MIER2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIER2
Entrez Gene ID	612437
Full Name	MIER family member 2
General protein information	Preferred Names MIER family member 2 Names mesoderm induction early response protein 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022407333.1	XP_022263041.1	mesoderm induction early response protein 2 isoform X2
XM_022407335.1	XP_022263043.1	mesoderm induction early response protein 2 isoform X3
XM_005633174.1	XP_005633231.1	mesoderm induction early response protein 2 isoform X1
XM_005633175.1	XP_005633232.1	mesoderm induction early response protein 2 isoform X2
XM_005633174.2	XP_005633231.1	mesoderm induction early response protein 2 isoform X1
XM_005633175.2	XP_005633232.1	mesoderm induction early response protein 2 isoform X3
XM_022407334.1	XP_022263042.1	mesoderm induction early response protein 2 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MIER2	NP_060020.1
Gallus gallus (chicken)	MIER2	XP_004948841.1
Macaca mulatta (Rhesus monkey)	MIER2	XP_001090898.2
Canis lupus familiaris (dog)	MIER2	XP_005633231.1
Rattus norvegicus (Norway rat)	Mier2	NP_001102207.1
Danio rerio (zebrafish)	LOC564723	XP_693146.5
Mus musculus (house mouse)	Mier2	NP_081698.2
Pan troglodytes (chimpanzee)	LOC455527	XP_003316013.1
Bos taurus (cattle)	MIER2	NP_001092496.1

Gene Ontology

The following MIER2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIER2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe22570	XM_005633174.2 Latest version!	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe22571	XM_005633175.2 Latest version!	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe22571	XM_022407335.1 Latest version!	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe295779	XM_022407334.1 Latest version!	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe295779	XM_022407333.1 Latest version!	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OCe22570	XM_005633174.1	Canis lupus familiaris mesoderm induction early response 1, family member 2 (MIER2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCe22571	XM_005633175.1	Canis lupus familiaris mesoderm induction early response 1, family member 2 (MIER2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIER2 ( XM_005633174.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633231.1 Canis lupus familiaris mesoderm induction early response 1, family member 2 (MIER2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe22570
Clone ID Related Accession (Same CDS sequence)	XM_005633174.1 , XM_005633174.2
Accession Version	XM_005633174.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	mesoderm induction early response protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCTCCTCGCT GGAGAGGCAG AGCCCTGGCG TGGCCTCCTG CCTCACTCAC 
AGCCTGTGCC CTGGGGAGCC CAGCTTGCAG ACAGCAGCAG TGGTGTCCAT GGGCTCTGCA 
GACCATCAGT TCAACCTCGT AGAGATCCTG TCCCAGAACT ACGGCATCCG GGAGGAGTGT 
GAGCAGGCCA CGGGAGACAC GGAGAAGCCC GAGGGGGAGC TGGAGAAGGA CTTCATCTCC 
CAGAGCAGCG ACATGCCCCT CGACGAGCTG CTCGCCCTCT ACGGCTATGA GGCCTCAGAC 
CCCATCTCGG AGCAGGGCAG CGAGAGCAGC GACACCACGG CCAACCTCCC GGACATGACG 
CTGGACAAGG AACAAATAGC AAAGGATTTG CTCTCAGGGG AAGAAGAGGA AGAGACACAG 
TCGTCGGCCG ATGACCTTAC CCCGTCCGTG ACCTCCCACG AGGCCTCTGA CCTGTTTCCT 
AACCGGAGTG CATCTTGCTT CCTGGCCGAC GCAGGCAAAG AGCCTGGCTC CTCCGCTTCA 
TCCGACACGG AGGAGGACCC CCTTCCTGCC AACAAGTGTA AGAAGGAGAT CATGGTGGGG 
CCTCAGTTCC AAGCCGACCT CAGCGGCCTG CAGTTGGAAC GCCACGGTGA GAAACTCTAC 
GAGAACGAGG ACCAGCTGCT CTGGGACCCC AACGTCCTCC CTGAGAGGGA AGTAGAGGAG 
TTCCTGTACC GGGCGGTGAA GCGGAGGTGG CACGAGATGG CTGGGTCGCA GCTTCCGGAG 
GGAGAGGTGG TGAAAGACAG CGAGCAGGCG CTTTACGAGC TGGTGAAGTG CAACTTCAAC 
GCGGAGGAAG CCCTGCGGAG GCTGCGATTC AACGTGAAGG TGATCCGAGA TGGGTTTTGC 
GCGTGGAGCG AGGAGGAGTG CAGGAACTTT GAGCACGGCT TCCGAGTGCA CGGGAAGAAC 
TTCCATCTGA TCCAGGCCAA CAAGGTGCGC ACGCGGTCTG TGGGCGAGTG CGTGGAGTAC 
TACTACCTGT GGAAGAAGTC TGAGCGCTAC GACTACTTCT CGCAGCAGAC GCGGCTGGGC 
CGGAGGAAAT ACGGCCCGTC TGGAGCCACG GACGCAGACC AGGACCTGGA CGGCAGCGAC 
CCCGATGGCG CAGGCCGCCC CCGCTCCTCG CCGCCTCTGC CCCCTGCTGC CGACGGCCTG 
GCCTCTGAGC AGGACCCGCT GGCGCGGATG CACACGGAGC CGCTGAGCGT AGACAGCGCG 
GCCGGCAGTG TTGGTGAGCC CGGGGAGAGC TCCGACAGCC TCCCGTCCTC GGAGCCGGGG 
CCTTGTCCCT TCCAGCAGCT GGACAGCCCT CCAGCCGCGT CCCTGCCCAG GCGGCCCCCA 
GCCCTGGCCG AGCCCGCCTT CTACCCCCCG GCCACGGCCA CCCCGGAGCC CAGCAGCAGC 
CCGAGACTGG CTGTGGACTT GGCCCTGCCG GGGGCCCTCC CCGAGGAGCT GCCCCTCATC 
TCCAGCCACG CGGACATGAA CGGAGAGCCC GAGGAGGCTG TGGCCCCGGC ACAGGTGGCC 
CTGTCGGTCA CCGAGTTTGG ACTCATCGGC ATTGGGGACG TGAATCCCTT CCTGGCCGCC 
CACCCAGCGT GCCCGGCCCC CGGGCTGCAC TCGGAGCCCC TGTCACACTG TAACGTGATG 
ACCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633231.1
CDS	39..1727
Translation	MAEASSLERQSPGVASCLTHSLCPGEPSLQTAAVVSMGSADHQFNLVEILSQNYGIREECEQATGDTEKPEGELEKDFISQSSDMPLDELLALYGYEASDPISEQGSESSDTTANLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSASCFLADAGKEPGSSASSDTEEDPLPANKCKKEIMVGPQFQADLSGLQLERHGEKLYENEDQLLWDPNVLPEREVEEFLYRAVKRRWHEMAGSQLPEGEVVKDSEQALYELVKCNFNAEEALRRLRFNVKVIRDGFCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFSQQTRLGRRKYGPSGATDADQDLDGSDPDGAGRPRSSPPLPPAADGLASEQDPLARMHTEPLSVDSAAGSVGEPGESSDSLPSSEPGPCPFQQLDSPPAASLPRRPPALAEPAFYPPATATPEPSSSPRLAVDLALPGALPEELPLISSHADMNGEPEEAVAPAQVALSVTEFGLIGIGDVNPFLAAHPACPAPGLHSEPLSHCNVMTC

Target ORF information:

RefSeq Version	XM_005633174.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mesoderm induction early response 1, family member 2 (MIER2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633174.1

ORF Insert Sequence:

