iars2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	iars2
Entrez Gene ID	100001219
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
Synonyms	si:ch211-214p16.3,si:zc214p16.3
General protein information	Preferred Names isoleucyl-tRNA synthetase 2, mitochondrial Names isoleucine--tRNA ligase, mitochondrial isoleucine tRNA synthetase isoleucine-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163798.1	NP_001157270.1	isoleucine--tRNA ligase, mitochondrial
XM_021467083.1	XP_021322758.1	isoleucine--tRNA ligase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Neurospora crassa	NCU00920	XP_964746.2
Eremothecium gossypii	AGOS_AGL247C	NP_986420.1
Drosophila melanogaster (fruit fly)	CG5414	NP_648837.2
Arabidopsis thaliana (thale cress)	OVA2	NP_199714.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003156	XP_312846.5
Canis lupus familiaris (dog)	IARS2	XP_536121.3
Gallus gallus (chicken)	IARS2	NP_001006397.1
Saccharomyces cerevisiae (baker's yeast)	ISM1	NP_015285.1
Schizosaccharomyces pombe (fission yeast)	SPCC18B5.08c	NP_587938.1
Kluyveromyces lactis	KLLA0D11858g	XP_453590.1
Homo sapiens (human)	IARS2	NP_060530.3
Pan troglodytes (chimpanzee)	IARS2	XP_514209.2
Macaca mulatta (Rhesus monkey)	IARS2	XP_001099877.1
Caenorhabditis elegans (roundworm)	iars-2	NP_001251317.1
Danio rerio (zebrafish)	iars2	NP_001157270.1
Xenopus tropicalis (tropical clawed frog)	iars2	NP_001120515.1
Bos taurus (cattle)	IARS2	NP_001193724.1
Mus musculus (house mouse)	Iars2	NP_941055.1
Rattus norvegicus (Norway rat)	Iars2	XP_006250494.1
Magnaporthe oryzae (rice blast fungus)	MGG_01165	XP_003714047.1
Oryza sativa (rice)	Os02g0778200	NP_001048293.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Reconstruction of a 450-My-old ancestral vertebrate protokaryotype.
Kohn M, Högel J, Vogel W, Minich P, Kehrer-Sawatzki H, Graves JA, Hameister H
Trends in genetics : TIG22(4)203-10(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following iars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the iars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03244	NM_001163798.1 Latest version!	Danio rerio isoleucyl-tRNA synthetase 2, mitochondrial (iars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa149498	XM_021467083.1 Latest version!	Danio rerio isoleucyl-tRNA synthetase 2, mitochondrial (iars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

iars2 ( NM_001163798.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001157270.1 Danio rerio isoleucyl-tRNA synthetase 2, mitochondrial (iars2), mRNA.

