BRAFLDRAFT_92981 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_92981
Entrez Gene ID	7245243
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002610214.1	XP_002610260.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_92981 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_92981 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb16920	XM_002610214.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_92981 ( XM_002610214.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002610260.1 Branchiostoma floridae hypothetical protein, mRNA.

Price & Availability↑

CloneID	OBb16920
Clone ID Related Accession (Same CDS sequence)	XM_002610214.1
Accession Version	XM_002610214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101543). COMPLETENESS: incomplete on both ends.

ATGCGGCTGT GTGGACAGGA GAACTCGCAG GGAGTGTACA CTTTGGACCG GAGAGGCCGG 
GATGCGATCG TGGCACTGGG AGTCTTCATC ATCGAGTCAG GACTTCAGCA CAAGGAGCTC 
CTAGTCCCCT ACCTGATCGA AATCCTTAGA AACCTCCCTG GTGCTCATTG GATCCCTTCC 
CCCACCACAA GTAAACAGAA TGGCCTTCCT GAGAGTGAGC AGTTCAGCTT CTGCCTGGTG 
ACTCTGTTGA CAGACATAGC ACACAGGGAT CCATCCTTCC GTGGGGAGAT CATCACAGCT 
CAGGTAGAAG TGCTGCAGAC CCTTGCCACC TTGTGCCAGA GTGCTGACAT CCCCAAAGTT 
GCCCTTTGCA GTATAGTTGT CCCCTCTCTG CTGGGAGTTG CTCGTGCGCT CGGTCGGTAC 
AGCACCGGCG ACATCGGCCT AATCTCCCAG CTGTTTCCCC GTGAGACTTT CTGCTCCCCT 
GCATCCACTC GAGTTAGCAC ACCTGCCACA CCAACCTTCC CTGTCCTGGG GCACAGCCGT 
CCAGTCAGCG CCTGTCTACA ACTTACACTC AACCTACAGG GACACTTTTT GCCCCCCTCT 
CCCAACCCCT CACCAACGCC CTCCCCACGA GGGTCAGAAG GTGCAGCCAT CTTTGGCAGC 
TCTCTGCCTA ACTCCCCCAT CCATGGAGGT GTATATCAGG GGGTGATGTC TCCACTGGCT 
TCAGAAGATC TGTTGCAAAC TGTTGGCACC AGCTTCCCCA TGGCGGCCAC GCTAGGAGGG 
ACAAGGAGAG CCCCTTTACA TCTCAATCCA GCCCAGTTAC AGGATATCCT GTCCACCGCT 
AAATCTCTCC TTACCAAAGA CATTCTCAAG AACTTGGATG CAAAAGCAAC AGAGTGCTTC 
ATGCCTGGGG GAGGCCCAAG CTACCCCTAT CATTCCTTCA GCGAGACTTT GACGGTTGTG 
TTGACCAATC TTCTTCACGA TCTTCTCTAT AATGTAAAGG ATCTTTCTGC CTCGTTTACC 
AAGGAAGTCC ATGACTTCAT CAAAGGTTTG TTCATTGCGG GACAAACGGA GGTCATGGAG 
AAACTAGCAG AAGCAAAACT TTCCTACAAG GCGTTCAACC CAGTGGTGCT GCATGTTAGA 
