Bchhk5 cDNA ORF clone, Botrytis cinerea B05.10

Gene
Clones
gRNAs

Summary

Gene Symbol	Bchhk5
Entrez Gene ID	5433621
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Botrytis cinerea B05.10

mRNA and Protein(s)

mRNA	Protein	Name
XM_001553044.2	XP_001553094.2	Bchhk5
XM_001553044.1	XP_001553094.1	hypothetical protein
XM_024697058.1	XP_024552872.1	Bchhk5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A gapless genome sequence of the fungus Botrytis cinerea.
Van Kan JA, Stassen JH, Mosbach A, Van Der Lee TA, Faino L, Farmer AD, Papasotiriou DG, Zhou S, Seidl MF, Cottam E, Edel D, Hahn M, Schwartz DC, Dietrich RA, Widdison S, Scalliet G
Molecular plant pathology18(1)75-89(2017 01)

Genome update of Botrytis cinerea strains B05.10 and T4.
Staats M, van Kan JA
Eukaryotic cell11(11)1413-4(2012 Nov)

Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
Amselem J, Cuomo CA, van Kan JA, Viaud M, Benito EP, Couloux A, Coutinho PM, de Vries RP, Dyer PS, Fillinger S, Fournier E, Gout L, Hahn M, Kohn L, Lapalu N, Plummer KM, Pradier JM, Quévillon E, Sharon A, Simon A, ten Have A, Tudzynski B, Tudzynski P, Wincker P, Andrew M, Anthouard V, Beever RE, Beffa R, Benoit I, Bouzid O, Brault B, Chen Z, Choquer M, Collémare J, Cotton P, Danchin EG, Da Silva C, Gautier A, Giraud C, Giraud T, Gonzalez C, Grossetete S, Güldener U, Henrissat B, Howlett BJ, Kodira C, Kretschmer M, Lappartient A, Leroch M, Levis C, Mauceli E, Neuvéglise C, Oeser B, Pearson M, Poulain J, Poussereau N, Quesneville H, Rascle C, Schumacher J, Ségurens B, Sexton A, Silva E, Sirven C, Soanes DM, Talbot NJ, Templeton M, Yandava C, Yarden O, Zeng Q, Rollins JA, Lebrun MH, Dickman M
PLoS genetics7(8)e1002230(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bchhk5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bchhk5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBc12269	XM_001553044.1 Latest version!	Botryotinia fuckeliana B05.10 conserved hypothetical protein (BC1G_08461) partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBc23474	XM_001553044.2 Latest version!	Botrytis cinerea B05.10 Bchhk5 (Bchhk5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBc23475	XM_024697058.1 Latest version!	Botrytis cinerea B05.10 Bchhk5 (Bchhk5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bchhk5 ( XM_001553044.1 ) cDNA ORF clone, Botrytis cinerea B05.10 -> XP_001553094.1 Botryotinia fuckeliana B05.10 conserved hypothetical protein (BC1G_08461) partial mRNA.

Price & Availability↑

CloneID	OBc12269
Clone ID Related Accession (Same CDS sequence)	XM_001553044.1
Accession Version	XM_001553044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1204041600000
Organism	Botrytis cinerea B05.10
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001814523). COMPLETENESS: incomplete on both ends.