Price & Availability↑

CloneID	ODa03244
Clone ID Related Accession (Same CDS sequence)	NM_001163798.1
Accession Version	NM_001163798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	isoleucine--tRNA ligase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FP015823.9 and AL929207.5. On Aug 1, 2009 this sequence version replaced XM_001335330.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01013694.1, GFIL01013055.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTGCTGG TTCGGCGTTC GGCAGCAGGT TTCGGCGGCG TGGTGCGATG GGGACTGCGC 
TGTCTGTGCT CCGGGGACGC GGCGGGCCGG TACCGAAACA CCGTTCTGCT GCCCCGCACT 
GACTTCCCTA TAAAAGTGAA TGGGCCAAGC CTTCTAGAGC AAGAGATAAA GATCCAGCAG 
AAATGTGGTT TTGACCAGCT GTACAAATGG CAGAGAGAGA AAAAGGCTAA GAAGGAGTAC 
TGTCTTCATG ATGGACCACC GTATGCCAAT GGGGACCCTC ATGTTGGGCA TGCACTCAAT 
AAGATTCTCA AAGACATACG AAACCGTTTC GAGGTGCTGA GAGGCAGGCA GGTGCACTAT 
GTGCCCGGCT GGGACTGTCA TGGTCTGCCC ATTGAACTCA AAGCGCTGGG AGACCTGGGA 
ACCAATGAGC TGACTCCTGT CCAGATCAGA CAGAAAGCTC GTGAGTTTGC AGAGAAGGCC 
ATATCTCGAC AGCGGGCTGC TTTCCAGCGC TGGGGTGTGA TGGCTGACTG GGACAACTGT 
TATTACACTT TTGATGGGAA ATATGAAGCA GCCCAGCTCA AGGTCTTCCA GGAGATGCAC 
AACAAGGGTT TGATTTATCA AGACTATAAG CCTGTTTTCT GGTCACCGTC GTCCAGGACG 
GCGCTTGCAG AAGCTGAGCT GGAGTATAAT CCAGAACATG TGAGCCGTTC TCTGTACATC 
ACTTTCCCCC TGCTCACCCT GCCAGCTAAA CTCACAGCAA AAGCAGAAGG TTTGAGTAAT 
GTGTCTGCGC TGATATGGAC AACGCAACCA TGGACTATTC CAGCCAATCA GGCAGTTTGT 
TATATGCCTA AAGTACAGTA TTCTGTAGTG AAGCGAGCTG ATACTGAGCA GCTCCTGCTA 
GTGGCCACAG AGCGAATAAA CAGCCTCTCC TCTTTACTGA AGACAAATCT GGAGAGTTTG 
GTCACATTTG CAGGGACAGA TCTTGAAGGC GGAGTCTGTC AGCATCCCAC AATTCCTTCA 
AAAGAAGTAC CTTTGCTGCC AGCTAGTCAT GTCACCATGA CTAAGGGAAC AGGATTGGTC 
CATACAGCTC CAGCCCATGG CATGGATGAC TACAGTGTCG CCACACACTT CAATCTACCA 
GTAGAGTGCA TGGTGGATGA AGAGGGCAGG TTTACTGAAC TTGCTGGAGC TGAACTGCAG 
AATAAATCTG TGATGGACGA AGGCAATGCT GCAGTAATCT CAGTGCTGCA GGCGGCAGGT 
GCAGTAGTGA AGGAGGAGGA CTGTGTCCAC AGTTACCCGT ATGACTGGAG GACCAAACAG 
CCAGTCGTGA TCCGAGCCAG TAGACAGTGG TTTATTAATA CTGCCACCCT CAAAGATAAA 
GCAAAGGATG CCCTGCAGAA AGTGCGTGTG ATACCCGAAT CTGCGAGGTC CAGTTTGTTG 
TCAATGTTGG ACAGAAGAAC ATACTGGTGT ATATCTCGAC AGAGGAGCTG GGGCGTCCCT 
ATCCCAGTGT TCTACCATAA AGAGACTGGA GAGCCACTCT TAAACAAACA TACAGTGACC 
CACATCGCTA AAGTTTTCTC TGAGAAGGGG AGCGACAGCT GGTGGACAGA GCCAGTGGAG 
ACGTTTTTGA CACCAGATGT TCTTCAGAAG AGTAAAGCAA GTTCAGTGTC TGATTATGTG 
CGGGGTGAAG ATGTCCTGGA TATCTGGTTT GACAGTGGGG CTTCATGGGC CGCTGTACTG 