ATGGCGGAGG CCTCCTCGCT GGAGAGGCAG AGCCCTGGCG TGGCCTCCTG CCTCACTCAC 
AGCCTGTGCC CTGGGGAGCC CAGCTTGCAG ACAGCAGCAG TGGTGTCCAT GGGCTCTGCA 
GACCATCAGT TCAACCTCGT AGAGATCCTG TCCCAGAACT ACGGCATCCG GGAGGAGTGT 
GAGCAGGCCA CGGGAGACAC GGAGAAGCCC GAGGGGGAGC TGGAGAAGGA CTTCATCTCC 
CAGAGCAGCG ACATGCCCCT CGACGAGCTG CTCGCCCTCT ACGGCTATGA GGCCTCAGAC 
CCCATCTCGG AGCAGGGCAG CGAGAGCAGC GACACCACGG CCAACCTCCC GGACATGACG 
CTGGACAAGG AACAAATAGC AAAGGATTTG CTCTCAGGGG AAGAAGAGGA AGAGACACAG 
TCGTCGGCCG ATGACCTTAC CCCGTCCGTG ACCTCCCACG AGGCCTCTGA CCTGTTTCCT 
AACCGGAGTG CATCTTGCTT CCTGGCCGAC GCAGGCAAAG AGCCTGGCTC CTCCGCTTCA 
TCCGACACGG AGGAGGACCC CCTTCCTGCC AACAAGTGTA AGAAGGAGAT CATGGTGGGG 
CCTCAGTTCC AAGCCGACCT CAGCGGCCTG CAGTTGGAAC GCCACGGTGA GAAACTCTAC 
GAGAACGAGG ACCAGCTGCT CTGGGACCCC AACGTCCTCC CTGAGAGGGA AGTAGAGGAG 
TTCCTGTACC GGGCGGTGAA GCGGAGGTGG CACGAGATGG CTGGGTCGCA GCTTCCGGAG 
GGAGAGGTGG TGAAAGACAG CGAGCAGGCG CTTTACGAGC TGGTGAAGTG CAACTTCAAC 
GCGGAGGAAG CCCTGCGGAG GCTGCGATTC AACGTGAAGG TGATCCGAGA TGGGTTTTGC 
GCGTGGAGCG AGGAGGAGTG CAGGAACTTT GAGCACGGCT TCCGAGTGCA CGGGAAGAAC 
TTCCATCTGA TCCAGGCCAA CAAGGTGCGC ACGCGGTCTG TGGGCGAGTG CGTGGAGTAC 
TACTACCTGT GGAAGAAGTC TGAGCGCTAC GACTACTTCT CGCAGCAGAC GCGGCTGGGC 
CGGAGGAAAT ACGGCCCGTC TGGAGCCACG GACGCAGACC AGGACCTGGA CGGCAGCGAC 
CCCGATGGCG CAGGCCGCCC CCGCTCCTCG CCGCCTCTGC CCCCTGCTGC CGACGGCCTG 
GCCTCTGAGC AGGACCCGCT GGCGCGGATG CACACGGAGC CGCTGAGCGT AGACAGCGCG 
GCCGGCAGTG TTGGTGAGCC CGGGGAGAGC TCCGACAGCC TCCCGTCCTC GGAGCCGGGG 
CCTTGTCCCT TCCAGCAGCT GGACAGCCCT CCAGCCGCGT CCCTGCCCAG GCGGCCCCCA 
GCCCTGGCCG AGCCCGCCTT CTACCCCCCG GCCACGGCCA CCCCGGAGCC CAGCAGCAGC 
CCGAGACTGG CTGTGGACTT GGCCCTGCCG GGGGCCCTCC CCGAGGAGCT GCCCCTCATC 
TCCAGCCACG CGGACATGAA CGGAGAGCCC GAGGAGGCTG TGGCCCCGGC ACAGGTGGCC 
CTGTCGGTCA CCGAGTTTGG ACTCATCGGC ATTGGGGACG TGAATCCCTT CCTGGCCGCC 
CACCCAGCGT GCCCGGCCCC CGGGCTGCAC TCGGAGCCCC TGTCACACTG TAACGTGATG 
ACCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIER2 ( XM_005633174.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633231.1 Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe22570
Clone ID Related Accession (Same CDS sequence)	XM_005633174.1 , XM_005633174.2
Accession Version	XM_005633174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	mesoderm induction early response protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCTCCTCGCT GGAGAGGCAG AGCCCTGGCG TGGCCTCCTG CCTCACTCAC 
AGCCTGTGCC CTGGGGAGCC CAGCTTGCAG ACAGCAGCAG TGGTGTCCAT GGGCTCTGCA 
GACCATCAGT TCAACCTCGT AGAGATCCTG TCCCAGAACT ACGGCATCCG GGAGGAGTGT 
GAGCAGGCCA CGGGAGACAC GGAGAAGCCC GAGGGGGAGC TGGAGAAGGA CTTCATCTCC 
CAGAGCAGCG ACATGCCCCT CGACGAGCTG CTCGCCCTCT ACGGCTATGA GGCCTCAGAC 
CCCATCTCGG AGCAGGGCAG CGAGAGCAGC GACACCACGG CCAACCTCCC GGACATGACG 
CTGGACAAGG AACAAATAGC AAAGGATTTG CTCTCAGGGG AAGAAGAGGA AGAGACACAG 
TCGTCGGCCG ATGACCTTAC CCCGTCCGTG ACCTCCCACG AGGCCTCTGA CCTGTTTCCT 
AACCGGAGTG CATCTTGCTT CCTGGCCGAC GCAGGCAAAG AGCCTGGCTC CTCCGCTTCA 
TCCGACACGG AGGAGGACCC CCTTCCTGCC AACAAGTGTA AGAAGGAGAT CATGGTGGGG 
CCTCAGTTCC AAGCCGACCT CAGCGGCCTG CAGTTGGAAC GCCACGGTGA GAAACTCTAC 
GAGAACGAGG ACCAGCTGCT CTGGGACCCC AACGTCCTCC CTGAGAGGGA AGTAGAGGAG 
TTCCTGTACC GGGCGGTGAA GCGGAGGTGG CACGAGATGG CTGGGTCGCA GCTTCCGGAG 
GGAGAGGTGG TGAAAGACAG CGAGCAGGCG CTTTACGAGC TGGTGAAGTG CAACTTCAAC 
GCGGAGGAAG CCCTGCGGAG GCTGCGATTC AACGTGAAGG TGATCCGAGA TGGGTTTTGC 
GCGTGGAGCG AGGAGGAGTG CAGGAACTTT GAGCACGGCT TCCGAGTGCA CGGGAAGAAC 
TTCCATCTGA TCCAGGCCAA CAAGGTGCGC ACGCGGTCTG TGGGCGAGTG CGTGGAGTAC 
TACTACCTGT GGAAGAAGTC TGAGCGCTAC GACTACTTCT CGCAGCAGAC GCGGCTGGGC 
CGGAGGAAAT ACGGCCCGTC TGGAGCCACG GACGCAGACC AGGACCTGGA CGGCAGCGAC 
CCCGATGGCG CAGGCCGCCC CCGCTCCTCG CCGCCTCTGC CCCCTGCTGC CGACGGCCTG 
GCCTCTGAGC AGGACCCGCT GGCGCGGATG CACACGGAGC CGCTGAGCGT AGACAGCGCG 
GCCGGCAGTG TTGGTGAGCC CGGGGAGAGC TCCGACAGCC TCCCGTCCTC GGAGCCGGGG 
CCTTGTCCCT TCCAGCAGCT GGACAGCCCT CCAGCCGCGT CCCTGCCCAG GCGGCCCCCA 
GCCCTGGCCG AGCCCGCCTT CTACCCCCCG GCCACGGCCA CCCCGGAGCC CAGCAGCAGC 
CCGAGACTGG CTGTGGACTT GGCCCTGCCG GGGGCCCTCC CCGAGGAGCT GCCCCTCATC 
TCCAGCCACG CGGACATGAA CGGAGAGCCC GAGGAGGCTG TGGCCCCGGC ACAGGTGGCC 
CTGTCGGTCA CCGAGTTTGG ACTCATCGGC ATTGGGGACG TGAATCCCTT CCTGGCCGCC 
CACCCAGCGT GCCCGGCCCC CGGGCTGCAC TCGGAGCCCC TGTCACACTG TAACGTGATG 
ACCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633231.1
CDS	44..1732
Translation	MAEASSLERQSPGVASCLTHSLCPGEPSLQTAAVVSMGSADHQFNLVEILSQNYGIREECEQATGDTEKPEGELEKDFISQSSDMPLDELLALYGYEASDPISEQGSESSDTTANLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSASCFLADAGKEPGSSASSDTEEDPLPANKCKKEIMVGPQFQADLSGLQLERHGEKLYENEDQLLWDPNVLPEREVEEFLYRAVKRRWHEMAGSQLPEGEVVKDSEQALYELVKCNFNAEEALRRLRFNVKVIRDGFCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFSQQTRLGRRKYGPSGATDADQDLDGSDPDGAGRPRSSPPLPPAADGLASEQDPLARMHTEPLSVDSAAGSVGEPGESSDSLPSSEPGPCPFQQLDSPPAASLPRRPPALAEPAFYPPATATPEPSSSPRLAVDLALPGALPEELPLISSHADMNGEPEEAVAPAQVALSVTEFGLIGIGDVNPFLAAHPACPAPGLHSEPLSHCNVMTC

