KHK cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	KHK
Entrez Gene ID	614868
Full Name	ketohexokinase
General protein information	Preferred Names ketohexokinase Names ketohexokinase ketohexokinase (fructokinase)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024999150.1	XP_024854918.1	ketohexokinase isoform X3
XM_024999149.1	XP_024854917.1	ketohexokinase isoform X2
XM_024999148.1	XP_024854916.1	ketohexokinase isoform X1
XM_024999151.1	XP_024854919.1	ketohexokinase isoform X4
NM_001076351.1	NP_001069819.1	ketohexokinase
XM_024999152.1	XP_024854920.1	ketohexokinase isoform X5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KHK	XP_003949817.1
Mus musculus (house mouse)	Khk	NP_032465.2
Rattus norvegicus (Norway rat)	Khk	NP_114061.1
Xenopus tropicalis (tropical clawed frog)	khk	XP_002943517.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002483	XP_563692.3
Danio rerio (zebrafish)	khk	NP_001002636.1
Canis lupus familiaris (dog)	KHK	NP_001182084.1
Homo sapiens (human)	KHK	NP_000212.1
Macaca mulatta (Rhesus monkey)	KHK	XP_001089839.1
Bos taurus (cattle)	KHK	NP_001069819.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KHK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KHK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa37878	NM_001076351.1 Latest version!	Bos taurus ketohexokinase (KHK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa220623	XM_024999148.1 Latest version!	Bos taurus ketohexokinase (KHK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa220624	XM_024999149.1 Latest version!	Bos taurus ketohexokinase (KHK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa220625	XM_024999150.1 Latest version!	Bos taurus ketohexokinase (KHK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa220626	XM_024999151.1 Latest version!	Bos taurus ketohexokinase (KHK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa220627	XM_024999152.1 Latest version!	Bos taurus ketohexokinase (KHK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KHK ( NM_001076351.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069819.1 Bos taurus ketohexokinase (KHK), mRNA.

Price & Availability↑

CloneID	OBa37878
Clone ID Related Accession (Same CDS sequence)	NM_001076351.1
Accession Version	NM_001076351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	ketohexokinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC122772.1. On or before Oct 4, 2006 this sequence version replaced XM_880143.1, XM_867407.1. ##Evidence-Data-START## Transcript exon combination :: BC122772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAGA AACAGATTCT GTGCGTGGGG CTAGTGGTGC TGGACATCAT CAATGTGATG 
GACAAATACC CAGAGGAGGA CACGGACAGC AGGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAT TTTGTCCTGG ACGACCTTCG CCGCTATTCT 
GTGGACCTCC GCTACATGGT CTTTCAGACC ACGGGCTCCG TCCCCATCTC CACAGTCATC 
ATCAGCGAGG CCAGCGGTAG CCGCACCATC CTACATGCCT ACAGCTTCCT GGTGGCTGAC 
TTCAGGCAGC GGGGCGTGGA CGTGTCTCAG GTGGCCTGGC AGAGCAGAGG GGAGACCCCG 
TGCTCTTGTT GCATCATCAA CAACTCCAAT GGCTCCCGTA CCATTGTACT CTACGACACG 
AACCTGCCAG ATGTGTCCGC TAAAGACTTT GAGAAGGTTG AGCTGACCCG GTTCAAGTGG 
ATCCACATTG AGGGCCGGAA CGCATCAGAG CAGGTGAAGA TGCTACAGCG GATAGAACAG 
CACAATGCCA GGCAGCCTCC AGAAAGCAAG ATCCAGGTGT CTGTGGAGGT GGAGAAGCCA 
CGTGAGGAGC TGTACCAGCT GTTTGGTTAT GGAGATGTGG TGTTTGTCAG CAAAGATGTG 
GCCAAGCACT TCGGGTTCCG GTCAGCAAAG GAGGCCCTGA GGGGCCTGTA TAGCCGCGTG 
AGGAAGGGGG CCACGCTGGT CTGTGCCTGG GCGGAGGAGG GCGCCGACGC GCTGGGGCCT 
GATGGACGGC TGCTCCACTC CGATGCTTTC CCACCGCCCC GCGTGGTGGA CACTCTGGGG 
GCCGGAGACA CCTTCAACGC GGCGGTCATC TTCAGCCTGT CCCAGGGGAA GAGCATGCAG 
GAGGCACTGA GGTTCGGCTG CCAGGTGGCC GGCAAGAAGT GCGGTCTGCA GGGTTTTGAT 
GGCATTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069819.1
CDS	59..1090
Translation	MEEKQILCVGLVVLDIINVMDKYPEEDTDSRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSLARGHVADFVLDDLRRYSVDLRYMVFQTTGSVPISTVIISEASGSRTILHAYSFLVADFRQRGVDVSQVAWQSRGETPCSCCIINNSNGSRTIVLYDTNLPDVSAKDFEKVELTRFKWIHIEGRNASEQVKMLQRIEQHNARQPPESKIQVSVEVEKPREELYQLFGYGDVVFVSKDVAKHFGFRSAKEALRGLYSRVRKGATLVCAWAEEGADALGPDGRLLHSDAFPPPRVVDTLGAGDTFNAAVIFSLSQGKSMQEALRFGCQVAGKKCGLQGFDGIV

