CENPE cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CENPE
Entrez Gene ID	100855663
Full Name	centromere protein E
General protein information	Preferred Names centromere protein E Names centromere-associated protein E centromere protein E, 312kDa
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014110057.1	XP_013965532.1	centromere-associated protein E isoform X1
XM_014110058.1	XP_013965533.1	centromere-associated protein E isoform X2
XM_014110058.2	XP_013965533.1	centromere-associated protein E isoform X3
XM_022413494.1	XP_022269202.1	centromere-associated protein E isoform X2
XM_022413495.1	XP_022269203.1	centromere-associated protein E isoform X4
XM_014110057.2	XP_013965532.1	centromere-associated protein E isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CENPE	XP_002688141.1
Macaca mulatta (Rhesus monkey)	CENPE	XP_001110512.1
Canis lupus familiaris (dog)	CENPE	XP_005639295.1
Homo sapiens (human)	CENPE	NP_001804.2
Pan troglodytes (chimpanzee)	CENPE	XP_001170275.1
Mus musculus (house mouse)	Cenpe	NP_776123.3
Rattus norvegicus (Norway rat)	Cenpe	XP_006233412.1

Gene Ontology

The following CENPE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CENPE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe284063	XM_022413495.1 Latest version!	Canis lupus familiaris centromere protein E (CENPE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe44623	XM_014110058.2 Latest version!	Canis lupus familiaris centromere protein E (CENPE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe284062	XM_022413494.1 Latest version!	Canis lupus familiaris centromere protein E (CENPE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe44622	XM_014110057.2 Latest version!	Canis lupus familiaris centromere protein E (CENPE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OCe44623	XM_014110058.1	Canis lupus familiaris centromere protein E, 312kDa (CENPE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe44622	XM_014110057.1	Canis lupus familiaris centromere protein E, 312kDa (CENPE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CENPE ( XM_022413495.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269203.1 Canis lupus familiaris centromere protein E (CENPE), transcript variant X4, mRNA.

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CloneID	OCe284063
Clone ID Related Accession (Same CDS sequence)	XM_022413495.1
Accession Version	XM_022413495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	centromere-associated protein E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGACCA ACAGAACCAA 
AAAGAGATAG TAAAATATGA AAAAAGGTTA CAATGTAATG AAAAACACCA CCTCACAGAA 
AGCCTAAGGG AGAAGTGCTC TAGGATAAAA GAGCTTCTGA AGAGATACTC AGAGATGTAT 
AATCATTATG AGTGCTTAAC TAGACTATCC CTTGACTTGG AGAAAGAAAT TGAAACCCAA 
AAAGAGCTTT CAGTTACAAT AAAAGCAAAC CTCTCACTTC CACATTCACA AATCAAACAA 
ATTGAAAAAC TTCTCGCTGT AAACCAAAGA TGTTCAATGG AATTCCACAG AATCATCAAG 
AGATTTCAGT ACGTGTTAAG CCAGATTACA AAGATAAAGG AAGAACAACA TGAATCCATA 
AATAAATTAG AAGTGGTTTT TATTGATGAA GTGGAAAAGC ACAATGAACT GGTAATTAAA 
ATACAGCACC TTCAACAAGA TTTTAATGTA CCATCTGGAG AATTAACGGA CCTCAAATTG 
AGCCAGAAGA TGGATCTACG TATTGAGGAA ATTCTCAAAG ATCTTTCAGA AAATGATTTC 
CACTTACTAA AGACTGAATT TCAACAAGTG CTAAGTAATA GGAGAGAAAT AACCCAGTTT 
TTGGAAGAAT GGTTGAATAC TCATTTTGAT ATAGAAAAGC TTAAAAATGG CATCCAGAAA 
GAAAATGATA ATATTTGTCA AGTGAATAAC TTCTATAATA ACAAAATAAT TGCTATAATG 
AATGAATCTA CAGAGTTTGA GGAAAGATAT GCCATCATAG CCAAAGAATG GGAACGTGAC 
CTGAATTCAG TGAAAGAGAA AAATGAACAA CTATTTAAAA ACTATCAAAC TTTGAAGCTC 
ACCCGGACGT CTGGTATCCC GGTAAATTCT GCCACAGAAG ACAATAAGAA TCTTCATGTT 
ACATCAGGAG CCACACAACT AACCACAGAG AAAATTCAGG AGCTGGAAGC TTCACTGCAT 
GAAGCTAAAG AAAGTGCTTT GCACAAGGAA AACAAGATTG TAAAGATGCA GAAAGAACTT 
GAGATGACTA ATGACATCAT AGCAAAACTT CAGTCGCAAG TTAATGAATC AAATAAATGC 
CTTGAAAAAA CAAAAGAAAG GATCCAATTA CTTCAGGATA AAGTTGCTTT AGGAGCTAAG 
CCCTATAAAG AAGAAATTGA AGATCTCAAG ATGAAACTGG TGAAAATGGA TCTAGAAAAG 
AAGAAGAATG CCAAAGAGTT TGAAAAAGAA ATTACTTCTA CAAAGGCCAC TGTAGAACAT 
CAAAAAGAAG TTATAAGGGT ACTGAGAGAA AATCTCAGAA GAAATCAACA AGCCCAGGAT 
ACCTCAATTG TATCAGAATA TACGGACTCT CAGCCTCCAA ATAAACCCTT AACTTGTGGA 
GGTGGCAGTG GCATCGTACA AAGCACAAAA GCTCTTATTT TAAAAAGTGG ATATATACGG 
CTAGAAAAGG AAATCTCTAA GTTAAAACAG CAAAATGAAC AGCTAATAAA GCAAAAGAAT 
GAACTATTAA GCAATAACCA TCATCTTTCC AACGAAGTCA AAACATGGAA GGAGAGAATC 
CTTAAAAGAG AGGCTCACAG AGAAGTTACT TGTGAGAATT CTCCACAGTC TCCTAAAGTG 
ACTATAACAG CTCCCAAAAA GAGGCCGCAT ACGCCTTCTC AATGCAAGGA ACGGAATTTA 
CAAGATCCTG TGCCAAGGGA ATCGCCAAAG TCTTGGTTTT TTGACAGCCG ATCAAAGTCT 
TTACCATCAC CTCATCCAGT TCGCTACTTT GATAACTCGG GTTTAGGCCT TTGCCCAGAA 
GAACAAATTG CTGGAGCAGA GAATGTGGAT CCTCAGCCTG GTCTATGGCA TGCCTCCTCA 
GGAAAAGATG TGCCCGAGTG CAAGACCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269203.1
CDS	99..7931
Translation	MAEEGAVAVCVRVRPLNDREEALGGDTQIYWKTDNNAIYQVDGSKSFNFDRVFHSNETTKHVYEEIAVPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSQDYLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCDTQKMKPLIIREDFNRNVYVADLTEEVVYTSDMALKWITKGEKNRHYGITKMNQRSSRSHTIFRMILESREKGEPSNCDGSVKVSHLNLVDLAGSERAAQTGAEGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTVTPVSFDETLTTLQFASTAKYMKNTPYVNEVSSDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQTEKIQNLTRMLVTSSSLTSHQELKAKRKRRVTWCLGKINKMKDSNYINEFNMSTNITTRKAALTVVGEIDESLCSESDVFSNTFDTLNETEWNPATKLLSQENLESELNSLRANYDNLVLDYEQLRQEHEEMELKLKEKNDLDEFEALERKAEKDQEMQLIHEISNLKNLVKHAEVYNQDLENELSSKVELLREKEDQIKKLQKYIDSQKSEDIKTDLSYSSESTEDLKQMKQTLLDAETVVLDAKRESAFLRSENLELKEKMKELRSRYKQMENDIQLYQRQLEAKKKMQVDLEKELQSSFNEITKLTSLIDGKVPKGLLCNLELERKITDFQRELNEKVEENEALHKEVKLLSALKSLPSEIEMLRKEIHDKSEELYIITSEREKLNSEVVQKESRIQDLLEEIGKTKNDLATIRLNYESTDQEFQDFKNHHIELEQKYKMVLEENARMNQELGNLSEQAQKLGLSLDALSNTELSPKSEELLQKTTESQKRLNEVEELKEQLESTGSRLQTIEKEKTLIAEQLQQTLVEVRTLTQEKDDLKQLQESLQIERDQLKSDIQDTVNMNIDTQEQLRNALEALKQHQEVINTLRLKIAEKTSRHLETEENLGEAKDEFQEEMVDTDKKQNLKAKNTQALVVNVEDNELTEQQRNIFSLIQEKNELQRMLESVTAEKEQLKTDLRENIEMTIENQEELRILGDELKKQQEILVQEKNHTIKKEEELSRTYKKLAEVEEKLKEKNQQLQEKQLQLLSVQEEMSEMQKKMNEMENVNNEFRNRELTLERIEMERLEVAQKLHENDEEMKSLTKERNDLKELQESFELEREQLEGFIREIEVTIPVLHEEQELLPNVKEVSATQGTMNEGEILKKQSKTKDSMTPARIEIERLGLTAKLPESHEEIKSLTEERDNLKTIKEAVQVGQEQLKEDSREMLAEDSLNISEPQNKQEQSFDMKEREDEAEEIVSELEQLQEQLKAKEAPQPRVGMERLEMSEKLQEVLQSETNQLKENMREIIAKHLETEEELKVARCHLKEQEETIDKLRVNLSERETELSSLQKELEITNNELQKKIQELHEKQQFMSTKEITETQEKMSELEQLKEQLRVKDSSLQNIESEKLKLTEKLQESQDKIKIVIKERDELKRVQELLQMERDQLKANIKEIVAEEYQQTISTRNISKKTDQEANIERVIESSDAESQEKIQELQEKEHQLLKMKTVSETQEKICEMEHLKNQFEAQKSTLENIEMENARLTQRLHENLEEMRSVTKERDDLRSIEETLKVERDQLKENLRETVIRDLEKQEELRIARMHVKEHQETIDKLRGLVSEKTDELSNMQIDLENTNDALKTQIQELQEKEHQLLKVKNDLRENMYQTEQLKEQLQAQHSTLESVETERLRLSQKLHENLEEIKCVTEERDTLKRAERTLRMEQCQLRESLREKEAQDLEKQEELRIVCMHVKEHQETIDKLRGLVSEKTDEIANMQMDLENSNAKLQEKIQQLKANEHQLFKLKEDVSETQKKTSEIERLKKQLKGQSLTLDKIEMENLNLAQKLHENLEEMKSVMQERDNLKLERDQLKTNLQETIARDQQNQKEIVKYEKRLQCNEKHHLTESLREKCSRIKELLKRYSEMYNHYECLTRLSLDLEKEIETQKELSVTIKANLSLPHSQIKQIEKLLAVNQRCSMEFHRIIKRFQYVLSQITKIKEEQHESINKLEVVFIDEVEKHNELVIKIQHLQQDFNVPSGELTDLKLSQKMDLRIEEILKDLSENDFHLLKTEFQQVLSNRREITQFLEEWLNTHFDIEKLKNGIQKENDNICQVNNFYNNKIIAIMNESTEFEERYAIIAKEWERDLNSVKEKNEQLFKNYQTLKLTRTSGIPVNSATEDNKNLHVTSGATQLTTEKIQELEASLHEAKESALHKENKIVKMQKELEMTNDIIAKLQSQVNESNKCLEKTKERIQLLQDKVALGAKPYKEEIEDLKMKLVKMDLEKKKNAKEFEKEITSTKATVEHQKEVIRVLRENLRRNQQAQDTSIVSEYTDSQPPNKPLTCGGGSGIVQSTKALILKSGYIRLEKEISKLKQQNEQLIKQKNELLSNNHHLSNEVKTWKERILKREAHREVTCENSPQSPKVTITAPKKRPHTPSQCKERNLQDPVPRESPKSWFFDSRSKSLPSPHPVRYFDNSGLGLCPEEQIAGAENVDPQPGLWHASSGKDVPECKTQ

Target ORF information:

RefSeq Version	XM_022413495.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centromere protein E (CENPE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413495.1

ORF Insert Sequence:

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGACCA ACAGAACCAA 
AAAGAGATAG TAAAATATGA AAAAAGGTTA CAATGTAATG AAAAACACCA CCTCACAGAA 
AGCCTAAGGG AGAAGTGCTC TAGGATAAAA GAGCTTCTGA AGAGATACTC AGAGATGTAT 
AATCATTATG AGTGCTTAAC TAGACTATCC CTTGACTTGG AGAAAGAAAT TGAAACCCAA 
AAAGAGCTTT CAGTTACAAT AAAAGCAAAC CTCTCACTTC CACATTCACA AATCAAACAA 
ATTGAAAAAC TTCTCGCTGT AAACCAAAGA TGTTCAATGG AATTCCACAG AATCATCAAG 
AGATTTCAGT ACGTGTTAAG CCAGATTACA AAGATAAAGG AAGAACAACA TGAATCCATA 
AATAAATTAG AAGTGGTTTT TATTGATGAA GTGGAAAAGC ACAATGAACT GGTAATTAAA 
ATACAGCACC TTCAACAAGA TTTTAATGTA CCATCTGGAG AATTAACGGA CCTCAAATTG 
AGCCAGAAGA TGGATCTACG TATTGAGGAA ATTCTCAAAG ATCTTTCAGA AAATGATTTC 
CACTTACTAA AGACTGAATT TCAACAAGTG CTAAGTAATA GGAGAGAAAT AACCCAGTTT 
TTGGAAGAAT GGTTGAATAC TCATTTTGAT ATAGAAAAGC TTAAAAATGG CATCCAGAAA 
GAAAATGATA ATATTTGTCA AGTGAATAAC TTCTATAATA ACAAAATAAT TGCTATAATG 
AATGAATCTA CAGAGTTTGA GGAAAGATAT GCCATCATAG CCAAAGAATG GGAACGTGAC 
CTGAATTCAG TGAAAGAGAA AAATGAACAA CTATTTAAAA ACTATCAAAC TTTGAAGCTC 
ACCCGGACGT CTGGTATCCC GGTAAATTCT GCCACAGAAG ACAATAAGAA TCTTCATGTT 
ACATCAGGAG CCACACAACT AACCACAGAG AAAATTCAGG AGCTGGAAGC TTCACTGCAT 
GAAGCTAAAG AAAGTGCTTT GCACAAGGAA AACAAGATTG TAAAGATGCA GAAAGAACTT 
GAGATGACTA ATGACATCAT AGCAAAACTT CAGTCGCAAG TTAATGAATC AAATAAATGC 
CTTGAAAAAA CAAAAGAAAG GATCCAATTA CTTCAGGATA AAGTTGCTTT AGGAGCTAAG 
CCCTATAAAG AAGAAATTGA AGATCTCAAG ATGAAACTGG TGAAAATGGA TCTAGAAAAG 
AAGAAGAATG CCAAAGAGTT TGAAAAAGAA ATTACTTCTA CAAAGGCCAC TGTAGAACAT 
CAAAAAGAAG TTATAAGGGT ACTGAGAGAA AATCTCAGAA GAAATCAACA AGCCCAGGAT 
ACCTCAATTG TATCAGAATA TACGGACTCT CAGCCTCCAA ATAAACCCTT AACTTGTGGA 
GGTGGCAGTG GCATCGTACA AAGCACAAAA GCTCTTATTT TAAAAAGTGG ATATATACGG 
CTAGAAAAGG AAATCTCTAA GTTAAAACAG CAAAATGAAC AGCTAATAAA GCAAAAGAAT 
GAACTATTAA GCAATAACCA TCATCTTTCC AACGAAGTCA AAACATGGAA GGAGAGAATC 
CTTAAAAGAG AGGCTCACAG AGAAGTTACT TGTGAGAATT CTCCACAGTC TCCTAAAGTG 
ACTATAACAG CTCCCAAAAA GAGGCCGCAT ACGCCTTCTC AATGCAAGGA ACGGAATTTA 
CAAGATCCTG TGCCAAGGGA ATCGCCAAAG TCTTGGTTTT TTGACAGCCG ATCAAAGTCT 
TTACCATCAC CTCATCCAGT TCGCTACTTT GATAACTCGG GTTTAGGCCT TTGCCCAGAA 
GAACAAATTG CTGGAGCAGA GAATGTGGAT CCTCAGCCTG GTCTATGGCA TGCCTCCTCA 
GGAAAAGATG TGCCCGAGTG CAAGACCCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPE ( XM_014110058.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965533.1 Canis lupus familiaris centromere protein E, 312kDa (CENPE), transcript variant X2, mRNA.

