PDE6A cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE6A
Entrez Gene ID	403620
Full Name	phosphodiesterase 6A
Synonyms	PDEA
General protein information	Preferred Names phosphodiesterase 6A Names rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha GMP-PDE alpha cGMP-phosphodiesterase alpha-subunit rod specific cGMP phosphodiesterase, alpha subunit
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003073.1	NP_001003073.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
XM_005618863.2	XP_005618920.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE6A	NP_000431.2
Pan troglodytes (chimpanzee)	PDE6A	XP_518030.2
Mus musculus (house mouse)	Pde6a	NP_666198.1
Rattus norvegicus (Norway rat)	Pde6a	NP_001100856.1
Xenopus tropicalis (tropical clawed frog)	pde6a	XP_002936750.2
Canis lupus familiaris (dog)	PDE6A	NP_001003073.1
Danio rerio (zebrafish)	pde6a	NP_001007161.1
Bos taurus (cattle)	PDE6A	NP_001001526.2
Macaca mulatta (Rhesus monkey)	PDE6A	XP_001107349.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of a canine model of autosomal recessive retinitis pigmentosa due to a PDE6A mutation.
Tuntivanich N, Pittler SJ, Fischer AJ, Omar G, Kiupel M, Weber A, Yao S, Steibel JP, Khan NW, Petersen-Jones SM
Investigative ophthalmology & visual science50(2)801-13(2009 Feb)

Exclusion of the PDE6A gene for generalised progressive retinal atrophy in 11 breeds of dog.
Dekomien G, Epplen JT
Animal genetics31(2)135-9(2000 Apr)

cGMP phosphodiesterase-alpha mutation causes progressive retinal atrophy in the Cardigan Welsh corgi dog.
Petersen-Jones SM, Entz DD, Sargan DR
Investigative ophthalmology & visual science40(8)1637-44(1999 Jul)

Microsatellite (CA)n polymorphism in intron 2 of the canine rod photoreceptor cGMP-phosphodiesterase alpha-subunit gene.
Veske A, Nilsson SE, Gal A
Animal genetics28(5)380(1997 Oct)

Cloning of the cDNA encoding rod photoreceptor cGMP-phosphodiesterase alpha and gamma subunits from the retinal degenerate Labrador retriever dog.
Kylmä T, Paulin L, Hurwitz MY, Hurwitz RL, Kommonen B
Research in veterinary science62(3)293-6(1997 May-Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDE6A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE6A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe33655	NM_001003073.1 Latest version!	Canis lupus familiaris phosphodiesterase 6A (PDE6A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCe04855	XM_005618863.2 Latest version!	Canis lupus familiaris phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE6A ( NM_001003073.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> NP_001003073.1 Canis lupus familiaris phosphodiesterase 6A (PDE6A), mRNA.

