spt-16 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	spt-16
Entrez Gene ID	172984
Full Name	FACT complex subunit spt-16
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_060420.4	NP_492821.1	FACT complex subunit spt-16
NM_060420.5	NP_492821.1	FACT complex subunit spt-16

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F09889g	XP_455530.1
Mus musculus (house mouse)	Supt16	NP_291096.2
Schizosaccharomyces pombe (fission yeast)	spt16	NP_596526.1
Eremothecium gossypii	AGOS_AER360C	NP_985216.2
Oryza sativa (rice)	Os04g0321600	NP_001052463.1
Pan troglodytes (chimpanzee)	SUPT16H	XP_528703.2
Rattus norvegicus (Norway rat)	Supt16h	NP_001100731.1
Danio rerio (zebrafish)	supt16h	NP_001091053.1
Bos taurus (cattle)	SUPT16H	NP_001179321.1
Arabidopsis thaliana (thale cress)	SPT16	NP_192809.1
Neurospora crassa	NCU01164	XP_961530.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006817	XP_308929.4
Homo sapiens (human)	SUPT16H	NP_009123.1
Canis lupus familiaris (dog)	SUPT16H	XP_851566.1
Saccharomyces cerevisiae (baker's yeast)	SPT16	NP_011308.1
Magnaporthe oryzae (rice blast fungus)	MGG_07316	XP_003715548.1
Drosophila melanogaster (fruit fly)	dre4	NP_728686.2
Caenorhabditis elegans (roundworm)	F55A3.3	NP_492821.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following spt-16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spt-16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336658	NM_060420.5 Latest version!	Caenorhabditis elegans FACT complex subunit spt-16 (spt-16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa336658	NM_060420.4	Caenorhabditis elegans FACT complex subunit spt-16 (spt-16), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spt-16 ( NM_060420.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492821.1 Caenorhabditis elegans FACT complex subunit spt-16 (spt-16), partial mRNA.

Price & Availability↑

CloneID	OCa336658
Clone ID Related Accession (Same CDS sequence)	NM_060420.4 , NM_060420.5
Accession Version	NM_060420.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554652800000
Organism	Caenorhabditis elegans
Product	FACT complex subunit spt-16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_060420.3. COMPLETENESS: incomplete on both ends.

