hum-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	hum-1
Entrez Gene ID	172698
Full Name	Heavy chain, Unconventional Myosin
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_059992.3	NP_492393.1	Heavy chain, Unconventional Myosin
NM_059992.4	NP_492393.1	Heavy chain, Unconventional Myosin

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYO1E	NP_004989.2
Pan troglodytes (chimpanzee)	MYO1E	XP_510448.2
Bos taurus (cattle)	MYO1E	XP_002690899.2
Mus musculus (house mouse)	Myo1e	NP_851417.2
Gallus gallus (chicken)	MYO1E	XP_413782.4
Caenorhabditis elegans (roundworm)	hum-1	NP_492393.1
Macaca mulatta (Rhesus monkey)	MYO1E	XP_002804857.1
Canis lupus familiaris (dog)	MYO1E	XP_535500.3
Rattus norvegicus (Norway rat)	Myo1e	NP_775124.1
Danio rerio (zebrafish)	myo1ea	XP_005168984.1
Xenopus tropicalis (tropical clawed frog)	myo1e.2	NP_001011082.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hum-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hum-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335645	NM_059992.4 Latest version!	Caenorhabditis elegans Heavy chain, Unconventional Myosin (hum-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa335645	NM_059992.3	Caenorhabditis elegans Heavy chain, Unconventional Myosin (hum-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hum-1 ( NM_059992.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492393.1 Caenorhabditis elegans Heavy chain, Unconventional Myosin (hum-1), partial mRNA.

Price & Availability↑

CloneID	OCa335645
Clone ID Related Accession (Same CDS sequence)	NM_059992.3 , NM_059992.4
Accession Version	NM_059992.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554652800000
Organism	Caenorhabditis elegans
Product	Heavy chain, Unconventional Myosin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059992.2. COMPLETENESS: incomplete on both ends.