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CloneID	OCe44623
Clone ID Related Accession (Same CDS sequence)	XM_014110058.1 , XM_014110058.2
Accession Version	XM_014110058.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	centromere-associated protein E isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGATTT GGAAACACAA 
CAAGAACTAA AAATTGCTCT TTTGCATTTG AAAGAACACC AAGAAACTAT TGATAAATTC 
AGAGAGAGAG TTTCAGAAAA GACAACTCAG ATTTCAAATA TCCAAAAGGA TTTAAATAAA 
TCTAAAGATG AGTTACAGAA AAAGGACCAA CAGAACCAAA AAGAGATAGT AAAATATGAA 
AAAAGGTTAC AATGTAATGA AAAACACCAC CTCACAGAAA GCCTAAGGGA GAAGTGCTCT 
AGGATAAAAG AGCTTCTGAA GAGATACTCA GAGATGTATA ATCATTATGA GTGCTTAACT 
AGACTATCCC TTGACTTGGA GAAAGAAATT GAAACCCAAA AAGAGCTTTC AGTTACAATA 
AAAGCAAACC TCTCACTTCC ACATTCACAA ATCAAACAAA TTGAAAAACT TCTCGCTGTA 
AACCAAAGAT GTTCAATGGA ATTCCACAGA ATCATCAAGA GATTTCAGTA CGTGTTAAGC 
CAGATTACAA AGATAAAGGA AGAACAACAT GAATCCATAA ATAAATTAGA AGTGGTTTTT 
ATTGATGAAG TGGAAAAGCA CAATGAACTG GTAATTAAAA TACAGCACCT TCAACAAGAT 
TTTAATGTAC CATCTGGAGA ATTAACGGAC CTCAAATTGA GCCAGAAGAT GGATCTACGT 
ATTGAGGAAA TTCTCAAAGA TCTTTCAGAA AATGATTTCC ACTTACTAAA GACTGAATTT 
CAACAAGTGC TAAGTAATAG GAGAGAAATA ACCCAGTTTT TGGAAGAATG GTTGAATACT 
CATTTTGATA TAGAAAAGCT TAAAAATGGC ATCCAGAAAG AAAATGATAA TATTTGTCAA 
GTGAATAACT TCTATAATAA CAAAATAATT GCTATAATGA ATGAATCTAC AGAGTTTGAG 
GAAAGATATG CCATCATAGC CAAAGAATGG GAACGTGACC TGAATTCAGT GAAAGAGAAA 
AATGAACAAC TATTTAAAAA CTATCAAACT TTGAAGCTCA CCCGGACGTC TGGTATCCCG 
GTAAATTCTG CCACAGAAGA CAATAAGAAT CTTCATGTTA CATCAGGAGC CACACAACTA 
ACCACAGAGA AAATTCAGGA GCTGGAAGCT TCACTGCATG AAGCTAAAGA AAGTGCTTTG 
CACAAGGAAA ACAAGATTGT AAAGATGCAG AAAGAACTTG AGATGACTAA TGACATCATA 
GCAAAACTTC AGTCGCAAGT TAATGAATCA AATAAATGCC TTGAAAAAAC AAAAGAAAGG 
ATCCAATTAC TTCAGGATAA AGTTGCTTTA GGAGCTAAGC CCTATAAAGA AGAAATTGAA 
GATCTCAAGA TGAAACTGGT GAAAATGGAT CTAGAAAAGA AGAAGAATGC CAAAGAGTTT 
GAAAAAGAAA TTACTTCTAC AAAGGCCACT GTAGAACATC AAAAAGAAGT TATAAGGGTA 
CTGAGAGAAA ATCTCAGAAG AAATCAACAA GCCCAGGATA CCTCAATTGT ATCAGAATAT 
ACGGACTCTC AGCCTCCAAA TAAACCCTTA ACTTGTGGAG GTGGCAGTGG CATCGTACAA 
AGCACAAAAG CTCTTATTTT AAAAAGTGGA TATATACGGC TAGAAAAGGA AATCTCTAAG 
TTAAAACAGC AAAATGAACA GCTAATAAAG CAAAAGAATG AACTATTAAG CAATAACCAT 
CATCTTTCCA ACGAAGTCAA AACATGGAAG GAGAGAATCC TTAAAAGAGA GGCTCACAGA 
GAAGTTACTT GTGAGAATTC TCCACAGTCT CCTAAAGTGA CTATAACAGC TCCCAAAAAG 
AGGCCGCATA CGCCTTCTCA ATGCAAGGAA CGGAATTTAC AAGATCCTGT GCCAAGGGAA 
TCGCCAAAGT CTTGGTTTTT TGACAGCCGA TCAAAGTCTT TACCATCACC TCATCCAGTT 
CGCTACTTTG ATAACTCGGG TTTAGGCCTT TGCCCAGAAG AACAAATTGC TGGAGCAGAG 
AATGTGGATC CTCAGCCTGG TCTATGGCAT GCCTCCTCAG GAAAAGATGT GCCCGAGTGC 
AAGACCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965533.1
CDS	72..8063
Translation	MAEEGAVAVCVRVRPLNDREEALGGDTQIYWKTDNNAIYQVDGSKSFNFDRVFHSNETTKHVYEEIAVPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSQDYLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCDTQKMKPLIIREDFNRNVYVADLTEEVVYTSDMALKWITKGEKNRHYGITKMNQRSSRSHTIFRMILESREKGEPSNCDGSVKVSHLNLVDLAGSERAAQTGAEGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTVTPVSFDETLTTLQFASTAKYMKNTPYVNEVSSDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQTEKIQNLTRMLVTSSSLTSHQELKAKRKRRVTWCLGKINKMKDSNYINEFNMSTNITTRKAALTVVGEIDESLCSESDVFSNTFDTLNETEWNPATKLLSQENLESELNSLRANYDNLVLDYEQLRQEHEEMELKLKEKNDLDEFEALERKAEKDQEMQLIHEISNLKNLVKHAEVYNQDLENELSSKVELLREKEDQIKKLQKYIDSQKSEDIKTDLSYSSESTEDLKQMKQTLLDAETVVLDAKRESAFLRSENLELKEKMKELRSRYKQMENDIQLYQRQLEAKKKMQVDLEKELQSSFNEITKLTSLIDGKVPKGLLCNLELERKITDFQRELNEKVEENEALHKEVKLLSALKSLPSEIEMLRKEIHDKSEELYIITSEREKLNSEVVQKESRIQDLLEEIGKTKNDLATIRLNYESTDQEFQDFKNHHIELEQKYKMVLEENARMNQELGNLSEQAQKLGLSLDALSNTELSPKSEELLQKTTESQKRLNEVEELKEQLESTGSRLQTIEKEKTLIAEQLQQTLVEVRTLTQEKDDLKQLQESLQIERDQLKSDIQDTVNMNIDTQEQLRNALEALKQHQEVINTLRLKIAEKTSRHLETEENLGEAKDEFQEEMVDTDKKQNLKAKNTQALVVNVEDNELTEQQRNIFSLIQEKNELQRMLESVTAEKEQLKTDLRENIEMTIENQEELRILGDELKKQQEILVQEKNHTIKKEEELSRTYKKLAEVEEKLKEKNQQLQEKQLQLLSVQEEMSEMQKKMNEMENVNNEFRNRELTLERIEMERLEVAQKLHENDEEMKSLTKERNDLKELQESFELEREQLEGFIREIEVTIPVLHEEQELLPNVKEVSATQGTMNEGEILKKQSKTKDSMTPARIEIERLGLTAKLPESHEEIKSLTEERDNLKTIKEAVQVGQEQLKEDSREMLAEDSLNISEPQNKQEQSFDMKEREDEAEEIVSELEQLQEQLKAKEAPQPRVGMERLEMSEKLQEVLQSETNQLKENMREIIAKHLETEEELKVARCHLKEQEETIDKLRVNLSERETELSSLQKELEITNNELQKKIQELHEKQQFMSTKEITETQEKMSELEQLKEQLRVKDSSLQNIESEKLKLTEKLQESQDKIKIVIKERDELKRVQELLQMERDQLKANIKEIVAEEYQQTISTRNISKKTDQEANIERVIESSDAESQEKIQELQEKEHQLLKMKTVSETQEKICEMEHLKNQFEAQKSTLENIEMENARLTQRLHENLEEMRSVTKERDDLRSIEETLKVERDQLKENLRETVIRDLEKQEELRIARMHVKEHQETIDKLRGLVSEKTDELSNMQIDLENTNDALKTQIQELQEKEHQLLKVKNDLRENMYQTEQLKEQLQAQHSTLESVETERLRLSQKLHENLEEIKCVTEERDTLKRAERTLRMEQCQLRESLREKEAQDLEKQEELRIVCMHVKEHQETIDKLRGLVSEKTDEIANMQMDLENSNAKLQEKIQQLKANEHQLFKLKEDVSETQKKTSEIERLKKQLKGQSLTLDKIEMENLNLAQKLHENLEEMKSVMQERDNLKLERDQLKTNLQETIARDLETQQELKIALLHLKEHQETIDKFRERVSEKTTQISNIQKDLNKSKDELQKKDQQNQKEIVKYEKRLQCNEKHHLTESLREKCSRIKELLKRYSEMYNHYECLTRLSLDLEKEIETQKELSVTIKANLSLPHSQIKQIEKLLAVNQRCSMEFHRIIKRFQYVLSQITKIKEEQHESINKLEVVFIDEVEKHNELVIKIQHLQQDFNVPSGELTDLKLSQKMDLRIEEILKDLSENDFHLLKTEFQQVLSNRREITQFLEEWLNTHFDIEKLKNGIQKENDNICQVNNFYNNKIIAIMNESTEFEERYAIIAKEWERDLNSVKEKNEQLFKNYQTLKLTRTSGIPVNSATEDNKNLHVTSGATQLTTEKIQELEASLHEAKESALHKENKIVKMQKELEMTNDIIAKLQSQVNESNKCLEKTKERIQLLQDKVALGAKPYKEEIEDLKMKLVKMDLEKKKNAKEFEKEITSTKATVEHQKEVIRVLRENLRRNQQAQDTSIVSEYTDSQPPNKPLTCGGGSGIVQSTKALILKSGYIRLEKEISKLKQQNEQLIKQKNELLSNNHHLSNEVKTWKERILKREAHREVTCENSPQSPKVTITAPKKRPHTPSQCKERNLQDPVPRESPKSWFFDSRSKSLPSPHPVRYFDNSGLGLCPEEQIAGAENVDPQPGLWHASSGKDVPECKTQ

Target ORF information:

RefSeq Version	XM_014110058.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris centromere protein E, 312kDa (CENPE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110058.1

ORF Insert Sequence:

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGATTT GGAAACACAA 
CAAGAACTAA AAATTGCTCT TTTGCATTTG AAAGAACACC AAGAAACTAT TGATAAATTC 
AGAGAGAGAG TTTCAGAAAA GACAACTCAG ATTTCAAATA TCCAAAAGGA TTTAAATAAA 
TCTAAAGATG AGTTACAGAA AAAGGACCAA CAGAACCAAA AAGAGATAGT AAAATATGAA 
AAAAGGTTAC AATGTAATGA AAAACACCAC CTCACAGAAA GCCTAAGGGA GAAGTGCTCT 
AGGATAAAAG AGCTTCTGAA GAGATACTCA GAGATGTATA ATCATTATGA GTGCTTAACT 
AGACTATCCC TTGACTTGGA GAAAGAAATT GAAACCCAAA AAGAGCTTTC AGTTACAATA 
AAAGCAAACC TCTCACTTCC ACATTCACAA ATCAAACAAA TTGAAAAACT TCTCGCTGTA 
AACCAAAGAT GTTCAATGGA ATTCCACAGA ATCATCAAGA GATTTCAGTA CGTGTTAAGC 
CAGATTACAA AGATAAAGGA AGAACAACAT GAATCCATAA ATAAATTAGA AGTGGTTTTT 
ATTGATGAAG TGGAAAAGCA CAATGAACTG GTAATTAAAA TACAGCACCT TCAACAAGAT 
TTTAATGTAC CATCTGGAGA ATTAACGGAC CTCAAATTGA GCCAGAAGAT GGATCTACGT 
ATTGAGGAAA TTCTCAAAGA TCTTTCAGAA AATGATTTCC ACTTACTAAA GACTGAATTT 
CAACAAGTGC TAAGTAATAG GAGAGAAATA ACCCAGTTTT TGGAAGAATG GTTGAATACT 
CATTTTGATA TAGAAAAGCT TAAAAATGGC ATCCAGAAAG AAAATGATAA TATTTGTCAA 
GTGAATAACT TCTATAATAA CAAAATAATT GCTATAATGA ATGAATCTAC AGAGTTTGAG 
GAAAGATATG CCATCATAGC CAAAGAATGG GAACGTGACC TGAATTCAGT GAAAGAGAAA 
AATGAACAAC TATTTAAAAA CTATCAAACT TTGAAGCTCA CCCGGACGTC TGGTATCCCG 
GTAAATTCTG CCACAGAAGA CAATAAGAAT CTTCATGTTA CATCAGGAGC CACACAACTA 
ACCACAGAGA AAATTCAGGA GCTGGAAGCT TCACTGCATG AAGCTAAAGA AAGTGCTTTG 
CACAAGGAAA ACAAGATTGT AAAGATGCAG AAAGAACTTG AGATGACTAA TGACATCATA 
GCAAAACTTC AGTCGCAAGT TAATGAATCA AATAAATGCC TTGAAAAAAC AAAAGAAAGG 
ATCCAATTAC TTCAGGATAA AGTTGCTTTA GGAGCTAAGC CCTATAAAGA AGAAATTGAA 
GATCTCAAGA TGAAACTGGT GAAAATGGAT CTAGAAAAGA AGAAGAATGC CAAAGAGTTT 
GAAAAAGAAA TTACTTCTAC AAAGGCCACT GTAGAACATC AAAAAGAAGT TATAAGGGTA 
CTGAGAGAAA ATCTCAGAAG AAATCAACAA GCCCAGGATA CCTCAATTGT ATCAGAATAT 
ACGGACTCTC AGCCTCCAAA TAAACCCTTA ACTTGTGGAG GTGGCAGTGG CATCGTACAA 
AGCACAAAAG CTCTTATTTT AAAAAGTGGA TATATACGGC TAGAAAAGGA AATCTCTAAG 
TTAAAACAGC AAAATGAACA GCTAATAAAG CAAAAGAATG AACTATTAAG CAATAACCAT 
CATCTTTCCA ACGAAGTCAA AACATGGAAG GAGAGAATCC TTAAAAGAGA GGCTCACAGA 
GAAGTTACTT GTGAGAATTC TCCACAGTCT CCTAAAGTGA CTATAACAGC TCCCAAAAAG 
AGGCCGCATA CGCCTTCTCA ATGCAAGGAA CGGAATTTAC AAGATCCTGT GCCAAGGGAA 
TCGCCAAAGT CTTGGTTTTT TGACAGCCGA TCAAAGTCTT TACCATCACC TCATCCAGTT 
CGCTACTTTG ATAACTCGGG TTTAGGCCTT TGCCCAGAAG AACAAATTGC TGGAGCAGAG 
AATGTGGATC CTCAGCCTGG TCTATGGCAT GCCTCCTCAG GAAAAGATGT GCCCGAGTGC 
AAGACCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPE ( XM_014110058.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965533.1 Canis lupus familiaris centromere protein E (CENPE), transcript variant X3, mRNA.