CCAGAATCAG ACCCGCGTGC AGACTCGTAT GTCGAGGGGA AAGATCAGAT CGGCGGCTGG 
TTTCAGTCGT CTCTCCTCAC CAGTGTTGCT GTGCGGAATA AAGCTCCTTA CAAAGCTCTA 
GTTGTTCATG GCTTTGCCGT GAGTGAGAAA GGAGAGAAAA TGTCTAAGTC CATAGGCAAC 
GTGGTGGACC CAGATGTGGT TATTAATGGG GGGAAGGACT CGTCTGTTTC TCCTCCATAT 
GGTGCTGATG TTTTGAGATG GTGGGTTGCA GAGTCCAATG TCTTTTCTGA GGTTCAGATC 
GGACCAAATG CACTGAACGC AGCTAAAGAC AGCATCAACA AGCTGAGGAA CACACTGAAG 
TTTCTCTTAG GCAATCTCCA GGGCTTTGAT CCTCGTTCTC AGGCCGTGGA CCCCAAACAA 
ATGCACTACA TTGACCAGTA CATGCTGCAT CTGCTCCGAG AGTTCAGCAT GAAGGTAACC 
GATGCTTACA GTGAGTTCAA CTATGGCCTT GTCATCCGTC TGCTGCAATC CTTCACCGCT 
AGAGAATTAT CCAACTTCTA TTTCAGCATC ATCAAAGATC GGCTATATTG TGACCCAGAG 
GACTCTGTTG GTCGGCGGTC GTGTCAGACA GTCCTGGAGG AGATTCTGGA TGGAGTGAGT 
CGCTCTATCG CTCCAGTCCT CCCTCATCTA GCTGAGGAGG TGTACCTGCA CGCTCCTGGA 
CATGATGAGG GGAACACTTT GTTTCGGAGC GGCTGGATTA ACACCAGTCC TGTGTGGAGA 
AAACCGGGAC TGGAGGAAGC AGTGGAGGGC GCGTGTGCTA TTAGAGACTC CTTCCTGTCA 
TCTATTCCAG GACGAAATGC TGCCGAGTTT GACCTCGTCA TTTCCATTGA ACCCGGCCTG 
TTCTTTGAGC TCATGGAGTC TCTTCAAGAG GAGCCCACTT CCACCAGCTC TCAGCTGACA 
GAGCTCATGA TGACCTCGCG CACCACTCTG ACAAGCGCTC TCCCACGAGA CCTGCCATCC 
GATGCCATCA CAAGCACGGG GAGCTTCCTC ATCAACCTGG AAGGAGGCAT GATCCGTGAG 
GAAAGCTCAT ACAGCATAGC AGCGATGCCC ACCGCTCTGT CGCGATGCCC GAGATGCCGA 
CGCTACACAT CCGATGAGCC AGACTGCCTC TGTCCGCGAT GCCAAACTAT GATGGAAAAA 
AATGGCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157270.1
CDS	110..3061
Translation	MLLVRRSAAGFGGVVRWGLRCLCSGDAAGRYRNTVLLPRTDFPIKVNGPSLLEQEIKIQQKCGFDQLYKWQREKKAKKEYCLHDGPPYANGDPHVGHALNKILKDIRNRFEVLRGRQVHYVPGWDCHGLPIELKALGDLGTNELTPVQIRQKAREFAEKAISRQRAAFQRWGVMADWDNCYYTFDGKYEAAQLKVFQEMHNKGLIYQDYKPVFWSPSSRTALAEAELEYNPEHVSRSLYITFPLLTLPAKLTAKAEGLSNVSALIWTTQPWTIPANQAVCYMPKVQYSVVKRADTEQLLLVATERINSLSSLLKTNLESLVTFAGTDLEGGVCQHPTIPSKEVPLLPASHVTMTKGTGLVHTAPAHGMDDYSVATHFNLPVECMVDEEGRFTELAGAELQNKSVMDEGNAAVISVLQAAGAVVKEEDCVHSYPYDWRTKQPVVIRASRQWFINTATLKDKAKDALQKVRVIPESARSSLLSMLDRRTYWCISRQRSWGVPIPVFYHKETGEPLLNKHTVTHIAKVFSEKGSDSWWTEPVETFLTPDVLQKSKASSVSDYVRGEDVLDIWFDSGASWAAVLPESDPRADSYVEGKDQIGGWFQSSLLTSVAVRNKAPYKALVVHGFAVSEKGEKMSKSIGNVVDPDVVINGGKDSSVSPPYGADVLRWWVAESNVFSEVQIGPNALNAAKDSINKLRNTLKFLLGNLQGFDPRSQAVDPKQMHYIDQYMLHLLREFSMKVTDAYSEFNYGLVIRLLQSFTARELSNFYFSIIKDRLYCDPEDSVGRRSCQTVLEEILDGVSRSIAPVLPHLAEEVYLHAPGHDEGNTLFRSGWINTSPVWRKPGLEEAVEGACAIRDSFLSSIPGRNAAEFDLVISIEPGLFFELMESLQEEPTSTSSQLTELMMTSRTTLTSALPRDLPSDAITSTGSFLINLEGGMIREESSYSIAAMPTALSRCPRCRRYTSDEPDCLCPRCQTMMEKNGK

