EPHA1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPHA1
Entrez Gene ID	482735
Full Name	EPH receptor A1
General protein information	Preferred Names EPH receptor A1 Names ephrin type-A receptor 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_539851.4	XP_539851.3	ephrin type-A receptor 1 isoform X1
XM_022403678.1	XP_022259386.1	ephrin type-A receptor 1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	EPHA1	NP_001137567.1
Gallus gallus (chicken)	EPHA1	NP_989691.1
Homo sapiens (human)	EPHA1	NP_005223.4
Mus musculus (house mouse)	Epha1	NP_076069.2
Rattus norvegicus (Norway rat)	Epha1	NP_001101328.1
Macaca mulatta (Rhesus monkey)	EPHA1	XP_001090564.1
Canis lupus familiaris (dog)	EPHA1	XP_539851.3
Pan troglodytes (chimpanzee)	EPHA1	XP_519451.2

Gene Ontology

The following EPHA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPHA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe17942	XM_539851.4 Latest version!	Canis lupus familiaris EPH receptor A1 (EPHA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCe293827	XM_022403678.1 Latest version!	Canis lupus familiaris EPH receptor A1 (EPHA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPHA1 ( XM_539851.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_539851.3 Canis lupus familiaris EPH receptor A1 (EPHA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe17942
Clone ID Related Accession (Same CDS sequence)	XM_539851.4
Accession Version	XM_539851.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	ephrin type-A receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_539851.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC GCTGGCCCCT GGGGCTCGGG CTGCTGCTGC TGCTCCGCGC CCCGCTGCCC 
CCGGGGGCGC GCTCCGAGGA AGTCACTCTG ATGGACACGA GCACAGCACA GGGCGAGCTG 
GGCTGGCTGC TGGATCCCCC GGAGGATGGG TGGAGTGAGG TGCAGCAGAT CCTGAACGGG 
ACGCCCCTTT ACATGTACCA GGACTGCCCA GTGCAAGCAG GCGGGGACAC TGACCACTGG 
CTGCGCTCCA ACTGGATCTA CCGCGGGGAG GAAGCTTCTC GGGTCCACGT GGAGCTGCAG 
TTCACCGTGA GGGACTGTAA GAGTTTCCCT GGCGGAGCCG GGCCTCTGGG CTGCAAGGAG 
ACCTTCAACC TCCTGTACAT GGAGAGTGAC CAGGACGTGG GCATCCAGCT CCGACGGCCC 
TTGTTCCAAA AGGTAACTAC TGTGGCTGCA GACCAGAGCT TCACCATCCG GGACCTCGCG 
TCTGGCGCTG TGAAACTGAA CGTGGAGCGC TGCTCCTTGG GGCACCTGAC CCGCCGAGGC 
