acly-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	acly-2
Entrez Gene ID	179795
Full Name	ATP-citrate synthase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_073866.4	NP_506267.1	ATP-citrate synthase
NM_073866.3	NP_506267.1	ATP-citrate synthase

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06719	XP_003709430.1
Mus musculus (house mouse)	Acly	NP_001186225.1
Gallus gallus (chicken)	ACLY	NP_001025711.1
Danio rerio (zebrafish)	aclya	NP_001002649.1
Caenorhabditis elegans (roundworm)	acly-2	NP_506267.1
Arabidopsis thaliana (thale cress)	ACLB-2	NP_199757.1
Arabidopsis thaliana (thale cress)	ACLB-1	NP_187317.1
Caenorhabditis elegans (roundworm)	D1005.1	NP_508280.1
Rattus norvegicus (Norway rat)	Acly	NP_058683.2
Xenopus tropicalis (tropical clawed frog)	acly	NP_001008028.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010156	XP_319323.3
Oryza sativa (rice)	Os01g0300200	NP_001042817.1
Macaca mulatta (Rhesus monkey)	ACLY	XP_001108114.2
Bos taurus (cattle)	ACLY	NP_001032534.1
Homo sapiens (human)	ACLY	NP_001087.2
Pan troglodytes (chimpanzee)	ACLY	XP_511495.3
Canis lupus familiaris (dog)	ACLY	XP_850198.1
Drosophila melanogaster (fruit fly)	ATPCL	NP_725514.2
Schizosaccharomyces pombe (fission yeast)	SPBC1703.07	NP_596202.1
Neurospora crassa	NCU06785	XP_963626.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following acly-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acly-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa345678	NM_073866.4 Latest version!	Caenorhabditis elegans ATP-citrate synthase (acly-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa345678	NM_073866.3	Caenorhabditis elegans ATP-citrate synthase (acly-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

acly-2 ( NM_073866.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_506267.1 Caenorhabditis elegans ATP-citrate synthase (acly-2), mRNA.

Price & Availability↑

CloneID	OCa345678
Clone ID Related Accession (Same CDS sequence)	NM_073866.4 , NM_073866.3
Accession Version	NM_073866.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Caenorhabditis elegans
Product	ATP-citrate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_073866.3.

