BRWD1 cDNA ORF clone, Bos taurus(Cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRWD1
Entrez Gene ID	540661
Full Name	bromodomain and WD repeat domain containing 1
General protein information	Preferred Names bromodomain and WD repeat domain containing 1 Names bromodomain and WD repeat-containing protein 1
Gene Type	protein-coding
Organism	Bos taurus(Cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103174.2	NP_001096644.1	bromodomain and WD repeat-containing protein 1 isoform 1
XM_024991350.1	XP_024847118.1	bromodomain and WD repeat-containing protein 1 isoform X1
NM_001354349.1	NP_001341278.1	bromodomain and WD repeat-containing protein 1 isoform 2
NM_001103174.1	NP_001096644.1	bromodomain and WD repeat-containing protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BRWD1	XP_001170924.1
Gallus gallus (chicken)	BRWD1	XP_416730.4
Canis lupus familiaris (dog)	BRWD1	XP_544889.4
Bos taurus (cattle)	BRWD1	NP_001096644.1
Homo sapiens (human)	BRWD1	NP_061836.2
Mus musculus (house mouse)	Brwd1	NP_660107.2
Rattus norvegicus (Norway rat)	Brwd1	XP_006248248.1
Danio rerio (zebrafish)	brwd1	XP_001920441.4
Xenopus tropicalis (tropical clawed frog)	brwd1	XP_002938777.2
Macaca mulatta (Rhesus monkey)	BRWD1	XP_001108760.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRWD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRWD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa216249	NM_001354349.1 Latest version!	Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OBa40619	NM_001103174.2 Latest version!	Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa218889	XM_024991350.1 Latest version!	Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OBa40619	NM_001103174.1	Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRWD1 ( NM_001354349.1 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001341278.1 Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OBa216249
Clone ID Related Accession (Same CDS sequence)	NM_001354349.1
Accession Version	NM_001354349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	bromodomain and WD repeat-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH138768.1 and BC142396.1. ##Evidence-Data-START## Transcript exon combination :: BC142396.1, DV906656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN05861764, SAMN05861786 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CCTCGCCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA ACTGTACTTC 
CTCATCGCCC GGTACCTATC AGCCGGCCCA TGTCGGAGAG CCGCCCAGGT GTTGGTGCAG 
GAGCTGGAGC AGTATCAGTT GCTGCCGAAG AGGTTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAATTGGT CTTGTCCAAT AAGCATGTGG CTCCTGACCA TCTGTTGCAA 
ATCTGCCAAC GCATCGGTCC CATGCTGGAT AAAGAGATCC CACCCAGCAT TTCAAGAGTC 