CTCTACCTCG CTTTCCACAA CCCGGGTGCC TGTGTGGCCC TGGTGTCCGT AAGGGTCTTT 
TACCAGCGCT GTCCTGAGGT CGTGCAGGGC TTGGCCCGAT TCCCCAACAC TCTGCCCGGA 
CCTGGCGGGC TGGTGGAGGT GGCAGGGACC TGCTTGCCCC ACGCCCAGGT CAGCCCCGGC 
CCCTCAGGTG CGCCCCGCAT GCACTGCAGC CCCGACGGGG AGTGGCTGGT GCCTGTGGGA 
CGGTGCCACT GTGAGCCCGG CTATGAAGAA GGCGGCAGTG GTGAAGGATG CCTGGCCTGT 
CCCAGCGGCT CCTACCGGGC GGACGCAGAT GCGCCCCATT GTGTCAAGTG CCCAGGACAC 
AGCGCAGCTG AGTCCGAGGG GGCCACCCTC TGCATCTGCC ACAGCGGCCA TTTCCGAGCC 
CCTGGGGAGG GCCCCCAGGT GTCCTGCACA CGACCCCCCT CAGTTCCCCG AAACCTGAGC 
TTCTCTGTGT TGGGGACTCA GCTCTCGCTG CGTTGGGAAC CCCCGGCGGA TATGGGGGGG 
CGCCAGGATG TGAGGTACAG CGTGGGGTGT TCACAGTGCC GGGGAGCAGC GGTGGACGGG 
GGGCCCTGCC AGCCCTGCGG GGGAAGTGTG CGCTTTTCCC CAGGAGCCAG CGGGCTCACG 
GCGCCAGCCG TGCGCGTCAA CGGCCTTGAA CCCTACGCCA ACTACACCTT TAACGTGGAG 
GCCCAGAACG GAGTGTCGGG GCTGGGCACC TCCACCCCCG CCAGCGCCTC CCTGAGCGTC 
AGCATGGGCC ACGCAGAGTC CCTGTCAGGC CTGTCCCTGA GGCTGGTGAA GAAGGAGCCG 
CGGCAGCTGG AACTGACCTG GGCAGGATCC AGGCCGCGCA GCCCCGGGGC GAACCTGAGC 
TACGAGCTGC ACGTGCTGAA TCAGGACGAA GAACGGCATC AGATGGTTCT GGAACCCAGG 
GTCTTGCTGA CTGAGCTGCA GCCAGACACC ACGTACATTG TCAGGGTTCG AATGTTAACC 
CCTCTAGGTC CTGGCCCGTT CTCCCCTGAC CATGAGTTTC GGACAAGCCC ACCAGTTTCC 
AGGGGCCTGA CCGGAGGAGA GATTGTGGCC ATCATCTTCG GGCTGCTGCT GGGTGTCGCT 
CTGCTGATGG GGATGCTCTT CTTCCGATCA AGGAAAGCGC AGCGGAGACA CCCGCAGAGG 
CAACGTGACC GTGTCGCCGA AGTGAACCGA GAGGACAAGC TGTGGCTGAA GCCTTATGTG 
GACCTCCAAG CGTATGAGGA CCCTGCCCAG GGAGCCCTGG ACTTCACCCA GGAGCTTGAT 
CCAGGCTGGC TGATTGTGGA CACTGTCATA GGGGAAGGAG AGTTTGGAGA AGTCTATCGA 
GGGACCCTGA AAATCCCTAG CCAGGACTGT AAGACTGTGG CCATTAAGAC CTTGAAAGAC 
ACGTCTCCAG ACGGCCAGTG GTGGAACTTC CTTCGAGAGG CAACTATCAT GGGCCAGTTC 
AACCACCCGC ACATTTTGCA CCTGGAAGGC GTTGTCACAA AGCGAAAGCC CATCATGATC 
ATCACGGAGT TTATGGAGAA TGGAGCCCTG GATGCCTTCC TGAGGGAGCG GGAGGACCAG 
CTGGTCCCTG GGCAGCTGGT GGCCATGCTG CAGGGCATTG CATCTGGCAT GAACTACCTC 
AGTGACCACA ATTACGTCCA CCGGGACCTC GCGGCCAGGA ACATCTTAGT GAGTCAGAAC 
CTGTGCTGCA AGGTGTCTGA CTTCGGCCTG ACCCGCCTCC TGGACAACTT CGATGGCACC 
TATGAAACCC AGGGGGGAAA GATCCCCATC CGTTGGACAG CCCCTGAAGC CATTGCCCAC 
CGCACCTTCA CCACAGCCAG CGATGTGTGG AGCTTTGGGA TTGTGATGTG GGAGGTGCTG 
AGTTTTGGAG ACAAGCCCTA TGGGGAGATG AGCAATCAGG AGGTAATGAA GAGCATTGAG 
