PLCH2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCH2
Entrez Gene ID	489613
Full Name	phospholipase C eta 2
General protein information	Preferred Names phospholipase C eta 2 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014113868.1	XP_013969343.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
XM_014113866.1	XP_013969341.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_022418930.1	XP_022274638.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_014113866.2	XP_013969341.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
XM_014113867.1	XP_013969342.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
XM_022418931.1	XP_022274639.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0289300	NP_001049792.2
Homo sapiens (human)	PLCH2	NP_055453.2
Pan troglodytes (chimpanzee)	PLCH2	XP_003307816.2
Mus musculus (house mouse)	Plch2	NP_001106831.1
Macaca mulatta (Rhesus monkey)	PLCH2	XP_001085424.2
Arabidopsis thaliana (thale cress)	AT2G40116	NP_850327.2
Xenopus tropicalis (tropical clawed frog)	LOC100487353	XP_002936604.2
Canis lupus familiaris (dog)	PLCH2	XP_005620480.1
Bos taurus (cattle)	PLCH2	XP_005217252.1
Rattus norvegicus (Norway rat)	Plch2	XP_006239682.1
Danio rerio (zebrafish)	plch2	XP_689411.6
Oryza sativa (rice)	Os07g0694000	NP_001060735.1
Gallus gallus (chicken)	PLCH2	XP_425738.3

Gene Ontology

The following PLCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe288361	XM_022418930.1 Latest version!	Canis lupus familiaris phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe288362	XM_022418931.1 Latest version!	Canis lupus familiaris phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe36624	XM_014113866.2 Latest version!	Canis lupus familiaris phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe36625	XM_014113867.1 Latest version!	Canis lupus familiaris phospholipase C, eta 2 (PLCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe36626	XM_014113868.1 Latest version!	Canis lupus familiaris phospholipase C, eta 2 (PLCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OCe36624	XM_014113866.1	Canis lupus familiaris phospholipase C, eta 2 (PLCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCH2 ( XM_022418930.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274638.1 Canis lupus familiaris phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe288361
Clone ID Related Accession (Same CDS sequence)	XM_022418930.1
Accession Version	XM_022418930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCC CGCCAGAGGA CCCAGGGCCC AGTCTGGAGA GTGGACGGCA TTTCCTGAAC 
ATCAATGTTC TGCCTGTGGT GGAGGGATGC ATGAGTGCCA TGCAGGCGGG GACACAGATG 
GTGAAGCTCC GTGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC 
TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAGG CCAAGATTTC CATCGACTCC 
ATCCAGGAGG TGAGCGAGGG TCGGCAGTCG GAGATCTTCC AGCGCTACCC CGATGGCATT 
TTTGACCCCA ACTGCTGCTT CAGCATCTAC CATGGCAGCC ACCGAGAGTC GCTGGACCTG 
GTCTCACCCA GCGGGGACGA GGCGCGCACC TGGGTCACCG GCCTGCGCTA CCTCATGGCA 
GGCATCCGCG ATGAGGACAG CCTGGCCCGC CGCCAGCGCA CTAGGGACCA GTGGCTGAAG 
CAGACATTTG ACGAGGCCGA CAAGAACGGG GACGGCAGCC TGAGCATCGG TGAGGTCCTG 
CAGCTGCTGC ATAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG 
GAAGCAGATA CAGATGACCA CCAGGGCACA CTGGGCTTTG AGGAGTTCTG TGCCTTCTAT 
AAGATGATGT CCACCCGCCG GGACCTCTAC CTGCTCATGC TGACCTACAG TGACCACAAG 
GACCACCTGA ACGCCACTGA CCTCCAGCGC TTCCTGGAGG TGGAGCAGAA GATGGCGGGT 
GTGACCCTTG AGAGCTGCCG GGACATCATC GAGCAGTTTG AGCCCTGCCC AGAAAACAAG 
AGTAAGGGGG TGCTGGGCAT TGATGGCTTC ACCAACTATA CTCGGAGTCC CGCCGGCGAC 
ATCTTTAACC CAGAGCACCA TGGCGTGCAC CAGGACATGA CACGGCCGCT AAGCCACTAC 
TTCATCACCT CATCCCACAA CACCTACCTC GTGGGTGACC AGCTCATGTC CCAGTCTCGG 
GTAGACATGT ACGCCTGGGT CCTGCAGGCC GGCTGCCGTT GCGTGGAGGT GGACTGCTGG 
GATGGGCCTG ACGGGGAGCC CATTGTGCAC CATGGCTATA CTCTGACCTC CAAGATCCTC 
TTCAGAGATG TCATTGAAAC CATCAACAAA TATGCTTTTG TCAAGAATGA GTACCCAGTG 
ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAACAGA AGAAGATGGC CCAGTATCTA 
ACTGATATTC TTGGAGATAA GCTGGACGTG TCATCAGTAA GCAGTGACGA TGCCACCTTG 
CTTCCTTCTC CACAGATGCT CAAGGGAAAG ATTCTGGTGA AGGGCAAGAA GCTTCCTGCC 
AACATCAGTG AGGATGCTGA AGAGGGCGAG GTGTCTGACG AAGACAGTGC AGATGAGATT 
GACGAGGACT GCAAACTTCT CAATGGGGAT GTTGCCACCA ACCGGAAGCG TGTAGAAAAC 
ATTGCCAAGA AGAAACTGGA TTCCCTGATG AAGGAGTCAA AGATTCGGGA CTGCGAGGAC 
CCAAATGACT TCACTGTGTC CACGCTGCCC CCGTCTGGAA AGCTTGGGTA CAAGGCAGAA 
GGCAAAAAGG CTGAGGAGGA TGTGGAGTCC GGGGAGGACG CCGGGGTCAG CAGACGGAAC 
AACCGGATCC TCATGAGCGG TTTCTCCAAG CGCAAGAAGA AGAGCAGCAA GCTAAAGAAG 
GCGGCCAGCA TGGAGGAGGG GGATGAGGAC CTTGACTCCC AGGGCAGTCA GAGTCGAGGG 
GCAAGCCGAC AGAAGAAGAC CATGAAGCTG TCCCGTGCCC TCTCGGACCT GGTGAAGTAC 
ACCAAGTCTG TGGGCACCCA CGACGTGGAG GCAGAGGTGG CGTCCAGCTG GCAGGTGTCG 
TCCTTCAGTG AGACCAGGGC CCAGCAGATC CTGCAGCAGA AGCCGGCACA GTACCTGCGC 
TTCAACCAGC ACCAGCTCTC CCGCATCTAC CCCTCCTCCT ACCGCGTGGA CTCCAGCAAC 
TACAACCCGC AGCCCTTCTG GAACGCAGGC TGCCAGATGG TCGCCCTGAA CTACCAGTCA 
GAGGGGCGGA TGCTGCAGCT GAACCGCGCC AAGTTCAGTG CCAATGGGAA CTGTGGCTAT 
GTGCTCAAGC CCCAGTGCAT GTGCCAGGGT GTCTTCAACC CCAACTCCGA GGACCCCCTG 
CCCGGGCAGC TCAAGAAGCA GCTGGTGCTT CGGATCATCA GTGGGCAGCA GCTCCCCAAG 
CCAAGGGACT CGATGCTGGG AGACCGAGGC GAGATCATCG ACCCCTTCGT GGAGGTGGAG 
GTCATCGGGC TTCCCGTGGA TTGCAACAGG GAGCAGACGC GTGTGGTGGA TGACAATGGA 
TTCAACCCCA TGTGGGAAGA GACCCTGGTG TTCACTGTGC ACATGCCTGA GATCGCACTG 
GTGCGCTTCC TCGTCTGGGA CCATGACCCC ATTGGGCGTG ACTTCATTGG CCAGAGGACA 
CTGGCCTTCA GCAGCATGAT GCCAGGCTAT CGGCACGTGT ACCTAGAGGG GATGGAAGAG 
GCCTCCATCT TTGTCCATGT GGCCGTCAGT GACATCAGTG GTAAGGTCAA GCAGGCTCTG 
GGCCTAAAAG GCCTGTTCCT CAGAGGCACA AAGCCCGGCT CCCTGGACAG TCATGCTGCT 
GGGCGGCCCC TGCCCCGGCC TTCCGTTAGC CAGCGGCTCC TGCGGCGCAC GGCCAGCGCC 
CCGACCAAGA GCCAGAAACC CAGCCGCAAG GCCTTCCCAG AGCTGGTCCT GGGCACGCAG 
GACACGGGCT CCGAGGGGGA GGCCCGTGAC GTGGCCGCCC CCAGCCCTGG CCCCGTCCTA 
GAGGCCTCAG CCCCAGAGGA GCCCGGCAGC CGCAGCCCCC GAGATACCCG CCCCTTCTCC 
ATGCAGAGGA CGGGCTCCTC TCTATGTGGC CTGGAAACTA TTGCTGAGGA GCCAGCCCTG 
GGCCCCGGCC CCCCACCTCT GGTGGCTGCC CCCCCCAGCC CCTCCCCGGG AGGGCCCCTG 
TGCGCCTCGG GACCTGGCAC CAAAGTGACG AGCCCCCCAG CGGTCACTCT GGGAGCTTCC 
GTGCCCTTCC AGCCCAGGAC CCGGAGCAGA GGGGACACTG AGCAGCCCCC GGAAAGCCGG 
CGGCAGGGTT ACCATGGTGG GGGGCCTGGA GGTGTGTGTG AGGGGACCCC TAGCAGCCAG 
GTGGTCAGAA GGGCCGAGAG TGAGGGGCAG GTGCCCGCGG AGCACCCGGG TGGATGGCGG 
CCCCCAGCCG GGCCCTGCCC AGCCGTGTAC TCAGACGCCA CAGGTGGAGA CCGGCTGTGG 
CGGCGGCTGG AACCTGGTGG CCATCGCGAC AGCGTGTCCT CGTCCTCCAG TGTGTCATCC 
AGCGACACGG TCATCGACCT CTCCCTGCCG GGCCTGGGTC TGGGCCTCGT CCTGGGCCGT 
GAGGGTGTCT CGGGGGGTGT CTCGGGGGCT CCAGCTGGCC GCCTGCCCCT GCGGCCCTGC 
CCAGCCACTG CCGCCCGCCT GGACCTGCCT GCCGTGACCA AGAGCAAATC CAGCCCCAAC 
CTGCGGGCCG CCAGCCAGCT GCCCGCTGCA CCAGAAGAGC TTCGGCTGCG TCCCCTGGTG 
CCCAGGCTGC CCTGGGGCCA CCTTCCCCTG GCAGGCCTCC GGGACTGCCC TGCAGCCGCC 
AAGTCTAAGA GCCTGGGGGA CCTGACTGCC GACGACTTTG CCCCCCAGGT GGAGATCCTG 
GGCCGAAGCC TGGGCCTGGC GAGGGAGGGG CGGGCAGGGC GGGGGGCGCG GCGGGACGCC 
CTGACGGAAC AGCTGCGCTG GCTCACAGGC TTCCAGCAGG CCGGTGACAT CACCTCGCCC 
ACCAGCCTGA GTGTCGCTGG GGATGGGGTG CCTGGTCCCC CTGGCTTCCT GCGGCGCTCC 
TCCTCCCGCA GCCAGAGCCG CGTGCGTGCC ATTGCCAGCC GGGCCAGGCA GGCCCAGGAG 
CGGCAGCAAC GGCTGCAGAG CCCGAGGGCG CCCCCCGGGG AGGAGCGGGG CACCCCCGAG 
GGTGCCTGCT CAGGGGGCCA AGGGGGCTGC GGGGACGTGC CGGGCATGGG CCCCTCCAAG 
GGCTCCGCTG CCCCCGGCCT CCTGCTCAGA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274638.1
CDS	314..4489
Translation	MAPPPEDPGPSLESGRHFLNINVLPVVEGCMSAMQAGTQMVKLRGSSKGLVRFYFLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDGIFDPNCCFSIYHGSHRESLDLVSPSGDEARTWVTGLRYLMAGIRDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFKEADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSDHKDHLNATDLQRFLEVEQKMAGVTLESCRDIIEQFEPCPENKSKGVLGIDGFTNYTRSPAGDIFNPEHHGVHQDMTRPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFRDVIETINKYAFVKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDVSSVSSDDATLLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDEDCKLLNGDVATNRKRVENIAKKKLDSLMKESKIRDCEDPNDFTVSTLPPSGKLGYKAEGKKAEEDVESGEDAGVSRRNNRILMSGFSKRKKKSSKLKKAASMEEGDEDLDSQGSQSRGASRQKKTMKLSRALSDLVKYTKSVGTHDVEAEVASSWQVSSFSETRAQQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGNCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCNREQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGTKPGSLDSHAAGRPLPRPSVSQRLLRRTASAPTKSQKPSRKAFPELVLGTQDTGSEGEARDVAAPSPGPVLEASAPEEPGSRSPRDTRPFSMQRTGSSLCGLETIAEEPALGPGPPPLVAAPPSPSPGGPLCASGPGTKVTSPPAVTLGASVPFQPRTRSRGDTEQPPESRRQGYHGGGPGGVCEGTPSSQVVRRAESEGQVPAEHPGGWRPPAGPCPAVYSDATGGDRLWRRLEPGGHRDSVSSSSSVSSSDTVIDLSLPGLGLGLVLGREGVSGGVSGAPAGRLPLRPCPATAARLDLPAVTKSKSSPNLRAASQLPAAPEELRLRPLVPRLPWGHLPLAGLRDCPAAAKSKSLGDLTADDFAPQVEILGRSLGLAREGRAGRGARRDALTEQLRWLTGFQQAGDITSPTSLSVAGDGVPGPPGFLRRSSSRSQSRVRAIASRARQAQERQQRLQSPRAPPGEERGTPEGACSGGQGGCGDVPGMGPSKGSAAPGLLLRL

Target ORF information:

RefSeq Version	XM_022418930.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418930.1

ORF Insert Sequence:

ATGGCTCCCC CGCCAGAGGA CCCAGGGCCC AGTCTGGAGA GTGGACGGCA TTTCCTGAAC 
ATCAATGTTC TGCCTGTGGT GGAGGGATGC ATGAGTGCCA TGCAGGCGGG GACACAGATG 
GTGAAGCTCC GTGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC 
TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAGG CCAAGATTTC CATCGACTCC 
ATCCAGGAGG TGAGCGAGGG TCGGCAGTCG GAGATCTTCC AGCGCTACCC CGATGGCATT 
TTTGACCCCA ACTGCTGCTT CAGCATCTAC CATGGCAGCC ACCGAGAGTC GCTGGACCTG 
GTCTCACCCA GCGGGGACGA GGCGCGCACC TGGGTCACCG GCCTGCGCTA CCTCATGGCA 
GGCATCCGCG ATGAGGACAG CCTGGCCCGC CGCCAGCGCA CTAGGGACCA GTGGCTGAAG 
CAGACATTTG ACGAGGCCGA CAAGAACGGG GACGGCAGCC TGAGCATCGG TGAGGTCCTG 
CAGCTGCTGC ATAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG 
GAAGCAGATA CAGATGACCA CCAGGGCACA CTGGGCTTTG AGGAGTTCTG TGCCTTCTAT 
AAGATGATGT CCACCCGCCG GGACCTCTAC CTGCTCATGC TGACCTACAG TGACCACAAG 
GACCACCTGA ACGCCACTGA CCTCCAGCGC TTCCTGGAGG TGGAGCAGAA GATGGCGGGT 
GTGACCCTTG AGAGCTGCCG GGACATCATC GAGCAGTTTG AGCCCTGCCC AGAAAACAAG 
AGTAAGGGGG TGCTGGGCAT TGATGGCTTC ACCAACTATA CTCGGAGTCC CGCCGGCGAC 
ATCTTTAACC CAGAGCACCA TGGCGTGCAC CAGGACATGA CACGGCCGCT AAGCCACTAC 
TTCATCACCT CATCCCACAA CACCTACCTC GTGGGTGACC AGCTCATGTC CCAGTCTCGG 
GTAGACATGT ACGCCTGGGT CCTGCAGGCC GGCTGCCGTT GCGTGGAGGT GGACTGCTGG 
GATGGGCCTG ACGGGGAGCC CATTGTGCAC CATGGCTATA CTCTGACCTC CAAGATCCTC 
TTCAGAGATG TCATTGAAAC CATCAACAAA TATGCTTTTG TCAAGAATGA GTACCCAGTG 
ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAACAGA AGAAGATGGC CCAGTATCTA 
ACTGATATTC TTGGAGATAA GCTGGACGTG TCATCAGTAA GCAGTGACGA TGCCACCTTG 
CTTCCTTCTC CACAGATGCT CAAGGGAAAG ATTCTGGTGA AGGGCAAGAA GCTTCCTGCC 
AACATCAGTG AGGATGCTGA AGAGGGCGAG GTGTCTGACG AAGACAGTGC AGATGAGATT 
GACGAGGACT GCAAACTTCT CAATGGGGAT GTTGCCACCA ACCGGAAGCG TGTAGAAAAC 
ATTGCCAAGA AGAAACTGGA TTCCCTGATG AAGGAGTCAA AGATTCGGGA CTGCGAGGAC 
CCAAATGACT TCACTGTGTC CACGCTGCCC CCGTCTGGAA AGCTTGGGTA CAAGGCAGAA 
GGCAAAAAGG CTGAGGAGGA TGTGGAGTCC GGGGAGGACG CCGGGGTCAG CAGACGGAAC 
AACCGGATCC TCATGAGCGG TTTCTCCAAG CGCAAGAAGA AGAGCAGCAA GCTAAAGAAG 
GCGGCCAGCA TGGAGGAGGG GGATGAGGAC CTTGACTCCC AGGGCAGTCA GAGTCGAGGG 
GCAAGCCGAC AGAAGAAGAC CATGAAGCTG TCCCGTGCCC TCTCGGACCT GGTGAAGTAC 
ACCAAGTCTG TGGGCACCCA CGACGTGGAG GCAGAGGTGG CGTCCAGCTG GCAGGTGTCG 
TCCTTCAGTG AGACCAGGGC CCAGCAGATC CTGCAGCAGA AGCCGGCACA GTACCTGCGC 
TTCAACCAGC ACCAGCTCTC CCGCATCTAC CCCTCCTCCT ACCGCGTGGA CTCCAGCAAC 
TACAACCCGC AGCCCTTCTG GAACGCAGGC TGCCAGATGG TCGCCCTGAA CTACCAGTCA 
GAGGGGCGGA TGCTGCAGCT GAACCGCGCC AAGTTCAGTG CCAATGGGAA CTGTGGCTAT 
GTGCTCAAGC CCCAGTGCAT GTGCCAGGGT GTCTTCAACC CCAACTCCGA GGACCCCCTG 
CCCGGGCAGC TCAAGAAGCA GCTGGTGCTT CGGATCATCA GTGGGCAGCA GCTCCCCAAG 
CCAAGGGACT CGATGCTGGG AGACCGAGGC GAGATCATCG ACCCCTTCGT GGAGGTGGAG 
GTCATCGGGC TTCCCGTGGA TTGCAACAGG GAGCAGACGC GTGTGGTGGA TGACAATGGA 
TTCAACCCCA TGTGGGAAGA GACCCTGGTG TTCACTGTGC ACATGCCTGA GATCGCACTG 
GTGCGCTTCC TCGTCTGGGA CCATGACCCC ATTGGGCGTG ACTTCATTGG CCAGAGGACA 
CTGGCCTTCA GCAGCATGAT GCCAGGCTAT CGGCACGTGT ACCTAGAGGG GATGGAAGAG 
GCCTCCATCT TTGTCCATGT GGCCGTCAGT GACATCAGTG GTAAGGTCAA GCAGGCTCTG 
GGCCTAAAAG GCCTGTTCCT CAGAGGCACA AAGCCCGGCT CCCTGGACAG TCATGCTGCT 
GGGCGGCCCC TGCCCCGGCC TTCCGTTAGC CAGCGGCTCC TGCGGCGCAC GGCCAGCGCC 
CCGACCAAGA GCCAGAAACC CAGCCGCAAG GCCTTCCCAG AGCTGGTCCT GGGCACGCAG 
GACACGGGCT CCGAGGGGGA GGCCCGTGAC GTGGCCGCCC CCAGCCCTGG CCCCGTCCTA 
GAGGCCTCAG CCCCAGAGGA GCCCGGCAGC CGCAGCCCCC GAGATACCCG CCCCTTCTCC 
ATGCAGAGGA CGGGCTCCTC TCTATGTGGC CTGGAAACTA TTGCTGAGGA GCCAGCCCTG 
GGCCCCGGCC CCCCACCTCT GGTGGCTGCC CCCCCCAGCC CCTCCCCGGG AGGGCCCCTG 
TGCGCCTCGG GACCTGGCAC CAAAGTGACG AGCCCCCCAG CGGTCACTCT GGGAGCTTCC 
GTGCCCTTCC AGCCCAGGAC CCGGAGCAGA GGGGACACTG AGCAGCCCCC GGAAAGCCGG 
CGGCAGGGTT ACCATGGTGG GGGGCCTGGA GGTGTGTGTG AGGGGACCCC TAGCAGCCAG 
GTGGTCAGAA GGGCCGAGAG TGAGGGGCAG GTGCCCGCGG AGCACCCGGG TGGATGGCGG 
CCCCCAGCCG GGCCCTGCCC AGCCGTGTAC TCAGACGCCA CAGGTGGAGA CCGGCTGTGG 
CGGCGGCTGG AACCTGGTGG CCATCGCGAC AGCGTGTCCT CGTCCTCCAG TGTGTCATCC 
AGCGACACGG TCATCGACCT CTCCCTGCCG GGCCTGGGTC TGGGCCTCGT CCTGGGCCGT 
GAGGGTGTCT CGGGGGGTGT CTCGGGGGCT CCAGCTGGCC GCCTGCCCCT GCGGCCCTGC 
CCAGCCACTG CCGCCCGCCT GGACCTGCCT GCCGTGACCA AGAGCAAATC CAGCCCCAAC 
CTGCGGGCCG CCAGCCAGCT GCCCGCTGCA CCAGAAGAGC TTCGGCTGCG TCCCCTGGTG 
CCCAGGCTGC CCTGGGGCCA CCTTCCCCTG GCAGGCCTCC GGGACTGCCC TGCAGCCGCC 
AAGTCTAAGA GCCTGGGGGA CCTGACTGCC GACGACTTTG CCCCCCAGGT GGAGATCCTG 
GGCCGAAGCC TGGGCCTGGC GAGGGAGGGG CGGGCAGGGC GGGGGGCGCG GCGGGACGCC 
CTGACGGAAC AGCTGCGCTG GCTCACAGGC TTCCAGCAGG CCGGTGACAT CACCTCGCCC 
ACCAGCCTGA GTGTCGCTGG GGATGGGGTG CCTGGTCCCC CTGGCTTCCT GCGGCGCTCC 
TCCTCCCGCA GCCAGAGCCG CGTGCGTGCC ATTGCCAGCC GGGCCAGGCA GGCCCAGGAG 
CGGCAGCAAC GGCTGCAGAG CCCGAGGGCG CCCCCCGGGG AGGAGCGGGG CACCCCCGAG 
GGTGCCTGCT CAGGGGGCCA AGGGGGCTGC GGGGACGTGC CGGGCATGGG CCCCTCCAAG 
GGCTCCGCTG CCCCCGGCCT CCTGCTCAGA CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_022418931.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274639.1 Canis lupus familiaris phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe288362
Clone ID Related Accession (Same CDS sequence)	XM_022418931.1
Accession Version	XM_022418931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCC CGCCAGAGGA CCCAGGGCCC AGTCTGGAGA GTGGACGGCA TTTCCTGAAC 
ATCAATGTTC TGCCTGTGGT GGAGGGATGC ATGAGTGCCA TGCAGGCGGG GACACAGATG 
GTGAAGCTCC GTGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC 
TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAGG CCAAGATTTC CATCGACTCC 
ATCCAGGAGG TGAGCGAGGG TCGGCAGTCG GAGATCTTCC AGCGCTACCC CGATGGCATT 
TTTGACCCCA ACTGCTGCTT CAGCATCTAC CATGGCAGCC ACCGAGAGTC GCTGGACCTG 
GTCTCACCCA GCGGGGACGA GGCGCGCACC TGGGTCACCG GCCTGCGCTA CCTCATGGCA 
GGCATCCGCG ATGAGGACAG CCTGGCCCGC CGCCAGCGCA CTAGGGACCA GTGGCTGAAG 
CAGACATTTG ACGAGGCCGA CAAGAACGGG GACGGCAGCC TGAGCATCGG TGAGGTCCTG 
CAGCTGCTGC ATAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG 
GAAGCAGATA CAGATGACCA CCAGGGCACA CTGGGCTTTG AGGAGTTCTG TGCCTTCTAT 
AAGATGATGT CCACCCGCCG GGACCTCTAC CTGCTCATGC TGACCTACAG TGACCACAAG 
GACCACCTGA ACGCCACTGA CCTCCAGCGC TTCCTGGAGG TGGAGCAGAA GATGGCGGGT 
GTGACCCTTG AGAGCTGCCG GGACATCATC GAGCAGTTTG AGCCCTGCCC AGAAAACAAG 
AGTAAGGGGG TGCTGGGCAT TGATGGCTTC ACCAACTATA CTCGGAGTCC CGCCGGCGAC 
ATCTTTAACC CAGAGCACCA TGGCGTGCAC CAGGACATGA CACGGCCGCT AAGCCACTAC 
TTCATCACCT CATCCCACAA CACCTACCTC GTGGGTGACC AGCTCATGTC CCAGTCTCGG 
GTAGACATGT ACGCCTGGGT CCTGCAGGCC GGCTGCCGTT GCGTGGAGGT GGACTGCTGG 
GATGGGCCTG ACGGGGAGCC CATTGTGCAC CATGGCTATA CTCTGACCTC CAAGATCCTC 
TTCAGAGATG TCATTGAAAC CATCAACAAA TATGCTTTTG TCAAGAATGA GTACCCAGTG 
ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAACAGA AGAAGATGGC CCAGTATCTA 
ACTGATATTC TTGGAGATAA GCTGGACGTG TCATCAGTAA GCAGTGACGA TGCCACCTTG 
CTTCCTTCTC CACAGATGCT CAAGGGAAAG ATTCTGGTGA AGGGCAAGAA GCTTCCTGCC 
AACATCAGTG AGGATGCTGA AGAGGGCGAG GTGTCTGACG AAGACAGTGC AGATGAGATT 
GACGAGGACT GCAAACTTCT CAATGGGGAT GTTGCCACCA ACCGGAAGCG TGTAGAAAAC 
ATTGCCAAGA AGAAACTGGA TTCCCTGATG AAGGAGTCAA AGATTCGGGA CTGCGAGGAC 
CCAAATGACT TCACTGTGTC CACGCTGCCC CCGTCTGGAA AGCTTGGGTA CAAGGCAGAA 
GGCAAAAAGG CTGAGGAGGA TGTGGAGTCC GGGGAGGACG CCGGGGTCAG CAGACGGAAC 
AACCGGATCC TCATGAGCGG TTTCTCCAAG CGCAAGAAGA GCAGCAAGCT AAAGAAGGCG 
GCCAGCATGG AGGAGGGGGA TGAGGACCTT GACTCCCAGG GCAGTCAGAG TCGAGGGGCA 
AGCCGACAGA AGAAGACCAT GAAGCTGTCC CGTGCCCTCT CGGACCTGGT GAAGTACACC 
AAGTCTGTGG GCACCCACGA CGTGGAGGCA GAGGTGGCGT CCAGCTGGCA GGTGTCGTCC 
TTCAGTGAGA CCAGGGCCCA GCAGATCCTG CAGCAGAAGC CGGCACAGTA CCTGCGCTTC 
AACCAGCACC AGCTCTCCCG CATCTACCCC TCCTCCTACC GCGTGGACTC CAGCAACTAC 
AACCCGCAGC CCTTCTGGAA CGCAGGCTGC CAGATGGTCG CCCTGAACTA CCAGTCAGAG 
GGGCGGATGC TGCAGCTGAA CCGCGCCAAG TTCAGTGCCA ATGGGAACTG TGGCTATGTG 
CTCAAGCCCC AGTGCATGTG CCAGGGTGTC TTCAACCCCA ACTCCGAGGA CCCCCTGCCC 
GGGCAGCTCA AGAAGCAGCT GGTGCTTCGG ATCATCAGTG GGCAGCAGCT CCCCAAGCCA 
AGGGACTCGA TGCTGGGAGA CCGAGGCGAG ATCATCGACC CCTTCGTGGA GGTGGAGGTC 
ATCGGGCTTC CCGTGGATTG CAACAGGGAG CAGACGCGTG TGGTGGATGA CAATGGATTC 
AACCCCATGT GGGAAGAGAC CCTGGTGTTC ACTGTGCACA TGCCTGAGAT CGCACTGGTG 
CGCTTCCTCG TCTGGGACCA TGACCCCATT GGGCGTGACT TCATTGGCCA GAGGACACTG 
GCCTTCAGCA GCATGATGCC AGGCTATCGG CACGTGTACC TAGAGGGGAT GGAAGAGGCC 
TCCATCTTTG TCCATGTGGC CGTCAGTGAC ATCAGTGGTA AGGTCAAGCA GGCTCTGGGC 
CTAAAAGGCC TGTTCCTCAG AGGCACAAAG CCCGGCTCCC TGGACAGTCA TGCTGCTGGG 
CGGCCCCTGC CCCGGCCTTC CGTTAGCCAG CGGCTCCTGC GGCGCACGGC CAGCGCCCCG 
ACCAAGAGCC AGAAACCCAG CCGCAAGGCC TTCCCAGAGC TGGTCCTGGG CACGCAGGAC 
ACGGGCTCCG AGGGGGAGGC CCGTGACGTG GCCGCCCCCA GCCCTGGCCC CGTCCTAGAG 
GCCTCAGCCC CAGAGGAGCC CGGCAGCCGC AGCCCCCGAG ATACCCGCCC CTTCTCCATG 
CAGAGGACGG GCTCCTCTCT ATGTGGCCTG GAAACTATTG CTGAGGAGCC AGCCCTGGGC 
CCCGGCCCCC CACCTCTGGT GGCTGCCCCC CCCAGCCCCT CCCCGGGAGG GCCCCTGTGC 
GCCTCGGGAC CTGGCACCAA AGTGACGAGC CCCCCAGCGG TCACTCTGGG AGCTTCCGTG 
CCCTTCCAGC CCAGGACCCG GAGCAGAGGG GACACTGAGC AGCCCCCGGA AAGCCGGCGG 
CAGGGTTACC ATGGTGGGGG GCCTGGAGGT GTGTGTGAGG GGACCCCTAG CAGCCAGGTG 
GTCAGAAGGG CCGAGAGTGA GGGGCAGGTG CCCGCGGAGC ACCCGGGTGG ATGGCGGCCC 
CCAGCCGGGC CCTGCCCAGC CGTGTACTCA GACGCCACAG GTGGAGACCG GCTGTGGCGG 
CGGCTGGAAC CTGGTGGCCA TCGCGACAGC GTGTCCTCGT CCTCCAGTGT GTCATCCAGC 
GACACGGTCA TCGACCTCTC CCTGCCGGGC CTGGGTCTGG GCCTCGTCCT GGGCCGTGAG 
GGTGTCTCGG GGGGTGTCTC GGGGGCTCCA GCTGGCCGCC TGCCCCTGCG GCCCTGCCCA 
GCCACTGCCG CCCGCCTGGA CCTGCCTGCC GTGACCAAGA GCAAATCCAG CCCCAACCTG 
CGGGCCGCCA GCCAGCTGCC CGCTGCACCA GAAGAGCTTC GGCTGCGTCC CCTGGTGCCC 
AGGCTGCCCT GGGGCCACCT TCCCCTGGCA GGCCTCCGGG ACTGCCCTGC AGCCGCCAAG 
TCTAAGAGCC TGGGGGACCT GACTGCCGAC GACTTTGCCC CCCAGGTGGA GATCCTGGGC 
CGAAGCCTGG GCCTGGCGAG GGAGGGGCGG GCAGGGCGGG GGGCGCGGCG GGACGCCCTG 
ACGGAACAGC TGCGCTGGCT CACAGGCTTC CAGCAGGCCG GTGACATCAC CTCGCCCACC 
AGCCTGAGTG TCGCTGGGGA TGGGGTGCCT GGTCCCCCTG GCTTCCTGCG GCGCTCCTCC 
TCCCGCAGCC AGAGCCGCGT GCGTGCCATT GCCAGCCGGG CCAGGCAGGC CCAGGAGCGG 
CAGCAACGGC TGCAGAGCCC GAGGGCGCCC CCCGGGGAGG AGCGGGGCAC CCCCGAGGGT 
GCCTGCTCAG GGGGCCAAGG GGGCTGCGGG GACGTGCCGG GCATGGGCCC CTCCAAGGGC 
TCCGCTGCCC CCGGCCTCCT GCTCAGACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274639.1
CDS	315..4487
Translation	MAPPPEDPGPSLESGRHFLNINVLPVVEGCMSAMQAGTQMVKLRGSSKGLVRFYFLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDGIFDPNCCFSIYHGSHRESLDLVSPSGDEARTWVTGLRYLMAGIRDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFKEADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSDHKDHLNATDLQRFLEVEQKMAGVTLESCRDIIEQFEPCPENKSKGVLGIDGFTNYTRSPAGDIFNPEHHGVHQDMTRPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFRDVIETINKYAFVKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDVSSVSSDDATLLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDEDCKLLNGDVATNRKRVENIAKKKLDSLMKESKIRDCEDPNDFTVSTLPPSGKLGYKAEGKKAEEDVESGEDAGVSRRNNRILMSGFSKRKKSSKLKKAASMEEGDEDLDSQGSQSRGASRQKKTMKLSRALSDLVKYTKSVGTHDVEAEVASSWQVSSFSETRAQQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGNCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCNREQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGTKPGSLDSHAAGRPLPRPSVSQRLLRRTASAPTKSQKPSRKAFPELVLGTQDTGSEGEARDVAAPSPGPVLEASAPEEPGSRSPRDTRPFSMQRTGSSLCGLETIAEEPALGPGPPPLVAAPPSPSPGGPLCASGPGTKVTSPPAVTLGASVPFQPRTRSRGDTEQPPESRRQGYHGGGPGGVCEGTPSSQVVRRAESEGQVPAEHPGGWRPPAGPCPAVYSDATGGDRLWRRLEPGGHRDSVSSSSSVSSSDTVIDLSLPGLGLGLVLGREGVSGGVSGAPAGRLPLRPCPATAARLDLPAVTKSKSSPNLRAASQLPAAPEELRLRPLVPRLPWGHLPLAGLRDCPAAAKSKSLGDLTADDFAPQVEILGRSLGLAREGRAGRGARRDALTEQLRWLTGFQQAGDITSPTSLSVAGDGVPGPPGFLRRSSSRSQSRVRAIASRARQAQERQQRLQSPRAPPGEERGTPEGACSGGQGGCGDVPGMGPSKGSAAPGLLLRL