ATGAGGATTG GTATCAGGCT GCAACTTGGC CTGGTTGTCC TGGTGGCTGC TCTTGTTCCA 
TTGATTGTGC TCGCTCTCGC TACCTGGTTC AACAATTATA GTTTTACAGT CGACGTCAAA 
GAAAATGCAC TTAGCCTTAC GGCATCACTC AAAGCCGCTT CAGTGGCTTC CGATCTACTC 
CTAATCCAGG CCACATGCTC AACTATCGTC ACGAGGATCC TTATCCAGGA GGCCATCCGC 
GACTTTTATA GAGGAGCACC CGAGTGGAAC GAAGCAAGAG AAGATGTACA AAGCGCTTTG 
GCTTCTGGTG GTCTATCTGC ATTGCTTCAA GTAATCATAT TTTCGCGGAA TGAGACGGGC 
TCGGTCAATG GTATATTAAA TGTCACAGCC AATGCATCCG ATATACAACT GCCCTATAAT 
AACTCGCTCA ACCAGCCCGC TTTCCTAGGC GATGCTGATC AGGGATTTCC AGCGGCTTTG 
TATCCGAATA TCTCTTATAT CCCAACCACG GATCCCGACC CAAATGCTCC AGGAACGAAT 
TTTACACAGG TGTACGCATT TGCCGATTAC CCACTGAATA AAAGTGCTGC TCTTCTACTG 
GGGCCGTTAC AAATTAATAG CACTTATGCT TTAGTATCAC TGACCTTACC GATTGTGGAG 
AATTCGAATC CCGATAACAT TCTAGGTTAT ATGACTGTGG TAGCAGCTGC GACTTCGCTG 
ATTGACGTTG TTTATTCTCG GGAGGGCTTG GATAAAACAG GAATAGTGTT GGTAGTGGGA 
ACCAATCGAC GAGAGAACCA GTTCTCGTAC ATGTCGAGAC CGGCTACAGC AACTTACGAA 
CCCGACCTAC ATAATTTGAC AAAAGCAAGT GTCAAATACA TCTTCCCGCC ATACCCTCTC 
GAAGGGCAAA CTGATAGACA TTCCGTCTAC AATGCAAATT TAACTGAATA TGGGAGCTCA 
AATTTTAGCG AAGGCAATTA TCCGGCTATT CTACATGGAT TCGGACGACA GAATCCGAAA 
GTCAATAATG CCAGTAGTTA TATTACAACC ACAAACGAGC AAGGAGTAAA GGTGTCGGTA 
GGCTGGGCAA GACCACAAAG TGCACTGGTT GATTGGCTTT TGATCGTAGA ACAAGCTCAT 
TCCGAAGCTT GGGAACCCAT TATCAAGCTT AGGAATATCT TACTTGCATG TGTTTTTGGT 
ACATTTGGGT TGATCCTTAT TGTAGTTATA CCGATGGCTC ATTTCAGTGT TCGACCAATC 
AGAAGGCTAA GAGATGCCAC GAAACAATCC ATTCAGCCAC CGGGCTACAC TCCGCGTCAA 
AGTATAGAGG ATGAATTATA TGGCGAGGAA GAAGTCGGCG AGGGTAGTTC AAGTGGGTAC 
AAGAAAACGG GATTTTTGGT CAGGCTGAGA AAATTAACAC AGTCGGGGCG TCGTGTGTCG 
AGAGCAGAAA AATCAGAGAT CGACAGACGG AGAGATTTTA AAATTCCTGG AAAGGTGCCT 
GATCGAAAAC ACTGGATTGA GGACGAACTC ACGGATTTAA CATCGAAATT TAATGAAATG 
AGCGACGAAT TACTGCTGCA ATACACGAGT CTCGAATCCA AAGTTGCTGA ACGTACGGAA 
CAACTTGAAC ATTCCAAGAA GGCAGCGGAG GCAGCAAACG AGAGCAAAAC ATTATTCATT 
GCAAACATAT CGCACGAATT GAAAACTCCA TTGAACGGAA TTCTGGGAAT GTGTGCCGTA 
TGCATGGGTG AGGATGATTT GCCAAGCATC AAACGTTCAT TGAAGACGGT TTACAAATCT 
GGTGACCTTT TGTTGAATTT ATTGAATGAT CTTTTGACGT TCAGTAAGAA CCAGATTGGC 
CAGCAATTGC ATCTAGAAGA GAAAGAGTTT CGGCTGTCTG ATGTAAAGGA GCAGATCAGG 
AACATATTCC GAAAGCAAGT GGACGAAGGC GGTATTACTT TTGGAGTCCA CTTCCTGGGC 
ACTGAAAGCA CAGATGGAGC TCCACTAGAG AAACCGAGCG GAAAGCCTCT ACCAGCCCTA 
GGACCTAATG GTATTGGTAG ATTGAAAGAT ATGTGTTTGT GGGGTGATCA GCACAGAATT 
CTCCAAATCA TAATCAATCT AGTCAGCAAC AGTCTCAAAT TCACACCACC AGGAGGAAAG 
GTAGAAGTCA GAATTAGATG TATGGGCGAA TTGGAACAAC CATTGGATGG TGTCAATAGC 
CGAAATTCCA TGGGTTCGAA GCAGAGCTCT CACAGGAGAA ATGTTAGAAC CCGTACTGGC 
TCAGGATCGA ATGCTTCAGG GCATACTTCG ACGCATGCAT CGGGGCAAAT TTCAAGGACA 
GCTTCAAATA CCAAGCAAGC TGGGACTGCA CTAGTAATCA ATCCTATGGA TCCAAGGGCT 
CGTGTCCAAT TAAACGAGCG ATCGGCAACT CCGCCACCAA ATAATGCTAG GACTCTATGG 
TTTTCAATAG AGGTTGAGGA TACTGGACCC GGTATTCCCG AGCATCTACA GGAGCGAGTT 
TTCGAGCCTT TCGTACAGGC TGATCTTGGG TTGAATAGGA AATATGGAGG AACCGGATTG 
GGATTAAGTA TTTGCTCCCA GTTAGCAGGC CTTATGGGAG GTTCTATCTC TCTCGTTAGT 
ACACTTGGAG AAGGTTCCAC GTTCAATGTT CAGTTGCCAT TGAAATTCGT CAAGGAGCGA 
GCACCAAGTA CATCTAGTTC CGATGTTATT GGTTCTCGGC CCGCAAGCAT GGTTTCGCAA 
TCAGAACTCG AACGTCATAC TCCAAAGGGT TCAATTGATG CAACGACTGC TGTAGGATCC 
CCTGGGAAAA GCAATGGTGG TGCAGGCTAT TCAATAGATA CTCAACCTCG GTTGGTGGGA 
CTTCGACAGC CATTCTTCAC ATCATCCCCT ACACCTCCAC CCAATGACGA TAACGCAAAA 
ACTCAACTCG CTGCAATCGA TCGCGCTGCC AAGGAGAAAG TAGGGGATAA GAAAATTCGA 
GTTCTGGTTG CCGAAGATAA TCTTGTTAAT CAAGAGGTGG TTCTTCGCAT GCTTAAACTT 
GAAGACGTGT ATGACGTGGT GGTCGCCAAG GATGGGCAGG AGGCATACGA TATAGTAAAG 
CAAAGCATGG AAGAGGGCAA ATTCTTCAAC TTGATCTTTA TGGATATTCA AATGCCAAAT 
CTGGATGGGC TACAAAGTAC GAGATTAATC AGAGAAATGG GTTATTCTGC GGCCATTGTA 
GCACTAACGG CTTTTGCCGA AGAGAGTAAT GTCAAAGAAT GCTATGAGTC CGGAATGGAT 
CATTTCCTAA GTAAACCTAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001553094.1
CDS	1..3324
Translation	MRIGIRLQLGLVVLVAALVPLIVLALATWFNNYSFTVDVKENALSLTASLKAASVASDLLLIQATCSTIVTRILIQEAIRDFYRGAPEWNEAREDVQSALASGGLSALLQVIIFSRNETGSVNGILNVTANASDIQLPYNNSLNQPAFLGDADQGFPAALYPNISYIPTTDPDPNAPGTNFTQVYAFADYPLNKSAALLLGPLQINSTYALVSLTLPIVENSNPDNILGYMTVVAAATSLIDVVYSREGLDKTGIVLVVGTNRRENQFSYMSRPATATYEPDLHNLTKASVKYIFPPYPLEGQTDRHSVYNANLTEYGSSNFSEGNYPAILHGFGRQNPKVNNASSYITTTNEQGVKVSVGWARPQSALVDWLLIVEQAHSEAWEPIIKLRNILLACVFGTFGLILIVVIPMAHFSVRPIRRLRDATKQSIQPPGYTPRQSIEDELYGEEEVGEGSSSGYKKTGFLVRLRKLTQSGRRVSRAEKSEIDRRRDFKIPGKVPDRKHWIEDELTDLTSKFNEMSDELLLQYTSLESKVAERTEQLEHSKKAAEAANESKTLFIANISHELKTPLNGILGMCAVCMGEDDLPSIKRSLKTVYKSGDLLLNLLNDLLTFSKNQIGQQLHLEEKEFRLSDVKEQIRNIFRKQVDEGGITFGVHFLGTESTDGAPLEKPSGKPLPALGPNGIGRLKDMCLWGDQHRILQIIINLVSNSLKFTPPGGKVEVRIRCMGELEQPLDGVNSRNSMGSKQSSHRRNVRTRTGSGSNASGHTSTHASGQISRTASNTKQAGTALVINPMDPRARVQLNERSATPPPNNARTLWFSIEVEDTGPGIPEHLQERVFEPFVQADLGLNRKYGGTGLGLSICSQLAGLMGGSISLVSTLGEGSTFNVQLPLKFVKERAPSTSSSDVIGSRPASMVSQSELERHTPKGSIDATTAVGSPGKSNGGAGYSIDTQPRLVGLRQPFFTSSPTPPPNDDNAKTQLAAIDRAAKEKVGDKKIRVLVAEDNLVNQEVVLRMLKLEDVYDVVVAKDGQEAYDIVKQSMEEGKFFNLIFMDIQMPNLDGLQSTRLIREMGYSAAIVALTAFAEESNVKECYESGMDHFLSKPI

Target ORF information:

RefSeq Version	XM_001553044.1
Organism	Botrytis cinerea B05.10
Definition	Botryotinia fuckeliana B05.10 conserved hypothetical protein (BC1G_08461) partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001553044.1

ORF Insert Sequence:

ATGAGGATTG GTATCAGGCT GCAACTTGGC CTGGTTGTCC TGGTGGCTGC TCTTGTTCCA 
TTGATTGTGC TCGCTCTCGC TACCTGGTTC AACAATTATA GTTTTACAGT CGACGTCAAA 
GAAAATGCAC TTAGCCTTAC GGCATCACTC AAAGCCGCTT CAGTGGCTTC CGATCTACTC 
CTAATCCAGG CCACATGCTC AACTATCGTC ACGAGGATCC TTATCCAGGA GGCCATCCGC 
GACTTTTATA GAGGAGCACC CGAGTGGAAC GAAGCAAGAG AAGATGTACA AAGCGCTTTG 
GCTTCTGGTG GTCTATCTGC ATTGCTTCAA GTAATCATAT TTTCGCGGAA TGAGACGGGC 
TCGGTCAATG GTATATTAAA TGTCACAGCC AATGCATCCG ATATACAACT GCCCTATAAT 
AACTCGCTCA ACCAGCCCGC TTTCCTAGGC GATGCTGATC AGGGATTTCC AGCGGCTTTG 
TATCCGAATA TCTCTTATAT CCCAACCACG GATCCCGACC CAAATGCTCC AGGAACGAAT 
TTTACACAGG TGTACGCATT TGCCGATTAC CCACTGAATA AAAGTGCTGC TCTTCTACTG 
GGGCCGTTAC AAATTAATAG CACTTATGCT TTAGTATCAC TGACCTTACC GATTGTGGAG 
AATTCGAATC CCGATAACAT TCTAGGTTAT ATGACTGTGG TAGCAGCTGC GACTTCGCTG 
ATTGACGTTG TTTATTCTCG GGAGGGCTTG GATAAAACAG GAATAGTGTT GGTAGTGGGA 
ACCAATCGAC GAGAGAACCA GTTCTCGTAC ATGTCGAGAC CGGCTACAGC AACTTACGAA 
CCCGACCTAC ATAATTTGAC AAAAGCAAGT GTCAAATACA TCTTCCCGCC ATACCCTCTC 
GAAGGGCAAA CTGATAGACA TTCCGTCTAC AATGCAAATT TAACTGAATA TGGGAGCTCA 
AATTTTAGCG AAGGCAATTA TCCGGCTATT CTACATGGAT TCGGACGACA GAATCCGAAA 
GTCAATAATG CCAGTAGTTA TATTACAACC ACAAACGAGC AAGGAGTAAA GGTGTCGGTA 
GGCTGGGCAA GACCACAAAG TGCACTGGTT GATTGGCTTT TGATCGTAGA ACAAGCTCAT 
TCCGAAGCTT GGGAACCCAT TATCAAGCTT AGGAATATCT TACTTGCATG TGTTTTTGGT 
ACATTTGGGT TGATCCTTAT TGTAGTTATA CCGATGGCTC ATTTCAGTGT TCGACCAATC 
AGAAGGCTAA GAGATGCCAC GAAACAATCC ATTCAGCCAC CGGGCTACAC TCCGCGTCAA 
AGTATAGAGG ATGAATTATA TGGCGAGGAA GAAGTCGGCG AGGGTAGTTC AAGTGGGTAC 
AAGAAAACGG GATTTTTGGT CAGGCTGAGA AAATTAACAC AGTCGGGGCG TCGTGTGTCG 
AGAGCAGAAA AATCAGAGAT CGACAGACGG AGAGATTTTA AAATTCCTGG AAAGGTGCCT 
GATCGAAAAC ACTGGATTGA GGACGAACTC ACGGATTTAA CATCGAAATT TAATGAAATG 
AGCGACGAAT TACTGCTGCA ATACACGAGT CTCGAATCCA AAGTTGCTGA ACGTACGGAA 
CAACTTGAAC ATTCCAAGAA GGCAGCGGAG GCAGCAAACG AGAGCAAAAC ATTATTCATT 
GCAAACATAT CGCACGAATT GAAAACTCCA TTGAACGGAA TTCTGGGAAT GTGTGCCGTA 
TGCATGGGTG AGGATGATTT GCCAAGCATC AAACGTTCAT TGAAGACGGT TTACAAATCT 
GGTGACCTTT TGTTGAATTT ATTGAATGAT CTTTTGACGT TCAGTAAGAA CCAGATTGGC 
CAGCAATTGC ATCTAGAAGA GAAAGAGTTT CGGCTGTCTG ATGTAAAGGA GCAGATCAGG 
AACATATTCC GAAAGCAAGT GGACGAAGGC GGTATTACTT TTGGAGTCCA CTTCCTGGGC 
ACTGAAAGCA CAGATGGAGC TCCACTAGAG AAACCGAGCG GAAAGCCTCT ACCAGCCCTA 
GGACCTAATG GTATTGGTAG ATTGAAAGAT ATGTGTTTGT GGGGTGATCA GCACAGAATT 
CTCCAAATCA TAATCAATCT AGTCAGCAAC AGTCTCAAAT TCACACCACC AGGAGGAAAG 
GTAGAAGTCA GAATTAGATG TATGGGCGAA TTGGAACAAC CATTGGATGG TGTCAATAGC 
CGAAATTCCA TGGGTTCGAA GCAGAGCTCT CACAGGAGAA ATGTTAGAAC CCGTACTGGC 
TCAGGATCGA ATGCTTCAGG GCATACTTCG ACGCATGCAT CGGGGCAAAT TTCAAGGACA 
GCTTCAAATA CCAAGCAAGC TGGGACTGCA CTAGTAATCA ATCCTATGGA TCCAAGGGCT 
CGTGTCCAAT TAAACGAGCG ATCGGCAACT CCGCCACCAA ATAATGCTAG GACTCTATGG 
TTTTCAATAG AGGTTGAGGA TACTGGACCC GGTATTCCCG AGCATCTACA GGAGCGAGTT 
TTCGAGCCTT TCGTACAGGC TGATCTTGGG TTGAATAGGA AATATGGAGG AACCGGATTG 
GGATTAAGTA TTTGCTCCCA GTTAGCAGGC CTTATGGGAG GTTCTATCTC TCTCGTTAGT 
ACACTTGGAG AAGGTTCCAC GTTCAATGTT CAGTTGCCAT TGAAATTCGT CAAGGAGCGA 
GCACCAAGTA CATCTAGTTC CGATGTTATT GGTTCTCGGC CCGCAAGCAT GGTTTCGCAA 
TCAGAACTCG AACGTCATAC TCCAAAGGGT TCAATTGATG CAACGACTGC TGTAGGATCC 
CCTGGGAAAA GCAATGGTGG TGCAGGCTAT TCAATAGATA CTCAACCTCG GTTGGTGGGA 
CTTCGACAGC CATTCTTCAC ATCATCCCCT ACACCTCCAC CCAATGACGA TAACGCAAAA 
ACTCAACTCG CTGCAATCGA TCGCGCTGCC AAGGAGAAAG TAGGGGATAA GAAAATTCGA 
GTTCTGGTTG CCGAAGATAA TCTTGTTAAT CAAGAGGTGG TTCTTCGCAT GCTTAAACTT 
GAAGACGTGT ATGACGTGGT GGTCGCCAAG GATGGGCAGG AGGCATACGA TATAGTAAAG 
CAAAGCATGG AAGAGGGCAA ATTCTTCAAC TTGATCTTTA TGGATATTCA AATGCCAAAT 
CTGGATGGGC TACAAAGTAC GAGATTAATC AGAGAAATGG GTTATTCTGC GGCCATTGTA 
GCACTAACGG CTTTTGCCGA AGAGAGTAAT GTCAAAGAAT GCTATGAGTC CGGAATGGAT 
CATTTCCTAA GTAAACCTAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bchhk5 ( XM_001553044.2 ) cDNA ORF clone, Botrytis cinerea B05.10 -> XP_001553094.2 Botrytis cinerea B05.10 Bchhk5 (Bchhk5), mRNA.

Price & Availability↑

CloneID	OBc23474
Clone ID Related Accession (Same CDS sequence)	XM_001553044.2
Accession Version	XM_001553044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523808000000
Organism	Botrytis cinerea B05.10
Product	Bchhk5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_037323). On Apr 17, 2018 this sequence version replaced XM_001553044.1.