GATGGGTACC GGCTGCCGCC TCCCGTGGAC TGCCCTGCCC CTCTCTATGA ACTCATGAAG 
AACTGCTGGG CCTATGACCG GGCCCGTCGG CCCTCCTTCC ACCAGCTGAA GGCACATCTG 
GACCAGTTGC TTGCCAACCC CCACTCACTG CGGACCATTG CTAACTTTGA TCCCAGGGTG 
ACGCTTCGCC TGCCCAGCCT CAGTGGCTCC GACGGGATCC CCTATCGAAG CGTCCCCGAG 
TGGCTGGAGT CTATACGCAT GAAACGCTAC ATTCTGCACT TCCGCTCAGC GGGGCTGGAC 
ACCATGGAGT GTGTGCTCGA GCTGACGGCC GAGGACCTGG CCCAGATGGG GATCACGCTG 
CCAGGACACC AGAAGCGCAT TCTTTGCAGT ATTCAGGGAT TCAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539851.3
CDS	62..2992
Translation	MERRWPLGLGLLLLLRAPLPPGARSEEVTLMDTSTAQGELGWLLDPPEDGWSEVQQILNGTPLYMYQDCPVQAGGDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGAVKLNVERCSLGHLTRRGLYLAFHNPGACVALVSVRVFYQRCPEVVQGLARFPNTLPGPGGLVEVAGTCLPHAQVSPGPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEGCLACPSGSYRADADAPHCVKCPGHSAAESEGATLCICHSGHFRAPGEGPQVSCTRPPSVPRNLSFSVLGTQLSLRWEPPADMGGRQDVRYSVGCSQCRGAAVDGGPCQPCGGSVRFSPGASGLTAPAVRVNGLEPYANYTFNVEAQNGVSGLGTSTPASASLSVSMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLSYELHVLNQDEERHQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGEIVAIIFGLLLGVALLMGMLFFRSRKAQRRHPQRQRDRVAEVNREDKLWLKPYVDLQAYEDPAQGALDFTQELDPGWLIVDTVIGEGEFGEVYRGTLKIPSQDCKTVAIKTLKDTSPDGQWWNFLREATIMGQFNHPHILHLEGVVTKRKPIMIITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSDHNYVHRDLAARNILVSQNLCCKVSDFGLTRLLDNFDGTYETQGGKIPIRWTAPEAIAHRTFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIEDGYRLPPPVDCPAPLYELMKNCWAYDRARRPSFHQLKAHLDQLLANPHSLRTIANFDPRVTLRLPSLSGSDGIPYRSVPEWLESIRMKRYILHFRSAGLDTMECVLELTAEDLAQMGITLPGHQKRILCSIQGFKD

Target ORF information:

RefSeq Version	XM_539851.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EPH receptor A1 (EPHA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539851.4

ORF Insert Sequence:

ATGGAGCGGC GCTGGCCCCT GGGGCTCGGG CTGCTGCTGC TGCTCCGCGC CCCGCTGCCC 
CCGGGGGCGC GCTCCGAGGA AGTCACTCTG ATGGACACGA GCACAGCACA GGGCGAGCTG 
GGCTGGCTGC TGGATCCCCC GGAGGATGGG TGGAGTGAGG TGCAGCAGAT CCTGAACGGG 
ACGCCCCTTT ACATGTACCA GGACTGCCCA GTGCAAGCAG GCGGGGACAC TGACCACTGG 
CTGCGCTCCA ACTGGATCTA CCGCGGGGAG GAAGCTTCTC GGGTCCACGT GGAGCTGCAG 
TTCACCGTGA GGGACTGTAA GAGTTTCCCT GGCGGAGCCG GGCCTCTGGG CTGCAAGGAG 
ACCTTCAACC TCCTGTACAT GGAGAGTGAC CAGGACGTGG GCATCCAGCT CCGACGGCCC 