Target ORF information:

RefSeq Version	NM_001163798.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio isoleucyl-tRNA synthetase 2, mitochondrial (iars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163798.1

ORF Insert Sequence:

ATGCTGCTGG TTCGGCGTTC GGCAGCAGGT TTCGGCGGCG TGGTGCGATG GGGACTGCGC 
TGTCTGTGCT CCGGGGACGC GGCGGGCCGG TACCGAAACA CCGTTCTGCT GCCCCGCACT 
GACTTCCCTA TAAAAGTGAA TGGGCCAAGC CTTCTAGAGC AAGAGATAAA GATCCAGCAG 
AAATGTGGTT TTGACCAGCT GTACAAATGG CAGAGAGAGA AAAAGGCTAA GAAGGAGTAC 
TGTCTTCATG ATGGACCACC GTATGCCAAT GGGGACCCTC ATGTTGGGCA TGCACTCAAT 
AAGATTCTCA AAGACATACG AAACCGTTTC GAGGTGCTGA GAGGCAGGCA GGTGCACTAT 
GTGCCCGGCT GGGACTGTCA TGGTCTGCCC ATTGAACTCA AAGCGCTGGG AGACCTGGGA 
ACCAATGAGC TGACTCCTGT CCAGATCAGA CAGAAAGCTC GTGAGTTTGC AGAGAAGGCC 
ATATCTCGAC AGCGGGCTGC TTTCCAGCGC TGGGGTGTGA TGGCTGACTG GGACAACTGT 
TATTACACTT TTGATGGGAA ATATGAAGCA GCCCAGCTCA AGGTCTTCCA GGAGATGCAC 
AACAAGGGTT TGATTTATCA AGACTATAAG CCTGTTTTCT GGTCACCGTC GTCCAGGACG 
GCGCTTGCAG AAGCTGAGCT GGAGTATAAT CCAGAACATG TGAGCCGTTC TCTGTACATC 
ACTTTCCCCC TGCTCACCCT GCCAGCTAAA CTCACAGCAA AAGCAGAAGG TTTGAGTAAT 
GTGTCTGCGC TGATATGGAC AACGCAACCA TGGACTATTC CAGCCAATCA GGCAGTTTGT 
TATATGCCTA AAGTACAGTA TTCTGTAGTG AAGCGAGCTG ATACTGAGCA GCTCCTGCTA 
GTGGCCACAG AGCGAATAAA CAGCCTCTCC TCTTTACTGA AGACAAATCT GGAGAGTTTG 
GTCACATTTG CAGGGACAGA TCTTGAAGGC GGAGTCTGTC AGCATCCCAC AATTCCTTCA 
AAAGAAGTAC CTTTGCTGCC AGCTAGTCAT GTCACCATGA CTAAGGGAAC AGGATTGGTC 
CATACAGCTC CAGCCCATGG CATGGATGAC TACAGTGTCG CCACACACTT CAATCTACCA 
GTAGAGTGCA TGGTGGATGA AGAGGGCAGG TTTACTGAAC TTGCTGGAGC TGAACTGCAG 
AATAAATCTG TGATGGACGA AGGCAATGCT GCAGTAATCT CAGTGCTGCA GGCGGCAGGT 
GCAGTAGTGA AGGAGGAGGA CTGTGTCCAC AGTTACCCGT ATGACTGGAG GACCAAACAG 
CCAGTCGTGA TCCGAGCCAG TAGACAGTGG TTTATTAATA CTGCCACCCT CAAAGATAAA 
GCAAAGGATG CCCTGCAGAA AGTGCGTGTG ATACCCGAAT CTGCGAGGTC CAGTTTGTTG 
TCAATGTTGG ACAGAAGAAC ATACTGGTGT ATATCTCGAC AGAGGAGCTG GGGCGTCCCT 
ATCCCAGTGT TCTACCATAA AGAGACTGGA GAGCCACTCT TAAACAAACA TACAGTGACC 
CACATCGCTA AAGTTTTCTC TGAGAAGGGG AGCGACAGCT GGTGGACAGA GCCAGTGGAG 
ACGTTTTTGA CACCAGATGT TCTTCAGAAG AGTAAAGCAA GTTCAGTGTC TGATTATGTG 
CGGGGTGAAG ATGTCCTGGA TATCTGGTTT GACAGTGGGG CTTCATGGGC CGCTGTACTG 
CCAGAATCAG ACCCGCGTGC AGACTCGTAT GTCGAGGGGA AAGATCAGAT CGGCGGCTGG 
TTTCAGTCGT CTCTCCTCAC CAGTGTTGCT GTGCGGAATA AAGCTCCTTA CAAAGCTCTA 
GTTGTTCATG GCTTTGCCGT GAGTGAGAAA GGAGAGAAAA TGTCTAAGTC CATAGGCAAC 
GTGGTGGACC CAGATGTGGT TATTAATGGG GGGAAGGACT CGTCTGTTTC TCCTCCATAT 