Price & Availability↑

CloneID	OCe33655
Clone ID Related Accession (Same CDS sequence)	NM_001003073.1
Accession Version	NM_001003073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1470585600000
Organism	Canis lupus familiaris(dog)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U52868.1. Sequence Note: This sequence has been modified as follows: removed 34 bp suspected to be vector contamination from the 5' end. ##Evidence-Data-START## Transcript exon combination :: U52868.1, Y13282.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00013599 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGAGG TGACAGCAGA GCAGGTGGAG AAGTTCCTGG ACTCGAATAT TATCTTTGCC 
AAACAGTACT ACAACCTCCG CTACCGGGCC AAGGTCATCT CAGACATGCT GGGGGCCAAG 
GAGGCAGCGG TGGACTTCAG CAACTACCAC TCGCTGAGCA GTGTGGAGGA GAGTGAAATC 
ATCTTTGACC TCCTGCGAGA CTTCCAGGAG AATTTGCAGG CCGAGAGATG CATCTTCAAT 
GTCATGAAGA AGCTGTGCTT CCTCCTTCAG GCAGATCGCA TGAGCCTGTT CATGTACAGG 
GTCCGAAATG GCATCGCAGA GCTAGCCACC CGGCTCTTCA ATGTCCACAA GGATGCTGTC 
CTTGAGGAAT GCCTGGTGGC GCCCGACTCA GAGATTGTGT TCCCCCTGGA CATGGGTGTG 
GTGGGTCACG TTGCCCACTC TAAAAAGATC GCCAACGTCG TCAACACAGA AGAGGATGAG 
CATTTCTGTG ACTTTGTGGA CACCCTCACT GAGTACCAGA CCAAGAACAT CCTGGCTTCC 
CCCATAATGA ATGGGAAGGA CGTAGTGGCA GTAATCATGG CTGTGAATAA AGTGGACGAG 
CCCCACTTCA CCAAGAGAGA TGAAGAGATT CTTCTCAAGT ACCTCAATTT TGCAAACCTA 
ATCATGAAGG TATACCACCT GAGTTACCTA CACAATTGCG AGACTCGGCG TGGCCAGATA 
CTGCTGTGGT CTGGGAGCAA AGTCTTTGAA GAGCTTACGG ACATCGAGAG GCAGTTCCAC 
AAGGCCCTGT ACACAGTCCG GGCCTTCCTC AACTGTGACA GATATTCTGT GGGACTCTTA 
GACATGACCA AGCAGAAGGA ATTTTTTGAT GTGTGGCCAG TCCTGATGGG GGAGGCTCCA 
CCCTATTCTG GTCCCAGGAC TCCGGATGGA AGGGAAATCA ACTTTTACAA GGTCATTGAC 
TATATCCTAC ACGGCAAAGA AGACATCAAA GTAATCCCGA ATCCACCTCC TGATCATTGG 
GCTTTAGTAA GTGGTCTGCC CACTTATGTT GCCCAGAATG GCCTGATTTG CAACATCATG 
AATGCACCTG CAGAGGACTT TTTTGCATTC CAGAAAGAGC CTCTGGATGA GTCTGGATGG 
ATGATTAAGA ATGTCCTTTC TTTGCCAATT GTGAACAAGA AGGAGGAAAT TGTTGGGGTG 
GCCACGTTTT ACAATCGCAA AGATGGAAAA CCCTTTGATG AAATGGATGA GACCCTCATG 
GAGTCTTTGG CTCAATTCCT GGGCTGGTCC GTCTTAAATC CTGATACTTA CGAGTCAATG 
AACAGACTTG AAAACAGGAA GGATATTTTC CAGGACATGG TAAAATACCA CGTGAAGTGT 
