGATGCTCCT TCTGATCATG AAGTGGAGGA TGTAGCAGAC 
ACCTCCATTC GTATCAGCTG GTCTAAACCT TTGGCTCCCA TCACAGGTTA CCGTGTAGTT 
TACACCCCAT CTGAAGAGGG CAGTTCTGGC AGTGTTGAGC TGAATCTGCC AGAACATTCC 
ACCTCATTGA CCCTTGGTGA TCTTCGACCT GGTGTGCTTT ATAACATCAG CATCTTCTCT 
GTGGAAGAGA ATCAGGAGAG TGAACCTGTT TTTGTTCAGG TCACCACTGC TGGAGAGCCA 
CTGGCGGAGG AAGTGCCGTC TCCAACCGAT CTGCAGTTTT ATGAGGTGTC CGACTCAAAG 
ATCACTATCA CCTGGACTTC TCCGTCCTCT GAGGTGTCTG GATACCGTGT GTCTGTGGGT 
GAAGTGGGTC CAGATGGCTT GATTGAGAAA GAAGTGCCTT ATCCAGTAAC ACAGAATGCT 
TATGCTGAAA TCACACACCT TCAGCCCGGC ACCCTCTACC GCTTCTTCAT CTTTTCCCTC 
AAATCAGGAG AGGAGAGCGA ACCGCTGGTG GGAGAGCAAG CTACAAAGCC GGATCCCCCA 
ACTGACATCT ACTTCTCCAA CATTACTGAA GACAGTGCTG TGATCGTCTG GCTGCCTCCC 
ACTGCTCAGA TCACAGGTTA CAGGCTTTTC CTGACAGTCG AGGGCTCCAA CCCCAAACAG 
CTGCGTATTC CAGGCCGTCT GTCGCAGTAC ACCCTCCTCA ACCTGCAGCC GGACACAGAA 
TACTCTGCCA CTCTGCATGC AGAAAGAGGA AACGTGCTCA GTGAAGGCTC CATTGCTACC 
TTCGCCACAA CTCCTCCAAT GGGAAATGCT CCGTACTTTA GCACTGATGT CACAGACACT 
TCTATCATTG TCTCCTGGAG CCCTCTGCCC AAAATTGGAT ACAAGTTGAC AGTGAGGCCC 
AGCCAAGGTG GAGAAGCCCC TAGAGATGTG ACCTCAGAGT TGGGTAGTGT CCTCATCTCA 
GGTCTGACCC CTGGAGTTGA GTACACATAC AGTGTCCAAC CAGTCATCAG TGGTCAAGAG 
CATGGAAACC CTATAACTCG GCGCGTGGTC ACTCCTCTTT CTCCACCCAC TGATCTGAAC 
CTGGAGTCCA ACCCCAACAC CGGAAAGCTA ACTGTTCAGT GGAATGATGC TAACATCCCA 
GACATCACGG GCTACAGAGT GACCTGCACC CCCACCAAAG GCCAGCAGGG CAATTCACTT 
GAGGAGTTTG TGAAGGCCGG ACAGAACTCA TGCACATTAG AGAACCTGAG TCCAGGAGTA 
GAGTACAATG TCAGCGTGTT CACTGTGAAG GACGACATGG AGAGCGTTCC TGTCTTCACA 
ACTGTAACCC CAGATGTGCC TAAAATAACT GATCTCAGCT TCATCAATGT CACTGACTCT 
ACTATTGGCC TAAGCTGGAG TCCTCTAAAC TCCACTGCCG TTACCGGCTA CCGCATCACA 
GTTTTGGCAG CTGGGGACAG TGTCCCGATC TTTGTTGAAT TTGTGGAGCC CACAACAGGC 
TTTTATACTG TTCATGGCCT GGAGCCAGGC ATAGATTATG ACATCACCGT CACCACAGTT 
ACCGAAAATG GAGAGAGCGA ACCCATCACT ATCACCCAGC AAACAGCTGT CCCGGCTCCA 
TATGGCCTGT CATTTGGAGA AGTGACCGCA GACACTATGT TGGTGACCTG GAAAGCCCCT 
CAGGTTCCCA AATCCTCAGA CATTAATCAA TACATCATCC GTTATCATCC AGTCGATGAA 
GATGACGAAA CTACTGAACG CACTGTGGAA GGAAGTGAAA ACTTTGTTGT CCTTCGCCAT 
CTTGTGCCCA ACACAGAGTA CCTGGTGAGT GTGATTTGCG TATATGAGGG CAGAGAGGGA 
TCACCTGCCA TCGGCACCCA GAGAACTGTT TTTGATGCCC CTGTTGGCCT TCAGTTCTCT 
GATGTGGGCA CCACCTCCTT CACTGTGCGC TGGCAGGCTC CTCGGGCCAT CATCTCAGGA 
TACCGTATCC GCTACCAGAT GACCAGTGGT GGACGAGCTA AAGAGGAGAG ACTGCCCCCC 