GCCAACGCTG CCTGTGTGGA CCTGCTGGTA TGGGCAGAGC AGGAGGAGGC AGGGGTGGAT 
GCCCTCTGTA GTCGTCTCCA GGAGAAACTT AACACAAGCA CAAGCTCCAA GCTGGTGGTA 
TTGCACATGC CACTGCTCCT GTGCTGCCTG CAGGGCCTCG GGTCCCTGGC AGAAAAGTTT 
CCCGGGAACA CGTACAACAT TCTCAACATT CTCCGCGACT TCCTGATCAA CCCTTCCCCC 
CTGGTGATGA AGCTTCACCG TCTGCAGGTC ACCACTGCCA CCAGGGGCAA TGCACACTCA 
CTCAGTGGGC CTCCATCCAT CCATGTTACC GGGACCAGTC CTGTTCACTC ACAGAGTCAG 
TCTTCTACCG AGTACCACAG CCTGCGCGAC TCAGCCATCA CCAACATCTG CCGTGTCCTA 
AAGGCGGGGA TAAAGGACGA TCCAGACTGT GTTCATGCGT TTCTGGCATC CCTGTCTAAC 
CGGCTGTATG CTGCCGAGAT AAGTGACAGG GAATCAACTC TCATCTCCAC CCACACTATT 
CACATCCTTG GTGCTATTGC AGTTACTTTC AAGGATGACC TCAAGACCAT GCAGTCAGTC 
CTTCAGATCT TCCAGCAGCG GTTCTGTAGC CCTGCCTCTC CTCTAGATGT CCTTATTGTG 
GACCAGATGG CTCTCATGCT CCTAGCAGGA GAACCTACCA CGTACCAGGA GATCCTGAAG 
ATGTTCATCA CAATCACTGT GGAGGCAGGG TCTGCCACGT ACTCACAGGA CCTTCATGCT 
ACCAACAACA AAGCCCATGG TTACAGGCAT GTTTCACTGG CAGTCATCAA TGCCCTGGGG 
GTTGTAGCTA GTAATTTACA GGGTGAGGAA CAGCTTCAGC AACTTCTTGT CCGTTTGCTG 
GAGCTGTTTG TGCAGATGGG GCTTGAAGGT AAGCGAGCGA GTGAGAAGGC CAGCGAGAAG 
TCCTCCACAG CAATCAAGGC TTCCAGCAGT GCAGGAAACT TGGGCATCCT CATTCCGATC 
CTGGCTTTGT TGATGAAACG CTTGACCACA ATCGTAGACC CCAATCCACG GCTGTACAAG 
CTGTTTCGTG ACTTCTGGCT GTACTGTGTG GTCATGGGAT TTGTCACAGA AGGTGCCGGC 
TTGTGGCCAC CGGAATGGTA TGATGGTGTT TGCGAGATCG CCACCAAGTC CCCTGTGTTG 
CTGTCTCCAG GCGGTGAGCA CCTACGGTCA GAGCTCCAGT ACAACTCAGC ACTGAGGAAT 
GATGCTGCAG GGCCAGCAGA GCTGTATGAA CTGAGGGCCA CAATCCTCAA CCTCCTGGGA 
CATCCAGCAG AGGTAGGGTC ACTCATTAAC AAGCTCAGCT TTGCCCAGTG CACCTACCTT 
CTCTCTGTGT TCAAGTTGGA AACTATGAGA GTCAAACACT CCACAGATAT GAGTAGCTTT 
CACCTCGTCT TCCGCTACCT AGAGGACAAA CAGGTGCAGA AGGACAAGGC AGGGATGCTG 
CAGTGTCTGC TTGCTGTTGC TGATCAGGCT TTCAAGATCT TCCTTGATGT CATGACCAAC 
AAGCCACGGA CAGATGTGTG GGAGAAAGAG CTTGTCAAGC ATGCCCAGTT CCTACTTGTC 
AAGTTCAACC ATCAGATGAA GCGCATCCGT CGTGTTGCAG ACAAGTTTTT GTCAGGGCTT 
GTTGACCGGT TTCCACACCT ACTGTGGAGC GGGGAGGTGA TCTCTACCAT GCTGGATATT 
CTACAGCTCC TGTATTTGCA AGAGGCACTG AAGTGGGCAC CTGCAGCCAC CAAGTCTCAC 