ACTTCTTTAC TTGGGGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGG TACCTTAATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341278.1
CDS	124..486
Translation	MAEPSPARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKGTLI

Target ORF information:

RefSeq Version	NM_001354349.1
Organism	Bos taurus(cattle)
Definition	Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354349.1

ORF Insert Sequence:

ATGGCGGAGC CCTCGCCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA ACTGTACTTC 
CTCATCGCCC GGTACCTATC AGCCGGCCCA TGTCGGAGAG CCGCCCAGGT GTTGGTGCAG 
GAGCTGGAGC AGTATCAGTT GCTGCCGAAG AGGTTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAATTGGT CTTGTCCAAT AAGCATGTGG CTCCTGACCA TCTGTTGCAA 
ATCTGCCAAC GCATCGGTCC CATGCTGGAT AAAGAGATCC CACCCAGCAT TTCAAGAGTC 
ACTTCTTTAC TTGGGGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGG TACCTTAATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( NM_001103174.2 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001096644.1 Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa40619
Clone ID Related Accession (Same CDS sequence)	NM_001103174.2 , NM_001103174.1
Accession Version	NM_001103174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548086400000
Organism	Bos taurus(cattle)
Product	bromodomain and WD repeat-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH138768.1, EH137476.1, NKLS02000001.1, DY195028.1, DV848547.1 and EV626973.1. On Aug 10, 2017 this sequence version replaced NM_001103174.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CCTCGCCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA ACTGTACTTC 
CTCATCGCCC GGTACCTATC AGCCGGCCCA TGTCGGAGAG CCGCCCAGGT GTTGGTGCAG 
GAGCTGGAGC AGTATCAGTT GCTGCCGAAG AGGTTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAATTGGT CTTGTCCAAT AAGCATGTGG CTCCTGACCA TCTGTTGCAA 
ATCTGCCAAC GCATCGGTCC CATGCTGGAT AAAGAGATCC CACCCAGCAT TTCAAGAGTC 
ACTTCTTTAC TTGGGGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCG 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATCCATC GAGGAAAACA GCTCACAGGG 
TGTTCCACTT TTAGCACAGC ATTTCCAGGA GCTATGTATC AGCACATAAA AATGCACAGG 
AGGATTCTCG GACATCTGTC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA TTGTTTGGTG AAGATTTGGT CCACACACAA TGGCCGCCTA 
TTGTCCACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCAGGGAG CTGTGATAAA ATAATTAGAG TGTGGTGCCT GAGAACCTGT 
GCTCCAGTTG CTGTGCTCCA AGGACACACG GGGTCAATTA CATCTTTACA GTTTAGCCCC 
ATGGCCAAAG GCTCGCAAAG ATACATGGTC TCCACTGGTG CTGATGGGAC TGTTTGTTTT 
TGGCAGTGGG ATTTAGAATC CCTGAAATTC AGTCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGACCAG GTGTTCAAAT GCTGTGTTCT TCATTTAGTG TTGGTGGTAT GTTTCTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCTGAAAAA 