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CloneID	OCe44623
Clone ID Related Accession (Same CDS sequence)	XM_014110058.1 , XM_014110058.2
Accession Version	XM_014110058.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	centromere-associated protein E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGATTT GGAAACACAA 
CAAGAACTAA AAATTGCTCT TTTGCATTTG AAAGAACACC AAGAAACTAT TGATAAATTC 
AGAGAGAGAG TTTCAGAAAA GACAACTCAG ATTTCAAATA TCCAAAAGGA TTTAAATAAA 
TCTAAAGATG AGTTACAGAA AAAGGACCAA CAGAACCAAA AAGAGATAGT AAAATATGAA 
AAAAGGTTAC AATGTAATGA AAAACACCAC CTCACAGAAA GCCTAAGGGA GAAGTGCTCT 
AGGATAAAAG AGCTTCTGAA GAGATACTCA GAGATGTATA ATCATTATGA GTGCTTAACT 
AGACTATCCC TTGACTTGGA GAAAGAAATT GAAACCCAAA AAGAGCTTTC AGTTACAATA 
AAAGCAAACC TCTCACTTCC ACATTCACAA ATCAAACAAA TTGAAAAACT TCTCGCTGTA 
AACCAAAGAT GTTCAATGGA ATTCCACAGA ATCATCAAGA GATTTCAGTA CGTGTTAAGC 
CAGATTACAA AGATAAAGGA AGAACAACAT GAATCCATAA ATAAATTAGA AGTGGTTTTT 
ATTGATGAAG TGGAAAAGCA CAATGAACTG GTAATTAAAA TACAGCACCT TCAACAAGAT 
TTTAATGTAC CATCTGGAGA ATTAACGGAC CTCAAATTGA GCCAGAAGAT GGATCTACGT 
ATTGAGGAAA TTCTCAAAGA TCTTTCAGAA AATGATTTCC ACTTACTAAA GACTGAATTT 
CAACAAGTGC TAAGTAATAG GAGAGAAATA ACCCAGTTTT TGGAAGAATG GTTGAATACT 
CATTTTGATA TAGAAAAGCT TAAAAATGGC ATCCAGAAAG AAAATGATAA TATTTGTCAA 
GTGAATAACT TCTATAATAA CAAAATAATT GCTATAATGA ATGAATCTAC AGAGTTTGAG 
GAAAGATATG CCATCATAGC CAAAGAATGG GAACGTGACC TGAATTCAGT GAAAGAGAAA 
AATGAACAAC TATTTAAAAA CTATCAAACT TTGAAGCTCA CCCGGACGTC TGGTATCCCG 
GTAAATTCTG CCACAGAAGA CAATAAGAAT CTTCATGTTA CATCAGGAGC CACACAACTA 
ACCACAGAGA AAATTCAGGA GCTGGAAGCT TCACTGCATG AAGCTAAAGA AAGTGCTTTG 
CACAAGGAAA ACAAGATTGT AAAGATGCAG AAAGAACTTG AGATGACTAA TGACATCATA 
GCAAAACTTC AGTCGCAAGT TAATGAATCA AATAAATGCC TTGAAAAAAC AAAAGAAAGG 
ATCCAATTAC TTCAGGATAA AGTTGCTTTA GGAGCTAAGC CCTATAAAGA AGAAATTGAA 
GATCTCAAGA TGAAACTGGT GAAAATGGAT CTAGAAAAGA AGAAGAATGC CAAAGAGTTT 
GAAAAAGAAA TTACTTCTAC AAAGGCCACT GTAGAACATC AAAAAGAAGT TATAAGGGTA 
CTGAGAGAAA ATCTCAGAAG AAATCAACAA GCCCAGGATA CCTCAATTGT ATCAGAATAT 
ACGGACTCTC AGCCTCCAAA TAAACCCTTA ACTTGTGGAG GTGGCAGTGG CATCGTACAA 
AGCACAAAAG CTCTTATTTT AAAAAGTGGA TATATACGGC TAGAAAAGGA AATCTCTAAG 
TTAAAACAGC AAAATGAACA GCTAATAAAG CAAAAGAATG AACTATTAAG CAATAACCAT 
CATCTTTCCA ACGAAGTCAA AACATGGAAG GAGAGAATCC TTAAAAGAGA GGCTCACAGA 
GAAGTTACTT GTGAGAATTC TCCACAGTCT CCTAAAGTGA CTATAACAGC TCCCAAAAAG 
AGGCCGCATA CGCCTTCTCA ATGCAAGGAA CGGAATTTAC AAGATCCTGT GCCAAGGGAA 
TCGCCAAAGT CTTGGTTTTT TGACAGCCGA TCAAAGTCTT TACCATCACC TCATCCAGTT 
CGCTACTTTG ATAACTCGGG TTTAGGCCTT TGCCCAGAAG AACAAATTGC TGGAGCAGAG 
AATGTGGATC CTCAGCCTGG TCTATGGCAT GCCTCCTCAG GAAAAGATGT GCCCGAGTGC 
AAGACCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965533.1
CDS	99..8090
Translation	MAEEGAVAVCVRVRPLNDREEALGGDTQIYWKTDNNAIYQVDGSKSFNFDRVFHSNETTKHVYEEIAVPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSQDYLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCDTQKMKPLIIREDFNRNVYVADLTEEVVYTSDMALKWITKGEKNRHYGITKMNQRSSRSHTIFRMILESREKGEPSNCDGSVKVSHLNLVDLAGSERAAQTGAEGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTVTPVSFDETLTTLQFASTAKYMKNTPYVNEVSSDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQTEKIQNLTRMLVTSSSLTSHQELKAKRKRRVTWCLGKINKMKDSNYINEFNMSTNITTRKAALTVVGEIDESLCSESDVFSNTFDTLNETEWNPATKLLSQENLESELNSLRANYDNLVLDYEQLRQEHEEMELKLKEKNDLDEFEALERKAEKDQEMQLIHEISNLKNLVKHAEVYNQDLENELSSKVELLREKEDQIKKLQKYIDSQKSEDIKTDLSYSSESTEDLKQMKQTLLDAETVVLDAKRESAFLRSENLELKEKMKELRSRYKQMENDIQLYQRQLEAKKKMQVDLEKELQSSFNEITKLTSLIDGKVPKGLLCNLELERKITDFQRELNEKVEENEALHKEVKLLSALKSLPSEIEMLRKEIHDKSEELYIITSEREKLNSEVVQKESRIQDLLEEIGKTKNDLATIRLNYESTDQEFQDFKNHHIELEQKYKMVLEENARMNQELGNLSEQAQKLGLSLDALSNTELSPKSEELLQKTTESQKRLNEVEELKEQLESTGSRLQTIEKEKTLIAEQLQQTLVEVRTLTQEKDDLKQLQESLQIERDQLKSDIQDTVNMNIDTQEQLRNALEALKQHQEVINTLRLKIAEKTSRHLETEENLGEAKDEFQEEMVDTDKKQNLKAKNTQALVVNVEDNELTEQQRNIFSLIQEKNELQRMLESVTAEKEQLKTDLRENIEMTIENQEELRILGDELKKQQEILVQEKNHTIKKEEELSRTYKKLAEVEEKLKEKNQQLQEKQLQLLSVQEEMSEMQKKMNEMENVNNEFRNRELTLERIEMERLEVAQKLHENDEEMKSLTKERNDLKELQESFELEREQLEGFIREIEVTIPVLHEEQELLPNVKEVSATQGTMNEGEILKKQSKTKDSMTPARIEIERLGLTAKLPESHEEIKSLTEERDNLKTIKEAVQVGQEQLKEDSREMLAEDSLNISEPQNKQEQSFDMKEREDEAEEIVSELEQLQEQLKAKEAPQPRVGMERLEMSEKLQEVLQSETNQLKENMREIIAKHLETEEELKVARCHLKEQEETIDKLRVNLSERETELSSLQKELEITNNELQKKIQELHEKQQFMSTKEITETQEKMSELEQLKEQLRVKDSSLQNIESEKLKLTEKLQESQDKIKIVIKERDELKRVQELLQMERDQLKANIKEIVAEEYQQTISTRNISKKTDQEANIERVIESSDAESQEKIQELQEKEHQLLKMKTVSETQEKICEMEHLKNQFEAQKSTLENIEMENARLTQRLHENLEEMRSVTKERDDLRSIEETLKVERDQLKENLRETVIRDLEKQEELRIARMHVKEHQETIDKLRGLVSEKTDELSNMQIDLENTNDALKTQIQELQEKEHQLLKVKNDLRENMYQTEQLKEQLQAQHSTLESVETERLRLSQKLHENLEEIKCVTEERDTLKRAERTLRMEQCQLRESLREKEAQDLEKQEELRIVCMHVKEHQETIDKLRGLVSEKTDEIANMQMDLENSNAKLQEKIQQLKANEHQLFKLKEDVSETQKKTSEIERLKKQLKGQSLTLDKIEMENLNLAQKLHENLEEMKSVMQERDNLKLERDQLKTNLQETIARDLETQQELKIALLHLKEHQETIDKFRERVSEKTTQISNIQKDLNKSKDELQKKDQQNQKEIVKYEKRLQCNEKHHLTESLREKCSRIKELLKRYSEMYNHYECLTRLSLDLEKEIETQKELSVTIKANLSLPHSQIKQIEKLLAVNQRCSMEFHRIIKRFQYVLSQITKIKEEQHESINKLEVVFIDEVEKHNELVIKIQHLQQDFNVPSGELTDLKLSQKMDLRIEEILKDLSENDFHLLKTEFQQVLSNRREITQFLEEWLNTHFDIEKLKNGIQKENDNICQVNNFYNNKIIAIMNESTEFEERYAIIAKEWERDLNSVKEKNEQLFKNYQTLKLTRTSGIPVNSATEDNKNLHVTSGATQLTTEKIQELEASLHEAKESALHKENKIVKMQKELEMTNDIIAKLQSQVNESNKCLEKTKERIQLLQDKVALGAKPYKEEIEDLKMKLVKMDLEKKKNAKEFEKEITSTKATVEHQKEVIRVLRENLRRNQQAQDTSIVSEYTDSQPPNKPLTCGGGSGIVQSTKALILKSGYIRLEKEISKLKQQNEQLIKQKNELLSNNHHLSNEVKTWKERILKREAHREVTCENSPQSPKVTITAPKKRPHTPSQCKERNLQDPVPRESPKSWFFDSRSKSLPSPHPVRYFDNSGLGLCPEEQIAGAENVDPQPGLWHASSGKDVPECKTQ

Target ORF information:

RefSeq Version	XM_014110058.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centromere protein E (CENPE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110058.2

ORF Insert Sequence:

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGATTT GGAAACACAA 
CAAGAACTAA AAATTGCTCT TTTGCATTTG AAAGAACACC AAGAAACTAT TGATAAATTC 
AGAGAGAGAG TTTCAGAAAA GACAACTCAG ATTTCAAATA TCCAAAAGGA TTTAAATAAA 
TCTAAAGATG AGTTACAGAA AAAGGACCAA CAGAACCAAA AAGAGATAGT AAAATATGAA 
AAAAGGTTAC AATGTAATGA AAAACACCAC CTCACAGAAA GCCTAAGGGA GAAGTGCTCT 
AGGATAAAAG AGCTTCTGAA GAGATACTCA GAGATGTATA ATCATTATGA GTGCTTAACT 
AGACTATCCC TTGACTTGGA GAAAGAAATT GAAACCCAAA AAGAGCTTTC AGTTACAATA 
AAAGCAAACC TCTCACTTCC ACATTCACAA ATCAAACAAA TTGAAAAACT TCTCGCTGTA 
AACCAAAGAT GTTCAATGGA ATTCCACAGA ATCATCAAGA GATTTCAGTA CGTGTTAAGC 
CAGATTACAA AGATAAAGGA AGAACAACAT GAATCCATAA ATAAATTAGA AGTGGTTTTT 
ATTGATGAAG TGGAAAAGCA CAATGAACTG GTAATTAAAA TACAGCACCT TCAACAAGAT 
TTTAATGTAC CATCTGGAGA ATTAACGGAC CTCAAATTGA GCCAGAAGAT GGATCTACGT 
ATTGAGGAAA TTCTCAAAGA TCTTTCAGAA AATGATTTCC ACTTACTAAA GACTGAATTT 
CAACAAGTGC TAAGTAATAG GAGAGAAATA ACCCAGTTTT TGGAAGAATG GTTGAATACT 
CATTTTGATA TAGAAAAGCT TAAAAATGGC ATCCAGAAAG AAAATGATAA TATTTGTCAA 
GTGAATAACT TCTATAATAA CAAAATAATT GCTATAATGA ATGAATCTAC AGAGTTTGAG 
GAAAGATATG CCATCATAGC CAAAGAATGG GAACGTGACC TGAATTCAGT GAAAGAGAAA 
AATGAACAAC TATTTAAAAA CTATCAAACT TTGAAGCTCA CCCGGACGTC TGGTATCCCG 
GTAAATTCTG CCACAGAAGA CAATAAGAAT CTTCATGTTA CATCAGGAGC CACACAACTA 
ACCACAGAGA AAATTCAGGA GCTGGAAGCT TCACTGCATG AAGCTAAAGA AAGTGCTTTG 
CACAAGGAAA ACAAGATTGT AAAGATGCAG AAAGAACTTG AGATGACTAA TGACATCATA 
GCAAAACTTC AGTCGCAAGT TAATGAATCA AATAAATGCC TTGAAAAAAC AAAAGAAAGG 
ATCCAATTAC TTCAGGATAA AGTTGCTTTA GGAGCTAAGC CCTATAAAGA AGAAATTGAA 
GATCTCAAGA TGAAACTGGT GAAAATGGAT CTAGAAAAGA AGAAGAATGC CAAAGAGTTT 
GAAAAAGAAA TTACTTCTAC AAAGGCCACT GTAGAACATC AAAAAGAAGT TATAAGGGTA 
CTGAGAGAAA ATCTCAGAAG AAATCAACAA GCCCAGGATA CCTCAATTGT ATCAGAATAT 
ACGGACTCTC AGCCTCCAAA TAAACCCTTA ACTTGTGGAG GTGGCAGTGG CATCGTACAA 
AGCACAAAAG CTCTTATTTT AAAAAGTGGA TATATACGGC TAGAAAAGGA AATCTCTAAG 
TTAAAACAGC AAAATGAACA GCTAATAAAG CAAAAGAATG AACTATTAAG CAATAACCAT 
CATCTTTCCA ACGAAGTCAA AACATGGAAG GAGAGAATCC TTAAAAGAGA GGCTCACAGA 
GAAGTTACTT GTGAGAATTC TCCACAGTCT CCTAAAGTGA CTATAACAGC TCCCAAAAAG 
AGGCCGCATA CGCCTTCTCA ATGCAAGGAA CGGAATTTAC AAGATCCTGT GCCAAGGGAA 
TCGCCAAAGT CTTGGTTTTT TGACAGCCGA TCAAAGTCTT TACCATCACC TCATCCAGTT 
CGCTACTTTG ATAACTCGGG TTTAGGCCTT TGCCCAGAAG AACAAATTGC TGGAGCAGAG 
AATGTGGATC CTCAGCCTGG TCTATGGCAT GCCTCCTCAG GAAAAGATGT GCCCGAGTGC 
AAGACCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPE ( XM_022413494.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269202.1 Canis lupus familiaris centromere protein E (CENPE), transcript variant X2, mRNA.