GACAATGAAG AGATCCAGAA AATCCTGAAA ACCAGAGAGG TGTATGGGAA GGAGCCGTGG 
GAGTGCGAGG AAGAGGAACT CGCTGAGATC CTGCAAGGAG AGCTGCCAGA TGCAGAGAAA 
TATGAAATCA ATAAATTCCA CTTCAGCGAC TTGCCCCTGA CCGAACTGGA GCTGGTGAAA 
TGTGGGATAC AGATGTACTA TGAGCTCAAA GTGGTGGATA AATTTCACAT TCCTCAGGAG 
GCCCTGGTGC GCTTCATGTA CTCGCTGAGC AAGGGCTACC GCAGGATCAC CTACCACAAC 
TGGCGGCACG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC CGGAAAGCTG 
AAGCGATACT TCACAGACCT AGAGGCCTTG GCCATGGTCA CCGCTGCCTT CTGCCATGAC 
ATTGACCACA GAGGCACCAA CAATCTCTAC CAGATGAAGT CCCAGAACCC ACTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGACAC CACTTGGAGT TCGGCAAAAC GTTGCTGCGA 
GATGAGAGCC TGAATATCTT TCAAAACCTC AATCGCAGGC AGCACGAGCA CGCCATCCAC 
ATGATGGACA TAGCAATCAT TGCCACAGAC CTCGCCCTGT ATTTCAAGAA GAGGACAATG 
TTCCAAAAGA TCGTGGATCA GTCTAAAACA TATGAAACTC AGCAGGAGTG GACACAGTAC 
ATGATGCTGG AGCAGACACG GAAGGAAATT GTTATGGCCA TGATGATGAC CGCCTGTGAT 
CTCTCAGCCA TCACCAAGCC CTGGGAGGTG CAGAGTAAGG TAGCTCTACT GGTTGCTGCC 
GAATTCTGGG AACAAGGTGA CCTGGAGCGC ACAGTGCTGC AGCAGAATCC CATTCCCATG 
ATGGACAGGA ACAAGGCAGA TGAACTCCCC AAGCTTCAAG TCGGCTTCAT TGACTTTGTT 
TGCACCTTTG TCTACAAGGA ATTCTCCCGT TTCCACGAGG AGATCACTCC CATGCTGGAT 
GGGATCACCA ACAACCGCAA GGAGTGGAAG GCGCTCGCCG ATGAGTACGA CACCAAGATG 
AAGGCCCTGG AGGAGGAGAA GCAGAAGCAG CAGACAGCCA AGCAAGGGGC GGCAGGAGAT 
CAGCCGGGGG GCAACCCCAG CCCGGCCGGG GGCGCACCTG CATCCAAGTC CTGCTGCATC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003073.1
CDS	106..2691
Translation	MGEVTAEQVEKFLDSNIIFAKQYYNLRYRAKVISDMLGAKEAAVDFSNYHSLSSVEESEIIFDLLRDFQENLQAERCIFNVMKKLCFLLQADRMSLFMYRVRNGIAELATRLFNVHKDAVLEECLVAPDSEIVFPLDMGVVGHVAHSKKIANVVNTEEDEHFCDFVDTLTEYQTKNILASPIMNGKDVVAVIMAVNKVDEPHFTKRDEEILLKYLNFANLIMKVYHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKQKEFFDVWPVLMGEAPPYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPPDHWALVSGLPTYVAQNGLICNIMNAPAEDFFAFQKEPLDESGWMIKNVLSLPIVNKKEEIVGVATFYNRKDGKPFDEMDETLMESLAQFLGWSVLNPDTYESMNRLENRKDIFQDMVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEILQGELPDAEKYEINKFHFSDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYETQQEWTQYMMLEQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYDTKMKALEEEKQKQQTAKQGAAGDQPGGNPSPAGGAPASKSCCIQ