ATCGCAGAAC TTGAAAGTCA CACTGATAAA GTTGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCT TAAGTGGTAG CAGAGATGGA ACAGCACGAA TTTGGAGATT TGAACAGTTA 
GAATGGAGAA GCATCTTACT AGATATGGCC ACTAGAATCT CAGGAGATTT ATCTTTGGAA 
GAGGAAAGGT TTATGAAACC CAAAGTAACA ATGATAGCTT GGAATCAGAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAGCTG 
CTTCATAACC TGTTGGGACA TGCTGATGAA GTATTTGTTT TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAC GGCAGCATAT TCATATGGGA TATCACAAAA 
GGCACCAAGA TGAAACATTA TTTCAATATG ATTGAAGGAC AAGGACATGG AGCTGTCTTT 
GATTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGTCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAGCCA TATGAAAAGA TTCCTGACCA GATGTTCTTC 
CATACTGACT ATCGACCGCT GATTAGGGAT TCTAATAATT ACGTGTTAGA CGAGCAGACT 
CAGCAGGCCC CTCACCTTAT GCCTCCACCG TTCTTGGTAG ATGTAGATGG AAACCCTCAT 
CCAACTAAGT ATCAGCGATT AGTGCCTGGC CGAGAAAATT CTGCAGATGA ACATTTGGTT 
CCACAACTAG GCTATGTGGC AACAAGTGAT GGAGAGGTAA TAGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGGTGA ACGAAGCCCA GAGCGCAGTG TCCTGGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGGTC AGGAGGCTGT TCCAAGTGGA 
CTTTCAAATG GGGAAGAAAC ACCCCGAAGA GGTTTTAGAA GACTAAGCTT AGACATCCAG 
TCTCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTGG AAGGTGTCCG TCAGATGCAC 
CAGAATGCTC CGCGCAGTCA GATGGCTACG GAGCGTGACC TGCAGGCTTG GAAGCGAAGA 
GTGGTTGTCC CAGAGGTACC ACTAGGCATA TTTAGGAAGT TGGAAGACTT CAGAATAGAG 
AAAGGTGAAC AAGAAAGAAA TCTTTATATA ATTGGAAGAA AGAGGAAGAC TCTCCAGTTC 
TCACAAAAGT CTGATTCGGT GACTTTTATG TCACAGTCTA GACAGAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCTT GGACGGCTTG AGTTGTCTTC TGGAAATGAA 
TCTTCAAGCT CCATAAGACA TGAGACTTCT TGTGATCAAA GTGAAGGTTC TTGTTCTTCT 
GAAGAAGAAG AATGGAAAAG TGATAGAAGG AGTGAAAGTG ACAGTGAAAG TTCAAGTGAC 
TCTTCCTCTA GGTATTCTGA TTGGACAGCT GACGCAGGCA TCCACCTGCA GCCTCCTTTA 
CGAACATCAT CTCGTCGACG AATTACTCGA TTTTGCAGCA GTTCAGAGGA TGAAATGTCT 
GCTGAGAATT TATCTCCTCC AAAAAGGAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAA 
AAAGAGAATT TGCGGAGGAT GACTCAGGCA GAGCTTGCAG ACGTGGAACA TTTATATGAA 
TTTCATCCTC CAGTTTGGAT AACTGACACC ACACTTCGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTCAGA 
AGAAATAACA TTTATGAACT GAACCCTAAC AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTCAAAAT AGTTGGAATA CGATATGAGG TTGGACCCCC GACACTTTGT 
TGCCTCAAAC TAGCATTTAT AGATCCTGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATACC ATGACATGCC AGATGTTATT GACTTTCTTG TCTTGCGTCA GTTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGGTTCC GTTCCATCAT TGATGATGCT 
TGGTGGTTTG GAACGGTGCT ACGTCAAGAG CCGTATCAGC CACAGTACCC TGATAGTCAT 
TTCCAGTGTT ACATTGTGAG GTGGGACAAT ACTGAAATTG AAAAACTTAG CCCATGGGAT 
ATGGAACCAA TACCTGACAA TGTTGATCCA CCTGAAGAAT TAGGCGCTAG TATTTCTGTC 