ATGTCGGCTA AAGCTGTCAG CGAACTCTCA GGAAAAGAGG TTTTGTACAA ATATTTCGAG 
TCGACCGGAA TTGTCAGTGC CCCACATGCT TTTCACGTGA AGGCCGGAGA TAAGTTTTCT 
GACGTCGCCG CCAAGTACGA ATGGCTTGCA CAAGACAACA AGGGTGTTAT CAAGCCGGAT 
CAGCTTATCA AGCGTCGTGG AAAGCTCGGT CTTGTTAAGA TTGGGTCTCC TAAAGAGCTG 
GAAGCTTGGT TCGGCAAGAC GGCAAACAGT TATGTGAAAG TTGGCCAAAC GGAGGGAAGA 
TTGCACACGT TTATTGTTGA GCCGTTTTGT GCTCATACTG AAAATGAAGA AATGTACATC 
GCGATCTACA GCGAACGCTG TCGCGATGTT ATTATGTTCT ATGAGCAGGG AGGAGTTGAT 
ATTGGAGACG TCGAGGAAAA GGCCAGATCA GTCCATGTTC CAGTTCAACT TGATGACAAT 
GCTATGTCAA TTTCGGAGAG AGAACTAGGC GTGCTTCTCG GGCCATGTAG TGATAAGGAT 
GATATCCGAA AGTTCGTTCG CAGCTTATAC GAAGCCTACA AAGCTCTTCA CTTCACATAC 
TTGGAGATCA ATCCGTTTGT TCTCACCAAT GGCAAGATTC ATATTTTGGA CTTGGCTGCA 
AAGCTTGACG AGACTGCCAG TTTTCTGTGC TCGGATAAAT GGAGCGGTCG GAATGCTTCT 
GCACGAATCG CTCCGACTCT GGAGTTCCCG GCACCATTTG GACGTGATTT GACTTCGGAG 
GAGCAATACA TTTCTGATAT GGATGCGAAG ACTGGAGCAT CTTTGAAGCT GACTATTCTT 
AACAGAAAGG GACGCGTATG GACCATGGTG GCTGGTGGAG GAGCATCAGT TGTGTTTACC 
GATACTGTTT GTGATCTTGG AGGATCATCT GAACTTGCTA ACTACGGAGA ATATTCTGGA 
GATCCATCGG AAGCTCAAAC TTACGAGTAC GCCAAGACTA TTTTATCCGT CATGACAGAA 
GGTGCTCCAC GTCCTGATGG TAAGGTTCTC ATCATTGGTG GATCCATTGC TAACTTCACA 
AATGTCGCAA AAACATTCGG AGGAATCGTT CGTGCGTTCG AAACTTTCAT TGATAAGCTC 
AAGGAACATA ATGTTTCCAT CTACGTTCGA CGTGGTGGTC CGAACTACCA AGAAGGACTG 
CGACGTGTCA AGGATGCTGC CACAAAACTA GAAATTCCGA TTTATGTATT TGGACCGGAA 
ACTCATATGA CTGCAATTGT CGGAGCTGCG CTCGGATTGA AGCCAATGCC AACAGTCCCG 
ACGGCTCCTC AAACCACCGG ACAATTTTTG TTGAGCCCAG AAAGAAACAC TGCAGGCACG 
GAACGTCCTC CAGCATCTCC AGCTCCGAAC ACTTCCACAA TCGAACATCC TCTCGCCAAG 
AGGCACCCTC TTCATCAAAG CTTGTTCGAG AACGACACAA AGGCTATCAT TTGGGGACAA 
CAACACAAAG CTATTCAAGG AATGCTTGAT TTCGATTTTG TTTGCAGAAG ACACTCCCCA 
TCAGTCGTCG CTTCAACCTA TCCGTTCACT GGAGACAACA AGCAAAAGTA TTATTTCGGA 
CAGAAAGAAA TCCTTATTCC TGCTTACAAA TCTATGGCAA AAGCTTTCGC GAGTCATCCA 
GATGCCACTG TCATGGTCAC GTTCGCGTCG ATGCGTTCAG TTTTTGAAAC TGTTCTGGAG 
GCTCTTCAAT TTACACAAAT CAAGGTTATC GCAATTATTG CCGAAGGAGT TCCAGAAAAT 
CAAACAAGAA AATTGCTTAA AATTGCTGAA GATAAGGGTG TTACCTTAAT TGGTCCTGCT 
ACTGTTGGAG GAATCAAGCC AGGATGTTTC AAGATCGGAA ATACCGGAGG AATGATGGAT 
AATATTCTTG CTTCGAAACT TTATCGTCCT GGAAGTGTCG CTTACGTGTC CCGCTCAGGA 
GGAATGTCGA ACGAGCTCAA CAACATTATT TCACAGAATA CCAACGGAGT TTACGAAGGA 
ATTGCTATCG GAGGGGATCG CTATCCAGGA AGTACGTACA CCGACCACGT TATGAGATAT 
CAACACGATG ATCGTGTTAA GATGATTGTC CTCCTTGGAG AAGTTGGAGG TATAGAAGAA 