ATGTCTGGAA AACGGGCAGT TCTCAACAAG GATCTCTTTT TCCAGAGAGC GGAGAGGCTT 
TATGAGCACT GGGAGAAGGG AGCCGATGGT CTAGATTCGA TCAAATCACT TGCTTTTGTA 
TACGGAGAGA CTGATAATCC ATATACAAAG ACCAGCGCAC TATTTACCTG GCTGTTTGGC 
CACGAAATCG CTGACACAGT CCTGTTGCTT CTCAAAGATC ACATTTACAT TCTCGGAAGT 
AATCGCAAAG TCGAATTCTT CGGATCAGTC ACTGGAGACA ATCAGAGCTC AGGAAAAGTG 
CCAACAGTCA GCACACTGCT CCGCGATAAA ACCGACAAAG ACGCTGGAAA CTTTGAAAAG 
CTCATCGATC ACATCAAAAG TGCCGGCGGA GATGTTGGAA ATTTCGTCAA AGAGAAATTC 
AGCTCGGAAT TCGTCAGTTC GTGGAATAAG GCGCTCGAGG AAGGCGGTGT CAATAAGAAT 
GACGTCACTC TCGCGTTTAC TCATCTGTTC GCAGTGAAAG ACGATAAGGA GATGGATTTG 
ATTCGAAAAT CAGCACAAGC TACAACCGCA TCATGGACAG CTGCAAGAGC TCGCTACGTA 
GAGATCATTG ATCAAGAAAA GCGAGTCCGT CACTCTGTTC TCTCCAATGA GTTTGCCGCC 
TTCATGAAGG ATTCAAAAGT ACAACAGGCA CTGGCCAAGT ATGAAGCCGA CACATGCTAT 
GATCCAATCG TAATGTCCGG TGGAAATTAC TCGTTCAAAT GGAATCATGA AAGCTCGGAA 
TCTCATCTTC ACAGCCAGTT TGGAACGATC ATCACTTCGT TCGGAGCTCG TTTATCAGAA 
TATTGCACAA ATTTGACGCG AACTATGCTC ATCTTCCCAT CTTCTGAACT TGAGACTGCC 
TATGAAGCGA TTCTTGCCGC CGAGCTGGCA GTAATTGCTG CTCTGAAGCC AGGAGCCAAG 
CTGAGCGATG TTTATAAAAT TGGAATCGAT ACATTGACGG AGAAAAGTCC AAAACTTGCT 
GAAACTCTGA ACAAGAAAGA GCTTGGATTT GCGACTGGAA TCGAATTCCG TGAGAGTCGT 
CTTGCTATCA GTGCGAAGTG TGACGAGGTT GTCAAAGCAG GAATGGTCTT CATCGTTTAT 
ATTGGTGTCG ATAGTATTCC AAACAAAAAT AAAGGAGAAA AAGGAAAACC CGCCGCAATT 
GCAATCTCGG ATACAATTTT GGTTAAAGAA GAAGGCGATA ACGAGATTCT CACCGAAAAA 
GCCAAATCAC GCCTGAAGTC CAACGTCATC AAATTCAAAG AGGAGCAGGA AAACCGTGAA 
GCTGAAAAGG ACAATGATCA GAAGAAAATG CTCGGAAGAG GTCAGAGAAG TGTCGTTCTA 
ACTGATCAGA CGAGAAATAA GACGACAAAC GAAGAGTTAC GTAAGGAACG TCAGAAAGAG 
CTCGGAGTTC AGTTGAATGA GCTCGCAAAA GCTCGTCTTT CAAAGCAAGG CGGTGGGACC 
GATGAGAAAA AGTCGAAGAA ATCGAATGTG TCATATAAAA CCGAAGAGAG ATTCCCACAA 
GATGCAGACG TTCAGAAGAT GTTGATTTTC GTGGATCGCA AGTATGATTC TGTGGTTGTT 
CCAATATTTG GAATTCCTGT CCCATTCCAT ATCTCCATGA TAAAGAATTG TTCGCAATCA 
GTTGAAGGAG ATTTCACATA TCTTCGAATC AACTTTGCCA CTCCAGGATC TCAAGTTGGA 
AAGGATAGTG GGCAATTCCC ACATCCACTT GCACACTATA TGAAGGAGCT CACGTTCCGT 
GCGAGTAACA TCAAGGATCA TCACAGTGAT TCGACTGCGC CGAGCCATAA TTTGAGCACC 
GCATTCCGAC TCATCAAGGA AATGCAGAAG AGATTCAAGA CTGAAGAGGC TGAAGAGCGG 
GAGAAGGAAG GAGCTGTGAA GCAGGACAAA TTGATTTTGA GCCAGAACAA ATTGAATCCG 
AAGCTCAAGG ATCTTCTGAT TCGTCCGAAT ATTATTCAAA AGCGTATCAC TGGATCGTTG 