ATGAGGATTG GTATCAGGCT GCAACTTGGC CTGGTTGTCC TGGTGGCTGC TCTTGTTCCA 
TTGATTGTGC TCGCTCTCGC TACCTGGTTC AACAATTATA GTTTTACAGT CGACGTCAAA 
GAAAATGCAC TTAGCCTTAC GGCATCACTC AAAGCCGCTT CAGTGGCTTC CGATCTACTC 
CTAATCCAGG CCACATGCTC AACTATCGTC ACGAGGATCC TTATCCAGGA GGCCATCCGC 
GACTTTTATA GAGGAGCACC CGAGTGGAAC GAAGCAAGAG AAGATGTACA AAGCGCTTTG 
GCTTCTGGTG GTCTATCTGC ATTGCTTCAA GTAATCATAT TTTCGCGGAA TGAGACGGGC 
TCGGTCAATG GTATATTAAA TGTCACAGCC AATGCATCCG ATATACAACT GCCCTATAAT 
AACTCGCTCA ACCAGCCCGC TTTCCTAGGC GATGCTGATC AGGGATTTCC AGCGGCTTTG 
TATCCGAATA TCTCTTATAT CCCAACCACG GATCCCGACC CAAATGCTCC AGGAACGAAT 
TTTACACAGG TGTACGCATT TGCCGATTAC CCACTGAATA AAAGTGCTGC TCTTCTACTG 
GGGCCGTTAC AAATTAATAG CACTTATGCT TTAGTATCAC TGACCTTACC GATTGTGGAG 
AATTCGAATC CCGATAACAT TCTAGGTTAT ATGACTGTGG TAGCAGCTGC GACTTCGCTG 
ATTGACGTTG TTTATTCTCG GGAGGGCTTG GATAAAACAG GAATAGTGTT GGTAGTGGGA 
ACCAATCGAC GAGAGAACCA GTTCTCGTAC ATGTCGAGAC CGGCTACAGC AACTTACGAA 
CCCGACCTAC ATAATTTGAC AAAAGCAAGT GTCAAATACA TCTTCCCGCC ATACCCTCTC 
GAAGGGCAAA CTGATAGACA TTCCGTCTAC AATGCAAATT TAACTGAATA TGGGAGCTCA 
AATTTTAGCG AAGGCAATTA TCCGGCTATT CTACATGGAT TCGGACGACA GAATCCGAAA 
GTCAATAATG CCAGTAGTTA TATTACAACC ACAAACGAGC AAGGAGTAAA GGTGTCGGTA 
GGCTGGGCAA GACCACAAAG TGCACTGGTT GATTGGCTTT TGATCGTAGA ACAAGCTCAT 
TCCGAAGCTT GGGAACCCAT TATCAAGCTT AGGAATATCT TACTTGCATG TGTTTTTGGT 
ACATTTGGGT TGATCCTTAT TGTAGTTATA CCGATGGCTC ATTTCAGTGT TCGACCAATC 
AGAAGGCTAA GAGATGCCAC GAAACAATCC ATTCAGCCAC CGGGCTACAC TCCGCGTCAA 
AGTATAGAGG ATGAATTATA TGGCGAGGAA GAAGTCGGCG AGGGTAGTTC AAGTGGGTAC 
AAGAAAACGG GATTTTTGGT CAGGCTGAGA AAATTAACAC AGTCGGGGCG TCGTGTGTCG 
AGAGCAGAAA AATCAGAGAT CGACAGACGG AGAGATTTTA AAATTCCTGG AAAGGTGCCT 
GATCGAAAAC ACTGGATTGA GGACGAACTC ACGGATTTAA CATCGAAATT TAATGAAATG 
AGCGACGAAT TACTGCTGCA ATACACGAGT CTCGAATCCA AAGTTGCTGA ACGTACGGAA 
CAACTTGAAC ATTCCAAGAA GGCAGCGGAG GCAGCAAACG AGAGCAAAAC ATTATTCATT 
GCAAACATAT CGCACGAATT GAAAACTCCA TTGAACGGAA TTCTGGGAAT GTGTGCCGTA 
TGCATGGGTG AGGATGATTT GCCAAGCATC AAACGTTCAT TGAAGACGGT TTACAAATCT 
GGTGACCTTT TGTTGAATTT ATTGAATGAT CTTTTGACGT TCAGTAAGAA CCAGATTGGC 
CAGCAATTGC ATCTAGAAGA GAAAGAGTTT CGGCTGTCTG ATGTAAAGGA GCAGATCAGG 
AACATATTCC GAAAGCAAGT GGACGAAGGC GGTATTACTT TTGGAGTCCA CTTCCTGGGC 
ACTGAAAGCA CAGATGGAGC TCCACTAGAG AAACCGAGCG GAAAGCCTCT ACCAGCCCTA 
GGACCTAATG GTATTGGTAG ATTGAAAGAT ATGTGTTTGT GGGGTGATCA GCACAGAATT 
CTCCAAATCA TAATCAATCT AGTCAGCAAC AGTCTCAAAT TCACACCACC AGGAGGAAAG 
GTAGAAGTCA GAATTAGATG TATGGGCGAA TTGGAACAAC CATTGGATGG TGTCAATAGC 
CGAAATTCCA TGGGTTCGAA GCAGAGCTCT CACAGGAGAA ATGTTAGAAC CCGTACTGGC 
TCAGGATCGA ATGCTTCAGG GCATACTTCG ACGCATGCAT CGGGGCAAAT TTCAAGGACA 
GCTTCAAATA CCAAGCAAGC TGGGACTGCA CTAGTAATCA ATCCTATGGA TCCAAGGGCT 
CGTGTCCAAT TAAACGAGCG ATCGGCAACT CCGCCACCAA ATAATGCTAG GACTCTATGG 
TTTTCAATAG AGGTTGAGGA TACTGGACCC GGTATTCCCG AGCATCTACA GGAGCGAGTT 
TTCGAGCCTT TCGTACAGGC TGATCTTGGG TTGAATAGGA AATATGGAGG AACCGGATTG 
GGATTAAGTA TTTGCTCCCA GTTAGCAGGC CTTATGGGAG GTTCTATCTC TCTCGTTAGT 
ACACTTGGAG AAGGTTCCAC GTTCAATGTT CAGTTGCCAT TGAAATTCGT CAAGGAGCGA 
GCACCAAGTA CATCTAGTTC CGATGTTATT GGTTCTCGGC CCGCAAGCAT GGTTTCGCAA 
TCAGAACTCG AACGTCATAC TCCAAAGGGT TCAATTGATG CAACGACTGC TGTAGGATCC 
CCTGGGAAAA GCAATGGTGG TGCAGGCTAT TCAATAGATA CTCAACCTCG GTTGGTGGGA 
CTTCGACAGC CATTCTTCAC ATCATCCCCT ACACCTCCAC CCAATGACGA TAACGCAAAA 
ACTCAACTCG CTGCAATCGA TCGCGCTGCC AAGGAGAAAG TAGGGGATAA GAAAATTCGA 
GTTCTGGTTG CCGAAGATAA TCTTGTTAAT CAAGAGGTGG TTCTTCGCAT GCTTAAACTT 
GAAGACGTGT ATGACGTGGT GGTCGCCAAG GATGGGCAGG AGGCATACGA TATAGTAAAG 
CAAAGCATGG AAGAGGGCAA ATTCTTCAAC TTGATCTTTA TGGATATTCA AATGCCAAAT 
CTGGATGGGC TACAAAGTAC GAGATTAATC AGAGAAATGG GTTATTCTGC GCCCATTGTA 
GCACTAACGG CTTTTGCCGA AGAGAGTAAT GTCAAAGAAT GCTATGAGTC CGGAATGGAT 
CATTTCCTAA GTAAACCTAT TCGACGACCA GCTCTCAAGC AAGTTCTTAA GAAATTCGCC 
ACTATCCCAG AGGAGATAGA AGACTCCTCC ATAACTCGAA AAAGCACATC AGAAAAAGGG 
CAAGCATCGA AACAGCAAAA CAAAAATACA TCTACTTCAA CATTCAGTCC GGCATCAAAT 
TCTGATGCTG ACCTCATTAA AATGAATGGG ACCCCTTCAC CTCCATCACC TTTAACCTCT 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001553094.2
CDS	785..4330
Translation	MRIGIRLQLGLVVLVAALVPLIVLALATWFNNYSFTVDVKENALSLTASLKAASVASDLLLIQATCSTIVTRILIQEAIRDFYRGAPEWNEAREDVQSALASGGLSALLQVIIFSRNETGSVNGILNVTANASDIQLPYNNSLNQPAFLGDADQGFPAALYPNISYIPTTDPDPNAPGTNFTQVYAFADYPLNKSAALLLGPLQINSTYALVSLTLPIVENSNPDNILGYMTVVAAATSLIDVVYSREGLDKTGIVLVVGTNRRENQFSYMSRPATATYEPDLHNLTKASVKYIFPPYPLEGQTDRHSVYNANLTEYGSSNFSEGNYPAILHGFGRQNPKVNNASSYITTTNEQGVKVSVGWARPQSALVDWLLIVEQAHSEAWEPIIKLRNILLACVFGTFGLILIVVIPMAHFSVRPIRRLRDATKQSIQPPGYTPRQSIEDELYGEEEVGEGSSSGYKKTGFLVRLRKLTQSGRRVSRAEKSEIDRRRDFKIPGKVPDRKHWIEDELTDLTSKFNEMSDELLLQYTSLESKVAERTEQLEHSKKAAEAANESKTLFIANISHELKTPLNGILGMCAVCMGEDDLPSIKRSLKTVYKSGDLLLNLLNDLLTFSKNQIGQQLHLEEKEFRLSDVKEQIRNIFRKQVDEGGITFGVHFLGTESTDGAPLEKPSGKPLPALGPNGIGRLKDMCLWGDQHRILQIIINLVSNSLKFTPPGGKVEVRIRCMGELEQPLDGVNSRNSMGSKQSSHRRNVRTRTGSGSNASGHTSTHASGQISRTASNTKQAGTALVINPMDPRARVQLNERSATPPPNNARTLWFSIEVEDTGPGIPEHLQERVFEPFVQADLGLNRKYGGTGLGLSICSQLAGLMGGSISLVSTLGEGSTFNVQLPLKFVKERAPSTSSSDVIGSRPASMVSQSELERHTPKGSIDATTAVGSPGKSNGGAGYSIDTQPRLVGLRQPFFTSSPTPPPNDDNAKTQLAAIDRAAKEKVGDKKIRVLVAEDNLVNQEVVLRMLKLEDVYDVVVAKDGQEAYDIVKQSMEEGKFFNLIFMDIQMPNLDGLQSTRLIREMGYSAPIVALTAFAEESNVKECYESGMDHFLSKPIRRPALKQVLKKFATIPEEIEDSSITRKSTSEKGQASKQQNKNTSTSTFSPASNSDADLIKMNGTPSPPSPLTST