TATCGTATTG TCGAGCTATT GAAAGAAAAG AAAATCACGA AGCCTCTCAT TGCTTGGTGC 
ATTGGAACAT GTGCAGATCA CATTACTTCA GAAGTTCAGT TCGGTCATGC TGGAGCTTCT 
GCAAATGGAC AAGGAGAAAC TGCTGCATGC AAAAATACAG CTCTTCGTAC TGCTGGTGCT 
TTGGTTCCAG ATTCGTTTGA CGACCTCGGA AACAAGATTC GTCAGACTTA TGAAGAATTG 
CTTCGACTTG AAATCATCGT TCCACAGCCG GAAGTTCCAC CACCTGCAGT TCCAATGGAC 
TATGCCTGGG CTCGGGAACT CGGGCTTATT CGCAAACCAG CTTCCTTCAT GACCTCTATT 
TGTGACGAAC GCGGAGAAGA ATTGAACTAT GCTGGCGTCC CAATCACCAA AGTCCTCGAG 
TCGGATATGG GAATCGGTGG AGTTCTAGGC TTGCTGTGGT TCCAGAAACG TCTCCCTCCA 
CATGCCAACA AGTTCATAGA GATCTGTTTG ATGCTCACTG CTGATCACGG ACCAGCAGTT 
TCTGGTGCTC ATAATACTAT CGTCTGTGCT CGCGCTGGAA AGGACTTGAT ATCTTCTCTC 
ACATCTGGAC TCCTCACCAT CGGTGACCGA TTCGGAGGTG CTCTCGATGG AGCTGCTCGT 
CAATTCTCTG AGGCTTTCGA TCAAGGGTGG TCTCCAAATC AGTTTGTCGG AGAAATGCGC 
AAACGTGGAA CTCATATTAT GGGTATCGGC CATCGTGTCA AGAGCATCAA TAACCCTGAT 
AAGCGTGTCG AGATTCTCAA GAGATTTGCT TTGAACAAAA AGGAGTTTGC TCAAGAAACA 
CCACTTCTCG ATTATGCTCT GGAAGTTGAG AAGATCACCA CTGCCAAGAA GCCAAACCTC 
ATTCTAAATG TTGATGGAGC TATTGCCATC ATCTTCGTCG ACATCTTGCG CAACAGTGGG 
ATGTTCACAA CAGCTGAAGC TCAAGAAGTT ATCGAGATAG GTGCTCTGAA TGGAATGTTT 
GTGCTCGGTC GCTCCATCGG ATTCATCGGA CATTATTTGG ACCAATCACG ACTCAAACAA 
GGACTCTACC GTCATCCTTG GGATGACATT TCATACATCA TGCCAGAGAG AAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_506267.1
CDS	21..3320
Translation	MSAKAVSELSGKEVLYKYFESTGIVSAPHAFHVKAGDKFSDVAAKYEWLAQDNKGVIKPDQLIKRRGKLGLVKIGSPKELEAWFGKTANSYVKVGQTEGRLHTFIVEPFCAHTENEEMYIAIYSERCRDVIMFYEQGGVDIGDVEEKARSVHVPVQLDDNAMSISERELGVLLGPCSDKDDIRKFVRSLYEAYKALHFTYLEINPFVLTNGKIHILDLAAKLDETASFLCSDKWSGRNASARIAPTLEFPAPFGRDLTSEEQYISDMDAKTGASLKLTILNRKGRVWTMVAGGGASVVFTDTVCDLGGSSELANYGEYSGDPSEAQTYEYAKTILSVMTEGAPRPDGKVLIIGGSIANFTNVAKTFGGIVRAFETFIDKLKEHNVSIYVRRGGPNYQEGLRRVKDAATKLEIPIYVFGPETHMTAIVGAALGLKPMPTVPTAPQTTGQFLLSPERNTAGTERPPASPAPNTSTIEHPLAKRHPLHQSLFENDTKAIIWGQQHKAIQGMLDFDFVCRRHSPSVVASTYPFTGDNKQKYYFGQKEILIPAYKSMAKAFASHPDATVMVTFASMRSVFETVLEALQFTQIKVIAIIAEGVPENQTRKLLKIAEDKGVTLIGPATVGGIKPGCFKIGNTGGMMDNILASKLYRPGSVAYVSRSGGMSNELNNIISQNTNGVYEGIAIGGDRYPGSTYTDHVMRYQHDDRVKMIVLLGEVGGIEEYRIVELLKEKKITKPLIAWCIGTCADHITSEVQFGHAGASANGQGETAACKNTALRTAGALVPDSFDDLGNKIRQTYEELLRLEIIVPQPEVPPPAVPMDYAWARELGLIRKPASFMTSICDERGEELNYAGVPITKVLESDMGIGGVLGLLWFQKRLPPHANKFIEICLMLTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDGAARQFSEAFDQGWSPNQFVGEMRKRGTHIMGIGHRVKSINNPDKRVEILKRFALNKKEFAQETPLLDYALEVEKITTAKKPNLILNVDGAIAIIFVDILRNSGMFTTAEAQEVIEIGALNGMFVLGRSIGFIGHYLDQSRLKQGLYRHPWDDISYIMPERNL

