Ube2o cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2o
Entrez Gene ID	100723966
Full Name	ubiquitin conjugating enzyme E2 O
Gene Type	protein-coding
Organism	Cavia porcellus(domestic guinea pig)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003461440.4	XP_003461488.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
XM_004999622.3	XP_004999679.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
XM_003461440.3	XP_003461488.1	E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X2
XM_004999622.2	XP_004999679.1	E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X1
XM_023564973.1	XP_023420741.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3

Pathways from BioSystems
Gene Ontology

The following Ube2o gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2o cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODo01433	XM_004999622.3 Latest version!	Cavia porcellus ubiquitin conjugating enzyme E2 O (Ube2o), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODo01434	XM_003461440.4 Latest version!	Cavia porcellus ubiquitin conjugating enzyme E2 O (Ube2o), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODo41169	XM_023564973.1 Latest version!	Cavia porcellus ubiquitin conjugating enzyme E2 O (Ube2o), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODo01434	XM_003461440.3	Cavia porcellus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODo01433	XM_004999622.2	Cavia porcellus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2o ( XM_004999622.3 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_004999679.1 Cavia porcellus ubiquitin conjugating enzyme E2 O (Ube2o), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODo01433
Clone ID Related Accession (Same CDS sequence)	XM_004999622.3 , XM_004999622.2
Accession Version	XM_004999622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176226.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_004999622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTAGAG CCAGCTAGGA TTGCCTGGGA ATGTCCAGAA 
AAACACTGCG CCCCGGGGGA GGGCTCTATG GCCAAGAAGG TGAAGCGCCT TTTGAAGAAG 
CAGGTCGTGA GGATCATGTC TTGCTCCCCG GACACCCAGT GTGCCCGGGA CCATTCTATG 
GAGGACCCCG ACAAGAAAGG GGAAGCCAAG GCCAAGAGTG AAACAGGTTC TGCTAGCCCA 
GAGGAGCCAC CTGACAGGTC TGCCAGCCCA GTGGAGATGC AGGATGAGGG CCCTGAGGAG 
CCCCAGGAAA CCGGCCAGCC ACTGCCTCCC TTCCTGCTGA AGGAGGGTGG GGATGATGGG 
CTGCACTCAG CAGAGCAGGA TGCGGATGAC GAGGCTGCTG ATGACACTGA TGACACCAGC 
TCCGTGACCT CCTCTGCCAG CTCCACCACC TCCTCCCAGA GCGGCAGCGG CACAAGTCGC 
AAGAAGAGCA TCCCTTTGTC CATCAAGAAC CTGAAGCGAA AGCACAAGAG GAAGAAGAAT 
AAAATTACTC GTGACTTCAA ACCAGGAGAC AGGGTGGCAG TGGAGGTGGT GACCACCATG 
ACCTCAGCTG ACGTGATGTG GCAGGACGGC TCCGTGGAGT GCAACATCCG CTCCAATGAC 
CTCTTTCCTG TGCACCATCT GGACAACAAT GAGTTCTGCC CTGGAGACTT TGTGGTCGAC 
AAGCGAGTTC AGAGCTGTCC AGACCCTGCT GTGTATGGCG TGGTGCAGTC TGGGGACCAC 
ATCGGCCGCA CCTGCATGGT AAAGTGGTTC AAGCTGCGGC CGAGCGGGGA TGACGTGGAG 
CTGATTGGAG AAGAGGAAGA TGTGAGCGTG TATGACATTG CTGACCACCC TGACTTTCGG 
TTCCGCACGA CCGACATTGT CATTCGAATA GGCAACACTG AAGATGGGGC TGCACACAAT 
GAGGACGAAC AGCCATCAGT GGGTCAGGTG GCCCGTGTGG ACGTCAGCAG CAAGGTGGAG 
GTGGTGTGGG CCGACAACTC GAAGACCATC ATCCTGCCCC AGCACTTGTA CAACATTGAA 
TCCGAGATTG AGGAGTCAGA CTATGATTCA GTGGAAGGCA GCACCAGTGG GGCATCTTCA 
GACGAGTGGG AGGATGACAG TGACAGCTGG GAGACAGACA ATGGACTGGT GGAAGATGAG 
CACCCCAAAA TAGAGGAGCC CCCCATCCTG CCCACTGAGC AGCTGTCAGC ACCCGAGGAG 
GACAAAGGCG TAGTGATCAG TGAAGAGGCT GCCACCGCTG CCATCCAGGG GGCCGTGGCC 
ATGCCTGCTC CCGTGGCAGG ACTGATGGAG AAGGCTGGCA AGGATGGGCC CCCCAAGAGC 
TTCCGAGAGC TGAAGGAGGC CATCAAGATC CTGGAGAGCC TCAAAAACAT GACAGTGGAG 
CAGCTGCTCA CTGGCTCTCC CACTTCCCCC ACTGTGGAGC CTGAGAAACC CACCAGAGAG 
AAGAAGTTCT TGGACGACAT TAAGAAGCTG CAGGAGAACC TCAAGAAGAC CCTGGACAAC 
GTGGCCATCG CAGAGGAGGA GAAAATGGAG GCAGCACCTG ACACCGAGCG CAAGGAGGAC 
AAGCCTGAAG GGCAGTCGCC AGTGAAGGCC GAGTGGCCCA GCGAGACCCC TGTGCTCTGC 
CAGCAGTGTG GTGGCAAGCC CGGTGTCACC TTCACCAGCG CCAAAGGCGA GGTCTTCTCC 
GTGCTGGAGT TTGCACCTTC AAATCACTCT TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA 
GCCAAGAAGT TCTTCAGCAC GGTGCGGAAG GAGATGGCAC TGCTGGCTAC CTCACTGCCC 
GATGGCATCA TGGTCAAGAC TTTTGAGGAT AGAATGGACC TCTTCTCAGC TCTAATCAAG 
GGCCCCACTC GAACCCCCTA CGAAGACGGC CTCTACCTGT TTGACATCCA GCTCCCCAAC 
ATCTACCCAG CTGTGCCCCC CCACTTTTGC TACCTCTCCC AGTGCAGTGG CCGCCTGAAC 
CCCAACCTGT ATGACAATGG GAAAGTGTGT GTCAGCCTCC TGGGCACCTG GATTGGAAAG 
GGGACAGAGA GGTGGACAAG TAAATCCAGC CTACTCCAGG TGCTCATCTC TATCCAAGGT 
CTGATCCTGG TGAACGAACC TTACTACAAT GAAGCCGGCT TCGACAGTGA CCGGGGCCTG 
CAGGAAGGCT ATGAGAACAG TCGCTGCTAT AATGAGATGG CACTGATCCG TGTGGTACAG 
TCCATGACAC AGCTGGTTCG GCGGCCCCCC GAGGTCTTTG AGCAGGAGAT CCGGCAGCAC 
TTTAGCACCG GTGGCTGGCG GCTGGTGAAC CGCATCGAGT CCTGGTTGGA AACCCACGCG 
CTACTAGAGA GGGCCCAGGC GCTACCCAAT GGGGTCGCCA AGGACAGCAG CTCTCCAGAG 
ACAGCTGCTA CGGCCGAGCT CTCAGACTCT GGCCGAGAAG AACCTGAGGA TGTCGGAATG 
GCCCCTGGAG AGGCCTCCCA GGGCTCAGAC TCAGAGGGGG CTGTCCAGGG CCCGGCCTCC 
ACTAGCAGGG ACCACACAGA TCAGACTTCG GAGGTGGCAC CTGATGCATC CGTACCACCC 
AGCGTCAAAC CAAAGAAGCG GAGAAAGAGC TATCGGAGCT TCTTGCCTGA GAAGAGCGGC 
TACCCTGACA TCGGCTTTCC CCTCTTCCCA CTCTCTAAGG GTTTCATCAA AAGCATCAGG 
GGCGTCCTGA CGCAGTTCCG GGCTGCGCTG CTAGAGGCAG GCATGCCTGA AAGCACAGAG 
AACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004999679.1
CDS	69..3977
Translation	MADPAASAPAAPAPPPAQAPAPEAASARAAAHSLTPAQASVPTPAPASDSASVPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDICPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPARIAWECPEKHCAPGEGSMAKKVKRLLKKQVVRIMSCSPDTQCARDHSMEDPDKKGEAKAKSETGSASPEEPPDRSASPVEMQDEGPEEPQETGQPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAAHNEDEQPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPILPTEQLSAPEEDKGVVISEEAATAAIQGAVAMPAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDTERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGVAKDSSSPETAATAELSDSGREEPEDVGMAPGEASQGSDSEGAVQGPASTSRDHTDQTSEVAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTENK

Target ORF information:

RefSeq Version	XM_004999622.3
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus ubiquitin conjugating enzyme E2 O (Ube2o), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004999622.3

ORF Insert Sequence:

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTAGAG CCAGCTAGGA TTGCCTGGGA ATGTCCAGAA 
AAACACTGCG CCCCGGGGGA GGGCTCTATG GCCAAGAAGG TGAAGCGCCT TTTGAAGAAG 
CAGGTCGTGA GGATCATGTC TTGCTCCCCG GACACCCAGT GTGCCCGGGA CCATTCTATG 
GAGGACCCCG ACAAGAAAGG GGAAGCCAAG GCCAAGAGTG AAACAGGTTC TGCTAGCCCA 
GAGGAGCCAC CTGACAGGTC TGCCAGCCCA GTGGAGATGC AGGATGAGGG CCCTGAGGAG 
CCCCAGGAAA CCGGCCAGCC ACTGCCTCCC TTCCTGCTGA AGGAGGGTGG GGATGATGGG 
CTGCACTCAG CAGAGCAGGA TGCGGATGAC GAGGCTGCTG ATGACACTGA TGACACCAGC 
TCCGTGACCT CCTCTGCCAG CTCCACCACC TCCTCCCAGA GCGGCAGCGG CACAAGTCGC 
AAGAAGAGCA TCCCTTTGTC CATCAAGAAC CTGAAGCGAA AGCACAAGAG GAAGAAGAAT 
AAAATTACTC GTGACTTCAA ACCAGGAGAC AGGGTGGCAG TGGAGGTGGT GACCACCATG 
ACCTCAGCTG ACGTGATGTG GCAGGACGGC TCCGTGGAGT GCAACATCCG CTCCAATGAC 
CTCTTTCCTG TGCACCATCT GGACAACAAT GAGTTCTGCC CTGGAGACTT TGTGGTCGAC 
AAGCGAGTTC AGAGCTGTCC AGACCCTGCT GTGTATGGCG TGGTGCAGTC TGGGGACCAC 
ATCGGCCGCA CCTGCATGGT AAAGTGGTTC AAGCTGCGGC CGAGCGGGGA TGACGTGGAG 
CTGATTGGAG AAGAGGAAGA TGTGAGCGTG TATGACATTG CTGACCACCC TGACTTTCGG 
TTCCGCACGA CCGACATTGT CATTCGAATA GGCAACACTG AAGATGGGGC TGCACACAAT 
GAGGACGAAC AGCCATCAGT GGGTCAGGTG GCCCGTGTGG ACGTCAGCAG CAAGGTGGAG 
GTGGTGTGGG CCGACAACTC GAAGACCATC ATCCTGCCCC AGCACTTGTA CAACATTGAA 
TCCGAGATTG AGGAGTCAGA CTATGATTCA GTGGAAGGCA GCACCAGTGG GGCATCTTCA 
GACGAGTGGG AGGATGACAG TGACAGCTGG GAGACAGACA ATGGACTGGT GGAAGATGAG 
CACCCCAAAA TAGAGGAGCC CCCCATCCTG CCCACTGAGC AGCTGTCAGC ACCCGAGGAG 
GACAAAGGCG TAGTGATCAG TGAAGAGGCT GCCACCGCTG CCATCCAGGG GGCCGTGGCC 
ATGCCTGCTC CCGTGGCAGG ACTGATGGAG AAGGCTGGCA AGGATGGGCC CCCCAAGAGC 
TTCCGAGAGC TGAAGGAGGC CATCAAGATC CTGGAGAGCC TCAAAAACAT GACAGTGGAG 
CAGCTGCTCA CTGGCTCTCC CACTTCCCCC ACTGTGGAGC CTGAGAAACC CACCAGAGAG 
AAGAAGTTCT TGGACGACAT TAAGAAGCTG CAGGAGAACC TCAAGAAGAC CCTGGACAAC 
GTGGCCATCG CAGAGGAGGA GAAAATGGAG GCAGCACCTG ACACCGAGCG CAAGGAGGAC 
AAGCCTGAAG GGCAGTCGCC AGTGAAGGCC GAGTGGCCCA GCGAGACCCC TGTGCTCTGC 
CAGCAGTGTG GTGGCAAGCC CGGTGTCACC TTCACCAGCG CCAAAGGCGA GGTCTTCTCC 
GTGCTGGAGT TTGCACCTTC AAATCACTCT TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA 
GCCAAGAAGT TCTTCAGCAC GGTGCGGAAG GAGATGGCAC TGCTGGCTAC CTCACTGCCC 
GATGGCATCA TGGTCAAGAC TTTTGAGGAT AGAATGGACC TCTTCTCAGC TCTAATCAAG 
GGCCCCACTC GAACCCCCTA CGAAGACGGC CTCTACCTGT TTGACATCCA GCTCCCCAAC 
ATCTACCCAG CTGTGCCCCC CCACTTTTGC TACCTCTCCC AGTGCAGTGG CCGCCTGAAC 
CCCAACCTGT ATGACAATGG GAAAGTGTGT GTCAGCCTCC TGGGCACCTG GATTGGAAAG 
GGGACAGAGA GGTGGACAAG TAAATCCAGC CTACTCCAGG TGCTCATCTC TATCCAAGGT 
CTGATCCTGG TGAACGAACC TTACTACAAT GAAGCCGGCT TCGACAGTGA CCGGGGCCTG 
CAGGAAGGCT ATGAGAACAG TCGCTGCTAT AATGAGATGG CACTGATCCG TGTGGTACAG 
TCCATGACAC AGCTGGTTCG GCGGCCCCCC GAGGTCTTTG AGCAGGAGAT CCGGCAGCAC 
TTTAGCACCG GTGGCTGGCG GCTGGTGAAC CGCATCGAGT CCTGGTTGGA AACCCACGCG 
CTACTAGAGA GGGCCCAGGC GCTACCCAAT GGGGTCGCCA AGGACAGCAG CTCTCCAGAG 
ACAGCTGCTA CGGCCGAGCT CTCAGACTCT GGCCGAGAAG AACCTGAGGA TGTCGGAATG 
GCCCCTGGAG AGGCCTCCCA GGGCTCAGAC TCAGAGGGGG CTGTCCAGGG CCCGGCCTCC 
ACTAGCAGGG ACCACACAGA TCAGACTTCG GAGGTGGCAC CTGATGCATC CGTACCACCC 
AGCGTCAAAC CAAAGAAGCG GAGAAAGAGC TATCGGAGCT TCTTGCCTGA GAAGAGCGGC 
TACCCTGACA TCGGCTTTCC CCTCTTCCCA CTCTCTAAGG GTTTCATCAA AAGCATCAGG 
GGCGTCCTGA CGCAGTTCCG GGCTGCGCTG CTAGAGGCAG GCATGCCTGA AAGCACAGAG 
AACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2o ( XM_004999622.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_004999679.1 Cavia porcellus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODo01433
Clone ID Related Accession (Same CDS sequence)	XM_004999622.3 , XM_004999622.2
Accession Version	XM_004999622.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176226.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 14, 2015 this sequence version replaced XM_004999622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTAGAG CCAGCTAGGA TTGCCTGGGA ATGTCCAGAA 
AAACACTGCG CCCCGGGGGA GGGCTCTATG GCCAAGAAGG TGAAGCGCCT TTTGAAGAAG 
CAGGTCGTGA GGATCATGTC TTGCTCCCCG GACACCCAGT GTGCCCGGGA CCATTCTATG 
GAGGACCCCG ACAAGAAAGG GGAAGCCAAG GCCAAGAGTG AAACAGGTTC TGCTAGCCCA 
GAGGAGCCAC CTGACAGGTC TGCCAGCCCA GTGGAGATGC AGGATGAGGG CCCTGAGGAG 
CCCCAGGAAA CCGGCCAGCC ACTGCCTCCC TTCCTGCTGA AGGAGGGTGG GGATGATGGG 
CTGCACTCAG CAGAGCAGGA TGCGGATGAC GAGGCTGCTG ATGACACTGA TGACACCAGC 
TCCGTGACCT CCTCTGCCAG CTCCACCACC TCCTCCCAGA GCGGCAGCGG CACAAGTCGC 
AAGAAGAGCA TCCCTTTGTC CATCAAGAAC CTGAAGCGAA AGCACAAGAG GAAGAAGAAT 
AAAATTACTC GTGACTTCAA ACCAGGAGAC AGGGTGGCAG TGGAGGTGGT GACCACCATG 
ACCTCAGCTG ACGTGATGTG GCAGGACGGC TCCGTGGAGT GCAACATCCG CTCCAATGAC 
CTCTTTCCTG TGCACCATCT GGACAACAAT GAGTTCTGCC CTGGAGACTT TGTGGTCGAC 
AAGCGAGTTC AGAGCTGTCC AGACCCTGCT GTGTATGGCG TGGTGCAGTC TGGGGACCAC 
ATCGGCCGCA CCTGCATGGT AAAGTGGTTC AAGCTGCGGC CGAGCGGGGA TGACGTGGAG 
CTGATTGGAG AAGAGGAAGA TGTGAGCGTG TATGACATTG CTGACCACCC TGACTTTCGG 
TTCCGCACGA CCGACATTGT CATTCGAATA GGCAACACTG AAGATGGGGC TGCACACAAT 
GAGGACGAAC AGCCATCAGT GGGTCAGGTG GCCCGTGTGG ACGTCAGCAG CAAGGTGGAG 
GTGGTGTGGG CCGACAACTC GAAGACCATC ATCCTGCCCC AGCACTTGTA CAACATTGAA 
TCCGAGATTG AGGAGTCAGA CTATGATTCA GTGGAAGGCA GCACCAGTGG GGCATCTTCA 
GACGAGTGGG AGGATGACAG TGACAGCTGG GAGACAGACA ATGGACTGGT GGAAGATGAG 
CACCCCAAAA TAGAGGAGCC CCCCATCCTG CCCACTGAGC AGCTGTCAGC ACCCGAGGAG 
GACAAAGGCG TAGTGATCAG TGAAGAGGCT GCCACCGCTG CCATCCAGGG GGCCGTGGCC 
ATGCCTGCTC CCGTGGCAGG ACTGATGGAG AAGGCTGGCA AGGATGGGCC CCCCAAGAGC 
TTCCGAGAGC TGAAGGAGGC CATCAAGATC CTGGAGAGCC TCAAAAACAT GACAGTGGAG 
CAGCTGCTCA CTGGCTCTCC CACTTCCCCC ACTGTGGAGC CTGAGAAACC CACCAGAGAG 
AAGAAGTTCT TGGACGACAT TAAGAAGCTG CAGGAGAACC TCAAGAAGAC CCTGGACAAC 
GTGGCCATCG CAGAGGAGGA GAAAATGGAG GCAGCACCTG ACACCGAGCG CAAGGAGGAC 
AAGCCTGAAG GGCAGTCGCC AGTGAAGGCC GAGTGGCCCA GCGAGACCCC TGTGCTCTGC 
CAGCAGTGTG GTGGCAAGCC CGGTGTCACC TTCACCAGCG CCAAAGGCGA GGTCTTCTCC 
GTGCTGGAGT TTGCACCTTC AAATCACTCT TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA 
GCCAAGAAGT TCTTCAGCAC GGTGCGGAAG GAGATGGCAC TGCTGGCTAC CTCACTGCCC 
GATGGCATCA TGGTCAAGAC TTTTGAGGAT AGAATGGACC TCTTCTCAGC TCTAATCAAG 
GGCCCCACTC GAACCCCCTA CGAAGACGGC CTCTACCTGT TTGACATCCA GCTCCCCAAC 
ATCTACCCAG CTGTGCCCCC CCACTTTTGC TACCTCTCCC AGTGCAGTGG CCGCCTGAAC 
CCCAACCTGT ATGACAATGG GAAAGTGTGT GTCAGCCTCC TGGGCACCTG GATTGGAAAG 
GGGACAGAGA GGTGGACAAG TAAATCCAGC CTACTCCAGG TGCTCATCTC TATCCAAGGT 
CTGATCCTGG TGAACGAACC TTACTACAAT GAAGCCGGCT TCGACAGTGA CCGGGGCCTG 
CAGGAAGGCT ATGAGAACAG TCGCTGCTAT AATGAGATGG CACTGATCCG TGTGGTACAG 
TCCATGACAC AGCTGGTTCG GCGGCCCCCC GAGGTCTTTG AGCAGGAGAT CCGGCAGCAC 
TTTAGCACCG GTGGCTGGCG GCTGGTGAAC CGCATCGAGT CCTGGTTGGA AACCCACGCG 
CTACTAGAGA GGGCCCAGGC GCTACCCAAT GGGGTCGCCA AGGACAGCAG CTCTCCAGAG 
ACAGCTGCTA CGGCCGAGCT CTCAGACTCT GGCCGAGAAG AACCTGAGGA TGTCGGAATG 
GCCCCTGGAG AGGCCTCCCA GGGCTCAGAC TCAGAGGGGG CTGTCCAGGG CCCGGCCTCC 
ACTAGCAGGG ACCACACAGA TCAGACTTCG GAGGTGGCAC CTGATGCATC CGTACCACCC 
AGCGTCAAAC CAAAGAAGCG GAGAAAGAGC TATCGGAGCT TCTTGCCTGA GAAGAGCGGC 
TACCCTGACA TCGGCTTTCC CCTCTTCCCA CTCTCTAAGG GTTTCATCAA AAGCATCAGG 
GGCGTCCTGA CGCAGTTCCG GGCTGCGCTG CTAGAGGCAG GCATGCCTGA AAGCACAGAG 
AACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004999679.1
CDS	63..3971
Translation	MADPAASAPAAPAPPPAQAPAPEAASARAAAHSLTPAQASVPTPAPASDSASVPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDICPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPARIAWECPEKHCAPGEGSMAKKVKRLLKKQVVRIMSCSPDTQCARDHSMEDPDKKGEAKAKSETGSASPEEPPDRSASPVEMQDEGPEEPQETGQPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAAHNEDEQPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPILPTEQLSAPEEDKGVVISEEAATAAIQGAVAMPAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDTERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGVAKDSSSPETAATAELSDSGREEPEDVGMAPGEASQGSDSEGAVQGPASTSRDHTDQTSEVAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTENK

Target ORF information:

RefSeq Version	XM_004999622.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004999622.2

ORF Insert Sequence:

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTAGAG CCAGCTAGGA TTGCCTGGGA ATGTCCAGAA 
AAACACTGCG CCCCGGGGGA GGGCTCTATG GCCAAGAAGG TGAAGCGCCT TTTGAAGAAG 
CAGGTCGTGA GGATCATGTC TTGCTCCCCG GACACCCAGT GTGCCCGGGA CCATTCTATG 
GAGGACCCCG ACAAGAAAGG GGAAGCCAAG GCCAAGAGTG AAACAGGTTC TGCTAGCCCA 
GAGGAGCCAC CTGACAGGTC TGCCAGCCCA GTGGAGATGC AGGATGAGGG CCCTGAGGAG 
CCCCAGGAAA CCGGCCAGCC ACTGCCTCCC TTCCTGCTGA AGGAGGGTGG GGATGATGGG 
CTGCACTCAG CAGAGCAGGA TGCGGATGAC GAGGCTGCTG ATGACACTGA TGACACCAGC 
TCCGTGACCT CCTCTGCCAG CTCCACCACC TCCTCCCAGA GCGGCAGCGG CACAAGTCGC 
AAGAAGAGCA TCCCTTTGTC CATCAAGAAC CTGAAGCGAA AGCACAAGAG GAAGAAGAAT 
AAAATTACTC GTGACTTCAA ACCAGGAGAC AGGGTGGCAG TGGAGGTGGT GACCACCATG 
ACCTCAGCTG ACGTGATGTG GCAGGACGGC TCCGTGGAGT GCAACATCCG CTCCAATGAC 
CTCTTTCCTG TGCACCATCT GGACAACAAT GAGTTCTGCC CTGGAGACTT TGTGGTCGAC 
AAGCGAGTTC AGAGCTGTCC AGACCCTGCT GTGTATGGCG TGGTGCAGTC TGGGGACCAC 
ATCGGCCGCA CCTGCATGGT AAAGTGGTTC AAGCTGCGGC CGAGCGGGGA TGACGTGGAG 
CTGATTGGAG AAGAGGAAGA TGTGAGCGTG TATGACATTG CTGACCACCC TGACTTTCGG 
TTCCGCACGA CCGACATTGT CATTCGAATA GGCAACACTG AAGATGGGGC TGCACACAAT 
GAGGACGAAC AGCCATCAGT GGGTCAGGTG GCCCGTGTGG ACGTCAGCAG CAAGGTGGAG 
GTGGTGTGGG CCGACAACTC GAAGACCATC ATCCTGCCCC AGCACTTGTA CAACATTGAA 
TCCGAGATTG AGGAGTCAGA CTATGATTCA GTGGAAGGCA GCACCAGTGG GGCATCTTCA 
GACGAGTGGG AGGATGACAG TGACAGCTGG GAGACAGACA ATGGACTGGT GGAAGATGAG 
CACCCCAAAA TAGAGGAGCC CCCCATCCTG CCCACTGAGC AGCTGTCAGC ACCCGAGGAG 
GACAAAGGCG TAGTGATCAG TGAAGAGGCT GCCACCGCTG CCATCCAGGG GGCCGTGGCC 
ATGCCTGCTC CCGTGGCAGG ACTGATGGAG AAGGCTGGCA AGGATGGGCC CCCCAAGAGC 
TTCCGAGAGC TGAAGGAGGC CATCAAGATC CTGGAGAGCC TCAAAAACAT GACAGTGGAG 
CAGCTGCTCA CTGGCTCTCC CACTTCCCCC ACTGTGGAGC CTGAGAAACC CACCAGAGAG 
AAGAAGTTCT TGGACGACAT TAAGAAGCTG CAGGAGAACC TCAAGAAGAC CCTGGACAAC 
GTGGCCATCG CAGAGGAGGA GAAAATGGAG GCAGCACCTG ACACCGAGCG CAAGGAGGAC 
AAGCCTGAAG GGCAGTCGCC AGTGAAGGCC GAGTGGCCCA GCGAGACCCC TGTGCTCTGC 
CAGCAGTGTG GTGGCAAGCC CGGTGTCACC TTCACCAGCG CCAAAGGCGA GGTCTTCTCC 
GTGCTGGAGT TTGCACCTTC AAATCACTCT TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA 
GCCAAGAAGT TCTTCAGCAC GGTGCGGAAG GAGATGGCAC TGCTGGCTAC CTCACTGCCC 
GATGGCATCA TGGTCAAGAC TTTTGAGGAT AGAATGGACC TCTTCTCAGC TCTAATCAAG 
GGCCCCACTC GAACCCCCTA CGAAGACGGC CTCTACCTGT TTGACATCCA GCTCCCCAAC 
ATCTACCCAG CTGTGCCCCC CCACTTTTGC TACCTCTCCC AGTGCAGTGG CCGCCTGAAC 
CCCAACCTGT ATGACAATGG GAAAGTGTGT GTCAGCCTCC TGGGCACCTG GATTGGAAAG 
GGGACAGAGA GGTGGACAAG TAAATCCAGC CTACTCCAGG TGCTCATCTC TATCCAAGGT 
CTGATCCTGG TGAACGAACC TTACTACAAT GAAGCCGGCT TCGACAGTGA CCGGGGCCTG 
CAGGAAGGCT ATGAGAACAG TCGCTGCTAT AATGAGATGG CACTGATCCG TGTGGTACAG 
TCCATGACAC AGCTGGTTCG GCGGCCCCCC GAGGTCTTTG AGCAGGAGAT CCGGCAGCAC 
TTTAGCACCG GTGGCTGGCG GCTGGTGAAC CGCATCGAGT CCTGGTTGGA AACCCACGCG 
CTACTAGAGA GGGCCCAGGC GCTACCCAAT GGGGTCGCCA AGGACAGCAG CTCTCCAGAG 
ACAGCTGCTA CGGCCGAGCT CTCAGACTCT GGCCGAGAAG AACCTGAGGA TGTCGGAATG 
GCCCCTGGAG AGGCCTCCCA GGGCTCAGAC TCAGAGGGGG CTGTCCAGGG CCCGGCCTCC 
ACTAGCAGGG ACCACACAGA TCAGACTTCG GAGGTGGCAC CTGATGCATC CGTACCACCC 
AGCGTCAAAC CAAAGAAGCG GAGAAAGAGC TATCGGAGCT TCTTGCCTGA GAAGAGCGGC 
TACCCTGACA TCGGCTTTCC CCTCTTCCCA CTCTCTAAGG GTTTCATCAA AAGCATCAGG 
GGCGTCCTGA CGCAGTTCCG GGCTGCGCTG CTAGAGGCAG GCATGCCTGA AAGCACAGAG 
AACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2o ( XM_003461440.4 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_003461488.1 Cavia porcellus ubiquitin conjugating enzyme E2 O (Ube2o), transcript variant X2, mRNA.