ACTTCAGATG AGCTAGAGAA ATTGCTCTAC AAACCCCAAG ATGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA ACGAATTATT AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATTGCAGCAG CTTTTGCAGG TCCGGTTGAT CTGTGTACAT ATCCGAAGTA CTGTACTGTA 
GTGGCTTACC CAACTGATCT GTACACCATT CGAATGAGAC TTGTGAATCG ATTTTACAGG 
AGGCTATCTG CGTTAGTTTG GGAAGTCAGA TACATAGAAC ACAATGCCAG AACATTTAAT 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAGAAGATAA CAGACCAGCT TTTAAAATTT 
ATCAAGAATC AAGACTGTAT AAATATCTCA GAACTTTCTA ACACTTCTGA AAATGATGAG 
CAAAATGCCG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGAAGGAGA 
GTTCATGATT GGAAAAAAAG GAGCATCAAA ACAACCAATT ATGTTGAAAA CAACTGGAAG 
AAACAGTGTA AGGAACTAGT GAACTTAATT TTTCAATGTG AAGATTCTGA ACCATTTAGA 
CAACCTGTTG ATTTGGTTGA ATATCCAGAT TACCGAGACA TTATAGATAC TCCAATGGAT 
TTTGGAACAG TAAGGGAAAC TTTAGAAGCG GGAAACTATG ACAGCCCTTT GGAATTTTGC 
AAAGACATCC GTTTGATATT TAGCAATGCA AAAGCATATA CACCAAACAA AAGATCAAAG 
ATTTACAGTA TGACATTGAG ATTGTCTGCC TTATTTGAAG AAAAAATGAA GAAAATCTCC 
TCTGATTTTA AAATTGGTCA AAAATTCAGT GAAAAACTTC GAAGAAGCCA GAGGTTTAAG 
AGACGACAAA ATTGTAACCG TGTCAATCAG GCTAACAAAA GTATCAGAAA TACCAAGCAG 
AAGCGTTTAA AATCTCAGAC AAAAGTAATT TCTGAGTTCG TAGGTTCTCC TATCCAGCCT 
ACCTCAAGTA GGGCAACTTA CTCCGCAAGC CACAAGACAA CTGCTAGTGT CTCTTCAGGT 
GTTACTTCTG GTGACTCTTC AGATTCAGCA GCATCATCAG AAAGAATGAG AAGAAATAGA 
CCTGTCACTC TGACAAATGG TTCTACATTA TCTGAAAGTG AAATGGAAGA TTCTTTAGGT 
ACCTCTTCAT CATCTTCAGC TTCAAATAGT TCTGAGGAAA GCAAGGAGAG TCCAAGAGCT 
CGAGACTCCT CCTCCCGTAG TGGGCTAGCC AGGAGCAGCA ACCTCAGGGT GACCAGGACC 
AGAGCTGCAC AGAGAAAAAC TGGTCCAGTT TCTTTAGAAA ATGGATGTGG CAGAAAAGCC 
ACTAGGAAGA GAGTCTATTT AAGTGACTCA GATAACAATT CTTTGGCGAC TGGTGAAAGT 
GTAAAAGTGA GAGCTGGAAA TAACCGGAAA GTGCTACGAA AATGTGCTGC TGTGGCTGCC 
AATAAAATCA AGTTAATGAG TGATGTAGAG GAGAATTCTA CCTCTGAAAG TGTCTGTTCT 
GGCCGTAAGC TGCCCCACCG CAATGCTTCT GCTGTAGCTA GAAAAAAATT ATTACATAAT 
TCTGAGGATG ACCAGAGCTT GAAGTCAGAA CTTGAAGAAG AGGAGCTAAA AGATCAACTA 
TCACCATTGT CCAACTCTCT TGCTGCCCAG AATACTGAAA ATGGAGATTC AGAGTCAGAA 
AGTGATTTAC GGGTAGCCCG GAAAAATTGG CATGCTAATG GTTACAAGTC CCAAATTCCA 
GCAACTTCTA AAACAAAATT CCTTAAAATA GCATCTTCTG AGGAAGACTC TAAAAGTCAT 
GATTCAGATA ATGGACATAG CAGAACTGCT GGCCCATCAA CATCTTTGCT GAAACTCAAG 
GCAGAAAGCA TTTCAGAGGA AGAGGAGAAA GCAAATTCTG AAACAAGAAA ATACAGCAGT 
AGGAAATACA GCACAGGTCG CAAGAATGCT CCTTTCCTTA AAAAAGCAAA GCTCTTCAGT 
GATTCAGAGG ACTCTGAGTC TGACAAAGAA GATGGACAAG ACAGATGTCA TAAGATGGAA 
ATGAACCCGA GTTCAGGGAA TGTGGAATGT GAACCTGCTG CCAGATCCCA GTGTTTTTCA 
GATCATGTAT CTGAAACTGA TTTGGATTCA GATGATGACA AGAAAGCAAA AGAAAAACCA 
AATAGTTTTA TAAAAGATTC TGCATCACAA AATCATGGAC AGAGCAGGAA AGCTGCCAGG 
AAAAGGGTCT GTTCCAGTGA CTCAGACAGC AATTCGAAGG TGGTTAAGAA AGCATCCAGA 