Target ORF information:

RefSeq Version	NM_073866.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ATP-citrate synthase (acly-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_073866.4

ORF Insert Sequence:

ATGTCGGCTA AAGCTGTCAG CGAACTCTCA GGAAAAGAGG TTTTGTACAA ATATTTCGAG 
TCGACCGGAA TTGTCAGTGC CCCACATGCT TTTCACGTGA AGGCCGGAGA TAAGTTTTCT 
GACGTCGCCG CCAAGTACGA ATGGCTTGCA CAAGACAACA AGGGTGTTAT CAAGCCGGAT 
CAGCTTATCA AGCGTCGTGG AAAGCTCGGT CTTGTTAAGA TTGGGTCTCC TAAAGAGCTG 
GAAGCTTGGT TCGGCAAGAC GGCAAACAGT TATGTGAAAG TTGGCCAAAC GGAGGGAAGA 
TTGCACACGT TTATTGTTGA GCCGTTTTGT GCTCATACTG AAAATGAAGA AATGTACATC 
GCGATCTACA GCGAACGCTG TCGCGATGTT ATTATGTTCT ATGAGCAGGG AGGAGTTGAT 
ATTGGAGACG TCGAGGAAAA GGCCAGATCA GTCCATGTTC CAGTTCAACT TGATGACAAT 
GCTATGTCAA TTTCGGAGAG AGAACTAGGC GTGCTTCTCG GGCCATGTAG TGATAAGGAT 
GATATCCGAA AGTTCGTTCG CAGCTTATAC GAAGCCTACA AAGCTCTTCA CTTCACATAC 
TTGGAGATCA ATCCGTTTGT TCTCACCAAT GGCAAGATTC ATATTTTGGA CTTGGCTGCA 
AAGCTTGACG AGACTGCCAG TTTTCTGTGC TCGGATAAAT GGAGCGGTCG GAATGCTTCT 
GCACGAATCG CTCCGACTCT GGAGTTCCCG GCACCATTTG GACGTGATTT GACTTCGGAG 
GAGCAATACA TTTCTGATAT GGATGCGAAG ACTGGAGCAT CTTTGAAGCT GACTATTCTT 
AACAGAAAGG GACGCGTATG GACCATGGTG GCTGGTGGAG GAGCATCAGT TGTGTTTACC 
GATACTGTTT GTGATCTTGG AGGATCATCT GAACTTGCTA ACTACGGAGA ATATTCTGGA 
GATCCATCGG AAGCTCAAAC TTACGAGTAC GCCAAGACTA TTTTATCCGT CATGACAGAA 
GGTGCTCCAC GTCCTGATGG TAAGGTTCTC ATCATTGGTG GATCCATTGC TAACTTCACA 
AATGTCGCAA AAACATTCGG AGGAATCGTT CGTGCGTTCG AAACTTTCAT TGATAAGCTC 
AAGGAACATA ATGTTTCCAT CTACGTTCGA CGTGGTGGTC CGAACTACCA AGAAGGACTG 
CGACGTGTCA AGGATGCTGC CACAAAACTA GAAATTCCGA TTTATGTATT TGGACCGGAA 
ACTCATATGA CTGCAATTGT CGGAGCTGCG CTCGGATTGA AGCCAATGCC AACAGTCCCG 
ACGGCTCCTC AAACCACCGG ACAATTTTTG TTGAGCCCAG AAAGAAACAC TGCAGGCACG 
GAACGTCCTC CAGCATCTCC AGCTCCGAAC ACTTCCACAA TCGAACATCC TCTCGCCAAG 
AGGCACCCTC TTCATCAAAG CTTGTTCGAG AACGACACAA AGGCTATCAT TTGGGGACAA 
CAACACAAAG CTATTCAAGG AATGCTTGAT TTCGATTTTG TTTGCAGAAG ACACTCCCCA 
TCAGTCGTCG CTTCAACCTA TCCGTTCACT GGAGACAACA AGCAAAAGTA TTATTTCGGA 