Target ORF information:

RefSeq Version	XM_022418931.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418931.1

ORF Insert Sequence:

ATGGCTCCCC CGCCAGAGGA CCCAGGGCCC AGTCTGGAGA GTGGACGGCA TTTCCTGAAC 
ATCAATGTTC TGCCTGTGGT GGAGGGATGC ATGAGTGCCA TGCAGGCGGG GACACAGATG 
GTGAAGCTCC GTGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC 
TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAGG CCAAGATTTC CATCGACTCC 
ATCCAGGAGG TGAGCGAGGG TCGGCAGTCG GAGATCTTCC AGCGCTACCC CGATGGCATT 
TTTGACCCCA ACTGCTGCTT CAGCATCTAC CATGGCAGCC ACCGAGAGTC GCTGGACCTG 
GTCTCACCCA GCGGGGACGA GGCGCGCACC TGGGTCACCG GCCTGCGCTA CCTCATGGCA 
GGCATCCGCG ATGAGGACAG CCTGGCCCGC CGCCAGCGCA CTAGGGACCA GTGGCTGAAG 
CAGACATTTG ACGAGGCCGA CAAGAACGGG GACGGCAGCC TGAGCATCGG TGAGGTCCTG 
CAGCTGCTGC ATAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG 
GAAGCAGATA CAGATGACCA CCAGGGCACA CTGGGCTTTG AGGAGTTCTG TGCCTTCTAT 
AAGATGATGT CCACCCGCCG GGACCTCTAC CTGCTCATGC TGACCTACAG TGACCACAAG 
GACCACCTGA ACGCCACTGA CCTCCAGCGC TTCCTGGAGG TGGAGCAGAA GATGGCGGGT 
GTGACCCTTG AGAGCTGCCG GGACATCATC GAGCAGTTTG AGCCCTGCCC AGAAAACAAG 
AGTAAGGGGG TGCTGGGCAT TGATGGCTTC ACCAACTATA CTCGGAGTCC CGCCGGCGAC 
ATCTTTAACC CAGAGCACCA TGGCGTGCAC CAGGACATGA CACGGCCGCT AAGCCACTAC 
TTCATCACCT CATCCCACAA CACCTACCTC GTGGGTGACC AGCTCATGTC CCAGTCTCGG 
GTAGACATGT ACGCCTGGGT CCTGCAGGCC GGCTGCCGTT GCGTGGAGGT GGACTGCTGG 
GATGGGCCTG ACGGGGAGCC CATTGTGCAC CATGGCTATA CTCTGACCTC CAAGATCCTC 
TTCAGAGATG TCATTGAAAC CATCAACAAA TATGCTTTTG TCAAGAATGA GTACCCAGTG 
ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAACAGA AGAAGATGGC CCAGTATCTA 
ACTGATATTC TTGGAGATAA GCTGGACGTG TCATCAGTAA GCAGTGACGA TGCCACCTTG 
CTTCCTTCTC CACAGATGCT CAAGGGAAAG ATTCTGGTGA AGGGCAAGAA GCTTCCTGCC 
AACATCAGTG AGGATGCTGA AGAGGGCGAG GTGTCTGACG AAGACAGTGC AGATGAGATT 
GACGAGGACT GCAAACTTCT CAATGGGGAT GTTGCCACCA ACCGGAAGCG TGTAGAAAAC 
ATTGCCAAGA AGAAACTGGA TTCCCTGATG AAGGAGTCAA AGATTCGGGA CTGCGAGGAC 
CCAAATGACT TCACTGTGTC CACGCTGCCC CCGTCTGGAA AGCTTGGGTA CAAGGCAGAA 
GGCAAAAAGG CTGAGGAGGA TGTGGAGTCC GGGGAGGACG CCGGGGTCAG CAGACGGAAC 
AACCGGATCC TCATGAGCGG TTTCTCCAAG CGCAAGAAGA GCAGCAAGCT AAAGAAGGCG 
GCCAGCATGG AGGAGGGGGA TGAGGACCTT GACTCCCAGG GCAGTCAGAG TCGAGGGGCA 
AGCCGACAGA AGAAGACCAT GAAGCTGTCC CGTGCCCTCT CGGACCTGGT GAAGTACACC 
AAGTCTGTGG GCACCCACGA CGTGGAGGCA GAGGTGGCGT CCAGCTGGCA GGTGTCGTCC 
TTCAGTGAGA CCAGGGCCCA GCAGATCCTG CAGCAGAAGC CGGCACAGTA CCTGCGCTTC 
AACCAGCACC AGCTCTCCCG CATCTACCCC TCCTCCTACC GCGTGGACTC CAGCAACTAC 
AACCCGCAGC CCTTCTGGAA CGCAGGCTGC CAGATGGTCG CCCTGAACTA CCAGTCAGAG 
GGGCGGATGC TGCAGCTGAA CCGCGCCAAG TTCAGTGCCA ATGGGAACTG TGGCTATGTG 
CTCAAGCCCC AGTGCATGTG CCAGGGTGTC TTCAACCCCA ACTCCGAGGA CCCCCTGCCC 
GGGCAGCTCA AGAAGCAGCT GGTGCTTCGG ATCATCAGTG GGCAGCAGCT CCCCAAGCCA 
AGGGACTCGA TGCTGGGAGA CCGAGGCGAG ATCATCGACC CCTTCGTGGA GGTGGAGGTC 
ATCGGGCTTC CCGTGGATTG CAACAGGGAG CAGACGCGTG TGGTGGATGA CAATGGATTC 
AACCCCATGT GGGAAGAGAC CCTGGTGTTC ACTGTGCACA TGCCTGAGAT CGCACTGGTG 
CGCTTCCTCG TCTGGGACCA TGACCCCATT GGGCGTGACT TCATTGGCCA GAGGACACTG 
GCCTTCAGCA GCATGATGCC AGGCTATCGG CACGTGTACC TAGAGGGGAT GGAAGAGGCC 
TCCATCTTTG TCCATGTGGC CGTCAGTGAC ATCAGTGGTA AGGTCAAGCA GGCTCTGGGC 
CTAAAAGGCC TGTTCCTCAG AGGCACAAAG CCCGGCTCCC TGGACAGTCA TGCTGCTGGG 
CGGCCCCTGC CCCGGCCTTC CGTTAGCCAG CGGCTCCTGC GGCGCACGGC CAGCGCCCCG 
ACCAAGAGCC AGAAACCCAG CCGCAAGGCC TTCCCAGAGC TGGTCCTGGG CACGCAGGAC 
ACGGGCTCCG AGGGGGAGGC CCGTGACGTG GCCGCCCCCA GCCCTGGCCC CGTCCTAGAG 
GCCTCAGCCC CAGAGGAGCC CGGCAGCCGC AGCCCCCGAG ATACCCGCCC CTTCTCCATG 
CAGAGGACGG GCTCCTCTCT ATGTGGCCTG GAAACTATTG CTGAGGAGCC AGCCCTGGGC 
CCCGGCCCCC CACCTCTGGT GGCTGCCCCC CCCAGCCCCT CCCCGGGAGG GCCCCTGTGC 
GCCTCGGGAC CTGGCACCAA AGTGACGAGC CCCCCAGCGG TCACTCTGGG AGCTTCCGTG 
CCCTTCCAGC CCAGGACCCG GAGCAGAGGG GACACTGAGC AGCCCCCGGA AAGCCGGCGG 
CAGGGTTACC ATGGTGGGGG GCCTGGAGGT GTGTGTGAGG GGACCCCTAG CAGCCAGGTG 
GTCAGAAGGG CCGAGAGTGA GGGGCAGGTG CCCGCGGAGC ACCCGGGTGG ATGGCGGCCC 
CCAGCCGGGC CCTGCCCAGC CGTGTACTCA GACGCCACAG GTGGAGACCG GCTGTGGCGG 
CGGCTGGAAC CTGGTGGCCA TCGCGACAGC GTGTCCTCGT CCTCCAGTGT GTCATCCAGC 
GACACGGTCA TCGACCTCTC CCTGCCGGGC CTGGGTCTGG GCCTCGTCCT GGGCCGTGAG 
GGTGTCTCGG GGGGTGTCTC GGGGGCTCCA GCTGGCCGCC TGCCCCTGCG GCCCTGCCCA 
GCCACTGCCG CCCGCCTGGA CCTGCCTGCC GTGACCAAGA GCAAATCCAG CCCCAACCTG 
CGGGCCGCCA GCCAGCTGCC CGCTGCACCA GAAGAGCTTC GGCTGCGTCC CCTGGTGCCC 
AGGCTGCCCT GGGGCCACCT TCCCCTGGCA GGCCTCCGGG ACTGCCCTGC AGCCGCCAAG 
TCTAAGAGCC TGGGGGACCT GACTGCCGAC GACTTTGCCC CCCAGGTGGA GATCCTGGGC 
CGAAGCCTGG GCCTGGCGAG GGAGGGGCGG GCAGGGCGGG GGGCGCGGCG GGACGCCCTG 
ACGGAACAGC TGCGCTGGCT CACAGGCTTC CAGCAGGCCG GTGACATCAC CTCGCCCACC 
AGCCTGAGTG TCGCTGGGGA TGGGGTGCCT GGTCCCCCTG GCTTCCTGCG GCGCTCCTCC 
TCCCGCAGCC AGAGCCGCGT GCGTGCCATT GCCAGCCGGG CCAGGCAGGC CCAGGAGCGG 
CAGCAACGGC TGCAGAGCCC GAGGGCGCCC CCCGGGGAGG AGCGGGGCAC CCCCGAGGGT 
GCCTGCTCAG GGGGCCAAGG GGGCTGCGGG GACGTGCCGG GCATGGGCCC CTCCAAGGGC 
TCCGCTGCCC CCGGCCTCCT GCTCAGACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_014113866.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969341.1 Canis lupus familiaris phospholipase C, eta 2 (PLCH2), transcript variant X1, mRNA.