Target ORF information:

RefSeq Version	XM_005633174.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633174.2

ORF Insert Sequence:

ATGGCGGAGG CCTCCTCGCT GGAGAGGCAG AGCCCTGGCG TGGCCTCCTG CCTCACTCAC 
AGCCTGTGCC CTGGGGAGCC CAGCTTGCAG ACAGCAGCAG TGGTGTCCAT GGGCTCTGCA 
GACCATCAGT TCAACCTCGT AGAGATCCTG TCCCAGAACT ACGGCATCCG GGAGGAGTGT 
GAGCAGGCCA CGGGAGACAC GGAGAAGCCC GAGGGGGAGC TGGAGAAGGA CTTCATCTCC 
CAGAGCAGCG ACATGCCCCT CGACGAGCTG CTCGCCCTCT ACGGCTATGA GGCCTCAGAC 
CCCATCTCGG AGCAGGGCAG CGAGAGCAGC GACACCACGG CCAACCTCCC GGACATGACG 
CTGGACAAGG AACAAATAGC AAAGGATTTG CTCTCAGGGG AAGAAGAGGA AGAGACACAG 
TCGTCGGCCG ATGACCTTAC CCCGTCCGTG ACCTCCCACG AGGCCTCTGA CCTGTTTCCT 
AACCGGAGTG CATCTTGCTT CCTGGCCGAC GCAGGCAAAG AGCCTGGCTC CTCCGCTTCA 
TCCGACACGG AGGAGGACCC CCTTCCTGCC AACAAGTGTA AGAAGGAGAT CATGGTGGGG 
CCTCAGTTCC AAGCCGACCT CAGCGGCCTG CAGTTGGAAC GCCACGGTGA GAAACTCTAC 
GAGAACGAGG ACCAGCTGCT CTGGGACCCC AACGTCCTCC CTGAGAGGGA AGTAGAGGAG 
TTCCTGTACC GGGCGGTGAA GCGGAGGTGG CACGAGATGG CTGGGTCGCA GCTTCCGGAG 
GGAGAGGTGG TGAAAGACAG CGAGCAGGCG CTTTACGAGC TGGTGAAGTG CAACTTCAAC 
GCGGAGGAAG CCCTGCGGAG GCTGCGATTC AACGTGAAGG TGATCCGAGA TGGGTTTTGC 
GCGTGGAGCG AGGAGGAGTG CAGGAACTTT GAGCACGGCT TCCGAGTGCA CGGGAAGAAC 
TTCCATCTGA TCCAGGCCAA CAAGGTGCGC ACGCGGTCTG TGGGCGAGTG CGTGGAGTAC 
TACTACCTGT GGAAGAAGTC TGAGCGCTAC GACTACTTCT CGCAGCAGAC GCGGCTGGGC 
CGGAGGAAAT ACGGCCCGTC TGGAGCCACG GACGCAGACC AGGACCTGGA CGGCAGCGAC 
CCCGATGGCG CAGGCCGCCC CCGCTCCTCG CCGCCTCTGC CCCCTGCTGC CGACGGCCTG 
GCCTCTGAGC AGGACCCGCT GGCGCGGATG CACACGGAGC CGCTGAGCGT AGACAGCGCG 
GCCGGCAGTG TTGGTGAGCC CGGGGAGAGC TCCGACAGCC TCCCGTCCTC GGAGCCGGGG 
CCTTGTCCCT TCCAGCAGCT GGACAGCCCT CCAGCCGCGT CCCTGCCCAG GCGGCCCCCA 
GCCCTGGCCG AGCCCGCCTT CTACCCCCCG GCCACGGCCA CCCCGGAGCC CAGCAGCAGC 
CCGAGACTGG CTGTGGACTT GGCCCTGCCG GGGGCCCTCC CCGAGGAGCT GCCCCTCATC 
TCCAGCCACG CGGACATGAA CGGAGAGCCC GAGGAGGCTG TGGCCCCGGC ACAGGTGGCC 
CTGTCGGTCA CCGAGTTTGG ACTCATCGGC ATTGGGGACG TGAATCCCTT CCTGGCCGCC 
CACCCAGCGT GCCCGGCCCC CGGGCTGCAC TCGGAGCCCC TGTCACACTG TAACGTGATG 
ACCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIER2 ( XM_022407335.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022263043.1 Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe22571
Clone ID Related Accession (Same CDS sequence)	XM_022407335.1 , XM_005633175.1 , XM_005633175.2
Accession Version	XM_022407335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	mesoderm induction early response protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG CAGACCATCA GTTCAACCTC GTAGAGATCC TGTCCCAGAA CTACGGCATC 
CGGGAGGAGT GTGAGCAGGC CACGGGAGAC ACGGAGAAGC CCGAGGGGGA GCTGGAGAAG 
GACTTCATCT CCCAGAGCAG CGACATGCCC CTCGACGAGC TGCTCGCCCT CTACGGCTAT 
GAGGCCTCAG ACCCCATCTC GGAGCAGGGC AGCGAGAGCA GCGACACCAC GGCCAACCTC 
CCGGACATGA CGCTGGACAA GGAACAAATA GCAAAGGATT TGCTCTCAGG GGAAGAAGAG 
GAAGAGACAC AGTCGTCGGC CGATGACCTT ACCCCGTCCG TGACCTCCCA CGAGGCCTCT 
GACCTGTTTC CTAACCGGAG TGCATCTTGC TTCCTGGCCG ACGCAGGCAA AGAGCCTGGC 
TCCTCCGCTT CATCCGACAC GGAGGAGGAC CCCCTTCCTG CCAACAAGTG TAAGAAGGAG 
ATCATGGTGG GGCCTCAGTT CCAAGCCGAC CTCAGCGGCC TGCAGTTGGA ACGCCACGGT 
GAGAAACTCT ACGAGAACGA GGACCAGCTG CTCTGGGACC CCAACGTCCT CCCTGAGAGG 
GAAGTAGAGG AGTTCCTGTA CCGGGCGGTG AAGCGGAGGT GGCACGAGAT GGCTGGGTCG 
CAGCTTCCGG AGGGAGAGGT GGTGAAAGAC AGCGAGCAGG CGCTTTACGA GCTGGTGAAG 
TGCAACTTCA ACGCGGAGGA AGCCCTGCGG AGGCTGCGAT TCAACGTGAA GGTGATCCGA 
GATGGGTTTT GCGCGTGGAG CGAGGAGGAG TGCAGGAACT TTGAGCACGG CTTCCGAGTG 
CACGGGAAGA ACTTCCATCT GATCCAGGCC AACAAGGTGC GCACGCGGTC TGTGGGCGAG 
TGCGTGGAGT ACTACTACCT GTGGAAGAAG TCTGAGCGCT ACGACTACTT CTCGCAGCAG 
ACGCGGCTGG GCCGGAGGAA ATACGGCCCG TCTGGAGCCA CGGACGCAGA CCAGGACCTG 
GACGGCAGCG ACCCCGATGG CGCAGGCCGC CCCCGCTCCT CGCCGCCTCT GCCCCCTGCT 
GCCGACGGCC TGGCCTCTGA GCAGGACCCG CTGGCGCGGA TGCACACGGA GCCGCTGAGC 
GTAGACAGCG CGGCCGGCAG TGTTGGTGAG CCCGGGGAGA GCTCCGACAG CCTCCCGTCC 
TCGGAGCCGG GGCCTTGTCC CTTCCAGCAG CTGGACAGCC CTCCAGCCGC GTCCCTGCCC 
AGGCGGCCCC CAGCCCTGGC CGAGCCCGCC TTCTACCCCC CGGCCACGGC CACCCCGGAG 
CCCAGCAGCA GCCCGAGACT GGCTGTGGAC TTGGCCCTGC CGGGGGCCCT CCCCGAGGAG 
CTGCCCCTCA TCTCCAGCCA CGCGGACATG AACGGAGAGC CCGAGGAGGC TGTGGCCCCG 
GCACAGGTGG CCCTGTCGGT CACCGAGTTT GGACTCATCG GCATTGGGGA CGTGAATCCC 
TTCCTGGCCG CCCACCCAGC GTGCCCGGCC CCCGGGCTGC ACTCGGAGCC CCTGTCACAC 
TGTAACGTGA TGACCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022263043.1
CDS	223..1803
Translation	MGSADHQFNLVEILSQNYGIREECEQATGDTEKPEGELEKDFISQSSDMPLDELLALYGYEASDPISEQGSESSDTTANLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSASCFLADAGKEPGSSASSDTEEDPLPANKCKKEIMVGPQFQADLSGLQLERHGEKLYENEDQLLWDPNVLPEREVEEFLYRAVKRRWHEMAGSQLPEGEVVKDSEQALYELVKCNFNAEEALRRLRFNVKVIRDGFCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFSQQTRLGRRKYGPSGATDADQDLDGSDPDGAGRPRSSPPLPPAADGLASEQDPLARMHTEPLSVDSAAGSVGEPGESSDSLPSSEPGPCPFQQLDSPPAASLPRRPPALAEPAFYPPATATPEPSSSPRLAVDLALPGALPEELPLISSHADMNGEPEEAVAPAQVALSVTEFGLIGIGDVNPFLAAHPACPAPGLHSEPLSHCNVMTC