CAGAAAGAAA TCCTTATTCC TGCTTACAAA TCTATGGCAA AAGCTTTCGC GAGTCATCCA 
GATGCCACTG TCATGGTCAC GTTCGCGTCG ATGCGTTCAG TTTTTGAAAC TGTTCTGGAG 
GCTCTTCAAT TTACACAAAT CAAGGTTATC GCAATTATTG CCGAAGGAGT TCCAGAAAAT 
CAAACAAGAA AATTGCTTAA AATTGCTGAA GATAAGGGTG TTACCTTAAT TGGTCCTGCT 
ACTGTTGGAG GAATCAAGCC AGGATGTTTC AAGATCGGAA ATACCGGAGG AATGATGGAT 
AATATTCTTG CTTCGAAACT TTATCGTCCT GGAAGTGTCG CTTACGTGTC CCGCTCAGGA 
GGAATGTCGA ACGAGCTCAA CAACATTATT TCACAGAATA CCAACGGAGT TTACGAAGGA 
ATTGCTATCG GAGGGGATCG CTATCCAGGA AGTACGTACA CCGACCACGT TATGAGATAT 
CAACACGATG ATCGTGTTAA GATGATTGTC CTCCTTGGAG AAGTTGGAGG TATAGAAGAA 
TATCGTATTG TCGAGCTATT GAAAGAAAAG AAAATCACGA AGCCTCTCAT TGCTTGGTGC 
ATTGGAACAT GTGCAGATCA CATTACTTCA GAAGTTCAGT TCGGTCATGC TGGAGCTTCT 
GCAAATGGAC AAGGAGAAAC TGCTGCATGC AAAAATACAG CTCTTCGTAC TGCTGGTGCT 
TTGGTTCCAG ATTCGTTTGA CGACCTCGGA AACAAGATTC GTCAGACTTA TGAAGAATTG 
CTTCGACTTG AAATCATCGT TCCACAGCCG GAAGTTCCAC CACCTGCAGT TCCAATGGAC 
TATGCCTGGG CTCGGGAACT CGGGCTTATT CGCAAACCAG CTTCCTTCAT GACCTCTATT 
TGTGACGAAC GCGGAGAAGA ATTGAACTAT GCTGGCGTCC CAATCACCAA AGTCCTCGAG 
TCGGATATGG GAATCGGTGG AGTTCTAGGC TTGCTGTGGT TCCAGAAACG TCTCCCTCCA 
CATGCCAACA AGTTCATAGA GATCTGTTTG ATGCTCACTG CTGATCACGG ACCAGCAGTT 
TCTGGTGCTC ATAATACTAT CGTCTGTGCT CGCGCTGGAA AGGACTTGAT ATCTTCTCTC 
ACATCTGGAC TCCTCACCAT CGGTGACCGA TTCGGAGGTG CTCTCGATGG AGCTGCTCGT 
CAATTCTCTG AGGCTTTCGA TCAAGGGTGG TCTCCAAATC AGTTTGTCGG AGAAATGCGC 
AAACGTGGAA CTCATATTAT GGGTATCGGC CATCGTGTCA AGAGCATCAA TAACCCTGAT 
AAGCGTGTCG AGATTCTCAA GAGATTTGCT TTGAACAAAA AGGAGTTTGC TCAAGAAACA 
CCACTTCTCG ATTATGCTCT GGAAGTTGAG AAGATCACCA CTGCCAAGAA GCCAAACCTC 
ATTCTAAATG TTGATGGAGC TATTGCCATC ATCTTCGTCG ACATCTTGCG CAACAGTGGG 
ATGTTCACAA CAGCTGAAGC TCAAGAAGTT ATCGAGATAG GTGCTCTGAA TGGAATGTTT 
GTGCTCGGTC GCTCCATCGG ATTCATCGGA CATTATTTGG ACCAATCACG ACTCAAACAA 
GGACTCTACC GTCATCCTTG GGATGACATT TCATACATCA TGCCAGAGAG AAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acly-2 ( NM_073866.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_506267.1 Caenorhabditis elegans ATP-citrate synthase (acly-2), partial mRNA.