TTGTTCCAAA AGGTAACTAC TGTGGCTGCA GACCAGAGCT TCACCATCCG GGACCTCGCG 
TCTGGCGCTG TGAAACTGAA CGTGGAGCGC TGCTCCTTGG GGCACCTGAC CCGCCGAGGC 
CTCTACCTCG CTTTCCACAA CCCGGGTGCC TGTGTGGCCC TGGTGTCCGT AAGGGTCTTT 
TACCAGCGCT GTCCTGAGGT CGTGCAGGGC TTGGCCCGAT TCCCCAACAC TCTGCCCGGA 
CCTGGCGGGC TGGTGGAGGT GGCAGGGACC TGCTTGCCCC ACGCCCAGGT CAGCCCCGGC 
CCCTCAGGTG CGCCCCGCAT GCACTGCAGC CCCGACGGGG AGTGGCTGGT GCCTGTGGGA 
CGGTGCCACT GTGAGCCCGG CTATGAAGAA GGCGGCAGTG GTGAAGGATG CCTGGCCTGT 
CCCAGCGGCT CCTACCGGGC GGACGCAGAT GCGCCCCATT GTGTCAAGTG CCCAGGACAC 
AGCGCAGCTG AGTCCGAGGG GGCCACCCTC TGCATCTGCC ACAGCGGCCA TTTCCGAGCC 
CCTGGGGAGG GCCCCCAGGT GTCCTGCACA CGACCCCCCT CAGTTCCCCG AAACCTGAGC 
TTCTCTGTGT TGGGGACTCA GCTCTCGCTG CGTTGGGAAC CCCCGGCGGA TATGGGGGGG 
CGCCAGGATG TGAGGTACAG CGTGGGGTGT TCACAGTGCC GGGGAGCAGC GGTGGACGGG 
GGGCCCTGCC AGCCCTGCGG GGGAAGTGTG CGCTTTTCCC CAGGAGCCAG CGGGCTCACG 
GCGCCAGCCG TGCGCGTCAA CGGCCTTGAA CCCTACGCCA ACTACACCTT TAACGTGGAG 
GCCCAGAACG GAGTGTCGGG GCTGGGCACC TCCACCCCCG CCAGCGCCTC CCTGAGCGTC 
AGCATGGGCC ACGCAGAGTC CCTGTCAGGC CTGTCCCTGA GGCTGGTGAA GAAGGAGCCG 
CGGCAGCTGG AACTGACCTG GGCAGGATCC AGGCCGCGCA GCCCCGGGGC GAACCTGAGC 
TACGAGCTGC ACGTGCTGAA TCAGGACGAA GAACGGCATC AGATGGTTCT GGAACCCAGG 
GTCTTGCTGA CTGAGCTGCA GCCAGACACC ACGTACATTG TCAGGGTTCG AATGTTAACC 
CCTCTAGGTC CTGGCCCGTT CTCCCCTGAC CATGAGTTTC GGACAAGCCC ACCAGTTTCC 
AGGGGCCTGA CCGGAGGAGA GATTGTGGCC ATCATCTTCG GGCTGCTGCT GGGTGTCGCT 
CTGCTGATGG GGATGCTCTT CTTCCGATCA AGGAAAGCGC AGCGGAGACA CCCGCAGAGG 
CAACGTGACC GTGTCGCCGA AGTGAACCGA GAGGACAAGC TGTGGCTGAA GCCTTATGTG 
GACCTCCAAG CGTATGAGGA CCCTGCCCAG GGAGCCCTGG ACTTCACCCA GGAGCTTGAT 
CCAGGCTGGC TGATTGTGGA CACTGTCATA GGGGAAGGAG AGTTTGGAGA AGTCTATCGA 
GGGACCCTGA AAATCCCTAG CCAGGACTGT AAGACTGTGG CCATTAAGAC CTTGAAAGAC 
ACGTCTCCAG ACGGCCAGTG GTGGAACTTC CTTCGAGAGG CAACTATCAT GGGCCAGTTC 
AACCACCCGC ACATTTTGCA CCTGGAAGGC GTTGTCACAA AGCGAAAGCC CATCATGATC 
ATCACGGAGT TTATGGAGAA TGGAGCCCTG GATGCCTTCC TGAGGGAGCG GGAGGACCAG 
CTGGTCCCTG GGCAGCTGGT GGCCATGCTG CAGGGCATTG CATCTGGCAT GAACTACCTC 
AGTGACCACA ATTACGTCCA CCGGGACCTC GCGGCCAGGA ACATCTTAGT GAGTCAGAAC 
CTGTGCTGCA AGGTGTCTGA CTTCGGCCTG ACCCGCCTCC TGGACAACTT CGATGGCACC 
TATGAAACCC AGGGGGGAAA GATCCCCATC CGTTGGACAG CCCCTGAAGC CATTGCCCAC 