GAGGCCCACA CTAATGGATT TAGATACACA TCTCTTCGTG GTGATCGTAT TGATGTTCTC 
TACAACAACA TCAAACATGC ATTCTTCCAA CCGTGCGACA ATGAAATGAT CATCCTTCTG 
CACTTCCATC TCAAGAATCC AGTATTGTGG GGAAAGAAGA AGTATAAGGA TGTTCAATTC 
TACACAGAAG TCGGAGAAAT CACTACGGAT CTTGGAAAGT ATCATCATAT GCAAGATCGT 
GATGATATGC AGAGTGAACA GCAAGAACGT GAAATGCGTC GTCGTCTCAA TGCTGCATTT 
AATAGTTTCT GCGAGAAGGT GTCTCGTCTC ACAAATGATC AATTTGAATT CGATTCTCCG 
TTCGCTGGAC TCGGATTCTT TGGAGTTCCG TATCGTTCGG CTACCACACT CAAACCAACT 
GCATCGTGCC TTGTTAATCT CACCGAATGG CCTACATTCA TCGTCACACT CTCCGAAGTT 
GAACTTGTCC ACTTTGAACG TGTCTCTCTT CAACTCAAGA ATTTCGATAT GGTCTTCATC 
TTCAAAGATT ACAAGATCAA ACCTCAGATG GTCGCCCAAA TCCCGATGAG CAGCATCGAT 
AAGATAAAAG AATGGCTTCA TACTTGTGAT ATTTGGTACA GTGAAGGAAT TCAATCACTG 
AATTGGGCAA AAGTTATGAA GACAATCACT GATGATCTTG AAGCATTCTT TGAAGAAGGT 
GGATGGAGTT TCCTTAATGT TGAATCTGAT AATGAAGAGG CAATGGATGA TTCTGATGAT 
TCGGATGCTT ATGATCCTGA AGAAGAAGAT GCATCTGCTG GATCAGGAAG TGAAAGTGAT 
GAAGATGAAA GTGAAGGTGA AGAGACTGAA TCCGATGATG ATGATGAAGG ATCGTTGGAT 
TCCGATGAAT CAGAAGGAAA AGATTGGTCA GATTTGGAGG AAGAAGCAGC GAATGCTGAT 
AAGAGACGTG AAGTCGAAGA ACCTTCACGT GATCGTGATC GTAAGAGACC ACATTCGAGC 
AAGTCTGGAC CATCGCACAA ACGCAGAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492821.1
CDS	1..3093
Translation	MSGKRAVLNKDLFFQRAERLYEHWEKGADGLDSIKSLAFVYGETDNPYTKTSALFTWLFGHEIADTVLLLLKDHIYILGSNRKVEFFGSVTGDNQSSGKVPTVSTLLRDKTDKDAGNFEKLIDHIKSAGGDVGNFVKEKFSSEFVSSWNKALEEGGVNKNDVTLAFTHLFAVKDDKEMDLIRKSAQATTASWTAARARYVEIIDQEKRVRHSVLSNEFAAFMKDSKVQQALAKYEADTCYDPIVMSGGNYSFKWNHESSESHLHSQFGTIITSFGARLSEYCTNLTRTMLIFPSSELETAYEAILAAELAVIAALKPGAKLSDVYKIGIDTLTEKSPKLAETLNKKELGFATGIEFRESRLAISAKCDEVVKAGMVFIVYIGVDSIPNKNKGEKGKPAAIAISDTILVKEEGDNEILTEKAKSRLKSNVIKFKEEQENREAEKDNDQKKMLGRGQRSVVLTDQTRNKTTNEELRKERQKELGVQLNELAKARLSKQGGGTDEKKSKKSNVSYKTEERFPQDADVQKMLIFVDRKYDSVVVPIFGIPVPFHISMIKNCSQSVEGDFTYLRINFATPGSQVGKDSGQFPHPLAHYMKELTFRASNIKDHHSDSTAPSHNLSTAFRLIKEMQKRFKTEEAEEREKEGAVKQDKLILSQNKLNPKLKDLLIRPNIIQKRITGSLEAHTNGFRYTSLRGDRIDVLYNNIKHAFFQPCDNEMIILLHFHLKNPVLWGKKKYKDVQFYTEVGEITTDLGKYHHMQDRDDMQSEQQEREMRRRLNAAFNSFCEKVSRLTNDQFEFDSPFAGLGFFGVPYRSATTLKPTASCLVNLTEWPTFIVTLSEVELVHFERVSLQLKNFDMVFIFKDYKIKPQMVAQIPMSSIDKIKEWLHTCDIWYSEGIQSLNWAKVMKTITDDLEAFFEEGGWSFLNVESDNEEAMDDSDDSDAYDPEEEDASAGSGSESDEDESEGEETESDDDDEGSLDSDESEGKDWSDLEEEAANADKRREVEEPSRDRDRKRPHSSKSGPSHKRRK