CTGGAGGAGT ACTTGAGAAA AATCGAAAAC ACCTGCGACG GACTGACCAA ACATGCGGGC 
ATGGCCCTTG CAGCAGAAAG CATTTTGCAA TACGCAGGTA ACACGAAGGA TGTGGCTACG 
TCAAAGATAG ACAAGAGACC AAAGTGTGTG AACAAGGATC ATTCCAGTTT TGTGGCTTCC 
ACCAACATGA GGAGCTGCTT CATGGGTGAG GTGCAAGGCA TGTTGAGTCT GTGTCAGGGC 
GATGAGAGGA GGCTGGCAAG GACCTTCCAC CAACAGCTCA CTGCATCCTC ACAAGGCAGG 
GACTTCCAGC ATTTCCGTAA GTCCCTCTTT AGAGTTACAG CTCTCCTCAT CAACAGCAAG 
GGCTGCCACC GGCCTCTGCT GCACAGCCTA TCCCAGGCAC CCATACGCTA TTTCACAGAC 
AAGGCCATGG AAACCACCAT CTGCTGTTGG CAGTGGTTGC TAGCGGCTAG AGCAGACTTG 
GAGCTTCCCT TCATGCTGGC CATGACAGCA GCCTGGAAGA TGGGAGTGGA CATGCGCCTC 
GGGCTGTTCA TGCACAACGT GGACGAGGTG GACGTGCTGG CTAGCTTTGG GGAGGAGGAC 
TGTAAGTCAA AGGCGCCATA TGTTGAGCCG CACAACATCT GGATCAACTT CCTGATGGAG 
AGGTTTGAGG TGGTGAAGTA CCGCAGCACC TCACAAGTGG AAATGTTTGC CGACCTGCTG 
CACTACTCCC TGCCGATGTT CGTGGGCGGA AAGGACCCCA TCATGAACCG ACATGTTGTT 
GGGGTTGGTG CCAGATTCCG GCTTCTCACC CTTGGTCTCC TGCTCCTCCA AGGCGATGTC 
CTGCCCAGCT GCCCCAGCAA GAATGTGCTG CGGGAGAAAG TCTACACCAC AGCATTTGAC 
TACTTCACAG CCGTTCTACC CATTCCACTC AAGGACACCT TGACATTGAG GGAGGTTGTG 
GAGATGGTCA TGAAGTTCTG GAATGCCACT GCAACCATGA CGTCCGTTCC TGCTGATCTG 
CGAGCAAGCT CTGACGTCGG CCCTCGTTCA ACTCAAGGTT GGGTAAACAC CATGCCAATG 
TCCTCCAACA TGTCTGCCCT GTCCAGGCGG TCCACAGGAA CCACCCGTAG CAAGAGCAGG 
GTGGGAGAGG TACTGGTGAA GGACTACATG CGGCGACGAG GGCTGCTGCT GTCCCTGGTG 
GGGAACGAGA TCGAGCGGCT AACGACGTGG TACAACCCGC TGGGTCTGTC GGAGCTGAGC 
TTCGTGGGGC AGGACACCAT CACGGCGTGG CAGTCCCAGC CCATGTCAGA CAGGGCCTGG 
AGGGACACGG TACAGATGGC GTGGGACATC TCCCCACGAC TAGCACTGCA GCTCCCAGCC 
AGGTTCCGTC AGACTGAAGC CATTTCCAGG GAGGTGACAC GTCTAGTCAG GAATGACCCC 
CAAGCAGTTG TAGATGTCCC GAGAGCAATC CAGTACCTGG TCACAGAACA CAGTGTTGAA 
GTGGATGCCC CAGAACTGAG CCACATCCTC ACCTGGACAG GTGTAAACCC TGCTATGGCG 
CTATCGTACT TCTCGCGCCA GTTCCCACCA CATCCATTCA CCTCGCAACT TGGCGTGAAG 
GTCCTGCGCT CCCTCCCACC CGAGGTCACC CTGTTCTACG TGCCACAGCT GATCCAGGCT 
CTGCGCTATG ACACCATGGG GTATGTACAG GAGTATATCC AGTGGGCCAG TCGGCGCTCC 
CAGCTCCTGG CTCACCAGTT TGTCTGGAAC ATGAAGACCA ACGCTTACAC TGATGAGGAT 
GGCACCATCA AGGACGCAGA GGTCGGAGAT CGTTTTGAGG CCATGATCGA ACTGATGACT 