Price & Availability↑

CloneID	OCa345678
Clone ID Related Accession (Same CDS sequence)	NM_073866.4 , NM_073866.3
Accession Version	NM_073866.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554652800000
Organism	Caenorhabditis elegans
Product	ATP-citrate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_073866.2. COMPLETENESS: incomplete on both ends.

ATGTCGGCTA AAGCTGTCAG CGAACTCTCA GGAAAAGAGG TTTTGTACAA ATATTTCGAG 
TCGACCGGAA TTGTCAGTGC CCCACATGCT TTTCACGTGA AGGCCGGAGA TAAGTTTTCT 
GACGTCGCCG CCAAGTACGA ATGGCTTGCA CAAGACAACA AGGGTGTTAT CAAGCCGGAT 
CAGCTTATCA AGCGTCGTGG AAAGCTCGGT CTTGTTAAGA TTGGGTCTCC TAAAGAGCTG 
GAAGCTTGGT TCGGCAAGAC GGCAAACAGT TATGTGAAAG TTGGCCAAAC GGAGGGAAGA 
TTGCACACGT TTATTGTTGA GCCGTTTTGT GCTCATACTG AAAATGAAGA AATGTACATC 
GCGATCTACA GCGAACGCTG TCGCGATGTT ATTATGTTCT ATGAGCAGGG AGGAGTTGAT 
ATTGGAGACG TCGAGGAAAA GGCCAGATCA GTCCATGTTC CAGTTCAACT TGATGACAAT 
GCTATGTCAA TTTCGGAGAG AGAACTAGGC GTGCTTCTCG GGCCATGTAG TGATAAGGAT 
GATATCCGAA AGTTCGTTCG CAGCTTATAC GAAGCCTACA AAGCTCTTCA CTTCACATAC 
TTGGAGATCA ATCCGTTTGT TCTCACCAAT GGCAAGATTC ATATTTTGGA CTTGGCTGCA 
AAGCTTGACG AGACTGCCAG TTTTCTGTGC TCGGATAAAT GGAGCGGTCG GAATGCTTCT 
GCACGAATCG CTCCGACTCT GGAGTTCCCG GCACCATTTG GACGTGATTT GACTTCGGAG 
GAGCAATACA TTTCTGATAT GGATGCGAAG ACTGGAGCAT CTTTGAAGCT GACTATTCTT 
AACAGAAAGG GACGCGTATG GACCATGGTG GCTGGTGGAG GAGCATCAGT TGTGTTTACC 
GATACTGTTT GTGATCTTGG AGGATCATCT GAACTTGCTA ACTACGGAGA ATATTCTGGA 
GATCCATCGG AAGCTCAAAC TTACGAGTAC GCCAAGACTA TTTTATCCGT CATGACAGAA 
GGTGCTCCAC GTCCTGATGG TAAGGTTCTC ATCATTGGTG GATCCATTGC TAACTTCACA 
AATGTCGCAA AAACATTCGG AGGAATCGTT CGTGCGTTCG AAACTTTCAT TGATAAGCTC 
AAGGAACATA ATGTTTCCAT CTACGTTCGA CGTGGTGGTC CGAACTACCA AGAAGGACTG 
CGACGTGTCA AGGATGCTGC CACAAAACTA GAAATTCCGA TTTATGTATT TGGACCGGAA 
ACTCATATGA CTGCAATTGT CGGAGCTGCG CTCGGATTGA AGCCAATGCC AACAGTCCCG 
ACGGCTCCTC AAACCACCGG ACAATTTTTG TTGAGCCCAG AAAGAAACAC TGCAGGCACG 
GAACGTCCTC CAGCATCTCC AGCTCCGAAC ACTTCCACAA TCGAACATCC TCTCGCCAAG 
AGGCACCCTC TTCATCAAAG CTTGTTCGAG AACGACACAA AGGCTATCAT TTGGGGACAA 
CAACACAAAG CTATTCAAGG AATGCTTGAT TTCGATTTTG TTTGCAGAAG ACACTCCCCA 
TCAGTCGTCG CTTCAACCTA TCCGTTCACT GGAGACAACA AGCAAAAGTA TTATTTCGGA 
CAGAAAGAAA TCCTTATTCC TGCTTACAAA TCTATGGCAA AAGCTTTCGC GAGTCATCCA 
GATGCCACTG TCATGGTCAC GTTCGCGTCG ATGCGTTCAG TTTTTGAAAC TGTTCTGGAG 
GCTCTTCAAT TTACACAAAT CAAGGTTATC GCAATTATTG CCGAAGGAGT TCCAGAAAAT 
CAAACAAGAA AATTGCTTAA AATTGCTGAA GATAAGGGTG TTACCTTAAT TGGTCCTGCT 
ACTGTTGGAG GAATCAAGCC AGGATGTTTC AAGATCGGAA ATACCGGAGG AATGATGGAT 
AATATTCTTG CTTCGAAACT TTATCGTCCT GGAAGTGTCG CTTACGTGTC CCGCTCAGGA 
GGAATGTCGA ACGAGCTCAA CAACATTATT TCACAGAATA CCAACGGAGT TTACGAAGGA 