Target ORF information:

RefSeq Version	XM_022407335.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407335.1

ORF Insert Sequence:

ATGGGCTCTG CAGACCATCA GTTCAACCTC GTAGAGATCC TGTCCCAGAA CTACGGCATC 
CGGGAGGAGT GTGAGCAGGC CACGGGAGAC ACGGAGAAGC CCGAGGGGGA GCTGGAGAAG 
GACTTCATCT CCCAGAGCAG CGACATGCCC CTCGACGAGC TGCTCGCCCT CTACGGCTAT 
GAGGCCTCAG ACCCCATCTC GGAGCAGGGC AGCGAGAGCA GCGACACCAC GGCCAACCTC 
CCGGACATGA CGCTGGACAA GGAACAAATA GCAAAGGATT TGCTCTCAGG GGAAGAAGAG 
GAAGAGACAC AGTCGTCGGC CGATGACCTT ACCCCGTCCG TGACCTCCCA CGAGGCCTCT 
GACCTGTTTC CTAACCGGAG TGCATCTTGC TTCCTGGCCG ACGCAGGCAA AGAGCCTGGC 
TCCTCCGCTT CATCCGACAC GGAGGAGGAC CCCCTTCCTG CCAACAAGTG TAAGAAGGAG 
ATCATGGTGG GGCCTCAGTT CCAAGCCGAC CTCAGCGGCC TGCAGTTGGA ACGCCACGGT 
GAGAAACTCT ACGAGAACGA GGACCAGCTG CTCTGGGACC CCAACGTCCT CCCTGAGAGG 
GAAGTAGAGG AGTTCCTGTA CCGGGCGGTG AAGCGGAGGT GGCACGAGAT GGCTGGGTCG 
CAGCTTCCGG AGGGAGAGGT GGTGAAAGAC AGCGAGCAGG CGCTTTACGA GCTGGTGAAG 
TGCAACTTCA ACGCGGAGGA AGCCCTGCGG AGGCTGCGAT TCAACGTGAA GGTGATCCGA 
GATGGGTTTT GCGCGTGGAG CGAGGAGGAG TGCAGGAACT TTGAGCACGG CTTCCGAGTG 
CACGGGAAGA ACTTCCATCT GATCCAGGCC AACAAGGTGC GCACGCGGTC TGTGGGCGAG 
TGCGTGGAGT ACTACTACCT GTGGAAGAAG TCTGAGCGCT ACGACTACTT CTCGCAGCAG 
ACGCGGCTGG GCCGGAGGAA ATACGGCCCG TCTGGAGCCA CGGACGCAGA CCAGGACCTG 
GACGGCAGCG ACCCCGATGG CGCAGGCCGC CCCCGCTCCT CGCCGCCTCT GCCCCCTGCT 
GCCGACGGCC TGGCCTCTGA GCAGGACCCG CTGGCGCGGA TGCACACGGA GCCGCTGAGC 
GTAGACAGCG CGGCCGGCAG TGTTGGTGAG CCCGGGGAGA GCTCCGACAG CCTCCCGTCC 
TCGGAGCCGG GGCCTTGTCC CTTCCAGCAG CTGGACAGCC CTCCAGCCGC GTCCCTGCCC 
AGGCGGCCCC CAGCCCTGGC CGAGCCCGCC TTCTACCCCC CGGCCACGGC CACCCCGGAG 
CCCAGCAGCA GCCCGAGACT GGCTGTGGAC TTGGCCCTGC CGGGGGCCCT CCCCGAGGAG 
CTGCCCCTCA TCTCCAGCCA CGCGGACATG AACGGAGAGC CCGAGGAGGC TGTGGCCCCG 
GCACAGGTGG CCCTGTCGGT CACCGAGTTT GGACTCATCG GCATTGGGGA CGTGAATCCC 
TTCCTGGCCG CCCACCCAGC GTGCCCGGCC CCCGGGCTGC ACTCGGAGCC CCTGTCACAC 
TGTAACGTGA TGACCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIER2 ( XM_005633175.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633232.1 Canis lupus familiaris mesoderm induction early response 1, family member 2 (MIER2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe22571
Clone ID Related Accession (Same CDS sequence)	XM_022407335.1 , XM_005633175.1 , XM_005633175.2
Accession Version	XM_005633175.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	mesoderm induction early response protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG CAGACCATCA GTTCAACCTC GTAGAGATCC TGTCCCAGAA CTACGGCATC 
CGGGAGGAGT GTGAGCAGGC CACGGGAGAC ACGGAGAAGC CCGAGGGGGA GCTGGAGAAG 
GACTTCATCT CCCAGAGCAG CGACATGCCC CTCGACGAGC TGCTCGCCCT CTACGGCTAT 
GAGGCCTCAG ACCCCATCTC GGAGCAGGGC AGCGAGAGCA GCGACACCAC GGCCAACCTC 
CCGGACATGA CGCTGGACAA GGAACAAATA GCAAAGGATT TGCTCTCAGG GGAAGAAGAG 
GAAGAGACAC AGTCGTCGGC CGATGACCTT ACCCCGTCCG TGACCTCCCA CGAGGCCTCT 
GACCTGTTTC CTAACCGGAG TGCATCTTGC TTCCTGGCCG ACGCAGGCAA AGAGCCTGGC 
TCCTCCGCTT CATCCGACAC GGAGGAGGAC CCCCTTCCTG CCAACAAGTG TAAGAAGGAG 
ATCATGGTGG GGCCTCAGTT CCAAGCCGAC CTCAGCGGCC TGCAGTTGGA ACGCCACGGT 
GAGAAACTCT ACGAGAACGA GGACCAGCTG CTCTGGGACC CCAACGTCCT CCCTGAGAGG 
GAAGTAGAGG AGTTCCTGTA CCGGGCGGTG AAGCGGAGGT GGCACGAGAT GGCTGGGTCG 
CAGCTTCCGG AGGGAGAGGT GGTGAAAGAC AGCGAGCAGG CGCTTTACGA GCTGGTGAAG 
TGCAACTTCA ACGCGGAGGA AGCCCTGCGG AGGCTGCGAT TCAACGTGAA GGTGATCCGA 
GATGGGTTTT GCGCGTGGAG CGAGGAGGAG TGCAGGAACT TTGAGCACGG CTTCCGAGTG 
CACGGGAAGA ACTTCCATCT GATCCAGGCC AACAAGGTGC GCACGCGGTC TGTGGGCGAG 
TGCGTGGAGT ACTACTACCT GTGGAAGAAG TCTGAGCGCT ACGACTACTT CTCGCAGCAG 
ACGCGGCTGG GCCGGAGGAA ATACGGCCCG TCTGGAGCCA CGGACGCAGA CCAGGACCTG 
GACGGCAGCG ACCCCGATGG CGCAGGCCGC CCCCGCTCCT CGCCGCCTCT GCCCCCTGCT 
GCCGACGGCC TGGCCTCTGA GCAGGACCCG CTGGCGCGGA TGCACACGGA GCCGCTGAGC 
GTAGACAGCG CGGCCGGCAG TGTTGGTGAG CCCGGGGAGA GCTCCGACAG CCTCCCGTCC 
TCGGAGCCGG GGCCTTGTCC CTTCCAGCAG CTGGACAGCC CTCCAGCCGC GTCCCTGCCC 
AGGCGGCCCC CAGCCCTGGC CGAGCCCGCC TTCTACCCCC CGGCCACGGC CACCCCGGAG 
CCCAGCAGCA GCCCGAGACT GGCTGTGGAC TTGGCCCTGC CGGGGGCCCT CCCCGAGGAG 
CTGCCCCTCA TCTCCAGCCA CGCGGACATG AACGGAGAGC CCGAGGAGGC TGTGGCCCCG 
GCACAGGTGG CCCTGTCGGT CACCGAGTTT GGACTCATCG GCATTGGGGA CGTGAATCCC 
TTCCTGGCCG CCCACCCAGC GTGCCCGGCC CCCGGGCTGC ACTCGGAGCC CCTGTCACAC 
TGTAACGTGA TGACCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633232.1
CDS	257..1837
Translation	MGSADHQFNLVEILSQNYGIREECEQATGDTEKPEGELEKDFISQSSDMPLDELLALYGYEASDPISEQGSESSDTTANLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSASCFLADAGKEPGSSASSDTEEDPLPANKCKKEIMVGPQFQADLSGLQLERHGEKLYENEDQLLWDPNVLPEREVEEFLYRAVKRRWHEMAGSQLPEGEVVKDSEQALYELVKCNFNAEEALRRLRFNVKVIRDGFCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFSQQTRLGRRKYGPSGATDADQDLDGSDPDGAGRPRSSPPLPPAADGLASEQDPLARMHTEPLSVDSAAGSVGEPGESSDSLPSSEPGPCPFQQLDSPPAASLPRRPPALAEPAFYPPATATPEPSSSPRLAVDLALPGALPEELPLISSHADMNGEPEEAVAPAQVALSVTEFGLIGIGDVNPFLAAHPACPAPGLHSEPLSHCNVMTC