GGTGCTGATG TTTTGAGATG GTGGGTTGCA GAGTCCAATG TCTTTTCTGA GGTTCAGATC 
GGACCAAATG CACTGAACGC AGCTAAAGAC AGCATCAACA AGCTGAGGAA CACACTGAAG 
TTTCTCTTAG GCAATCTCCA GGGCTTTGAT CCTCGTTCTC AGGCCGTGGA CCCCAAACAA 
ATGCACTACA TTGACCAGTA CATGCTGCAT CTGCTCCGAG AGTTCAGCAT GAAGGTAACC 
GATGCTTACA GTGAGTTCAA CTATGGCCTT GTCATCCGTC TGCTGCAATC CTTCACCGCT 
AGAGAATTAT CCAACTTCTA TTTCAGCATC ATCAAAGATC GGCTATATTG TGACCCAGAG 
GACTCTGTTG GTCGGCGGTC GTGTCAGACA GTCCTGGAGG AGATTCTGGA TGGAGTGAGT 
CGCTCTATCG CTCCAGTCCT CCCTCATCTA GCTGAGGAGG TGTACCTGCA CGCTCCTGGA 
CATGATGAGG GGAACACTTT GTTTCGGAGC GGCTGGATTA ACACCAGTCC TGTGTGGAGA 
AAACCGGGAC TGGAGGAAGC AGTGGAGGGC GCGTGTGCTA TTAGAGACTC CTTCCTGTCA 
TCTATTCCAG GACGAAATGC TGCCGAGTTT GACCTCGTCA TTTCCATTGA ACCCGGCCTG 
TTCTTTGAGC TCATGGAGTC TCTTCAAGAG GAGCCCACTT CCACCAGCTC TCAGCTGACA 
GAGCTCATGA TGACCTCGCG CACCACTCTG ACAAGCGCTC TCCCACGAGA CCTGCCATCC 
GATGCCATCA CAAGCACGGG GAGCTTCCTC ATCAACCTGG AAGGAGGCAT GATCCGTGAG 
GAAAGCTCAT ACAGCATAGC AGCGATGCCC ACCGCTCTGT CGCGATGCCC GAGATGCCGA 
CGCTACACAT CCGATGAGCC AGACTGCCTC TGTCCGCGAT GCCAAACTAT GATGGAAAAA 
AATGGCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

iars2 ( XM_021467083.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322758.1 Danio rerio isoleucyl-tRNA synthetase 2, mitochondrial (iars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149498
Clone ID Related Accession (Same CDS sequence)	XM_021467083.1
Accession Version	XM_021467083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	isoleucine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG TTCGGCGTTC GGCAGCAGGT TTCGGCGGCG TGGTGCGATG GGGACTGCGC 
TGTCTGTGCT CCGGGGACGC GGCGGGCCGG TACCGAAACA CCGTTCTGCT GCCCCGCACT 
GACTTCCCTA TAAAAGTGAA TGGGCCAAGC CTTCTAGAGC AAGAGATAAA GATCCAGCAG 
AAATGTGGTT TTGACCAGCT GTACAAATGG CAGAGAGAGA AAAAGGCTAA GAAGGAGTAC 
TGTCTTCATG ATGGACCACC GTATGCCAAT GGGGACCCTC ATGTTGGGCA TGCACTCAAT 
AAGATTCTCA AAGACATACG AAACCGTTTC GAGGTGCTGA GAGGCAGGCA GGTGCACTAT 
GTGCCCGGCT GGGACTGTCA TGGTCTGCCC ATTGAACTCA AAGCGCTGGG AGACCTGGGA 
ACCAATGAGC TGACTCCTGT CCAGATCAGA CAGAAAGCTC GTGAGTTTGC AGAGAAGGCC 
ATATCTCGAC AGCGGGCTGC TTTCCAGCGC TGGGGTGTGA TGGCTGACTG GGACAACTGT 
TATTACACTT TTGATGGGAA ATATGAAGCA GCCCAGCTCA AGGTCTTCCA GGAGATGCAC 
AACAAGGGTT TGATTTATCA AGACTATAAG CCTGTTTTCT GGTCACCGTC GTCCAGGACG 
GCGCTTGCAG AAGCTGAGCT GGAGTATAAT CCAGAACATG TGAGCCGTTC TCTGTACATC 
ACTTTCCCCC TGCTCACCCT GCCAGCTAAA CTCACAGCAA AAGCAGGTTT GAGTAATGTG 
TCTGCGCTGA TATGGACAAC GCAACCATGG ACTATTCCAG CCAATCAGGC AGTTTGTTAT 