ATGGCGTTCC ACTGGCAATC AAAAGTCAAT GTTCAGCATG TCGGGGTCGA TGACATGGTC 
CTTCTGCCAA AACTCACAGA GCAAAGTATC GTTGAAAATT TAAAGAAGAG GCTTCAAGCT 
AACAGTATTT TTACATATAT TGGTCCTGTA CTGATATCGG TAAATCCCTT CAAACAAATG 
CCATATTTTA CGGAGAAAGA AATGCTTCTA TATCAGGGAG CTGCTCAATA TGAAAATGCG 
CCACATATCT ATGCATTGGC TGATAACATG TACAGAAATA TGCTCATTGA TAACGAGTCC 
CAATGTGTTA TTATATCTGG AGAATCCGGT GCCGGTAAAA CAGTCAACGC GAAGTTCATT 
ATGAATTATA TTTCTCGTAT TTCGGGAGGA GGGCAGAAAG TTCAGCACAT CAAAGATGTT 
ATTTTGCAAT CAAATCCACT TCTCGAAGCT TTCGGAAACA GTGCAACAGT CCGTAACTGG 
AACAGTTCGA GATTTGGAAA ATACGTAGAA ATTGTGTTCA GTCGAGGTGG AGAACCTATT 
GGTGGAAAAC TTTCCAACTT TCTTTTGGAA AAGTCTCGAG TTGTTCATCA AAATGAAGGA 
GATCGAAACT TCCATGTTTT TTATCAATTA TGCGCTGGCG CAGACAAGAA CTTGAGAAGT 
ACTTTCGGAA TTGGAGAACT TCAATATTAC AACTATTTAA ATATGAGTGG AGTTTTCAAA 
GCAGACGATA CGGACGATGG GAAGGAATTT GAGAGCACCC TGCACGCAAT GAAAGTTGTC 
GGTGTTAATG ATCAAGATCA ATTAGAAGTT TTGCGAATTG TGGCAACTGT TCTCCATATC 
GGAAATATCA CTTTCACCGA GGAGAATAAT TTTGCTGCAG TTAGTGGAAA AGATTATCTT 
GAATATCCTG CCTTCCTTCT GGGACTCACT AGTGCAGACA TTGAGGCAAA GCTTACTGGT 
CGTAAAATGG AGAGCAAATG GGGTACACAA AAGGAAGAAA TCGATATGAA GCTTAACGTT 
GAGCAGGCGA GCTATACCAG AGACGCATGG GTTAAAGCAA TTTACGCAAG ATTATTCGAT 
TATCTTGTAA AAAAAGTGAA TGACGCAATG AATATCACTT CACAATCGAC TTCAGACAAC 
TTTTCTGTTG GAATTCTTGA TATCTACGGT TTCGAAATTT TCAACAATAA TGGATTTGAA 
CAATTTTGTA TCAACTTTGT CAATGAAAAA CTTCAACAAA TCTTCATCGA ATTAACGCTA 
AAAGCAGAAC AGGAAGAATA TGTTCGTGAA GGAATCAAAT GGACAGAAAT CGATTATTTC 
GATAACAAAA TCGTGTGTGA TCTGATTGAA ACGAAGCGTC CACCTGGAAT AATGAGCCTT 
CTTGATGATA CATGTGCTCA AAATCATGGA CAACGAGAAG GTGTCGATCG TCAATTGCTC 
ACGACTCTTT CGAAATCATT TGCTGGACAT CCCCATTTCG GCCCTGGCTC AGATTCATTC 
GTTATCAAAC ATTATGCTGG AGATGTTACA TATAATGTAG ATGGATTCTG TGATAGAAAC 
AGAGATGTTC TTTATCCAGA TCTTATTCTA CTCATGCAAA AGTCTTCACG CCCATTCATT 
CAAGCTTTAT TCCCTGAAAA TGTAGCTGCG TCAGCTGGAA AACGACCAAC AACATTCTCA 
ACTAAGATTC GAACTCAAGC TAATACTCTT GTGGAATCCC TGATGAAATG CTCACCTCAT 
TACGTACGAT GTATCAAGCC AAATGAAACA AAACGACCAA ATGATTGGGA AGAGAGTCGA 
GTGAAGCATC AGGTTGAATA TTTGGGTCTT CGTGAGAATA TTCGAGTGAG AAGAGCTGGT 
TTTGCATATC GAAGAGCTTT CGACAAATTT GCCCAACGAT ATGCCATCGT CTCTCCTCAA 
ACGTGGCCAT GTTTCCAAGG AGATCAGCAA AGAGCTTGTG AGATAATCTG CGATTCAGTT 
CATATGGAAA AGAATCAGTA TCAGATGGGA AAAACTAAGA TATTTGTAAA GAATCCCGAA 
TCGCTGTTTT TATTGGAAGA GACTCGTGAA CGCAAGTTTG ATGGTTACGC AAGAGTCATT 
CAAAAAGCCT GGAGACAATT CTCGGCTAGA AAGCAGCACA TTAAGCAAAA GGAGCAAGCT 