Target ORF information:

RefSeq Version	NM_060420.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans FACT complex subunit spt-16 (spt-16), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060420.4

ORF Insert Sequence:

ATGTCTGGAA AACGGGCAGT TCTCAACAAG GATCTCTTTT TCCAGAGAGC GGAGAGGCTT 
TATGAGCACT GGGAGAAGGG AGCCGATGGT CTAGATTCGA TCAAATCACT TGCTTTTGTA 
TACGGAGAGA CTGATAATCC ATATACAAAG ACCAGCGCAC TATTTACCTG GCTGTTTGGC 
CACGAAATCG CTGACACAGT CCTGTTGCTT CTCAAAGATC ACATTTACAT TCTCGGAAGT 
AATCGCAAAG TCGAATTCTT CGGATCAGTC ACTGGAGACA ATCAGAGCTC AGGAAAAGTG 
CCAACAGTCA GCACACTGCT CCGCGATAAA ACCGACAAAG ACGCTGGAAA CTTTGAAAAG 
CTCATCGATC ACATCAAAAG TGCCGGCGGA GATGTTGGAA ATTTCGTCAA AGAGAAATTC 
AGCTCGGAAT TCGTCAGTTC GTGGAATAAG GCGCTCGAGG AAGGCGGTGT CAATAAGAAT 
GACGTCACTC TCGCGTTTAC TCATCTGTTC GCAGTGAAAG ACGATAAGGA GATGGATTTG 
ATTCGAAAAT CAGCACAAGC TACAACCGCA TCATGGACAG CTGCAAGAGC TCGCTACGTA 
GAGATCATTG ATCAAGAAAA GCGAGTCCGT CACTCTGTTC TCTCCAATGA GTTTGCCGCC 
TTCATGAAGG ATTCAAAAGT ACAACAGGCA CTGGCCAAGT ATGAAGCCGA CACATGCTAT 
GATCCAATCG TAATGTCCGG TGGAAATTAC TCGTTCAAAT GGAATCATGA AAGCTCGGAA 
TCTCATCTTC ACAGCCAGTT TGGAACGATC ATCACTTCGT TCGGAGCTCG TTTATCAGAA 
TATTGCACAA ATTTGACGCG AACTATGCTC ATCTTCCCAT CTTCTGAACT TGAGACTGCC 
TATGAAGCGA TTCTTGCCGC CGAGCTGGCA GTAATTGCTG CTCTGAAGCC AGGAGCCAAG 
CTGAGCGATG TTTATAAAAT TGGAATCGAT ACATTGACGG AGAAAAGTCC AAAACTTGCT 
GAAACTCTGA ACAAGAAAGA GCTTGGATTT GCGACTGGAA TCGAATTCCG TGAGAGTCGT 
CTTGCTATCA GTGCGAAGTG TGACGAGGTT GTCAAAGCAG GAATGGTCTT CATCGTTTAT 
ATTGGTGTCG ATAGTATTCC AAACAAAAAT AAAGGAGAAA AAGGAAAACC CGCCGCAATT 
GCAATCTCGG ATACAATTTT GGTTAAAGAA GAAGGCGATA ACGAGATTCT CACCGAAAAA 
GCCAAATCAC GCCTGAAGTC CAACGTCATC AAATTCAAAG AGGAGCAGGA AAACCGTGAA 
GCTGAAAAGG ACAATGATCA GAAGAAAATG CTCGGAAGAG GTCAGAGAAG TGTCGTTCTA 
ACTGATCAGA CGAGAAATAA GACGACAAAC GAAGAGTTAC GTAAGGAACG TCAGAAAGAG 
CTCGGAGTTC AGTTGAATGA GCTCGCAAAA GCTCGTCTTT CAAAGCAAGG CGGTGGGACC 
GATGAGAAAA AGTCGAAGAA ATCGAATGTG TCATATAAAA CCGAAGAGAG ATTCCCACAA 