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CloneID	OCe36624
Clone ID Related Accession (Same CDS sequence)	XM_014113866.1 , XM_014113866.2
Accession Version	XM_014113866.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC CCGGGGACCC TGGCGGGCTC AGTCGGGACG AAGTGGAGGG ATGCATGAGT 
GCCATGCAGG CGGGGACACA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG CCTGGTCCGC 
TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG 
AAGGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGCG AGGGTCGGCA GTCGGAGATC 
TTCCAGCGCT ACCCCGATGG CATTTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACCGAG AGTCGCTGGA CCTGGTCTCA CCCAGCGGGG ACGAGGCGCG CACCTGGGTC 
ACCGGCCTGC GCTACCTCAT GGCAGGCATC CGCGATGAGG ACAGCCTGGC CCGCCGCCAG 
CGCACTAGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCGGTGAGGT CCTGCAGCTG CTGCATAAGC TGAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AGCAGATGTT CAAGGAAGCA GATACAGATG ACCACCAGGG CACACTGGGC 
TTTGAGGAGT TCTGTGCCTT CTATAAGATG ATGTCCACCC GCCGGGACCT CTACCTGCTC 
ATGCTGACCT ACAGTGACCA CAAGGACCAC CTGAACGCCA CTGACCTCCA GCGCTTCCTG 
GAGGTGGAGC AGAAGATGGC GGGTGTGACC CTTGAGAGCT GCCGGGACAT CATCGAGCAG 
TTTGAGCCCT GCCCAGAAAA CAAGAGTAAG GGGGTGCTGG GCATTGATGG CTTCACCAAC 
TATACTCGGA GTCCCGCCGG CGACATCTTT AACCCAGAGC ACCATGGCGT GCACCAGGAC 
ATGACACGGC CGCTAAGCCA CTACTTCATC ACCTCATCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTAGAC ATGTACGCCT GGGTCCTGCA GGCCGGCTGC 
CGTTGCGTGG AGGTGGACTG CTGGGATGGG CCTGACGGGG AGCCCATTGT GCACCATGGC 
TATACTCTGA CCTCCAAGAT CCTCTTCAGA GATGTCATTG AAACCATCAA CAAATATGCT 
TTTGTCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAA 
CAGAAGAAGA TGGCCCAGTA TCTAACTGAT ATTCTTGGAG ATAAGCTGGA CGTGTCATCA 
GTAAGCAGTG ACGATGCCAC CTTGCTTCCT TCTCCACAGA TGCTCAAGGG AAAGATTCTG 
GTGAAGGGCA AGAAGCTTCC TGCCAACATC AGTGAGGATG CTGAAGAGGG CGAGGTGTCT 
GACGAAGACA GTGCAGATGA GATTGACGAG GACTGCAAAC TTCTCAATGG GGATGTTGCC 
ACCAACCGGA AGCGTGTAGA AAACATTGCC AAGAAGAAAC TGGATTCCCT GATGAAGGAG 
TCAAAGATTC GGGACTGCGA GGACCCAAAT GACTTCACTG TGTCCACGCT GCCCCCGTCT 
GGAAAGCTTG GGTACAAGGC AGAAGGCAAA AAGGCTGAGG AGGATGTGGA GTCCGGGGAG 
GACGCCGGGG TCAGCAGACG GAACAACCGG ATCCTCATGA GCGGTTTCTC CAAGCGCAAG 
AAGAAGAGCA GCAAGCTAAA GAAGGCGGCC AGCATGGAGG AGGGGGATGA GGACCTTGAC 
TCCCAGGGCA GTCAGAGTCG AGGGGCAAGC CGACAGAAGA AGACCATGAA GCTGTCCCGT 
GCCCTCTCGG ACCTGGTGAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGGCAGAG 
GTGGCGTCCA GCTGGCAGGT GTCGTCCTTC AGTGAGACCA GGGCCCAGCA GATCCTGCAG 
CAGAAGCCGG CACAGTACCT GCGCTTCAAC CAGCACCAGC TCTCCCGCAT CTACCCCTCC 
TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC AGGCTGCCAG 
ATGGTCGCCC TGAACTACCA GTCAGAGGGG CGGATGCTGC AGCTGAACCG CGCCAAGTTC 
AGTGCCAATG GGAACTGTGG CTATGTGCTC AAGCCCCAGT GCATGTGCCA GGGTGTCTTC 
AACCCCAACT CCGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTTCGGATC 
ATCAGTGGGC AGCAGCTCCC CAAGCCAAGG GACTCGATGC TGGGAGACCG AGGCGAGATC 
ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTTCCCG TGGATTGCAA CAGGGAGCAG 
ACGCGTGTGG TGGATGACAA TGGATTCAAC CCCATGTGGG AAGAGACCCT GGTGTTCACT 
GTGCACATGC CTGAGATCGC ACTGGTGCGC TTCCTCGTCT GGGACCATGA CCCCATTGGG 
CGTGACTTCA TTGGCCAGAG GACACTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC 
GTGTACCTAG AGGGGATGGA AGAGGCCTCC ATCTTTGTCC ATGTGGCCGT CAGTGACATC 
AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCAGAGG CACAAAGCCC 
GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCTTCCGT TAGCCAGCGG 
CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGCCAGA AACCCAGCCG CAAGGCCTTC 
CCAGAGCTGG TCCTGGGCAC GCAGGACACG GGCTCCGAGG GGGAGGCCCG TGACGTGGCC 
GCCCCCAGCC CTGGCCCCGT CCTAGAGGCC TCAGCCCCAG AGGAGCCCGG CAGCCGCAGC 
CCCCGAGATA CCCGCCCCTT CTCCATGCAG AGGACGGGCT CCTCTCTATG TGGCCTGGAA 
ACTATTGCTG AGGAGCCAGC CCTGGGCCCC GGCCCCCCAC CTCTGGTGGC TGCCCCCCCC 
AGCCCCTCCC CGGGAGGGCC CCTGTGCGCC TCGGGACCTG GCACCAAAGT GACGAGCCCC 
CCAGCGGTCA CTCTGGGAGC TTCCGTGCCC TTCCAGCCCA GGACCCGGAG CAGAGGGGAC 
ACTGAGCAGC CCCCGGAAAG CCGGCGGCAG GGTTACCATG GTGGGGGGCC TGGAGGTGTG 
TGTGAGGGGA CCCCTAGCAG CCAGGTGGTC AGAAGGGCCG AGAGTGAGGG GCAGGTGCCC 
GCGGAGCACC CGGGTGGATG GCGGCCCCCA GCCGGGCCCT GCCCAGCCGT GTACTCAGAC 
GCCACAGGTG GAGACCGGCT GTGGCGGCGG CTGGAACCTG GTGGCCATCG CGACAGCGTG 
TCCTCGTCCT CCAGTGTGTC ATCCAGCGAC ACGGTCATCG ACCTCTCCCT GCCGGGCCTG 
GGTCTGGGCC TCGTCCTGGG CCGTGAGGGT GTCTCGGGGG GTGTCTCGGG GGCTCCAGCT 
GGCCGCCTGC CCCTGCGGCC CTGCCCAGCC ACTGCCGCCC GCCTGGACCT GCCTGCCGTG 
ACCAAGAGCA AATCCAGCCC CAACCTGCGG GCCGCCAGCC AGCTGCCCGC TGCACCAGAA 
GAGCTTCGGC TGCGTCCCCT GGTGCCCAGG CTGCCCTGGG GCCACCTTCC CCTGGCAGGC 
CTCCGGGACT GCCCTGCAGC CGCCAAGTCT AAGAGCCTGG GGGACCTGAC TGCCGACGAC 
TTTGCCCCCC AGGTGGAGAT CCTGGGCCGA AGCCTGGGCC TGGCGAGGGA GGGGCGGGCA 
GGGCGGGGGG CGCGGCGGGA CGCCCTGACG GAACAGCTGC GCTGGCTCAC AGGCTTCCAG 
CAGGCCGGTG ACATCACCTC GCCCACCAGC CTGAGTGTCG CTGGGGATGG GGTGCCTGGT 
CCCCCTGGCT TCCTGCGGCG CTCCTCCTCC CGCAGCCAGA GCCGCGTGCG TGCCATTGCC 
AGCCGGGCCA GGCAGGCCCA GGAGCGGCAG CAACGGCTGC AGAGCCCGAG GGCGCCCCCC 
GGGGAGGAGC GGGGCACCCC CGAGGGTGCC TGCTCAGGGG GCCAAGGGGG CTGCGGGGAC 
GTGCCGGGCA TGGGCCCCTC CAAGGGCTCC GCTGCCCCCG GCCTCCTGCT CAGACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969341.1
CDS	133..4272
Translation	MEEPGDPGGLSRDEVEGCMSAMQAGTQMVKLRGSSKGLVRFYFLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDGIFDPNCCFSIYHGSHRESLDLVSPSGDEARTWVTGLRYLMAGIRDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFKEADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSDHKDHLNATDLQRFLEVEQKMAGVTLESCRDIIEQFEPCPENKSKGVLGIDGFTNYTRSPAGDIFNPEHHGVHQDMTRPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFRDVIETINKYAFVKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDVSSVSSDDATLLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDEDCKLLNGDVATNRKRVENIAKKKLDSLMKESKIRDCEDPNDFTVSTLPPSGKLGYKAEGKKAEEDVESGEDAGVSRRNNRILMSGFSKRKKKSSKLKKAASMEEGDEDLDSQGSQSRGASRQKKTMKLSRALSDLVKYTKSVGTHDVEAEVASSWQVSSFSETRAQQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGNCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCNREQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGTKPGSLDSHAAGRPLPRPSVSQRLLRRTASAPTKSQKPSRKAFPELVLGTQDTGSEGEARDVAAPSPGPVLEASAPEEPGSRSPRDTRPFSMQRTGSSLCGLETIAEEPALGPGPPPLVAAPPSPSPGGPLCASGPGTKVTSPPAVTLGASVPFQPRTRSRGDTEQPPESRRQGYHGGGPGGVCEGTPSSQVVRRAESEGQVPAEHPGGWRPPAGPCPAVYSDATGGDRLWRRLEPGGHRDSVSSSSSVSSSDTVIDLSLPGLGLGLVLGREGVSGGVSGAPAGRLPLRPCPATAARLDLPAVTKSKSSPNLRAASQLPAAPEELRLRPLVPRLPWGHLPLAGLRDCPAAAKSKSLGDLTADDFAPQVEILGRSLGLAREGRAGRGARRDALTEQLRWLTGFQQAGDITSPTSLSVAGDGVPGPPGFLRRSSSRSQSRVRAIASRARQAQERQQRLQSPRAPPGEERGTPEGACSGGQGGCGDVPGMGPSKGSAAPGLLLRL

Target ORF information:

RefSeq Version	XM_014113866.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 2 (PLCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113866.1

ORF Insert Sequence:

ATGGAGGAAC CCGGGGACCC TGGCGGGCTC AGTCGGGACG AAGTGGAGGG ATGCATGAGT 
GCCATGCAGG CGGGGACACA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG CCTGGTCCGC 
TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG 
AAGGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGCG AGGGTCGGCA GTCGGAGATC 
TTCCAGCGCT ACCCCGATGG CATTTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACCGAG AGTCGCTGGA CCTGGTCTCA CCCAGCGGGG ACGAGGCGCG CACCTGGGTC 
ACCGGCCTGC GCTACCTCAT GGCAGGCATC CGCGATGAGG ACAGCCTGGC CCGCCGCCAG 
CGCACTAGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCGGTGAGGT CCTGCAGCTG CTGCATAAGC TGAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AGCAGATGTT CAAGGAAGCA GATACAGATG ACCACCAGGG CACACTGGGC 
TTTGAGGAGT TCTGTGCCTT CTATAAGATG ATGTCCACCC GCCGGGACCT CTACCTGCTC 
ATGCTGACCT ACAGTGACCA CAAGGACCAC CTGAACGCCA CTGACCTCCA GCGCTTCCTG 
GAGGTGGAGC AGAAGATGGC GGGTGTGACC CTTGAGAGCT GCCGGGACAT CATCGAGCAG 
TTTGAGCCCT GCCCAGAAAA CAAGAGTAAG GGGGTGCTGG GCATTGATGG CTTCACCAAC 
TATACTCGGA GTCCCGCCGG CGACATCTTT AACCCAGAGC ACCATGGCGT GCACCAGGAC 
ATGACACGGC CGCTAAGCCA CTACTTCATC ACCTCATCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTAGAC ATGTACGCCT GGGTCCTGCA GGCCGGCTGC 
CGTTGCGTGG AGGTGGACTG CTGGGATGGG CCTGACGGGG AGCCCATTGT GCACCATGGC 
TATACTCTGA CCTCCAAGAT CCTCTTCAGA GATGTCATTG AAACCATCAA CAAATATGCT 
TTTGTCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAA 
CAGAAGAAGA TGGCCCAGTA TCTAACTGAT ATTCTTGGAG ATAAGCTGGA CGTGTCATCA 
GTAAGCAGTG ACGATGCCAC CTTGCTTCCT TCTCCACAGA TGCTCAAGGG AAAGATTCTG 
GTGAAGGGCA AGAAGCTTCC TGCCAACATC AGTGAGGATG CTGAAGAGGG CGAGGTGTCT 
GACGAAGACA GTGCAGATGA GATTGACGAG GACTGCAAAC TTCTCAATGG GGATGTTGCC 
ACCAACCGGA AGCGTGTAGA AAACATTGCC AAGAAGAAAC TGGATTCCCT GATGAAGGAG 
TCAAAGATTC GGGACTGCGA GGACCCAAAT GACTTCACTG TGTCCACGCT GCCCCCGTCT 
GGAAAGCTTG GGTACAAGGC AGAAGGCAAA AAGGCTGAGG AGGATGTGGA GTCCGGGGAG 
GACGCCGGGG TCAGCAGACG GAACAACCGG ATCCTCATGA GCGGTTTCTC CAAGCGCAAG 
AAGAAGAGCA GCAAGCTAAA GAAGGCGGCC AGCATGGAGG AGGGGGATGA GGACCTTGAC 
TCCCAGGGCA GTCAGAGTCG AGGGGCAAGC CGACAGAAGA AGACCATGAA GCTGTCCCGT 
GCCCTCTCGG ACCTGGTGAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGGCAGAG 
GTGGCGTCCA GCTGGCAGGT GTCGTCCTTC AGTGAGACCA GGGCCCAGCA GATCCTGCAG 
CAGAAGCCGG CACAGTACCT GCGCTTCAAC CAGCACCAGC TCTCCCGCAT CTACCCCTCC 
TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC AGGCTGCCAG 
ATGGTCGCCC TGAACTACCA GTCAGAGGGG CGGATGCTGC AGCTGAACCG CGCCAAGTTC 
AGTGCCAATG GGAACTGTGG CTATGTGCTC AAGCCCCAGT GCATGTGCCA GGGTGTCTTC 
AACCCCAACT CCGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTTCGGATC 
ATCAGTGGGC AGCAGCTCCC CAAGCCAAGG GACTCGATGC TGGGAGACCG AGGCGAGATC 
ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTTCCCG TGGATTGCAA CAGGGAGCAG 
ACGCGTGTGG TGGATGACAA TGGATTCAAC CCCATGTGGG AAGAGACCCT GGTGTTCACT 
GTGCACATGC CTGAGATCGC ACTGGTGCGC TTCCTCGTCT GGGACCATGA CCCCATTGGG 
CGTGACTTCA TTGGCCAGAG GACACTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC 
GTGTACCTAG AGGGGATGGA AGAGGCCTCC ATCTTTGTCC ATGTGGCCGT CAGTGACATC 
AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCAGAGG CACAAAGCCC 
GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCTTCCGT TAGCCAGCGG 
CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGCCAGA AACCCAGCCG CAAGGCCTTC 
CCAGAGCTGG TCCTGGGCAC GCAGGACACG GGCTCCGAGG GGGAGGCCCG TGACGTGGCC 
GCCCCCAGCC CTGGCCCCGT CCTAGAGGCC TCAGCCCCAG AGGAGCCCGG CAGCCGCAGC 
CCCCGAGATA CCCGCCCCTT CTCCATGCAG AGGACGGGCT CCTCTCTATG TGGCCTGGAA 
ACTATTGCTG AGGAGCCAGC CCTGGGCCCC GGCCCCCCAC CTCTGGTGGC TGCCCCCCCC 
AGCCCCTCCC CGGGAGGGCC CCTGTGCGCC TCGGGACCTG GCACCAAAGT GACGAGCCCC 
CCAGCGGTCA CTCTGGGAGC TTCCGTGCCC TTCCAGCCCA GGACCCGGAG CAGAGGGGAC 
ACTGAGCAGC CCCCGGAAAG CCGGCGGCAG GGTTACCATG GTGGGGGGCC TGGAGGTGTG 
TGTGAGGGGA CCCCTAGCAG CCAGGTGGTC AGAAGGGCCG AGAGTGAGGG GCAGGTGCCC 
GCGGAGCACC CGGGTGGATG GCGGCCCCCA GCCGGGCCCT GCCCAGCCGT GTACTCAGAC 
GCCACAGGTG GAGACCGGCT GTGGCGGCGG CTGGAACCTG GTGGCCATCG CGACAGCGTG 
TCCTCGTCCT CCAGTGTGTC ATCCAGCGAC ACGGTCATCG ACCTCTCCCT GCCGGGCCTG 
GGTCTGGGCC TCGTCCTGGG CCGTGAGGGT GTCTCGGGGG GTGTCTCGGG GGCTCCAGCT 
GGCCGCCTGC CCCTGCGGCC CTGCCCAGCC ACTGCCGCCC GCCTGGACCT GCCTGCCGTG 
ACCAAGAGCA AATCCAGCCC CAACCTGCGG GCCGCCAGCC AGCTGCCCGC TGCACCAGAA 
GAGCTTCGGC TGCGTCCCCT GGTGCCCAGG CTGCCCTGGG GCCACCTTCC CCTGGCAGGC 
CTCCGGGACT GCCCTGCAGC CGCCAAGTCT AAGAGCCTGG GGGACCTGAC TGCCGACGAC 
TTTGCCCCCC AGGTGGAGAT CCTGGGCCGA AGCCTGGGCC TGGCGAGGGA GGGGCGGGCA 
GGGCGGGGGG CGCGGCGGGA CGCCCTGACG GAACAGCTGC GCTGGCTCAC AGGCTTCCAG 
CAGGCCGGTG ACATCACCTC GCCCACCAGC CTGAGTGTCG CTGGGGATGG GGTGCCTGGT 
CCCCCTGGCT TCCTGCGGCG CTCCTCCTCC CGCAGCCAGA GCCGCGTGCG TGCCATTGCC 
AGCCGGGCCA GGCAGGCCCA GGAGCGGCAG CAACGGCTGC AGAGCCCGAG GGCGCCCCCC 
GGGGAGGAGC GGGGCACCCC CGAGGGTGCC TGCTCAGGGG GCCAAGGGGG CTGCGGGGAC 
GTGCCGGGCA TGGGCCCCTC CAAGGGCTCC GCTGCCCCCG GCCTCCTGCT CAGACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_014113866.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969341.1 Canis lupus familiaris phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.