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CloneID	ODo01434
Clone ID Related Accession (Same CDS sequence)	XM_003461440.4 , XM_003461440.3
Accession Version	XM_003461440.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176226.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_003461440.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTAGAG CCAGCTAGGA TTGCCTGGGA ATGTCCAGAA 
AAACACTGCG CCCCGGGGGA GGGCTCTATG GCCAAGAAGG TGAAGCGCCT TTTGAAGAAG 
CAGGTCGTGA GGATCATGTC TTGCTCCCCG GACACCCAGT GTGCCCGGGA CCATTCTATG 
GAGGACCCCG ACAAGAAAGG GGAAGCCAAG GCCAAGAGTG AAACAGGTTC TGCTAGCCCA 
GAGGAGCCAC CTGACAGGTC TGCCAGCCCA GTGGAGATGC AGGATGAGGG CCCTGAGGAG 
CCCCAGGAAA CCGGCCAGCC ACTGCCTCCC TTCCTGCTGA AGGAGGGTGG GGATGATGGG 
CTGCACTCAG CAGAGCAGGA TGCGGATGAC GAGGCTGCTG ATGACACTGA TGACACCAGC 
TCCGTGACCT CCTCTGCCAG CTCCACCACC TCCTCCCAGA GCGGCAGCGG CACAAGTCGC 
AAGAAGAGCA TCCCTTTGTC CATCAAGAAC CTGAAGCGAA AGCACAAGAG GAAGAAGAAT 
AAAATTACTC GTGACTTCAA ACCAGGAGAC AGGGTGGCAG TGGAGGTGGT GACCACCATG 
ACCTCAGCTG ACGTGATGTG GCAGGACGGC TCCGTGGAGT GCAACATCCG CTCCAATGAC 
CTCTTTCCTG TGCACCATCT GGACAACAAT GAGTTCTGCC CTGGAGACTT TGTGGTCGAC 
AAGCGAGTTC AGAGCTGTCC AGACCCTGCT GTGTATGGCG TGGTGCAGTC TGGGGACCAC 
ATCGGCCGCA CCTGCATGGT AAAGTGGTTC AAGCTGCGGC CGAGCGGGGA TGACGTGGAG 
CTGATTGGAG AAGAGGAAGA TGTGAGCGTG TATGACATTG CTGACCACCC TGACTTTCGG 
TTCCGCACGA CCGACATTGT CATTCGAATA GGCAACACTG AAGATGGGGC TGCACACAAT 
GAGGACGAAC CATCAGTGGG TCAGGTGGCC CGTGTGGACG TCAGCAGCAA GGTGGAGGTG 
GTGTGGGCCG ACAACTCGAA GACCATCATC CTGCCCCAGC ACTTGTACAA CATTGAATCC 
GAGATTGAGG AGTCAGACTA TGATTCAGTG GAAGGCAGCA CCAGTGGGGC ATCTTCAGAC 
GAGTGGGAGG ATGACAGTGA CAGCTGGGAG ACAGACAATG GACTGGTGGA AGATGAGCAC 
CCCAAAATAG AGGAGCCCCC CATCCTGCCC ACTGAGCAGC TGTCAGCACC CGAGGAGGAC 
AAAGGCGTAG TGATCAGTGA AGAGGCTGCC ACCGCTGCCA TCCAGGGGGC CGTGGCCATG 
CCTGCTCCCG TGGCAGGACT GATGGAGAAG GCTGGCAAGG ATGGGCCCCC CAAGAGCTTC 
CGAGAGCTGA AGGAGGCCAT CAAGATCCTG GAGAGCCTCA AAAACATGAC AGTGGAGCAG 
CTGCTCACTG GCTCTCCCAC TTCCCCCACT GTGGAGCCTG AGAAACCCAC CAGAGAGAAG 
AAGTTCTTGG ACGACATTAA GAAGCTGCAG GAGAACCTCA AGAAGACCCT GGACAACGTG 
GCCATCGCAG AGGAGGAGAA AATGGAGGCA GCACCTGACA CCGAGCGCAA GGAGGACAAG 
CCTGAAGGGC AGTCGCCAGT GAAGGCCGAG TGGCCCAGCG AGACCCCTGT GCTCTGCCAG 
CAGTGTGGTG GCAAGCCCGG TGTCACCTTC ACCAGCGCCA AAGGCGAGGT CTTCTCCGTG 
CTGGAGTTTG CACCTTCAAA TCACTCTTTT AAGAAAATTG AGTTCCAGCC TCCAGAAGCC 
AAGAAGTTCT TCAGCACGGT GCGGAAGGAG ATGGCACTGC TGGCTACCTC ACTGCCCGAT 
GGCATCATGG TCAAGACTTT TGAGGATAGA ATGGACCTCT TCTCAGCTCT AATCAAGGGC 
CCCACTCGAA CCCCCTACGA AGACGGCCTC TACCTGTTTG ACATCCAGCT CCCCAACATC 
TACCCAGCTG TGCCCCCCCA CTTTTGCTAC CTCTCCCAGT GCAGTGGCCG CCTGAACCCC 
AACCTGTATG ACAATGGGAA AGTGTGTGTC AGCCTCCTGG GCACCTGGAT TGGAAAGGGG 
ACAGAGAGGT GGACAAGTAA ATCCAGCCTA CTCCAGGTGC TCATCTCTAT CCAAGGTCTG 
ATCCTGGTGA ACGAACCTTA CTACAATGAA GCCGGCTTCG ACAGTGACCG GGGCCTGCAG 
GAAGGCTATG AGAACAGTCG CTGCTATAAT GAGATGGCAC TGATCCGTGT GGTACAGTCC 
ATGACACAGC TGGTTCGGCG GCCCCCCGAG GTCTTTGAGC AGGAGATCCG GCAGCACTTT 
AGCACCGGTG GCTGGCGGCT GGTGAACCGC ATCGAGTCCT GGTTGGAAAC CCACGCGCTA 
CTAGAGAGGG CCCAGGCGCT ACCCAATGGG GTCGCCAAGG ACAGCAGCTC TCCAGAGACA 
GCTGCTACGG CCGAGCTCTC AGACTCTGGC CGAGAAGAAC CTGAGGATGT CGGAATGGCC 
CCTGGAGAGG CCTCCCAGGG CTCAGACTCA GAGGGGGCTG TCCAGGGCCC GGCCTCCACT 
AGCAGGGACC ACACAGATCA GACTTCGGAG GTGGCACCTG ATGCATCCGT ACCACCCAGC 
GTCAAACCAA AGAAGCGGAG AAAGAGCTAT CGGAGCTTCT TGCCTGAGAA GAGCGGCTAC 
CCTGACATCG GCTTTCCCCT CTTCCCACTC TCTAAGGGTT TCATCAAAAG CATCAGGGGC 
GTCCTGACGC AGTTCCGGGC TGCGCTGCTA GAGGCAGGCA TGCCTGAAAG CACAGAGAAC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003461488.1
CDS	69..3974
Translation	MADPAASAPAAPAPPPAQAPAPEAASARAAAHSLTPAQASVPTPAPASDSASVPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDICPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPARIAWECPEKHCAPGEGSMAKKVKRLLKKQVVRIMSCSPDTQCARDHSMEDPDKKGEAKAKSETGSASPEEPPDRSASPVEMQDEGPEEPQETGQPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAAHNEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPILPTEQLSAPEEDKGVVISEEAATAAIQGAVAMPAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDTERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGVAKDSSSPETAATAELSDSGREEPEDVGMAPGEASQGSDSEGAVQGPASTSRDHTDQTSEVAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTENK

Target ORF information:

RefSeq Version	XM_003461440.4
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus ubiquitin conjugating enzyme E2 O (Ube2o), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003461440.4

ORF Insert Sequence:

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTAGAG CCAGCTAGGA TTGCCTGGGA ATGTCCAGAA 
AAACACTGCG CCCCGGGGGA GGGCTCTATG GCCAAGAAGG TGAAGCGCCT TTTGAAGAAG 
CAGGTCGTGA GGATCATGTC TTGCTCCCCG GACACCCAGT GTGCCCGGGA CCATTCTATG 
GAGGACCCCG ACAAGAAAGG GGAAGCCAAG GCCAAGAGTG AAACAGGTTC TGCTAGCCCA 
GAGGAGCCAC CTGACAGGTC TGCCAGCCCA GTGGAGATGC AGGATGAGGG CCCTGAGGAG 
CCCCAGGAAA CCGGCCAGCC ACTGCCTCCC TTCCTGCTGA AGGAGGGTGG GGATGATGGG 
CTGCACTCAG CAGAGCAGGA TGCGGATGAC GAGGCTGCTG ATGACACTGA TGACACCAGC 
TCCGTGACCT CCTCTGCCAG CTCCACCACC TCCTCCCAGA GCGGCAGCGG CACAAGTCGC 
AAGAAGAGCA TCCCTTTGTC CATCAAGAAC CTGAAGCGAA AGCACAAGAG GAAGAAGAAT 
AAAATTACTC GTGACTTCAA ACCAGGAGAC AGGGTGGCAG TGGAGGTGGT GACCACCATG 
ACCTCAGCTG ACGTGATGTG GCAGGACGGC TCCGTGGAGT GCAACATCCG CTCCAATGAC 
CTCTTTCCTG TGCACCATCT GGACAACAAT GAGTTCTGCC CTGGAGACTT TGTGGTCGAC 
AAGCGAGTTC AGAGCTGTCC AGACCCTGCT GTGTATGGCG TGGTGCAGTC TGGGGACCAC 
ATCGGCCGCA CCTGCATGGT AAAGTGGTTC AAGCTGCGGC CGAGCGGGGA TGACGTGGAG 
CTGATTGGAG AAGAGGAAGA TGTGAGCGTG TATGACATTG CTGACCACCC TGACTTTCGG 
TTCCGCACGA CCGACATTGT CATTCGAATA GGCAACACTG AAGATGGGGC TGCACACAAT 
GAGGACGAAC CATCAGTGGG TCAGGTGGCC CGTGTGGACG TCAGCAGCAA GGTGGAGGTG 
GTGTGGGCCG ACAACTCGAA GACCATCATC CTGCCCCAGC ACTTGTACAA CATTGAATCC 
GAGATTGAGG AGTCAGACTA TGATTCAGTG GAAGGCAGCA CCAGTGGGGC ATCTTCAGAC 
GAGTGGGAGG ATGACAGTGA CAGCTGGGAG ACAGACAATG GACTGGTGGA AGATGAGCAC 
CCCAAAATAG AGGAGCCCCC CATCCTGCCC ACTGAGCAGC TGTCAGCACC CGAGGAGGAC 
AAAGGCGTAG TGATCAGTGA AGAGGCTGCC ACCGCTGCCA TCCAGGGGGC CGTGGCCATG 
CCTGCTCCCG TGGCAGGACT GATGGAGAAG GCTGGCAAGG ATGGGCCCCC CAAGAGCTTC 
CGAGAGCTGA AGGAGGCCAT CAAGATCCTG GAGAGCCTCA AAAACATGAC AGTGGAGCAG 
CTGCTCACTG GCTCTCCCAC TTCCCCCACT GTGGAGCCTG AGAAACCCAC CAGAGAGAAG 
AAGTTCTTGG ACGACATTAA GAAGCTGCAG GAGAACCTCA AGAAGACCCT GGACAACGTG 
GCCATCGCAG AGGAGGAGAA AATGGAGGCA GCACCTGACA CCGAGCGCAA GGAGGACAAG 
CCTGAAGGGC AGTCGCCAGT GAAGGCCGAG TGGCCCAGCG AGACCCCTGT GCTCTGCCAG 
CAGTGTGGTG GCAAGCCCGG TGTCACCTTC ACCAGCGCCA AAGGCGAGGT CTTCTCCGTG 
CTGGAGTTTG CACCTTCAAA TCACTCTTTT AAGAAAATTG AGTTCCAGCC TCCAGAAGCC 
AAGAAGTTCT TCAGCACGGT GCGGAAGGAG ATGGCACTGC TGGCTACCTC ACTGCCCGAT 
GGCATCATGG TCAAGACTTT TGAGGATAGA ATGGACCTCT TCTCAGCTCT AATCAAGGGC 
CCCACTCGAA CCCCCTACGA AGACGGCCTC TACCTGTTTG ACATCCAGCT CCCCAACATC 
TACCCAGCTG TGCCCCCCCA CTTTTGCTAC CTCTCCCAGT GCAGTGGCCG CCTGAACCCC 
AACCTGTATG ACAATGGGAA AGTGTGTGTC AGCCTCCTGG GCACCTGGAT TGGAAAGGGG 
ACAGAGAGGT GGACAAGTAA ATCCAGCCTA CTCCAGGTGC TCATCTCTAT CCAAGGTCTG 
ATCCTGGTGA ACGAACCTTA CTACAATGAA GCCGGCTTCG ACAGTGACCG GGGCCTGCAG 
GAAGGCTATG AGAACAGTCG CTGCTATAAT GAGATGGCAC TGATCCGTGT GGTACAGTCC 
ATGACACAGC TGGTTCGGCG GCCCCCCGAG GTCTTTGAGC AGGAGATCCG GCAGCACTTT 
AGCACCGGTG GCTGGCGGCT GGTGAACCGC ATCGAGTCCT GGTTGGAAAC CCACGCGCTA 
CTAGAGAGGG CCCAGGCGCT ACCCAATGGG GTCGCCAAGG ACAGCAGCTC TCCAGAGACA 
GCTGCTACGG CCGAGCTCTC AGACTCTGGC CGAGAAGAAC CTGAGGATGT CGGAATGGCC 
CCTGGAGAGG CCTCCCAGGG CTCAGACTCA GAGGGGGCTG TCCAGGGCCC GGCCTCCACT 
AGCAGGGACC ACACAGATCA GACTTCGGAG GTGGCACCTG ATGCATCCGT ACCACCCAGC 
GTCAAACCAA AGAAGCGGAG AAAGAGCTAT CGGAGCTTCT TGCCTGAGAA GAGCGGCTAC 
CCTGACATCG GCTTTCCCCT CTTCCCACTC TCTAAGGGTT TCATCAAAAG CATCAGGGGC 
GTCCTGACGC AGTTCCGGGC TGCGCTGCTA GAGGCAGGCA TGCCTGAAAG CACAGAGAAC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2o ( XM_003461440.3 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_003461488.1 Cavia porcellus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X2, mRNA.