GATGCAGACG TTCAGAAGAT GTTGATTTTC GTGGATCGCA AGTATGATTC TGTGGTTGTT 
CCAATATTTG GAATTCCTGT CCCATTCCAT ATCTCCATGA TAAAGAATTG TTCGCAATCA 
GTTGAAGGAG ATTTCACATA TCTTCGAATC AACTTTGCCA CTCCAGGATC TCAAGTTGGA 
AAGGATAGTG GGCAATTCCC ACATCCACTT GCACACTATA TGAAGGAGCT CACGTTCCGT 
GCGAGTAACA TCAAGGATCA TCACAGTGAT TCGACTGCGC CGAGCCATAA TTTGAGCACC 
GCATTCCGAC TCATCAAGGA AATGCAGAAG AGATTCAAGA CTGAAGAGGC TGAAGAGCGG 
GAGAAGGAAG GAGCTGTGAA GCAGGACAAA TTGATTTTGA GCCAGAACAA ATTGAATCCG 
AAGCTCAAGG ATCTTCTGAT TCGTCCGAAT ATTATTCAAA AGCGTATCAC TGGATCGTTG 
GAGGCCCACA CTAATGGATT TAGATACACA TCTCTTCGTG GTGATCGTAT TGATGTTCTC 
TACAACAACA TCAAACATGC ATTCTTCCAA CCGTGCGACA ATGAAATGAT CATCCTTCTG 
CACTTCCATC TCAAGAATCC AGTATTGTGG GGAAAGAAGA AGTATAAGGA TGTTCAATTC 
TACACAGAAG TCGGAGAAAT CACTACGGAT CTTGGAAAGT ATCATCATAT GCAAGATCGT 
GATGATATGC AGAGTGAACA GCAAGAACGT GAAATGCGTC GTCGTCTCAA TGCTGCATTT 
AATAGTTTCT GCGAGAAGGT GTCTCGTCTC ACAAATGATC AATTTGAATT CGATTCTCCG 
TTCGCTGGAC TCGGATTCTT TGGAGTTCCG TATCGTTCGG CTACCACACT CAAACCAACT 
GCATCGTGCC TTGTTAATCT CACCGAATGG CCTACATTCA TCGTCACACT CTCCGAAGTT 
GAACTTGTCC ACTTTGAACG TGTCTCTCTT CAACTCAAGA ATTTCGATAT GGTCTTCATC 
TTCAAAGATT ACAAGATCAA ACCTCAGATG GTCGCCCAAA TCCCGATGAG CAGCATCGAT 
AAGATAAAAG AATGGCTTCA TACTTGTGAT ATTTGGTACA GTGAAGGAAT TCAATCACTG 
AATTGGGCAA AAGTTATGAA GACAATCACT GATGATCTTG AAGCATTCTT TGAAGAAGGT 
GGATGGAGTT TCCTTAATGT TGAATCTGAT AATGAAGAGG CAATGGATGA TTCTGATGAT 
TCGGATGCTT ATGATCCTGA AGAAGAAGAT GCATCTGCTG GATCAGGAAG TGAAAGTGAT 
GAAGATGAAA GTGAAGGTGA AGAGACTGAA TCCGATGATG ATGATGAAGG ATCGTTGGAT 
TCCGATGAAT CAGAAGGAAA AGATTGGTCA GATTTGGAGG AAGAAGCAGC GAATGCTGAT 
AAGAGACGTG AAGTCGAAGA ACCTTCACGT GATCGTGATC GTAAGAGACC ACATTCGAGC 
AAGTCTGGAC CATCGCACAA ACGCAGAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spt-16 ( NM_060420.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492821.1 Caenorhabditis elegans FACT complex subunit spt-16 (spt-16), mRNA.