Target ORF information:

RefSeq Version	NM_001076351.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ketohexokinase (KHK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076351.1

ORF Insert Sequence:

ATGGAAGAGA AACAGATTCT GTGCGTGGGG CTAGTGGTGC TGGACATCAT CAATGTGATG 
GACAAATACC CAGAGGAGGA CACGGACAGC AGGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAT TTTGTCCTGG ACGACCTTCG CCGCTATTCT 
GTGGACCTCC GCTACATGGT CTTTCAGACC ACGGGCTCCG TCCCCATCTC CACAGTCATC 
ATCAGCGAGG CCAGCGGTAG CCGCACCATC CTACATGCCT ACAGCTTCCT GGTGGCTGAC 
TTCAGGCAGC GGGGCGTGGA CGTGTCTCAG GTGGCCTGGC AGAGCAGAGG GGAGACCCCG 
TGCTCTTGTT GCATCATCAA CAACTCCAAT GGCTCCCGTA CCATTGTACT CTACGACACG 
AACCTGCCAG ATGTGTCCGC TAAAGACTTT GAGAAGGTTG AGCTGACCCG GTTCAAGTGG 
ATCCACATTG AGGGCCGGAA CGCATCAGAG CAGGTGAAGA TGCTACAGCG GATAGAACAG 
CACAATGCCA GGCAGCCTCC AGAAAGCAAG ATCCAGGTGT CTGTGGAGGT GGAGAAGCCA 
CGTGAGGAGC TGTACCAGCT GTTTGGTTAT GGAGATGTGG TGTTTGTCAG CAAAGATGTG 
GCCAAGCACT TCGGGTTCCG GTCAGCAAAG GAGGCCCTGA GGGGCCTGTA TAGCCGCGTG 
AGGAAGGGGG CCACGCTGGT CTGTGCCTGG GCGGAGGAGG GCGCCGACGC GCTGGGGCCT 
GATGGACGGC TGCTCCACTC CGATGCTTTC CCACCGCCCC GCGTGGTGGA CACTCTGGGG 
GCCGGAGACA CCTTCAACGC GGCGGTCATC TTCAGCCTGT CCCAGGGGAA GAGCATGCAG 
GAGGCACTGA GGTTCGGCTG CCAGGTGGCC GGCAAGAAGT GCGGTCTGCA GGGTTTTGAT 
GGCATTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KHK ( XM_024999148.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854916.1 Bos taurus ketohexokinase (KHK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa220623
Clone ID Related Accession (Same CDS sequence)	XM_024999148.1
Accession Version	XM_024999148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ketohexokinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCCT CACAGCAGCC CATGGGAAAC ATGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAT TTTGTCCTGG ACGACCTTCG CCGCTATTCT 
GTGGACCTCC GCTACATGGT CTTTCAGACC ACGGGCTCCG TCCCCATCTC CACAGTCATC 
ATCAGCGAGG CCAGCGGTAG CCGCACCATC CTACATGCCT ACAGCTTCCT GGTGGCTGAC 
TTCAGGCAGC GGGGCGTGGA CGTGTCTCAG GTGGCCTGGC AGAGCAGAGG GGAGACCCCG 
TGCTCTTGTT GCATCATCAA CAACTCCAAT GGCTCCCGTA CCATTGTACT CTACGACACG 
AACCTGCCAG ATGTGTCCGC TAAAGACTTT GAGAAGGTTG AGCTGACCCG GTTCAAGTGG 
ATCCACATTG AGGGCCGGAA CGCATCAGAG CAGGTGAAGA TGCTACAGCG GATAGAACAG 
CACAATGCCA GGCAGCCTCC AGAAAGCAAG ATCCAGGTGT CTGTGGAGGT GGAGAAGCCA 
CGTGAGGAGC TGTACCAGCT GTTTGGTTAT GGAGATGTGG TGTTTGTCAG CAAAGATGTG 
GCCAAGCACT TCGGGTTCCG GTCAGCAAAG GAGGCCCTGA GGGGCCTGTA TAGCCGCGTG 
AGGAAGGGGG CCACGCTGGT CTGTGCCTGG GCGGAGGAGG GCGCCGACGC GCTGGGGCCT 
GATGGACGGC TGCTCCACTC CGATGCTTTC CCACCGCCCC GCGTGGTGGA CACTCTGGGG 
GCCGGAGACA CCTTCAACGC GGCGGTCATC TTCAGCCTGT CCCAGGGGAA GAGCATGCAG 
GAGGCACTGA GGTTCGGCTG CCAGGTGGCC GGCAAGAAGT GCGGTCTGCA GGGTTTTGAT 
GGCATTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854916.1
CDS	105..1076
Translation	MLSSQQPMGNMCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSLARGHVADFVLDDLRRYSVDLRYMVFQTTGSVPISTVIISEASGSRTILHAYSFLVADFRQRGVDVSQVAWQSRGETPCSCCIINNSNGSRTIVLYDTNLPDVSAKDFEKVELTRFKWIHIEGRNASEQVKMLQRIEQHNARQPPESKIQVSVEVEKPREELYQLFGYGDVVFVSKDVAKHFGFRSAKEALRGLYSRVRKGATLVCAWAEEGADALGPDGRLLHSDAFPPPRVVDTLGAGDTFNAAVIFSLSQGKSMQEALRFGCQVAGKKCGLQGFDGIV

Target ORF information:

RefSeq Version	XM_024999148.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ketohexokinase (KHK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999148.1

ORF Insert Sequence:

ATGCTTTCCT CACAGCAGCC CATGGGAAAC ATGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAT TTTGTCCTGG ACGACCTTCG CCGCTATTCT 
GTGGACCTCC GCTACATGGT CTTTCAGACC ACGGGCTCCG TCCCCATCTC CACAGTCATC 
ATCAGCGAGG CCAGCGGTAG CCGCACCATC CTACATGCCT ACAGCTTCCT GGTGGCTGAC 
TTCAGGCAGC GGGGCGTGGA CGTGTCTCAG GTGGCCTGGC AGAGCAGAGG GGAGACCCCG 
TGCTCTTGTT GCATCATCAA CAACTCCAAT GGCTCCCGTA CCATTGTACT CTACGACACG 
AACCTGCCAG ATGTGTCCGC TAAAGACTTT GAGAAGGTTG AGCTGACCCG GTTCAAGTGG 
ATCCACATTG AGGGCCGGAA CGCATCAGAG CAGGTGAAGA TGCTACAGCG GATAGAACAG 
CACAATGCCA GGCAGCCTCC AGAAAGCAAG ATCCAGGTGT CTGTGGAGGT GGAGAAGCCA 
CGTGAGGAGC TGTACCAGCT GTTTGGTTAT GGAGATGTGG TGTTTGTCAG CAAAGATGTG 
GCCAAGCACT TCGGGTTCCG GTCAGCAAAG GAGGCCCTGA GGGGCCTGTA TAGCCGCGTG 
AGGAAGGGGG CCACGCTGGT CTGTGCCTGG GCGGAGGAGG GCGCCGACGC GCTGGGGCCT 
GATGGACGGC TGCTCCACTC CGATGCTTTC CCACCGCCCC GCGTGGTGGA CACTCTGGGG 
GCCGGAGACA CCTTCAACGC GGCGGTCATC TTCAGCCTGT CCCAGGGGAA GAGCATGCAG 
GAGGCACTGA GGTTCGGCTG CCAGGTGGCC GGCAAGAAGT GCGGTCTGCA GGGTTTTGAT 
GGCATTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KHK ( XM_024999149.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854917.1 Bos taurus ketohexokinase (KHK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa220624
Clone ID Related Accession (Same CDS sequence)	XM_024999149.1
Accession Version	XM_024999149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ketohexokinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA AACAGATTCT GTGCGTGGGG CTAGTGGTGC TGGACATCAT CAATGTGATG 
GACAAATACC CAGAGGAGGA CACGGACAGC AGGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAT TTTGTCCTGG ACGACCTTCG CCGCTATTCT 
GTGGACCTCC GCTACATGGT CTTTCAGACC ACGGGCTCCG TCCCCATCTC CACAGTCATC 
ATCAGCGAGG CCAGCGGTAG CCGCACCATC CTACATGCCT ACAGGAACCT GCCAGATGTG 
TCCGCTAAAG ACTTTGAGAA GGTTGAGCTG ACCCGGTTCA AGTGGATCCA CATTGAGGGC 
CGGAACGCAT CAGAGCAGGT GAAGATGCTA CAGCGGATAG AACAGCACAA TGCCAGGCAG 
CCTCCAGAAA GCAAGATCCA GGTGTCTGTG GAGGTGGAGA AGCCACGTGA GGAGCTGTAC 
CAGCTGTTTG GTTATGGAGA TGTGGTGTTT GTCAGCAAAG ATGTGGCCAA GCACTTCGGG 
TTCCGGTCAG CAAAGGAGGC CCTGAGGGGC CTGTATAGCC GCGTGAGGAA GGGGGCCACG 
CTGGTCTGTG CCTGGGCGGA GGAGGGCGCC GACGCGCTGG GGCCTGATGG ACGGCTGCTC 
CACTCCGATG CTTTCCCACC GCCCCGCGTG GTGGACACTC TGGGGGCCGG AGACACCTTC 
AACGCGGCGG TCATCTTCAG CCTGTCCCAG GGGAAGAGCA TGCAGGAGGC ACTGAGGTTC 
GGCTGCCAGG TGGCCGGCAA GAAGTGCGGT CTGCAGGGTT TTGATGGCAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854917.1
CDS	549..1445
Translation	MEEKQILCVGLVVLDIINVMDKYPEEDTDSRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSLARGHVADFVLDDLRRYSVDLRYMVFQTTGSVPISTVIISEASGSRTILHAYRNLPDVSAKDFEKVELTRFKWIHIEGRNASEQVKMLQRIEQHNARQPPESKIQVSVEVEKPREELYQLFGYGDVVFVSKDVAKHFGFRSAKEALRGLYSRVRKGATLVCAWAEEGADALGPDGRLLHSDAFPPPRVVDTLGAGDTFNAAVIFSLSQGKSMQEALRFGCQVAGKKCGLQGFDGIV

Target ORF information:

RefSeq Version	XM_024999149.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ketohexokinase (KHK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999149.1

ORF Insert Sequence:

ATGGAAGAGA AACAGATTCT GTGCGTGGGG CTAGTGGTGC TGGACATCAT CAATGTGATG 
GACAAATACC CAGAGGAGGA CACGGACAGC AGGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAT TTTGTCCTGG ACGACCTTCG CCGCTATTCT 
GTGGACCTCC GCTACATGGT CTTTCAGACC ACGGGCTCCG TCCCCATCTC CACAGTCATC 
ATCAGCGAGG CCAGCGGTAG CCGCACCATC CTACATGCCT ACAGGAACCT GCCAGATGTG 
TCCGCTAAAG ACTTTGAGAA GGTTGAGCTG ACCCGGTTCA AGTGGATCCA CATTGAGGGC 
CGGAACGCAT CAGAGCAGGT GAAGATGCTA CAGCGGATAG AACAGCACAA TGCCAGGCAG 
CCTCCAGAAA GCAAGATCCA GGTGTCTGTG GAGGTGGAGA AGCCACGTGA GGAGCTGTAC 
CAGCTGTTTG GTTATGGAGA TGTGGTGTTT GTCAGCAAAG ATGTGGCCAA GCACTTCGGG 
TTCCGGTCAG CAAAGGAGGC CCTGAGGGGC CTGTATAGCC GCGTGAGGAA GGGGGCCACG 
CTGGTCTGTG CCTGGGCGGA GGAGGGCGCC GACGCGCTGG GGCCTGATGG ACGGCTGCTC 
CACTCCGATG CTTTCCCACC GCCCCGCGTG GTGGACACTC TGGGGGCCGG AGACACCTTC 
AACGCGGCGG TCATCTTCAG CCTGTCCCAG GGGAAGAGCA TGCAGGAGGC ACTGAGGTTC 
GGCTGCCAGG TGGCCGGCAA GAAGTGCGGT CTGCAGGGTT TTGATGGCAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KHK ( XM_024999150.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854918.1 Bos taurus ketohexokinase (KHK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa220625
Clone ID Related Accession (Same CDS sequence)	XM_024999150.1
Accession Version	XM_024999150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ketohexokinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA AACAGATTCT GTGCGTGGGG CTAGTGGTGC TGGACATCAT CAATGTGATG 
GACAAATACC CAGAGGAGGA CACGGACAGC AGGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAC TTCCTGGTGG CTGACTTCAG GCAGCGGGGC 
GTGGACGTGT CTCAGGTGGC CTGGCAGAGC AGAGGGGAGA CCCCGTGCTC TTGTTGCATC 
ATCAACAACT CCAATGGCTC CCGTACCATT GTACTCTACG ACACGAACCT GCCAGATGTG 
TCCGCTAAAG ACTTTGAGAA GGTTGAGCTG ACCCGGTTCA AGTGGATCCA CATTGAGGGC 
CGGAACGCAT CAGAGCAGGT GAAGATGCTA CAGCGGATAG AACAGCACAA TGCCAGGCAG 
CCTCCAGAAA GCAAGATCCA GGTGTCTGTG GAGGTGGAGA AGCCACGTGA GGAGCTGTAC 
CAGCTGTTTG GTTATGGAGA TGTGGTGTTT GTCAGCAAAG ATGTGGCCAA GCACTTCGGG 
TTCCGGTCAG CAAAGGAGGC CCTGAGGGGC CTGTATAGCC GCGTGAGGAA GGGGGCCACG 
CTGGTCTGTG CCTGGGCGGA GGAGGGCGCC GACGCGCTGG GGCCTGATGG ACGGCTGCTC 
CACTCCGATG CTTTCCCACC GCCCCGCGTG GTGGACACTC TGGGGGCCGG AGACACCTTC 
AACGCGGCGG TCATCTTCAG CCTGTCCCAG GGGAAGAGCA TGCAGGAGGC ACTGAGGTTC 
GGCTGCCAGG TGGCCGGCAA GAAGTGCGGT CTGCAGGGTT TTGATGGCAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854918.1
CDS	529..1425
Translation	MEEKQILCVGLVVLDIINVMDKYPEEDTDSRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSLARGHVADFLVADFRQRGVDVSQVAWQSRGETPCSCCIINNSNGSRTIVLYDTNLPDVSAKDFEKVELTRFKWIHIEGRNASEQVKMLQRIEQHNARQPPESKIQVSVEVEKPREELYQLFGYGDVVFVSKDVAKHFGFRSAKEALRGLYSRVRKGATLVCAWAEEGADALGPDGRLLHSDAFPPPRVVDTLGAGDTFNAAVIFSLSQGKSMQEALRFGCQVAGKKCGLQGFDGIV