GCCAGACCAG GCCTTCTGAG AGTGCCTCGG AGGTGTGCCA CCACTGCTGC CAGTCGGATC 
AAGCTGATGA GTGATGAGGA AGATGGCCGT GTAGACCACG TGTGCACCAG GCGCAAAAAT 
GCAAGGAAAA AGCCCCTCCA TATTGCATGT GCCCCAGCTC AGAAGATAGA CAGCAATTCT 
GAGGGGGATG TAAACTGTGA AGTGCCAAAC GAACAGAGTG GCTGTGAAGG GCAGCCACTT 
GATGCCGACT CAGAAGGTAG TGCAAAAAGT ATCAGTCAGT CTTTAAATGA AGACTCAGAC 
TCTGAAGGTG TGTTGAATTC AGGACACAAG AACAGGCATA CCAGGAGTCT CAAAACCAGT 
GTTGCATCTT CTAAAACAAA GAATGCCTTG ATTGTGTCTT TGGAGGAAGA TTCAAAAAGC 
CATATTCCAC GGGGTGAGAT AGGTAGAAAA TGTTCTTCTG AGTCAACTTT GGTGCACAAA 
GCTACTGCAG AGAATAACTT TGAAGAGGAA CTGAACTATG GGCTACGTAG GTGGAACGGC 
AGGAGGCTCC GGACCTATGG GAAGGCTCCT TTCAGTAAGA CAAAAGTGGT GCGGGATTCG 
CAGGAAGCTG CCGAGACAGA AGTAAAAAGG AAGAGACTGC AAACTGAATT GGAAAATGTG 
AAAGCTTCCG AAACAACAGG GAGTTCAAAG TGTGGACCAG ACACTAGCCC CAAATCTGAC 
TCAGATTTGG GATCTGCCAC TGAATCAGAT GCTGACTGTA CAGATGATTC AAAAACCAAA 
AAGAGGAAAA CGAAAGGGAA AGCAAAAGTA GTTAGAAAAG AATCTGTTCC TAGAGACAGA 
GAACCCAATA CAAAAATGAG AACATGTGTG CATAATCAGA AGGATGCAGT GCCAATGCCT 
AGTGAGACTC TGAAAGCAAA AATGGTACCT GAGAAAGTTC CCCGCAGATG TGCTACTGTT 
GCTGCAAATA AAATAAAGAT AATGAGTAAT CTAAAGGAAA CCATTTCAGG ACCAGAAAAT 
GTGTGGATTA GGAAGAGCAG CAGAAAACTG CCCCATCGAA ATGCTTCTGC TGCGGCTAAG 
AAAAAATTAC TCAATGTTTA TAAAGAGGAT GATACAACCA TAAATTCTGA AAGTGAAAAG 
GAGCTAGAAG ATATTAATAG AAAAATGCCC CTTTTAAGGC GTTTCAGATC CTGGAAAGAA 
AAAGCACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096644.1
CDS	124..7095
Translation	MAEPSPARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGAMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSLEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLLGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLVPQLGYVATSDGEVIEQIISLQTNDNGERSPERSVLDGMIRQLQQQQDQRMGAGQEAVPSGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQMATERDLQAWKRRVVVPEVPLGIFRKLEDFRIEKGEQERNLYIIGRKRKTLQFSQKSDSVTFMSQSRQRTCRRKYPNYGRRNLGRLELSSGNESSSSIRHETSCDQSEGSCSSEEEEWKSDRRSESDSESSSDSSSRYSDWTADAGIHLQPPLRTSSRRRITRFCSSSEDEMSAENLSPPKRRRKRKKENKPKKENLRRMTQAELADVEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLRQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTSDELEKLLYKPQDGEWGQKSRDEECERIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQDCINISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDWKKRSIKTTNYVENNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLEAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFSEKLRRSQRFKRRQNCNRVNQANKSIRNTKQKRLKSQTKVISEFVGSPIQPTSSRATYSASHKTTASVSSGVTSGDSSDSAASSERMRRNRPVTLTNGSTLSESEMEDSLGTSSSSSASNSSEESKESPRARDSSSRSGLARSSNLRVTRTRAAQRKTGPVSLENGCGRKATRKRVYLSDSDNNSLATGESVKVRAGNNRKVLRKCAAVAANKIKLMSDVEENSTSESVCSGRKLPHRNASAVARKKLLHNSEDDQSLKSELEEEELKDQLSPLSNSLAAQNTENGDSESESDLRVARKNWHANGYKSQIPATSKTKFLKIASSEEDSKSHDSDNGHSRTAGPSTSLLKLKAESISEEEEKANSETRKYSSRKYSTGRKNAPFLKKAKLFSDSEDSESDKEDGQDRCHKMEMNPSSGNVECEPAARSQCFSDHVSETDLDSDDDKKAKEKPNSFIKDSASQNHGQSRKAARKRVCSSDSDSNSKVVKKASRARPGLLRVPRRCATTAASRIKLMSDEEDGRVDHVCTRRKNARKKPLHIACAPAQKIDSNSEGDVNCEVPNEQSGCEGQPLDADSEGSAKSISQSLNEDSDSEGVLNSGHKNRHTRSLKTSVASSKTKNALIVSLEEDSKSHIPRGEIGRKCSSESTLVHKATAENNFEEELNYGLRRWNGRRLRTYGKAPFSKTKVVRDSQEAAETEVKRKRLQTELENVKASETTGSSKCGPDTSPKSDSDLGSATESDADCTDDSKTKKRKTKGKAKVVRKESVPRDREPNTKMRTCVHNQKDAVPMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMPLLRRFRSWKEKAQ

Target ORF information:

RefSeq Version	NM_001103174.2
Organism	Bos taurus(cattle)
Definition	Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103174.2

ORF Insert Sequence:

ATGGCGGAGC CCTCGCCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA ACTGTACTTC 
CTCATCGCCC GGTACCTATC AGCCGGCCCA TGTCGGAGAG CCGCCCAGGT GTTGGTGCAG 
GAGCTGGAGC AGTATCAGTT GCTGCCGAAG AGGTTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAATTGGT CTTGTCCAAT AAGCATGTGG CTCCTGACCA TCTGTTGCAA 
ATCTGCCAAC GCATCGGTCC CATGCTGGAT AAAGAGATCC CACCCAGCAT TTCAAGAGTC 
ACTTCTTTAC TTGGGGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCG 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATCCATC GAGGAAAACA GCTCACAGGG 
TGTTCCACTT TTAGCACAGC ATTTCCAGGA GCTATGTATC AGCACATAAA AATGCACAGG 
AGGATTCTCG GACATCTGTC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA TTGTTTGGTG AAGATTTGGT CCACACACAA TGGCCGCCTA 
TTGTCCACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCAGGGAG CTGTGATAAA ATAATTAGAG TGTGGTGCCT GAGAACCTGT 
GCTCCAGTTG CTGTGCTCCA AGGACACACG GGGTCAATTA CATCTTTACA GTTTAGCCCC 
ATGGCCAAAG GCTCGCAAAG ATACATGGTC TCCACTGGTG CTGATGGGAC TGTTTGTTTT 
TGGCAGTGGG ATTTAGAATC CCTGAAATTC AGTCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGACCAG GTGTTCAAAT GCTGTGTTCT TCATTTAGTG TTGGTGGTAT GTTTCTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCTGAAAAA 
ATCGCAGAAC TTGAAAGTCA CACTGATAAA GTTGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCT TAAGTGGTAG CAGAGATGGA ACAGCACGAA TTTGGAGATT TGAACAGTTA 
GAATGGAGAA GCATCTTACT AGATATGGCC ACTAGAATCT CAGGAGATTT ATCTTTGGAA 
GAGGAAAGGT TTATGAAACC CAAAGTAACA ATGATAGCTT GGAATCAGAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAGCTG 
CTTCATAACC TGTTGGGACA TGCTGATGAA GTATTTGTTT TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAC GGCAGCATAT TCATATGGGA TATCACAAAA 
GGCACCAAGA TGAAACATTA TTTCAATATG ATTGAAGGAC AAGGACATGG AGCTGTCTTT 
GATTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGTCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAGCCA TATGAAAAGA TTCCTGACCA GATGTTCTTC 
CATACTGACT ATCGACCGCT GATTAGGGAT TCTAATAATT ACGTGTTAGA CGAGCAGACT 