CGCACCTTCA CCACAGCCAG CGATGTGTGG AGCTTTGGGA TTGTGATGTG GGAGGTGCTG 
AGTTTTGGAG ACAAGCCCTA TGGGGAGATG AGCAATCAGG AGGTAATGAA GAGCATTGAG 
GATGGGTACC GGCTGCCGCC TCCCGTGGAC TGCCCTGCCC CTCTCTATGA ACTCATGAAG 
AACTGCTGGG CCTATGACCG GGCCCGTCGG CCCTCCTTCC ACCAGCTGAA GGCACATCTG 
GACCAGTTGC TTGCCAACCC CCACTCACTG CGGACCATTG CTAACTTTGA TCCCAGGGTG 
ACGCTTCGCC TGCCCAGCCT CAGTGGCTCC GACGGGATCC CCTATCGAAG CGTCCCCGAG 
TGGCTGGAGT CTATACGCAT GAAACGCTAC ATTCTGCACT TCCGCTCAGC GGGGCTGGAC 
ACCATGGAGT GTGTGCTCGA GCTGACGGCC GAGGACCTGG CCCAGATGGG GATCACGCTG 
CCAGGACACC AGAAGCGCAT TCTTTGCAGT ATTCAGGGAT TCAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPHA1 ( XM_022403678.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259386.1 Canis lupus familiaris EPH receptor A1 (EPHA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe293827
Clone ID Related Accession (Same CDS sequence)	XM_022403678.1
Accession Version	XM_022403678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	ephrin type-A receptor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA GCACAGCACA GGGCGAGCTG GGCTGGCTGC TGGATCCCCC GGAGGATGGG 
TGGAGTGAGG TGCAGCAGAT CCTGAACGGG ACGCCCCTTT ACATGTACCA GGACTGCCCA 
GTGCAAGCAG GCGGGGACAC TGACCACTGG CTGCGCTCCA ACTGGATCTA CCGCGGGGAG 
GAAGCTTCTC GGGTCCACGT GGAGCTGCAG TTCACCGTGA GGGACTGTAA GAGTTTCCCT 
GGCGGAGCCG GGCCTCTGGG CTGCAAGGAG ACCTTCAACC TCCTGTACAT GGAGAGTGAC 
CAGGACGTGG GCATCCAGCT CCGACGGCCC TTGTTCCAAA AGGTAACTAC TGTGGCTGCA 
GACCAGAGCT TCACCATCCG GGACCTCGCG TCTGGCGCTG TGAAACTGAA CGTGGAGCGC 
TGCTCCTTGG GGCACCTGAC CCGCCGAGGC CTCTACCTCG CTTTCCACAA CCCGGGTGCC 
TGTGTGGCCC TGGTGTCCGT AAGGGTCTTT TACCAGCGCT GTCCTGAGGT CGTGCAGGGC 
TTGGCCCGAT TCCCCAACAC TCTGCCCGGA CCTGGCGGGC TGGTGGAGGT GGCAGGGACC 
TGCTTGCCCC ACGCCCAGGT CAGCCCCGGC CCCTCAGGTG CGCCCCGCAT GCACTGCAGC 
CCCGACGGGG AGTGGCTGGT GCCTGTGGGA CGGTGCCACT GTGAGCCCGG CTATGAAGAA 
GGCGGCAGTG GTGAAGGATG CCTGGCCTGT CCCAGCGGCT CCTACCGGGC GGACGCAGAT 
GCGCCCCATT GTGTCAAGTG CCCAGGACAC AGCGCAGCTG AGTCCGAGGG GGCCACCCTC 
TGCATCTGCC ACAGCGGCCA TTTCCGAGCC CCTGGGGAGG GCCCCCAGGT GTCCTGCACA 
CGACCCCCCT CAGTTCCCCG AAACCTGAGC TTCTCTGTGT TGGGGACTCA GCTCTCGCTG 
CGTTGGGAAC CCCCGGCGGA TATGGGGGGG CGCCAGGATG TGAGGTACAG CGTGGGGTGT 
TCACAGTGCC GGGGAGCAGC GGTGGACGGG GGGCCCTGCC AGCCCTGCGG GGGAAGTGTG 