Target ORF information:

RefSeq Version	XM_024999150.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ketohexokinase (KHK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999150.1

ORF Insert Sequence:

ATGGAAGAGA AACAGATTCT GTGCGTGGGG CTAGTGGTGC TGGACATCAT CAATGTGATG 
GACAAATACC CAGAGGAGGA CACGGACAGC AGGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAC TTCCTGGTGG CTGACTTCAG GCAGCGGGGC 
GTGGACGTGT CTCAGGTGGC CTGGCAGAGC AGAGGGGAGA CCCCGTGCTC TTGTTGCATC 
ATCAACAACT CCAATGGCTC CCGTACCATT GTACTCTACG ACACGAACCT GCCAGATGTG 
TCCGCTAAAG ACTTTGAGAA GGTTGAGCTG ACCCGGTTCA AGTGGATCCA CATTGAGGGC 
CGGAACGCAT CAGAGCAGGT GAAGATGCTA CAGCGGATAG AACAGCACAA TGCCAGGCAG 
CCTCCAGAAA GCAAGATCCA GGTGTCTGTG GAGGTGGAGA AGCCACGTGA GGAGCTGTAC 
CAGCTGTTTG GTTATGGAGA TGTGGTGTTT GTCAGCAAAG ATGTGGCCAA GCACTTCGGG 
TTCCGGTCAG CAAAGGAGGC CCTGAGGGGC CTGTATAGCC GCGTGAGGAA GGGGGCCACG 
CTGGTCTGTG CCTGGGCGGA GGAGGGCGCC GACGCGCTGG GGCCTGATGG ACGGCTGCTC 
CACTCCGATG CTTTCCCACC GCCCCGCGTG GTGGACACTC TGGGGGCCGG AGACACCTTC 
AACGCGGCGG TCATCTTCAG CCTGTCCCAG GGGAAGAGCA TGCAGGAGGC ACTGAGGTTC 
GGCTGCCAGG TGGCCGGCAA GAAGTGCGGT CTGCAGGGTT TTGATGGCAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KHK ( XM_024999151.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854919.1 Bos taurus ketohexokinase (KHK), transcript variant X4, mRNA.