ATGCCTAAAG TACAGTATTC TGTAGTGAAG CGAGCTGATA CTGAGCAGCT CCTGCTAGTG 
GCCACAGAGC GAATAAACAG CCTCTCCTCT TTACTGAAGA CAAATCTGGA GAGTTTGGTC 
ACATTTGCAG GGACAGATCT TGAAGGCGGA GTCTGTCAGC ATCCCACAAT TCCTTCAAAA 
GAAGTACCTT TGCTGCCAGC TAGTCATGTC ACCATGACTA AGGGAACAGG ATTGGTCCAT 
ACAGCTCCAG CCCATGGCAT GGATGACTAC AGTGTCGCCA CACACTTCAA TCTACCAGTA 
GAGTGCATGG TGGATGAAGA GGGCAGGTTT ACTGAACTTG CTGGAGCTGA ACTGCAGAAT 
AAATCTGTGA TGGACGAAGG CAATGCTGCA GTAATCTCAG TGCTGCAGGC GGCAGGTGCA 
GTAGTGAAGG AGGAGGACTG TGTCCACAGT TACCCGTATG ACTGGAGGAC CAAACAGCCA 
GTCGTGATCC GAGCCAGTAG ACAGTGGTTT ATTAATACTG CCACCCTCAA AGATAAAGCA 
AAGGATGCCC TGCAGAAAGT GCGTGTGATA CCCGAATCTG CGAGGTCCAG TTTGTTGTCA 
ATGTTGGACA GAAGAACATA CTGGTGTATA TCTCGACAGA GGAGCTGGGG CGTCCCTATC 
CCAGTGTTCT ACCATAAAGA GACTGGAGAG CCACTCTTAA ACAAACATAC AGTGACCCAC 
ATCGCTAAAG TTTTCTCTGA GAAGGGGAGC GACAGCTGGT GGACAGAGCC AGTGGAGACG 
TTTTTGACAC CAGATGTTCT TCAGAAGAGT AAAGCAAGTT CAGTGTCTGA TTATGTGCGG 
GGTGAAGATG TCCTGGATAT CTGGTTTGAC AGTGGGGCTT CATGGGCCGC TGTACTGCCA 
GAATCAGACC CGCGTGCAGA CTCGTATGTC GAGGGGAAAG ATCAGATCGG CGGCTGGTTT 
CAGTCGTCTC TCCTCACCAG TGTTGCTGTG CGGAATAAAG CTCCTTACAA AGCTCTAGTT 
GTTCATGGCT TTGCCGTGAG TGAGAAAGGA GAGAAAATGT CTAAGTCCAT AGGCAACGTG 
GTGGACCCAG ATGTGGTTAT TAATGGGGGG AAGGACTCGT CTGTTTCTCC TCCATATGGT 
GCTGATGTTT TGAGATGGTG GGTTGCAGAG TCCAATGTCT TTTCTGAGGT TCAGATCGGA 
CCAAATGCAC TGAACGCAGC TAAAGACAGC ATCAACAAGC TGAGGAACAC ACTGAAGTTT 
CTCTTAGGCA ATCTCCAGGG CTTTGATCCT CGTTCTCAGG CCGTGGACCC CAAACAAATG 
CACTACATTG ACCAGTACAT GCTGCATCTG CTCCGAGAGT TCAGCATGAA GGTAACCGAT 
GCTTACAGTG AGTTCAACTA TGGCCTTGTC ATCCGTCTGC TGCAATCCTT CACCGCTAGA 
GAATTATCCA ACTTCTATTT CAGCATCATC AAAGATCGGC TATATTGTGA CCCAGAGGAC 
TCTGTTGGTC GGCGGTCGTG TCAGACAGTC CTGGAGGAGA TTCTGGATGG AGTGAGTCGC 
TCTATCGCTC CAGTCCTCCC TCATCTAGCT GAGGAGGTGT ACCTGCACGC TCCTGGACAT 
GATGAGGGGA ACACTTTGTT TCGGAGCGGC TGGATTAACA CCAGTCCTGT GTGGAGAAAA 
CCGGGACTGG AGGAAGCAGT GGAGGGCGCG TGTGCTATTA GAGACTCCTT CCTGTCATCT 
ATTCCAGGAC GAAATGCTGC CGAGTTTGAC CTCGTCATTT CCATTGAACC CGGCCTGTTC 
TTTGAGCTCA TGGAGTCTCT TCAAGAGGAG CCCACTTCCA CCAGCTCTCA GCTGACAGAG 
CTCATGATGA CCTCGCGCAC CACTCTGACA AGCGCTCTCC CACGAGACCT GCCATCCGAT 
GCCATCACAA GCACGGGGAG CTTCCTCATC AACCTGGAAG GAGGCATGAT CCGTGAGGAA 
AGCTCATACA GCATAGCAGC GATGCCCACC GCTCTGTCGC GATGCCCGAG ATGCCGACGC 
TACACATCCG ATGAGCCAGA CTGCCTCTGT CCGCGATGCC AAACTATGAT GGAAAAAAAT 
GGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322758.1
CDS	113..3061