GCCGACCTCA TGTATGGAAA AAAGGAACGC CGTCGCTACT CATTAAACCG AAACTTTGTC 
GGAGATTACA TTGGATTAGA GCATCATCCG ACACTTCAGT CCCTGGTTGG CAAGCGTCAG 
AGAGTGCTGT TTGCATGCAC TGCTAACAAA TATGATAGAA AGTTTAGGGT GACAAAACTT 
GATTTACTTT TGACTGTCAA TCATTTGACT CTTATTGGAA AAGAAAAAGT GAAGAATGGT 
CCCGAAAAGG GGAAGATCGT TGAAGTTATC AAAAGACAAT TTGATCTACC TCAAATCAAA 
TCAATTGGTC TATCTCCATA TCAAGACGAT TTTGTGATAT TATATCTGGG AAACGATGAT 
TATTCTTCTC TTCTCGAAAC ACCATTCAAA ACTGAATTCT GTACAGCTCT CTCAAAAGCA 
TATAAAGAAC GAACAAACGG TACTCTTCAT CTCGATTTCC GTAGTTCTCA TGTTGTTAGT 
TACAAAAAGA TGAAATTCGA TTTCTCCGAT GGAAAACGAA CGGTTCAGTT TGGAAACGAT 
GGAACTTCGT CTGCAGAGAA GACATTGAAA CCAAATGGAA AAGTTTTGAA TGTTAGCATT 
GGTACTGGAT TACCGAATAC TACAAGGCCA TCAACTGAAC GTCCACAAGG TGGGTACACC 
CCGCGAAGAG ATCAATTGAG AACATCAACA CGACGAACGA AACAGAATAA TCAAAGTTAT 
GGACAAAATG GCCAATCTCA GGCAATGAGA GCTCCGGTTC CCGCTCATGG AATGAATAAC 
AATTACAATC AGACACCAGC TCCAGTATCG ACTAATCATC AATATAGCCA AGAACCTGCG 
CGAATTCCAG TGATGGGAAA CGTCATAAAT CAGTTGAATA ACATGAATCT ATCTGGAAAC 
GGAAACTCTC CAGCTGGACG AGGACCTCCG CCAGCGCGTG GACCAAAACC TCCTCCACCA 
GCAAAGCCAA AGCTTAATCC TGTCGTTATT GCTGTCTATC CATATGAAGC TCAAGATGTT 
GATGAGCTCA GCTTTGAAGC TGGAGCTGAA ATTGAACTCA TGAACAAAGA TGCTTCTGGA 
TGGTGGCAAG GAAAAGTGAA CAATCGAGTT GGTCTTTTCC CCGGAAATTA TGTCAAAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492393.1
CDS	1..3303
Translation	MAFHWQSKVNVQHVGVDDMVLLPKLTEQSIVENLKKRLQANSIFTYIGPVLISVNPFKQMPYFTEKEMLLYQGAAQYENAPHIYALADNMYRNMLIDNESQCVIISGESGAGKTVNAKFIMNYISRISGGGQKVQHIKDVILQSNPLLEAFGNSATVRNWNSSRFGKYVEIVFSRGGEPIGGKLSNFLLEKSRVVHQNEGDRNFHVFYQLCAGADKNLRSTFGIGELQYYNYLNMSGVFKADDTDDGKEFESTLHAMKVVGVNDQDQLEVLRIVATVLHIGNITFTEENNFAAVSGKDYLEYPAFLLGLTSADIEAKLTGRKMESKWGTQKEEIDMKLNVEQASYTRDAWVKAIYARLFDYLVKKVNDAMNITSQSTSDNFSVGILDIYGFEIFNNNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVREGIKWTEIDYFDNKIVCDLIETKRPPGIMSLLDDTCAQNHGQREGVDRQLLTTLSKSFAGHPHFGPGSDSFVIKHYAGDVTYNVDGFCDRNRDVLYPDLILLMQKSSRPFIQALFPENVAASAGKRPTTFSTKIRTQANTLVESLMKCSPHYVRCIKPNETKRPNDWEESRVKHQVEYLGLRENIRVRRAGFAYRRAFDKFAQRYAIVSPQTWPCFQGDQQRACEIICDSVHMEKNQYQMGKTKIFVKNPESLFLLEETRERKFDGYARVIQKAWRQFSARKQHIKQKEQAADLMYGKKERRRYSLNRNFVGDYIGLEHHPTLQSLVGKRQRVLFACTANKYDRKFRVTKLDLLLTVNHLTLIGKEKVKNGPEKGKIVEVIKRQFDLPQIKSIGLSPYQDDFVILYLGNDDYSSLLETPFKTEFCTALSKAYKERTNGTLHLDFRSSHVVSYKKMKFDFSDGKRTVQFGNDGTSSAEKTLKPNGKVLNVSIGTGLPNTTRPSTERPQGGYTPRRDQLRTSTRRTKQNNQSYGQNGQSQAMRAPVPAHGMNNNYNQTPAPVSTNHQYSQEPARIPVMGNVINQLNNMNLSGNGNSPAGRGPPPARGPKPPPPAKPKLNPVVIAVYPYEAQDVDELSFEAGAEIELMNKDASGWWQGKVNNRVGLFPGNYVKE