CGCTTTTCCC CAGGAGCCAG CGGGCTCACG GCGCCAGCCG TGCGCGTCAA CGGCCTTGAA 
CCCTACGCCA ACTACACCTT TAACGTGGAG GCCCAGAACG GAGTGTCGGG GCTGGGCACC 
TCCACCCCCG CCAGCGCCTC CCTGAGCGTC AGCATGGGCC ACGCAGAGTC CCTGTCAGGC 
CTGTCCCTGA GGCTGGTGAA GAAGGAGCCG CGGCAGCTGG AACTGACCTG GGCAGGATCC 
AGGCCGCGCA GCCCCGGGGC GAACCTGAGC TACGAGCTGC ACGTGCTGAA TCAGGACGAA 
GAACGGCATC AGATGGTTCT GGAACCCAGG GTCTTGCTGA CTGAGCTGCA GCCAGACACC 
ACGTACATTG TCAGGGTTCG AATGTTAACC CCTCTAGGTC CTGGCCCGTT CTCCCCTGAC 
CATGAGTTTC GGACAAGCCC ACCAGTTTCC AGGGGCCTGA CCGGAGGAGA GATTGTGGCC 
ATCATCTTCG GGCTGCTGCT GGGTGTCGCT CTGCTGATGG GGATGCTCTT CTTCCGATCA 
AGGAAAGCGC AGCGGAGACA CCCGCAGAGG CAACGTGACC GTGTCGCCGA AGTGAACCGA 
GAGGACAAGC TGTGGCTGAA GCCTTATGTG GACCTCCAAG CGTATGAGGA CCCTGCCCAG 
GGAGCCCTGG ACTTCACCCA GGAGCTTGAT CCAGGCTGGC TGATTGTGGA CACTGTCATA 
GGGGAAGGAG AGTTTGGAGA AGTCTATCGA GGGACCCTGA AAATCCCTAG CCAGGACTGT 
AAGACTGTGG CCATTAAGAC CTTGAAAGAC ACGTCTCCAG ACGGCCAGTG GTGGAACTTC 
CTTCGAGAGG CAACTATCAT GGGCCAGTTC AACCACCCGC ACATTTTGCA CCTGGAAGGC 
GTTGTCACAA AGCGAAAGCC CATCATGATC ATCACGGAGT TTATGGAGAA TGGAGCCCTG 
GATGCCTTCC TGAGGGAGCG GGAGGACCAG CTGGTCCCTG GGCAGCTGGT GGCCATGCTG 
CAGGGCATTG CATCTGGCAT GAACTACCTC AGTGACCACA ATTACGTCCA CCGGGACCTC 
GCGGCCAGGA ACATCTTAGT GAGTCAGAAC CTGTGCTGCA AGGTGTCTGA CTTCGGCCTG 
ACCCGCCTCC TGGACAACTT CGATGGCACC TATGAAACCC AGGGGGGAAA GATCCCCATC 
CGTTGGACAG CCCCTGAAGC CATTGCCCAC CGCACCTTCA CCACAGCCAG CGATGTGTGG 
AGCTTTGGGA TTGTGATGTG GGAGGTGCTG AGTTTTGGAG ACAAGCCCTA TGGGGAGATG 
AGCAATCAGG AGGTAATGAA GAGCATTGAG GATGGGTACC GGCTGCCGCC TCCCGTGGAC 
TGCCCTGCCC CTCTCTATGA ACTCATGAAG AACTGCTGGG CCTATGACCG GGCCCGTCGG 
CCCTCCTTCC ACCAGCTGAA GGCACATCTG GACCAGTTGC TTGCCAACCC CCACTCACTG 
CGGACCATTG CTAACTTTGA TCCCAGGGTG ACGCTTCGCC TGCCCAGCCT CAGTGGCTCC 
GACGGGATCC CCTATCGAAG CGTCCCCGAG TGGCTGGAGT CTATACGCAT GAAACGCTAC 
ATTCTGCACT TCCGCTCAGC GGGGCTGGAC ACCATGGAGT GTGTGCTCGA GCTGACGGCC 
GAGGACCTGG CCCAGATGGG GATCACGCTG CCAGGACACC AGAAGCGCAT TCTTTGCAGT 
ATTCAGGGAT TCAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259386.1
CDS	29..2869