Translation	MLLVRRSAAGFGGVVRWGLRCLCSGDAAGRYRNTVLLPRTDFPIKVNGPSLLEQEIKIQQKCGFDQLYKWQREKKAKKEYCLHDGPPYANGDPHVGHALNKILKDIRNRFEVLRGRQVHYVPGWDCHGLPIELKALGDLGTNELTPVQIRQKAREFAEKAISRQRAAFQRWGVMADWDNCYYTFDGKYEAAQLKVFQEMHNKGLIYQDYKPVFWSPSSRTALAEAELEYNPEHVSRSLYITFPLLTLPAKLTAKAGLSNVSALIWTTQPWTIPANQAVCYMPKVQYSVVKRADTEQLLLVATERINSLSSLLKTNLESLVTFAGTDLEGGVCQHPTIPSKEVPLLPASHVTMTKGTGLVHTAPAHGMDDYSVATHFNLPVECMVDEEGRFTELAGAELQNKSVMDEGNAAVISVLQAAGAVVKEEDCVHSYPYDWRTKQPVVIRASRQWFINTATLKDKAKDALQKVRVIPESARSSLLSMLDRRTYWCISRQRSWGVPIPVFYHKETGEPLLNKHTVTHIAKVFSEKGSDSWWTEPVETFLTPDVLQKSKASSVSDYVRGEDVLDIWFDSGASWAAVLPESDPRADSYVEGKDQIGGWFQSSLLTSVAVRNKAPYKALVVHGFAVSEKGEKMSKSIGNVVDPDVVINGGKDSSVSPPYGADVLRWWVAESNVFSEVQIGPNALNAAKDSINKLRNTLKFLLGNLQGFDPRSQAVDPKQMHYIDQYMLHLLREFSMKVTDAYSEFNYGLVIRLLQSFTARELSNFYFSIIKDRLYCDPEDSVGRRSCQTVLEEILDGVSRSIAPVLPHLAEEVYLHAPGHDEGNTLFRSGWINTSPVWRKPGLEEAVEGACAIRDSFLSSIPGRNAAEFDLVISIEPGLFFELMESLQEEPTSTSSQLTELMMTSRTTLTSALPRDLPSDAITSTGSFLINLEGGMIREESSYSIAAMPTALSRCPRCRRYTSDEPDCLCPRCQTMMEKNGK

Target ORF information:

RefSeq Version	XM_021467083.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio isoleucyl-tRNA synthetase 2, mitochondrial (iars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467083.1

ORF Insert Sequence:

ATGCTGCTGG TTCGGCGTTC GGCAGCAGGT TTCGGCGGCG TGGTGCGATG GGGACTGCGC 
TGTCTGTGCT CCGGGGACGC GGCGGGCCGG TACCGAAACA CCGTTCTGCT GCCCCGCACT 
GACTTCCCTA TAAAAGTGAA TGGGCCAAGC CTTCTAGAGC AAGAGATAAA GATCCAGCAG 
AAATGTGGTT TTGACCAGCT GTACAAATGG CAGAGAGAGA AAAAGGCTAA GAAGGAGTAC 
TGTCTTCATG ATGGACCACC GTATGCCAAT GGGGACCCTC ATGTTGGGCA TGCACTCAAT 
AAGATTCTCA AAGACATACG AAACCGTTTC GAGGTGCTGA GAGGCAGGCA GGTGCACTAT 
GTGCCCGGCT GGGACTGTCA TGGTCTGCCC ATTGAACTCA AAGCGCTGGG AGACCTGGGA 
ACCAATGAGC TGACTCCTGT CCAGATCAGA CAGAAAGCTC GTGAGTTTGC AGAGAAGGCC 
ATATCTCGAC AGCGGGCTGC TTTCCAGCGC TGGGGTGTGA TGGCTGACTG GGACAACTGT 
TATTACACTT TTGATGGGAA ATATGAAGCA GCCCAGCTCA AGGTCTTCCA GGAGATGCAC 
AACAAGGGTT TGATTTATCA AGACTATAAG CCTGTTTTCT GGTCACCGTC GTCCAGGACG 
GCGCTTGCAG AAGCTGAGCT GGAGTATAAT CCAGAACATG TGAGCCGTTC TCTGTACATC 
ACTTTCCCCC TGCTCACCCT GCCAGCTAAA CTCACAGCAA AAGCAGGTTT GAGTAATGTG 
TCTGCGCTGA TATGGACAAC GCAACCATGG ACTATTCCAG CCAATCAGGC AGTTTGTTAT 
ATGCCTAAAG TACAGTATTC TGTAGTGAAG CGAGCTGATA CTGAGCAGCT CCTGCTAGTG 
GCCACAGAGC GAATAAACAG CCTCTCCTCT TTACTGAAGA CAAATCTGGA GAGTTTGGTC 
ACATTTGCAG GGACAGATCT TGAAGGCGGA GTCTGTCAGC ATCCCACAAT TCCTTCAAAA 
GAAGTACCTT TGCTGCCAGC TAGTCATGTC ACCATGACTA AGGGAACAGG ATTGGTCCAT 
ACAGCTCCAG CCCATGGCAT GGATGACTAC AGTGTCGCCA CACACTTCAA TCTACCAGTA 