Price & Availability↑

CloneID	OCa336658
Clone ID Related Accession (Same CDS sequence)	NM_060420.4 , NM_060420.5
Accession Version	NM_060420.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Caenorhabditis elegans
Product	FACT complex subunit spt-16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_060420.4.

ATGTCTGGAA AACGGGCAGT TCTCAACAAG GATCTCTTTT TCCAGAGAGC GGAGAGGCTT 
TATGAGCACT GGGAGAAGGG AGCCGATGGT CTAGATTCGA TCAAATCACT TGCTTTTGTA 
TACGGAGAGA CTGATAATCC ATATACAAAG ACCAGCGCAC TATTTACCTG GCTGTTTGGC 
CACGAAATCG CTGACACAGT CCTGTTGCTT CTCAAAGATC ACATTTACAT TCTCGGAAGT 
AATCGCAAAG TCGAATTCTT CGGATCAGTC ACTGGAGACA ATCAGAGCTC AGGAAAAGTG 
CCAACAGTCA GCACACTGCT CCGCGATAAA ACCGACAAAG ACGCTGGAAA CTTTGAAAAG 
CTCATCGATC ACATCAAAAG TGCCGGCGGA GATGTTGGAA ATTTCGTCAA AGAGAAATTC 
AGCTCGGAAT TCGTCAGTTC GTGGAATAAG GCGCTCGAGG AAGGCGGTGT CAATAAGAAT 
GACGTCACTC TCGCGTTTAC TCATCTGTTC GCAGTGAAAG ACGATAAGGA GATGGATTTG 
ATTCGAAAAT CAGCACAAGC TACAACCGCA TCATGGACAG CTGCAAGAGC TCGCTACGTA 
GAGATCATTG ATCAAGAAAA GCGAGTCCGT CACTCTGTTC TCTCCAATGA GTTTGCCGCC 
TTCATGAAGG ATTCAAAAGT ACAACAGGCA CTGGCCAAGT ATGAAGCCGA CACATGCTAT 
GATCCAATCG TAATGTCCGG TGGAAATTAC TCGTTCAAAT GGAATCATGA AAGCTCGGAA 
TCTCATCTTC ACAGCCAGTT TGGAACGATC ATCACTTCGT TCGGAGCTCG TTTATCAGAA 
TATTGCACAA ATTTGACGCG AACTATGCTC ATCTTCCCAT CTTCTGAACT TGAGACTGCC 
TATGAAGCGA TTCTTGCCGC CGAGCTGGCA GTAATTGCTG CTCTGAAGCC AGGAGCCAAG 
CTGAGCGATG TTTATAAAAT TGGAATCGAT ACATTGACGG AGAAAAGTCC AAAACTTGCT 
GAAACTCTGA ACAAGAAAGA GCTTGGATTT GCGACTGGAA TCGAATTCCG TGAGAGTCGT 
CTTGCTATCA GTGCGAAGTG TGACGAGGTT GTCAAAGCAG GAATGGTCTT CATCGTTTAT 
ATTGGTGTCG ATAGTATTCC AAACAAAAAT AAAGGAGAAA AAGGAAAACC CGCCGCAATT 
GCAATCTCGG ATACAATTTT GGTTAAAGAA GAAGGCGATA ACGAGATTCT CACCGAAAAA 
GCCAAATCAC GCCTGAAGTC CAACGTCATC AAATTCAAAG AGGAGCAGGA AAACCGTGAA 
GCTGAAAAGG ACAATGATCA GAAGAAAATG CTCGGAAGAG GTCAGAGAAG TGTCGTTCTA 
ACTGATCAGA CGAGAAATAA GACGACAAAC GAAGAGTTAC GTAAGGAACG TCAGAAAGAG 
CTCGGAGTTC AGTTGAATGA GCTCGCAAAA GCTCGTCTTT CAAAGCAAGG CGGTGGGACC 
GATGAGAAAA AGTCGAAGAA ATCGAATGTG TCATATAAAA CCGAAGAGAG ATTCCCACAA 
GATGCAGACG TTCAGAAGAT GTTGATTTTC GTGGATCGCA AGTATGATTC TGTGGTTGTT 
CCAATATTTG GAATTCCTGT CCCATTCCAT ATCTCCATGA TAAAGAATTG TTCGCAATCA 
GTTGAAGGAG ATTTCACATA TCTTCGAATC AACTTTGCCA CTCCAGGATC TCAAGTTGGA 
AAGGATAGTG GGCAATTCCC ACATCCACTT GCACACTATA TGAAGGAGCT CACGTTCCGT 
GCGAGTAACA TCAAGGATCA TCACAGTGAT TCGACTGCGC CGAGCCATAA TTTGAGCACC 
GCATTCCGAC TCATCAAGGA AATGCAGAAG AGATTCAAGA CTGAAGAGGC TGAAGAGCGG 