Translation	MDTSTAQGELGWLLDPPEDGWSEVQQILNGTPLYMYQDCPVQAGGDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGAVKLNVERCSLGHLTRRGLYLAFHNPGACVALVSVRVFYQRCPEVVQGLARFPNTLPGPGGLVEVAGTCLPHAQVSPGPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEGCLACPSGSYRADADAPHCVKCPGHSAAESEGATLCICHSGHFRAPGEGPQVSCTRPPSVPRNLSFSVLGTQLSLRWEPPADMGGRQDVRYSVGCSQCRGAAVDGGPCQPCGGSVRFSPGASGLTAPAVRVNGLEPYANYTFNVEAQNGVSGLGTSTPASASLSVSMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLSYELHVLNQDEERHQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGEIVAIIFGLLLGVALLMGMLFFRSRKAQRRHPQRQRDRVAEVNREDKLWLKPYVDLQAYEDPAQGALDFTQELDPGWLIVDTVIGEGEFGEVYRGTLKIPSQDCKTVAIKTLKDTSPDGQWWNFLREATIMGQFNHPHILHLEGVVTKRKPIMIITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSDHNYVHRDLAARNILVSQNLCCKVSDFGLTRLLDNFDGTYETQGGKIPIRWTAPEAIAHRTFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIEDGYRLPPPVDCPAPLYELMKNCWAYDRARRPSFHQLKAHLDQLLANPHSLRTIANFDPRVTLRLPSLSGSDGIPYRSVPEWLESIRMKRYILHFRSAGLDTMECVLELTAEDLAQMGITLPGHQKRILCSIQGFKD

Target ORF information:

RefSeq Version	XM_022403678.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EPH receptor A1 (EPHA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403678.1

ORF Insert Sequence:

ATGGACACGA GCACAGCACA GGGCGAGCTG GGCTGGCTGC TGGATCCCCC GGAGGATGGG 
TGGAGTGAGG TGCAGCAGAT CCTGAACGGG ACGCCCCTTT ACATGTACCA GGACTGCCCA 
GTGCAAGCAG GCGGGGACAC TGACCACTGG CTGCGCTCCA ACTGGATCTA CCGCGGGGAG 
GAAGCTTCTC GGGTCCACGT GGAGCTGCAG TTCACCGTGA GGGACTGTAA GAGTTTCCCT 
GGCGGAGCCG GGCCTCTGGG CTGCAAGGAG ACCTTCAACC TCCTGTACAT GGAGAGTGAC 
CAGGACGTGG GCATCCAGCT CCGACGGCCC TTGTTCCAAA AGGTAACTAC TGTGGCTGCA 
GACCAGAGCT TCACCATCCG GGACCTCGCG TCTGGCGCTG TGAAACTGAA CGTGGAGCGC 
TGCTCCTTGG GGCACCTGAC CCGCCGAGGC CTCTACCTCG CTTTCCACAA CCCGGGTGCC 
TGTGTGGCCC TGGTGTCCGT AAGGGTCTTT TACCAGCGCT GTCCTGAGGT CGTGCAGGGC 
TTGGCCCGAT TCCCCAACAC TCTGCCCGGA CCTGGCGGGC TGGTGGAGGT GGCAGGGACC 
TGCTTGCCCC ACGCCCAGGT CAGCCCCGGC CCCTCAGGTG CGCCCCGCAT GCACTGCAGC 
CCCGACGGGG AGTGGCTGGT GCCTGTGGGA CGGTGCCACT GTGAGCCCGG CTATGAAGAA 
GGCGGCAGTG GTGAAGGATG CCTGGCCTGT CCCAGCGGCT CCTACCGGGC GGACGCAGAT 
GCGCCCCATT GTGTCAAGTG CCCAGGACAC AGCGCAGCTG AGTCCGAGGG GGCCACCCTC 
TGCATCTGCC ACAGCGGCCA TTTCCGAGCC CCTGGGGAGG GCCCCCAGGT GTCCTGCACA 
CGACCCCCCT CAGTTCCCCG AAACCTGAGC TTCTCTGTGT TGGGGACTCA GCTCTCGCTG 
CGTTGGGAAC CCCCGGCGGA TATGGGGGGG CGCCAGGATG TGAGGTACAG CGTGGGGTGT 