Target ORF information:

RefSeq Version	NM_059992.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Heavy chain, Unconventional Myosin (hum-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059992.3

ORF Insert Sequence:

ATGGCGTTCC ACTGGCAATC AAAAGTCAAT GTTCAGCATG TCGGGGTCGA TGACATGGTC 
CTTCTGCCAA AACTCACAGA GCAAAGTATC GTTGAAAATT TAAAGAAGAG GCTTCAAGCT 
AACAGTATTT TTACATATAT TGGTCCTGTA CTGATATCGG TAAATCCCTT CAAACAAATG 
CCATATTTTA CGGAGAAAGA AATGCTTCTA TATCAGGGAG CTGCTCAATA TGAAAATGCG 
CCACATATCT ATGCATTGGC TGATAACATG TACAGAAATA TGCTCATTGA TAACGAGTCC 
CAATGTGTTA TTATATCTGG AGAATCCGGT GCCGGTAAAA CAGTCAACGC GAAGTTCATT 
ATGAATTATA TTTCTCGTAT TTCGGGAGGA GGGCAGAAAG TTCAGCACAT CAAAGATGTT 
ATTTTGCAAT CAAATCCACT TCTCGAAGCT TTCGGAAACA GTGCAACAGT CCGTAACTGG 
AACAGTTCGA GATTTGGAAA ATACGTAGAA ATTGTGTTCA GTCGAGGTGG AGAACCTATT 
GGTGGAAAAC TTTCCAACTT TCTTTTGGAA AAGTCTCGAG TTGTTCATCA AAATGAAGGA 
GATCGAAACT TCCATGTTTT TTATCAATTA TGCGCTGGCG CAGACAAGAA CTTGAGAAGT 
ACTTTCGGAA TTGGAGAACT TCAATATTAC AACTATTTAA ATATGAGTGG AGTTTTCAAA 
GCAGACGATA CGGACGATGG GAAGGAATTT GAGAGCACCC TGCACGCAAT GAAAGTTGTC 
GGTGTTAATG ATCAAGATCA ATTAGAAGTT TTGCGAATTG TGGCAACTGT TCTCCATATC 
GGAAATATCA CTTTCACCGA GGAGAATAAT TTTGCTGCAG TTAGTGGAAA AGATTATCTT 
GAATATCCTG CCTTCCTTCT GGGACTCACT AGTGCAGACA TTGAGGCAAA GCTTACTGGT 
CGTAAAATGG AGAGCAAATG GGGTACACAA AAGGAAGAAA TCGATATGAA GCTTAACGTT 
GAGCAGGCGA GCTATACCAG AGACGCATGG GTTAAAGCAA TTTACGCAAG ATTATTCGAT 
TATCTTGTAA AAAAAGTGAA TGACGCAATG AATATCACTT CACAATCGAC TTCAGACAAC 
TTTTCTGTTG GAATTCTTGA TATCTACGGT TTCGAAATTT TCAACAATAA TGGATTTGAA 
CAATTTTGTA TCAACTTTGT CAATGAAAAA CTTCAACAAA TCTTCATCGA ATTAACGCTA 
AAAGCAGAAC AGGAAGAATA TGTTCGTGAA GGAATCAAAT GGACAGAAAT CGATTATTTC 
GATAACAAAA TCGTGTGTGA TCTGATTGAA ACGAAGCGTC CACCTGGAAT AATGAGCCTT 
CTTGATGATA CATGTGCTCA AAATCATGGA CAACGAGAAG GTGTCGATCG TCAATTGCTC 
ACGACTCTTT CGAAATCATT TGCTGGACAT CCCCATTTCG GCCCTGGCTC AGATTCATTC 
GTTATCAAAC ATTATGCTGG AGATGTTACA TATAATGTAG ATGGATTCTG TGATAGAAAC 
AGAGATGTTC TTTATCCAGA TCTTATTCTA CTCATGCAAA AGTCTTCACG CCCATTCATT 