Target ORF information:

RefSeq Version	XM_005633175.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mesoderm induction early response 1, family member 2 (MIER2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633175.1

ORF Insert Sequence:

ATGGGCTCTG CAGACCATCA GTTCAACCTC GTAGAGATCC TGTCCCAGAA CTACGGCATC 
CGGGAGGAGT GTGAGCAGGC CACGGGAGAC ACGGAGAAGC CCGAGGGGGA GCTGGAGAAG 
GACTTCATCT CCCAGAGCAG CGACATGCCC CTCGACGAGC TGCTCGCCCT CTACGGCTAT 
GAGGCCTCAG ACCCCATCTC GGAGCAGGGC AGCGAGAGCA GCGACACCAC GGCCAACCTC 
CCGGACATGA CGCTGGACAA GGAACAAATA GCAAAGGATT TGCTCTCAGG GGAAGAAGAG 
GAAGAGACAC AGTCGTCGGC CGATGACCTT ACCCCGTCCG TGACCTCCCA CGAGGCCTCT 
GACCTGTTTC CTAACCGGAG TGCATCTTGC TTCCTGGCCG ACGCAGGCAA AGAGCCTGGC 
TCCTCCGCTT CATCCGACAC GGAGGAGGAC CCCCTTCCTG CCAACAAGTG TAAGAAGGAG 
ATCATGGTGG GGCCTCAGTT CCAAGCCGAC CTCAGCGGCC TGCAGTTGGA ACGCCACGGT 
GAGAAACTCT ACGAGAACGA GGACCAGCTG CTCTGGGACC CCAACGTCCT CCCTGAGAGG 
GAAGTAGAGG AGTTCCTGTA CCGGGCGGTG AAGCGGAGGT GGCACGAGAT GGCTGGGTCG 
CAGCTTCCGG AGGGAGAGGT GGTGAAAGAC AGCGAGCAGG CGCTTTACGA GCTGGTGAAG 
TGCAACTTCA ACGCGGAGGA AGCCCTGCGG AGGCTGCGAT TCAACGTGAA GGTGATCCGA 
GATGGGTTTT GCGCGTGGAG CGAGGAGGAG TGCAGGAACT TTGAGCACGG CTTCCGAGTG 
CACGGGAAGA ACTTCCATCT GATCCAGGCC AACAAGGTGC GCACGCGGTC TGTGGGCGAG 
TGCGTGGAGT ACTACTACCT GTGGAAGAAG TCTGAGCGCT ACGACTACTT CTCGCAGCAG 
ACGCGGCTGG GCCGGAGGAA ATACGGCCCG TCTGGAGCCA CGGACGCAGA CCAGGACCTG 
GACGGCAGCG ACCCCGATGG CGCAGGCCGC CCCCGCTCCT CGCCGCCTCT GCCCCCTGCT 
GCCGACGGCC TGGCCTCTGA GCAGGACCCG CTGGCGCGGA TGCACACGGA GCCGCTGAGC 
GTAGACAGCG CGGCCGGCAG TGTTGGTGAG CCCGGGGAGA GCTCCGACAG CCTCCCGTCC 
TCGGAGCCGG GGCCTTGTCC CTTCCAGCAG CTGGACAGCC CTCCAGCCGC GTCCCTGCCC 
AGGCGGCCCC CAGCCCTGGC CGAGCCCGCC TTCTACCCCC CGGCCACGGC CACCCCGGAG 
CCCAGCAGCA GCCCGAGACT GGCTGTGGAC TTGGCCCTGC CGGGGGCCCT CCCCGAGGAG 
CTGCCCCTCA TCTCCAGCCA CGCGGACATG AACGGAGAGC CCGAGGAGGC TGTGGCCCCG 
GCACAGGTGG CCCTGTCGGT CACCGAGTTT GGACTCATCG GCATTGGGGA CGTGAATCCC 
TTCCTGGCCG CCCACCCAGC GTGCCCGGCC CCCGGGCTGC ACTCGGAGCC CCTGTCACAC 
TGTAACGTGA TGACCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIER2 ( XM_005633175.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633232.1 Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe22571
Clone ID Related Accession (Same CDS sequence)	XM_022407335.1 , XM_005633175.1 , XM_005633175.2
Accession Version	XM_005633175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	mesoderm induction early response protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG CAGACCATCA GTTCAACCTC GTAGAGATCC TGTCCCAGAA CTACGGCATC 
CGGGAGGAGT GTGAGCAGGC CACGGGAGAC ACGGAGAAGC CCGAGGGGGA GCTGGAGAAG 
GACTTCATCT CCCAGAGCAG CGACATGCCC CTCGACGAGC TGCTCGCCCT CTACGGCTAT 
GAGGCCTCAG ACCCCATCTC GGAGCAGGGC AGCGAGAGCA GCGACACCAC GGCCAACCTC 
CCGGACATGA CGCTGGACAA GGAACAAATA GCAAAGGATT TGCTCTCAGG GGAAGAAGAG 
GAAGAGACAC AGTCGTCGGC CGATGACCTT ACCCCGTCCG TGACCTCCCA CGAGGCCTCT 
GACCTGTTTC CTAACCGGAG TGCATCTTGC TTCCTGGCCG ACGCAGGCAA AGAGCCTGGC 
TCCTCCGCTT CATCCGACAC GGAGGAGGAC CCCCTTCCTG CCAACAAGTG TAAGAAGGAG 
ATCATGGTGG GGCCTCAGTT CCAAGCCGAC CTCAGCGGCC TGCAGTTGGA ACGCCACGGT 
GAGAAACTCT ACGAGAACGA GGACCAGCTG CTCTGGGACC CCAACGTCCT CCCTGAGAGG 
GAAGTAGAGG AGTTCCTGTA CCGGGCGGTG AAGCGGAGGT GGCACGAGAT GGCTGGGTCG 
CAGCTTCCGG AGGGAGAGGT GGTGAAAGAC AGCGAGCAGG CGCTTTACGA GCTGGTGAAG 
TGCAACTTCA ACGCGGAGGA AGCCCTGCGG AGGCTGCGAT TCAACGTGAA GGTGATCCGA 
GATGGGTTTT GCGCGTGGAG CGAGGAGGAG TGCAGGAACT TTGAGCACGG CTTCCGAGTG 
CACGGGAAGA ACTTCCATCT GATCCAGGCC AACAAGGTGC GCACGCGGTC TGTGGGCGAG 
TGCGTGGAGT ACTACTACCT GTGGAAGAAG TCTGAGCGCT ACGACTACTT CTCGCAGCAG 
ACGCGGCTGG GCCGGAGGAA ATACGGCCCG TCTGGAGCCA CGGACGCAGA CCAGGACCTG 
GACGGCAGCG ACCCCGATGG CGCAGGCCGC CCCCGCTCCT CGCCGCCTCT GCCCCCTGCT 
GCCGACGGCC TGGCCTCTGA GCAGGACCCG CTGGCGCGGA TGCACACGGA GCCGCTGAGC 
GTAGACAGCG CGGCCGGCAG TGTTGGTGAG CCCGGGGAGA GCTCCGACAG CCTCCCGTCC 
TCGGAGCCGG GGCCTTGTCC CTTCCAGCAG CTGGACAGCC CTCCAGCCGC GTCCCTGCCC 
AGGCGGCCCC CAGCCCTGGC CGAGCCCGCC TTCTACCCCC CGGCCACGGC CACCCCGGAG 
CCCAGCAGCA GCCCGAGACT GGCTGTGGAC TTGGCCCTGC CGGGGGCCCT CCCCGAGGAG 
CTGCCCCTCA TCTCCAGCCA CGCGGACATG AACGGAGAGC CCGAGGAGGC TGTGGCCCCG 
GCACAGGTGG CCCTGTCGGT CACCGAGTTT GGACTCATCG GCATTGGGGA CGTGAATCCC 
TTCCTGGCCG CCCACCCAGC GTGCCCGGCC CCCGGGCTGC ACTCGGAGCC CCTGTCACAC 
TGTAACGTGA TGACCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633232.1
CDS	264..1844
Translation	MGSADHQFNLVEILSQNYGIREECEQATGDTEKPEGELEKDFISQSSDMPLDELLALYGYEASDPISEQGSESSDTTANLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSASCFLADAGKEPGSSASSDTEEDPLPANKCKKEIMVGPQFQADLSGLQLERHGEKLYENEDQLLWDPNVLPEREVEEFLYRAVKRRWHEMAGSQLPEGEVVKDSEQALYELVKCNFNAEEALRRLRFNVKVIRDGFCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFSQQTRLGRRKYGPSGATDADQDLDGSDPDGAGRPRSSPPLPPAADGLASEQDPLARMHTEPLSVDSAAGSVGEPGESSDSLPSSEPGPCPFQQLDSPPAASLPRRPPALAEPAFYPPATATPEPSSSPRLAVDLALPGALPEELPLISSHADMNGEPEEAVAPAQVALSVTEFGLIGIGDVNPFLAAHPACPAPGLHSEPLSHCNVMTC