CGGACATTGT CAGGGCCAGC CAAGGAATTC TATCAGCGGG AGTTTGACTT CTTTGGGAAG 
GTGACGGCCA TCTCAGGGGA GATCAGGCCC TATCCAAAAG GTGCAGAGAG GAAGAAGGCC 
TGTCTCAAGG CCATGAAAAA AATTGCTGTC CAGCCTGGCT GTTACCTACC CAGTAACCCT 
GAGGCTGTTG TGTTGGGAAT CGACTACAAC TCAGGAACTC CCCTACAAAG TGCAGCAAAG 
GCACCTTTCC TAGCCAAGTT TAAGGTTAGA AAGTGTGGGA TCAATGATCT GGAGAACCTT 
GGCCTGGAGG GAGGGGACAC AACAGATGGA ACAGCAGAGG AGGTCTGGCA GGCAGCCATC 
TTTAAAGTGG GAGATGATGT GCGGCAGGAC ATGCTTGCAT TGCAGGTGAT GACGCTGTTT 
AAGAACATAT TCCGGCAGGC GGGTCTGGAC CTGTTCCTGT ACCCGTACCG GGTCATCGCC 
ACCGCGCCGG GCTGTGGGGT CATCGAGTGC ATCCCCGACA CCAAGTCTCG CGACCAGCTG 
GGACGACAGA CCGACATTGG CCTTTACGAG TACTTCCTGC AGAAGTATGG GGATGAGATG 
ACCCTCATGT TTCAGAATGC CCGCCGGAAC TTCATTGAAT CCATGGCGGC TTACAGCGTT 
ATCGGGTTCC TGCTACAGAT CAAAGACAGG CATAACGGAA ACATCATGCT GGACTCACAG 
GGCCACATCA TGCACATTGA CTTCGGCTTC ATGTTTGAGA GCTCCCCGGG TGGAAACCTG 
GGATGGGAGC CAGACATCAA ACTGACCGAT GAGATGGTGA TGATCATGGG TGGGAAGATG 
GATGCCCCGC CATTCCGGCG CTTCATGGAG CTGTGTATAC AGGGCTACCT TGCTGTGCGC 
CCCTACCAGG AGGCCATTGT GTCACTGGTT GCATTAATGT TGGACACGGC ACTGCCATGC 
TTTCGAGGCC AGACCATCAA ACTGCTTCGC CAGCGCTTCG CTCCCCTGCA GAACGAGAGG 
GACGCGGCCA ACTACATGCA GAAGGTCATC AAGGACTCCT TCCTCAACTA CAGGAGTATC 
ACCTACGACA TGCTGCAGTA CTACCAAAAC CAGATCCCCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002610260.1
CDS	1..5925
Translation	MRLCGQENSQGVYTLDRRGRDAIVALGVFIIESGLQHKELLVPYLIEILRNLPGAHWIPSPTTSKQNGLPESEQFSFCLVTLLTDIAHRDPSFRGEIITAQVEVLQTLATLCQSADIPKVALCSIVVPSLLGVARALGRYSTGDIGLISQLFPRETFCSPASTRVSTPATPTFPVLGHSRPVSACLQLTLNLQGHFLPPSPNPSPTPSPRGSEGAAIFGSSLPNSPIHGGVYQGVMSPLASEDLLQTVGTSFPMAATLGGTRRAPLHLNPAQLQDILSTAKSLLTKDILKNLDAKATECFMPGGGPSYPYHSFSETLTVVLTNLLHDLLYNVKDLSASFTKEVHDFIKGLFIAGQTEVMEKLAEAKLSYKAFNPVVLHVRANAACVDLLVWAEQEEAGVDALCSRLQEKLNTSTSSKLVVLHMPLLLCCLQGLGSLAEKFPGNTYNILNILRDFLINPSPLVMKLHRLQVTTATRGNAHSLSGPPSIHVTGTSPVHSQSQSSTEYHSLRDSAITNICRVLKAGIKDDPDCVHAFLASLSNRLYAAEISDRESTLISTHTIHILGAIAVTFKDDLKTMQSVLQIFQQRFCSPASPLDVLIVDQMALMLLAGEPTTYQEILKMFITITVEAGSATYSQDLHATNNKAHGYRHVSLAVINALGVVASNLQGEEQLQQLLVRLLELFVQMGLEGKRASEKASEKSSTAIKASSSAGNLGILIPILALLMKRLTTIVDPNPRLYKLFRDFWLYCVVMGFVTEGAGLWPPEWYDGVCEIATKSPVLLSPGGEHLRSELQYNSALRNDAAGPAELYELRATILNLLGHPAEVGSLINKLSFAQCTYLLSVFKLETMRVKHSTDMSSFHLVFRYLEDKQVQKDKAGMLQCLLAVADQAFKIFLDVMTNKPRTDVWEKELVKHAQFLLVKFNHQMKRIRRVADKFLSGLVDRFPHLLWSGEVISTMLDILQLLYLQEALKWAPAATKSHLEEYLRKIENTCDGLTKHAGMALAAESILQYAGNTKDVATSKIDKRPKCVNKDHSSFVASTNMRSCFMGEVQGMLSLCQGDERRLARTFHQQLTASSQGRDFQHFRKSLFRVTALLINSKGCHRPLLHSLSQAPIRYFTDKAMETTICCWQWLLAARADLELPFMLAMTAAWKMGVDMRLGLFMHNVDEVDVLASFGEEDCKSKAPYVEPHNIWINFLMERFEVVKYRSTSQVEMFADLLHYSLPMFVGGKDPIMNRHVVGVGARFRLLTLGLLLLQGDVLPSCPSKNVLREKVYTTAFDYFTAVLPIPLKDTLTLREVVEMVMKFWNATATMTSVPADLRASSDVGPRSTQGWVNTMPMSSNMSALSRRSTGTTRSKSRVGEVLVKDYMRRRGLLLSLVGNEIERLTTWYNPLGLSELSFVGQDTITAWQSQPMSDRAWRDTVQMAWDISPRLALQLPARFRQTEAISREVTRLVRNDPQAVVDVPRAIQYLVTEHSVEVDAPELSHILTWTGVNPAMALSYFSRQFPPHPFTSQLGVKVLRSLPPEVTLFYVPQLIQALRYDTMGYVQEYIQWASRRSQLLAHQFVWNMKTNAYTDEDGTIKDAEVGDRFEAMIELMTRTLSGPAKEFYQREFDFFGKVTAISGEIRPYPKGAERKKACLKAMKKIAVQPGCYLPSNPEAVVLGIDYNSGTPLQSAAKAPFLAKFKVRKCGINDLENLGLEGGDTTDGTAEEVWQAAIFKVGDDVRQDMLALQVMTLFKNIFRQAGLDLFLYPYRVIATAPGCGVIECIPDTKSRDQLGRQTDIGLYEYFLQKYGDEMTLMFQNARRNFIESMAAYSVIGFLLQIKDRHNGNIMLDSQGHIMHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMDAPPFRRFMELCIQGYLAVRPYQEAIVSLVALMLDTALPCFRGQTIKLLRQRFAPLQNERDAANYMQKVIKDSFLNYRSITYDMLQYYQNQIPY

Target ORF information:

RefSeq Version	XM_002610214.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002610214.1

ORF Insert Sequence:

ATGCGGCTGT GTGGACAGGA GAACTCGCAG GGAGTGTACA CTTTGGACCG GAGAGGCCGG 
GATGCGATCG TGGCACTGGG AGTCTTCATC ATCGAGTCAG GACTTCAGCA CAAGGAGCTC 
CTAGTCCCCT ACCTGATCGA AATCCTTAGA AACCTCCCTG GTGCTCATTG GATCCCTTCC 
CCCACCACAA GTAAACAGAA TGGCCTTCCT GAGAGTGAGC AGTTCAGCTT CTGCCTGGTG 
ACTCTGTTGA CAGACATAGC ACACAGGGAT CCATCCTTCC GTGGGGAGAT CATCACAGCT 
CAGGTAGAAG TGCTGCAGAC CCTTGCCACC TTGTGCCAGA GTGCTGACAT CCCCAAAGTT 
GCCCTTTGCA GTATAGTTGT CCCCTCTCTG CTGGGAGTTG CTCGTGCGCT CGGTCGGTAC 
AGCACCGGCG ACATCGGCCT AATCTCCCAG CTGTTTCCCC GTGAGACTTT CTGCTCCCCT 
GCATCCACTC GAGTTAGCAC ACCTGCCACA CCAACCTTCC CTGTCCTGGG GCACAGCCGT 