TCGAGGAGCC ACTTCACCCT GACAGGCCTG ACCCCAGAGA CTGAGTACAG CATCAGTGTC 
TATGCCGTCA GCGGATCCAG AGACAGTCTG CCTCTGACTG GAACTCAGTC TACCATCTCT 
GATGCTCCTA CTGACCTGGA AGTGATTTCC TCAACGCCGA CCAGCATCAC CGTTCGCTGG 
GATGCCCCCT CTGTCACTGT GCGGTACTAC AGGATTACAC ATGGAGAGTC GGGTGGTTCT 
GATGCCCCTC TGGAATTTAT GGTTCCCGGC TCCCAGTCTA CAGCCACTAT TGAAGATCTA 
CGTCCAGGCA CTGATTATAC CATTACTGTT TATGCTGTGA CCGGTAGAGG GGACAGCCCT 
GCATCCAGCA CTCCCATCCA TGTTAAACAT AGAACCGATA TTGACTCTCC ATCACAGATG 
GAGGTGACTG ACGTGAAGGA CAACACTATT ACTGTACGCT GGACCCCAGC AGCCGGCCCC 
ATCTCAAGAT ACAGAGTCAA TGCAACTCCT CTTACTGGAG AAGGACCTGT GTTACATGCA 
GAAACGACTT CAGACCATAC TGAAATTACG TTTTCTGGCC TGATGCCTGC AGTGGAGTAC 
AGCATCAAGG TGTATGCTCT GGGTCAGGAT GGAGAGAGCC TCCCTCTGGT CGAAAATGTA 
GTTACAATGG ACAAGCCCAA GGATCTGTCC TTCACTGACG TGGAATCCAG CTCTATGCGC 
ATCTCCTGGG ACAGTCCCGA TGGCGTGGTC TCCTCCTACA GAGTGTTGTA CTACAGTCCA 
GAGGAGGGAG AGAGAGAGCT GTTTCCTGCC CCGCATGGAG ATGACGAGTC TGCAGTACTT 
CATGGCCTCA GACCCGGCAC AGAATATACT GTTAAAGTCA TCGCACTGCA TGACCAATCA 
CCCAGCACTG CACTGGTCGG CACTCAGACA ACAGGCATTC CTGGACCCAC AAGACTGCAG 
TTTTCCCAGG TGGGCCCAAC ATCCTTCACA GTCTCCTGGT CTTCATCTGA CGCCAGTTTG 
ACAGGTTATC GTGTGGCTGT TTCACCCAAG AGCAAAAGTG GCCCTACTAA AGAAGAAAAC 
ATCACTCCTG ACTCCACTGA GTTCCACGCC ACTGGACTCA TGCCTGGCAC TGATTACGAA 
GTGGAGGTTT ATGGTGTGAA GAACTCTCTA ACCAGCCCTC GTGTCAAAGG AGAAATCACA 
ACACCTGACA ATATCAGTCC ACCTCGACGT GTCCGTATAT CCAATGTGAA AGACTCCTCC 
ATCACTCTGA CCTGGCGCTC AAAGACTGAG GCCATTTCTG GCTTCTTGGT GGAAGCAACT 
CCCACCATGG GTGGCCATAA TCCAATCCAG AGGACCATTG AACCTGATTC ACGCACCTAC 
ACAATTGCTG GTCTGGAGCC AGGAACTAAT TATAAGATCA ATATTTATAC TCTGAATGGA 
AACTCTCGTA GTGAGCCTTT CACCCTTACA GCTACTACAG CAAAACCAGT TATTAGCCCA 
CCTACAAACC TCCATTTTAC TTCTCTGGCT TCTACTTCAA TCTCCTTCAC CTGGGAGCCT 
CCACGCAGCA CTATTACTGG ATATTATGTG ACCTATGAGG AGGCTGGAGG CATTCCCAAA 
GAGCTGACCC CTCGACCCCA GGCTGCCAGA ACCTTCGCGT CCATCTCTGG GTTGATACCA 
GGCACTGAGT ACATTATCAA GATTGTTGCA TTAAATGGCG CTCAGAGAAG CACGCCATTG 
ATTGGAAAGG CAAGAACTCA ACTGGAAATT GAGCAGCTTC CCCCTCAGCT ACCTAATCCC 
AGCCGACCCC ATCTCGAAGT CTTGGATGTG CCCGAGGACA ATGACAACTT CAACAACCAC 
GTACATGTGC TGGGGCCAAA CAGACACAAC ACACTGGGGC AGCAGGGTCA ACACATCTAC 
ACCGAATATC AGAGCTTCAA CCTGGGCAAC AACGGCCAGC AGCCTCATCA TCCAAACCAC 
GGCGAGCCGC TAGTCTACAT CCCTCTGCCG GGAGCGGACG GCCAGAGAGT GCCTGTGGTG 