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CloneID	OBa220626
Clone ID Related Accession (Same CDS sequence)	XM_024999151.1
Accession Version	XM_024999151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ketohexokinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCCT CACAGCAGCC CATGGGAAAC ATGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAC TTCCTGGTGG CTGACTTCAG GCAGCGGGGC 
GTGGACGTGT CTCAGGTGGC CTGGCAGAGC AGAGGGGAGA CCCCGTGCTC TTGTTGCATC 
ATCAACAACT CCAATGGCTC CCGTACCATT GTACTCTACG ACACGAACCT GCCAGATGTG 
TCCGCTAAAG ACTTTGAGAA GGTTGAGCTG ACCCGGTTCA AGTGGATCCA CATTGAGGGC 
CGGAACGCAT CAGAGCAGGT GAAGATGCTA CAGCGGATAG AACAGCACAA TGCCAGGCAG 
CCTCCAGAAA GCAAGATCCA GGTGTCTGTG GAGGTGGAGA AGCCACGTGA GGAGCTGTAC 
CAGCTGTTTG GTTATGGAGA TGTGGTGTTT GTCAGCAAAG ATGTGGCCAA GCACTTCGGG 
TTCCGGTCAG CAAAGGAGGC CCTGAGGGGC CTGTATAGCC GCGTGAGGAA GGGGGCCACG 
CTGGTCTGTG CCTGGGCGGA GGAGGGCGCC GACGCGCTGG GGCCTGATGG ACGGCTGCTC 
CACTCCGATG CTTTCCCACC GCCCCGCGTG GTGGACACTC TGGGGGCCGG AGACACCTTC 
AACGCGGCGG TCATCTTCAG CCTGTCCCAG GGGAAGAGCA TGCAGGAGGC ACTGAGGTTC 
GGCTGCCAGG TGGCCGGCAA GAAGTGCGGT CTGCAGGGTT TTGATGGCAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854919.1
CDS	105..941
Translation	MLSSQQPMGNMCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSLARGHVADFLVADFRQRGVDVSQVAWQSRGETPCSCCIINNSNGSRTIVLYDTNLPDVSAKDFEKVELTRFKWIHIEGRNASEQVKMLQRIEQHNARQPPESKIQVSVEVEKPREELYQLFGYGDVVFVSKDVAKHFGFRSAKEALRGLYSRVRKGATLVCAWAEEGADALGPDGRLLHSDAFPPPRVVDTLGAGDTFNAAVIFSLSQGKSMQEALRFGCQVAGKKCGLQGFDGIV

Target ORF information:

RefSeq Version	XM_024999151.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ketohexokinase (KHK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999151.1

ORF Insert Sequence:

ATGCTTTCCT CACAGCAGCC CATGGGAAAC ATGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAC TTCCTGGTGG CTGACTTCAG GCAGCGGGGC 
GTGGACGTGT CTCAGGTGGC CTGGCAGAGC AGAGGGGAGA CCCCGTGCTC TTGTTGCATC 
ATCAACAACT CCAATGGCTC CCGTACCATT GTACTCTACG ACACGAACCT GCCAGATGTG 
TCCGCTAAAG ACTTTGAGAA GGTTGAGCTG ACCCGGTTCA AGTGGATCCA CATTGAGGGC 