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CloneID	OCe36624
Clone ID Related Accession (Same CDS sequence)	XM_014113866.1 , XM_014113866.2
Accession Version	XM_014113866.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC CCGGGGACCC TGGCGGGCTC AGTCGGGACG AAGTGGAGGG ATGCATGAGT 
GCCATGCAGG CGGGGACACA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG CCTGGTCCGC 
TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG 
AAGGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGCG AGGGTCGGCA GTCGGAGATC 
TTCCAGCGCT ACCCCGATGG CATTTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACCGAG AGTCGCTGGA CCTGGTCTCA CCCAGCGGGG ACGAGGCGCG CACCTGGGTC 
ACCGGCCTGC GCTACCTCAT GGCAGGCATC CGCGATGAGG ACAGCCTGGC CCGCCGCCAG 
CGCACTAGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCGGTGAGGT CCTGCAGCTG CTGCATAAGC TGAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AGCAGATGTT CAAGGAAGCA GATACAGATG ACCACCAGGG CACACTGGGC 
TTTGAGGAGT TCTGTGCCTT CTATAAGATG ATGTCCACCC GCCGGGACCT CTACCTGCTC 
ATGCTGACCT ACAGTGACCA CAAGGACCAC CTGAACGCCA CTGACCTCCA GCGCTTCCTG 
GAGGTGGAGC AGAAGATGGC GGGTGTGACC CTTGAGAGCT GCCGGGACAT CATCGAGCAG 
TTTGAGCCCT GCCCAGAAAA CAAGAGTAAG GGGGTGCTGG GCATTGATGG CTTCACCAAC 
TATACTCGGA GTCCCGCCGG CGACATCTTT AACCCAGAGC ACCATGGCGT GCACCAGGAC 
ATGACACGGC CGCTAAGCCA CTACTTCATC ACCTCATCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTAGAC ATGTACGCCT GGGTCCTGCA GGCCGGCTGC 
CGTTGCGTGG AGGTGGACTG CTGGGATGGG CCTGACGGGG AGCCCATTGT GCACCATGGC 
TATACTCTGA CCTCCAAGAT CCTCTTCAGA GATGTCATTG AAACCATCAA CAAATATGCT 
TTTGTCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAA 
CAGAAGAAGA TGGCCCAGTA TCTAACTGAT ATTCTTGGAG ATAAGCTGGA CGTGTCATCA 
GTAAGCAGTG ACGATGCCAC CTTGCTTCCT TCTCCACAGA TGCTCAAGGG AAAGATTCTG 
GTGAAGGGCA AGAAGCTTCC TGCCAACATC AGTGAGGATG CTGAAGAGGG CGAGGTGTCT 
GACGAAGACA GTGCAGATGA GATTGACGAG GACTGCAAAC TTCTCAATGG GGATGTTGCC 
ACCAACCGGA AGCGTGTAGA AAACATTGCC AAGAAGAAAC TGGATTCCCT GATGAAGGAG 
TCAAAGATTC GGGACTGCGA GGACCCAAAT GACTTCACTG TGTCCACGCT GCCCCCGTCT 
GGAAAGCTTG GGTACAAGGC AGAAGGCAAA AAGGCTGAGG AGGATGTGGA GTCCGGGGAG 
GACGCCGGGG TCAGCAGACG GAACAACCGG ATCCTCATGA GCGGTTTCTC CAAGCGCAAG 
AAGAAGAGCA GCAAGCTAAA GAAGGCGGCC AGCATGGAGG AGGGGGATGA GGACCTTGAC 
TCCCAGGGCA GTCAGAGTCG AGGGGCAAGC CGACAGAAGA AGACCATGAA GCTGTCCCGT 
GCCCTCTCGG ACCTGGTGAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGGCAGAG 
GTGGCGTCCA GCTGGCAGGT GTCGTCCTTC AGTGAGACCA GGGCCCAGCA GATCCTGCAG 
CAGAAGCCGG CACAGTACCT GCGCTTCAAC CAGCACCAGC TCTCCCGCAT CTACCCCTCC 
TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC AGGCTGCCAG 
ATGGTCGCCC TGAACTACCA GTCAGAGGGG CGGATGCTGC AGCTGAACCG CGCCAAGTTC 
AGTGCCAATG GGAACTGTGG CTATGTGCTC AAGCCCCAGT GCATGTGCCA GGGTGTCTTC 
AACCCCAACT CCGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTTCGGATC 
ATCAGTGGGC AGCAGCTCCC CAAGCCAAGG GACTCGATGC TGGGAGACCG AGGCGAGATC 
ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTTCCCG TGGATTGCAA CAGGGAGCAG 
ACGCGTGTGG TGGATGACAA TGGATTCAAC CCCATGTGGG AAGAGACCCT GGTGTTCACT 
GTGCACATGC CTGAGATCGC ACTGGTGCGC TTCCTCGTCT GGGACCATGA CCCCATTGGG 
CGTGACTTCA TTGGCCAGAG GACACTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC 
GTGTACCTAG AGGGGATGGA AGAGGCCTCC ATCTTTGTCC ATGTGGCCGT CAGTGACATC 
AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCAGAGG CACAAAGCCC 
GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCTTCCGT TAGCCAGCGG 
CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGCCAGA AACCCAGCCG CAAGGCCTTC 
CCAGAGCTGG TCCTGGGCAC GCAGGACACG GGCTCCGAGG GGGAGGCCCG TGACGTGGCC 
GCCCCCAGCC CTGGCCCCGT CCTAGAGGCC TCAGCCCCAG AGGAGCCCGG CAGCCGCAGC 
CCCCGAGATA CCCGCCCCTT CTCCATGCAG AGGACGGGCT CCTCTCTATG TGGCCTGGAA 
ACTATTGCTG AGGAGCCAGC CCTGGGCCCC GGCCCCCCAC CTCTGGTGGC TGCCCCCCCC 
AGCCCCTCCC CGGGAGGGCC CCTGTGCGCC TCGGGACCTG GCACCAAAGT GACGAGCCCC 
CCAGCGGTCA CTCTGGGAGC TTCCGTGCCC TTCCAGCCCA GGACCCGGAG CAGAGGGGAC 
ACTGAGCAGC CCCCGGAAAG CCGGCGGCAG GGTTACCATG GTGGGGGGCC TGGAGGTGTG 
TGTGAGGGGA CCCCTAGCAG CCAGGTGGTC AGAAGGGCCG AGAGTGAGGG GCAGGTGCCC 
GCGGAGCACC CGGGTGGATG GCGGCCCCCA GCCGGGCCCT GCCCAGCCGT GTACTCAGAC 
GCCACAGGTG GAGACCGGCT GTGGCGGCGG CTGGAACCTG GTGGCCATCG CGACAGCGTG 
TCCTCGTCCT CCAGTGTGTC ATCCAGCGAC ACGGTCATCG ACCTCTCCCT GCCGGGCCTG 
GGTCTGGGCC TCGTCCTGGG CCGTGAGGGT GTCTCGGGGG GTGTCTCGGG GGCTCCAGCT 
GGCCGCCTGC CCCTGCGGCC CTGCCCAGCC ACTGCCGCCC GCCTGGACCT GCCTGCCGTG 
ACCAAGAGCA AATCCAGCCC CAACCTGCGG GCCGCCAGCC AGCTGCCCGC TGCACCAGAA 
GAGCTTCGGC TGCGTCCCCT GGTGCCCAGG CTGCCCTGGG GCCACCTTCC CCTGGCAGGC 
CTCCGGGACT GCCCTGCAGC CGCCAAGTCT AAGAGCCTGG GGGACCTGAC TGCCGACGAC 
TTTGCCCCCC AGGTGGAGAT CCTGGGCCGA AGCCTGGGCC TGGCGAGGGA GGGGCGGGCA 
GGGCGGGGGG CGCGGCGGGA CGCCCTGACG GAACAGCTGC GCTGGCTCAC AGGCTTCCAG 
CAGGCCGGTG ACATCACCTC GCCCACCAGC CTGAGTGTCG CTGGGGATGG GGTGCCTGGT 
CCCCCTGGCT TCCTGCGGCG CTCCTCCTCC CGCAGCCAGA GCCGCGTGCG TGCCATTGCC 
AGCCGGGCCA GGCAGGCCCA GGAGCGGCAG CAACGGCTGC AGAGCCCGAG GGCGCCCCCC 
GGGGAGGAGC GGGGCACCCC CGAGGGTGCC TGCTCAGGGG GCCAAGGGGG CTGCGGGGAC 
GTGCCGGGCA TGGGCCCCTC CAAGGGCTCC GCTGCCCCCG GCCTCCTGCT CAGACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969341.1
CDS	137..4276
Translation	MEEPGDPGGLSRDEVEGCMSAMQAGTQMVKLRGSSKGLVRFYFLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDGIFDPNCCFSIYHGSHRESLDLVSPSGDEARTWVTGLRYLMAGIRDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFKEADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSDHKDHLNATDLQRFLEVEQKMAGVTLESCRDIIEQFEPCPENKSKGVLGIDGFTNYTRSPAGDIFNPEHHGVHQDMTRPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFRDVIETINKYAFVKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDVSSVSSDDATLLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDEDCKLLNGDVATNRKRVENIAKKKLDSLMKESKIRDCEDPNDFTVSTLPPSGKLGYKAEGKKAEEDVESGEDAGVSRRNNRILMSGFSKRKKKSSKLKKAASMEEGDEDLDSQGSQSRGASRQKKTMKLSRALSDLVKYTKSVGTHDVEAEVASSWQVSSFSETRAQQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGNCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCNREQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGTKPGSLDSHAAGRPLPRPSVSQRLLRRTASAPTKSQKPSRKAFPELVLGTQDTGSEGEARDVAAPSPGPVLEASAPEEPGSRSPRDTRPFSMQRTGSSLCGLETIAEEPALGPGPPPLVAAPPSPSPGGPLCASGPGTKVTSPPAVTLGASVPFQPRTRSRGDTEQPPESRRQGYHGGGPGGVCEGTPSSQVVRRAESEGQVPAEHPGGWRPPAGPCPAVYSDATGGDRLWRRLEPGGHRDSVSSSSSVSSSDTVIDLSLPGLGLGLVLGREGVSGGVSGAPAGRLPLRPCPATAARLDLPAVTKSKSSPNLRAASQLPAAPEELRLRPLVPRLPWGHLPLAGLRDCPAAAKSKSLGDLTADDFAPQVEILGRSLGLAREGRAGRGARRDALTEQLRWLTGFQQAGDITSPTSLSVAGDGVPGPPGFLRRSSSRSQSRVRAIASRARQAQERQQRLQSPRAPPGEERGTPEGACSGGQGGCGDVPGMGPSKGSAAPGLLLRL

Target ORF information:

RefSeq Version	XM_014113866.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113866.2

ORF Insert Sequence:

ATGGAGGAAC CCGGGGACCC TGGCGGGCTC AGTCGGGACG AAGTGGAGGG ATGCATGAGT 
GCCATGCAGG CGGGGACACA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG CCTGGTCCGC 
TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG 
AAGGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGCG AGGGTCGGCA GTCGGAGATC 
TTCCAGCGCT ACCCCGATGG CATTTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACCGAG AGTCGCTGGA CCTGGTCTCA CCCAGCGGGG ACGAGGCGCG CACCTGGGTC 
ACCGGCCTGC GCTACCTCAT GGCAGGCATC CGCGATGAGG ACAGCCTGGC CCGCCGCCAG 
CGCACTAGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCGGTGAGGT CCTGCAGCTG CTGCATAAGC TGAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AGCAGATGTT CAAGGAAGCA GATACAGATG ACCACCAGGG CACACTGGGC 
TTTGAGGAGT TCTGTGCCTT CTATAAGATG ATGTCCACCC GCCGGGACCT CTACCTGCTC 
ATGCTGACCT ACAGTGACCA CAAGGACCAC CTGAACGCCA CTGACCTCCA GCGCTTCCTG 
GAGGTGGAGC AGAAGATGGC GGGTGTGACC CTTGAGAGCT GCCGGGACAT CATCGAGCAG 
TTTGAGCCCT GCCCAGAAAA CAAGAGTAAG GGGGTGCTGG GCATTGATGG CTTCACCAAC 
TATACTCGGA GTCCCGCCGG CGACATCTTT AACCCAGAGC ACCATGGCGT GCACCAGGAC 
ATGACACGGC CGCTAAGCCA CTACTTCATC ACCTCATCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTAGAC ATGTACGCCT GGGTCCTGCA GGCCGGCTGC 
CGTTGCGTGG AGGTGGACTG CTGGGATGGG CCTGACGGGG AGCCCATTGT GCACCATGGC 
TATACTCTGA CCTCCAAGAT CCTCTTCAGA GATGTCATTG AAACCATCAA CAAATATGCT 
TTTGTCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAA 
CAGAAGAAGA TGGCCCAGTA TCTAACTGAT ATTCTTGGAG ATAAGCTGGA CGTGTCATCA 
GTAAGCAGTG ACGATGCCAC CTTGCTTCCT TCTCCACAGA TGCTCAAGGG AAAGATTCTG 
GTGAAGGGCA AGAAGCTTCC TGCCAACATC AGTGAGGATG CTGAAGAGGG CGAGGTGTCT 
GACGAAGACA GTGCAGATGA GATTGACGAG GACTGCAAAC TTCTCAATGG GGATGTTGCC 
ACCAACCGGA AGCGTGTAGA AAACATTGCC AAGAAGAAAC TGGATTCCCT GATGAAGGAG 
TCAAAGATTC GGGACTGCGA GGACCCAAAT GACTTCACTG TGTCCACGCT GCCCCCGTCT 
GGAAAGCTTG GGTACAAGGC AGAAGGCAAA AAGGCTGAGG AGGATGTGGA GTCCGGGGAG 
GACGCCGGGG TCAGCAGACG GAACAACCGG ATCCTCATGA GCGGTTTCTC CAAGCGCAAG 
AAGAAGAGCA GCAAGCTAAA GAAGGCGGCC AGCATGGAGG AGGGGGATGA GGACCTTGAC 
TCCCAGGGCA GTCAGAGTCG AGGGGCAAGC CGACAGAAGA AGACCATGAA GCTGTCCCGT 
GCCCTCTCGG ACCTGGTGAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGGCAGAG 
GTGGCGTCCA GCTGGCAGGT GTCGTCCTTC AGTGAGACCA GGGCCCAGCA GATCCTGCAG 
CAGAAGCCGG CACAGTACCT GCGCTTCAAC CAGCACCAGC TCTCCCGCAT CTACCCCTCC 
TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC AGGCTGCCAG 
ATGGTCGCCC TGAACTACCA GTCAGAGGGG CGGATGCTGC AGCTGAACCG CGCCAAGTTC 
AGTGCCAATG GGAACTGTGG CTATGTGCTC AAGCCCCAGT GCATGTGCCA GGGTGTCTTC 
AACCCCAACT CCGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTTCGGATC 
ATCAGTGGGC AGCAGCTCCC CAAGCCAAGG GACTCGATGC TGGGAGACCG AGGCGAGATC 
ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTTCCCG TGGATTGCAA CAGGGAGCAG 
ACGCGTGTGG TGGATGACAA TGGATTCAAC CCCATGTGGG AAGAGACCCT GGTGTTCACT 
GTGCACATGC CTGAGATCGC ACTGGTGCGC TTCCTCGTCT GGGACCATGA CCCCATTGGG 
CGTGACTTCA TTGGCCAGAG GACACTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC 
GTGTACCTAG AGGGGATGGA AGAGGCCTCC ATCTTTGTCC ATGTGGCCGT CAGTGACATC 
AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCAGAGG CACAAAGCCC 
GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCTTCCGT TAGCCAGCGG 
CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGCCAGA AACCCAGCCG CAAGGCCTTC 
CCAGAGCTGG TCCTGGGCAC GCAGGACACG GGCTCCGAGG GGGAGGCCCG TGACGTGGCC 
GCCCCCAGCC CTGGCCCCGT CCTAGAGGCC TCAGCCCCAG AGGAGCCCGG CAGCCGCAGC 
CCCCGAGATA CCCGCCCCTT CTCCATGCAG AGGACGGGCT CCTCTCTATG TGGCCTGGAA 
ACTATTGCTG AGGAGCCAGC CCTGGGCCCC GGCCCCCCAC CTCTGGTGGC TGCCCCCCCC 
AGCCCCTCCC CGGGAGGGCC CCTGTGCGCC TCGGGACCTG GCACCAAAGT GACGAGCCCC 
CCAGCGGTCA CTCTGGGAGC TTCCGTGCCC TTCCAGCCCA GGACCCGGAG CAGAGGGGAC 
ACTGAGCAGC CCCCGGAAAG CCGGCGGCAG GGTTACCATG GTGGGGGGCC TGGAGGTGTG 
TGTGAGGGGA CCCCTAGCAG CCAGGTGGTC AGAAGGGCCG AGAGTGAGGG GCAGGTGCCC 
GCGGAGCACC CGGGTGGATG GCGGCCCCCA GCCGGGCCCT GCCCAGCCGT GTACTCAGAC 
GCCACAGGTG GAGACCGGCT GTGGCGGCGG CTGGAACCTG GTGGCCATCG CGACAGCGTG 
TCCTCGTCCT CCAGTGTGTC ATCCAGCGAC ACGGTCATCG ACCTCTCCCT GCCGGGCCTG 
GGTCTGGGCC TCGTCCTGGG CCGTGAGGGT GTCTCGGGGG GTGTCTCGGG GGCTCCAGCT 
GGCCGCCTGC CCCTGCGGCC CTGCCCAGCC ACTGCCGCCC GCCTGGACCT GCCTGCCGTG 
ACCAAGAGCA AATCCAGCCC CAACCTGCGG GCCGCCAGCC AGCTGCCCGC TGCACCAGAA 
GAGCTTCGGC TGCGTCCCCT GGTGCCCAGG CTGCCCTGGG GCCACCTTCC CCTGGCAGGC 
CTCCGGGACT GCCCTGCAGC CGCCAAGTCT AAGAGCCTGG GGGACCTGAC TGCCGACGAC 
TTTGCCCCCC AGGTGGAGAT CCTGGGCCGA AGCCTGGGCC TGGCGAGGGA GGGGCGGGCA 
GGGCGGGGGG CGCGGCGGGA CGCCCTGACG GAACAGCTGC GCTGGCTCAC AGGCTTCCAG 
CAGGCCGGTG ACATCACCTC GCCCACCAGC CTGAGTGTCG CTGGGGATGG GGTGCCTGGT 
CCCCCTGGCT TCCTGCGGCG CTCCTCCTCC CGCAGCCAGA GCCGCGTGCG TGCCATTGCC 
AGCCGGGCCA GGCAGGCCCA GGAGCGGCAG CAACGGCTGC AGAGCCCGAG GGCGCCCCCC 
GGGGAGGAGC GGGGCACCCC CGAGGGTGCC TGCTCAGGGG GCCAAGGGGG CTGCGGGGAC 
GTGCCGGGCA TGGGCCCCTC CAAGGGCTCC GCTGCCCCCG GCCTCCTGCT CAGACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_014113867.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969342.1 Canis lupus familiaris phospholipase C, eta 2 (PLCH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe36625
Clone ID Related Accession (Same CDS sequence)	XM_014113867.1
Accession Version	XM_014113867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGG GAGCCGCCCC GCGGAAGCAC ATGGAGGGAT GCATGAGTGC CATGCAGGCG 
GGGACACAGA TGGTGAAGCT CCGTGGCAGC TCCAAGGGCC TGGTCCGCTT CTACTTCCTG 
GACGAGCATC GCTCCTGCAT CCGCTGGCGG CCCTCACGCA AGAATGAGAA GGCCAAGATT 
TCCATCGACT CCATCCAGGA GGTGAGCGAG GGTCGGCAGT CGGAGATCTT CCAGCGCTAC 
CCCGATGGCA TTTTTGACCC CAACTGCTGC TTCAGCATCT ACCATGGCAG CCACCGAGAG 
TCGCTGGACC TGGTCTCACC CAGCGGGGAC GAGGCGCGCA CCTGGGTCAC CGGCCTGCGC 
TACCTCATGG CAGGCATCCG CGATGAGGAC AGCCTGGCCC GCCGCCAGCG CACTAGGGAC 
CAGTGGCTGA AGCAGACATT TGACGAGGCC GACAAGAACG GGGACGGCAG CCTGAGCATC 
GGTGAGGTCC TGCAGCTGCT GCATAAGCTG AACGTGAACC TGCCCCGGCA GAGGGTGAAG 
CAGATGTTCA AGGAAGCAGA TACAGATGAC CACCAGGGCA CACTGGGCTT TGAGGAGTTC 
TGTGCCTTCT ATAAGATGAT GTCCACCCGC CGGGACCTCT ACCTGCTCAT GCTGACCTAC 
AGTGACCACA AGGACCACCT GAACGCCACT GACCTCCAGC GCTTCCTGGA GGTGGAGCAG 
AAGATGGCGG GTGTGACCCT TGAGAGCTGC CGGGACATCA TCGAGCAGTT TGAGCCCTGC 
CCAGAAAACA AGAGTAAGGG GGTGCTGGGC ATTGATGGCT TCACCAACTA TACTCGGAGT 
CCCGCCGGCG ACATCTTTAA CCCAGAGCAC CATGGCGTGC ACCAGGACAT GACACGGCCG 
CTAAGCCACT ACTTCATCAC CTCATCCCAC AACACCTACC TCGTGGGTGA CCAGCTCATG 
TCCCAGTCTC GGGTAGACAT GTACGCCTGG GTCCTGCAGG CCGGCTGCCG TTGCGTGGAG 
GTGGACTGCT GGGATGGGCC TGACGGGGAG CCCATTGTGC ACCATGGCTA TACTCTGACC 
TCCAAGATCC TCTTCAGAGA TGTCATTGAA ACCATCAACA AATATGCTTT TGTCAAGAAT 
GAGTACCCAG TGATCCTGTC CATTGAGAAC CACTGCAGTG TCATCCAACA GAAGAAGATG 
GCCCAGTATC TAACTGATAT TCTTGGAGAT AAGCTGGACG TGTCATCAGT AAGCAGTGAC 
GATGCCACCT TGCTTCCTTC TCCACAGATG CTCAAGGGAA AGATTCTGGT GAAGGGCAAG 
AAGCTTCCTG CCAACATCAG TGAGGATGCT GAAGAGGGCG AGGTGTCTGA CGAAGACAGT 
GCAGATGAGA TTGACGAGGA CTGCAAACTT CTCAATGGGG ATGTTGCCAC CAACCGGAAG 
CGTGTAGAAA ACATTGCCAA GAAGAAACTG GATTCCCTGA TGAAGGAGTC AAAGATTCGG 
GACTGCGAGG ACCCAAATGA CTTCACTGTG TCCACGCTGC CCCCGTCTGG AAAGCTTGGG 
TACAAGGCAG AAGGCAAAAA GGCTGAGGAG GATGTGGAGT CCGGGGAGGA CGCCGGGGTC 
AGCAGACGGA ACAACCGGAT CCTCATGAGC GGTTTCTCCA AGCGCAAGAA GAAGAGCAGC 
AAGCTAAAGA AGGCGGCCAG CATGGAGGAG GGGGATGAGG ACCTTGACTC CCAGGGCAGT 
CAGAGTCGAG GGGCAAGCCG ACAGAAGAAG ACCATGAAGC TGTCCCGTGC CCTCTCGGAC 
CTGGTGAAGT ACACCAAGTC TGTGGGCACC CACGACGTGG AGGCAGAGGT GGCGTCCAGC 
TGGCAGGTGT CGTCCTTCAG TGAGACCAGG GCCCAGCAGA TCCTGCAGCA GAAGCCGGCA 
CAGTACCTGC GCTTCAACCA GCACCAGCTC TCCCGCATCT ACCCCTCCTC CTACCGCGTG 
GACTCCAGCA ACTACAACCC GCAGCCCTTC TGGAACGCAG GCTGCCAGAT GGTCGCCCTG 
AACTACCAGT CAGAGGGGCG GATGCTGCAG CTGAACCGCG CCAAGTTCAG TGCCAATGGG 
AACTGTGGCT ATGTGCTCAA GCCCCAGTGC ATGTGCCAGG GTGTCTTCAA CCCCAACTCC 
GAGGACCCCC TGCCCGGGCA GCTCAAGAAG CAGCTGGTGC TTCGGATCAT CAGTGGGCAG 
CAGCTCCCCA AGCCAAGGGA CTCGATGCTG GGAGACCGAG GCGAGATCAT CGACCCCTTC 
GTGGAGGTGG AGGTCATCGG GCTTCCCGTG GATTGCAACA GGGAGCAGAC GCGTGTGGTG 
GATGACAATG GATTCAACCC CATGTGGGAA GAGACCCTGG TGTTCACTGT GCACATGCCT 
GAGATCGCAC TGGTGCGCTT CCTCGTCTGG GACCATGACC CCATTGGGCG TGACTTCATT 
GGCCAGAGGA CACTGGCCTT CAGCAGCATG ATGCCAGGCT ATCGGCACGT GTACCTAGAG 
GGGATGGAAG AGGCCTCCAT CTTTGTCCAT GTGGCCGTCA GTGACATCAG TGGTAAGGTC 
AAGCAGGCTC TGGGCCTAAA AGGCCTGTTC CTCAGAGGCA CAAAGCCCGG CTCCCTGGAC 
AGTCATGCTG CTGGGCGGCC CCTGCCCCGG CCTTCCGTTA GCCAGCGGCT CCTGCGGCGC 
ACGGCCAGCG CCCCGACCAA GAGCCAGAAA CCCAGCCGCA AGGCCTTCCC AGAGCTGGTC 
CTGGGCACGC AGGACACGGG CTCCGAGGGG GAGGCCCGTG ACGTGGCCGC CCCCAGCCCT 
GGCCCCGTCC TAGAGGCCTC AGCCCCAGAG GAGCCCGGCA GCCGCAGCCC CCGAGATACC 
CGCCCCTTCT CCATGCAGAG GACGGGCTCC TCTCTATGTG GCCTGGAAAC TATTGCTGAG 
GAGCCAGCCC TGGGCCCCGG CCCCCCACCT CTGGTGGCTG CCCCCCCCAG CCCCTCCCCG 
GGAGGGCCCC TGTGCGCCTC GGGACCTGGC ACCAAAGTGA CGAGCCCCCC AGCGGTCACT 
CTGGGAGCTT CCGTGCCCTT CCAGCCCAGG ACCCGGAGCA GAGGGGACAC TGAGCAGCCC 
CCGGAAAGCC GGCGGCAGGG TTACCATGGT GGGGGGCCTG GAGGTGTGTG TGAGGGGACC 
CCTAGCAGCC AGGTGGTCAG AAGGGCCGAG AGTGAGGGGC AGGTGCCCGC GGAGCACCCG 
GGTGGATGGC GGCCCCCAGC CGGGCCCTGC CCAGCCGTGT ACTCAGACGC CACAGGTGGA 
GACCGGCTGT GGCGGCGGCT GGAACCTGGT GGCCATCGCG ACAGCGTGTC CTCGTCCTCC 
AGTGTGTCAT CCAGCGACAC GGTCATCGAC CTCTCCCTGC CGGGCCTGGG TCTGGGCCTC 
GTCCTGGGCC GTGAGGGTGT CTCGGGGGGT GTCTCGGGGG CTCCAGCTGG CCGCCTGCCC 
CTGCGGCCCT GCCCAGCCAC TGCCGCCCGC CTGGACCTGC CTGCCGTGAC CAAGAGCAAA 
TCCAGCCCCA ACCTGCGGGC CGCCAGCCAG CTGCCCGCTG CACCAGAAGA GCTTCGGCTG 
CGTCCCCTGG TGCCCAGGCT GCCCTGGGGC CACCTTCCCC TGGCAGGCCT CCGGGACTGC 
CCTGCAGCCG CCAAGTCTAA GAGCCTGGGG GACCTGACTG CCGACGACTT TGCCCCCCAG 
GTGGAGATCC TGGGCCGAAG CCTGGGCCTG GCGAGGGAGG GGCGGGCAGG GCGGGGGGCG 
CGGCGGGACG CCCTGACGGA ACAGCTGCGC TGGCTCACAG GCTTCCAGCA GGCCGGTGAC 
ATCACCTCGC CCACCAGCCT GAGTGTCGCT GGGGATGGGG TGCCTGGTCC CCCTGGCTTC 
CTGCGGCGCT CCTCCTCCCG CAGCCAGAGC CGCGTGCGTG CCATTGCCAG CCGGGCCAGG 
CAGGCCCAGG AGCGGCAGCA ACGGCTGCAG AGCCCGAGGG CGCCCCCCGG GGAGGAGCGG 
GGCACCCCCG AGGGTGCCTG CTCAGGGGGC CAAGGGGGCT GCGGGGACGT GCCGGGCATG 
GGCCCCTCCA AGGGCTCCGC TGCCCCCGGC CTCCTGCTCA GACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969342.1
CDS	20..4147
Translation	MDAGAAPRKHMEGCMSAMQAGTQMVKLRGSSKGLVRFYFLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDGIFDPNCCFSIYHGSHRESLDLVSPSGDEARTWVTGLRYLMAGIRDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFKEADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSDHKDHLNATDLQRFLEVEQKMAGVTLESCRDIIEQFEPCPENKSKGVLGIDGFTNYTRSPAGDIFNPEHHGVHQDMTRPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFRDVIETINKYAFVKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDVSSVSSDDATLLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDEDCKLLNGDVATNRKRVENIAKKKLDSLMKESKIRDCEDPNDFTVSTLPPSGKLGYKAEGKKAEEDVESGEDAGVSRRNNRILMSGFSKRKKKSSKLKKAASMEEGDEDLDSQGSQSRGASRQKKTMKLSRALSDLVKYTKSVGTHDVEAEVASSWQVSSFSETRAQQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGNCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCNREQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGTKPGSLDSHAAGRPLPRPSVSQRLLRRTASAPTKSQKPSRKAFPELVLGTQDTGSEGEARDVAAPSPGPVLEASAPEEPGSRSPRDTRPFSMQRTGSSLCGLETIAEEPALGPGPPPLVAAPPSPSPGGPLCASGPGTKVTSPPAVTLGASVPFQPRTRSRGDTEQPPESRRQGYHGGGPGGVCEGTPSSQVVRRAESEGQVPAEHPGGWRPPAGPCPAVYSDATGGDRLWRRLEPGGHRDSVSSSSSVSSSDTVIDLSLPGLGLGLVLGREGVSGGVSGAPAGRLPLRPCPATAARLDLPAVTKSKSSPNLRAASQLPAAPEELRLRPLVPRLPWGHLPLAGLRDCPAAAKSKSLGDLTADDFAPQVEILGRSLGLAREGRAGRGARRDALTEQLRWLTGFQQAGDITSPTSLSVAGDGVPGPPGFLRRSSSRSQSRVRAIASRARQAQERQQRLQSPRAPPGEERGTPEGACSGGQGGCGDVPGMGPSKGSAAPGLLLRL