CCAGTCAGCG CCTGTCTACA ACTTACACTC AACCTACAGG GACACTTTTT GCCCCCCTCT 
CCCAACCCCT CACCAACGCC CTCCCCACGA GGGTCAGAAG GTGCAGCCAT CTTTGGCAGC 
TCTCTGCCTA ACTCCCCCAT CCATGGAGGT GTATATCAGG GGGTGATGTC TCCACTGGCT 
TCAGAAGATC TGTTGCAAAC TGTTGGCACC AGCTTCCCCA TGGCGGCCAC GCTAGGAGGG 
ACAAGGAGAG CCCCTTTACA TCTCAATCCA GCCCAGTTAC AGGATATCCT GTCCACCGCT 
AAATCTCTCC TTACCAAAGA CATTCTCAAG AACTTGGATG CAAAAGCAAC AGAGTGCTTC 
ATGCCTGGGG GAGGCCCAAG CTACCCCTAT CATTCCTTCA GCGAGACTTT GACGGTTGTG 
TTGACCAATC TTCTTCACGA TCTTCTCTAT AATGTAAAGG ATCTTTCTGC CTCGTTTACC 
AAGGAAGTCC ATGACTTCAT CAAAGGTTTG TTCATTGCGG GACAAACGGA GGTCATGGAG 
AAACTAGCAG AAGCAAAACT TTCCTACAAG GCGTTCAACC CAGTGGTGCT GCATGTTAGA 
GCCAACGCTG CCTGTGTGGA CCTGCTGGTA TGGGCAGAGC AGGAGGAGGC AGGGGTGGAT 
GCCCTCTGTA GTCGTCTCCA GGAGAAACTT AACACAAGCA CAAGCTCCAA GCTGGTGGTA 
TTGCACATGC CACTGCTCCT GTGCTGCCTG CAGGGCCTCG GGTCCCTGGC AGAAAAGTTT 
CCCGGGAACA CGTACAACAT TCTCAACATT CTCCGCGACT TCCTGATCAA CCCTTCCCCC 
CTGGTGATGA AGCTTCACCG TCTGCAGGTC ACCACTGCCA CCAGGGGCAA TGCACACTCA 
CTCAGTGGGC CTCCATCCAT CCATGTTACC GGGACCAGTC CTGTTCACTC ACAGAGTCAG 
TCTTCTACCG AGTACCACAG CCTGCGCGAC TCAGCCATCA CCAACATCTG CCGTGTCCTA 
AAGGCGGGGA TAAAGGACGA TCCAGACTGT GTTCATGCGT TTCTGGCATC CCTGTCTAAC 
CGGCTGTATG CTGCCGAGAT AAGTGACAGG GAATCAACTC TCATCTCCAC CCACACTATT 
CACATCCTTG GTGCTATTGC AGTTACTTTC AAGGATGACC TCAAGACCAT GCAGTCAGTC 
CTTCAGATCT TCCAGCAGCG GTTCTGTAGC CCTGCCTCTC CTCTAGATGT CCTTATTGTG 
GACCAGATGG CTCTCATGCT CCTAGCAGGA GAACCTACCA CGTACCAGGA GATCCTGAAG 
ATGTTCATCA CAATCACTGT GGAGGCAGGG TCTGCCACGT ACTCACAGGA CCTTCATGCT 
ACCAACAACA AAGCCCATGG TTACAGGCAT GTTTCACTGG CAGTCATCAA TGCCCTGGGG 
GTTGTAGCTA GTAATTTACA GGGTGAGGAA CAGCTTCAGC AACTTCTTGT CCGTTTGCTG 
GAGCTGTTTG TGCAGATGGG GCTTGAAGGT AAGCGAGCGA GTGAGAAGGC CAGCGAGAAG 
TCCTCCACAG CAATCAAGGC TTCCAGCAGT GCAGGAAACT TGGGCATCCT CATTCCGATC 
CTGGCTTTGT TGATGAAACG CTTGACCACA ATCGTAGACC CCAATCCACG GCTGTACAAG 
CTGTTTCGTG ACTTCTGGCT GTACTGTGTG GTCATGGGAT TTGTCACAGA AGGTGCCGGC 
TTGTGGCCAC CGGAATGGTA TGATGGTGTT TGCGAGATCG CCACCAAGTC CCCTGTGTTG 
CTGTCTCCAG GCGGTGAGCA CCTACGGTCA GAGCTCCAGT ACAACTCAGC ACTGAGGAAT 