Target ORF information:

RefSeq Version	XM_001553044.2
Organism	Botrytis cinerea B05.10
Definition	Botrytis cinerea B05.10 Bchhk5 (Bchhk5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001553044.2

ORF Insert Sequence:

ATGAGGATTG GTATCAGGCT GCAACTTGGC CTGGTTGTCC TGGTGGCTGC TCTTGTTCCA 
TTGATTGTGC TCGCTCTCGC TACCTGGTTC AACAATTATA GTTTTACAGT CGACGTCAAA 
GAAAATGCAC TTAGCCTTAC GGCATCACTC AAAGCCGCTT CAGTGGCTTC CGATCTACTC 
CTAATCCAGG CCACATGCTC AACTATCGTC ACGAGGATCC TTATCCAGGA GGCCATCCGC 
GACTTTTATA GAGGAGCACC CGAGTGGAAC GAAGCAAGAG AAGATGTACA AAGCGCTTTG 
GCTTCTGGTG GTCTATCTGC ATTGCTTCAA GTAATCATAT TTTCGCGGAA TGAGACGGGC 
TCGGTCAATG GTATATTAAA TGTCACAGCC AATGCATCCG ATATACAACT GCCCTATAAT 
AACTCGCTCA ACCAGCCCGC TTTCCTAGGC GATGCTGATC AGGGATTTCC AGCGGCTTTG 
TATCCGAATA TCTCTTATAT CCCAACCACG GATCCCGACC CAAATGCTCC AGGAACGAAT 
TTTACACAGG TGTACGCATT TGCCGATTAC CCACTGAATA AAAGTGCTGC TCTTCTACTG 
GGGCCGTTAC AAATTAATAG CACTTATGCT TTAGTATCAC TGACCTTACC GATTGTGGAG 
AATTCGAATC CCGATAACAT TCTAGGTTAT ATGACTGTGG TAGCAGCTGC GACTTCGCTG 
ATTGACGTTG TTTATTCTCG GGAGGGCTTG GATAAAACAG GAATAGTGTT GGTAGTGGGA 
ACCAATCGAC GAGAGAACCA GTTCTCGTAC ATGTCGAGAC CGGCTACAGC AACTTACGAA 
CCCGACCTAC ATAATTTGAC AAAAGCAAGT GTCAAATACA TCTTCCCGCC ATACCCTCTC 
GAAGGGCAAA CTGATAGACA TTCCGTCTAC AATGCAAATT TAACTGAATA TGGGAGCTCA 
AATTTTAGCG AAGGCAATTA TCCGGCTATT CTACATGGAT TCGGACGACA GAATCCGAAA 
GTCAATAATG CCAGTAGTTA TATTACAACC ACAAACGAGC AAGGAGTAAA GGTGTCGGTA 
GGCTGGGCAA GACCACAAAG TGCACTGGTT GATTGGCTTT TGATCGTAGA ACAAGCTCAT 
TCCGAAGCTT GGGAACCCAT TATCAAGCTT AGGAATATCT TACTTGCATG TGTTTTTGGT 
ACATTTGGGT TGATCCTTAT TGTAGTTATA CCGATGGCTC ATTTCAGTGT TCGACCAATC 
AGAAGGCTAA GAGATGCCAC GAAACAATCC ATTCAGCCAC CGGGCTACAC TCCGCGTCAA 
AGTATAGAGG ATGAATTATA TGGCGAGGAA GAAGTCGGCG AGGGTAGTTC AAGTGGGTAC 
AAGAAAACGG GATTTTTGGT CAGGCTGAGA AAATTAACAC AGTCGGGGCG TCGTGTGTCG 
AGAGCAGAAA AATCAGAGAT CGACAGACGG AGAGATTTTA AAATTCCTGG AAAGGTGCCT 
GATCGAAAAC ACTGGATTGA GGACGAACTC ACGGATTTAA CATCGAAATT TAATGAAATG 
AGCGACGAAT TACTGCTGCA ATACACGAGT CTCGAATCCA AAGTTGCTGA ACGTACGGAA 
CAACTTGAAC ATTCCAAGAA GGCAGCGGAG GCAGCAAACG AGAGCAAAAC ATTATTCATT 
GCAAACATAT CGCACGAATT GAAAACTCCA TTGAACGGAA TTCTGGGAAT GTGTGCCGTA 
TGCATGGGTG AGGATGATTT GCCAAGCATC AAACGTTCAT TGAAGACGGT TTACAAATCT 
GGTGACCTTT TGTTGAATTT ATTGAATGAT CTTTTGACGT TCAGTAAGAA CCAGATTGGC 
CAGCAATTGC ATCTAGAAGA GAAAGAGTTT CGGCTGTCTG ATGTAAAGGA GCAGATCAGG 
AACATATTCC GAAAGCAAGT GGACGAAGGC GGTATTACTT TTGGAGTCCA CTTCCTGGGC 
ACTGAAAGCA CAGATGGAGC TCCACTAGAG AAACCGAGCG GAAAGCCTCT ACCAGCCCTA 
GGACCTAATG GTATTGGTAG ATTGAAAGAT ATGTGTTTGT GGGGTGATCA GCACAGAATT 
CTCCAAATCA TAATCAATCT AGTCAGCAAC AGTCTCAAAT TCACACCACC AGGAGGAAAG 
GTAGAAGTCA GAATTAGATG TATGGGCGAA TTGGAACAAC CATTGGATGG TGTCAATAGC 
CGAAATTCCA TGGGTTCGAA GCAGAGCTCT CACAGGAGAA ATGTTAGAAC CCGTACTGGC 
TCAGGATCGA ATGCTTCAGG GCATACTTCG ACGCATGCAT CGGGGCAAAT TTCAAGGACA 
GCTTCAAATA CCAAGCAAGC TGGGACTGCA CTAGTAATCA ATCCTATGGA TCCAAGGGCT 
CGTGTCCAAT TAAACGAGCG ATCGGCAACT CCGCCACCAA ATAATGCTAG GACTCTATGG 
TTTTCAATAG AGGTTGAGGA TACTGGACCC GGTATTCCCG AGCATCTACA GGAGCGAGTT 
TTCGAGCCTT TCGTACAGGC TGATCTTGGG TTGAATAGGA AATATGGAGG AACCGGATTG 
GGATTAAGTA TTTGCTCCCA GTTAGCAGGC CTTATGGGAG GTTCTATCTC TCTCGTTAGT 
ACACTTGGAG AAGGTTCCAC GTTCAATGTT CAGTTGCCAT TGAAATTCGT CAAGGAGCGA 
GCACCAAGTA CATCTAGTTC CGATGTTATT GGTTCTCGGC CCGCAAGCAT GGTTTCGCAA 
TCAGAACTCG AACGTCATAC TCCAAAGGGT TCAATTGATG CAACGACTGC TGTAGGATCC 
CCTGGGAAAA GCAATGGTGG TGCAGGCTAT TCAATAGATA CTCAACCTCG GTTGGTGGGA 
CTTCGACAGC CATTCTTCAC ATCATCCCCT ACACCTCCAC CCAATGACGA TAACGCAAAA 
ACTCAACTCG CTGCAATCGA TCGCGCTGCC AAGGAGAAAG TAGGGGATAA GAAAATTCGA 
GTTCTGGTTG CCGAAGATAA TCTTGTTAAT CAAGAGGTGG TTCTTCGCAT GCTTAAACTT 
GAAGACGTGT ATGACGTGGT GGTCGCCAAG GATGGGCAGG AGGCATACGA TATAGTAAAG 
CAAAGCATGG AAGAGGGCAA ATTCTTCAAC TTGATCTTTA TGGATATTCA AATGCCAAAT 
CTGGATGGGC TACAAAGTAC GAGATTAATC AGAGAAATGG GTTATTCTGC GCCCATTGTA 
GCACTAACGG CTTTTGCCGA AGAGAGTAAT GTCAAAGAAT GCTATGAGTC CGGAATGGAT 
CATTTCCTAA GTAAACCTAT TCGACGACCA GCTCTCAAGC AAGTTCTTAA GAAATTCGCC 
ACTATCCCAG AGGAGATAGA AGACTCCTCC ATAACTCGAA AAAGCACATC AGAAAAAGGG 
CAAGCATCGA AACAGCAAAA CAAAAATACA TCTACTTCAA CATTCAGTCC GGCATCAAAT 
TCTGATGCTG ACCTCATTAA AATGAATGGG ACCCCTTCAC CTCCATCACC TTTAACCTCT 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bchhk5 ( XM_024697058.1 ) cDNA ORF clone, Botrytis cinerea B05.10 -> XP_024552872.1 Botrytis cinerea B05.10 Bchhk5 (Bchhk5), mRNA.