CAAGCTTTAT TCCCTGAAAA TGTAGCTGCG TCAGCTGGAA AACGACCAAC AACATTCTCA 
ACTAAGATTC GAACTCAAGC TAATACTCTT GTGGAATCCC TGATGAAATG CTCACCTCAT 
TACGTACGAT GTATCAAGCC AAATGAAACA AAACGACCAA ATGATTGGGA AGAGAGTCGA 
GTGAAGCATC AGGTTGAATA TTTGGGTCTT CGTGAGAATA TTCGAGTGAG AAGAGCTGGT 
TTTGCATATC GAAGAGCTTT CGACAAATTT GCCCAACGAT ATGCCATCGT CTCTCCTCAA 
ACGTGGCCAT GTTTCCAAGG AGATCAGCAA AGAGCTTGTG AGATAATCTG CGATTCAGTT 
CATATGGAAA AGAATCAGTA TCAGATGGGA AAAACTAAGA TATTTGTAAA GAATCCCGAA 
TCGCTGTTTT TATTGGAAGA GACTCGTGAA CGCAAGTTTG ATGGTTACGC AAGAGTCATT 
CAAAAAGCCT GGAGACAATT CTCGGCTAGA AAGCAGCACA TTAAGCAAAA GGAGCAAGCT 
GCCGACCTCA TGTATGGAAA AAAGGAACGC CGTCGCTACT CATTAAACCG AAACTTTGTC 
GGAGATTACA TTGGATTAGA GCATCATCCG ACACTTCAGT CCCTGGTTGG CAAGCGTCAG 
AGAGTGCTGT TTGCATGCAC TGCTAACAAA TATGATAGAA AGTTTAGGGT GACAAAACTT 
GATTTACTTT TGACTGTCAA TCATTTGACT CTTATTGGAA AAGAAAAAGT GAAGAATGGT 
CCCGAAAAGG GGAAGATCGT TGAAGTTATC AAAAGACAAT TTGATCTACC TCAAATCAAA 
TCAATTGGTC TATCTCCATA TCAAGACGAT TTTGTGATAT TATATCTGGG AAACGATGAT 
TATTCTTCTC TTCTCGAAAC ACCATTCAAA ACTGAATTCT GTACAGCTCT CTCAAAAGCA 
TATAAAGAAC GAACAAACGG TACTCTTCAT CTCGATTTCC GTAGTTCTCA TGTTGTTAGT 
TACAAAAAGA TGAAATTCGA TTTCTCCGAT GGAAAACGAA CGGTTCAGTT TGGAAACGAT 
GGAACTTCGT CTGCAGAGAA GACATTGAAA CCAAATGGAA AAGTTTTGAA TGTTAGCATT 
GGTACTGGAT TACCGAATAC TACAAGGCCA TCAACTGAAC GTCCACAAGG TGGGTACACC 
CCGCGAAGAG ATCAATTGAG AACATCAACA CGACGAACGA AACAGAATAA TCAAAGTTAT 
GGACAAAATG GCCAATCTCA GGCAATGAGA GCTCCGGTTC CCGCTCATGG AATGAATAAC 
AATTACAATC AGACACCAGC TCCAGTATCG ACTAATCATC AATATAGCCA AGAACCTGCG 
CGAATTCCAG TGATGGGAAA CGTCATAAAT CAGTTGAATA ACATGAATCT ATCTGGAAAC 
GGAAACTCTC CAGCTGGACG AGGACCTCCG CCAGCGCGTG GACCAAAACC TCCTCCACCA 
GCAAAGCCAA AGCTTAATCC TGTCGTTATT GCTGTCTATC CATATGAAGC TCAAGATGTT 
GATGAGCTCA GCTTTGAAGC TGGAGCTGAA ATTGAACTCA TGAACAAAGA TGCTTCTGGA 
TGGTGGCAAG GAAAAGTGAA CAATCGAGTT GGTCTTTTCC CCGGAAATTA TGTCAAAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hum-1 ( NM_059992.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492393.1 Caenorhabditis elegans Heavy chain, Unconventional Myosin (hum-1), mRNA.