GAGAAGGAAG GAGCTGTGAA GCAGGACAAA TTGATTTTGA GCCAGAACAA ATTGAATCCG 
AAGCTCAAGG ATCTTCTGAT TCGTCCGAAT ATTATTCAAA AGCGTATCAC TGGATCGTTG 
GAGGCCCACA CTAATGGATT TAGATACACA TCTCTTCGTG GTGATCGTAT TGATGTTCTC 
TACAACAACA TCAAACATGC ATTCTTCCAA CCGTGCGACA ATGAAATGAT CATCCTTCTG 
CACTTCCATC TCAAGAATCC AGTATTGTGG GGAAAGAAGA AGTATAAGGA TGTTCAATTC 
TACACAGAAG TCGGAGAAAT CACTACGGAT CTTGGAAAGT ATCATCATAT GCAAGATCGT 
GATGATATGC AGAGTGAACA GCAAGAACGT GAAATGCGTC GTCGTCTCAA TGCTGCATTT 
AATAGTTTCT GCGAGAAGGT GTCTCGTCTC ACAAATGATC AATTTGAATT CGATTCTCCG 
TTCGCTGGAC TCGGATTCTT TGGAGTTCCG TATCGTTCGG CTACCACACT CAAACCAACT 
GCATCGTGCC TTGTTAATCT CACCGAATGG CCTACATTCA TCGTCACACT CTCCGAAGTT 
GAACTTGTCC ACTTTGAACG TGTCTCTCTT CAACTCAAGA ATTTCGATAT GGTCTTCATC 
TTCAAAGATT ACAAGATCAA ACCTCAGATG GTCGCCCAAA TCCCGATGAG CAGCATCGAT 
AAGATAAAAG AATGGCTTCA TACTTGTGAT ATTTGGTACA GTGAAGGAAT TCAATCACTG 
AATTGGGCAA AAGTTATGAA GACAATCACT GATGATCTTG AAGCATTCTT TGAAGAAGGT 
GGATGGAGTT TCCTTAATGT TGAATCTGAT AATGAAGAGG CAATGGATGA TTCTGATGAT 
TCGGATGCTT ATGATCCTGA AGAAGAAGAT GCATCTGCTG GATCAGGAAG TGAAAGTGAT 
GAAGATGAAA GTGAAGGTGA AGAGACTGAA TCCGATGATG ATGATGAAGG ATCGTTGGAT 
TCCGATGAAT CAGAAGGAAA AGATTGGTCA GATTTGGAGG AAGAAGCAGC GAATGCTGAT 
AAGAGACGTG AAGTCGAAGA ACCTTCACGT GATCGTGATC GTAAGAGACC ACATTCGAGC 
AAGTCTGGAC CATCGCACAA ACGCAGAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492821.1
CDS	8..3100
Translation	MSGKRAVLNKDLFFQRAERLYEHWEKGADGLDSIKSLAFVYGETDNPYTKTSALFTWLFGHEIADTVLLLLKDHIYILGSNRKVEFFGSVTGDNQSSGKVPTVSTLLRDKTDKDAGNFEKLIDHIKSAGGDVGNFVKEKFSSEFVSSWNKALEEGGVNKNDVTLAFTHLFAVKDDKEMDLIRKSAQATTASWTAARARYVEIIDQEKRVRHSVLSNEFAAFMKDSKVQQALAKYEADTCYDPIVMSGGNYSFKWNHESSESHLHSQFGTIITSFGARLSEYCTNLTRTMLIFPSSELETAYEAILAAELAVIAALKPGAKLSDVYKIGIDTLTEKSPKLAETLNKKELGFATGIEFRESRLAISAKCDEVVKAGMVFIVYIGVDSIPNKNKGEKGKPAAIAISDTILVKEEGDNEILTEKAKSRLKSNVIKFKEEQENREAEKDNDQKKMLGRGQRSVVLTDQTRNKTTNEELRKERQKELGVQLNELAKARLSKQGGGTDEKKSKKSNVSYKTEERFPQDADVQKMLIFVDRKYDSVVVPIFGIPVPFHISMIKNCSQSVEGDFTYLRINFATPGSQVGKDSGQFPHPLAHYMKELTFRASNIKDHHSDSTAPSHNLSTAFRLIKEMQKRFKTEEAEEREKEGAVKQDKLILSQNKLNPKLKDLLIRPNIIQKRITGSLEAHTNGFRYTSLRGDRIDVLYNNIKHAFFQPCDNEMIILLHFHLKNPVLWGKKKYKDVQFYTEVGEITTDLGKYHHMQDRDDMQSEQQEREMRRRLNAAFNSFCEKVSRLTNDQFEFDSPFAGLGFFGVPYRSATTLKPTASCLVNLTEWPTFIVTLSEVELVHFERVSLQLKNFDMVFIFKDYKIKPQMVAQIPMSSIDKIKEWLHTCDIWYSEGIQSLNWAKVMKTITDDLEAFFEEGGWSFLNVESDNEEAMDDSDDSDAYDPEEEDASAGSGSESDEDESEGEETESDDDDEGSLDSDESEGKDWSDLEEEAANADKRREVEEPSRDRDRKRPHSSKSGPSHKRRK