Target ORF information:

RefSeq Version	XM_014113867.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 2 (PLCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113867.1

ORF Insert Sequence:

ATGGACGCGG GAGCCGCCCC GCGGAAGCAC ATGGAGGGAT GCATGAGTGC CATGCAGGCG 
GGGACACAGA TGGTGAAGCT CCGTGGCAGC TCCAAGGGCC TGGTCCGCTT CTACTTCCTG 
GACGAGCATC GCTCCTGCAT CCGCTGGCGG CCCTCACGCA AGAATGAGAA GGCCAAGATT 
TCCATCGACT CCATCCAGGA GGTGAGCGAG GGTCGGCAGT CGGAGATCTT CCAGCGCTAC 
CCCGATGGCA TTTTTGACCC CAACTGCTGC TTCAGCATCT ACCATGGCAG CCACCGAGAG 
TCGCTGGACC TGGTCTCACC CAGCGGGGAC GAGGCGCGCA CCTGGGTCAC CGGCCTGCGC 
TACCTCATGG CAGGCATCCG CGATGAGGAC AGCCTGGCCC GCCGCCAGCG CACTAGGGAC 
CAGTGGCTGA AGCAGACATT TGACGAGGCC GACAAGAACG GGGACGGCAG CCTGAGCATC 
GGTGAGGTCC TGCAGCTGCT GCATAAGCTG AACGTGAACC TGCCCCGGCA GAGGGTGAAG 
CAGATGTTCA AGGAAGCAGA TACAGATGAC CACCAGGGCA CACTGGGCTT TGAGGAGTTC 
TGTGCCTTCT ATAAGATGAT GTCCACCCGC CGGGACCTCT ACCTGCTCAT GCTGACCTAC 
AGTGACCACA AGGACCACCT GAACGCCACT GACCTCCAGC GCTTCCTGGA GGTGGAGCAG 
AAGATGGCGG GTGTGACCCT TGAGAGCTGC CGGGACATCA TCGAGCAGTT TGAGCCCTGC 
CCAGAAAACA AGAGTAAGGG GGTGCTGGGC ATTGATGGCT TCACCAACTA TACTCGGAGT 
CCCGCCGGCG ACATCTTTAA CCCAGAGCAC CATGGCGTGC ACCAGGACAT GACACGGCCG 
CTAAGCCACT ACTTCATCAC CTCATCCCAC AACACCTACC TCGTGGGTGA CCAGCTCATG 
TCCCAGTCTC GGGTAGACAT GTACGCCTGG GTCCTGCAGG CCGGCTGCCG TTGCGTGGAG 
GTGGACTGCT GGGATGGGCC TGACGGGGAG CCCATTGTGC ACCATGGCTA TACTCTGACC 
TCCAAGATCC TCTTCAGAGA TGTCATTGAA ACCATCAACA AATATGCTTT TGTCAAGAAT 
GAGTACCCAG TGATCCTGTC CATTGAGAAC CACTGCAGTG TCATCCAACA GAAGAAGATG 
GCCCAGTATC TAACTGATAT TCTTGGAGAT AAGCTGGACG TGTCATCAGT AAGCAGTGAC 
GATGCCACCT TGCTTCCTTC TCCACAGATG CTCAAGGGAA AGATTCTGGT GAAGGGCAAG 
AAGCTTCCTG CCAACATCAG TGAGGATGCT GAAGAGGGCG AGGTGTCTGA CGAAGACAGT 
GCAGATGAGA TTGACGAGGA CTGCAAACTT CTCAATGGGG ATGTTGCCAC CAACCGGAAG 
CGTGTAGAAA ACATTGCCAA GAAGAAACTG GATTCCCTGA TGAAGGAGTC AAAGATTCGG 
GACTGCGAGG ACCCAAATGA CTTCACTGTG TCCACGCTGC CCCCGTCTGG AAAGCTTGGG 
TACAAGGCAG AAGGCAAAAA GGCTGAGGAG GATGTGGAGT CCGGGGAGGA CGCCGGGGTC 
AGCAGACGGA ACAACCGGAT CCTCATGAGC GGTTTCTCCA AGCGCAAGAA GAAGAGCAGC 
AAGCTAAAGA AGGCGGCCAG CATGGAGGAG GGGGATGAGG ACCTTGACTC CCAGGGCAGT 
CAGAGTCGAG GGGCAAGCCG ACAGAAGAAG ACCATGAAGC TGTCCCGTGC CCTCTCGGAC 
CTGGTGAAGT ACACCAAGTC TGTGGGCACC CACGACGTGG AGGCAGAGGT GGCGTCCAGC 
TGGCAGGTGT CGTCCTTCAG TGAGACCAGG GCCCAGCAGA TCCTGCAGCA GAAGCCGGCA 
CAGTACCTGC GCTTCAACCA GCACCAGCTC TCCCGCATCT ACCCCTCCTC CTACCGCGTG 
GACTCCAGCA ACTACAACCC GCAGCCCTTC TGGAACGCAG GCTGCCAGAT GGTCGCCCTG 
AACTACCAGT CAGAGGGGCG GATGCTGCAG CTGAACCGCG CCAAGTTCAG TGCCAATGGG 
AACTGTGGCT ATGTGCTCAA GCCCCAGTGC ATGTGCCAGG GTGTCTTCAA CCCCAACTCC 
GAGGACCCCC TGCCCGGGCA GCTCAAGAAG CAGCTGGTGC TTCGGATCAT CAGTGGGCAG 
CAGCTCCCCA AGCCAAGGGA CTCGATGCTG GGAGACCGAG GCGAGATCAT CGACCCCTTC 
GTGGAGGTGG AGGTCATCGG GCTTCCCGTG GATTGCAACA GGGAGCAGAC GCGTGTGGTG 
GATGACAATG GATTCAACCC CATGTGGGAA GAGACCCTGG TGTTCACTGT GCACATGCCT 
GAGATCGCAC TGGTGCGCTT CCTCGTCTGG GACCATGACC CCATTGGGCG TGACTTCATT 
GGCCAGAGGA CACTGGCCTT CAGCAGCATG ATGCCAGGCT ATCGGCACGT GTACCTAGAG 
GGGATGGAAG AGGCCTCCAT CTTTGTCCAT GTGGCCGTCA GTGACATCAG TGGTAAGGTC 
AAGCAGGCTC TGGGCCTAAA AGGCCTGTTC CTCAGAGGCA CAAAGCCCGG CTCCCTGGAC 
AGTCATGCTG CTGGGCGGCC CCTGCCCCGG CCTTCCGTTA GCCAGCGGCT CCTGCGGCGC 
ACGGCCAGCG CCCCGACCAA GAGCCAGAAA CCCAGCCGCA AGGCCTTCCC AGAGCTGGTC 
CTGGGCACGC AGGACACGGG CTCCGAGGGG GAGGCCCGTG ACGTGGCCGC CCCCAGCCCT 
GGCCCCGTCC TAGAGGCCTC AGCCCCAGAG GAGCCCGGCA GCCGCAGCCC CCGAGATACC 
CGCCCCTTCT CCATGCAGAG GACGGGCTCC TCTCTATGTG GCCTGGAAAC TATTGCTGAG 
GAGCCAGCCC TGGGCCCCGG CCCCCCACCT CTGGTGGCTG CCCCCCCCAG CCCCTCCCCG 
GGAGGGCCCC TGTGCGCCTC GGGACCTGGC ACCAAAGTGA CGAGCCCCCC AGCGGTCACT 
CTGGGAGCTT CCGTGCCCTT CCAGCCCAGG ACCCGGAGCA GAGGGGACAC TGAGCAGCCC 
CCGGAAAGCC GGCGGCAGGG TTACCATGGT GGGGGGCCTG GAGGTGTGTG TGAGGGGACC 
CCTAGCAGCC AGGTGGTCAG AAGGGCCGAG AGTGAGGGGC AGGTGCCCGC GGAGCACCCG 
GGTGGATGGC GGCCCCCAGC CGGGCCCTGC CCAGCCGTGT ACTCAGACGC CACAGGTGGA 
GACCGGCTGT GGCGGCGGCT GGAACCTGGT GGCCATCGCG ACAGCGTGTC CTCGTCCTCC 
AGTGTGTCAT CCAGCGACAC GGTCATCGAC CTCTCCCTGC CGGGCCTGGG TCTGGGCCTC 
GTCCTGGGCC GTGAGGGTGT CTCGGGGGGT GTCTCGGGGG CTCCAGCTGG CCGCCTGCCC 
CTGCGGCCCT GCCCAGCCAC TGCCGCCCGC CTGGACCTGC CTGCCGTGAC CAAGAGCAAA 
TCCAGCCCCA ACCTGCGGGC CGCCAGCCAG CTGCCCGCTG CACCAGAAGA GCTTCGGCTG 
CGTCCCCTGG TGCCCAGGCT GCCCTGGGGC CACCTTCCCC TGGCAGGCCT CCGGGACTGC 
CCTGCAGCCG CCAAGTCTAA GAGCCTGGGG GACCTGACTG CCGACGACTT TGCCCCCCAG 
GTGGAGATCC TGGGCCGAAG CCTGGGCCTG GCGAGGGAGG GGCGGGCAGG GCGGGGGGCG 
CGGCGGGACG CCCTGACGGA ACAGCTGCGC TGGCTCACAG GCTTCCAGCA GGCCGGTGAC 
ATCACCTCGC CCACCAGCCT GAGTGTCGCT GGGGATGGGG TGCCTGGTCC CCCTGGCTTC 
CTGCGGCGCT CCTCCTCCCG CAGCCAGAGC CGCGTGCGTG CCATTGCCAG CCGGGCCAGG 
CAGGCCCAGG AGCGGCAGCA ACGGCTGCAG AGCCCGAGGG CGCCCCCCGG GGAGGAGCGG 
GGCACCCCCG AGGGTGCCTG CTCAGGGGGC CAAGGGGGCT GCGGGGACGT GCCGGGCATG 
GGCCCCTCCA AGGGCTCCGC TGCCCCCGGC CTCCTGCTCA GACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_014113868.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969343.1 Canis lupus familiaris phospholipase C, eta 2 (PLCH2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe36626
Clone ID Related Accession (Same CDS sequence)	XM_014113868.1
Accession Version	XM_014113868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTGT CCCATCACAA CCTATCAGTG GAGGGATGCA TGAGTGCCAT GCAGGCGGGG 
ACACAGATGG TGAAGCTCCG TGGCAGCTCC AAGGGCCTGG TCCGCTTCTA CTTCCTGGAC 
GAGCATCGCT CCTGCATCCG CTGGCGGCCC TCACGCAAGA ATGAGAAGGC CAAGATTTCC 
ATCGACTCCA TCCAGGAGGT GAGCGAGGGT CGGCAGTCGG AGATCTTCCA GCGCTACCCC 
GATGGCATTT TTGACCCCAA CTGCTGCTTC AGCATCTACC ATGGCAGCCA CCGAGAGTCG 
CTGGACCTGG TCTCACCCAG CGGGGACGAG GCGCGCACCT GGGTCACCGG CCTGCGCTAC 
CTCATGGCAG GCATCCGCGA TGAGGACAGC CTGGCCCGCC GCCAGCGCAC TAGGGACCAG 
TGGCTGAAGC AGACATTTGA CGAGGCCGAC AAGAACGGGG ACGGCAGCCT GAGCATCGGT 
GAGGTCCTGC AGCTGCTGCA TAAGCTGAAC GTGAACCTGC CCCGGCAGAG GGTGAAGCAG 
ATGTTCAAGG AAGCAGATAC AGATGACCAC CAGGGCACAC TGGGCTTTGA GGAGTTCTGT 
GCCTTCTATA AGATGATGTC CACCCGCCGG GACCTCTACC TGCTCATGCT GACCTACAGT 
GACCACAAGG ACCACCTGAA CGCCACTGAC CTCCAGCGCT TCCTGGAGGT GGAGCAGAAG 
ATGGCGGGTG TGACCCTTGA GAGCTGCCGG GACATCATCG AGCAGTTTGA GCCCTGCCCA 
GAAAACAAGA GTAAGGGGGT GCTGGGCATT GATGGCTTCA CCAACTATAC TCGGAGTCCC 
GCCGGCGACA TCTTTAACCC AGAGCACCAT GGCGTGCACC AGGACATGAC ACGGCCGCTA 
AGCCACTACT TCATCACCTC ATCCCACAAC ACCTACCTCG TGGGTGACCA GCTCATGTCC 
CAGTCTCGGG TAGACATGTA CGCCTGGGTC CTGCAGGCCG GCTGCCGTTG CGTGGAGGTG 
GACTGCTGGG ATGGGCCTGA CGGGGAGCCC ATTGTGCACC ATGGCTATAC TCTGACCTCC 
AAGATCCTCT TCAGAGATGT CATTGAAACC ATCAACAAAT ATGCTTTTGT CAAGAATGAG 
TACCCAGTGA TCCTGTCCAT TGAGAACCAC TGCAGTGTCA TCCAACAGAA GAAGATGGCC 
CAGTATCTAA CTGATATTCT TGGAGATAAG CTGGACGTGT CATCAGTAAG CAGTGACGAT 
GCCACCTTGC TTCCTTCTCC ACAGATGCTC AAGGGAAAGA TTCTGGTGAA GGGCAAGAAG 
CTTCCTGCCA ACATCAGTGA GGATGCTGAA GAGGGCGAGG TGTCTGACGA AGACAGTGCA 
GATGAGATTG ACGAGGACTG CAAACTTCTC AATGGGGATG TTGCCACCAA CCGGAAGCGT 
GTAGAAAACA TTGCCAAGAA GAAACTGGAT TCCCTGATGA AGGAGTCAAA GATTCGGGAC 
TGCGAGGACC CAAATGACTT CACTGTGTCC ACGCTGCCCC CGTCTGGAAA GCTTGGGTAC 
AAGGCAGAAG GCAAAAAGGC TGAGGAGGAT GTGGAGTCCG GGGAGGACGC CGGGGTCAGC 
AGACGGAACA ACCGGATCCT CATGAGCGGT TTCTCCAAGC GCAAGAAGAA GAGCAGCAAG 
CTAAAGAAGG CGGCCAGCAT GGAGGAGGGG GATGAGGACC TTGACTCCCA GGGCAGTCAG 
AGTCGAGGGG CAAGCCGACA GAAGAAGACC ATGAAGCTGT CCCGTGCCCT CTCGGACCTG 
GTGAAGTACA CCAAGTCTGT GGGCACCCAC GACGTGGAGG CAGAGGTGGC GTCCAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAGGGCC CAGCAGATCC TGCAGCAGAA GCCGGCACAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCC CGCATCTACC CCTCCTCCTA CCGCGTGGAC 
TCCAGCAACT ACAACCCGCA GCCCTTCTGG AACGCAGGCT GCCAGATGGT CGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTGCAGCTG AACCGCGCCA AGTTCAGTGC CAATGGGAAC 
TGTGGCTATG TGCTCAAGCC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCCGAG 
GACCCCCTGC CCGGGCAGCT CAAGAAGCAG CTGGTGCTTC GGATCATCAG TGGGCAGCAG 
CTCCCCAAGC CAAGGGACTC GATGCTGGGA GACCGAGGCG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATCGGGCT TCCCGTGGAT TGCAACAGGG AGCAGACGCG TGTGGTGGAT 
GACAATGGAT TCAACCCCAT GTGGGAAGAG ACCCTGGTGT TCACTGTGCA CATGCCTGAG 
ATCGCACTGG TGCGCTTCCT CGTCTGGGAC CATGACCCCA TTGGGCGTGA CTTCATTGGC 
CAGAGGACAC TGGCCTTCAG CAGCATGATG CCAGGCTATC GGCACGTGTA CCTAGAGGGG 
ATGGAAGAGG CCTCCATCTT TGTCCATGTG GCCGTCAGTG ACATCAGTGG TAAGGTCAAG 
CAGGCTCTGG GCCTAAAAGG CCTGTTCCTC AGAGGCACAA AGCCCGGCTC CCTGGACAGT 
CATGCTGCTG GGCGGCCCCT GCCCCGGCCT TCCGTTAGCC AGCGGCTCCT GCGGCGCACG 
GCCAGCGCCC CGACCAAGAG CCAGAAACCC AGCCGCAAGG CCTTCCCAGA GCTGGTCCTG 
GGCACGCAGG ACACGGGCTC CGAGGGGGAG GCCCGTGACG TGGCCGCCCC CAGCCCTGGC 
CCCGTCCTAG AGGCCTCAGC CCCAGAGGAG CCCGGCAGCC GCAGCCCCCG AGATACCCGC 
CCCTTCTCCA TGCAGAGGAC GGGCTCCTCT CTATGTGGCC TGGAAACTAT TGCTGAGGAG 
CCAGCCCTGG GCCCCGGCCC CCCACCTCTG GTGGCTGCCC CCCCCAGCCC CTCCCCGGGA 
GGGCCCCTGT GCGCCTCGGG ACCTGGCACC AAAGTGACGA GCCCCCCAGC GGTCACTCTG 
GGAGCTTCCG TGCCCTTCCA GCCCAGGACC CGGAGCAGAG GGGACACTGA GCAGCCCCCG 
GAAAGCCGGC GGCAGGGTTA CCATGGTGGG GGGCCTGGAG GTGTGTGTGA GGGGACCCCT 
AGCAGCCAGG TGGTCAGAAG GGCCGAGAGT GAGGGGCAGG TGCCCGCGGA GCACCCGGGT 
GGATGGCGGC CCCCAGCCGG GCCCTGCCCA GCCGTGTACT CAGACGCCAC AGGTGGAGAC 
CGGCTGTGGC GGCGGCTGGA ACCTGGTGGC CATCGCGACA GCGTGTCCTC GTCCTCCAGT 
GTGTCATCCA GCGACACGGT CATCGACCTC TCCCTGCCGG GCCTGGGTCT GGGCCTCGTC 
CTGGGCCGTG AGGGTGTCTC GGGGGGTGTC TCGGGGGCTC CAGCTGGCCG CCTGCCCCTG 
CGGCCCTGCC CAGCCACTGC CGCCCGCCTG GACCTGCCTG CCGTGACCAA GAGCAAATCC 
AGCCCCAACC TGCGGGCCGC CAGCCAGCTG CCCGCTGCAC CAGAAGAGCT TCGGCTGCGT 
CCCCTGGTGC CCAGGCTGCC CTGGGGCCAC CTTCCCCTGG CAGGCCTCCG GGACTGCCCT 
GCAGCCGCCA AGTCTAAGAG CCTGGGGGAC CTGACTGCCG ACGACTTTGC CCCCCAGGTG 
GAGATCCTGG GCCGAAGCCT GGGCCTGGCG AGGGAGGGGC GGGCAGGGCG GGGGGCGCGG 
CGGGACGCCC TGACGGAACA GCTGCGCTGG CTCACAGGCT TCCAGCAGGC CGGTGACATC 
ACCTCGCCCA CCAGCCTGAG TGTCGCTGGG GATGGGGTGC CTGGTCCCCC TGGCTTCCTG 
CGGCGCTCCT CCTCCCGCAG CCAGAGCCGC GTGCGTGCCA TTGCCAGCCG GGCCAGGCAG 
GCCCAGGAGC GGCAGCAACG GCTGCAGAGC CCGAGGGCGC CCCCCGGGGA GGAGCGGGGC 
ACCCCCGAGG GTGCCTGCTC AGGGGGCCAA GGGGGCTGCG GGGACGTGCC GGGCATGGGC 
CCCTCCAAGG GCTCCGCTGC CCCCGGCCTC CTGCTCAGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969343.1
CDS	190..4314
Translation	MLLSHHNLSVEGCMSAMQAGTQMVKLRGSSKGLVRFYFLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDGIFDPNCCFSIYHGSHRESLDLVSPSGDEARTWVTGLRYLMAGIRDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFKEADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSDHKDHLNATDLQRFLEVEQKMAGVTLESCRDIIEQFEPCPENKSKGVLGIDGFTNYTRSPAGDIFNPEHHGVHQDMTRPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFRDVIETINKYAFVKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDVSSVSSDDATLLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDEDCKLLNGDVATNRKRVENIAKKKLDSLMKESKIRDCEDPNDFTVSTLPPSGKLGYKAEGKKAEEDVESGEDAGVSRRNNRILMSGFSKRKKKSSKLKKAASMEEGDEDLDSQGSQSRGASRQKKTMKLSRALSDLVKYTKSVGTHDVEAEVASSWQVSSFSETRAQQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGNCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCNREQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGTKPGSLDSHAAGRPLPRPSVSQRLLRRTASAPTKSQKPSRKAFPELVLGTQDTGSEGEARDVAAPSPGPVLEASAPEEPGSRSPRDTRPFSMQRTGSSLCGLETIAEEPALGPGPPPLVAAPPSPSPGGPLCASGPGTKVTSPPAVTLGASVPFQPRTRSRGDTEQPPESRRQGYHGGGPGGVCEGTPSSQVVRRAESEGQVPAEHPGGWRPPAGPCPAVYSDATGGDRLWRRLEPGGHRDSVSSSSSVSSSDTVIDLSLPGLGLGLVLGREGVSGGVSGAPAGRLPLRPCPATAARLDLPAVTKSKSSPNLRAASQLPAAPEELRLRPLVPRLPWGHLPLAGLRDCPAAAKSKSLGDLTADDFAPQVEILGRSLGLAREGRAGRGARRDALTEQLRWLTGFQQAGDITSPTSLSVAGDGVPGPPGFLRRSSSRSQSRVRAIASRARQAQERQQRLQSPRAPPGEERGTPEGACSGGQGGCGDVPGMGPSKGSAAPGLLLRL