Target ORF information:

RefSeq Version	NM_001003073.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphodiesterase 6A (PDE6A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003073.1

ORF Insert Sequence:

ATGGGTGAGG TGACAGCAGA GCAGGTGGAG AAGTTCCTGG ACTCGAATAT TATCTTTGCC 
AAACAGTACT ACAACCTCCG CTACCGGGCC AAGGTCATCT CAGACATGCT GGGGGCCAAG 
GAGGCAGCGG TGGACTTCAG CAACTACCAC TCGCTGAGCA GTGTGGAGGA GAGTGAAATC 
ATCTTTGACC TCCTGCGAGA CTTCCAGGAG AATTTGCAGG CCGAGAGATG CATCTTCAAT 
GTCATGAAGA AGCTGTGCTT CCTCCTTCAG GCAGATCGCA TGAGCCTGTT CATGTACAGG 
GTCCGAAATG GCATCGCAGA GCTAGCCACC CGGCTCTTCA ATGTCCACAA GGATGCTGTC 
CTTGAGGAAT GCCTGGTGGC GCCCGACTCA GAGATTGTGT TCCCCCTGGA CATGGGTGTG 
GTGGGTCACG TTGCCCACTC TAAAAAGATC GCCAACGTCG TCAACACAGA AGAGGATGAG 
CATTTCTGTG ACTTTGTGGA CACCCTCACT GAGTACCAGA CCAAGAACAT CCTGGCTTCC 
CCCATAATGA ATGGGAAGGA CGTAGTGGCA GTAATCATGG CTGTGAATAA AGTGGACGAG 
CCCCACTTCA CCAAGAGAGA TGAAGAGATT CTTCTCAAGT ACCTCAATTT TGCAAACCTA 
ATCATGAAGG TATACCACCT GAGTTACCTA CACAATTGCG AGACTCGGCG TGGCCAGATA 
CTGCTGTGGT CTGGGAGCAA AGTCTTTGAA GAGCTTACGG ACATCGAGAG GCAGTTCCAC 
AAGGCCCTGT ACACAGTCCG GGCCTTCCTC AACTGTGACA GATATTCTGT GGGACTCTTA 
GACATGACCA AGCAGAAGGA ATTTTTTGAT GTGTGGCCAG TCCTGATGGG GGAGGCTCCA 
CCCTATTCTG GTCCCAGGAC TCCGGATGGA AGGGAAATCA ACTTTTACAA GGTCATTGAC 
TATATCCTAC ACGGCAAAGA AGACATCAAA GTAATCCCGA ATCCACCTCC TGATCATTGG 
GCTTTAGTAA GTGGTCTGCC CACTTATGTT GCCCAGAATG GCCTGATTTG CAACATCATG 
AATGCACCTG CAGAGGACTT TTTTGCATTC CAGAAAGAGC CTCTGGATGA GTCTGGATGG 
ATGATTAAGA ATGTCCTTTC TTTGCCAATT GTGAACAAGA AGGAGGAAAT TGTTGGGGTG 
GCCACGTTTT ACAATCGCAA AGATGGAAAA CCCTTTGATG AAATGGATGA GACCCTCATG 
GAGTCTTTGG CTCAATTCCT GGGCTGGTCC GTCTTAAATC CTGATACTTA CGAGTCAATG 
AACAGACTTG AAAACAGGAA GGATATTTTC CAGGACATGG TAAAATACCA CGTGAAGTGT 
GACAATGAAG AGATCCAGAA AATCCTGAAA ACCAGAGAGG TGTATGGGAA GGAGCCGTGG 
GAGTGCGAGG AAGAGGAACT CGCTGAGATC CTGCAAGGAG AGCTGCCAGA TGCAGAGAAA 
TATGAAATCA ATAAATTCCA CTTCAGCGAC TTGCCCCTGA CCGAACTGGA GCTGGTGAAA 
TGTGGGATAC AGATGTACTA TGAGCTCAAA GTGGTGGATA AATTTCACAT TCCTCAGGAG 
GCCCTGGTGC GCTTCATGTA CTCGCTGAGC AAGGGCTACC GCAGGATCAC CTACCACAAC 
TGGCGGCACG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC CGGAAAGCTG 
AAGCGATACT TCACAGACCT AGAGGCCTTG GCCATGGTCA CCGCTGCCTT CTGCCATGAC 
ATTGACCACA GAGGCACCAA CAATCTCTAC CAGATGAAGT CCCAGAACCC ACTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGACAC CACTTGGAGT TCGGCAAAAC GTTGCTGCGA 