Price & Availability↑

CloneID	OCa335645
Clone ID Related Accession (Same CDS sequence)	NM_059992.3 , NM_059992.4
Accession Version	NM_059992.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Caenorhabditis elegans
Product	Heavy chain, Unconventional Myosin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059992.3.

ATGGCGTTCC ACTGGCAATC AAAAGTCAAT GTTCAGCATG TCGGGGTCGA TGACATGGTC 
CTTCTGCCAA AACTCACAGA GCAAAGTATC GTTGAAAATT TAAAGAAGAG GCTTCAAGCT 
AACAGTATTT TTACATATAT TGGTCCTGTA CTGATATCGG TAAATCCCTT CAAACAAATG 
CCATATTTTA CGGAGAAAGA AATGCTTCTA TATCAGGGAG CTGCTCAATA TGAAAATGCG 
CCACATATCT ATGCATTGGC TGATAACATG TACAGAAATA TGCTCATTGA TAACGAGTCC 
CAATGTGTTA TTATATCTGG AGAATCCGGT GCCGGTAAAA CAGTCAACGC GAAGTTCATT 
ATGAATTATA TTTCTCGTAT TTCGGGAGGA GGGCAGAAAG TTCAGCACAT CAAAGATGTT 
ATTTTGCAAT CAAATCCACT TCTCGAAGCT TTCGGAAACA GTGCAACAGT CCGTAACTGG 
AACAGTTCGA GATTTGGAAA ATACGTAGAA ATTGTGTTCA GTCGAGGTGG AGAACCTATT 
GGTGGAAAAC TTTCCAACTT TCTTTTGGAA AAGTCTCGAG TTGTTCATCA AAATGAAGGA 
GATCGAAACT TCCATGTTTT TTATCAATTA TGCGCTGGCG CAGACAAGAA CTTGAGAAGT 
ACTTTCGGAA TTGGAGAACT TCAATATTAC AACTATTTAA ATATGAGTGG AGTTTTCAAA 
GCAGACGATA CGGACGATGG GAAGGAATTT GAGAGCACCC TGCACGCAAT GAAAGTTGTC 
GGTGTTAATG ATCAAGATCA ATTAGAAGTT TTGCGAATTG TGGCAACTGT TCTCCATATC 
GGAAATATCA CTTTCACCGA GGAGAATAAT TTTGCTGCAG TTAGTGGAAA AGATTATCTT 
GAATATCCTG CCTTCCTTCT GGGACTCACT AGTGCAGACA TTGAGGCAAA GCTTACTGGT 
CGTAAAATGG AGAGCAAATG GGGTACACAA AAGGAAGAAA TCGATATGAA GCTTAACGTT 
GAGCAGGCGA GCTATACCAG AGACGCATGG GTTAAAGCAA TTTACGCAAG ATTATTCGAT 
TATCTTGTAA AAAAAGTGAA TGACGCAATG AATATCACTT CACAATCGAC TTCAGACAAC 
TTTTCTGTTG GAATTCTTGA TATCTACGGT TTCGAAATTT TCAACAATAA TGGATTTGAA 
CAATTTTGTA TCAACTTTGT CAATGAAAAA CTTCAACAAA TCTTCATCGA ATTAACGCTA 
AAAGCAGAAC AGGAAGAATA TGTTCGTGAA GGAATCAAAT GGACAGAAAT CGATTATTTC 
GATAACAAAA TCGTGTGTGA TCTGATTGAA ACGAAGCGTC CACCTGGAAT AATGAGCCTT 
CTTGATGATA CATGTGCTCA AAATCATGGA CAACGAGAAG GTGTCGATCG TCAATTGCTC 
ACGACTCTTT CGAAATCATT TGCTGGACAT CCCCATTTCG GCCCTGGCTC AGATTCATTC 
GTTATCAAAC ATTATGCTGG AGATGTTACA TATAATGTAG ATGGATTCTG TGATAGAAAC 
AGAGATGTTC TTTATCCAGA TCTTATTCTA CTCATGCAAA AGTCTTCACG CCCATTCATT 
CAAGCTTTAT TCCCTGAAAA TGTAGCTGCG TCAGCTGGAA AACGACCAAC AACATTCTCA 
ACTAAGATTC GAACTCAAGC TAATACTCTT GTGGAATCCC TGATGAAATG CTCACCTCAT 
TACGTACGAT GTATCAAGCC AAATGAAACA AAACGACCAA ATGATTGGGA AGAGAGTCGA 
GTGAAGCATC AGGTTGAATA TTTGGGTCTT CGTGAGAATA TTCGAGTGAG AAGAGCTGGT 
TTTGCATATC GAAGAGCTTT CGACAAATTT GCCCAACGAT ATGCCATCGT CTCTCCTCAA 
ACGTGGCCAT GTTTCCAAGG AGATCAGCAA AGAGCTTGTG AGATAATCTG CGATTCAGTT 
CATATGGAAA AGAATCAGTA TCAGATGGGA AAAACTAAGA TATTTGTAAA GAATCCCGAA 