CAGCAGGCCC CTCACCTTAT GCCTCCACCG TTCTTGGTAG ATGTAGATGG AAACCCTCAT 
CCAACTAAGT ATCAGCGATT AGTGCCTGGC CGAGAAAATT CTGCAGATGA ACATTTGGTT 
CCACAACTAG GCTATGTGGC AACAAGTGAT GGAGAGGTAA TAGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGGTGA ACGAAGCCCA GAGCGCAGTG TCCTGGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGGTC AGGAGGCTGT TCCAAGTGGA 
CTTTCAAATG GGGAAGAAAC ACCCCGAAGA GGTTTTAGAA GACTAAGCTT AGACATCCAG 
TCTCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTGG AAGGTGTCCG TCAGATGCAC 
CAGAATGCTC CGCGCAGTCA GATGGCTACG GAGCGTGACC TGCAGGCTTG GAAGCGAAGA 
GTGGTTGTCC CAGAGGTACC ACTAGGCATA TTTAGGAAGT TGGAAGACTT CAGAATAGAG 
AAAGGTGAAC AAGAAAGAAA TCTTTATATA ATTGGAAGAA AGAGGAAGAC TCTCCAGTTC 
TCACAAAAGT CTGATTCGGT GACTTTTATG TCACAGTCTA GACAGAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCTT GGACGGCTTG AGTTGTCTTC TGGAAATGAA 
TCTTCAAGCT CCATAAGACA TGAGACTTCT TGTGATCAAA GTGAAGGTTC TTGTTCTTCT 
GAAGAAGAAG AATGGAAAAG TGATAGAAGG AGTGAAAGTG ACAGTGAAAG TTCAAGTGAC 
TCTTCCTCTA GGTATTCTGA TTGGACAGCT GACGCAGGCA TCCACCTGCA GCCTCCTTTA 
CGAACATCAT CTCGTCGACG AATTACTCGA TTTTGCAGCA GTTCAGAGGA TGAAATGTCT 
GCTGAGAATT TATCTCCTCC AAAAAGGAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAA 
AAAGAGAATT TGCGGAGGAT GACTCAGGCA GAGCTTGCAG ACGTGGAACA TTTATATGAA 
TTTCATCCTC CAGTTTGGAT AACTGACACC ACACTTCGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTCAGA 
AGAAATAACA TTTATGAACT GAACCCTAAC AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTCAAAAT AGTTGGAATA CGATATGAGG TTGGACCCCC GACACTTTGT 
TGCCTCAAAC TAGCATTTAT AGATCCTGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATACC ATGACATGCC AGATGTTATT GACTTTCTTG TCTTGCGTCA GTTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGGTTCC GTTCCATCAT TGATGATGCT 
TGGTGGTTTG GAACGGTGCT ACGTCAAGAG CCGTATCAGC CACAGTACCC TGATAGTCAT 
TTCCAGTGTT ACATTGTGAG GTGGGACAAT ACTGAAATTG AAAAACTTAG CCCATGGGAT 
ATGGAACCAA TACCTGACAA TGTTGATCCA CCTGAAGAAT TAGGCGCTAG TATTTCTGTC 
ACTTCAGATG AGCTAGAGAA ATTGCTCTAC AAACCCCAAG ATGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA ACGAATTATT AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATTGCAGCAG CTTTTGCAGG TCCGGTTGAT CTGTGTACAT ATCCGAAGTA CTGTACTGTA 
GTGGCTTACC CAACTGATCT GTACACCATT CGAATGAGAC TTGTGAATCG ATTTTACAGG 
AGGCTATCTG CGTTAGTTTG GGAAGTCAGA TACATAGAAC ACAATGCCAG AACATTTAAT 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAGAAGATAA CAGACCAGCT TTTAAAATTT 
ATCAAGAATC AAGACTGTAT AAATATCTCA GAACTTTCTA ACACTTCTGA AAATGATGAG 
CAAAATGCCG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGAAGGAGA 
GTTCATGATT GGAAAAAAAG GAGCATCAAA ACAACCAATT ATGTTGAAAA CAACTGGAAG 
AAACAGTGTA AGGAACTAGT GAACTTAATT TTTCAATGTG AAGATTCTGA ACCATTTAGA 
CAACCTGTTG ATTTGGTTGA ATATCCAGAT TACCGAGACA TTATAGATAC TCCAATGGAT 