Target ORF information:

RefSeq Version	XM_005633175.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633175.2

ORF Insert Sequence:

ATGGGCTCTG CAGACCATCA GTTCAACCTC GTAGAGATCC TGTCCCAGAA CTACGGCATC 
CGGGAGGAGT GTGAGCAGGC CACGGGAGAC ACGGAGAAGC CCGAGGGGGA GCTGGAGAAG 
GACTTCATCT CCCAGAGCAG CGACATGCCC CTCGACGAGC TGCTCGCCCT CTACGGCTAT 
GAGGCCTCAG ACCCCATCTC GGAGCAGGGC AGCGAGAGCA GCGACACCAC GGCCAACCTC 
CCGGACATGA CGCTGGACAA GGAACAAATA GCAAAGGATT TGCTCTCAGG GGAAGAAGAG 
GAAGAGACAC AGTCGTCGGC CGATGACCTT ACCCCGTCCG TGACCTCCCA CGAGGCCTCT 
GACCTGTTTC CTAACCGGAG TGCATCTTGC TTCCTGGCCG ACGCAGGCAA AGAGCCTGGC 
TCCTCCGCTT CATCCGACAC GGAGGAGGAC CCCCTTCCTG CCAACAAGTG TAAGAAGGAG 
ATCATGGTGG GGCCTCAGTT CCAAGCCGAC CTCAGCGGCC TGCAGTTGGA ACGCCACGGT 
GAGAAACTCT ACGAGAACGA GGACCAGCTG CTCTGGGACC CCAACGTCCT CCCTGAGAGG 
GAAGTAGAGG AGTTCCTGTA CCGGGCGGTG AAGCGGAGGT GGCACGAGAT GGCTGGGTCG 
CAGCTTCCGG AGGGAGAGGT GGTGAAAGAC AGCGAGCAGG CGCTTTACGA GCTGGTGAAG 
TGCAACTTCA ACGCGGAGGA AGCCCTGCGG AGGCTGCGAT TCAACGTGAA GGTGATCCGA 
GATGGGTTTT GCGCGTGGAG CGAGGAGGAG TGCAGGAACT TTGAGCACGG CTTCCGAGTG 
CACGGGAAGA ACTTCCATCT GATCCAGGCC AACAAGGTGC GCACGCGGTC TGTGGGCGAG 
TGCGTGGAGT ACTACTACCT GTGGAAGAAG TCTGAGCGCT ACGACTACTT CTCGCAGCAG 
ACGCGGCTGG GCCGGAGGAA ATACGGCCCG TCTGGAGCCA CGGACGCAGA CCAGGACCTG 
GACGGCAGCG ACCCCGATGG CGCAGGCCGC CCCCGCTCCT CGCCGCCTCT GCCCCCTGCT 
GCCGACGGCC TGGCCTCTGA GCAGGACCCG CTGGCGCGGA TGCACACGGA GCCGCTGAGC 
GTAGACAGCG CGGCCGGCAG TGTTGGTGAG CCCGGGGAGA GCTCCGACAG CCTCCCGTCC 
TCGGAGCCGG GGCCTTGTCC CTTCCAGCAG CTGGACAGCC CTCCAGCCGC GTCCCTGCCC 
AGGCGGCCCC CAGCCCTGGC CGAGCCCGCC TTCTACCCCC CGGCCACGGC CACCCCGGAG 
CCCAGCAGCA GCCCGAGACT GGCTGTGGAC TTGGCCCTGC CGGGGGCCCT CCCCGAGGAG 
CTGCCCCTCA TCTCCAGCCA CGCGGACATG AACGGAGAGC CCGAGGAGGC TGTGGCCCCG 
GCACAGGTGG CCCTGTCGGT CACCGAGTTT GGACTCATCG GCATTGGGGA CGTGAATCCC 
TTCCTGGCCG CCCACCCAGC GTGCCCGGCC CCCGGGCTGC ACTCGGAGCC CCTGTCACAC 
TGTAACGTGA TGACCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIER2 ( XM_022407333.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022263041.1 Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe295779
Clone ID Related Accession (Same CDS sequence)	XM_022407333.1 , XM_022407334.1
Accession Version	XM_022407333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	mesoderm induction early response protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCTCCTCGCT GGAGAGGCAG AGCCCTGGCG TGGCCTCCTG CCTCACTCAC 
AGCCTGTGCC CTGGGGAGCC CAGCTTGCAG ACAGCAGCAG TGGTGTCCAT GGGCTCTGCA 
GACCATCAGT TCAACCTCGT AGAGATCCTG TCCCAGAACT ACGGCATCCG GGAGGAGTGT 
GAGCAGGCCA CGGGAGACAC GGAGAAGCCC GAGGGGGAGC TGGAGAAGGA CTTCATCTCC 
CAGAGCAGCG ACATGCCCCT CGACGAGCTG CTCGCCCTCT ACGGCTATGA GGCCTCAGAC 
CCCATCTCGG AGCAGGGCAG CGAGAGCAGC GACACCACGG CCAACCTCCC GGACATGACG 
CTGGACAAGG AACAAATAGC AAAGGATTTG CTCTCAGGGG AAGAAGAGGA AGAGACACAG 
TCGTCGGCCG ATGACCTTAC CCCGTCCGTG ACCTCCCACG AGGCCTCTGA CCTGTTTCCT 
AACCGGAGTG CATCTTGCTT CCTGGCCGAC GCAGGCAAAG AGCCTGGCTC CTCCGCTTCA 
TCCGACACGG AGGAGGACCC CCTTCCTGCC AACAAGTGTA AGAAGGAGAT CATGGTGGGG 
CCTCAGTTCC AAGCCGACCT CAGCGGCCTG CAGTTGGAAC GCCACGGTGA GAAACTCTAC 
GAGAACGAGG ACCAGCTGCT CTGGGACCCC AACGTCCTCC CTGAGAGGGA AGTAGAGGAG 
TTCCTGTACC GGGCGGTGAA GCGGAGGTGG CACGAGATGG CTGGGTCGCA GCTTCCGGAG 
GGAGAGGTGG TGAAAGACAG CGAGCAGGCG CTTTACGAGC TGGTGAAGTG CAACTTCAAC 
GCGGAGGAAG CCCTGCGGAG GCTGCGATTC AACGTGAAGG TGATCCGAGA TGGGTTTTGC 
GCGTGGAGCG AGGAGGAGTG CAGGAACTTT GAGCACGGCT TCCGAGTGCA CGGGAAGAAC 
TTCCATCTGA TCCAGGCCAA CAAGGTGCGC ACGCGGTCTG TGGGCGAGTG CGTGGAGTAC 
TACTACCTGT GGAAGAAGTC TGAGCGCTAC GACTACTTCT CGCAGCAGAC GCGGCTGGGC 
CGGAGGAAAT ACGGCCCGTC TGGAGCCACG GACGCAGACC AGGACCTGGA CGGCAGCGAC 
CCCGATGGCG CAGGCCGCCC CCGCTCCTCG CCGCCTCTGC CCCCTGCTGC CGACGGCCTG 
GCCTCTGAGC AGGACCCGCT GGCGCGGATG CACACGGAGC CGCTGAGCGT AGACAGCGCG 
GCCGGCAGTG TTGGTGAGCC CGGGGAGAGC TCCGACAGCC TCCCGTCCTC GGAGCCGGGG 
CCTTGTCCCT TCCAGCAGCT GGACAGCCCT CCAGCCGCGT CCCTGCCCAG GCGGCCCCCA 
GCCCTGGCCG AGCCCGCCTT CTACCCCCCG GCCACGGCCA CCCCGGAGCC CAGCAGCAGC 
CCGAGACTGG CTGTGGACTT GGCCCTGCCG GGGGCCCTCC CCGAGGAGCT GCCCCTCATC 
TCCAGCCACG CGGACATGAA CGGAGAGCCC GAGGAGGCTG TGGCCCCGGC ACAGGTGGCC 
CTGTCGGTCA CCGAGTTTGG ACTCATCGGC ATTGGGGACG TGAATCCCTT CCTGGCCGCC 
CACCCAGCGT GCCCGGCCCC CGGGCTGCAC TCGGAGCCCC TGTCACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022263041.1
CDS	44..1714
Translation	MAEASSLERQSPGVASCLTHSLCPGEPSLQTAAVVSMGSADHQFNLVEILSQNYGIREECEQATGDTEKPEGELEKDFISQSSDMPLDELLALYGYEASDPISEQGSESSDTTANLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSASCFLADAGKEPGSSASSDTEEDPLPANKCKKEIMVGPQFQADLSGLQLERHGEKLYENEDQLLWDPNVLPEREVEEFLYRAVKRRWHEMAGSQLPEGEVVKDSEQALYELVKCNFNAEEALRRLRFNVKVIRDGFCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFSQQTRLGRRKYGPSGATDADQDLDGSDPDGAGRPRSSPPLPPAADGLASEQDPLARMHTEPLSVDSAAGSVGEPGESSDSLPSSEPGPCPFQQLDSPPAASLPRRPPALAEPAFYPPATATPEPSSSPRLAVDLALPGALPEELPLISSHADMNGEPEEAVAPAQVALSVTEFGLIGIGDVNPFLAAHPACPAPGLHSEPLSQ