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CloneID	OCe284062
Clone ID Related Accession (Same CDS sequence)	XM_022413494.1
Accession Version	XM_022413494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	centromere-associated protein E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GAATGAACTA AGTTCAAAAG TAGAGCTGCT TAGAGAAAAG 
GAAGACCAGA TTAAGAAGCT ACAGAAATAC ATAGACTCTC AGAAGTCAGA AGATATTAAA 
ACGGACTTGT CCTACTCATC GGAAAGCACT GAAGACCTAA AACAAATGAA ACAGACTTTA 
CTTGATGCTG AAACTGTAGT CCTTGATGCT AAGAGAGAAT CAGCCTTTCT TAGAAGTGAA 
AATCTGGAGC TGAAAGAGAA AATGAAAGAA CTTAGAAGTA GATACAAGCA AATGGAGAAT 
GATATTCAGT TATATCAAAG ACAGTTGGAG GCAAAAAAGA AAATGCAAGT TGATCTGGAA 
AAAGAATTAC AATCTTCATT TAATGAGATA ACGAAACTCA CCTCCCTTAT TGATGGCAAA 
GTTCCAAAAG GTTTGCTCTG TAATTTGGAA CTGGAAAGAA AGATTACTGA TTTCCAGAGA 
GAACTAAATG AAAAAGTTGA AGAAAATGAA GCTTTGCATA AAGAAGTTAA GTTGCTCTCA 
GCGTTGAAAT CTTTACCCTC AGAAATAGAA ATGCTGAGGA AAGAGATACA TGATAAATCT 
GAAGAGCTCT ATATAATAAC ATCGGAAAGA GAGAAATTGA ATTCTGAAGT AGTTCAAAAG 
GAAAGTAGAA TTCAAGATTT ACTTGAAGAA ATTGGGAAAA CTAAAAATGA TCTAGCAACT 
ATCCGGTTGA ATTATGAAAG CACTGATCAA GAATTCCAAG ATTTTAAAAA TCATCATATT 
GAATTAGAGC AAAAGTATAA GATGGTCCTT GAAGAGAATG CAAGAATGAA TCAGGAATTA 
GGAAATCTCT CTGAACAAGC TCAAAAACTT GGTTTAAGTT TGGATGCTTT GAGCAACACA 
GAGCTTTCTC CCAAATCTGA AGAACTTCTA CAAAAAACAA CTGAGAGTCA AAAAAGATTA 
AATGAAGTGG AAGAGCTGAA GGAACAATTA GAAAGTACAG GTTCAAGGCT GCAAACTATA 
GAAAAGGAAA AAACACTGAT TGCTGAGCAA CTTCAGCAAA CCTTGGTAGA AGTAAGAACC 
TTAACCCAAG AAAAAGATGA CCTAAAACAA CTCCAGGAGA GCCTGCAGAT TGAGAGGGAC 
CAACTCAAAA GTGACATTCA GGATACTGTA AACATGAATA TAGACACCCA AGAACAATTA 
CGAAATGCTC TTGAAGCTTT GAAACAACAC CAAGAAGTAA TTAACACACT AAGACTGAAA 
ATTGCTGAGA AAACTTCCAG GCATTTGGAA ACAGAGGAAA ACCTAGGAGA AGCTAAGGAT 
GAATTCCAGG AAGAGATGGT TGACACAGAT AAAAAACAGA ATTTGAAAGC CAAAAATACT 
CAAGCACTGG TTGTGAATGT TGAAGATAAT GAGCTAACTG AGCAACAGAG AAATATATTT 
TCTTTAATAC AGGAGAAGAA TGAACTGCAA CGAATGTTAG AGAGTGTTAC AGCAGAAAAG 
GAACAGTTAA AGACTGACTT AAGGGAAAAT ATTGAAATGA CTATTGAAAA CCAAGAAGAA 
TTGAGAATTC TTGGAGATGA ACTTAAAAAG CAACAAGAGA TACTTGTACA AGAAAAGAAC 
CACACTATAA AGAAAGAAGA AGAACTCTCT AGGACCTATA AGAAATTAGC AGAAGTTGAA 
GAAAAGTTAA AGGAAAAGAA CCAACAACTA CAAGAAAAAC AGCTACAACT TCTTAGTGTA 
CAAGAAGAAA TGAGTGAGAT GCAGAAAAAG ATGAATGAAA TGGAGAATGT AAACAATGAA 
TTTAGGAACC GAGAACTGAC ATTGGAACGT ATAGAAATGG AGAGGCTAGA GGTGGCTCAG 
AAGCTCCATG AAAATGATGA GGAAATGAAA TCATTAACCA AAGAAAGAAA TGATCTAAAG 
GAATTACAGG AATCATTTGA ATTAGAGAGA GAGCAACTTG AAGGATTCAT AAGAGAAATC 
GAAGTTACAA TCCCAGTGCT TCATGAGGAA CAGGAGCTAC TGCCTAATGT GAAAGAAGTC 
AGTGCTACTC AGGGAACAAT GAATGAAGGG GAGATATTGA AAAAGCAGTC CAAAACCAAG 
GATTCAATGA CACCAGCACG CATAGAAATA GAAAGGCTTG GGTTGACTGC AAAACTTCCA 
GAAAGTCATG AAGAAATAAA ATCTCTAACT GAGGAAAGAG ACAACCTTAA AACAATAAAA 
GAAGCAGTTC AGGTTGGACA AGAGCAACTG AAAGAAGACA GTAGAGAAAT GTTGGCTGAA 
GATTCTCTTA ATATCTCAGA ACCTCAAAAT AAACAAGAAC AGTCCTTTGA CATGAAAGAA 
AGAGAAGATG AAGCTGAGGA AATCGTGAGT GAGTTGGAGC AACTGCAGGA GCAGCTTAAA 
GCCAAAGAGG CACCACAGCC AAGAGTGGGC ATGGAAAGGC TTGAGATGTC TGAGAAGCTA 
CAAGAAGTTC TTCAGTCTGA AACTAACCAA CTCAAAGAAA ACATGAGAGA AATCATAGCT 
AAACACCTGG AAACTGAAGA GGAACTGAAA GTTGCTCGTT GTCACCTGAA AGAACAAGAG 
GAAACTATTG ATAAGCTGAG AGTGAATCTT TCAGAGAGGG AAACTGAACT ATCCAGCCTT 
CAAAAGGAAT TAGAAATAAC CAATAATGAA TTACAGAAAA AGATCCAAGA GCTTCATGAG 
AAACAGCAAT TTATGAGCAC CAAAGAAATC ACTGAGACTC AGGAAAAAAT GAGTGAACTG 
GAGCAATTAA AGGAGCAACT CAGAGTCAAA GACTCATCAT TACAAAATAT AGAAAGTGAG 
AAGCTCAAGT TGACTGAGAA ACTTCAGGAA AGTCAAGATA AAATAAAAAT TGTAATTAAG 
GAAAGAGATG AACTGAAAAG AGTACAGGAA CTTCTTCAAA TGGAGAGAGA CCAACTCAAA 
GCAAACATTA AAGAAATTGT AGCTGAGGAG TACCAACAAA CCATTAGTAC TAGGAATATT 
TCAAAGAAGA CAGACCAAGA AGCAAATATT GAGAGAGTTA TAGAAAGTTC AGATGCTGAA 
TCACAAGAAA AGATTCAAGA ACTTCAGGAG AAAGAGCATC AGCTTCTTAA GATGAAAACT 
GTCAGTGAGA CTCAGGAAAA AATATGTGAG ATGGAACACT TGAAGAACCA GTTTGAGGCC 
CAAAAGTCAA CTCTGGAAAA TATAGAAATG GAGAATGCAA GGTTAACTCA GAGACTTCAT 
GAAAACCTTG AAGAAATGAG ATCTGTTACA AAAGAAAGAG ATGATCTTAG GAGTATAGAG 
GAGACTCTCA AAGTAGAGAG AGACCAGCTC AAGGAAAACC TGAGAGAAAC TGTAATTAGA 
GACCTGGAAA AACAAGAGGA ACTAAGAATT GCTCGCATGC ATGTGAAAGA GCACCAGGAA 
ACTATTGATA AACTCAGGGG CCTTGTTTCT GAGAAGACAG ATGAACTATC AAATATGCAG 
ATAGATTTAG AAAACACAAA TGATGCCTTA AAAACACAGA TCCAAGAACT TCAGGAGAAA 
GAACATCAAC TTCTTAAAGT AAAAAATGAT CTCAGAGAAA ATATGTATCA AACAGAGCAA 
TTGAAGGAGC AATTACAGGC CCAACATTCA ACCCTGGAAA GTGTAGAAAC TGAGAGGCTG 
AGGTTAAGTC AGAAACTTCA TGAAAACCTT GAAGAAATAA AATGTGTTAC TGAGGAAAGA 
GACACTCTAA AAAGAGCAGA GAGAACCCTC AGGATGGAGC AATGCCAGCT CAGAGAAAGC 
CTCAGAGAAA AGGAAGCTCA AGATCTGGAA AAACAAGAAG AACTAAGAAT TGTTTGCATG 
CATGTGAAAG AGCACCAGGA AACTATTGAT AAACTCAGAG GCCTTGTTTC TGAGAAGACA 
GATGAAATAG CAAATATGCA AATGGATTTA GAAAATTCAA ATGCTAAATT ACAAGAAAAG 
ATTCAACAAC TTAAGGCAAA TGAACATCAA CTTTTTAAGC TAAAGGAGGA TGTCAGTGAG 
ACACAGAAAA AGACGTCTGA AATAGAGCGA TTGAAGAAAC AATTGAAGGG CCAAAGTTTA 
ACTCTGGATA AAATAGAAAT GGAGAACTTA AATTTGGCTC AGAAACTTCA TGAAAACCTT 
GAAGAAATGA AATCTGTAAT GCAAGAAAGA GATAATCTGA AATTGGAGAG AGACCAGCTC 
AAGACAAACC TACAGGAAAC CATAGCCAGA GATTTGGAAA CACAACAAGA ACTAAAAATT 
GCTCTTTTGC ATTTGAAAGA ACACCAAGAA ACTATTGATA AATTCAGAGA GAGAGTTTCA 
GAAAAGACAA CTCAGATTTC AAATATCCAA AAGGATTTAA ATAAATCTAA AGATGAGTTA 
CAGAAAAAGA TAGAAGAACT TCAGAAAAAA GAGCTTCAAC TTCTCAAAAT GAAAGAAGAT 
GTCAATAAAA CTCATAAAAA TATAAACAAA ATGGAACAAT TGAAGCAACA GTTTGAGGCC 
CAGAACTTAT CTATGCAAAG TGTGGAAATG GATAACTTAG ACTTGACAAA GAAACTTCAT 
AAATGCCTTG AAGAAAAAAG AATTATAGCT AAGGAAAGAG ATGAGCTAAG GAGGATAAAA 
GAGTCTCTCA AAATGGAAAG AGACCAATTC AGAGAAACCT TAACAGAGAT GATAGCTAGC 
GACCAACAGA ACCAAAAAGA GATAGTAAAA TATGAAAAAA GGTTACAATG TAATGAAAAA 
CACCACCTCA CAGAAAGCCT AAGGGAGAAG TGCTCTAGGA TAAAAGAGCT TCTGAAGAGA 
TACTCAGAGA TGTATAATCA TTATGAGTGC TTAACTAGAC TATCCCTTGA CTTGGAGAAA 
GAAATTGAAA CCCAAAAAGA GCTTTCAGTT ACAATAAAAG CAAACCTCTC ACTTCCACAT 
TCACAAATCA AACAAATTGA AAAACTTCTC GCTGTAAACC AAAGATGTTC AATGGAATTC 
CACAGAATCA TCAAGAGATT TCAGTACGTG TTAAGCCAGA TTACAAAGAT AAAGGAAGAA 
CAACATGAAT CCATAAATAA ATTAGAAGTG GTTTTTATTG ATGAAGTGGA AAAGCACAAT 
GAACTGGTAA TTAAAATACA GCACCTTCAA CAAGATTTTA ATGTACCATC TGGAGAATTA 
ACGGACCTCA AATTGAGCCA GAAGATGGAT CTACGTATTG AGGAAATTCT CAAAGATCTT 
TCAGAAAATG ATTTCCACTT ACTAAAGACT GAATTTCAAC AAGTGCTAAG TAATAGGAGA 
GAAATAACCC AGTTTTTGGA AGAATGGTTG AATACTCATT TTGATATAGA AAAGCTTAAA 
AATGGCATCC AGAAAGAAAA TGATAATATT TGTCAAGTGA ATAACTTCTA TAATAACAAA 
ATAATTGCTA TAATGAATGA ATCTACAGAG TTTGAGGAAA GATATGCCAT CATAGCCAAA 
GAATGGGAAC GTGACCTGAA TTCAGTGAAA GAGAAAAATG AACAACTATT TAAAAACTAT 
CAAACTTTGA AGCTCACCCG GACGTCTGGT ATCCCGGTAA ATTCTGCCAC AGAAGACAAT 
AAGAATCTTC ATGTTACATC AGGAGCCACA CAACTAACCA CAGAGAAAAT TCAGGAGCTG 
GAAGCTTCAC TGCATGAAGC TAAAGAAAGT GCTTTGCACA AGGAAAACAA GATTGTAAAG 
ATGCAGAAAG AACTTGAGAT GACTAATGAC ATCATAGCAA AACTTCAGTC GCAAGTTAAT 
GAATCAAATA AATGCCTTGA AAAAACAAAA GAAAGGATCC AATTACTTCA GGATAAAGTT 
GCTTTAGGAG CTAAGCCCTA TAAAGAAGAA ATTGAAGATC TCAAGATGAA ACTGGTGAAA 
ATGGATCTAG AAAAGAAGAA GAATGCCAAA GAGTTTGAAA AAGAAATTAC TTCTACAAAG 
GCCACTGTAG AACATCAAAA AGAAGTTATA AGGGTACTGA GAGAAAATCT CAGAAGAAAT 
CAACAAGCCC AGGATACCTC AATTGTATCA GAATATACGG ACTCTCAGCC TCCAAATAAA 
CCCTTAACTT GTGGAGGTGG CAGTGGCATC GTACAAAGCA CAAAAGCTCT TATTTTAAAA 
AGTGGATATA TACGGCTAGA AAAGGAAATC TCTAAGTTAA AACAGCAAAA TGAACAGCTA 
ATAAAGCAAA AGAATGAACT ATTAAGCAAT AACCATCATC TTTCCAACGA AGTCAAAACA 
TGGAAGGAGA GAATCCTTAA AAGAGAGGCT CACAGAGAAG TTACTTGTGA GAATTCTCCA 
CAGTCTCCTA AAGTGACTAT AACAGCTCCC AAAAAGAGGC CGCATACGCC TTCTCAATGC 
AAGGAACGGA ATTTACAAGA TCCTGTGCCA AGGGAATCGC CAAAGTCTTG GTTTTTTGAC 
AGCCGATCAA AGTCTTTACC ATCACCTCAT CCAGTTCGCT ACTTTGATAA CTCGGGTTTA 
GGCCTTTGCC CAGAAGAACA AATTGCTGGA GCAGAGAATG TGGATCCTCA GCCTGGTCTA 
TGGCATGCCT CCTCAGGAAA AGATGTGCCC GAGTGCAAGA CCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269202.1
CDS	99..8306
Translation	MAEEGAVAVCVRVRPLNDREEALGGDTQIYWKTDNNAIYQVDGSKSFNFDRVFHSNETTKHVYEEIAVPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSQDYLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCDTQKMKPLIIREDFNRNVYVADLTEEVVYTSDMALKWITKGEKNRHYGITKMNQRSSRSHTIFRMILESREKGEPSNCDGSVKVSHLNLVDLAGSERAAQTGAEGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTVTPVSFDETLTTLQFASTAKYMKNTPYVNEVSSDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQTEKIQNLTRMLVTSSSLTSHQELKAKRKRRVTWCLGKINKMKDSNYINEFNMSTNITTRKAALTVVGEIDESLCSESDVFSNTFDTLNETEWNPATKLLSQENLESELNSLRANYDNLVLDYEQLRQEHEEMELKLKEKNDLDEFEALERKAEKDQENELSSKVELLREKEDQIKKLQKYIDSQKSEDIKTDLSYSSESTEDLKQMKQTLLDAETVVLDAKRESAFLRSENLELKEKMKELRSRYKQMENDIQLYQRQLEAKKKMQVDLEKELQSSFNEITKLTSLIDGKVPKGLLCNLELERKITDFQRELNEKVEENEALHKEVKLLSALKSLPSEIEMLRKEIHDKSEELYIITSEREKLNSEVVQKESRIQDLLEEIGKTKNDLATIRLNYESTDQEFQDFKNHHIELEQKYKMVLEENARMNQELGNLSEQAQKLGLSLDALSNTELSPKSEELLQKTTESQKRLNEVEELKEQLESTGSRLQTIEKEKTLIAEQLQQTLVEVRTLTQEKDDLKQLQESLQIERDQLKSDIQDTVNMNIDTQEQLRNALEALKQHQEVINTLRLKIAEKTSRHLETEENLGEAKDEFQEEMVDTDKKQNLKAKNTQALVVNVEDNELTEQQRNIFSLIQEKNELQRMLESVTAEKEQLKTDLRENIEMTIENQEELRILGDELKKQQEILVQEKNHTIKKEEELSRTYKKLAEVEEKLKEKNQQLQEKQLQLLSVQEEMSEMQKKMNEMENVNNEFRNRELTLERIEMERLEVAQKLHENDEEMKSLTKERNDLKELQESFELEREQLEGFIREIEVTIPVLHEEQELLPNVKEVSATQGTMNEGEILKKQSKTKDSMTPARIEIERLGLTAKLPESHEEIKSLTEERDNLKTIKEAVQVGQEQLKEDSREMLAEDSLNISEPQNKQEQSFDMKEREDEAEEIVSELEQLQEQLKAKEAPQPRVGMERLEMSEKLQEVLQSETNQLKENMREIIAKHLETEEELKVARCHLKEQEETIDKLRVNLSERETELSSLQKELEITNNELQKKIQELHEKQQFMSTKEITETQEKMSELEQLKEQLRVKDSSLQNIESEKLKLTEKLQESQDKIKIVIKERDELKRVQELLQMERDQLKANIKEIVAEEYQQTISTRNISKKTDQEANIERVIESSDAESQEKIQELQEKEHQLLKMKTVSETQEKICEMEHLKNQFEAQKSTLENIEMENARLTQRLHENLEEMRSVTKERDDLRSIEETLKVERDQLKENLRETVIRDLEKQEELRIARMHVKEHQETIDKLRGLVSEKTDELSNMQIDLENTNDALKTQIQELQEKEHQLLKVKNDLRENMYQTEQLKEQLQAQHSTLESVETERLRLSQKLHENLEEIKCVTEERDTLKRAERTLRMEQCQLRESLREKEAQDLEKQEELRIVCMHVKEHQETIDKLRGLVSEKTDEIANMQMDLENSNAKLQEKIQQLKANEHQLFKLKEDVSETQKKTSEIERLKKQLKGQSLTLDKIEMENLNLAQKLHENLEEMKSVMQERDNLKLERDQLKTNLQETIARDLETQQELKIALLHLKEHQETIDKFRERVSEKTTQISNIQKDLNKSKDELQKKIEELQKKELQLLKMKEDVNKTHKNINKMEQLKQQFEAQNLSMQSVEMDNLDLTKKLHKCLEEKRIIAKERDELRRIKESLKMERDQFRETLTEMIASDQQNQKEIVKYEKRLQCNEKHHLTESLREKCSRIKELLKRYSEMYNHYECLTRLSLDLEKEIETQKELSVTIKANLSLPHSQIKQIEKLLAVNQRCSMEFHRIIKRFQYVLSQITKIKEEQHESINKLEVVFIDEVEKHNELVIKIQHLQQDFNVPSGELTDLKLSQKMDLRIEEILKDLSENDFHLLKTEFQQVLSNRREITQFLEEWLNTHFDIEKLKNGIQKENDNICQVNNFYNNKIIAIMNESTEFEERYAIIAKEWERDLNSVKEKNEQLFKNYQTLKLTRTSGIPVNSATEDNKNLHVTSGATQLTTEKIQELEASLHEAKESALHKENKIVKMQKELEMTNDIIAKLQSQVNESNKCLEKTKERIQLLQDKVALGAKPYKEEIEDLKMKLVKMDLEKKKNAKEFEKEITSTKATVEHQKEVIRVLRENLRRNQQAQDTSIVSEYTDSQPPNKPLTCGGGSGIVQSTKALILKSGYIRLEKEISKLKQQNEQLIKQKNELLSNNHHLSNEVKTWKERILKREAHREVTCENSPQSPKVTITAPKKRPHTPSQCKERNLQDPVPRESPKSWFFDSRSKSLPSPHPVRYFDNSGLGLCPEEQIAGAENVDPQPGLWHASSGKDVPECKTQ