CGGAACGCAT CAGAGCAGGT GAAGATGCTA CAGCGGATAG AACAGCACAA TGCCAGGCAG 
CCTCCAGAAA GCAAGATCCA GGTGTCTGTG GAGGTGGAGA AGCCACGTGA GGAGCTGTAC 
CAGCTGTTTG GTTATGGAGA TGTGGTGTTT GTCAGCAAAG ATGTGGCCAA GCACTTCGGG 
TTCCGGTCAG CAAAGGAGGC CCTGAGGGGC CTGTATAGCC GCGTGAGGAA GGGGGCCACG 
CTGGTCTGTG CCTGGGCGGA GGAGGGCGCC GACGCGCTGG GGCCTGATGG ACGGCTGCTC 
CACTCCGATG CTTTCCCACC GCCCCGCGTG GTGGACACTC TGGGGGCCGG AGACACCTTC 
AACGCGGCGG TCATCTTCAG CCTGTCCCAG GGGAAGAGCA TGCAGGAGGC ACTGAGGTTC 
GGCTGCCAGG TGGCCGGCAA GAAGTGCGGT CTGCAGGGTT TTGATGGCAT TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KHK ( XM_024999152.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854920.1 Bos taurus ketohexokinase (KHK), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa220627
Clone ID Related Accession (Same CDS sequence)	XM_024999152.1
Accession Version	XM_024999152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ketohexokinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA AACAGATTCT GTGCGTGGGG CTAGTGGTGC TGGACATCAT CAATGTGATG 
GACAAATACC CAGAGGAGGA CACGGACAGC AGGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAG AACCTGCCAG ATGTGTCCGC TAAAGACTTT 
GAGAAGGTTG AGCTGACCCG GTTCAAGTGG ATCCACATTG AGGGCCGGAA CGCATCAGAG 
CAGGTGAAGA TGCTACAGCG GATAGAACAG CACAATGCCA GGCAGCCTCC AGAAAGCAAG 
ATCCAGGTGT CTGTGGAGGT GGAGAAGCCA CGTGAGGAGC TGTACCAGCT GTTTGGTTAT 
GGAGATGTGG TGTTTGTCAG CAAAGATGTG GCCAAGCACT TCGGGTTCCG GTCAGCAAAG 
GAGGCCCTGA GGGGCCTGTA TAGCCGCGTG AGGAAGGGGG CCACGCTGGT CTGTGCCTGG 
GCGGAGGAGG GCGCCGACGC GCTGGGGCCT GATGGACGGC TGCTCCACTC CGATGCTTTC 
CCACCGCCCC GCGTGGTGGA CACTCTGGGG GCCGGAGACA CCTTCAACGC GGCGGTCATC 
TTCAGCCTGT CCCAGGGGAA GAGCATGCAG GAGGCACTGA GGTTCGGCTG CCAGGTGGCC 
GGCAAGAAGT GCGGTCTGCA GGGTTTTGAT GGCATTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854920.1
CDS	529..1290
Translation	MEEKQILCVGLVVLDIINVMDKYPEEDTDSRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSLARGHVAENLPDVSAKDFEKVELTRFKWIHIEGRNASEQVKMLQRIEQHNARQPPESKIQVSVEVEKPREELYQLFGYGDVVFVSKDVAKHFGFRSAKEALRGLYSRVRKGATLVCAWAEEGADALGPDGRLLHSDAFPPPRVVDTLGAGDTFNAAVIFSLSQGKSMQEALRFGCQVAGKKCGLQGFDGIV