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CloneID	ODo01434
Clone ID Related Accession (Same CDS sequence)	XM_003461440.4 , XM_003461440.3
Accession Version	XM_003461440.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176226.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 14, 2015 this sequence version replaced XM_003461440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTAGAG CCAGCTAGGA TTGCCTGGGA ATGTCCAGAA 
AAACACTGCG CCCCGGGGGA GGGCTCTATG GCCAAGAAGG TGAAGCGCCT TTTGAAGAAG 
CAGGTCGTGA GGATCATGTC TTGCTCCCCG GACACCCAGT GTGCCCGGGA CCATTCTATG 
GAGGACCCCG ACAAGAAAGG GGAAGCCAAG GCCAAGAGTG AAACAGGTTC TGCTAGCCCA 
GAGGAGCCAC CTGACAGGTC TGCCAGCCCA GTGGAGATGC AGGATGAGGG CCCTGAGGAG 
CCCCAGGAAA CCGGCCAGCC ACTGCCTCCC TTCCTGCTGA AGGAGGGTGG GGATGATGGG 
CTGCACTCAG CAGAGCAGGA TGCGGATGAC GAGGCTGCTG ATGACACTGA TGACACCAGC 
TCCGTGACCT CCTCTGCCAG CTCCACCACC TCCTCCCAGA GCGGCAGCGG CACAAGTCGC 
AAGAAGAGCA TCCCTTTGTC CATCAAGAAC CTGAAGCGAA AGCACAAGAG GAAGAAGAAT 
AAAATTACTC GTGACTTCAA ACCAGGAGAC AGGGTGGCAG TGGAGGTGGT GACCACCATG 
ACCTCAGCTG ACGTGATGTG GCAGGACGGC TCCGTGGAGT GCAACATCCG CTCCAATGAC 
CTCTTTCCTG TGCACCATCT GGACAACAAT GAGTTCTGCC CTGGAGACTT TGTGGTCGAC 
AAGCGAGTTC AGAGCTGTCC AGACCCTGCT GTGTATGGCG TGGTGCAGTC TGGGGACCAC 
ATCGGCCGCA CCTGCATGGT AAAGTGGTTC AAGCTGCGGC CGAGCGGGGA TGACGTGGAG 
CTGATTGGAG AAGAGGAAGA TGTGAGCGTG TATGACATTG CTGACCACCC TGACTTTCGG 
TTCCGCACGA CCGACATTGT CATTCGAATA GGCAACACTG AAGATGGGGC TGCACACAAT 
GAGGACGAAC CATCAGTGGG TCAGGTGGCC CGTGTGGACG TCAGCAGCAA GGTGGAGGTG 
GTGTGGGCCG ACAACTCGAA GACCATCATC CTGCCCCAGC ACTTGTACAA CATTGAATCC 
GAGATTGAGG AGTCAGACTA TGATTCAGTG GAAGGCAGCA CCAGTGGGGC ATCTTCAGAC 
GAGTGGGAGG ATGACAGTGA CAGCTGGGAG ACAGACAATG GACTGGTGGA AGATGAGCAC 
CCCAAAATAG AGGAGCCCCC CATCCTGCCC ACTGAGCAGC TGTCAGCACC CGAGGAGGAC 
AAAGGCGTAG TGATCAGTGA AGAGGCTGCC ACCGCTGCCA TCCAGGGGGC CGTGGCCATG 
CCTGCTCCCG TGGCAGGACT GATGGAGAAG GCTGGCAAGG ATGGGCCCCC CAAGAGCTTC 
CGAGAGCTGA AGGAGGCCAT CAAGATCCTG GAGAGCCTCA AAAACATGAC AGTGGAGCAG 
CTGCTCACTG GCTCTCCCAC TTCCCCCACT GTGGAGCCTG AGAAACCCAC CAGAGAGAAG 
AAGTTCTTGG ACGACATTAA GAAGCTGCAG GAGAACCTCA AGAAGACCCT GGACAACGTG 
GCCATCGCAG AGGAGGAGAA AATGGAGGCA GCACCTGACA CCGAGCGCAA GGAGGACAAG 
CCTGAAGGGC AGTCGCCAGT GAAGGCCGAG TGGCCCAGCG AGACCCCTGT GCTCTGCCAG 
CAGTGTGGTG GCAAGCCCGG TGTCACCTTC ACCAGCGCCA AAGGCGAGGT CTTCTCCGTG 
CTGGAGTTTG CACCTTCAAA TCACTCTTTT AAGAAAATTG AGTTCCAGCC TCCAGAAGCC 
AAGAAGTTCT TCAGCACGGT GCGGAAGGAG ATGGCACTGC TGGCTACCTC ACTGCCCGAT 
GGCATCATGG TCAAGACTTT TGAGGATAGA ATGGACCTCT TCTCAGCTCT AATCAAGGGC 
CCCACTCGAA CCCCCTACGA AGACGGCCTC TACCTGTTTG ACATCCAGCT CCCCAACATC 
TACCCAGCTG TGCCCCCCCA CTTTTGCTAC CTCTCCCAGT GCAGTGGCCG CCTGAACCCC 
AACCTGTATG ACAATGGGAA AGTGTGTGTC AGCCTCCTGG GCACCTGGAT TGGAAAGGGG 
ACAGAGAGGT GGACAAGTAA ATCCAGCCTA CTCCAGGTGC TCATCTCTAT CCAAGGTCTG 
ATCCTGGTGA ACGAACCTTA CTACAATGAA GCCGGCTTCG ACAGTGACCG GGGCCTGCAG 
GAAGGCTATG AGAACAGTCG CTGCTATAAT GAGATGGCAC TGATCCGTGT GGTACAGTCC 
ATGACACAGC TGGTTCGGCG GCCCCCCGAG GTCTTTGAGC AGGAGATCCG GCAGCACTTT 
AGCACCGGTG GCTGGCGGCT GGTGAACCGC ATCGAGTCCT GGTTGGAAAC CCACGCGCTA 
CTAGAGAGGG CCCAGGCGCT ACCCAATGGG GTCGCCAAGG ACAGCAGCTC TCCAGAGACA 
GCTGCTACGG CCGAGCTCTC AGACTCTGGC CGAGAAGAAC CTGAGGATGT CGGAATGGCC 
CCTGGAGAGG CCTCCCAGGG CTCAGACTCA GAGGGGGCTG TCCAGGGCCC GGCCTCCACT 
AGCAGGGACC ACACAGATCA GACTTCGGAG GTGGCACCTG ATGCATCCGT ACCACCCAGC 
GTCAAACCAA AGAAGCGGAG AAAGAGCTAT CGGAGCTTCT TGCCTGAGAA GAGCGGCTAC 
CCTGACATCG GCTTTCCCCT CTTCCCACTC TCTAAGGGTT TCATCAAAAG CATCAGGGGC 
GTCCTGACGC AGTTCCGGGC TGCGCTGCTA GAGGCAGGCA TGCCTGAAAG CACAGAGAAC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003461488.1
CDS	63..3968
Translation	MADPAASAPAAPAPPPAQAPAPEAASARAAAHSLTPAQASVPTPAPASDSASVPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDICPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPARIAWECPEKHCAPGEGSMAKKVKRLLKKQVVRIMSCSPDTQCARDHSMEDPDKKGEAKAKSETGSASPEEPPDRSASPVEMQDEGPEEPQETGQPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAAHNEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPILPTEQLSAPEEDKGVVISEEAATAAIQGAVAMPAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDTERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGVAKDSSSPETAATAELSDSGREEPEDVGMAPGEASQGSDSEGAVQGPASTSRDHTDQTSEVAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTENK

Target ORF information:

RefSeq Version	XM_003461440.3
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus ubiquitin-conjugating enzyme E2O (Ube2o), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003461440.3

ORF Insert Sequence:

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTAGAG CCAGCTAGGA TTGCCTGGGA ATGTCCAGAA 
AAACACTGCG CCCCGGGGGA GGGCTCTATG GCCAAGAAGG TGAAGCGCCT TTTGAAGAAG 
CAGGTCGTGA GGATCATGTC TTGCTCCCCG GACACCCAGT GTGCCCGGGA CCATTCTATG 
GAGGACCCCG ACAAGAAAGG GGAAGCCAAG GCCAAGAGTG AAACAGGTTC TGCTAGCCCA 
GAGGAGCCAC CTGACAGGTC TGCCAGCCCA GTGGAGATGC AGGATGAGGG CCCTGAGGAG 
CCCCAGGAAA CCGGCCAGCC ACTGCCTCCC TTCCTGCTGA AGGAGGGTGG GGATGATGGG 
CTGCACTCAG CAGAGCAGGA TGCGGATGAC GAGGCTGCTG ATGACACTGA TGACACCAGC 
TCCGTGACCT CCTCTGCCAG CTCCACCACC TCCTCCCAGA GCGGCAGCGG CACAAGTCGC 
AAGAAGAGCA TCCCTTTGTC CATCAAGAAC CTGAAGCGAA AGCACAAGAG GAAGAAGAAT 
AAAATTACTC GTGACTTCAA ACCAGGAGAC AGGGTGGCAG TGGAGGTGGT GACCACCATG 
ACCTCAGCTG ACGTGATGTG GCAGGACGGC TCCGTGGAGT GCAACATCCG CTCCAATGAC 
CTCTTTCCTG TGCACCATCT GGACAACAAT GAGTTCTGCC CTGGAGACTT TGTGGTCGAC 
AAGCGAGTTC AGAGCTGTCC AGACCCTGCT GTGTATGGCG TGGTGCAGTC TGGGGACCAC 
ATCGGCCGCA CCTGCATGGT AAAGTGGTTC AAGCTGCGGC CGAGCGGGGA TGACGTGGAG 
CTGATTGGAG AAGAGGAAGA TGTGAGCGTG TATGACATTG CTGACCACCC TGACTTTCGG 
TTCCGCACGA CCGACATTGT CATTCGAATA GGCAACACTG AAGATGGGGC TGCACACAAT 
GAGGACGAAC CATCAGTGGG TCAGGTGGCC CGTGTGGACG TCAGCAGCAA GGTGGAGGTG 
GTGTGGGCCG ACAACTCGAA GACCATCATC CTGCCCCAGC ACTTGTACAA CATTGAATCC 
GAGATTGAGG AGTCAGACTA TGATTCAGTG GAAGGCAGCA CCAGTGGGGC ATCTTCAGAC 
GAGTGGGAGG ATGACAGTGA CAGCTGGGAG ACAGACAATG GACTGGTGGA AGATGAGCAC 
CCCAAAATAG AGGAGCCCCC CATCCTGCCC ACTGAGCAGC TGTCAGCACC CGAGGAGGAC 
AAAGGCGTAG TGATCAGTGA AGAGGCTGCC ACCGCTGCCA TCCAGGGGGC CGTGGCCATG 
CCTGCTCCCG TGGCAGGACT GATGGAGAAG GCTGGCAAGG ATGGGCCCCC CAAGAGCTTC 
CGAGAGCTGA AGGAGGCCAT CAAGATCCTG GAGAGCCTCA AAAACATGAC AGTGGAGCAG 
CTGCTCACTG GCTCTCCCAC TTCCCCCACT GTGGAGCCTG AGAAACCCAC CAGAGAGAAG 
AAGTTCTTGG ACGACATTAA GAAGCTGCAG GAGAACCTCA AGAAGACCCT GGACAACGTG 
GCCATCGCAG AGGAGGAGAA AATGGAGGCA GCACCTGACA CCGAGCGCAA GGAGGACAAG 
CCTGAAGGGC AGTCGCCAGT GAAGGCCGAG TGGCCCAGCG AGACCCCTGT GCTCTGCCAG 
CAGTGTGGTG GCAAGCCCGG TGTCACCTTC ACCAGCGCCA AAGGCGAGGT CTTCTCCGTG 
CTGGAGTTTG CACCTTCAAA TCACTCTTTT AAGAAAATTG AGTTCCAGCC TCCAGAAGCC 
AAGAAGTTCT TCAGCACGGT GCGGAAGGAG ATGGCACTGC TGGCTACCTC ACTGCCCGAT 
GGCATCATGG TCAAGACTTT TGAGGATAGA ATGGACCTCT TCTCAGCTCT AATCAAGGGC 
CCCACTCGAA CCCCCTACGA AGACGGCCTC TACCTGTTTG ACATCCAGCT CCCCAACATC 
TACCCAGCTG TGCCCCCCCA CTTTTGCTAC CTCTCCCAGT GCAGTGGCCG CCTGAACCCC 
AACCTGTATG ACAATGGGAA AGTGTGTGTC AGCCTCCTGG GCACCTGGAT TGGAAAGGGG 
ACAGAGAGGT GGACAAGTAA ATCCAGCCTA CTCCAGGTGC TCATCTCTAT CCAAGGTCTG 
ATCCTGGTGA ACGAACCTTA CTACAATGAA GCCGGCTTCG ACAGTGACCG GGGCCTGCAG 
GAAGGCTATG AGAACAGTCG CTGCTATAAT GAGATGGCAC TGATCCGTGT GGTACAGTCC 
ATGACACAGC TGGTTCGGCG GCCCCCCGAG GTCTTTGAGC AGGAGATCCG GCAGCACTTT 
AGCACCGGTG GCTGGCGGCT GGTGAACCGC ATCGAGTCCT GGTTGGAAAC CCACGCGCTA 
CTAGAGAGGG CCCAGGCGCT ACCCAATGGG GTCGCCAAGG ACAGCAGCTC TCCAGAGACA 
GCTGCTACGG CCGAGCTCTC AGACTCTGGC CGAGAAGAAC CTGAGGATGT CGGAATGGCC 
CCTGGAGAGG CCTCCCAGGG CTCAGACTCA GAGGGGGCTG TCCAGGGCCC GGCCTCCACT 
AGCAGGGACC ACACAGATCA GACTTCGGAG GTGGCACCTG ATGCATCCGT ACCACCCAGC 
GTCAAACCAA AGAAGCGGAG AAAGAGCTAT CGGAGCTTCT TGCCTGAGAA GAGCGGCTAC 
CCTGACATCG GCTTTCCCCT CTTCCCACTC TCTAAGGGTT TCATCAAAAG CATCAGGGGC 
GTCCTGACGC AGTTCCGGGC TGCGCTGCTA GAGGCAGGCA TGCCTGAAAG CACAGAGAAC 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2o ( XM_023564973.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_023420741.1 Cavia porcellus ubiquitin conjugating enzyme E2 O (Ube2o), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODo41169
Clone ID Related Accession (Same CDS sequence)	XM_023564973.1
Accession Version	XM_023564973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176226.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTGAAG CGCCTTTTGA AGAAGCAGGT CGTGAGGATC 
ATGTCTTGCT CCCCGGACAC CCAGTGTGCC CGGGACCATT CTATGGAGGA CCCCGACAAG 
AAAGGGGAAG CCAAGGCCAA GAGTGAAACA GGTTCTGCTA GCCCAGAGGA GCCACCTGAC 
AGGTCTGCCA GCCCAGTGGA GATGCAGGAT GAGGGCCCTG AGGAGCCCCA GGAAACCGGC 
CAGCCACTGC CTCCCTTCCT GCTGAAGGAG GGTGGGGATG ATGGGCTGCA CTCAGCAGAG 
CAGGATGCGG ATGACGAGGC TGCTGATGAC ACTGATGACA CCAGCTCCGT GACCTCCTCT 
GCCAGCTCCA CCACCTCCTC CCAGAGCGGC AGCGGCACAA GTCGCAAGAA GAGCATCCCT 
TTGTCCATCA AGAACCTGAA GCGAAAGCAC AAGAGGAAGA AGAATAAAAT TACTCGTGAC 
TTCAAACCAG GAGACAGGGT GGCAGTGGAG GTGGTGACCA CCATGACCTC AGCTGACGTG 
ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC ATCCGCTCCA ATGACCTCTT TCCTGTGCAC 
CATCTGGACA ACAATGAGTT CTGCCCTGGA GACTTTGTGG TCGACAAGCG AGTTCAGAGC 
TGTCCAGACC CTGCTGTGTA TGGCGTGGTG CAGTCTGGGG ACCACATCGG CCGCACCTGC 
ATGGTAAAGT GGTTCAAGCT GCGGCCGAGC GGGGATGACG TGGAGCTGAT TGGAGAAGAG 
GAAGATGTGA GCGTGTATGA CATTGCTGAC CACCCTGACT TTCGGTTCCG CACGACCGAC 
ATTGTCATTC GAATAGGCAA CACTGAAGAT GGGGCTGCAC ACAATGAGGA CGAACAGCCA 
TCAGTGGGTC AGGTGGCCCG TGTGGACGTC AGCAGCAAGG TGGAGGTGGT GTGGGCCGAC 
AACTCGAAGA CCATCATCCT GCCCCAGCAC TTGTACAACA TTGAATCCGA GATTGAGGAG 
TCAGACTATG ATTCAGTGGA AGGCAGCACC AGTGGGGCAT CTTCAGACGA GTGGGAGGAT 
GACAGTGACA GCTGGGAGAC AGACAATGGA CTGGTGGAAG ATGAGCACCC CAAAATAGAG 
GAGCCCCCCA TCCTGCCCAC TGAGCAGCTG TCAGCACCCG AGGAGGACAA AGGCGTAGTG 
ATCAGTGAAG AGGCTGCCAC CGCTGCCATC CAGGGGGCCG TGGCCATGCC TGCTCCCGTG 
GCAGGACTGA TGGAGAAGGC TGGCAAGGAT GGGCCCCCCA AGAGCTTCCG AGAGCTGAAG 
GAGGCCATCA AGATCCTGGA GAGCCTCAAA AACATGACAG TGGAGCAGCT GCTCACTGGC 
TCTCCCACTT CCCCCACTGT GGAGCCTGAG AAACCCACCA GAGAGAAGAA GTTCTTGGAC 
GACATTAAGA AGCTGCAGGA GAACCTCAAG AAGACCCTGG ACAACGTGGC CATCGCAGAG 
GAGGAGAAAA TGGAGGCAGC ACCTGACACC GAGCGCAAGG AGGACAAGCC TGAAGGGCAG 
TCGCCAGTGA AGGCCGAGTG GCCCAGCGAG ACCCCTGTGC TCTGCCAGCA GTGTGGTGGC 
AAGCCCGGTG TCACCTTCAC CAGCGCCAAA GGCGAGGTCT TCTCCGTGCT GGAGTTTGCA 
CCTTCAAATC ACTCTTTTAA GAAAATTGAG TTCCAGCCTC CAGAAGCCAA GAAGTTCTTC 
AGCACGGTGC GGAAGGAGAT GGCACTGCTG GCTACCTCAC TGCCCGATGG CATCATGGTC 
AAGACTTTTG AGGATAGAAT GGACCTCTTC TCAGCTCTAA TCAAGGGCCC CACTCGAACC 
CCCTACGAAG ACGGCCTCTA CCTGTTTGAC ATCCAGCTCC CCAACATCTA CCCAGCTGTG 
CCCCCCCACT TTTGCTACCT CTCCCAGTGC AGTGGCCGCC TGAACCCCAA CCTGTATGAC 
AATGGGAAAG TGTGTGTCAG CCTCCTGGGC ACCTGGATTG GAAAGGGGAC AGAGAGGTGG 
ACAAGTAAAT CCAGCCTACT CCAGGTGCTC ATCTCTATCC AAGGTCTGAT CCTGGTGAAC 
GAACCTTACT ACAATGAAGC CGGCTTCGAC AGTGACCGGG GCCTGCAGGA AGGCTATGAG 
AACAGTCGCT GCTATAATGA GATGGCACTG ATCCGTGTGG TACAGTCCAT GACACAGCTG 
GTTCGGCGGC CCCCCGAGGT CTTTGAGCAG GAGATCCGGC AGCACTTTAG CACCGGTGGC 
TGGCGGCTGG TGAACCGCAT CGAGTCCTGG TTGGAAACCC ACGCGCTACT AGAGAGGGCC 
CAGGCGCTAC CCAATGGGGT CGCCAAGGAC AGCAGCTCTC CAGAGACAGC TGCTACGGCC 
GAGCTCTCAG ACTCTGGCCG AGAAGAACCT GAGGATGTCG GAATGGCCCC TGGAGAGGCC 
TCCCAGGGCT CAGACTCAGA GGGGGCTGTC CAGGGCCCGG CCTCCACTAG CAGGGACCAC 
ACAGATCAGA CTTCGGAGGT GGCACCTGAT GCATCCGTAC CACCCAGCGT CAAACCAAAG 
AAGCGGAGAA AGAGCTATCG GAGCTTCTTG CCTGAGAAGA GCGGCTACCC TGACATCGGC 
TTTCCCCTCT TCCCACTCTC TAAGGGTTTC ATCAAAAGCA TCAGGGGCGT CCTGACGCAG 
TTCCGGGCTG CGCTGCTAGA GGCAGGCATG CCTGAAAGCA CAGAGAACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023420741.1
CDS	69..3902
Translation	MADPAASAPAAPAPPPAQAPAPEAASARAAAHSLTPAQASVPTPAPASDSASVPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDICPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQVVRIMSCSPDTQCARDHSMEDPDKKGEAKAKSETGSASPEEPPDRSASPVEMQDEGPEEPQETGQPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAAHNEDEQPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPILPTEQLSAPEEDKGVVISEEAATAAIQGAVAMPAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAAPDTERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGVAKDSSSPETAATAELSDSGREEPEDVGMAPGEASQGSDSEGAVQGPASTSRDHTDQTSEVAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTENK

Target ORF information:

RefSeq Version	XM_023564973.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus ubiquitin conjugating enzyme E2 O (Ube2o), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023564973.1

ORF Insert Sequence:

ATGGCGGACC CCGCAGCCTC CGCGCCCGCG GCTCCCGCTC CCCCTCCCGC CCAGGCCCCA 
GCTCCAGAGG CCGCCTCAGC CCGGGCCGCA GCCCACTCCC TGACCCCAGC CCAGGCCTCA 
GTGCCAACGC CGGCGCCAGC CTCGGACTCG GCCTCGGTGC CGTCCTCAGA CTCCGGCCCC 
GAAGCCGGCT CGCAGCGCCT GCTCTTCTCC CACGACCTGG TTTCGGGTCG CTACCGCGGC 
TCGGTGCACT TCGGGCTGGT GCGCCTAATC CACGGCGAGG ACTCAGACTC CGAGGGAGAA 
GAAGAGGGCC GCGGCAGCTC AGGCTGCTCG GAGGCCGGGG GCGCGGGCCA CGAGGAGGGC 
CGGGCCAGCC CGTTGCGCCG CGGCTATGTG CGCGTCCAGT GGTACCCCGA GGGCGTCAAG 
CAGCACGTGA AGGAGACCAA GCTGAAACTA GAGGACCGTT CGGTGGTGCC CCGAGATGTG 
GTCCGGCACA TGCGCTCCAC CGACAGTCAG TGTGGCACAG TGATAGACGT CAACATCGAC 
TGCGCTGTCA AGCTCATCGG CACCAACTGC ATCATCTACC CCGTCAACAG CAAGGATCTG 
CAGCACATCT GGCCCTTCAT GTATGGGGAC TACATTGCCT ATGACTGCTG GCTGGGAAAG 
GTCTATGACT TGAAGAACCA GATCATCCTC AAGCTGTCCA ATGGCGCCAG GTGCTCCATG 
AACACAGAAG ATGGTGCCAA GCTGTACGAC ATCTGCCCAC ATGTCAGTGA CTCGGGTCTC 
TTCTTTGATG ATTCCTATGG CTTCTACCCG GGCCAGGTGC TCATCGGCCC CGCCAAGATC 
TTCTCCAGTG TCCAGTGGTT GTCAGGGGTC AAGCCCGTAC TCAGCACCAA GAGCAAGTTC 
CGGGTGGTGG TGGAGGAGGT TCAGGTCGTG GAGCTGAAAG TCACATGGAT TACCAAGAGT 
TTCTGTCCTG GGGGCACAGA CAGCGTCAGC CCCCCACCCT CTGTTATCAC TCAGGAAAAT 
CTAGGCAGGG TGAAGCGTCT CGGATGCTTT GACCATGCTC AGCGGCAGCT GGGGGAACGC 
TGTCTGTATG TCTTCCCAGC CAAGGTGAAG CGCCTTTTGA AGAAGCAGGT CGTGAGGATC 
ATGTCTTGCT CCCCGGACAC CCAGTGTGCC CGGGACCATT CTATGGAGGA CCCCGACAAG 
AAAGGGGAAG CCAAGGCCAA GAGTGAAACA GGTTCTGCTA GCCCAGAGGA GCCACCTGAC 
AGGTCTGCCA GCCCAGTGGA GATGCAGGAT GAGGGCCCTG AGGAGCCCCA GGAAACCGGC 
CAGCCACTGC CTCCCTTCCT GCTGAAGGAG GGTGGGGATG ATGGGCTGCA CTCAGCAGAG 
CAGGATGCGG ATGACGAGGC TGCTGATGAC ACTGATGACA CCAGCTCCGT GACCTCCTCT 
GCCAGCTCCA CCACCTCCTC CCAGAGCGGC AGCGGCACAA GTCGCAAGAA GAGCATCCCT 
TTGTCCATCA AGAACCTGAA GCGAAAGCAC AAGAGGAAGA AGAATAAAAT TACTCGTGAC 
TTCAAACCAG GAGACAGGGT GGCAGTGGAG GTGGTGACCA CCATGACCTC AGCTGACGTG 
ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC ATCCGCTCCA ATGACCTCTT TCCTGTGCAC 
CATCTGGACA ACAATGAGTT CTGCCCTGGA GACTTTGTGG TCGACAAGCG AGTTCAGAGC 
TGTCCAGACC CTGCTGTGTA TGGCGTGGTG CAGTCTGGGG ACCACATCGG CCGCACCTGC 
ATGGTAAAGT GGTTCAAGCT GCGGCCGAGC GGGGATGACG TGGAGCTGAT TGGAGAAGAG 
GAAGATGTGA GCGTGTATGA CATTGCTGAC CACCCTGACT TTCGGTTCCG CACGACCGAC 
ATTGTCATTC GAATAGGCAA CACTGAAGAT GGGGCTGCAC ACAATGAGGA CGAACAGCCA 
TCAGTGGGTC AGGTGGCCCG TGTGGACGTC AGCAGCAAGG TGGAGGTGGT GTGGGCCGAC 
AACTCGAAGA CCATCATCCT GCCCCAGCAC TTGTACAACA TTGAATCCGA GATTGAGGAG 
TCAGACTATG ATTCAGTGGA AGGCAGCACC AGTGGGGCAT CTTCAGACGA GTGGGAGGAT 
GACAGTGACA GCTGGGAGAC AGACAATGGA CTGGTGGAAG ATGAGCACCC CAAAATAGAG 
GAGCCCCCCA TCCTGCCCAC TGAGCAGCTG TCAGCACCCG AGGAGGACAA AGGCGTAGTG 
ATCAGTGAAG AGGCTGCCAC CGCTGCCATC CAGGGGGCCG TGGCCATGCC TGCTCCCGTG 
GCAGGACTGA TGGAGAAGGC TGGCAAGGAT GGGCCCCCCA AGAGCTTCCG AGAGCTGAAG 
GAGGCCATCA AGATCCTGGA GAGCCTCAAA AACATGACAG TGGAGCAGCT GCTCACTGGC 
TCTCCCACTT CCCCCACTGT GGAGCCTGAG AAACCCACCA GAGAGAAGAA GTTCTTGGAC 
GACATTAAGA AGCTGCAGGA GAACCTCAAG AAGACCCTGG ACAACGTGGC CATCGCAGAG 
GAGGAGAAAA TGGAGGCAGC ACCTGACACC GAGCGCAAGG AGGACAAGCC TGAAGGGCAG 
TCGCCAGTGA AGGCCGAGTG GCCCAGCGAG ACCCCTGTGC TCTGCCAGCA GTGTGGTGGC 
AAGCCCGGTG TCACCTTCAC CAGCGCCAAA GGCGAGGTCT TCTCCGTGCT GGAGTTTGCA 
CCTTCAAATC ACTCTTTTAA GAAAATTGAG TTCCAGCCTC CAGAAGCCAA GAAGTTCTTC 
AGCACGGTGC GGAAGGAGAT GGCACTGCTG GCTACCTCAC TGCCCGATGG CATCATGGTC 
AAGACTTTTG AGGATAGAAT GGACCTCTTC TCAGCTCTAA TCAAGGGCCC CACTCGAACC 
CCCTACGAAG ACGGCCTCTA CCTGTTTGAC ATCCAGCTCC CCAACATCTA CCCAGCTGTG 
CCCCCCCACT TTTGCTACCT CTCCCAGTGC AGTGGCCGCC TGAACCCCAA CCTGTATGAC 
AATGGGAAAG TGTGTGTCAG CCTCCTGGGC ACCTGGATTG GAAAGGGGAC AGAGAGGTGG 
ACAAGTAAAT CCAGCCTACT CCAGGTGCTC ATCTCTATCC AAGGTCTGAT CCTGGTGAAC 
GAACCTTACT ACAATGAAGC CGGCTTCGAC AGTGACCGGG GCCTGCAGGA AGGCTATGAG 
AACAGTCGCT GCTATAATGA GATGGCACTG ATCCGTGTGG TACAGTCCAT GACACAGCTG 
GTTCGGCGGC CCCCCGAGGT CTTTGAGCAG GAGATCCGGC AGCACTTTAG CACCGGTGGC 
TGGCGGCTGG TGAACCGCAT CGAGTCCTGG TTGGAAACCC ACGCGCTACT AGAGAGGGCC 
CAGGCGCTAC CCAATGGGGT CGCCAAGGAC AGCAGCTCTC CAGAGACAGC TGCTACGGCC 
GAGCTCTCAG ACTCTGGCCG AGAAGAACCT GAGGATGTCG GAATGGCCCC TGGAGAGGCC 
TCCCAGGGCT CAGACTCAGA GGGGGCTGTC CAGGGCCCGG CCTCCACTAG CAGGGACCAC 
ACAGATCAGA CTTCGGAGGT GGCACCTGAT GCATCCGTAC CACCCAGCGT CAAACCAAAG 
AAGCGGAGAA AGAGCTATCG GAGCTTCTTG CCTGAGAAGA GCGGCTACCC TGACATCGGC 
TTTCCCCTCT TCCCACTCTC TAAGGGTTTC ATCAAAAGCA TCAGGGGCGT CCTGACGCAG 
TTCCGGGCTG CGCTGCTAGA GGCAGGCATG CCTGAAAGCA CAGAGAACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Ube2o cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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