Target ORF information:

RefSeq Version	NM_060420.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans FACT complex subunit spt-16 (spt-16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060420.5

ORF Insert Sequence:

ATGTCTGGAA AACGGGCAGT TCTCAACAAG GATCTCTTTT TCCAGAGAGC GGAGAGGCTT 
TATGAGCACT GGGAGAAGGG AGCCGATGGT CTAGATTCGA TCAAATCACT TGCTTTTGTA 
TACGGAGAGA CTGATAATCC ATATACAAAG ACCAGCGCAC TATTTACCTG GCTGTTTGGC 
CACGAAATCG CTGACACAGT CCTGTTGCTT CTCAAAGATC ACATTTACAT TCTCGGAAGT 
AATCGCAAAG TCGAATTCTT CGGATCAGTC ACTGGAGACA ATCAGAGCTC AGGAAAAGTG 
CCAACAGTCA GCACACTGCT CCGCGATAAA ACCGACAAAG ACGCTGGAAA CTTTGAAAAG 
CTCATCGATC ACATCAAAAG TGCCGGCGGA GATGTTGGAA ATTTCGTCAA AGAGAAATTC 
AGCTCGGAAT TCGTCAGTTC GTGGAATAAG GCGCTCGAGG AAGGCGGTGT CAATAAGAAT 
GACGTCACTC TCGCGTTTAC TCATCTGTTC GCAGTGAAAG ACGATAAGGA GATGGATTTG 
ATTCGAAAAT CAGCACAAGC TACAACCGCA TCATGGACAG CTGCAAGAGC TCGCTACGTA 
GAGATCATTG ATCAAGAAAA GCGAGTCCGT CACTCTGTTC TCTCCAATGA GTTTGCCGCC 
TTCATGAAGG ATTCAAAAGT ACAACAGGCA CTGGCCAAGT ATGAAGCCGA CACATGCTAT 
GATCCAATCG TAATGTCCGG TGGAAATTAC TCGTTCAAAT GGAATCATGA AAGCTCGGAA 
TCTCATCTTC ACAGCCAGTT TGGAACGATC ATCACTTCGT TCGGAGCTCG TTTATCAGAA 
TATTGCACAA ATTTGACGCG AACTATGCTC ATCTTCCCAT CTTCTGAACT TGAGACTGCC 
TATGAAGCGA TTCTTGCCGC CGAGCTGGCA GTAATTGCTG CTCTGAAGCC AGGAGCCAAG 
CTGAGCGATG TTTATAAAAT TGGAATCGAT ACATTGACGG AGAAAAGTCC AAAACTTGCT 
GAAACTCTGA ACAAGAAAGA GCTTGGATTT GCGACTGGAA TCGAATTCCG TGAGAGTCGT 
CTTGCTATCA GTGCGAAGTG TGACGAGGTT GTCAAAGCAG GAATGGTCTT CATCGTTTAT 
ATTGGTGTCG ATAGTATTCC AAACAAAAAT AAAGGAGAAA AAGGAAAACC CGCCGCAATT 
GCAATCTCGG ATACAATTTT GGTTAAAGAA GAAGGCGATA ACGAGATTCT CACCGAAAAA 
GCCAAATCAC GCCTGAAGTC CAACGTCATC AAATTCAAAG AGGAGCAGGA AAACCGTGAA 
GCTGAAAAGG ACAATGATCA GAAGAAAATG CTCGGAAGAG GTCAGAGAAG TGTCGTTCTA 
ACTGATCAGA CGAGAAATAA GACGACAAAC GAAGAGTTAC GTAAGGAACG TCAGAAAGAG 
CTCGGAGTTC AGTTGAATGA GCTCGCAAAA GCTCGTCTTT CAAAGCAAGG CGGTGGGACC 
GATGAGAAAA AGTCGAAGAA ATCGAATGTG TCATATAAAA CCGAAGAGAG ATTCCCACAA 
GATGCAGACG TTCAGAAGAT GTTGATTTTC GTGGATCGCA AGTATGATTC TGTGGTTGTT 
CCAATATTTG GAATTCCTGT CCCATTCCAT ATCTCCATGA TAAAGAATTG TTCGCAATCA 
GTTGAAGGAG ATTTCACATA TCTTCGAATC AACTTTGCCA CTCCAGGATC TCAAGTTGGA 
AAGGATAGTG GGCAATTCCC ACATCCACTT GCACACTATA TGAAGGAGCT CACGTTCCGT 
GCGAGTAACA TCAAGGATCA TCACAGTGAT TCGACTGCGC CGAGCCATAA TTTGAGCACC 
GCATTCCGAC TCATCAAGGA AATGCAGAAG AGATTCAAGA CTGAAGAGGC TGAAGAGCGG 
GAGAAGGAAG GAGCTGTGAA GCAGGACAAA TTGATTTTGA GCCAGAACAA ATTGAATCCG 
AAGCTCAAGG ATCTTCTGAT TCGTCCGAAT ATTATTCAAA AGCGTATCAC TGGATCGTTG 
GAGGCCCACA CTAATGGATT TAGATACACA TCTCTTCGTG GTGATCGTAT TGATGTTCTC 
TACAACAACA TCAAACATGC ATTCTTCCAA CCGTGCGACA ATGAAATGAT CATCCTTCTG 
CACTTCCATC TCAAGAATCC AGTATTGTGG GGAAAGAAGA AGTATAAGGA TGTTCAATTC 
TACACAGAAG TCGGAGAAAT CACTACGGAT CTTGGAAAGT ATCATCATAT GCAAGATCGT 
GATGATATGC AGAGTGAACA GCAAGAACGT GAAATGCGTC GTCGTCTCAA TGCTGCATTT 
AATAGTTTCT GCGAGAAGGT GTCTCGTCTC ACAAATGATC AATTTGAATT CGATTCTCCG 
TTCGCTGGAC TCGGATTCTT TGGAGTTCCG TATCGTTCGG CTACCACACT CAAACCAACT 
GCATCGTGCC TTGTTAATCT CACCGAATGG CCTACATTCA TCGTCACACT CTCCGAAGTT 
GAACTTGTCC ACTTTGAACG TGTCTCTCTT CAACTCAAGA ATTTCGATAT GGTCTTCATC 
TTCAAAGATT ACAAGATCAA ACCTCAGATG GTCGCCCAAA TCCCGATGAG CAGCATCGAT 
AAGATAAAAG AATGGCTTCA TACTTGTGAT ATTTGGTACA GTGAAGGAAT TCAATCACTG 
AATTGGGCAA AAGTTATGAA GACAATCACT GATGATCTTG AAGCATTCTT TGAAGAAGGT 
GGATGGAGTT TCCTTAATGT TGAATCTGAT AATGAAGAGG CAATGGATGA TTCTGATGAT 
TCGGATGCTT ATGATCCTGA AGAAGAAGAT GCATCTGCTG GATCAGGAAG TGAAAGTGAT 
GAAGATGAAA GTGAAGGTGA AGAGACTGAA TCCGATGATG ATGATGAAGG ATCGTTGGAT 
TCCGATGAAT CAGAAGGAAA AGATTGGTCA GATTTGGAGG AAGAAGCAGC GAATGCTGAT 
AAGAGACGTG AAGTCGAAGA ACCTTCACGT GATCGTGATC GTAAGAGACC ACATTCGAGC 
AAGTCTGGAC CATCGCACAA ACGCAGAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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spt-16 cDNA ORF clone, Caenorhabditis elegans

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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