Target ORF information:

RefSeq Version	XM_022413494.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centromere protein E (CENPE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413494.1

ORF Insert Sequence:

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GAATGAACTA AGTTCAAAAG TAGAGCTGCT TAGAGAAAAG 
GAAGACCAGA TTAAGAAGCT ACAGAAATAC ATAGACTCTC AGAAGTCAGA AGATATTAAA 
ACGGACTTGT CCTACTCATC GGAAAGCACT GAAGACCTAA AACAAATGAA ACAGACTTTA 
CTTGATGCTG AAACTGTAGT CCTTGATGCT AAGAGAGAAT CAGCCTTTCT TAGAAGTGAA 
AATCTGGAGC TGAAAGAGAA AATGAAAGAA CTTAGAAGTA GATACAAGCA AATGGAGAAT 
GATATTCAGT TATATCAAAG ACAGTTGGAG GCAAAAAAGA AAATGCAAGT TGATCTGGAA 
AAAGAATTAC AATCTTCATT TAATGAGATA ACGAAACTCA CCTCCCTTAT TGATGGCAAA 
GTTCCAAAAG GTTTGCTCTG TAATTTGGAA CTGGAAAGAA AGATTACTGA TTTCCAGAGA 
GAACTAAATG AAAAAGTTGA AGAAAATGAA GCTTTGCATA AAGAAGTTAA GTTGCTCTCA 
GCGTTGAAAT CTTTACCCTC AGAAATAGAA ATGCTGAGGA AAGAGATACA TGATAAATCT 
GAAGAGCTCT ATATAATAAC ATCGGAAAGA GAGAAATTGA ATTCTGAAGT AGTTCAAAAG 
GAAAGTAGAA TTCAAGATTT ACTTGAAGAA ATTGGGAAAA CTAAAAATGA TCTAGCAACT 
ATCCGGTTGA ATTATGAAAG CACTGATCAA GAATTCCAAG ATTTTAAAAA TCATCATATT 
GAATTAGAGC AAAAGTATAA GATGGTCCTT GAAGAGAATG CAAGAATGAA TCAGGAATTA 
GGAAATCTCT CTGAACAAGC TCAAAAACTT GGTTTAAGTT TGGATGCTTT GAGCAACACA 
GAGCTTTCTC CCAAATCTGA AGAACTTCTA CAAAAAACAA CTGAGAGTCA AAAAAGATTA 
AATGAAGTGG AAGAGCTGAA GGAACAATTA GAAAGTACAG GTTCAAGGCT GCAAACTATA 
GAAAAGGAAA AAACACTGAT TGCTGAGCAA CTTCAGCAAA CCTTGGTAGA AGTAAGAACC 
TTAACCCAAG AAAAAGATGA CCTAAAACAA CTCCAGGAGA GCCTGCAGAT TGAGAGGGAC 
CAACTCAAAA GTGACATTCA GGATACTGTA AACATGAATA TAGACACCCA AGAACAATTA 
CGAAATGCTC TTGAAGCTTT GAAACAACAC CAAGAAGTAA TTAACACACT AAGACTGAAA 
ATTGCTGAGA AAACTTCCAG GCATTTGGAA ACAGAGGAAA ACCTAGGAGA AGCTAAGGAT 
GAATTCCAGG AAGAGATGGT TGACACAGAT AAAAAACAGA ATTTGAAAGC CAAAAATACT 
CAAGCACTGG TTGTGAATGT TGAAGATAAT GAGCTAACTG AGCAACAGAG AAATATATTT 
TCTTTAATAC AGGAGAAGAA TGAACTGCAA CGAATGTTAG AGAGTGTTAC AGCAGAAAAG 
GAACAGTTAA AGACTGACTT AAGGGAAAAT ATTGAAATGA CTATTGAAAA CCAAGAAGAA 
TTGAGAATTC TTGGAGATGA ACTTAAAAAG CAACAAGAGA TACTTGTACA AGAAAAGAAC 
CACACTATAA AGAAAGAAGA AGAACTCTCT AGGACCTATA AGAAATTAGC AGAAGTTGAA 
GAAAAGTTAA AGGAAAAGAA CCAACAACTA CAAGAAAAAC AGCTACAACT TCTTAGTGTA 
CAAGAAGAAA TGAGTGAGAT GCAGAAAAAG ATGAATGAAA TGGAGAATGT AAACAATGAA 
TTTAGGAACC GAGAACTGAC ATTGGAACGT ATAGAAATGG AGAGGCTAGA GGTGGCTCAG 
AAGCTCCATG AAAATGATGA GGAAATGAAA TCATTAACCA AAGAAAGAAA TGATCTAAAG 
GAATTACAGG AATCATTTGA ATTAGAGAGA GAGCAACTTG AAGGATTCAT AAGAGAAATC 
GAAGTTACAA TCCCAGTGCT TCATGAGGAA CAGGAGCTAC TGCCTAATGT GAAAGAAGTC 
AGTGCTACTC AGGGAACAAT GAATGAAGGG GAGATATTGA AAAAGCAGTC CAAAACCAAG 
GATTCAATGA CACCAGCACG CATAGAAATA GAAAGGCTTG GGTTGACTGC AAAACTTCCA 
GAAAGTCATG AAGAAATAAA ATCTCTAACT GAGGAAAGAG ACAACCTTAA AACAATAAAA 
GAAGCAGTTC AGGTTGGACA AGAGCAACTG AAAGAAGACA GTAGAGAAAT GTTGGCTGAA 
GATTCTCTTA ATATCTCAGA ACCTCAAAAT AAACAAGAAC AGTCCTTTGA CATGAAAGAA 
AGAGAAGATG AAGCTGAGGA AATCGTGAGT GAGTTGGAGC AACTGCAGGA GCAGCTTAAA 
GCCAAAGAGG CACCACAGCC AAGAGTGGGC ATGGAAAGGC TTGAGATGTC TGAGAAGCTA 
CAAGAAGTTC TTCAGTCTGA AACTAACCAA CTCAAAGAAA ACATGAGAGA AATCATAGCT 
AAACACCTGG AAACTGAAGA GGAACTGAAA GTTGCTCGTT GTCACCTGAA AGAACAAGAG 
GAAACTATTG ATAAGCTGAG AGTGAATCTT TCAGAGAGGG AAACTGAACT ATCCAGCCTT 
CAAAAGGAAT TAGAAATAAC CAATAATGAA TTACAGAAAA AGATCCAAGA GCTTCATGAG 
AAACAGCAAT TTATGAGCAC CAAAGAAATC ACTGAGACTC AGGAAAAAAT GAGTGAACTG 
GAGCAATTAA AGGAGCAACT CAGAGTCAAA GACTCATCAT TACAAAATAT AGAAAGTGAG 
AAGCTCAAGT TGACTGAGAA ACTTCAGGAA AGTCAAGATA AAATAAAAAT TGTAATTAAG 
GAAAGAGATG AACTGAAAAG AGTACAGGAA CTTCTTCAAA TGGAGAGAGA CCAACTCAAA 
GCAAACATTA AAGAAATTGT AGCTGAGGAG TACCAACAAA CCATTAGTAC TAGGAATATT 
TCAAAGAAGA CAGACCAAGA AGCAAATATT GAGAGAGTTA TAGAAAGTTC AGATGCTGAA 
TCACAAGAAA AGATTCAAGA ACTTCAGGAG AAAGAGCATC AGCTTCTTAA GATGAAAACT 
GTCAGTGAGA CTCAGGAAAA AATATGTGAG ATGGAACACT TGAAGAACCA GTTTGAGGCC 
CAAAAGTCAA CTCTGGAAAA TATAGAAATG GAGAATGCAA GGTTAACTCA GAGACTTCAT 
GAAAACCTTG AAGAAATGAG ATCTGTTACA AAAGAAAGAG ATGATCTTAG GAGTATAGAG 
GAGACTCTCA AAGTAGAGAG AGACCAGCTC AAGGAAAACC TGAGAGAAAC TGTAATTAGA 
GACCTGGAAA AACAAGAGGA ACTAAGAATT GCTCGCATGC ATGTGAAAGA GCACCAGGAA 
ACTATTGATA AACTCAGGGG CCTTGTTTCT GAGAAGACAG ATGAACTATC AAATATGCAG 
ATAGATTTAG AAAACACAAA TGATGCCTTA AAAACACAGA TCCAAGAACT TCAGGAGAAA 
GAACATCAAC TTCTTAAAGT AAAAAATGAT CTCAGAGAAA ATATGTATCA AACAGAGCAA 
TTGAAGGAGC AATTACAGGC CCAACATTCA ACCCTGGAAA GTGTAGAAAC TGAGAGGCTG 
AGGTTAAGTC AGAAACTTCA TGAAAACCTT GAAGAAATAA AATGTGTTAC TGAGGAAAGA 
GACACTCTAA AAAGAGCAGA GAGAACCCTC AGGATGGAGC AATGCCAGCT CAGAGAAAGC 
CTCAGAGAAA AGGAAGCTCA AGATCTGGAA AAACAAGAAG AACTAAGAAT TGTTTGCATG 
CATGTGAAAG AGCACCAGGA AACTATTGAT AAACTCAGAG GCCTTGTTTC TGAGAAGACA 
GATGAAATAG CAAATATGCA AATGGATTTA GAAAATTCAA ATGCTAAATT ACAAGAAAAG 
ATTCAACAAC TTAAGGCAAA TGAACATCAA CTTTTTAAGC TAAAGGAGGA TGTCAGTGAG 
ACACAGAAAA AGACGTCTGA AATAGAGCGA TTGAAGAAAC AATTGAAGGG CCAAAGTTTA 
ACTCTGGATA AAATAGAAAT GGAGAACTTA AATTTGGCTC AGAAACTTCA TGAAAACCTT 
GAAGAAATGA AATCTGTAAT GCAAGAAAGA GATAATCTGA AATTGGAGAG AGACCAGCTC 
AAGACAAACC TACAGGAAAC CATAGCCAGA GATTTGGAAA CACAACAAGA ACTAAAAATT 
GCTCTTTTGC ATTTGAAAGA ACACCAAGAA ACTATTGATA AATTCAGAGA GAGAGTTTCA 
GAAAAGACAA CTCAGATTTC AAATATCCAA AAGGATTTAA ATAAATCTAA AGATGAGTTA 
CAGAAAAAGA TAGAAGAACT TCAGAAAAAA GAGCTTCAAC TTCTCAAAAT GAAAGAAGAT 
GTCAATAAAA CTCATAAAAA TATAAACAAA ATGGAACAAT TGAAGCAACA GTTTGAGGCC 
CAGAACTTAT CTATGCAAAG TGTGGAAATG GATAACTTAG ACTTGACAAA GAAACTTCAT 
AAATGCCTTG AAGAAAAAAG AATTATAGCT AAGGAAAGAG ATGAGCTAAG GAGGATAAAA 
GAGTCTCTCA AAATGGAAAG AGACCAATTC AGAGAAACCT TAACAGAGAT GATAGCTAGC 
GACCAACAGA ACCAAAAAGA GATAGTAAAA TATGAAAAAA GGTTACAATG TAATGAAAAA 
CACCACCTCA CAGAAAGCCT AAGGGAGAAG TGCTCTAGGA TAAAAGAGCT TCTGAAGAGA 
TACTCAGAGA TGTATAATCA TTATGAGTGC TTAACTAGAC TATCCCTTGA CTTGGAGAAA 
GAAATTGAAA CCCAAAAAGA GCTTTCAGTT ACAATAAAAG CAAACCTCTC ACTTCCACAT 
TCACAAATCA AACAAATTGA AAAACTTCTC GCTGTAAACC AAAGATGTTC AATGGAATTC 
CACAGAATCA TCAAGAGATT TCAGTACGTG TTAAGCCAGA TTACAAAGAT AAAGGAAGAA 
CAACATGAAT CCATAAATAA ATTAGAAGTG GTTTTTATTG ATGAAGTGGA AAAGCACAAT 
GAACTGGTAA TTAAAATACA GCACCTTCAA CAAGATTTTA ATGTACCATC TGGAGAATTA 
ACGGACCTCA AATTGAGCCA GAAGATGGAT CTACGTATTG AGGAAATTCT CAAAGATCTT 
TCAGAAAATG ATTTCCACTT ACTAAAGACT GAATTTCAAC AAGTGCTAAG TAATAGGAGA 
GAAATAACCC AGTTTTTGGA AGAATGGTTG AATACTCATT TTGATATAGA AAAGCTTAAA 
AATGGCATCC AGAAAGAAAA TGATAATATT TGTCAAGTGA ATAACTTCTA TAATAACAAA 
ATAATTGCTA TAATGAATGA ATCTACAGAG TTTGAGGAAA GATATGCCAT CATAGCCAAA 
GAATGGGAAC GTGACCTGAA TTCAGTGAAA GAGAAAAATG AACAACTATT TAAAAACTAT 
CAAACTTTGA AGCTCACCCG GACGTCTGGT ATCCCGGTAA ATTCTGCCAC AGAAGACAAT 
AAGAATCTTC ATGTTACATC AGGAGCCACA CAACTAACCA CAGAGAAAAT TCAGGAGCTG 
GAAGCTTCAC TGCATGAAGC TAAAGAAAGT GCTTTGCACA AGGAAAACAA GATTGTAAAG 
ATGCAGAAAG AACTTGAGAT GACTAATGAC ATCATAGCAA AACTTCAGTC GCAAGTTAAT 
GAATCAAATA AATGCCTTGA AAAAACAAAA GAAAGGATCC AATTACTTCA GGATAAAGTT 
GCTTTAGGAG CTAAGCCCTA TAAAGAAGAA ATTGAAGATC TCAAGATGAA ACTGGTGAAA 
ATGGATCTAG AAAAGAAGAA GAATGCCAAA GAGTTTGAAA AAGAAATTAC TTCTACAAAG 
GCCACTGTAG AACATCAAAA AGAAGTTATA AGGGTACTGA GAGAAAATCT CAGAAGAAAT 
CAACAAGCCC AGGATACCTC AATTGTATCA GAATATACGG ACTCTCAGCC TCCAAATAAA 
CCCTTAACTT GTGGAGGTGG CAGTGGCATC GTACAAAGCA CAAAAGCTCT TATTTTAAAA 
AGTGGATATA TACGGCTAGA AAAGGAAATC TCTAAGTTAA AACAGCAAAA TGAACAGCTA 
ATAAAGCAAA AGAATGAACT ATTAAGCAAT AACCATCATC TTTCCAACGA AGTCAAAACA 
TGGAAGGAGA GAATCCTTAA AAGAGAGGCT CACAGAGAAG TTACTTGTGA GAATTCTCCA 
CAGTCTCCTA AAGTGACTAT AACAGCTCCC AAAAAGAGGC CGCATACGCC TTCTCAATGC 
AAGGAACGGA ATTTACAAGA TCCTGTGCCA AGGGAATCGC CAAAGTCTTG GTTTTTTGAC 
AGCCGATCAA AGTCTTTACC ATCACCTCAT CCAGTTCGCT ACTTTGATAA CTCGGGTTTA 
GGCCTTTGCC CAGAAGAACA AATTGCTGGA GCAGAGAATG TGGATCCTCA GCCTGGTCTA 
TGGCATGCCT CCTCAGGAAA AGATGTGCCC GAGTGCAAGA CCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPE ( XM_014110057.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965532.1 Canis lupus familiaris centromere protein E, 312kDa (CENPE), transcript variant X1, mRNA.