Target ORF information:

RefSeq Version	XM_024999152.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ketohexokinase (KHK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999152.1

ORF Insert Sequence:

ATGGAAGAGA AACAGATTCT GTGCGTGGGG CTAGTGGTGC TGGACATCAT CAATGTGATG 
GACAAATACC CAGAGGAGGA CACGGACAGC AGGTGCTTGT CCCAGAGATG GCAACGCGGA 
GGCAATGCAT CCAACTCCTG CACTGTCCTC TCCCTGCTTG GAGCCCCCTG CGCCTTCATG 
GGCTCGCTGG CCCGGGGCCA CGTTGCTGAG AACCTGCCAG ATGTGTCCGC TAAAGACTTT 
GAGAAGGTTG AGCTGACCCG GTTCAAGTGG ATCCACATTG AGGGCCGGAA CGCATCAGAG 
CAGGTGAAGA TGCTACAGCG GATAGAACAG CACAATGCCA GGCAGCCTCC AGAAAGCAAG 
ATCCAGGTGT CTGTGGAGGT GGAGAAGCCA CGTGAGGAGC TGTACCAGCT GTTTGGTTAT 
GGAGATGTGG TGTTTGTCAG CAAAGATGTG GCCAAGCACT TCGGGTTCCG GTCAGCAAAG 
GAGGCCCTGA GGGGCCTGTA TAGCCGCGTG AGGAAGGGGG CCACGCTGGT CTGTGCCTGG 
GCGGAGGAGG GCGCCGACGC GCTGGGGCCT GATGGACGGC TGCTCCACTC CGATGCTTTC 
CCACCGCCCC GCGTGGTGGA CACTCTGGGG GCCGGAGACA CCTTCAACGC GGCGGTCATC 
TTCAGCCTGT CCCAGGGGAA GAGCATGCAG GAGGCACTGA GGTTCGGCTG CCAGGTGGCC 
GGCAAGAAGT GCGGTCTGCA GGGTTTTGAT GGCATTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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KHK cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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