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CloneID	OBc23475
Clone ID Related Accession (Same CDS sequence)	XM_024697058.1
Accession Version	XM_024697058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523808000000
Organism	Botrytis cinerea B05.10
Product	Bchhk5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_037323).

ATGAGGATTG GTATCAGGCT GCAACTTGGC CTGGTTGTCC TGGTGGCTGC TCTTGTTCCA 
TTGATTGTGC TCGCTCTCGC TACCTGGTTC AACAATTATA GTTTTACAGT CGACGTCAAA 
GAAAATGCAC TTAGCCTTAC GGCATCACTC AAAGCCGCTT CAGTGGCTTC CGATCTACTC 
CTAATCCAGG CCACATGCTC AACTATCGTC ACGAGGATCC TTATCCAGGA GGCCATCCGC 
GACTTTTATA GAGGAGCACC CGAGTGGAAC GAAGCAAGAG AAGATGTACA AAGCGCTTTG 
GCTTCTGGTG GTCTATCTGC ATTGCTTCAA GTAATCATAT TTTCGCGGAA TGAGACGGGC 
TCGGTCAATG GTATATTAAA TGTCACAGCC AATGCATCCG GTGTTGCAGA TATACAACTG 
CCCTATAATA ACTCGCTCAA CCAGCCCGCT TTCCTAGGCG ATGCTGATCA GGGATTTCCA 
GCGGCTTTGT ATCCGAATAT CTCTTATATC CCAACCACGG ATCCCGACCC AAATGCTCCA 
GGAACGAATT TTACACAGGT GTACGCATTT GCCGATTACC CACTGAATAA AAGTGCTGCT 
CTTCTACTGG GGCCGTTACA AATTAATAGC ACTTATGCTT TAGTATCACT GACCTTACCG 
ATTGTGGAGA ATTCGAATCC CGATAACATT CTAGGTTATA TGACTGTGGT AGCAGCTGCG 
ACTTCGCTGA TTGACGTTGT TTATTCTCGG GAGGGCTTGG ATAAAACAGG AATAGTGTTG 
GTAGTGGGAA CCAATCGACG AGAGAACCAG TTCTCGTACA TGTCGAGACC GGCTACAGCA 
ACTTACGAAC CCGACCTACA TAATTTGACA AAAGCAAGTG TCAAATACAT CTTCCCGCCA 
TACCCTCTCG AAGGGCAAAC TGATAGACAT TCCGTCTACA ATGCAAATTT AACTGAATAT 
GGGAGCTCAA ATTTTAGCGA AGGCAATTAT CCGGCTATTC TACATGGATT CGGACGACAG 
AATCCGAAAG TCAATAATGC CAGTAGTTAT ATTACAACCA CAAACGAGCA AGGAGTAAAG 
GTGTCGGTAG GCTGGGCAAG ACCACAAAGT GCACTGGTTG ATTGGCTTTT GATCGTAGAA 
CAAGCTCATT CCGAAGCTTG GGAACCCATT ATCAAGCTTA GGAATATCTT ACTTGCATGT 
GTTTTTGGTA CATTTGGGTT GATCCTTATT GTAGTTATAC CGATGGCTCA TTTCAGTGTT 
CGACCAATCA GAAGGCTAAG AGATGCCACG AAACAATCCA TTCAGCCACC GGGCTACACT 
CCGCGTCAAA GTATAGAGGA TGAATTATAT GGCGAGGAAG AAGTCGGCGA GGGTAGTTCA 
AGTGGGTACA AGAAAACGGG ATTTTTGGTC AGGCTGAGAA AATTAACACA GTCGGGGCGT 
CGTGTGTCGA GAGCAGAAAA ATCAGAGATC GACAGACGGA GAGATTTTAA AATTCCTGGA 
AAGGTGCCTG ATCGAAAACA CTGGATTGAG GACGAACTCA CGGATTTAAC ATCGAAATTT 
AATGAAATGA GCGACGAATT ACTGCTGCAA TACACGAGTC TCGAATCCAA AGTTGCTGAA 
CGTACGGAAC AACTTGAACA TTCCAAGAAG GCAGCGGAGG CAGCAAACGA GAGCAAAACA 
TTATTCATTG CAAACATATC GCACGAATTG AAAACTCCAT TGAACGGAAT TCTGGGAATG 
TGTGCCGTAT GCATGGGTGA GGATGATTTG CCAAGCATCA AACGTTCATT GAAGACGGTT 
TACAAATCTG GTGACCTTTT GTTGAATTTA TTGAATGATC TTTTGACGTT CAGTAAGAAC 
CAGATTGGCC AGCAATTGCA TCTAGAAGAG AAAGAGTTTC GGCTGTCTGA TGTAAAGGAG 
CAGATCAGGA ACATATTCCG AAAGCAAGTG GACGAAGGCG GTATTACTTT TGGAGTCCAC 
TTCCTGGGCA CTGAAAGCAC AGATGGAGCT CCACTAGAGA AACCGAGCGG AAAGCCTCTA 
CCAGCCCTAG GACCTAATGG TATTGGTAGA TTGAAAGATA TGTGTTTGTG GGGTGATCAG 
CACAGAATTC TCCAAATCAT AATCAATCTA GTCAGCAACA GTCTCAAATT CACACCACCA 
GGAGGAAAGG TAGAAGTCAG AATTAGATGT ATGGGCGAAT TGGAACAACC ATTGGATGGT 
GTCAATAGCC GAAATTCCAT GGGTTCGAAG CAGAGCTCTC ACAGGAGAAA TGTTAGAACC 