ATTGCTATCG GAGGGGATCG CTATCCAGGA AGTACGTACA CCGACCACGT TATGAGATAT 
CAACACGATG ATCGTGTTAA GATGATTGTC CTCCTTGGAG AAGTTGGAGG TATAGAAGAA 
TATCGTATTG TCGAGCTATT GAAAGAAAAG AAAATCACGA AGCCTCTCAT TGCTTGGTGC 
ATTGGAACAT GTGCAGATCA CATTACTTCA GAAGTTCAGT TCGGTCATGC TGGAGCTTCT 
GCAAATGGAC AAGGAGAAAC TGCTGCATGC AAAAATACAG CTCTTCGTAC TGCTGGTGCT 
TTGGTTCCAG ATTCGTTTGA CGACCTCGGA AACAAGATTC GTCAGACTTA TGAAGAATTG 
CTTCGACTTG AAATCATCGT TCCACAGCCG GAAGTTCCAC CACCTGCAGT TCCAATGGAC 
TATGCCTGGG CTCGGGAACT CGGGCTTATT CGCAAACCAG CTTCCTTCAT GACCTCTATT 
TGTGACGAAC GCGGAGAAGA ATTGAACTAT GCTGGCGTCC CAATCACCAA AGTCCTCGAG 
TCGGATATGG GAATCGGTGG AGTTCTAGGC TTGCTGTGGT TCCAGAAACG TCTCCCTCCA 
CATGCCAACA AGTTCATAGA GATCTGTTTG ATGCTCACTG CTGATCACGG ACCAGCAGTT 
TCTGGTGCTC ATAATACTAT CGTCTGTGCT CGCGCTGGAA AGGACTTGAT ATCTTCTCTC 
ACATCTGGAC TCCTCACCAT CGGTGACCGA TTCGGAGGTG CTCTCGATGG AGCTGCTCGT 
CAATTCTCTG AGGCTTTCGA TCAAGGGTGG TCTCCAAATC AGTTTGTCGG AGAAATGCGC 
AAACGTGGAA CTCATATTAT GGGTATCGGC CATCGTGTCA AGAGCATCAA TAACCCTGAT 
AAGCGTGTCG AGATTCTCAA GAGATTTGCT TTGAACAAAA AGGAGTTTGC TCAAGAAACA 
CCACTTCTCG ATTATGCTCT GGAAGTTGAG AAGATCACCA CTGCCAAGAA GCCAAACCTC 
ATTCTAAATG TTGATGGAGC TATTGCCATC ATCTTCGTCG ACATCTTGCG CAACAGTGGG 
ATGTTCACAA CAGCTGAAGC TCAAGAAGTT ATCGAGATAG GTGCTCTGAA TGGAATGTTT 
GTGCTCGGTC GCTCCATCGG ATTCATCGGA CATTATTTGG ACCAATCACG ACTCAAACAA 
GGACTCTACC GTCATCCTTG GGATGACATT TCATACATCA TGCCAGAGAG AAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_506267.1
CDS	1..3300
Translation	MSAKAVSELSGKEVLYKYFESTGIVSAPHAFHVKAGDKFSDVAAKYEWLAQDNKGVIKPDQLIKRRGKLGLVKIGSPKELEAWFGKTANSYVKVGQTEGRLHTFIVEPFCAHTENEEMYIAIYSERCRDVIMFYEQGGVDIGDVEEKARSVHVPVQLDDNAMSISERELGVLLGPCSDKDDIRKFVRSLYEAYKALHFTYLEINPFVLTNGKIHILDLAAKLDETASFLCSDKWSGRNASARIAPTLEFPAPFGRDLTSEEQYISDMDAKTGASLKLTILNRKGRVWTMVAGGGASVVFTDTVCDLGGSSELANYGEYSGDPSEAQTYEYAKTILSVMTEGAPRPDGKVLIIGGSIANFTNVAKTFGGIVRAFETFIDKLKEHNVSIYVRRGGPNYQEGLRRVKDAATKLEIPIYVFGPETHMTAIVGAALGLKPMPTVPTAPQTTGQFLLSPERNTAGTERPPASPAPNTSTIEHPLAKRHPLHQSLFENDTKAIIWGQQHKAIQGMLDFDFVCRRHSPSVVASTYPFTGDNKQKYYFGQKEILIPAYKSMAKAFASHPDATVMVTFASMRSVFETVLEALQFTQIKVIAIIAEGVPENQTRKLLKIAEDKGVTLIGPATVGGIKPGCFKIGNTGGMMDNILASKLYRPGSVAYVSRSGGMSNELNNIISQNTNGVYEGIAIGGDRYPGSTYTDHVMRYQHDDRVKMIVLLGEVGGIEEYRIVELLKEKKITKPLIAWCIGTCADHITSEVQFGHAGASANGQGETAACKNTALRTAGALVPDSFDDLGNKIRQTYEELLRLEIIVPQPEVPPPAVPMDYAWARELGLIRKPASFMTSICDERGEELNYAGVPITKVLESDMGIGGVLGLLWFQKRLPPHANKFIEICLMLTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDGAARQFSEAFDQGWSPNQFVGEMRKRGTHIMGIGHRVKSINNPDKRVEILKRFALNKKEFAQETPLLDYALEVEKITTAKKPNLILNVDGAIAIIFVDILRNSGMFTTAEAQEVIEIGALNGMFVLGRSIGFIGHYLDQSRLKQGLYRHPWDDISYIMPERNL