GATGCTGCAG GGCCAGCAGA GCTGTATGAA CTGAGGGCCA CAATCCTCAA CCTCCTGGGA 
CATCCAGCAG AGGTAGGGTC ACTCATTAAC AAGCTCAGCT TTGCCCAGTG CACCTACCTT 
CTCTCTGTGT TCAAGTTGGA AACTATGAGA GTCAAACACT CCACAGATAT GAGTAGCTTT 
CACCTCGTCT TCCGCTACCT AGAGGACAAA CAGGTGCAGA AGGACAAGGC AGGGATGCTG 
CAGTGTCTGC TTGCTGTTGC TGATCAGGCT TTCAAGATCT TCCTTGATGT CATGACCAAC 
AAGCCACGGA CAGATGTGTG GGAGAAAGAG CTTGTCAAGC ATGCCCAGTT CCTACTTGTC 
AAGTTCAACC ATCAGATGAA GCGCATCCGT CGTGTTGCAG ACAAGTTTTT GTCAGGGCTT 
GTTGACCGGT TTCCACACCT ACTGTGGAGC GGGGAGGTGA TCTCTACCAT GCTGGATATT 
CTACAGCTCC TGTATTTGCA AGAGGCACTG AAGTGGGCAC CTGCAGCCAC CAAGTCTCAC 
CTGGAGGAGT ACTTGAGAAA AATCGAAAAC ACCTGCGACG GACTGACCAA ACATGCGGGC 
ATGGCCCTTG CAGCAGAAAG CATTTTGCAA TACGCAGGTA ACACGAAGGA TGTGGCTACG 
TCAAAGATAG ACAAGAGACC AAAGTGTGTG AACAAGGATC ATTCCAGTTT TGTGGCTTCC 
ACCAACATGA GGAGCTGCTT CATGGGTGAG GTGCAAGGCA TGTTGAGTCT GTGTCAGGGC 
GATGAGAGGA GGCTGGCAAG GACCTTCCAC CAACAGCTCA CTGCATCCTC ACAAGGCAGG 
GACTTCCAGC ATTTCCGTAA GTCCCTCTTT AGAGTTACAG CTCTCCTCAT CAACAGCAAG 
GGCTGCCACC GGCCTCTGCT GCACAGCCTA TCCCAGGCAC CCATACGCTA TTTCACAGAC 
AAGGCCATGG AAACCACCAT CTGCTGTTGG CAGTGGTTGC TAGCGGCTAG AGCAGACTTG 
GAGCTTCCCT TCATGCTGGC CATGACAGCA GCCTGGAAGA TGGGAGTGGA CATGCGCCTC 
GGGCTGTTCA TGCACAACGT GGACGAGGTG GACGTGCTGG CTAGCTTTGG GGAGGAGGAC 
TGTAAGTCAA AGGCGCCATA TGTTGAGCCG CACAACATCT GGATCAACTT CCTGATGGAG 
AGGTTTGAGG TGGTGAAGTA CCGCAGCACC TCACAAGTGG AAATGTTTGC CGACCTGCTG 
CACTACTCCC TGCCGATGTT CGTGGGCGGA AAGGACCCCA TCATGAACCG ACATGTTGTT 
GGGGTTGGTG CCAGATTCCG GCTTCTCACC CTTGGTCTCC TGCTCCTCCA AGGCGATGTC 
CTGCCCAGCT GCCCCAGCAA GAATGTGCTG CGGGAGAAAG TCTACACCAC AGCATTTGAC 
TACTTCACAG CCGTTCTACC CATTCCACTC AAGGACACCT TGACATTGAG GGAGGTTGTG 
GAGATGGTCA TGAAGTTCTG GAATGCCACT GCAACCATGA CGTCCGTTCC TGCTGATCTG 
CGAGCAAGCT CTGACGTCGG CCCTCGTTCA ACTCAAGGTT GGGTAAACAC CATGCCAATG 
TCCTCCAACA TGTCTGCCCT GTCCAGGCGG TCCACAGGAA CCACCCGTAG CAAGAGCAGG 
GTGGGAGAGG TACTGGTGAA GGACTACATG CGGCGACGAG GGCTGCTGCT GTCCCTGGTG 
GGGAACGAGA TCGAGCGGCT AACGACGTGG TACAACCCGC TGGGTCTGTC GGAGCTGAGC 