GATGAGAGCC TGAATATCTT TCAAAACCTC AATCGCAGGC AGCACGAGCA CGCCATCCAC 
ATGATGGACA TAGCAATCAT TGCCACAGAC CTCGCCCTGT ATTTCAAGAA GAGGACAATG 
TTCCAAAAGA TCGTGGATCA GTCTAAAACA TATGAAACTC AGCAGGAGTG GACACAGTAC 
ATGATGCTGG AGCAGACACG GAAGGAAATT GTTATGGCCA TGATGATGAC CGCCTGTGAT 
CTCTCAGCCA TCACCAAGCC CTGGGAGGTG CAGAGTAAGG TAGCTCTACT GGTTGCTGCC 
GAATTCTGGG AACAAGGTGA CCTGGAGCGC ACAGTGCTGC AGCAGAATCC CATTCCCATG 
ATGGACAGGA ACAAGGCAGA TGAACTCCCC AAGCTTCAAG TCGGCTTCAT TGACTTTGTT 
TGCACCTTTG TCTACAAGGA ATTCTCCCGT TTCCACGAGG AGATCACTCC CATGCTGGAT 
GGGATCACCA ACAACCGCAA GGAGTGGAAG GCGCTCGCCG ATGAGTACGA CACCAAGATG 
AAGGCCCTGG AGGAGGAGAA GCAGAAGCAG CAGACAGCCA AGCAAGGGGC GGCAGGAGAT 
CAGCCGGGGG GCAACCCCAG CCCGGCCGGG GGCGCACCTG CATCCAAGTC CTGCTGCATC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6A ( XM_005618863.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005618920.1 Canis lupus familiaris phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe04855
Clone ID Related Accession (Same CDS sequence)	XM_005618863.2
Accession Version	XM_005618863.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618863.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG CCAAGGAGGC AGCGGTGGAC TTCAGCAACT ACCACTCGCT GAGCAGTGTG 
GAGGAGAGTG AAATCATCTT TGACCTCCTG CGAGACTTCC AGGAGAATTT GCAGGCCGAG 
AGATGCATCT TCAATGTCAT GAAGAAGCTG TGCTTCCTCC TTCAGGCAGA TCGCATGAGC 
CTGTTCATGT ACAGGGTCCG AAATGGCATC GCAGAGCTAG CCACCCGGCT CTTCAATGTC 
CACAAGGATG CTGTCCTTGA GGAATGCCTG GTGGCGCCCG ACTCAGAGAT TGTGTTCCCC 
CTGGACATGG GTGTGGTGGG TCACGTTGCC CACTCTAAAA AGATCGCCAA CGTCGTCAAC 
ACAGAAGAGG ATGAGCATTT CTGTGACTTT GTGGACACCC TCACTGAGTA CCAGACCAAG 
AACATCTTGG CTTCCCCCAT AATGAATGGG AAGGACGTAG TGGCAGTAAT CATGGCTGTG 
AATAAAGTGG ACGAGCCCCA CTTCACCAAG AGAGATGAAG AGATTCTTCT CAAGTACCTC 
AATTTTGCAA ACCTAATCAT GAAGGTATAC CACCTGAGTT ACCTACACAA TTGCGAGACT 
CGGCGTGGCC AGATACTGCT GTGGTCTGGG AGCAAAGTCT TTGAAGAGCT TACGGACATC 
GAGAGGCAGT TCCACAAGGC CCTGTACACA GTCCGGGCCT TCCTCAACTG TGACAGATAT 
TCTGTGGGAC TCTTAGACAT GACCAAGCAG AAGGAATTTT TTGATGTGTG GCCAGTCCTG 
ATGGGGGAGG CTCCACCCTA TTCTGGTCCC AGGACTCCGG ATGGAAGGGA AATCAACTTT 
TACAAGGTCA TTGACTATAT CCTACACGGC AAAGAAGACA TCAAAGTAAT CCCGAATCCA 
CCTCCTGATC ATTGGGCTTT AGTAAGTGGT CTGCCCACTT ATGTTGCCCA GAATGGCCTG 
ATTTGCAACA TCATGAATGC ACCTGCAGAG GACTTTTTTG CATTCCAGAA AGAGCCTCTG 
GATGAGTCTG GATGGATGAT TAAGAATGTC CTTTCTATGC CAATTGTGAA CAAGAAGGAG 