TCGCTGTTTT TATTGGAAGA GACTCGTGAA CGCAAGTTTG ATGGTTACGC AAGAGTCATT 
CAAAAAGCCT GGAGACAATT CTCGGCTAGA AAGCAGCACA TTAAGCAAAA GGAGCAAGCT 
GCCGACCTCA TGTATGGAAA AAAGGAACGC CGTCGCTACT CATTAAACCG AAACTTTGTC 
GGAGATTACA TTGGATTAGA GCATCATCCG ACACTTCAGT CCCTGGTTGG CAAGCGTCAG 
AGAGTGCTGT TTGCATGCAC TGCTAACAAA TATGATAGAA AGTTTAGGGT GACAAAACTT 
GATTTACTTT TGACTGTCAA TCATTTGACT CTTATTGGAA AAGAAAAAGT GAAGAATGGT 
CCCGAAAAGG GGAAGATCGT TGAAGTTATC AAAAGACAAT TTGATCTACC TCAAATCAAA 
TCAATTGGTC TATCTCCATA TCAAGACGAT TTTGTGATAT TATATCTGGG AAACGATGAT 
TATTCTTCTC TTCTCGAAAC ACCATTCAAA ACTGAATTCT GTACAGCTCT CTCAAAAGCA 
TATAAAGAAC GAACAAACGG TACTCTTCAT CTCGATTTCC GTAGTTCTCA TGTTGTTAGT 
TACAAAAAGA TGAAATTCGA TTTCTCCGAT GGAAAACGAA CGGTTCAGTT TGGAAACGAT 
GGAACTTCGT CTGCAGAGAA GACATTGAAA CCAAATGGAA AAGTTTTGAA TGTTAGCATT 
GGTACTGGAT TACCGAATAC TACAAGGCCA TCAACTGAAC GTCCACAAGG TGGGTACACC 
CCGCGAAGAG ATCAATTGAG AACATCAACA CGACGAACGA AACAGAATAA TCAAAGTTAT 
GGACAAAATG GCCAATCTCA GGCAATGAGA GCTCCGGTTC CCGCTCATGG AATGAATAAC 
AATTACAATC AGACACCAGC TCCAGTATCG ACTAATCATC AATATAGCCA AGAACCTGCG 
CGAATTCCAG TGATGGGAAA CGTCATAAAT CAGTTGAATA ACATGAATCT ATCTGGAAAC 
GGAAACTCTC CAGCTGGACG AGGACCTCCG CCAGCGCGTG GACCAAAACC TCCTCCACCA 
GCAAAGCCAA AGCTTAATCC TGTCGTTATT GCTGTCTATC CATATGAAGC TCAAGATGTT 
GATGAGCTCA GCTTTGAAGC TGGAGCTGAA ATTGAACTCA TGAACAAAGA TGCTTCTGGA 
TGGTGGCAAG GAAAAGTGAA CAATCGAGTT GGTCTTTTCC CCGGAAATTA TGTCAAAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492393.1
CDS	22..3324
Translation	MAFHWQSKVNVQHVGVDDMVLLPKLTEQSIVENLKKRLQANSIFTYIGPVLISVNPFKQMPYFTEKEMLLYQGAAQYENAPHIYALADNMYRNMLIDNESQCVIISGESGAGKTVNAKFIMNYISRISGGGQKVQHIKDVILQSNPLLEAFGNSATVRNWNSSRFGKYVEIVFSRGGEPIGGKLSNFLLEKSRVVHQNEGDRNFHVFYQLCAGADKNLRSTFGIGELQYYNYLNMSGVFKADDTDDGKEFESTLHAMKVVGVNDQDQLEVLRIVATVLHIGNITFTEENNFAAVSGKDYLEYPAFLLGLTSADIEAKLTGRKMESKWGTQKEEIDMKLNVEQASYTRDAWVKAIYARLFDYLVKKVNDAMNITSQSTSDNFSVGILDIYGFEIFNNNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVREGIKWTEIDYFDNKIVCDLIETKRPPGIMSLLDDTCAQNHGQREGVDRQLLTTLSKSFAGHPHFGPGSDSFVIKHYAGDVTYNVDGFCDRNRDVLYPDLILLMQKSSRPFIQALFPENVAASAGKRPTTFSTKIRTQANTLVESLMKCSPHYVRCIKPNETKRPNDWEESRVKHQVEYLGLRENIRVRRAGFAYRRAFDKFAQRYAIVSPQTWPCFQGDQQRACEIICDSVHMEKNQYQMGKTKIFVKNPESLFLLEETRERKFDGYARVIQKAWRQFSARKQHIKQKEQAADLMYGKKERRRYSLNRNFVGDYIGLEHHPTLQSLVGKRQRVLFACTANKYDRKFRVTKLDLLLTVNHLTLIGKEKVKNGPEKGKIVEVIKRQFDLPQIKSIGLSPYQDDFVILYLGNDDYSSLLETPFKTEFCTALSKAYKERTNGTLHLDFRSSHVVSYKKMKFDFSDGKRTVQFGNDGTSSAEKTLKPNGKVLNVSIGTGLPNTTRPSTERPQGGYTPRRDQLRTSTRRTKQNNQSYGQNGQSQAMRAPVPAHGMNNNYNQTPAPVSTNHQYSQEPARIPVMGNVINQLNNMNLSGNGNSPAGRGPPPARGPKPPPPAKPKLNPVVIAVYPYEAQDVDELSFEAGAEIELMNKDASGWWQGKVNNRVGLFPGNYVKE