TTCGTGGGGC AGGACACCAT CACGGCGTGG CAGTCCCAGC CCATGTCAGA CAGGGCCTGG 
AGGGACACGG TACAGATGGC GTGGGACATC TCCCCACGAC TAGCACTGCA GCTCCCAGCC 
AGGTTCCGTC AGACTGAAGC CATTTCCAGG GAGGTGACAC GTCTAGTCAG GAATGACCCC 
CAAGCAGTTG TAGATGTCCC GAGAGCAATC CAGTACCTGG TCACAGAACA CAGTGTTGAA 
GTGGATGCCC CAGAACTGAG CCACATCCTC ACCTGGACAG GTGTAAACCC TGCTATGGCG 
CTATCGTACT TCTCGCGCCA GTTCCCACCA CATCCATTCA CCTCGCAACT TGGCGTGAAG 
GTCCTGCGCT CCCTCCCACC CGAGGTCACC CTGTTCTACG TGCCACAGCT GATCCAGGCT 
CTGCGCTATG ACACCATGGG GTATGTACAG GAGTATATCC AGTGGGCCAG TCGGCGCTCC 
CAGCTCCTGG CTCACCAGTT TGTCTGGAAC ATGAAGACCA ACGCTTACAC TGATGAGGAT 
GGCACCATCA AGGACGCAGA GGTCGGAGAT CGTTTTGAGG CCATGATCGA ACTGATGACT 
CGGACATTGT CAGGGCCAGC CAAGGAATTC TATCAGCGGG AGTTTGACTT CTTTGGGAAG 
GTGACGGCCA TCTCAGGGGA GATCAGGCCC TATCCAAAAG GTGCAGAGAG GAAGAAGGCC 
TGTCTCAAGG CCATGAAAAA AATTGCTGTC CAGCCTGGCT GTTACCTACC CAGTAACCCT 
GAGGCTGTTG TGTTGGGAAT CGACTACAAC TCAGGAACTC CCCTACAAAG TGCAGCAAAG 
GCACCTTTCC TAGCCAAGTT TAAGGTTAGA AAGTGTGGGA TCAATGATCT GGAGAACCTT 
GGCCTGGAGG GAGGGGACAC AACAGATGGA ACAGCAGAGG AGGTCTGGCA GGCAGCCATC 
TTTAAAGTGG GAGATGATGT GCGGCAGGAC ATGCTTGCAT TGCAGGTGAT GACGCTGTTT 
AAGAACATAT TCCGGCAGGC GGGTCTGGAC CTGTTCCTGT ACCCGTACCG GGTCATCGCC 
ACCGCGCCGG GCTGTGGGGT CATCGAGTGC ATCCCCGACA CCAAGTCTCG CGACCAGCTG 
GGACGACAGA CCGACATTGG CCTTTACGAG TACTTCCTGC AGAAGTATGG GGATGAGATG 
ACCCTCATGT TTCAGAATGC CCGCCGGAAC TTCATTGAAT CCATGGCGGC TTACAGCGTT 
ATCGGGTTCC TGCTACAGAT CAAAGACAGG CATAACGGAA ACATCATGCT GGACTCACAG 
GGCCACATCA TGCACATTGA CTTCGGCTTC ATGTTTGAGA GCTCCCCGGG TGGAAACCTG 
GGATGGGAGC CAGACATCAA ACTGACCGAT GAGATGGTGA TGATCATGGG TGGGAAGATG 
GATGCCCCGC CATTCCGGCG CTTCATGGAG CTGTGTATAC AGGGCTACCT TGCTGTGCGC 
CCCTACCAGG AGGCCATTGT GTCACTGGTT GCATTAATGT TGGACACGGC ACTGCCATGC 
TTTCGAGGCC AGACCATCAA ACTGCTTCGC CAGCGCTTCG CTCCCCTGCA GAACGAGAGG 
GACGCGGCCA ACTACATGCA GAAGGTCATC AAGGACTCCT TCCTCAACTA CAGGAGTATC 
ACCTACGACA TGCTGCAGTA CTACCAAAAC CAGATCCCCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_92981 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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