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CloneID	OCe44622
Clone ID Related Accession (Same CDS sequence)	XM_014110057.1 , XM_014110057.2
Accession Version	XM_014110057.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	centromere-associated protein E isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGATTT GGAAACACAA 
CAAGAACTAA AAATTGCTCT TTTGCATTTG AAAGAACACC AAGAAACTAT TGATAAATTC 
AGAGAGAGAG TTTCAGAAAA GACAACTCAG ATTTCAAATA TCCAAAAGGA TTTAAATAAA 
TCTAAAGATG AGTTACAGAA AAAGATAGAA GAACTTCAGA AAAAAGAGCT TCAACTTCTC 
AAAATGAAAG AAGATGTCAA TAAAACTCAT AAAAATATAA ACAAAATGGA ACAATTGAAG 
CAACAGTTTG AGGCCCAGAA CTTATCTATG CAAAGTGTGG AAATGGATAA CTTAGACTTG 
ACAAAGAAAC TTCATAAATG CCTTGAAGAA AAAAGAATTA TAGCTAAGGA AAGAGATGAG 
CTAAGGAGGA TAAAAGAGTC TCTCAAAATG GAAAGAGACC AATTCAGAGA AACCTTAACA 
GAGATGATAG CTAGCGACCA ACAGAACCAA AAAGAGATAG TAAAATATGA AAAAAGGTTA 
CAATGTAATG AAAAACACCA CCTCACAGAA AGCCTAAGGG AGAAGTGCTC TAGGATAAAA 
GAGCTTCTGA AGAGATACTC AGAGATGTAT AATCATTATG AGTGCTTAAC TAGACTATCC 
CTTGACTTGG AGAAAGAAAT TGAAACCCAA AAAGAGCTTT CAGTTACAAT AAAAGCAAAC 
CTCTCACTTC CACATTCACA AATCAAACAA ATTGAAAAAC TTCTCGCTGT AAACCAAAGA 
TGTTCAATGG AATTCCACAG AATCATCAAG AGATTTCAGT ACGTGTTAAG CCAGATTACA 
AAGATAAAGG AAGAACAACA TGAATCCATA AATAAATTAG AAGTGGTTTT TATTGATGAA 
GTGGAAAAGC ACAATGAACT GGTAATTAAA ATACAGCACC TTCAACAAGA TTTTAATGTA 
CCATCTGGAG AATTAACGGA CCTCAAATTG AGCCAGAAGA TGGATCTACG TATTGAGGAA 
ATTCTCAAAG ATCTTTCAGA AAATGATTTC CACTTACTAA AGACTGAATT TCAACAAGTG 
CTAAGTAATA GGAGAGAAAT AACCCAGTTT TTGGAAGAAT GGTTGAATAC TCATTTTGAT 
ATAGAAAAGC TTAAAAATGG CATCCAGAAA GAAAATGATA ATATTTGTCA AGTGAATAAC 
TTCTATAATA ACAAAATAAT TGCTATAATG AATGAATCTA CAGAGTTTGA GGAAAGATAT 
GCCATCATAG CCAAAGAATG GGAACGTGAC CTGAATTCAG TGAAAGAGAA AAATGAACAA 
CTATTTAAAA ACTATCAAAC TTTGAAGCTC ACCCGGACGT CTGGTATCCC GGTAAATTCT 
GCCACAGAAG ACAATAAGAA TCTTCATGTT ACATCAGGAG CCACACAACT AACCACAGAG 
AAAATTCAGG AGCTGGAAGC TTCACTGCAT GAAGCTAAAG AAAGTGCTTT GCACAAGGAA 
AACAAGATTG TAAAGATGCA GAAAGAACTT GAGATGACTA ATGACATCAT AGCAAAACTT 
CAGTCGCAAG TTAATGAATC AAATAAATGC CTTGAAAAAA CAAAAGAAAG GATCCAATTA 
CTTCAGGATA AAGTTGCTTT AGGAGCTAAG CCCTATAAAG AAGAAATTGA AGATCTCAAG 
ATGAAACTGG TGAAAATGGA TCTAGAAAAG AAGAAGAATG CCAAAGAGTT TGAAAAAGAA 
ATTACTTCTA CAAAGGCCAC TGTAGAACAT CAAAAAGAAG TTATAAGGGT ACTGAGAGAA 
AATCTCAGAA GAAATCAACA AGCCCAGGAT ACCTCAATTG TATCAGAATA TACGGACTCT 
CAGCCTCCAA ATAAACCCTT AACTTGTGGA GGTGGCAGTG GCATCGTACA AAGCACAAAA 
GCTCTTATTT TAAAAAGTGG ATATATACGG CTAGAAAAGG AAATCTCTAA GTTAAAACAG 
CAAAATGAAC AGCTAATAAA GCAAAAGAAT GAACTATTAA GCAATAACCA TCATCTTTCC 
AACGAAGTCA AAACATGGAA GGAGAGAATC CTTAAAAGAG AGGCTCACAG AGAAGTTACT 
TGTGAGAATT CTCCACAGTC TCCTAAAGTG ACTATAACAG CTCCCAAAAA GAGGCCGCAT 
ACGCCTTCTC AATGCAAGGA ACGGAATTTA CAAGATCCTG TGCCAAGGGA ATCGCCAAAG 
TCTTGGTTTT TTGACAGCCG ATCAAAGTCT TTACCATCAC CTCATCCAGT TCGCTACTTT 
GATAACTCGG GTTTAGGCCT TTGCCCAGAA GAACAAATTG CTGGAGCAGA GAATGTGGAT 
CCTCAGCCTG GTCTATGGCA TGCCTCCTCA GGAAAAGATG TGCCCGAGTG CAAGACCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965532.1
CDS	72..8354
Translation	MAEEGAVAVCVRVRPLNDREEALGGDTQIYWKTDNNAIYQVDGSKSFNFDRVFHSNETTKHVYEEIAVPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSQDYLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCDTQKMKPLIIREDFNRNVYVADLTEEVVYTSDMALKWITKGEKNRHYGITKMNQRSSRSHTIFRMILESREKGEPSNCDGSVKVSHLNLVDLAGSERAAQTGAEGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTVTPVSFDETLTTLQFASTAKYMKNTPYVNEVSSDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQTEKIQNLTRMLVTSSSLTSHQELKAKRKRRVTWCLGKINKMKDSNYINEFNMSTNITTRKAALTVVGEIDESLCSESDVFSNTFDTLNETEWNPATKLLSQENLESELNSLRANYDNLVLDYEQLRQEHEEMELKLKEKNDLDEFEALERKAEKDQEMQLIHEISNLKNLVKHAEVYNQDLENELSSKVELLREKEDQIKKLQKYIDSQKSEDIKTDLSYSSESTEDLKQMKQTLLDAETVVLDAKRESAFLRSENLELKEKMKELRSRYKQMENDIQLYQRQLEAKKKMQVDLEKELQSSFNEITKLTSLIDGKVPKGLLCNLELERKITDFQRELNEKVEENEALHKEVKLLSALKSLPSEIEMLRKEIHDKSEELYIITSEREKLNSEVVQKESRIQDLLEEIGKTKNDLATIRLNYESTDQEFQDFKNHHIELEQKYKMVLEENARMNQELGNLSEQAQKLGLSLDALSNTELSPKSEELLQKTTESQKRLNEVEELKEQLESTGSRLQTIEKEKTLIAEQLQQTLVEVRTLTQEKDDLKQLQESLQIERDQLKSDIQDTVNMNIDTQEQLRNALEALKQHQEVINTLRLKIAEKTSRHLETEENLGEAKDEFQEEMVDTDKKQNLKAKNTQALVVNVEDNELTEQQRNIFSLIQEKNELQRMLESVTAEKEQLKTDLRENIEMTIENQEELRILGDELKKQQEILVQEKNHTIKKEEELSRTYKKLAEVEEKLKEKNQQLQEKQLQLLSVQEEMSEMQKKMNEMENVNNEFRNRELTLERIEMERLEVAQKLHENDEEMKSLTKERNDLKELQESFELEREQLEGFIREIEVTIPVLHEEQELLPNVKEVSATQGTMNEGEILKKQSKTKDSMTPARIEIERLGLTAKLPESHEEIKSLTEERDNLKTIKEAVQVGQEQLKEDSREMLAEDSLNISEPQNKQEQSFDMKEREDEAEEIVSELEQLQEQLKAKEAPQPRVGMERLEMSEKLQEVLQSETNQLKENMREIIAKHLETEEELKVARCHLKEQEETIDKLRVNLSERETELSSLQKELEITNNELQKKIQELHEKQQFMSTKEITETQEKMSELEQLKEQLRVKDSSLQNIESEKLKLTEKLQESQDKIKIVIKERDELKRVQELLQMERDQLKANIKEIVAEEYQQTISTRNISKKTDQEANIERVIESSDAESQEKIQELQEKEHQLLKMKTVSETQEKICEMEHLKNQFEAQKSTLENIEMENARLTQRLHENLEEMRSVTKERDDLRSIEETLKVERDQLKENLRETVIRDLEKQEELRIARMHVKEHQETIDKLRGLVSEKTDELSNMQIDLENTNDALKTQIQELQEKEHQLLKVKNDLRENMYQTEQLKEQLQAQHSTLESVETERLRLSQKLHENLEEIKCVTEERDTLKRAERTLRMEQCQLRESLREKEAQDLEKQEELRIVCMHVKEHQETIDKLRGLVSEKTDEIANMQMDLENSNAKLQEKIQQLKANEHQLFKLKEDVSETQKKTSEIERLKKQLKGQSLTLDKIEMENLNLAQKLHENLEEMKSVMQERDNLKLERDQLKTNLQETIARDLETQQELKIALLHLKEHQETIDKFRERVSEKTTQISNIQKDLNKSKDELQKKIEELQKKELQLLKMKEDVNKTHKNINKMEQLKQQFEAQNLSMQSVEMDNLDLTKKLHKCLEEKRIIAKERDELRRIKESLKMERDQFRETLTEMIASDQQNQKEIVKYEKRLQCNEKHHLTESLREKCSRIKELLKRYSEMYNHYECLTRLSLDLEKEIETQKELSVTIKANLSLPHSQIKQIEKLLAVNQRCSMEFHRIIKRFQYVLSQITKIKEEQHESINKLEVVFIDEVEKHNELVIKIQHLQQDFNVPSGELTDLKLSQKMDLRIEEILKDLSENDFHLLKTEFQQVLSNRREITQFLEEWLNTHFDIEKLKNGIQKENDNICQVNNFYNNKIIAIMNESTEFEERYAIIAKEWERDLNSVKEKNEQLFKNYQTLKLTRTSGIPVNSATEDNKNLHVTSGATQLTTEKIQELEASLHEAKESALHKENKIVKMQKELEMTNDIIAKLQSQVNESNKCLEKTKERIQLLQDKVALGAKPYKEEIEDLKMKLVKMDLEKKKNAKEFEKEITSTKATVEHQKEVIRVLRENLRRNQQAQDTSIVSEYTDSQPPNKPLTCGGGSGIVQSTKALILKSGYIRLEKEISKLKQQNEQLIKQKNELLSNNHHLSNEVKTWKERILKREAHREVTCENSPQSPKVTITAPKKRPHTPSQCKERNLQDPVPRESPKSWFFDSRSKSLPSPHPVRYFDNSGLGLCPEEQIAGAENVDPQPGLWHASSGKDVPECKTQ

Target ORF information:

RefSeq Version	XM_014110057.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris centromere protein E, 312kDa (CENPE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110057.1

ORF Insert Sequence:

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGATTT GGAAACACAA 
CAAGAACTAA AAATTGCTCT TTTGCATTTG AAAGAACACC AAGAAACTAT TGATAAATTC 
AGAGAGAGAG TTTCAGAAAA GACAACTCAG ATTTCAAATA TCCAAAAGGA TTTAAATAAA 
TCTAAAGATG AGTTACAGAA AAAGATAGAA GAACTTCAGA AAAAAGAGCT TCAACTTCTC 
AAAATGAAAG AAGATGTCAA TAAAACTCAT AAAAATATAA ACAAAATGGA ACAATTGAAG 
CAACAGTTTG AGGCCCAGAA CTTATCTATG CAAAGTGTGG AAATGGATAA CTTAGACTTG 
ACAAAGAAAC TTCATAAATG CCTTGAAGAA AAAAGAATTA TAGCTAAGGA AAGAGATGAG 
CTAAGGAGGA TAAAAGAGTC TCTCAAAATG GAAAGAGACC AATTCAGAGA AACCTTAACA 
GAGATGATAG CTAGCGACCA ACAGAACCAA AAAGAGATAG TAAAATATGA AAAAAGGTTA 
CAATGTAATG AAAAACACCA CCTCACAGAA AGCCTAAGGG AGAAGTGCTC TAGGATAAAA 
GAGCTTCTGA AGAGATACTC AGAGATGTAT AATCATTATG AGTGCTTAAC TAGACTATCC 
CTTGACTTGG AGAAAGAAAT TGAAACCCAA AAAGAGCTTT CAGTTACAAT AAAAGCAAAC 
CTCTCACTTC CACATTCACA AATCAAACAA ATTGAAAAAC TTCTCGCTGT AAACCAAAGA 
TGTTCAATGG AATTCCACAG AATCATCAAG AGATTTCAGT ACGTGTTAAG CCAGATTACA 
AAGATAAAGG AAGAACAACA TGAATCCATA AATAAATTAG AAGTGGTTTT TATTGATGAA 
GTGGAAAAGC ACAATGAACT GGTAATTAAA ATACAGCACC TTCAACAAGA TTTTAATGTA 
CCATCTGGAG AATTAACGGA CCTCAAATTG AGCCAGAAGA TGGATCTACG TATTGAGGAA 
ATTCTCAAAG ATCTTTCAGA AAATGATTTC CACTTACTAA AGACTGAATT TCAACAAGTG 
CTAAGTAATA GGAGAGAAAT AACCCAGTTT TTGGAAGAAT GGTTGAATAC TCATTTTGAT 
ATAGAAAAGC TTAAAAATGG CATCCAGAAA GAAAATGATA ATATTTGTCA AGTGAATAAC 
TTCTATAATA ACAAAATAAT TGCTATAATG AATGAATCTA CAGAGTTTGA GGAAAGATAT 
GCCATCATAG CCAAAGAATG GGAACGTGAC CTGAATTCAG TGAAAGAGAA AAATGAACAA 
CTATTTAAAA ACTATCAAAC TTTGAAGCTC ACCCGGACGT CTGGTATCCC GGTAAATTCT 
GCCACAGAAG ACAATAAGAA TCTTCATGTT ACATCAGGAG CCACACAACT AACCACAGAG 
AAAATTCAGG AGCTGGAAGC TTCACTGCAT GAAGCTAAAG AAAGTGCTTT GCACAAGGAA 
AACAAGATTG TAAAGATGCA GAAAGAACTT GAGATGACTA ATGACATCAT AGCAAAACTT 
CAGTCGCAAG TTAATGAATC AAATAAATGC CTTGAAAAAA CAAAAGAAAG GATCCAATTA 
CTTCAGGATA AAGTTGCTTT AGGAGCTAAG CCCTATAAAG AAGAAATTGA AGATCTCAAG 
ATGAAACTGG TGAAAATGGA TCTAGAAAAG AAGAAGAATG CCAAAGAGTT TGAAAAAGAA 
ATTACTTCTA CAAAGGCCAC TGTAGAACAT CAAAAAGAAG TTATAAGGGT ACTGAGAGAA 
AATCTCAGAA GAAATCAACA AGCCCAGGAT ACCTCAATTG TATCAGAATA TACGGACTCT 
CAGCCTCCAA ATAAACCCTT AACTTGTGGA GGTGGCAGTG GCATCGTACA AAGCACAAAA 
GCTCTTATTT TAAAAAGTGG ATATATACGG CTAGAAAAGG AAATCTCTAA GTTAAAACAG 
CAAAATGAAC AGCTAATAAA GCAAAAGAAT GAACTATTAA GCAATAACCA TCATCTTTCC 
AACGAAGTCA AAACATGGAA GGAGAGAATC CTTAAAAGAG AGGCTCACAG AGAAGTTACT 
TGTGAGAATT CTCCACAGTC TCCTAAAGTG ACTATAACAG CTCCCAAAAA GAGGCCGCAT 
ACGCCTTCTC AATGCAAGGA ACGGAATTTA CAAGATCCTG TGCCAAGGGA ATCGCCAAAG 
TCTTGGTTTT TTGACAGCCG ATCAAAGTCT TTACCATCAC CTCATCCAGT TCGCTACTTT 
GATAACTCGG GTTTAGGCCT TTGCCCAGAA GAACAAATTG CTGGAGCAGA GAATGTGGAT 
CCTCAGCCTG GTCTATGGCA TGCCTCCTCA GGAAAAGATG TGCCCGAGTG CAAGACCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPE ( XM_014110057.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965532.1 Canis lupus familiaris centromere protein E (CENPE), transcript variant X1, mRNA.

