SPB4 cDNA ORF clone, Candida albicans SC5314

Gene
Clones
gRNAs

Summary

Gene Symbol	SPB4
Entrez Gene ID	3647252
Full Name	putative ATP-dependent RNA helicase
Synonyms	CaO19.6298
Gene Type	protein-coding
Organism	Candida albicans SC5314

mRNA and Protein(s)

mRNA	Protein	Name
XM_706266.2	XP_711358.2	putative ATP-dependent RNA helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly of a phased diploid Candida albicans genome facilitates allele-specific measurements and provides a simple model for repeat and indel structure.
Muzzey D, Schwartz K, Weissman JS, Sherlock G
Genome biology14(9)R97(2013)

Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes.
van het Hoog M, Rast TJ, Martchenko M, Grindle S, Dignard D, Hogues H, Cuomo C, Berriman M, Scherer S, Magee BB, Whiteway M, Chibana H, Nantel A, Magee PT
Genome biology8(4)R52(2007)

The diploid genome sequence of Candida albicans.
Jones T, Federspiel NA, Chibana H, Dungan J, Kalman S, Magee BB, Newport G, Thorstenson YR, Agabian N, Magee PT, Davis RW, Scherer S
Proceedings of the National Academy of Sciences of the United States of America101(19)7329-34(2004 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCg08023	XM_706266.2 Latest version!	Candida albicans SC5314 putative ATP-dependent RNA helicase (SPB4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPB4 ( XM_706266.2 ) cDNA ORF clone, Candida albicans SC5314 -> XP_711358.2 Candida albicans SC5314 putative ATP-dependent RNA helicase (SPB4), partial mRNA.