Target ORF information:

RefSeq Version	NM_059992.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Heavy chain, Unconventional Myosin (hum-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059992.4

ORF Insert Sequence:

ATGGCGTTCC ACTGGCAATC AAAAGTCAAT GTTCAGCATG TCGGGGTCGA TGACATGGTC 
CTTCTGCCAA AACTCACAGA GCAAAGTATC GTTGAAAATT TAAAGAAGAG GCTTCAAGCT 
AACAGTATTT TTACATATAT TGGTCCTGTA CTGATATCGG TAAATCCCTT CAAACAAATG 
CCATATTTTA CGGAGAAAGA AATGCTTCTA TATCAGGGAG CTGCTCAATA TGAAAATGCG 
CCACATATCT ATGCATTGGC TGATAACATG TACAGAAATA TGCTCATTGA TAACGAGTCC 
CAATGTGTTA TTATATCTGG AGAATCCGGT GCCGGTAAAA CAGTCAACGC GAAGTTCATT 
ATGAATTATA TTTCTCGTAT TTCGGGAGGA GGGCAGAAAG TTCAGCACAT CAAAGATGTT 
ATTTTGCAAT CAAATCCACT TCTCGAAGCT TTCGGAAACA GTGCAACAGT CCGTAACTGG 
AACAGTTCGA GATTTGGAAA ATACGTAGAA ATTGTGTTCA GTCGAGGTGG AGAACCTATT 
GGTGGAAAAC TTTCCAACTT TCTTTTGGAA AAGTCTCGAG TTGTTCATCA AAATGAAGGA 
GATCGAAACT TCCATGTTTT TTATCAATTA TGCGCTGGCG CAGACAAGAA CTTGAGAAGT 
ACTTTCGGAA TTGGAGAACT TCAATATTAC AACTATTTAA ATATGAGTGG AGTTTTCAAA 
GCAGACGATA CGGACGATGG GAAGGAATTT GAGAGCACCC TGCACGCAAT GAAAGTTGTC 
GGTGTTAATG ATCAAGATCA ATTAGAAGTT TTGCGAATTG TGGCAACTGT TCTCCATATC 
GGAAATATCA CTTTCACCGA GGAGAATAAT TTTGCTGCAG TTAGTGGAAA AGATTATCTT 
GAATATCCTG CCTTCCTTCT GGGACTCACT AGTGCAGACA TTGAGGCAAA GCTTACTGGT 
CGTAAAATGG AGAGCAAATG GGGTACACAA AAGGAAGAAA TCGATATGAA GCTTAACGTT 
GAGCAGGCGA GCTATACCAG AGACGCATGG GTTAAAGCAA TTTACGCAAG ATTATTCGAT 
TATCTTGTAA AAAAAGTGAA TGACGCAATG AATATCACTT CACAATCGAC TTCAGACAAC 
TTTTCTGTTG GAATTCTTGA TATCTACGGT TTCGAAATTT TCAACAATAA TGGATTTGAA 
CAATTTTGTA TCAACTTTGT CAATGAAAAA CTTCAACAAA TCTTCATCGA ATTAACGCTA 
AAAGCAGAAC AGGAAGAATA TGTTCGTGAA GGAATCAAAT GGACAGAAAT CGATTATTTC 
GATAACAAAA TCGTGTGTGA TCTGATTGAA ACGAAGCGTC CACCTGGAAT AATGAGCCTT 
CTTGATGATA CATGTGCTCA AAATCATGGA CAACGAGAAG GTGTCGATCG TCAATTGCTC 
ACGACTCTTT CGAAATCATT TGCTGGACAT CCCCATTTCG GCCCTGGCTC AGATTCATTC 
GTTATCAAAC ATTATGCTGG AGATGTTACA TATAATGTAG ATGGATTCTG TGATAGAAAC 
AGAGATGTTC TTTATCCAGA TCTTATTCTA CTCATGCAAA AGTCTTCACG CCCATTCATT 
CAAGCTTTAT TCCCTGAAAA TGTAGCTGCG TCAGCTGGAA AACGACCAAC AACATTCTCA 
ACTAAGATTC GAACTCAAGC TAATACTCTT GTGGAATCCC TGATGAAATG CTCACCTCAT 
TACGTACGAT GTATCAAGCC AAATGAAACA AAACGACCAA ATGATTGGGA AGAGAGTCGA 
GTGAAGCATC AGGTTGAATA TTTGGGTCTT CGTGAGAATA TTCGAGTGAG AAGAGCTGGT 
TTTGCATATC GAAGAGCTTT CGACAAATTT GCCCAACGAT ATGCCATCGT CTCTCCTCAA 
ACGTGGCCAT GTTTCCAAGG AGATCAGCAA AGAGCTTGTG AGATAATCTG CGATTCAGTT 
CATATGGAAA AGAATCAGTA TCAGATGGGA AAAACTAAGA TATTTGTAAA GAATCCCGAA 
TCGCTGTTTT TATTGGAAGA GACTCGTGAA CGCAAGTTTG ATGGTTACGC AAGAGTCATT 
CAAAAAGCCT GGAGACAATT CTCGGCTAGA AAGCAGCACA TTAAGCAAAA GGAGCAAGCT 
GCCGACCTCA TGTATGGAAA AAAGGAACGC CGTCGCTACT CATTAAACCG AAACTTTGTC 
GGAGATTACA TTGGATTAGA GCATCATCCG ACACTTCAGT CCCTGGTTGG CAAGCGTCAG 
AGAGTGCTGT TTGCATGCAC TGCTAACAAA TATGATAGAA AGTTTAGGGT GACAAAACTT 
GATTTACTTT TGACTGTCAA TCATTTGACT CTTATTGGAA AAGAAAAAGT GAAGAATGGT 
CCCGAAAAGG GGAAGATCGT TGAAGTTATC AAAAGACAAT TTGATCTACC TCAAATCAAA 
TCAATTGGTC TATCTCCATA TCAAGACGAT TTTGTGATAT TATATCTGGG AAACGATGAT 
TATTCTTCTC TTCTCGAAAC ACCATTCAAA ACTGAATTCT GTACAGCTCT CTCAAAAGCA 
TATAAAGAAC GAACAAACGG TACTCTTCAT CTCGATTTCC GTAGTTCTCA TGTTGTTAGT 
TACAAAAAGA TGAAATTCGA TTTCTCCGAT GGAAAACGAA CGGTTCAGTT TGGAAACGAT 
GGAACTTCGT CTGCAGAGAA GACATTGAAA CCAAATGGAA AAGTTTTGAA TGTTAGCATT 
GGTACTGGAT TACCGAATAC TACAAGGCCA TCAACTGAAC GTCCACAAGG TGGGTACACC 
CCGCGAAGAG ATCAATTGAG AACATCAACA CGACGAACGA AACAGAATAA TCAAAGTTAT 
GGACAAAATG GCCAATCTCA GGCAATGAGA GCTCCGGTTC CCGCTCATGG AATGAATAAC 
AATTACAATC AGACACCAGC TCCAGTATCG ACTAATCATC AATATAGCCA AGAACCTGCG 
CGAATTCCAG TGATGGGAAA CGTCATAAAT CAGTTGAATA ACATGAATCT ATCTGGAAAC 
GGAAACTCTC CAGCTGGACG AGGACCTCCG CCAGCGCGTG GACCAAAACC TCCTCCACCA 
GCAAAGCCAA AGCTTAATCC TGTCGTTATT GCTGTCTATC CATATGAAGC TCAAGATGTT 
GATGAGCTCA GCTTTGAAGC TGGAGCTGAA ATTGAACTCA TGAACAAAGA TGCTTCTGGA 
TGGTGGCAAG GAAAAGTGAA CAATCGAGTT GGTCTTTTCC CCGGAAATTA TGTCAAAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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hum-1 cDNA ORF clone, Caenorhabditis elegans

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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