Target ORF information:

RefSeq Version	XM_022407333.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407333.1

ORF Insert Sequence:

ATGGCGGAGG CCTCCTCGCT GGAGAGGCAG AGCCCTGGCG TGGCCTCCTG CCTCACTCAC 
AGCCTGTGCC CTGGGGAGCC CAGCTTGCAG ACAGCAGCAG TGGTGTCCAT GGGCTCTGCA 
GACCATCAGT TCAACCTCGT AGAGATCCTG TCCCAGAACT ACGGCATCCG GGAGGAGTGT 
GAGCAGGCCA CGGGAGACAC GGAGAAGCCC GAGGGGGAGC TGGAGAAGGA CTTCATCTCC 
CAGAGCAGCG ACATGCCCCT CGACGAGCTG CTCGCCCTCT ACGGCTATGA GGCCTCAGAC 
CCCATCTCGG AGCAGGGCAG CGAGAGCAGC GACACCACGG CCAACCTCCC GGACATGACG 
CTGGACAAGG AACAAATAGC AAAGGATTTG CTCTCAGGGG AAGAAGAGGA AGAGACACAG 
TCGTCGGCCG ATGACCTTAC CCCGTCCGTG ACCTCCCACG AGGCCTCTGA CCTGTTTCCT 
AACCGGAGTG CATCTTGCTT CCTGGCCGAC GCAGGCAAAG AGCCTGGCTC CTCCGCTTCA 
TCCGACACGG AGGAGGACCC CCTTCCTGCC AACAAGTGTA AGAAGGAGAT CATGGTGGGG 
CCTCAGTTCC AAGCCGACCT CAGCGGCCTG CAGTTGGAAC GCCACGGTGA GAAACTCTAC 
GAGAACGAGG ACCAGCTGCT CTGGGACCCC AACGTCCTCC CTGAGAGGGA AGTAGAGGAG 
TTCCTGTACC GGGCGGTGAA GCGGAGGTGG CACGAGATGG CTGGGTCGCA GCTTCCGGAG 
GGAGAGGTGG TGAAAGACAG CGAGCAGGCG CTTTACGAGC TGGTGAAGTG CAACTTCAAC 
GCGGAGGAAG CCCTGCGGAG GCTGCGATTC AACGTGAAGG TGATCCGAGA TGGGTTTTGC 
GCGTGGAGCG AGGAGGAGTG CAGGAACTTT GAGCACGGCT TCCGAGTGCA CGGGAAGAAC 
TTCCATCTGA TCCAGGCCAA CAAGGTGCGC ACGCGGTCTG TGGGCGAGTG CGTGGAGTAC 
TACTACCTGT GGAAGAAGTC TGAGCGCTAC GACTACTTCT CGCAGCAGAC GCGGCTGGGC 
CGGAGGAAAT ACGGCCCGTC TGGAGCCACG GACGCAGACC AGGACCTGGA CGGCAGCGAC 
CCCGATGGCG CAGGCCGCCC CCGCTCCTCG CCGCCTCTGC CCCCTGCTGC CGACGGCCTG 
GCCTCTGAGC AGGACCCGCT GGCGCGGATG CACACGGAGC CGCTGAGCGT AGACAGCGCG 
GCCGGCAGTG TTGGTGAGCC CGGGGAGAGC TCCGACAGCC TCCCGTCCTC GGAGCCGGGG 
CCTTGTCCCT TCCAGCAGCT GGACAGCCCT CCAGCCGCGT CCCTGCCCAG GCGGCCCCCA 
GCCCTGGCCG AGCCCGCCTT CTACCCCCCG GCCACGGCCA CCCCGGAGCC CAGCAGCAGC 
CCGAGACTGG CTGTGGACTT GGCCCTGCCG GGGGCCCTCC CCGAGGAGCT GCCCCTCATC 
TCCAGCCACG CGGACATGAA CGGAGAGCCC GAGGAGGCTG TGGCCCCGGC ACAGGTGGCC 
CTGTCGGTCA CCGAGTTTGG ACTCATCGGC ATTGGGGACG TGAATCCCTT CCTGGCCGCC 
CACCCAGCGT GCCCGGCCCC CGGGCTGCAC TCGGAGCCCC TGTCACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIER2 ( XM_022407334.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022263042.1 Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X3, mRNA.