Price & Availability↑

CloneID	OCg08023
Clone ID Related Accession (Same CDS sequence)	XM_706266.2
Accession Version	XM_706266.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522684800000
Organism	Candida albicans SC5314
Product	putative ATP-dependent RNA helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_032093). On Dec 8, 2016 this sequence version replaced XM_706266.1. ##Genome-Annotation-Data-START## Annotation Provider :: CGD Annotation Status :: Full annotation Annotation Version :: A22-s07-m01-r01 URL :: http://www.candidagenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAATCAA ATACTGAATC TCTTGCATGG GAGAATTTAC GTTATGACTT GCATTCATGG 
ATCAAAGAAG CAATATCATC AATGGGGTAT CCCACTATGA CTCCAGTTCA AGCATCGACT 
ATCCCATTAT TGAGTGGTAA TAAAGATGTT GTAGTTGAAG CAGTCACTGG ATCAGGGAAG 
ACATTATCAT TTGTAATCCC TGTTTTACAA AAAATTTCTG ATAGATTATA TAAACCCGAT 
AGTGATGGAG ATTTACCAGA ACCAGTTAAA CGAGGACATA TGTTATCGAT TGTATTATCA 
CCAACGAGAG AATTAGCTAA TCAAATTCAA AGTGTTTTCA ATCAAGTATT ACAATATTTA 
CCTGAAGATA AGGGTACCCG TATTAACACA CAATTATTGG TTGGATCTAT AGGTAATGTT 
CGAGAAGATT TAAATCAATT TTTAACGAAT CAACCACAAA TATTAATTGG AACTCCAGGG 
AGAATCTTGG AATTTTTAGG TAGTTCACAA TCAATTAAAA CATCATCTTT GGAAATAGTT 
ATCCTTGATG AAGCTGATAA ATTATTAGAT TTCCTGTTTG AAAAAGATGT GATCAATATA 
TTGAAAAAAT TACCTAAACA ACGTCGAACA GGATTATTTT CAGCAACGAT ATCTTCTGCA 
GGTAATACTA TTTTCCGTAC AGGGATGAAT AATCCAGTTA AAGTTCAAGT GAAATCGAAA 
AATTATTTTG GTGAACAAAG TAATTCTCCC AAGAGTTTAC AATTATCATA TATGATGATT 
AATCCAGAAT TGAAAATAAC CACATTATTA ACGATATTAT CAAAATATCA ATATAAAAAG 
GCCATTGTTT ATTTCCCTAC TTGTACATCA GTGAAACATT TTTATCAAAT ATTCAATAAA 
GTTTATCAAC AAGAGTCTAG TGATGAACCT CTTAAATTTT TTTCATTACA TGGTCAACTT 
AATACTAAAT CAAGATTGAA GACATTAGAT AATTTCACTC AAGGGGATAT TAATCTTTAT 
AAGCATATTC TTATGACTAC TGATGTTGCA GCAAGAGGAA TTGATATACC TGATGTTGAT 
TTAGTGATAC AAATAGATCC ACCTACTGAC CCCAATGTTT TTTTACATCG TTGTGGTAGA 
ACTGGAAGAG CAAATAAAGT TGGTCGAGCC ATTGTTATGT TAAATGATAA TTGTCAAGAA 
GAAGATTATA TTGGATTTAT GGAAGTAAAA GGAATTTTTT TAACAAAACA AGATATACCA 
ACATCATCAG CAACAAAAAA TTTACATGAA AATTTCCAAA TTAAACTTCG ACAATATATG 
CTTGAAGATA GAGCACGACA TGAATTAGCG GTGAAATCAT ATGTGGGATT TATTCGATAT 
TATTCTAAAC ACATAGCCAG TTCGATTTTC CGATTAGCTA CATTAGATTA TTTGGGGATT 
GCCAAGATGT ATGGATTATT AAGATTACCG AAAATGCCTG AAACGAAATA TATTGATAAT 
TCGATAATGC CCACTGATGG TTGGTTAGGT GAAATTATCG ATATGGATAA ATATAGTTAT 
GCTGATAAAT TACAAGAGAA AACTCGATTA GAAAATTTAG AGAATGATAA ATTGAAAAAA 
ATTCAGGATG CAAAAAGAAG AAAAGAATTA AAAATTAAAA ATGAAGCTTG GTCAAGTAAA 
TCAGAAAGAA AAGAAAATAA ATTAGATCGT AAAGAAAAAT TGAAAAGAAA ACGTGAAGCC 
ATTGAAAAAC AAATTATGGA AGAAGAAAGA TTAAATGATG GAGGCAACAA AGATGAGGAG 
GATGAAGTTG TTGAAGATTG GAAAGATATG GTAAGAAAGA ATAAAAAAAA GCAAAAGAAA 
AATAATTCTA TACAAGGTTC ATTTGATGAT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_711358.2
CDS	1..1896
Translation	MKSNTESLAWENLRYDLHSWIKEAISSMGYPTMTPVQASTIPLLSGNKDVVVEAVTGSGKTLSFVIPVLQKISDRLYKPDSDGDLPEPVKRGHMLSIVLSPTRELANQIQSVFNQVLQYLPEDKGTRINTQLLVGSIGNVREDLNQFLTNQPQILIGTPGRILEFLGSSQSIKTSSLEIVILDEADKLLDFLFEKDVINILKKLPKQRRTGLFSATISSAGNTIFRTGMNNPVKVQVKSKNYFGEQSNSPKSLQLSYMMINPELKITTLLTILSKYQYKKAIVYFPTCTSVKHFYQIFNKVYQQESSDEPLKFFSLHGQLNTKSRLKTLDNFTQGDINLYKHILMTTDVAARGIDIPDVDLVIQIDPPTDPNVFLHRCGRTGRANKVGRAIVMLNDNCQEEDYIGFMEVKGIFLTKQDIPTSSATKNLHENFQIKLRQYMLEDRARHELAVKSYVGFIRYYSKHIASSIFRLATLDYLGIAKMYGLLRLPKMPETKYIDNSIMPTDGWLGEIIDMDKYSYADKLQEKTRLENLENDKLKKIQDAKRRKELKIKNEAWSSKSERKENKLDRKEKLKRKREAIEKQIMEEERLNDGGNKDEEDEVVEDWKDMVRKNKKKQKKNNSIQGSFDDL

Target ORF information:

RefSeq Version	XM_706266.2
Organism	Candida albicans SC5314
Definition	Candida albicans SC5314 putative ATP-dependent RNA helicase (SPB4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_706266.2

ORF Insert Sequence:

ATGAAATCAA ATACTGAATC TCTTGCATGG GAGAATTTAC GTTATGACTT GCATTCATGG 
ATCAAAGAAG CAATATCATC AATGGGGTAT CCCACTATGA CTCCAGTTCA AGCATCGACT 
ATCCCATTAT TGAGTGGTAA TAAAGATGTT GTAGTTGAAG CAGTCACTGG ATCAGGGAAG 
ACATTATCAT TTGTAATCCC TGTTTTACAA AAAATTTCTG ATAGATTATA TAAACCCGAT 
AGTGATGGAG ATTTACCAGA ACCAGTTAAA CGAGGACATA TGTTATCGAT TGTATTATCA 
CCAACGAGAG AATTAGCTAA TCAAATTCAA AGTGTTTTCA ATCAAGTATT ACAATATTTA 
CCTGAAGATA AGGGTACCCG TATTAACACA CAATTATTGG TTGGATCTAT AGGTAATGTT 
CGAGAAGATT TAAATCAATT TTTAACGAAT CAACCACAAA TATTAATTGG AACTCCAGGG 
AGAATCTTGG AATTTTTAGG TAGTTCACAA TCAATTAAAA CATCATCTTT GGAAATAGTT 
ATCCTTGATG AAGCTGATAA ATTATTAGAT TTCCTGTTTG AAAAAGATGT GATCAATATA 
TTGAAAAAAT TACCTAAACA ACGTCGAACA GGATTATTTT CAGCAACGAT ATCTTCTGCA 
GGTAATACTA TTTTCCGTAC AGGGATGAAT AATCCAGTTA AAGTTCAAGT GAAATCGAAA 
AATTATTTTG GTGAACAAAG TAATTCTCCC AAGAGTTTAC AATTATCATA TATGATGATT 
AATCCAGAAT TGAAAATAAC CACATTATTA ACGATATTAT CAAAATATCA ATATAAAAAG 
GCCATTGTTT ATTTCCCTAC TTGTACATCA GTGAAACATT TTTATCAAAT ATTCAATAAA 
GTTTATCAAC AAGAGTCTAG TGATGAACCT CTTAAATTTT TTTCATTACA TGGTCAACTT 
AATACTAAAT CAAGATTGAA GACATTAGAT AATTTCACTC AAGGGGATAT TAATCTTTAT 
AAGCATATTC TTATGACTAC TGATGTTGCA GCAAGAGGAA TTGATATACC TGATGTTGAT 
TTAGTGATAC AAATAGATCC ACCTACTGAC CCCAATGTTT TTTTACATCG TTGTGGTAGA 
ACTGGAAGAG CAAATAAAGT TGGTCGAGCC ATTGTTATGT TAAATGATAA TTGTCAAGAA 
GAAGATTATA TTGGATTTAT GGAAGTAAAA GGAATTTTTT TAACAAAACA AGATATACCA 
ACATCATCAG CAACAAAAAA TTTACATGAA AATTTCCAAA TTAAACTTCG ACAATATATG 
CTTGAAGATA GAGCACGACA TGAATTAGCG GTGAAATCAT ATGTGGGATT TATTCGATAT 
TATTCTAAAC ACATAGCCAG TTCGATTTTC CGATTAGCTA CATTAGATTA TTTGGGGATT 
GCCAAGATGT ATGGATTATT AAGATTACCG AAAATGCCTG AAACGAAATA TATTGATAAT 
TCGATAATGC CCACTGATGG TTGGTTAGGT GAAATTATCG ATATGGATAA ATATAGTTAT 
GCTGATAAAT TACAAGAGAA AACTCGATTA GAAAATTTAG AGAATGATAA ATTGAAAAAA 
ATTCAGGATG CAAAAAGAAG AAAAGAATTA AAAATTAAAA ATGAAGCTTG GTCAAGTAAA 
TCAGAAAGAA AAGAAAATAA ATTAGATCGT AAAGAAAAAT TGAAAAGAAA ACGTGAAGCC 
ATTGAAAAAC AAATTATGGA AGAAGAAAGA TTAAATGATG GAGGCAACAA AGATGAGGAG 
GATGAAGTTG TTGAAGATTG GAAAGATATG GTAAGAAAGA ATAAAAAAAA GCAAAAGAAA 
AATAATTCTA TACAAGGTTC ATTTGATGAT TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly of a phased diploid Candida albicans genome facilitates allele-specific measurements and provides a simple model for repeat and indel structure.
Genome biology14(9)R97(2013)
Muzzey D,Schwartz K,Weissman JS,Sherlock G

Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes.
Genome biology8(4)R52(2007)
van het Hoog M,Rast TJ,Martchenko M,Grindle S,Dignard D,Hogues H,Cuomo C,Berriman M,Scherer S,Magee BB,Whiteway M,Chibana H,Nantel A,Magee PT

The diploid genome sequence of Candida albicans.
Proceedings of the National Academy of Sciences of the United States of America101(19)7329-34(2004 May)
Jones T,Federspiel NA,Chibana H,Dungan J,Kalman S,Magee BB,Newport G,Thorstenson YR,Agabian N,Magee PT,Davis RW,Scherer S

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SPB4 cDNA ORF clone, Candida albicans SC5314

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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