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CloneID	OCe44622
Clone ID Related Accession (Same CDS sequence)	XM_014110057.1 , XM_014110057.2
Accession Version	XM_014110057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	centromere-associated protein E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGATTT GGAAACACAA 
CAAGAACTAA AAATTGCTCT TTTGCATTTG AAAGAACACC AAGAAACTAT TGATAAATTC 
AGAGAGAGAG TTTCAGAAAA GACAACTCAG ATTTCAAATA TCCAAAAGGA TTTAAATAAA 
TCTAAAGATG AGTTACAGAA AAAGATAGAA GAACTTCAGA AAAAAGAGCT TCAACTTCTC 
AAAATGAAAG AAGATGTCAA TAAAACTCAT AAAAATATAA ACAAAATGGA ACAATTGAAG 
CAACAGTTTG AGGCCCAGAA CTTATCTATG CAAAGTGTGG AAATGGATAA CTTAGACTTG 
ACAAAGAAAC TTCATAAATG CCTTGAAGAA AAAAGAATTA TAGCTAAGGA AAGAGATGAG 
CTAAGGAGGA TAAAAGAGTC TCTCAAAATG GAAAGAGACC AATTCAGAGA AACCTTAACA 
GAGATGATAG CTAGCGACCA ACAGAACCAA AAAGAGATAG TAAAATATGA AAAAAGGTTA 
CAATGTAATG AAAAACACCA CCTCACAGAA AGCCTAAGGG AGAAGTGCTC TAGGATAAAA 
GAGCTTCTGA AGAGATACTC AGAGATGTAT AATCATTATG AGTGCTTAAC TAGACTATCC 
CTTGACTTGG AGAAAGAAAT TGAAACCCAA AAAGAGCTTT CAGTTACAAT AAAAGCAAAC 
CTCTCACTTC CACATTCACA AATCAAACAA ATTGAAAAAC TTCTCGCTGT AAACCAAAGA 
TGTTCAATGG AATTCCACAG AATCATCAAG AGATTTCAGT ACGTGTTAAG CCAGATTACA 
AAGATAAAGG AAGAACAACA TGAATCCATA AATAAATTAG AAGTGGTTTT TATTGATGAA 
GTGGAAAAGC ACAATGAACT GGTAATTAAA ATACAGCACC TTCAACAAGA TTTTAATGTA 
CCATCTGGAG AATTAACGGA CCTCAAATTG AGCCAGAAGA TGGATCTACG TATTGAGGAA 
ATTCTCAAAG ATCTTTCAGA AAATGATTTC CACTTACTAA AGACTGAATT TCAACAAGTG 
CTAAGTAATA GGAGAGAAAT AACCCAGTTT TTGGAAGAAT GGTTGAATAC TCATTTTGAT 
ATAGAAAAGC TTAAAAATGG CATCCAGAAA GAAAATGATA ATATTTGTCA AGTGAATAAC 
TTCTATAATA ACAAAATAAT TGCTATAATG AATGAATCTA CAGAGTTTGA GGAAAGATAT 
GCCATCATAG CCAAAGAATG GGAACGTGAC CTGAATTCAG TGAAAGAGAA AAATGAACAA 
CTATTTAAAA ACTATCAAAC TTTGAAGCTC ACCCGGACGT CTGGTATCCC GGTAAATTCT 
GCCACAGAAG ACAATAAGAA TCTTCATGTT ACATCAGGAG CCACACAACT AACCACAGAG 
AAAATTCAGG AGCTGGAAGC TTCACTGCAT GAAGCTAAAG AAAGTGCTTT GCACAAGGAA 
AACAAGATTG TAAAGATGCA GAAAGAACTT GAGATGACTA ATGACATCAT AGCAAAACTT 
CAGTCGCAAG TTAATGAATC AAATAAATGC CTTGAAAAAA CAAAAGAAAG GATCCAATTA 
CTTCAGGATA AAGTTGCTTT AGGAGCTAAG CCCTATAAAG AAGAAATTGA AGATCTCAAG 
ATGAAACTGG TGAAAATGGA TCTAGAAAAG AAGAAGAATG CCAAAGAGTT TGAAAAAGAA 
ATTACTTCTA CAAAGGCCAC TGTAGAACAT CAAAAAGAAG TTATAAGGGT ACTGAGAGAA 
AATCTCAGAA GAAATCAACA AGCCCAGGAT ACCTCAATTG TATCAGAATA TACGGACTCT 
CAGCCTCCAA ATAAACCCTT AACTTGTGGA GGTGGCAGTG GCATCGTACA AAGCACAAAA 
GCTCTTATTT TAAAAAGTGG ATATATACGG CTAGAAAAGG AAATCTCTAA GTTAAAACAG 
CAAAATGAAC AGCTAATAAA GCAAAAGAAT GAACTATTAA GCAATAACCA TCATCTTTCC 
AACGAAGTCA AAACATGGAA GGAGAGAATC CTTAAAAGAG AGGCTCACAG AGAAGTTACT 
TGTGAGAATT CTCCACAGTC TCCTAAAGTG ACTATAACAG CTCCCAAAAA GAGGCCGCAT 
ACGCCTTCTC AATGCAAGGA ACGGAATTTA CAAGATCCTG TGCCAAGGGA ATCGCCAAAG 
TCTTGGTTTT TTGACAGCCG ATCAAAGTCT TTACCATCAC CTCATCCAGT TCGCTACTTT 
GATAACTCGG GTTTAGGCCT TTGCCCAGAA GAACAAATTG CTGGAGCAGA GAATGTGGAT 
CCTCAGCCTG GTCTATGGCA TGCCTCCTCA GGAAAAGATG TGCCCGAGTG CAAGACCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965532.1
CDS	99..8381
Translation	MAEEGAVAVCVRVRPLNDREEALGGDTQIYWKTDNNAIYQVDGSKSFNFDRVFHSNETTKHVYEEIAVPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSQDYLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCDTQKMKPLIIREDFNRNVYVADLTEEVVYTSDMALKWITKGEKNRHYGITKMNQRSSRSHTIFRMILESREKGEPSNCDGSVKVSHLNLVDLAGSERAAQTGAEGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTVTPVSFDETLTTLQFASTAKYMKNTPYVNEVSSDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQTEKIQNLTRMLVTSSSLTSHQELKAKRKRRVTWCLGKINKMKDSNYINEFNMSTNITTRKAALTVVGEIDESLCSESDVFSNTFDTLNETEWNPATKLLSQENLESELNSLRANYDNLVLDYEQLRQEHEEMELKLKEKNDLDEFEALERKAEKDQEMQLIHEISNLKNLVKHAEVYNQDLENELSSKVELLREKEDQIKKLQKYIDSQKSEDIKTDLSYSSESTEDLKQMKQTLLDAETVVLDAKRESAFLRSENLELKEKMKELRSRYKQMENDIQLYQRQLEAKKKMQVDLEKELQSSFNEITKLTSLIDGKVPKGLLCNLELERKITDFQRELNEKVEENEALHKEVKLLSALKSLPSEIEMLRKEIHDKSEELYIITSEREKLNSEVVQKESRIQDLLEEIGKTKNDLATIRLNYESTDQEFQDFKNHHIELEQKYKMVLEENARMNQELGNLSEQAQKLGLSLDALSNTELSPKSEELLQKTTESQKRLNEVEELKEQLESTGSRLQTIEKEKTLIAEQLQQTLVEVRTLTQEKDDLKQLQESLQIERDQLKSDIQDTVNMNIDTQEQLRNALEALKQHQEVINTLRLKIAEKTSRHLETEENLGEAKDEFQEEMVDTDKKQNLKAKNTQALVVNVEDNELTEQQRNIFSLIQEKNELQRMLESVTAEKEQLKTDLRENIEMTIENQEELRILGDELKKQQEILVQEKNHTIKKEEELSRTYKKLAEVEEKLKEKNQQLQEKQLQLLSVQEEMSEMQKKMNEMENVNNEFRNRELTLERIEMERLEVAQKLHENDEEMKSLTKERNDLKELQESFELEREQLEGFIREIEVTIPVLHEEQELLPNVKEVSATQGTMNEGEILKKQSKTKDSMTPARIEIERLGLTAKLPESHEEIKSLTEERDNLKTIKEAVQVGQEQLKEDSREMLAEDSLNISEPQNKQEQSFDMKEREDEAEEIVSELEQLQEQLKAKEAPQPRVGMERLEMSEKLQEVLQSETNQLKENMREIIAKHLETEEELKVARCHLKEQEETIDKLRVNLSERETELSSLQKELEITNNELQKKIQELHEKQQFMSTKEITETQEKMSELEQLKEQLRVKDSSLQNIESEKLKLTEKLQESQDKIKIVIKERDELKRVQELLQMERDQLKANIKEIVAEEYQQTISTRNISKKTDQEANIERVIESSDAESQEKIQELQEKEHQLLKMKTVSETQEKICEMEHLKNQFEAQKSTLENIEMENARLTQRLHENLEEMRSVTKERDDLRSIEETLKVERDQLKENLRETVIRDLEKQEELRIARMHVKEHQETIDKLRGLVSEKTDELSNMQIDLENTNDALKTQIQELQEKEHQLLKVKNDLRENMYQTEQLKEQLQAQHSTLESVETERLRLSQKLHENLEEIKCVTEERDTLKRAERTLRMEQCQLRESLREKEAQDLEKQEELRIVCMHVKEHQETIDKLRGLVSEKTDEIANMQMDLENSNAKLQEKIQQLKANEHQLFKLKEDVSETQKKTSEIERLKKQLKGQSLTLDKIEMENLNLAQKLHENLEEMKSVMQERDNLKLERDQLKTNLQETIARDLETQQELKIALLHLKEHQETIDKFRERVSEKTTQISNIQKDLNKSKDELQKKIEELQKKELQLLKMKEDVNKTHKNINKMEQLKQQFEAQNLSMQSVEMDNLDLTKKLHKCLEEKRIIAKERDELRRIKESLKMERDQFRETLTEMIASDQQNQKEIVKYEKRLQCNEKHHLTESLREKCSRIKELLKRYSEMYNHYECLTRLSLDLEKEIETQKELSVTIKANLSLPHSQIKQIEKLLAVNQRCSMEFHRIIKRFQYVLSQITKIKEEQHESINKLEVVFIDEVEKHNELVIKIQHLQQDFNVPSGELTDLKLSQKMDLRIEEILKDLSENDFHLLKTEFQQVLSNRREITQFLEEWLNTHFDIEKLKNGIQKENDNICQVNNFYNNKIIAIMNESTEFEERYAIIAKEWERDLNSVKEKNEQLFKNYQTLKLTRTSGIPVNSATEDNKNLHVTSGATQLTTEKIQELEASLHEAKESALHKENKIVKMQKELEMTNDIIAKLQSQVNESNKCLEKTKERIQLLQDKVALGAKPYKEEIEDLKMKLVKMDLEKKKNAKEFEKEITSTKATVEHQKEVIRVLRENLRRNQQAQDTSIVSEYTDSQPPNKPLTCGGGSGIVQSTKALILKSGYIRLEKEISKLKQQNEQLIKQKNELLSNNHHLSNEVKTWKERILKREAHREVTCENSPQSPKVTITAPKKRPHTPSQCKERNLQDPVPRESPKSWFFDSRSKSLPSPHPVRYFDNSGLGLCPEEQIAGAENVDPQPGLWHASSGKDVPECKTQ

Target ORF information:

RefSeq Version	XM_014110057.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centromere protein E (CENPE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110057.2

ORF Insert Sequence:

ATGGCGGAGG AAGGGGCTGT CGCGGTGTGC GTGCGGGTCC GTCCGCTCAA CGACAGAGAA 
GAAGCTCTTG GAGGAGATAC CCAAATTTAC TGGAAAACTG ACAATAATGC CATTTATCAA 
GTTGATGGCA GTAAATCCTT CAATTTTGAT CGTGTTTTTC ACAGTAATGA AACTACTAAA 
CATGTGTATG AAGAAATAGC AGTCCCAATC ATAGATTCTG CCATACAAGG CTACAATGGT 
ACCATATTTG CCTATGGGCA GACTGCTTCA GGAAAAACAT ACACGATGAT GGGTTCACAG 
GATTATTTGG GAGTAATACC CAGGGCAATT CATGACATTT TTCAAAAAAT TAAAAAGTTT 
CCTGATAGGG AATTTCTCTT ACGTGTGTCT TACATGGAGA TATACAATGA AACCATTACA 
GACTTACTCT GTGACACTCA AAAAATGAAG CCTTTAATAA TTCGGGAAGA CTTCAATAGG 
AATGTGTACG TGGCTGATCT CACAGAAGAA GTTGTTTATA CATCAGATAT GGCTTTGAAG 
TGGATCACAA AGGGAGAAAA GAACAGACAT TATGGAATTA CAAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACCATATT TAGGATGATT TTGGAAAGTA GAGAGAAAGG GGAACCTTCC 
AATTGTGACG GATCTGTCAA AGTGTCCCAT TTGAATTTGG TTGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGCGC TGAAGGTGTG CGACTCAAAG AAGGCTGTAA TATAAATCGA 
AGTTTATTTA TTTTGGGACA AGTAATCAAG AAACTTAGTG ATGGACAAGT TGGTGGTTTC 
ATAAATTATC GAGATAGTAA ATTAACACGA ATTCTCCAGA ATTCCTTGGG TGGAAATGCA 
AAAACACGTA TCATCTGCAC GGTTACTCCA GTGTCTTTTG ATGAAACCCT TACAACTCTA 
CAGTTTGCAA GCACTGCCAA GTATATGAAG AATACTCCTT ATGTTAATGA GGTATCAAGT 
GATGAAGCTC TCTTGAAAAG ATACAGAAAA GAAATAATGG ATCTTAAGAA ACAGTTGGAG 
GAGGTTTCCT TAGAGACTCG GGCCCAGGCA ATGGAAAAAG ACCAGTTGGC CCAACTTTTA 
GAAGAAAAAG ATTTACTTCA AAAAGTACAG ACTGAGAAAA TTCAGAACTT AACACGGATG 
CTGGTGACCT CTTCTTCCCT CACATCACAT CAGGAATTAA AGGCTAAAAG AAAACGAAGA 
GTCACTTGGT GTCTTGGCAA AATTAACAAA ATGAAGGACT CAAATTATAT AAATGAATTT 
AATATGTCAA CAAATATAAC AACACGTAAG GCAGCTCTAA CAGTAGTGGG CGAAATTGAT 
GAATCTCTCT GCTCAGAGTC TGATGTTTTC AGTAATACTT TTGATACATT AAATGAAACA 
GAGTGGAATC CAGCAACAAA GCTACTAAGT CAGGAGAACT TAGAAAGTGA GCTGAATTCA 
CTTCGTGCCA ACTATGATAA TCTGGTATTG GACTATGAAC AACTAAGACA AGAACATGAA 
GAAATGGAAT TGAAATTAAA AGAAAAGAAT GATTTGGATG AATTTGAGGC TCTAGAAAGA 
AAAGCAGAAA AAGATCAGGA GATGCAACTA ATTCATGAAA TTTCCAACTT AAAGAATTTA 
GTTAAGCATG CAGAGGTGTA TAATCAAGAC CTTGAGAATG AACTAAGTTC AAAAGTAGAG 
CTGCTTAGAG AAAAGGAAGA CCAGATTAAG AAGCTACAGA AATACATAGA CTCTCAGAAG 
TCAGAAGATA TTAAAACGGA CTTGTCCTAC TCATCGGAAA GCACTGAAGA CCTAAAACAA 
ATGAAACAGA CTTTACTTGA TGCTGAAACT GTAGTCCTTG ATGCTAAGAG AGAATCAGCC 
TTTCTTAGAA GTGAAAATCT GGAGCTGAAA GAGAAAATGA AAGAACTTAG AAGTAGATAC 
AAGCAAATGG AGAATGATAT TCAGTTATAT CAAAGACAGT TGGAGGCAAA AAAGAAAATG 
CAAGTTGATC TGGAAAAAGA ATTACAATCT TCATTTAATG AGATAACGAA ACTCACCTCC 
CTTATTGATG GCAAAGTTCC AAAAGGTTTG CTCTGTAATT TGGAACTGGA AAGAAAGATT 
ACTGATTTCC AGAGAGAACT AAATGAAAAA GTTGAAGAAA ATGAAGCTTT GCATAAAGAA 
GTTAAGTTGC TCTCAGCGTT GAAATCTTTA CCCTCAGAAA TAGAAATGCT GAGGAAAGAG 
ATACATGATA AATCTGAAGA GCTCTATATA ATAACATCGG AAAGAGAGAA ATTGAATTCT 
GAAGTAGTTC AAAAGGAAAG TAGAATTCAA GATTTACTTG AAGAAATTGG GAAAACTAAA 
AATGATCTAG CAACTATCCG GTTGAATTAT GAAAGCACTG ATCAAGAATT CCAAGATTTT 
AAAAATCATC ATATTGAATT AGAGCAAAAG TATAAGATGG TCCTTGAAGA GAATGCAAGA 
ATGAATCAGG AATTAGGAAA TCTCTCTGAA CAAGCTCAAA AACTTGGTTT AAGTTTGGAT 
GCTTTGAGCA ACACAGAGCT TTCTCCCAAA TCTGAAGAAC TTCTACAAAA AACAACTGAG 
AGTCAAAAAA GATTAAATGA AGTGGAAGAG CTGAAGGAAC AATTAGAAAG TACAGGTTCA 
AGGCTGCAAA CTATAGAAAA GGAAAAAACA CTGATTGCTG AGCAACTTCA GCAAACCTTG 
GTAGAAGTAA GAACCTTAAC CCAAGAAAAA GATGACCTAA AACAACTCCA GGAGAGCCTG 
CAGATTGAGA GGGACCAACT CAAAAGTGAC ATTCAGGATA CTGTAAACAT GAATATAGAC 
ACCCAAGAAC AATTACGAAA TGCTCTTGAA GCTTTGAAAC AACACCAAGA AGTAATTAAC 
ACACTAAGAC TGAAAATTGC TGAGAAAACT TCCAGGCATT TGGAAACAGA GGAAAACCTA 
GGAGAAGCTA AGGATGAATT CCAGGAAGAG ATGGTTGACA CAGATAAAAA ACAGAATTTG 
AAAGCCAAAA ATACTCAAGC ACTGGTTGTG AATGTTGAAG ATAATGAGCT AACTGAGCAA 
CAGAGAAATA TATTTTCTTT AATACAGGAG AAGAATGAAC TGCAACGAAT GTTAGAGAGT 
GTTACAGCAG AAAAGGAACA GTTAAAGACT GACTTAAGGG AAAATATTGA AATGACTATT 
GAAAACCAAG AAGAATTGAG AATTCTTGGA GATGAACTTA AAAAGCAACA AGAGATACTT 
GTACAAGAAA AGAACCACAC TATAAAGAAA GAAGAAGAAC TCTCTAGGAC CTATAAGAAA 
TTAGCAGAAG TTGAAGAAAA GTTAAAGGAA AAGAACCAAC AACTACAAGA AAAACAGCTA 
CAACTTCTTA GTGTACAAGA AGAAATGAGT GAGATGCAGA AAAAGATGAA TGAAATGGAG 
AATGTAAACA ATGAATTTAG GAACCGAGAA CTGACATTGG AACGTATAGA AATGGAGAGG 
CTAGAGGTGG CTCAGAAGCT CCATGAAAAT GATGAGGAAA TGAAATCATT AACCAAAGAA 
AGAAATGATC TAAAGGAATT ACAGGAATCA TTTGAATTAG AGAGAGAGCA ACTTGAAGGA 
TTCATAAGAG AAATCGAAGT TACAATCCCA GTGCTTCATG AGGAACAGGA GCTACTGCCT 
AATGTGAAAG AAGTCAGTGC TACTCAGGGA ACAATGAATG AAGGGGAGAT ATTGAAAAAG 
CAGTCCAAAA CCAAGGATTC AATGACACCA GCACGCATAG AAATAGAAAG GCTTGGGTTG 
ACTGCAAAAC TTCCAGAAAG TCATGAAGAA ATAAAATCTC TAACTGAGGA AAGAGACAAC 
CTTAAAACAA TAAAAGAAGC AGTTCAGGTT GGACAAGAGC AACTGAAAGA AGACAGTAGA 
GAAATGTTGG CTGAAGATTC TCTTAATATC TCAGAACCTC AAAATAAACA AGAACAGTCC 
TTTGACATGA AAGAAAGAGA AGATGAAGCT GAGGAAATCG TGAGTGAGTT GGAGCAACTG 
CAGGAGCAGC TTAAAGCCAA AGAGGCACCA CAGCCAAGAG TGGGCATGGA AAGGCTTGAG 
ATGTCTGAGA AGCTACAAGA AGTTCTTCAG TCTGAAACTA ACCAACTCAA AGAAAACATG 
AGAGAAATCA TAGCTAAACA CCTGGAAACT GAAGAGGAAC TGAAAGTTGC TCGTTGTCAC 
CTGAAAGAAC AAGAGGAAAC TATTGATAAG CTGAGAGTGA ATCTTTCAGA GAGGGAAACT 
GAACTATCCA GCCTTCAAAA GGAATTAGAA ATAACCAATA ATGAATTACA GAAAAAGATC 
CAAGAGCTTC ATGAGAAACA GCAATTTATG AGCACCAAAG AAATCACTGA GACTCAGGAA 
AAAATGAGTG AACTGGAGCA ATTAAAGGAG CAACTCAGAG TCAAAGACTC ATCATTACAA 
AATATAGAAA GTGAGAAGCT CAAGTTGACT GAGAAACTTC AGGAAAGTCA AGATAAAATA 
AAAATTGTAA TTAAGGAAAG AGATGAACTG AAAAGAGTAC AGGAACTTCT TCAAATGGAG 
AGAGACCAAC TCAAAGCAAA CATTAAAGAA ATTGTAGCTG AGGAGTACCA ACAAACCATT 
AGTACTAGGA ATATTTCAAA GAAGACAGAC CAAGAAGCAA ATATTGAGAG AGTTATAGAA 
AGTTCAGATG CTGAATCACA AGAAAAGATT CAAGAACTTC AGGAGAAAGA GCATCAGCTT 
CTTAAGATGA AAACTGTCAG TGAGACTCAG GAAAAAATAT GTGAGATGGA ACACTTGAAG 
AACCAGTTTG AGGCCCAAAA GTCAACTCTG GAAAATATAG AAATGGAGAA TGCAAGGTTA 
ACTCAGAGAC TTCATGAAAA CCTTGAAGAA ATGAGATCTG TTACAAAAGA AAGAGATGAT 
CTTAGGAGTA TAGAGGAGAC TCTCAAAGTA GAGAGAGACC AGCTCAAGGA AAACCTGAGA 
GAAACTGTAA TTAGAGACCT GGAAAAACAA GAGGAACTAA GAATTGCTCG CATGCATGTG 
AAAGAGCACC AGGAAACTAT TGATAAACTC AGGGGCCTTG TTTCTGAGAA GACAGATGAA 
CTATCAAATA TGCAGATAGA TTTAGAAAAC ACAAATGATG CCTTAAAAAC ACAGATCCAA 
GAACTTCAGG AGAAAGAACA TCAACTTCTT AAAGTAAAAA ATGATCTCAG AGAAAATATG 
TATCAAACAG AGCAATTGAA GGAGCAATTA CAGGCCCAAC ATTCAACCCT GGAAAGTGTA 
GAAACTGAGA GGCTGAGGTT AAGTCAGAAA CTTCATGAAA ACCTTGAAGA AATAAAATGT 
GTTACTGAGG AAAGAGACAC TCTAAAAAGA GCAGAGAGAA CCCTCAGGAT GGAGCAATGC 
CAGCTCAGAG AAAGCCTCAG AGAAAAGGAA GCTCAAGATC TGGAAAAACA AGAAGAACTA 
AGAATTGTTT GCATGCATGT GAAAGAGCAC CAGGAAACTA TTGATAAACT CAGAGGCCTT 
GTTTCTGAGA AGACAGATGA AATAGCAAAT ATGCAAATGG ATTTAGAAAA TTCAAATGCT 
AAATTACAAG AAAAGATTCA ACAACTTAAG GCAAATGAAC ATCAACTTTT TAAGCTAAAG 
GAGGATGTCA GTGAGACACA GAAAAAGACG TCTGAAATAG AGCGATTGAA GAAACAATTG 
AAGGGCCAAA GTTTAACTCT GGATAAAATA GAAATGGAGA ACTTAAATTT GGCTCAGAAA 
CTTCATGAAA ACCTTGAAGA AATGAAATCT GTAATGCAAG AAAGAGATAA TCTGAAATTG 
GAGAGAGACC AGCTCAAGAC AAACCTACAG GAAACCATAG CCAGAGATTT GGAAACACAA 
CAAGAACTAA AAATTGCTCT TTTGCATTTG AAAGAACACC AAGAAACTAT TGATAAATTC 
AGAGAGAGAG TTTCAGAAAA GACAACTCAG ATTTCAAATA TCCAAAAGGA TTTAAATAAA 
TCTAAAGATG AGTTACAGAA AAAGATAGAA GAACTTCAGA AAAAAGAGCT TCAACTTCTC 
AAAATGAAAG AAGATGTCAA TAAAACTCAT AAAAATATAA ACAAAATGGA ACAATTGAAG 
CAACAGTTTG AGGCCCAGAA CTTATCTATG CAAAGTGTGG AAATGGATAA CTTAGACTTG 
ACAAAGAAAC TTCATAAATG CCTTGAAGAA AAAAGAATTA TAGCTAAGGA AAGAGATGAG 
CTAAGGAGGA TAAAAGAGTC TCTCAAAATG GAAAGAGACC AATTCAGAGA AACCTTAACA 
GAGATGATAG CTAGCGACCA ACAGAACCAA AAAGAGATAG TAAAATATGA AAAAAGGTTA 
CAATGTAATG AAAAACACCA CCTCACAGAA AGCCTAAGGG AGAAGTGCTC TAGGATAAAA 
GAGCTTCTGA AGAGATACTC AGAGATGTAT AATCATTATG AGTGCTTAAC TAGACTATCC 
CTTGACTTGG AGAAAGAAAT TGAAACCCAA AAAGAGCTTT CAGTTACAAT AAAAGCAAAC 
CTCTCACTTC CACATTCACA AATCAAACAA ATTGAAAAAC TTCTCGCTGT AAACCAAAGA 
TGTTCAATGG AATTCCACAG AATCATCAAG AGATTTCAGT ACGTGTTAAG CCAGATTACA 
AAGATAAAGG AAGAACAACA TGAATCCATA AATAAATTAG AAGTGGTTTT TATTGATGAA 
GTGGAAAAGC ACAATGAACT GGTAATTAAA ATACAGCACC TTCAACAAGA TTTTAATGTA 
CCATCTGGAG AATTAACGGA CCTCAAATTG AGCCAGAAGA TGGATCTACG TATTGAGGAA 
ATTCTCAAAG ATCTTTCAGA AAATGATTTC CACTTACTAA AGACTGAATT TCAACAAGTG 
CTAAGTAATA GGAGAGAAAT AACCCAGTTT TTGGAAGAAT GGTTGAATAC TCATTTTGAT 
ATAGAAAAGC TTAAAAATGG CATCCAGAAA GAAAATGATA ATATTTGTCA AGTGAATAAC 
TTCTATAATA ACAAAATAAT TGCTATAATG AATGAATCTA CAGAGTTTGA GGAAAGATAT 
GCCATCATAG CCAAAGAATG GGAACGTGAC CTGAATTCAG TGAAAGAGAA AAATGAACAA 
CTATTTAAAA ACTATCAAAC TTTGAAGCTC ACCCGGACGT CTGGTATCCC GGTAAATTCT 
GCCACAGAAG ACAATAAGAA TCTTCATGTT ACATCAGGAG CCACACAACT AACCACAGAG 
AAAATTCAGG AGCTGGAAGC TTCACTGCAT GAAGCTAAAG AAAGTGCTTT GCACAAGGAA 
AACAAGATTG TAAAGATGCA GAAAGAACTT GAGATGACTA ATGACATCAT AGCAAAACTT 
CAGTCGCAAG TTAATGAATC AAATAAATGC CTTGAAAAAA CAAAAGAAAG GATCCAATTA 
CTTCAGGATA AAGTTGCTTT AGGAGCTAAG CCCTATAAAG AAGAAATTGA AGATCTCAAG 
ATGAAACTGG TGAAAATGGA TCTAGAAAAG AAGAAGAATG CCAAAGAGTT TGAAAAAGAA 
ATTACTTCTA CAAAGGCCAC TGTAGAACAT CAAAAAGAAG TTATAAGGGT ACTGAGAGAA 
AATCTCAGAA GAAATCAACA AGCCCAGGAT ACCTCAATTG TATCAGAATA TACGGACTCT 
CAGCCTCCAA ATAAACCCTT AACTTGTGGA GGTGGCAGTG GCATCGTACA AAGCACAAAA 
GCTCTTATTT TAAAAAGTGG ATATATACGG CTAGAAAAGG AAATCTCTAA GTTAAAACAG 
CAAAATGAAC AGCTAATAAA GCAAAAGAAT GAACTATTAA GCAATAACCA TCATCTTTCC 
AACGAAGTCA AAACATGGAA GGAGAGAATC CTTAAAAGAG AGGCTCACAG AGAAGTTACT 
TGTGAGAATT CTCCACAGTC TCCTAAAGTG ACTATAACAG CTCCCAAAAA GAGGCCGCAT 
ACGCCTTCTC AATGCAAGGA ACGGAATTTA CAAGATCCTG TGCCAAGGGA ATCGCCAAAG 
TCTTGGTTTT TTGACAGCCG ATCAAAGTCT TTACCATCAC CTCATCCAGT TCGCTACTTT 
GATAACTCGG GTTTAGGCCT TTGCCCAGAA GAACAAATTG CTGGAGCAGA GAATGTGGAT 
CCTCAGCCTG GTCTATGGCA TGCCTCCTCA GGAAAAGATG TGCCCGAGTG CAAGACCCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CENPE cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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