Target ORF information:

RefSeq Version	XM_014113868.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C, eta 2 (PLCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113868.1

ORF Insert Sequence:

ATGCTTCTGT CCCATCACAA CCTATCAGTG GAGGGATGCA TGAGTGCCAT GCAGGCGGGG 
ACACAGATGG TGAAGCTCCG TGGCAGCTCC AAGGGCCTGG TCCGCTTCTA CTTCCTGGAC 
GAGCATCGCT CCTGCATCCG CTGGCGGCCC TCACGCAAGA ATGAGAAGGC CAAGATTTCC 
ATCGACTCCA TCCAGGAGGT GAGCGAGGGT CGGCAGTCGG AGATCTTCCA GCGCTACCCC 
GATGGCATTT TTGACCCCAA CTGCTGCTTC AGCATCTACC ATGGCAGCCA CCGAGAGTCG 
CTGGACCTGG TCTCACCCAG CGGGGACGAG GCGCGCACCT GGGTCACCGG CCTGCGCTAC 
CTCATGGCAG GCATCCGCGA TGAGGACAGC CTGGCCCGCC GCCAGCGCAC TAGGGACCAG 
TGGCTGAAGC AGACATTTGA CGAGGCCGAC AAGAACGGGG ACGGCAGCCT GAGCATCGGT 
GAGGTCCTGC AGCTGCTGCA TAAGCTGAAC GTGAACCTGC CCCGGCAGAG GGTGAAGCAG 
ATGTTCAAGG AAGCAGATAC AGATGACCAC CAGGGCACAC TGGGCTTTGA GGAGTTCTGT 
GCCTTCTATA AGATGATGTC CACCCGCCGG GACCTCTACC TGCTCATGCT GACCTACAGT 
GACCACAAGG ACCACCTGAA CGCCACTGAC CTCCAGCGCT TCCTGGAGGT GGAGCAGAAG 
ATGGCGGGTG TGACCCTTGA GAGCTGCCGG GACATCATCG AGCAGTTTGA GCCCTGCCCA 
GAAAACAAGA GTAAGGGGGT GCTGGGCATT GATGGCTTCA CCAACTATAC TCGGAGTCCC 
GCCGGCGACA TCTTTAACCC AGAGCACCAT GGCGTGCACC AGGACATGAC ACGGCCGCTA 
AGCCACTACT TCATCACCTC ATCCCACAAC ACCTACCTCG TGGGTGACCA GCTCATGTCC 
CAGTCTCGGG TAGACATGTA CGCCTGGGTC CTGCAGGCCG GCTGCCGTTG CGTGGAGGTG 
GACTGCTGGG ATGGGCCTGA CGGGGAGCCC ATTGTGCACC ATGGCTATAC TCTGACCTCC 
AAGATCCTCT TCAGAGATGT CATTGAAACC ATCAACAAAT ATGCTTTTGT CAAGAATGAG 
TACCCAGTGA TCCTGTCCAT TGAGAACCAC TGCAGTGTCA TCCAACAGAA GAAGATGGCC 
CAGTATCTAA CTGATATTCT TGGAGATAAG CTGGACGTGT CATCAGTAAG CAGTGACGAT 
GCCACCTTGC TTCCTTCTCC ACAGATGCTC AAGGGAAAGA TTCTGGTGAA GGGCAAGAAG 
CTTCCTGCCA ACATCAGTGA GGATGCTGAA GAGGGCGAGG TGTCTGACGA AGACAGTGCA 
GATGAGATTG ACGAGGACTG CAAACTTCTC AATGGGGATG TTGCCACCAA CCGGAAGCGT 
GTAGAAAACA TTGCCAAGAA GAAACTGGAT TCCCTGATGA AGGAGTCAAA GATTCGGGAC 
TGCGAGGACC CAAATGACTT CACTGTGTCC ACGCTGCCCC CGTCTGGAAA GCTTGGGTAC 
AAGGCAGAAG GCAAAAAGGC TGAGGAGGAT GTGGAGTCCG GGGAGGACGC CGGGGTCAGC 
AGACGGAACA ACCGGATCCT CATGAGCGGT TTCTCCAAGC GCAAGAAGAA GAGCAGCAAG 
CTAAAGAAGG CGGCCAGCAT GGAGGAGGGG GATGAGGACC TTGACTCCCA GGGCAGTCAG 
AGTCGAGGGG CAAGCCGACA GAAGAAGACC ATGAAGCTGT CCCGTGCCCT CTCGGACCTG 
GTGAAGTACA CCAAGTCTGT GGGCACCCAC GACGTGGAGG CAGAGGTGGC GTCCAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAGGGCC CAGCAGATCC TGCAGCAGAA GCCGGCACAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCC CGCATCTACC CCTCCTCCTA CCGCGTGGAC 
TCCAGCAACT ACAACCCGCA GCCCTTCTGG AACGCAGGCT GCCAGATGGT CGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTGCAGCTG AACCGCGCCA AGTTCAGTGC CAATGGGAAC 
TGTGGCTATG TGCTCAAGCC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCCGAG 
GACCCCCTGC CCGGGCAGCT CAAGAAGCAG CTGGTGCTTC GGATCATCAG TGGGCAGCAG 
CTCCCCAAGC CAAGGGACTC GATGCTGGGA GACCGAGGCG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATCGGGCT TCCCGTGGAT TGCAACAGGG AGCAGACGCG TGTGGTGGAT 
GACAATGGAT TCAACCCCAT GTGGGAAGAG ACCCTGGTGT TCACTGTGCA CATGCCTGAG 
ATCGCACTGG TGCGCTTCCT CGTCTGGGAC CATGACCCCA TTGGGCGTGA CTTCATTGGC 
CAGAGGACAC TGGCCTTCAG CAGCATGATG CCAGGCTATC GGCACGTGTA CCTAGAGGGG 
ATGGAAGAGG CCTCCATCTT TGTCCATGTG GCCGTCAGTG ACATCAGTGG TAAGGTCAAG 
CAGGCTCTGG GCCTAAAAGG CCTGTTCCTC AGAGGCACAA AGCCCGGCTC CCTGGACAGT 
CATGCTGCTG GGCGGCCCCT GCCCCGGCCT TCCGTTAGCC AGCGGCTCCT GCGGCGCACG 
GCCAGCGCCC CGACCAAGAG CCAGAAACCC AGCCGCAAGG CCTTCCCAGA GCTGGTCCTG 
GGCACGCAGG ACACGGGCTC CGAGGGGGAG GCCCGTGACG TGGCCGCCCC CAGCCCTGGC 
CCCGTCCTAG AGGCCTCAGC CCCAGAGGAG CCCGGCAGCC GCAGCCCCCG AGATACCCGC 
CCCTTCTCCA TGCAGAGGAC GGGCTCCTCT CTATGTGGCC TGGAAACTAT TGCTGAGGAG 
CCAGCCCTGG GCCCCGGCCC CCCACCTCTG GTGGCTGCCC CCCCCAGCCC CTCCCCGGGA 
GGGCCCCTGT GCGCCTCGGG ACCTGGCACC AAAGTGACGA GCCCCCCAGC GGTCACTCTG 
GGAGCTTCCG TGCCCTTCCA GCCCAGGACC CGGAGCAGAG GGGACACTGA GCAGCCCCCG 
GAAAGCCGGC GGCAGGGTTA CCATGGTGGG GGGCCTGGAG GTGTGTGTGA GGGGACCCCT 
AGCAGCCAGG TGGTCAGAAG GGCCGAGAGT GAGGGGCAGG TGCCCGCGGA GCACCCGGGT 
GGATGGCGGC CCCCAGCCGG GCCCTGCCCA GCCGTGTACT CAGACGCCAC AGGTGGAGAC 
CGGCTGTGGC GGCGGCTGGA ACCTGGTGGC CATCGCGACA GCGTGTCCTC GTCCTCCAGT 
GTGTCATCCA GCGACACGGT CATCGACCTC TCCCTGCCGG GCCTGGGTCT GGGCCTCGTC 
CTGGGCCGTG AGGGTGTCTC GGGGGGTGTC TCGGGGGCTC CAGCTGGCCG CCTGCCCCTG 
CGGCCCTGCC CAGCCACTGC CGCCCGCCTG GACCTGCCTG CCGTGACCAA GAGCAAATCC 
AGCCCCAACC TGCGGGCCGC CAGCCAGCTG CCCGCTGCAC CAGAAGAGCT TCGGCTGCGT 
CCCCTGGTGC CCAGGCTGCC CTGGGGCCAC CTTCCCCTGG CAGGCCTCCG GGACTGCCCT 
GCAGCCGCCA AGTCTAAGAG CCTGGGGGAC CTGACTGCCG ACGACTTTGC CCCCCAGGTG 
GAGATCCTGG GCCGAAGCCT GGGCCTGGCG AGGGAGGGGC GGGCAGGGCG GGGGGCGCGG 
CGGGACGCCC TGACGGAACA GCTGCGCTGG CTCACAGGCT TCCAGCAGGC CGGTGACATC 
ACCTCGCCCA CCAGCCTGAG TGTCGCTGGG GATGGGGTGC CTGGTCCCCC TGGCTTCCTG 
CGGCGCTCCT CCTCCCGCAG CCAGAGCCGC GTGCGTGCCA TTGCCAGCCG GGCCAGGCAG 
GCCCAGGAGC GGCAGCAACG GCTGCAGAGC CCGAGGGCGC CCCCCGGGGA GGAGCGGGGC 
ACCCCCGAGG GTGCCTGCTC AGGGGGCCAA GGGGGCTGCG GGGACGTGCC GGGCATGGGC 
CCCTCCAAGG GCTCCGCTGC CCCCGGCCTC CTGCTCAGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PLCH2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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