Target ORF information:

RefSeq Version	NM_073866.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ATP-citrate synthase (acly-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_073866.3

ORF Insert Sequence:

ATGTCGGCTA AAGCTGTCAG CGAACTCTCA GGAAAAGAGG TTTTGTACAA ATATTTCGAG 
TCGACCGGAA TTGTCAGTGC CCCACATGCT TTTCACGTGA AGGCCGGAGA TAAGTTTTCT 
GACGTCGCCG CCAAGTACGA ATGGCTTGCA CAAGACAACA AGGGTGTTAT CAAGCCGGAT 
CAGCTTATCA AGCGTCGTGG AAAGCTCGGT CTTGTTAAGA TTGGGTCTCC TAAAGAGCTG 
GAAGCTTGGT TCGGCAAGAC GGCAAACAGT TATGTGAAAG TTGGCCAAAC GGAGGGAAGA 
TTGCACACGT TTATTGTTGA GCCGTTTTGT GCTCATACTG AAAATGAAGA AATGTACATC 
GCGATCTACA GCGAACGCTG TCGCGATGTT ATTATGTTCT ATGAGCAGGG AGGAGTTGAT 
ATTGGAGACG TCGAGGAAAA GGCCAGATCA GTCCATGTTC CAGTTCAACT TGATGACAAT 
GCTATGTCAA TTTCGGAGAG AGAACTAGGC GTGCTTCTCG GGCCATGTAG TGATAAGGAT 
GATATCCGAA AGTTCGTTCG CAGCTTATAC GAAGCCTACA AAGCTCTTCA CTTCACATAC 
TTGGAGATCA ATCCGTTTGT TCTCACCAAT GGCAAGATTC ATATTTTGGA CTTGGCTGCA 
AAGCTTGACG AGACTGCCAG TTTTCTGTGC TCGGATAAAT GGAGCGGTCG GAATGCTTCT 
GCACGAATCG CTCCGACTCT GGAGTTCCCG GCACCATTTG GACGTGATTT GACTTCGGAG 
GAGCAATACA TTTCTGATAT GGATGCGAAG ACTGGAGCAT CTTTGAAGCT GACTATTCTT 
AACAGAAAGG GACGCGTATG GACCATGGTG GCTGGTGGAG GAGCATCAGT TGTGTTTACC 
GATACTGTTT GTGATCTTGG AGGATCATCT GAACTTGCTA ACTACGGAGA ATATTCTGGA 
GATCCATCGG AAGCTCAAAC TTACGAGTAC GCCAAGACTA TTTTATCCGT CATGACAGAA 
GGTGCTCCAC GTCCTGATGG TAAGGTTCTC ATCATTGGTG GATCCATTGC TAACTTCACA 
AATGTCGCAA AAACATTCGG AGGAATCGTT CGTGCGTTCG AAACTTTCAT TGATAAGCTC 
AAGGAACATA ATGTTTCCAT CTACGTTCGA CGTGGTGGTC CGAACTACCA AGAAGGACTG 
CGACGTGTCA AGGATGCTGC CACAAAACTA GAAATTCCGA TTTATGTATT TGGACCGGAA 
ACTCATATGA CTGCAATTGT CGGAGCTGCG CTCGGATTGA AGCCAATGCC AACAGTCCCG 
ACGGCTCCTC AAACCACCGG ACAATTTTTG TTGAGCCCAG AAAGAAACAC TGCAGGCACG 
GAACGTCCTC CAGCATCTCC AGCTCCGAAC ACTTCCACAA TCGAACATCC TCTCGCCAAG 
AGGCACCCTC TTCATCAAAG CTTGTTCGAG AACGACACAA AGGCTATCAT TTGGGGACAA 
CAACACAAAG CTATTCAAGG AATGCTTGAT TTCGATTTTG TTTGCAGAAG ACACTCCCCA 
TCAGTCGTCG CTTCAACCTA TCCGTTCACT GGAGACAACA AGCAAAAGTA TTATTTCGGA 
CAGAAAGAAA TCCTTATTCC TGCTTACAAA TCTATGGCAA AAGCTTTCGC GAGTCATCCA 
GATGCCACTG TCATGGTCAC GTTCGCGTCG ATGCGTTCAG TTTTTGAAAC TGTTCTGGAG 
GCTCTTCAAT TTACACAAAT CAAGGTTATC GCAATTATTG CCGAAGGAGT TCCAGAAAAT 
CAAACAAGAA AATTGCTTAA AATTGCTGAA GATAAGGGTG TTACCTTAAT TGGTCCTGCT 
ACTGTTGGAG GAATCAAGCC AGGATGTTTC AAGATCGGAA ATACCGGAGG AATGATGGAT 
AATATTCTTG CTTCGAAACT TTATCGTCCT GGAAGTGTCG CTTACGTGTC CCGCTCAGGA 
GGAATGTCGA ACGAGCTCAA CAACATTATT TCACAGAATA CCAACGGAGT TTACGAAGGA 
ATTGCTATCG GAGGGGATCG CTATCCAGGA AGTACGTACA CCGACCACGT TATGAGATAT 
CAACACGATG ATCGTGTTAA GATGATTGTC CTCCTTGGAG AAGTTGGAGG TATAGAAGAA 
TATCGTATTG TCGAGCTATT GAAAGAAAAG AAAATCACGA AGCCTCTCAT TGCTTGGTGC 
ATTGGAACAT GTGCAGATCA CATTACTTCA GAAGTTCAGT TCGGTCATGC TGGAGCTTCT 
GCAAATGGAC AAGGAGAAAC TGCTGCATGC AAAAATACAG CTCTTCGTAC TGCTGGTGCT 
TTGGTTCCAG ATTCGTTTGA CGACCTCGGA AACAAGATTC GTCAGACTTA TGAAGAATTG 
CTTCGACTTG AAATCATCGT TCCACAGCCG GAAGTTCCAC CACCTGCAGT TCCAATGGAC 
TATGCCTGGG CTCGGGAACT CGGGCTTATT CGCAAACCAG CTTCCTTCAT GACCTCTATT 
TGTGACGAAC GCGGAGAAGA ATTGAACTAT GCTGGCGTCC CAATCACCAA AGTCCTCGAG 
TCGGATATGG GAATCGGTGG AGTTCTAGGC TTGCTGTGGT TCCAGAAACG TCTCCCTCCA 
CATGCCAACA AGTTCATAGA GATCTGTTTG ATGCTCACTG CTGATCACGG ACCAGCAGTT 
TCTGGTGCTC ATAATACTAT CGTCTGTGCT CGCGCTGGAA AGGACTTGAT ATCTTCTCTC 
ACATCTGGAC TCCTCACCAT CGGTGACCGA TTCGGAGGTG CTCTCGATGG AGCTGCTCGT 
CAATTCTCTG AGGCTTTCGA TCAAGGGTGG TCTCCAAATC AGTTTGTCGG AGAAATGCGC 
AAACGTGGAA CTCATATTAT GGGTATCGGC CATCGTGTCA AGAGCATCAA TAACCCTGAT 
AAGCGTGTCG AGATTCTCAA GAGATTTGCT TTGAACAAAA AGGAGTTTGC TCAAGAAACA 
CCACTTCTCG ATTATGCTCT GGAAGTTGAG AAGATCACCA CTGCCAAGAA GCCAAACCTC 
ATTCTAAATG TTGATGGAGC TATTGCCATC ATCTTCGTCG ACATCTTGCG CAACAGTGGG 
ATGTTCACAA CAGCTGAAGC TCAAGAAGTT ATCGAGATAG GTGCTCTGAA TGGAATGTTT 
GTGCTCGGTC GCTCCATCGG ATTCATCGGA CATTATTTGG ACCAATCACG ACTCAAACAA 
GGACTCTACC GTCATCCTTG GGATGACATT TCATACATCA TGCCAGAGAG AAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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acly-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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