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CloneID	OCe295779
Clone ID Related Accession (Same CDS sequence)	XM_022407333.1 , XM_022407334.1
Accession Version	XM_022407334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	mesoderm induction early response protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCTCCTCGCT GGAGAGGCAG AGCCCTGGCG TGGCCTCCTG CCTCACTCAC 
AGCCTGTGCC CTGGGGAGCC CAGCTTGCAG ACAGCAGCAG TGGTGTCCAT GGGCTCTGCA 
GACCATCAGT TCAACCTCGT AGAGATCCTG TCCCAGAACT ACGGCATCCG GGAGGAGTGT 
GAGCAGGCCA CGGGAGACAC GGAGAAGCCC GAGGGGGAGC TGGAGAAGGA CTTCATCTCC 
CAGAGCAGCG ACATGCCCCT CGACGAGCTG CTCGCCCTCT ACGGCTATGA GGCCTCAGAC 
CCCATCTCGG AGCAGGGCAG CGAGAGCAGC GACACCACGG CCAACCTCCC GGACATGACG 
CTGGACAAGG AACAAATAGC AAAGGATTTG CTCTCAGGGG AAGAAGAGGA AGAGACACAG 
TCGTCGGCCG ATGACCTTAC CCCGTCCGTG ACCTCCCACG AGGCCTCTGA CCTGTTTCCT 
AACCGGAGTG CATCTTGCTT CCTGGCCGAC GCAGGCAAAG AGCCTGGCTC CTCCGCTTCA 
TCCGACACGG AGGAGGACCC CCTTCCTGCC AACAAGTGTA AGAAGGAGAT CATGGTGGGG 
CCTCAGTTCC AAGCCGACCT CAGCGGCCTG CAGTTGGAAC GCCACGGTGA GAAACTCTAC 
GAGAACGAGG ACCAGCTGCT CTGGGACCCC AACGTCCTCC CTGAGAGGGA AGTAGAGGAG 
TTCCTGTACC GGGCGGTGAA GCGGAGGTGG CACGAGATGG CTGGGTCGCA GCTTCCGGAG 
GGAGAGGTGG TGAAAGACAG CGAGCAGGCG CTTTACGAGC TGGTGAAGTG CAACTTCAAC 
GCGGAGGAAG CCCTGCGGAG GCTGCGATTC AACGTGAAGG TGATCCGAGA TGGGTTTTGC 
GCGTGGAGCG AGGAGGAGTG CAGGAACTTT GAGCACGGCT TCCGAGTGCA CGGGAAGAAC 
TTCCATCTGA TCCAGGCCAA CAAGGTGCGC ACGCGGTCTG TGGGCGAGTG CGTGGAGTAC 
TACTACCTGT GGAAGAAGTC TGAGCGCTAC GACTACTTCT CGCAGCAGAC GCGGCTGGGC 
CGGAGGAAAT ACGGCCCGTC TGGAGCCACG GACGCAGACC AGGACCTGGA CGGCAGCGAC 
CCCGATGGCG CAGGCCGCCC CCGCTCCTCG CCGCCTCTGC CCCCTGCTGC CGACGGCCTG 
GCCTCTGAGC AGGACCCGCT GGCGCGGATG CACACGGAGC CGCTGAGCGT AGACAGCGCG 
GCCGGCAGTG TTGGTGAGCC CGGGGAGAGC TCCGACAGCC TCCCGTCCTC GGAGCCGGGG 
CCTTGTCCCT TCCAGCAGCT GGACAGCCCT CCAGCCGCGT CCCTGCCCAG GCGGCCCCCA 
GCCCTGGCCG AGCCCGCCTT CTACCCCCCG GCCACGGCCA CCCCGGAGCC CAGCAGCAGC 
CCGAGACTGG CTGTGGACTT GGCCCTGCCG GGGGCCCTCC CCGAGGAGCT GCCCCTCATC 
TCCAGCCACG CGGACATGAA CGGAGAGCCC GAGGAGGCTG TGGCCCCGGC ACAGGTGGCC 
CTGTCGGTCA CCGAGTTTGG ACTCATCGGC ATTGGGGACG TGAATCCCTT CCTGGCCGCC 
CACCCAGCGT GCCCGGCCCC CGGGCTGCAC TCGGAGCCCC TGTCACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022263042.1
CDS	44..1714
Translation	MAEASSLERQSPGVASCLTHSLCPGEPSLQTAAVVSMGSADHQFNLVEILSQNYGIREECEQATGDTEKPEGELEKDFISQSSDMPLDELLALYGYEASDPISEQGSESSDTTANLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSASCFLADAGKEPGSSASSDTEEDPLPANKCKKEIMVGPQFQADLSGLQLERHGEKLYENEDQLLWDPNVLPEREVEEFLYRAVKRRWHEMAGSQLPEGEVVKDSEQALYELVKCNFNAEEALRRLRFNVKVIRDGFCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFSQQTRLGRRKYGPSGATDADQDLDGSDPDGAGRPRSSPPLPPAADGLASEQDPLARMHTEPLSVDSAAGSVGEPGESSDSLPSSEPGPCPFQQLDSPPAASLPRRPPALAEPAFYPPATATPEPSSSPRLAVDLALPGALPEELPLISSHADMNGEPEEAVAPAQVALSVTEFGLIGIGDVNPFLAAHPACPAPGLHSEPLSQ

Target ORF information:

RefSeq Version	XM_022407334.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIER family member 2 (MIER2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407334.1

ORF Insert Sequence:

ATGGCGGAGG CCTCCTCGCT GGAGAGGCAG AGCCCTGGCG TGGCCTCCTG CCTCACTCAC 
AGCCTGTGCC CTGGGGAGCC CAGCTTGCAG ACAGCAGCAG TGGTGTCCAT GGGCTCTGCA 
GACCATCAGT TCAACCTCGT AGAGATCCTG TCCCAGAACT ACGGCATCCG GGAGGAGTGT 
GAGCAGGCCA CGGGAGACAC GGAGAAGCCC GAGGGGGAGC TGGAGAAGGA CTTCATCTCC 
CAGAGCAGCG ACATGCCCCT CGACGAGCTG CTCGCCCTCT ACGGCTATGA GGCCTCAGAC 
CCCATCTCGG AGCAGGGCAG CGAGAGCAGC GACACCACGG CCAACCTCCC GGACATGACG 
CTGGACAAGG AACAAATAGC AAAGGATTTG CTCTCAGGGG AAGAAGAGGA AGAGACACAG 
TCGTCGGCCG ATGACCTTAC CCCGTCCGTG ACCTCCCACG AGGCCTCTGA CCTGTTTCCT 
AACCGGAGTG CATCTTGCTT CCTGGCCGAC GCAGGCAAAG AGCCTGGCTC CTCCGCTTCA 
TCCGACACGG AGGAGGACCC CCTTCCTGCC AACAAGTGTA AGAAGGAGAT CATGGTGGGG 
CCTCAGTTCC AAGCCGACCT CAGCGGCCTG CAGTTGGAAC GCCACGGTGA GAAACTCTAC 
GAGAACGAGG ACCAGCTGCT CTGGGACCCC AACGTCCTCC CTGAGAGGGA AGTAGAGGAG 
TTCCTGTACC GGGCGGTGAA GCGGAGGTGG CACGAGATGG CTGGGTCGCA GCTTCCGGAG 
GGAGAGGTGG TGAAAGACAG CGAGCAGGCG CTTTACGAGC TGGTGAAGTG CAACTTCAAC 
GCGGAGGAAG CCCTGCGGAG GCTGCGATTC AACGTGAAGG TGATCCGAGA TGGGTTTTGC 
GCGTGGAGCG AGGAGGAGTG CAGGAACTTT GAGCACGGCT TCCGAGTGCA CGGGAAGAAC 
TTCCATCTGA TCCAGGCCAA CAAGGTGCGC ACGCGGTCTG TGGGCGAGTG CGTGGAGTAC 
TACTACCTGT GGAAGAAGTC TGAGCGCTAC GACTACTTCT CGCAGCAGAC GCGGCTGGGC 
CGGAGGAAAT ACGGCCCGTC TGGAGCCACG GACGCAGACC AGGACCTGGA CGGCAGCGAC 
CCCGATGGCG CAGGCCGCCC CCGCTCCTCG CCGCCTCTGC CCCCTGCTGC CGACGGCCTG 
GCCTCTGAGC AGGACCCGCT GGCGCGGATG CACACGGAGC CGCTGAGCGT AGACAGCGCG 
GCCGGCAGTG TTGGTGAGCC CGGGGAGAGC TCCGACAGCC TCCCGTCCTC GGAGCCGGGG 
CCTTGTCCCT TCCAGCAGCT GGACAGCCCT CCAGCCGCGT CCCTGCCCAG GCGGCCCCCA 
GCCCTGGCCG AGCCCGCCTT CTACCCCCCG GCCACGGCCA CCCCGGAGCC CAGCAGCAGC 
CCGAGACTGG CTGTGGACTT GGCCCTGCCG GGGGCCCTCC CCGAGGAGCT GCCCCTCATC 
TCCAGCCACG CGGACATGAA CGGAGAGCCC GAGGAGGCTG TGGCCCCGGC ACAGGTGGCC 
CTGTCGGTCA CCGAGTTTGG ACTCATCGGC ATTGGGGACG TGAATCCCTT CCTGGCCGCC 
CACCCAGCGT GCCCGGCCCC CGGGCTGCAC TCGGAGCCCC TGTCACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MIER2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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