GAAATTGTTG GGGTGGCCAC GTTTTACAAT CGCAAAGATG GAAAACCCTT TGATGAAATG 
GATGAGACCC TCATGGAGTC TTTGGCTCAA TTCCTGGGCT GGTCCGTCTT AAATCCTGAT 
ACTTACGAGT CAATGAACAG ACTTGAAAAC AGGAAGGATA TTTTCCAGGA CATGGTAAAA 
TACCACGTGA AGTGTGACAA TGAAGAGATC CAGAAAATCC TGAAAACCAG AGAGGTGTAT 
GGGAAGGAGC CGTGGGAGTG CGAGGAAGAG GAACTCGCTG AGATCCTGCA AGGAGAGCTG 
CCAGATGCAG AGAAATATGA AATCAATAAA TTCCACTTCA GCGACTTGCC CCTGACCGAA 
CTGGAGCTGG TGAAATGTGG GATACAGATG TACTATGAGC TCAAAGTGGT GGATAAATTT 
CACATTCCTC AGGAGGCCCT GGTGCGCTTC ATGTACTCGC TGAGCAAGGG CTACCGCAGG 
ATCACCTACC ACAACTGGCG GCACGGCTTC AACGTGGGGC AGACCATGTT CTCCTTGCTG 
GTGACCGGAA AGCTGAAGCG ATACTTCACA GACCTAGAGG CCTTGGCCAT GGTCACCGCT 
GCCTTCTGCC ATGACATTGA CCACAGAGGC ACCAACAATC TCTACCAGAT GAAGTCCCAG 
AACCCACTGG CCAAGCTCCA TGGGTCCTCC ATCTTGGAAA GACACCACTT GGAGTTCGGC 
AAAACGTTGC TGCGAGATGA GAGCCTGAAT ATCTTTCAAA ACCTCAATCG CAGGCAGCAC 
GAGCACGCCA TCCACATGAT GGACATAGCA ATCATTGCCA CAGACCTCGC CCTGTATTTC 
AAGAAGAGGA CAATGTTCCA AAAGATCGTG GATCAGTCTA AAACATATGA AACTCAGCAG 
GAGTGGACAC AGTACATGAT GCTGGAGCAG ACACGGAAGG AAATTGTTAT GGCCATGATG 
ATGACCGCCT GTGATCTCTC AGCCATCACC AAGCCCTGGG AGGTGCAGAG TAAGGTAGCT 
CTACTGGTTG CTGCCGAATT CTGGGAACAA GGTGACCTGG AGCGCACAGT GCTGCAGCAG 
AATCCCATTC CCATGATGGA CAGGAACAAG GCAGATGAAC TCCCCAAGCT TCAAGTCGGC 
TTCATTGACT TTGTTTGCAC CTTTGTCTAC AAGGAATTCT CCCGTTTCCA CGAGGAGATC 
ACTCCCATGC TGGATGGGAT CACCAACAAC CGCAAGGAGT GGAAGGCGCT CGCCGATGAG 
TACGACACCA AGATGAAGGC CCTGGAGGAG GAGAAGCAGA AGCAGCAGAC AGCCAAGCAA 
GGGGCGGCAG GAGATCAGCC GGGGGGCAAC CCCAGCCCGG CCGGGGGCGC ACCTGCATCC 
AAGTCCTGCT GCATCCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618920.1
CDS	81..2561
Translation	MLGAKEAAVDFSNYHSLSSVEESEIIFDLLRDFQENLQAERCIFNVMKKLCFLLQADRMSLFMYRVRNGIAELATRLFNVHKDAVLEECLVAPDSEIVFPLDMGVVGHVAHSKKIANVVNTEEDEHFCDFVDTLTEYQTKNILASPIMNGKDVVAVIMAVNKVDEPHFTKRDEEILLKYLNFANLIMKVYHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKQKEFFDVWPVLMGEAPPYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPPDHWALVSGLPTYVAQNGLICNIMNAPAEDFFAFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEMDETLMESLAQFLGWSVLNPDTYESMNRLENRKDIFQDMVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEILQGELPDAEKYEINKFHFSDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYETQQEWTQYMMLEQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYDTKMKALEEEKQKQQTAKQGAAGDQPGGNPSPAGGAPASKSCCIQ