AAGGTCAACG AGGGTCAAGA AGCAGGTTTC CCCTTTGGCG GCCTTTTTAA TGAGACAAAC 
CTGCCCCAGG AATCTCAGAC TCAGACTACA ATTGTGTGGC AGCCTGTGCC ACACACGTCT 
GAGTATGTGG TTTGGTGCGA CCCCATTACT GAGATCAATG AGAAGAGCTT CCAGATGCGT 
CTGCCTGGTA CATCAACAAG TGCTACCCTG ATTGGCCTTA CCTCAGGTGC CTCCTATAAT 
GTTCTTGTGG AGTCTGTTAA TGGAGACCAA AAACAGAAAG TGCTGCAAGA CGTTGTGACA 
GTAGGAAATA GCGTTCCAGG AGCTGGAGTG ATTCCCACAG GCAGGGATGT GTGTTATGAT 
TCATTCACCG CCACCTATCA TGAGGTTAAC ACGGAGTGGG AACGCATGTC CGAGACAGGC 
TTTAAACTTT GGTGCAAGTG CCTGGGCCTT GGCAGTGGTC ATTTCAGATG TGATTCATCC 
AAATGGTGCC ATGACAGTGG CCACAACTAC CGAATCGGTG AGAAATGGGA CCGGCGTGCT 
GAGAACGGCC ACATGATGAG CTGTACATGT CTGGGAAATG GGAAAGGAGA GTTTAAGTGT 
GAACCTCATG AATCCACCTG TTATGACGAG GGGAAATTGT ACCAGGTGGG CAACCAGTGG 
CAAAAGGAGT ATCTTGGAGC CATTTGCACA TGTACCTGCT ACGGAGGACA ACAGGGCTGG 
CGCTGTGAGA ACTGTCGCCG TCCTGGTGCT GAAGTTGATG CAGACCTCAT TCAGCCTCCA 
GTACGCACTG ATGCTTTCGA CCGATACAGG GAGAACGCTC TACGCAAACT GAACATCCAG 
TGTCCCATTG AGTGCCTGAG ACCAGACCTG TTAGCAGATG CCCATAGCCC TCATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Cell reports27(5)1567-1578.e5(2019 04)
Xue Y,Liu D,Cui G,Ding Y,Ai D,Gao S,Zhang Y,Suo S,Wang X,Lv P,Zhou C,Li Y,Chen X,Peng G,Jing N,Han JJ,Liu F

The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling.
eLife7(2018 12)
Rasouli SJ,El-Brolosy M,Tsedeke AT,Bensimon-Brito A,Ghanbari P,Maischein HM,Kuenne C,Stainier DY

Vangl2-dependent regulation of membrane protrusions and directed migration requires a fibronectin extracellular matrix.
Development (Cambridge, England)145(22)(2018 11)
Love AM,Prince DJ,Jessen JR

Glypican 4 and Mmp14 interact in regulating the migration of anterior endodermal cells by limiting extracellular matrix deposition.
Development (Cambridge, England)145(17)(2018 08)
Hu B,Gao Y,Davies L,Woo S,Topczewski J,Jessen JR,Lin F

Transient fibrosis resolves via fibroblast inactivation in the regenerating zebrafish heart.
Proceedings of the National Academy of Sciences of the United States of America115(16)4188-4193(2018 04)
Sánchez-zo H,Galardi-Castilla M,Sanz-Morejón A,González-Rosa JM,Costa R,Ernst A,Sainz de Aja J,Langa X,Mercader N

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FN1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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