GAGTGCATGG TGGATGAAGA GGGCAGGTTT ACTGAACTTG CTGGAGCTGA ACTGCAGAAT 
AAATCTGTGA TGGACGAAGG CAATGCTGCA GTAATCTCAG TGCTGCAGGC GGCAGGTGCA 
GTAGTGAAGG AGGAGGACTG TGTCCACAGT TACCCGTATG ACTGGAGGAC CAAACAGCCA 
GTCGTGATCC GAGCCAGTAG ACAGTGGTTT ATTAATACTG CCACCCTCAA AGATAAAGCA 
AAGGATGCCC TGCAGAAAGT GCGTGTGATA CCCGAATCTG CGAGGTCCAG TTTGTTGTCA 
ATGTTGGACA GAAGAACATA CTGGTGTATA TCTCGACAGA GGAGCTGGGG CGTCCCTATC 
CCAGTGTTCT ACCATAAAGA GACTGGAGAG CCACTCTTAA ACAAACATAC AGTGACCCAC 
ATCGCTAAAG TTTTCTCTGA GAAGGGGAGC GACAGCTGGT GGACAGAGCC AGTGGAGACG 
TTTTTGACAC CAGATGTTCT TCAGAAGAGT AAAGCAAGTT CAGTGTCTGA TTATGTGCGG 
GGTGAAGATG TCCTGGATAT CTGGTTTGAC AGTGGGGCTT CATGGGCCGC TGTACTGCCA 
GAATCAGACC CGCGTGCAGA CTCGTATGTC GAGGGGAAAG ATCAGATCGG CGGCTGGTTT 
CAGTCGTCTC TCCTCACCAG TGTTGCTGTG CGGAATAAAG CTCCTTACAA AGCTCTAGTT 
GTTCATGGCT TTGCCGTGAG TGAGAAAGGA GAGAAAATGT CTAAGTCCAT AGGCAACGTG 
GTGGACCCAG ATGTGGTTAT TAATGGGGGG AAGGACTCGT CTGTTTCTCC TCCATATGGT 
GCTGATGTTT TGAGATGGTG GGTTGCAGAG TCCAATGTCT TTTCTGAGGT TCAGATCGGA 
CCAAATGCAC TGAACGCAGC TAAAGACAGC ATCAACAAGC TGAGGAACAC ACTGAAGTTT 
CTCTTAGGCA ATCTCCAGGG CTTTGATCCT CGTTCTCAGG CCGTGGACCC CAAACAAATG 
CACTACATTG ACCAGTACAT GCTGCATCTG CTCCGAGAGT TCAGCATGAA GGTAACCGAT 
GCTTACAGTG AGTTCAACTA TGGCCTTGTC ATCCGTCTGC TGCAATCCTT CACCGCTAGA 
GAATTATCCA ACTTCTATTT CAGCATCATC AAAGATCGGC TATATTGTGA CCCAGAGGAC 
TCTGTTGGTC GGCGGTCGTG TCAGACAGTC CTGGAGGAGA TTCTGGATGG AGTGAGTCGC 
TCTATCGCTC CAGTCCTCCC TCATCTAGCT GAGGAGGTGT ACCTGCACGC TCCTGGACAT 
GATGAGGGGA ACACTTTGTT TCGGAGCGGC TGGATTAACA CCAGTCCTGT GTGGAGAAAA 
CCGGGACTGG AGGAAGCAGT GGAGGGCGCG TGTGCTATTA GAGACTCCTT CCTGTCATCT 
ATTCCAGGAC GAAATGCTGC CGAGTTTGAC CTCGTCATTT CCATTGAACC CGGCCTGTTC 
TTTGAGCTCA TGGAGTCTCT TCAAGAGGAG CCCACTTCCA CCAGCTCTCA GCTGACAGAG 
CTCATGATGA CCTCGCGCAC CACTCTGACA AGCGCTCTCC CACGAGACCT GCCATCCGAT 
GCCATCACAA GCACGGGGAG CTTCCTCATC AACCTGGAAG GAGGCATGAT CCGTGAGGAA 
AGCTCATACA GCATAGCAGC GATGCCCACC GCTCTGTCGC GATGCCCGAG ATGCCGACGC 
TACACATCCG ATGAGCCAGA CTGCCTCTGT CCGCGATGCC AAACTATGAT GGAAAAAAAT 
GGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Reconstruction of a 450-My-old ancestral vertebrate protokaryotype.
Trends in genetics : TIG22(4)203-10(2006 Apr)
Kohn M,Högel J,Vogel W,Minich P,Kehrer-Sawatzki H,Graves JA,Hameister H

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iars2 cDNA ORF clone, Danio rerio(zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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