Target ORF information:

RefSeq Version	XM_005618863.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618863.2

ORF Insert Sequence:

ATGCTGGGGG CCAAGGAGGC AGCGGTGGAC TTCAGCAACT ACCACTCGCT GAGCAGTGTG 
GAGGAGAGTG AAATCATCTT TGACCTCCTG CGAGACTTCC AGGAGAATTT GCAGGCCGAG 
AGATGCATCT TCAATGTCAT GAAGAAGCTG TGCTTCCTCC TTCAGGCAGA TCGCATGAGC 
CTGTTCATGT ACAGGGTCCG AAATGGCATC GCAGAGCTAG CCACCCGGCT CTTCAATGTC 
CACAAGGATG CTGTCCTTGA GGAATGCCTG GTGGCGCCCG ACTCAGAGAT TGTGTTCCCC 
CTGGACATGG GTGTGGTGGG TCACGTTGCC CACTCTAAAA AGATCGCCAA CGTCGTCAAC 
ACAGAAGAGG ATGAGCATTT CTGTGACTTT GTGGACACCC TCACTGAGTA CCAGACCAAG 
AACATCTTGG CTTCCCCCAT AATGAATGGG AAGGACGTAG TGGCAGTAAT CATGGCTGTG 
AATAAAGTGG ACGAGCCCCA CTTCACCAAG AGAGATGAAG AGATTCTTCT CAAGTACCTC 
AATTTTGCAA ACCTAATCAT GAAGGTATAC CACCTGAGTT ACCTACACAA TTGCGAGACT 
CGGCGTGGCC AGATACTGCT GTGGTCTGGG AGCAAAGTCT TTGAAGAGCT TACGGACATC 
GAGAGGCAGT TCCACAAGGC CCTGTACACA GTCCGGGCCT TCCTCAACTG TGACAGATAT 
TCTGTGGGAC TCTTAGACAT GACCAAGCAG AAGGAATTTT TTGATGTGTG GCCAGTCCTG 
ATGGGGGAGG CTCCACCCTA TTCTGGTCCC AGGACTCCGG ATGGAAGGGA AATCAACTTT 
TACAAGGTCA TTGACTATAT CCTACACGGC AAAGAAGACA TCAAAGTAAT CCCGAATCCA 
CCTCCTGATC ATTGGGCTTT AGTAAGTGGT CTGCCCACTT ATGTTGCCCA GAATGGCCTG 
ATTTGCAACA TCATGAATGC ACCTGCAGAG GACTTTTTTG CATTCCAGAA AGAGCCTCTG 
GATGAGTCTG GATGGATGAT TAAGAATGTC CTTTCTATGC CAATTGTGAA CAAGAAGGAG 
GAAATTGTTG GGGTGGCCAC GTTTTACAAT CGCAAAGATG GAAAACCCTT TGATGAAATG 
GATGAGACCC TCATGGAGTC TTTGGCTCAA TTCCTGGGCT GGTCCGTCTT AAATCCTGAT 
ACTTACGAGT CAATGAACAG ACTTGAAAAC AGGAAGGATA TTTTCCAGGA CATGGTAAAA 
TACCACGTGA AGTGTGACAA TGAAGAGATC CAGAAAATCC TGAAAACCAG AGAGGTGTAT 
GGGAAGGAGC CGTGGGAGTG CGAGGAAGAG GAACTCGCTG AGATCCTGCA AGGAGAGCTG 
CCAGATGCAG AGAAATATGA AATCAATAAA TTCCACTTCA GCGACTTGCC CCTGACCGAA 
CTGGAGCTGG TGAAATGTGG GATACAGATG TACTATGAGC TCAAAGTGGT GGATAAATTT 
CACATTCCTC AGGAGGCCCT GGTGCGCTTC ATGTACTCGC TGAGCAAGGG CTACCGCAGG 
ATCACCTACC ACAACTGGCG GCACGGCTTC AACGTGGGGC AGACCATGTT CTCCTTGCTG 
GTGACCGGAA AGCTGAAGCG ATACTTCACA GACCTAGAGG CCTTGGCCAT GGTCACCGCT 
GCCTTCTGCC ATGACATTGA CCACAGAGGC ACCAACAATC TCTACCAGAT GAAGTCCCAG 
AACCCACTGG CCAAGCTCCA TGGGTCCTCC ATCTTGGAAA GACACCACTT GGAGTTCGGC 
AAAACGTTGC TGCGAGATGA GAGCCTGAAT ATCTTTCAAA ACCTCAATCG CAGGCAGCAC 
GAGCACGCCA TCCACATGAT GGACATAGCA ATCATTGCCA CAGACCTCGC CCTGTATTTC 
AAGAAGAGGA CAATGTTCCA AAAGATCGTG GATCAGTCTA AAACATATGA AACTCAGCAG 
GAGTGGACAC AGTACATGAT GCTGGAGCAG ACACGGAAGG AAATTGTTAT GGCCATGATG 
ATGACCGCCT GTGATCTCTC AGCCATCACC AAGCCCTGGG AGGTGCAGAG TAAGGTAGCT 
CTACTGGTTG CTGCCGAATT CTGGGAACAA GGTGACCTGG AGCGCACAGT GCTGCAGCAG 
AATCCCATTC CCATGATGGA CAGGAACAAG GCAGATGAAC TCCCCAAGCT TCAAGTCGGC 
TTCATTGACT TTGTTTGCAC CTTTGTCTAC AAGGAATTCT CCCGTTTCCA CGAGGAGATC 
ACTCCCATGC TGGATGGGAT CACCAACAAC CGCAAGGAGT GGAAGGCGCT CGCCGATGAG 
TACGACACCA AGATGAAGGC CCTGGAGGAG GAGAAGCAGA AGCAGCAGAC AGCCAAGCAA 
GGGGCGGCAG GAGATCAGCC GGGGGGCAAC CCCAGCCCGG CCGGGGGCGC ACCTGCATCC 
AAGTCCTGCT GCATCCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of a canine model of autosomal recessive retinitis pigmentosa due to a PDE6A mutation.
Investigative ophthalmology & visual science50(2)801-13(2009 Feb)
Tuntivanich N,Pittler SJ,Fischer AJ,Omar G,Kiupel M,Weber A,Yao S,Steibel JP,Khan NW,Petersen-Jones SM

Exclusion of the PDE6A gene for generalised progressive retinal atrophy in 11 breeds of dog.
Animal genetics31(2)135-9(2000 Apr)
Dekomien G,Epplen JT

cGMP phosphodiesterase-alpha mutation causes progressive retinal atrophy in the Cardigan Welsh corgi dog.
Investigative ophthalmology & visual science40(8)1637-44(1999 Jul)
Petersen-Jones SM,Entz DD,Sargan DR

Microsatellite (CA)n polymorphism in intron 2 of the canine rod photoreceptor cGMP-phosphodiesterase alpha-subunit gene.
Animal genetics28(5)380(1997 Oct)
Veske A,Nilsson SE,Gal A

Cloning of the cDNA encoding rod photoreceptor cGMP-phosphodiesterase alpha and gamma subunits from the retinal degenerate Labrador retriever dog.
Research in veterinary science62(3)293-6(1997 May-Jun)
Kylmä T,Paulin L,Hurwitz MY,Hurwitz RL,Kommonen B