TCACAGTGCC GGGGAGCAGC GGTGGACGGG GGGCCCTGCC AGCCCTGCGG GGGAAGTGTG 
CGCTTTTCCC CAGGAGCCAG CGGGCTCACG GCGCCAGCCG TGCGCGTCAA CGGCCTTGAA 
CCCTACGCCA ACTACACCTT TAACGTGGAG GCCCAGAACG GAGTGTCGGG GCTGGGCACC 
TCCACCCCCG CCAGCGCCTC CCTGAGCGTC AGCATGGGCC ACGCAGAGTC CCTGTCAGGC 
CTGTCCCTGA GGCTGGTGAA GAAGGAGCCG CGGCAGCTGG AACTGACCTG GGCAGGATCC 
AGGCCGCGCA GCCCCGGGGC GAACCTGAGC TACGAGCTGC ACGTGCTGAA TCAGGACGAA 
GAACGGCATC AGATGGTTCT GGAACCCAGG GTCTTGCTGA CTGAGCTGCA GCCAGACACC 
ACGTACATTG TCAGGGTTCG AATGTTAACC CCTCTAGGTC CTGGCCCGTT CTCCCCTGAC 
CATGAGTTTC GGACAAGCCC ACCAGTTTCC AGGGGCCTGA CCGGAGGAGA GATTGTGGCC 
ATCATCTTCG GGCTGCTGCT GGGTGTCGCT CTGCTGATGG GGATGCTCTT CTTCCGATCA 
AGGAAAGCGC AGCGGAGACA CCCGCAGAGG CAACGTGACC GTGTCGCCGA AGTGAACCGA 
GAGGACAAGC TGTGGCTGAA GCCTTATGTG GACCTCCAAG CGTATGAGGA CCCTGCCCAG 
GGAGCCCTGG ACTTCACCCA GGAGCTTGAT CCAGGCTGGC TGATTGTGGA CACTGTCATA 
GGGGAAGGAG AGTTTGGAGA AGTCTATCGA GGGACCCTGA AAATCCCTAG CCAGGACTGT 
AAGACTGTGG CCATTAAGAC CTTGAAAGAC ACGTCTCCAG ACGGCCAGTG GTGGAACTTC 
CTTCGAGAGG CAACTATCAT GGGCCAGTTC AACCACCCGC ACATTTTGCA CCTGGAAGGC 
GTTGTCACAA AGCGAAAGCC CATCATGATC ATCACGGAGT TTATGGAGAA TGGAGCCCTG 
GATGCCTTCC TGAGGGAGCG GGAGGACCAG CTGGTCCCTG GGCAGCTGGT GGCCATGCTG 
CAGGGCATTG CATCTGGCAT GAACTACCTC AGTGACCACA ATTACGTCCA CCGGGACCTC 
GCGGCCAGGA ACATCTTAGT GAGTCAGAAC CTGTGCTGCA AGGTGTCTGA CTTCGGCCTG 
ACCCGCCTCC TGGACAACTT CGATGGCACC TATGAAACCC AGGGGGGAAA GATCCCCATC 
CGTTGGACAG CCCCTGAAGC CATTGCCCAC CGCACCTTCA CCACAGCCAG CGATGTGTGG 
AGCTTTGGGA TTGTGATGTG GGAGGTGCTG AGTTTTGGAG ACAAGCCCTA TGGGGAGATG 
AGCAATCAGG AGGTAATGAA GAGCATTGAG GATGGGTACC GGCTGCCGCC TCCCGTGGAC 
TGCCCTGCCC CTCTCTATGA ACTCATGAAG AACTGCTGGG CCTATGACCG GGCCCGTCGG 
CCCTCCTTCC ACCAGCTGAA GGCACATCTG GACCAGTTGC TTGCCAACCC CCACTCACTG 
CGGACCATTG CTAACTTTGA TCCCAGGGTG ACGCTTCGCC TGCCCAGCCT CAGTGGCTCC 
GACGGGATCC CCTATCGAAG CGTCCCCGAG TGGCTGGAGT CTATACGCAT GAAACGCTAC 
ATTCTGCACT TCCGCTCAGC GGGGCTGGAC ACCATGGAGT GTGTGCTCGA GCTGACGGCC 
GAGGACCTGG CCCAGATGGG GATCACGCTG CCAGGACACC AGAAGCGCAT TCTTTGCAGT 
ATTCAGGGAT TCAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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EPHA1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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