CGTACTGGCT CAGGATCGAA TGCTTCAGGG CATACTTCGA CGCATGCATC GGGGCAAATT 
TCAAGGACAG CTTCAAATAC CAAGCAAGCT GGGACTGCAC TAGTAATCAA TCCTATGGAT 
CCAAGGGCTC GTGTCCAATT AAACGAGCGA TCGGCAACTC CGCCACCAAA TAATGCTAGG 
ACTCTATGGT TTTCAATAGA GGTTGAGGAT ACTGGACCCG GTATTCCCGA GCATCTACAG 
GAGCGAGTTT TCGAGCCTTT CGTACAGGCT GATCTTGGGT TGAATAGGAA ATATGGAGGA 
ACCGGATTGG GATTAAGTAT TTGCTCCCAG TTAGCAGGCC TTATGGGAGG TTCTATCTCT 
CTCGTTAGTA CACTTGGAGA AGGTTCCACG TTCAATGTTC AGTTGCCATT GAAATTCGTC 
AAGGAGCGAG CACCAAGTAC ATCTAGTTCC GATGTTATTG GTTCTCGGCC CGCAAGCATG 
GTTTCGCAAT CAGAACTCGA ACGTCATACT CCAAAGGGTT CAATTGATGC AACGACTGCT 
GTAGGATCCC CTGGGAAAAG CAATGGTGGT GCAGGCTATT CAATAGATAC TCAACCTCGG 
TTGGTGGGAC TTCGACAGCC ATTCTTCACA TCATCCCCTA CACCTCCACC CAATGACGAT 
AACGCAAAAA CTCAACTCGC TGCAATCGAT CGCGCTGCCA AGGAGAAAGT AGGGGATAAG 
AAAATTCGAG TTCTGGTTGC CGAAGATAAT CTTGTTAATC AAGAGGTGGT TCTTCGCATG 
CTTAAACTTG AAGACGTGTA TGACGTGGTG GTCGCCAAGG ATGGGCAGGA GGCATACGAT 
ATAGTAAAGC AAAGCATGGA AGAGGGCAAA TTCTTCAACT TGATCTTTAT GGATATTCAA 
ATGCCAAATC TGGATGGGCT ACAAAGTACG AGATTAATCA GAGAAATGGG TTATTCTGCG 
CCCATTGTAG CACTAACGGC TTTTGCCGAA GAGAGTAATG TCAAAGAATG CTATGAGTCC 
GGAATGGATC ATTTCCTAAG TAAACCTATT CGACGACCAG CTCTCAAGCA AGTTCTTAAG 
AAATTCGCCA CTATCCCAGA GGAGATAGAA GACTCCTCCA TAACTCGAAA AAGCACATCA 
GAAAAAGGGC AAGCATCGAA ACAGCAAAAC AAAAATACAT CTACTTCAAC ATTCAGTCCG 
GCATCAAATT CTGATGCTGA CCTCATTAAA ATGAATGGGA CCCCTTCACC TCCATCACCT 
TTAACCTCTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024552872.1
CDS	785..4339
Translation	MRIGIRLQLGLVVLVAALVPLIVLALATWFNNYSFTVDVKENALSLTASLKAASVASDLLLIQATCSTIVTRILIQEAIRDFYRGAPEWNEAREDVQSALASGGLSALLQVIIFSRNETGSVNGILNVTANASGVADIQLPYNNSLNQPAFLGDADQGFPAALYPNISYIPTTDPDPNAPGTNFTQVYAFADYPLNKSAALLLGPLQINSTYALVSLTLPIVENSNPDNILGYMTVVAAATSLIDVVYSREGLDKTGIVLVVGTNRRENQFSYMSRPATATYEPDLHNLTKASVKYIFPPYPLEGQTDRHSVYNANLTEYGSSNFSEGNYPAILHGFGRQNPKVNNASSYITTTNEQGVKVSVGWARPQSALVDWLLIVEQAHSEAWEPIIKLRNILLACVFGTFGLILIVVIPMAHFSVRPIRRLRDATKQSIQPPGYTPRQSIEDELYGEEEVGEGSSSGYKKTGFLVRLRKLTQSGRRVSRAEKSEIDRRRDFKIPGKVPDRKHWIEDELTDLTSKFNEMSDELLLQYTSLESKVAERTEQLEHSKKAAEAANESKTLFIANISHELKTPLNGILGMCAVCMGEDDLPSIKRSLKTVYKSGDLLLNLLNDLLTFSKNQIGQQLHLEEKEFRLSDVKEQIRNIFRKQVDEGGITFGVHFLGTESTDGAPLEKPSGKPLPALGPNGIGRLKDMCLWGDQHRILQIIINLVSNSLKFTPPGGKVEVRIRCMGELEQPLDGVNSRNSMGSKQSSHRRNVRTRTGSGSNASGHTSTHASGQISRTASNTKQAGTALVINPMDPRARVQLNERSATPPPNNARTLWFSIEVEDTGPGIPEHLQERVFEPFVQADLGLNRKYGGTGLGLSICSQLAGLMGGSISLVSTLGEGSTFNVQLPLKFVKERAPSTSSSDVIGSRPASMVSQSELERHTPKGSIDATTAVGSPGKSNGGAGYSIDTQPRLVGLRQPFFTSSPTPPPNDDNAKTQLAAIDRAAKEKVGDKKIRVLVAEDNLVNQEVVLRMLKLEDVYDVVVAKDGQEAYDIVKQSMEEGKFFNLIFMDIQMPNLDGLQSTRLIREMGYSAPIVALTAFAEESNVKECYESGMDHFLSKPIRRPALKQVLKKFATIPEEIEDSSITRKSTSEKGQASKQQNKNTSTSTFSPASNSDADLIKMNGTPSPPSPLTST

Target ORF information:

RefSeq Version	XM_024697058.1
Organism	Botrytis cinerea B05.10
Definition	Botrytis cinerea B05.10 Bchhk5 (Bchhk5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024697058.1

ORF Insert Sequence:

ATGAGGATTG GTATCAGGCT GCAACTTGGC CTGGTTGTCC TGGTGGCTGC TCTTGTTCCA 
TTGATTGTGC TCGCTCTCGC TACCTGGTTC AACAATTATA GTTTTACAGT CGACGTCAAA 
GAAAATGCAC TTAGCCTTAC GGCATCACTC AAAGCCGCTT CAGTGGCTTC CGATCTACTC 
CTAATCCAGG CCACATGCTC AACTATCGTC ACGAGGATCC TTATCCAGGA GGCCATCCGC 
GACTTTTATA GAGGAGCACC CGAGTGGAAC GAAGCAAGAG AAGATGTACA AAGCGCTTTG 
GCTTCTGGTG GTCTATCTGC ATTGCTTCAA GTAATCATAT TTTCGCGGAA TGAGACGGGC 
TCGGTCAATG GTATATTAAA TGTCACAGCC AATGCATCCG GTGTTGCAGA TATACAACTG 
CCCTATAATA ACTCGCTCAA CCAGCCCGCT TTCCTAGGCG ATGCTGATCA GGGATTTCCA 
GCGGCTTTGT ATCCGAATAT CTCTTATATC CCAACCACGG ATCCCGACCC AAATGCTCCA 
GGAACGAATT TTACACAGGT GTACGCATTT GCCGATTACC CACTGAATAA AAGTGCTGCT 
CTTCTACTGG GGCCGTTACA AATTAATAGC ACTTATGCTT TAGTATCACT GACCTTACCG 
ATTGTGGAGA ATTCGAATCC CGATAACATT CTAGGTTATA TGACTGTGGT AGCAGCTGCG 
ACTTCGCTGA TTGACGTTGT TTATTCTCGG GAGGGCTTGG ATAAAACAGG AATAGTGTTG 
GTAGTGGGAA CCAATCGACG AGAGAACCAG TTCTCGTACA TGTCGAGACC GGCTACAGCA 
ACTTACGAAC CCGACCTACA TAATTTGACA AAAGCAAGTG TCAAATACAT CTTCCCGCCA 
TACCCTCTCG AAGGGCAAAC TGATAGACAT TCCGTCTACA ATGCAAATTT AACTGAATAT 
GGGAGCTCAA ATTTTAGCGA AGGCAATTAT CCGGCTATTC TACATGGATT CGGACGACAG 
AATCCGAAAG TCAATAATGC CAGTAGTTAT ATTACAACCA CAAACGAGCA AGGAGTAAAG 
GTGTCGGTAG GCTGGGCAAG ACCACAAAGT GCACTGGTTG ATTGGCTTTT GATCGTAGAA 
CAAGCTCATT CCGAAGCTTG GGAACCCATT ATCAAGCTTA GGAATATCTT ACTTGCATGT 
GTTTTTGGTA CATTTGGGTT GATCCTTATT GTAGTTATAC CGATGGCTCA TTTCAGTGTT 
CGACCAATCA GAAGGCTAAG AGATGCCACG AAACAATCCA TTCAGCCACC GGGCTACACT 
CCGCGTCAAA GTATAGAGGA TGAATTATAT GGCGAGGAAG AAGTCGGCGA GGGTAGTTCA 
AGTGGGTACA AGAAAACGGG ATTTTTGGTC AGGCTGAGAA AATTAACACA GTCGGGGCGT 
CGTGTGTCGA GAGCAGAAAA ATCAGAGATC GACAGACGGA GAGATTTTAA AATTCCTGGA 
AAGGTGCCTG ATCGAAAACA CTGGATTGAG GACGAACTCA CGGATTTAAC ATCGAAATTT 
AATGAAATGA GCGACGAATT ACTGCTGCAA TACACGAGTC TCGAATCCAA AGTTGCTGAA 
CGTACGGAAC AACTTGAACA TTCCAAGAAG GCAGCGGAGG CAGCAAACGA GAGCAAAACA 
TTATTCATTG CAAACATATC GCACGAATTG AAAACTCCAT TGAACGGAAT TCTGGGAATG 
TGTGCCGTAT GCATGGGTGA GGATGATTTG CCAAGCATCA AACGTTCATT GAAGACGGTT 
TACAAATCTG GTGACCTTTT GTTGAATTTA TTGAATGATC TTTTGACGTT CAGTAAGAAC 
CAGATTGGCC AGCAATTGCA TCTAGAAGAG AAAGAGTTTC GGCTGTCTGA TGTAAAGGAG 
CAGATCAGGA ACATATTCCG AAAGCAAGTG GACGAAGGCG GTATTACTTT TGGAGTCCAC 
TTCCTGGGCA CTGAAAGCAC AGATGGAGCT CCACTAGAGA AACCGAGCGG AAAGCCTCTA 
CCAGCCCTAG GACCTAATGG TATTGGTAGA TTGAAAGATA TGTGTTTGTG GGGTGATCAG 
CACAGAATTC TCCAAATCAT AATCAATCTA GTCAGCAACA GTCTCAAATT CACACCACCA 
GGAGGAAAGG TAGAAGTCAG AATTAGATGT ATGGGCGAAT TGGAACAACC ATTGGATGGT 
GTCAATAGCC GAAATTCCAT GGGTTCGAAG CAGAGCTCTC ACAGGAGAAA TGTTAGAACC 
CGTACTGGCT CAGGATCGAA TGCTTCAGGG CATACTTCGA CGCATGCATC GGGGCAAATT 
TCAAGGACAG CTTCAAATAC CAAGCAAGCT GGGACTGCAC TAGTAATCAA TCCTATGGAT 
CCAAGGGCTC GTGTCCAATT AAACGAGCGA TCGGCAACTC CGCCACCAAA TAATGCTAGG 
ACTCTATGGT TTTCAATAGA GGTTGAGGAT ACTGGACCCG GTATTCCCGA GCATCTACAG 
GAGCGAGTTT TCGAGCCTTT CGTACAGGCT GATCTTGGGT TGAATAGGAA ATATGGAGGA 
ACCGGATTGG GATTAAGTAT TTGCTCCCAG TTAGCAGGCC TTATGGGAGG TTCTATCTCT 
CTCGTTAGTA CACTTGGAGA AGGTTCCACG TTCAATGTTC AGTTGCCATT GAAATTCGTC 
AAGGAGCGAG CACCAAGTAC ATCTAGTTCC GATGTTATTG GTTCTCGGCC CGCAAGCATG 
GTTTCGCAAT CAGAACTCGA ACGTCATACT CCAAAGGGTT CAATTGATGC AACGACTGCT 
GTAGGATCCC CTGGGAAAAG CAATGGTGGT GCAGGCTATT CAATAGATAC TCAACCTCGG 
TTGGTGGGAC TTCGACAGCC ATTCTTCACA TCATCCCCTA CACCTCCACC CAATGACGAT 
AACGCAAAAA CTCAACTCGC TGCAATCGAT CGCGCTGCCA AGGAGAAAGT AGGGGATAAG 
AAAATTCGAG TTCTGGTTGC CGAAGATAAT CTTGTTAATC AAGAGGTGGT TCTTCGCATG 
CTTAAACTTG AAGACGTGTA TGACGTGGTG GTCGCCAAGG ATGGGCAGGA GGCATACGAT 
ATAGTAAAGC AAAGCATGGA AGAGGGCAAA TTCTTCAACT TGATCTTTAT GGATATTCAA 
ATGCCAAATC TGGATGGGCT ACAAAGTACG AGATTAATCA GAGAAATGGG TTATTCTGCG 
CCCATTGTAG CACTAACGGC TTTTGCCGAA GAGAGTAATG TCAAAGAATG CTATGAGTCC 
GGAATGGATC ATTTCCTAAG TAAACCTATT CGACGACCAG CTCTCAAGCA AGTTCTTAAG 
AAATTCGCCA CTATCCCAGA GGAGATAGAA GACTCCTCCA TAACTCGAAA AAGCACATCA 
GAAAAAGGGC AAGCATCGAA ACAGCAAAAC AAAAATACAT CTACTTCAAC ATTCAGTCCG 
GCATCAAATT CTGATGCTGA CCTCATTAAA ATGAATGGGA CCCCTTCACC TCCATCACCT 
TTAACCTCTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A gapless genome sequence of the fungus Botrytis cinerea.
Molecular plant pathology18(1)75-89(2017 01)
Van Kan JA,Stassen JH,Mosbach A,Van Der Lee TA,Faino L,Farmer AD,Papasotiriou DG,Zhou S,Seidl MF,Cottam E,Edel D,Hahn M,Schwartz DC,Dietrich RA,Widdison S,Scalliet G

Genome update of Botrytis cinerea strains B05.10 and T4.
Eukaryotic cell11(11)1413-4(2012 Nov)
Staats M,van Kan JA

Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
PLoS genetics7(8)e1002230(2011 Aug)
Amselem J,Cuomo CA,van Kan JA,Viaud M,Benito EP,Couloux A,Coutinho PM,de Vries RP,Dyer PS,Fillinger S,Fournier E,Gout L,Hahn M,Kohn L,Lapalu N,Plummer KM,Pradier JM,Quévillon E,Sharon A,Simon A,ten Have A,Tudzynski B,Tudzynski P,Wincker P,Andrew M,Anthouard V,Beever RE,Beffa R,Benoit I,Bouzid O,Brault B,Chen Z,Choquer M,Collémare J,Cotton P,Danchin EG,Da Silva C,Gautier A,Giraud C,Giraud T,Gonzalez C,Grossetete S,Güldener U,Henrissat B,Howlett BJ,Kodira C,Kretschmer M,Lappartient A,Leroch M,Levis C,Mauceli E,Neuvéglise C,Oeser B,Pearson M,Poulain J,Poussereau N,Quesneville H,Rascle C,Schumacher J,Ségurens B,Sexton A,Silva E,Sirven C,Soanes DM,Talbot NJ,Templeton M,Yandava C,Yarden O,Zeng Q,Rollins JA,Lebrun MH,Dickman M

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Bchhk5 cDNA ORF clone, Botrytis cinerea B05.10

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