TTTGGAACAG TAAGGGAAAC TTTAGAAGCG GGAAACTATG ACAGCCCTTT GGAATTTTGC 
AAAGACATCC GTTTGATATT TAGCAATGCA AAAGCATATA CACCAAACAA AAGATCAAAG 
ATTTACAGTA TGACATTGAG ATTGTCTGCC TTATTTGAAG AAAAAATGAA GAAAATCTCC 
TCTGATTTTA AAATTGGTCA AAAATTCAGT GAAAAACTTC GAAGAAGCCA GAGGTTTAAG 
AGACGACAAA ATTGTAACCG TGTCAATCAG GCTAACAAAA GTATCAGAAA TACCAAGCAG 
AAGCGTTTAA AATCTCAGAC AAAAGTAATT TCTGAGTTCG TAGGTTCTCC TATCCAGCCT 
ACCTCAAGTA GGGCAACTTA CTCCGCAAGC CACAAGACAA CTGCTAGTGT CTCTTCAGGT 
GTTACTTCTG GTGACTCTTC AGATTCAGCA GCATCATCAG AAAGAATGAG AAGAAATAGA 
CCTGTCACTC TGACAAATGG TTCTACATTA TCTGAAAGTG AAATGGAAGA TTCTTTAGGT 
ACCTCTTCAT CATCTTCAGC TTCAAATAGT TCTGAGGAAA GCAAGGAGAG TCCAAGAGCT 
CGAGACTCCT CCTCCCGTAG TGGGCTAGCC AGGAGCAGCA ACCTCAGGGT GACCAGGACC 
AGAGCTGCAC AGAGAAAAAC TGGTCCAGTT TCTTTAGAAA ATGGATGTGG CAGAAAAGCC 
ACTAGGAAGA GAGTCTATTT AAGTGACTCA GATAACAATT CTTTGGCGAC TGGTGAAAGT 
GTAAAAGTGA GAGCTGGAAA TAACCGGAAA GTGCTACGAA AATGTGCTGC TGTGGCTGCC 
AATAAAATCA AGTTAATGAG TGATGTAGAG GAGAATTCTA CCTCTGAAAG TGTCTGTTCT 
GGCCGTAAGC TGCCCCACCG CAATGCTTCT GCTGTAGCTA GAAAAAAATT ATTACATAAT 
TCTGAGGATG ACCAGAGCTT GAAGTCAGAA CTTGAAGAAG AGGAGCTAAA AGATCAACTA 
TCACCATTGT CCAACTCTCT TGCTGCCCAG AATACTGAAA ATGGAGATTC AGAGTCAGAA 
AGTGATTTAC GGGTAGCCCG GAAAAATTGG CATGCTAATG GTTACAAGTC CCAAATTCCA 
GCAACTTCTA AAACAAAATT CCTTAAAATA GCATCTTCTG AGGAAGACTC TAAAAGTCAT 
GATTCAGATA ATGGACATAG CAGAACTGCT GGCCCATCAA CATCTTTGCT GAAACTCAAG 
GCAGAAAGCA TTTCAGAGGA AGAGGAGAAA GCAAATTCTG AAACAAGAAA ATACAGCAGT 
AGGAAATACA GCACAGGTCG CAAGAATGCT CCTTTCCTTA AAAAAGCAAA GCTCTTCAGT 
GATTCAGAGG ACTCTGAGTC TGACAAAGAA GATGGACAAG ACAGATGTCA TAAGATGGAA 
ATGAACCCGA GTTCAGGGAA TGTGGAATGT GAACCTGCTG CCAGATCCCA GTGTTTTTCA 
GATCATGTAT CTGAAACTGA TTTGGATTCA GATGATGACA AGAAAGCAAA AGAAAAACCA 
AATAGTTTTA TAAAAGATTC TGCATCACAA AATCATGGAC AGAGCAGGAA AGCTGCCAGG 
AAAAGGGTCT GTTCCAGTGA CTCAGACAGC AATTCGAAGG TGGTTAAGAA AGCATCCAGA 
GCCAGACCAG GCCTTCTGAG AGTGCCTCGG AGGTGTGCCA CCACTGCTGC CAGTCGGATC 
AAGCTGATGA GTGATGAGGA AGATGGCCGT GTAGACCACG TGTGCACCAG GCGCAAAAAT 
GCAAGGAAAA AGCCCCTCCA TATTGCATGT GCCCCAGCTC AGAAGATAGA CAGCAATTCT 
GAGGGGGATG TAAACTGTGA AGTGCCAAAC GAACAGAGTG GCTGTGAAGG GCAGCCACTT 
GATGCCGACT CAGAAGGTAG TGCAAAAAGT ATCAGTCAGT CTTTAAATGA AGACTCAGAC 
TCTGAAGGTG TGTTGAATTC AGGACACAAG AACAGGCATA CCAGGAGTCT CAAAACCAGT 
GTTGCATCTT CTAAAACAAA GAATGCCTTG ATTGTGTCTT TGGAGGAAGA TTCAAAAAGC 
CATATTCCAC GGGGTGAGAT AGGTAGAAAA TGTTCTTCTG AGTCAACTTT GGTGCACAAA 
GCTACTGCAG AGAATAACTT TGAAGAGGAA CTGAACTATG GGCTACGTAG GTGGAACGGC 
AGGAGGCTCC GGACCTATGG GAAGGCTCCT TTCAGTAAGA CAAAAGTGGT GCGGGATTCG 
CAGGAAGCTG CCGAGACAGA AGTAAAAAGG AAGAGACTGC AAACTGAATT GGAAAATGTG 
AAAGCTTCCG AAACAACAGG GAGTTCAAAG TGTGGACCAG ACACTAGCCC CAAATCTGAC 
TCAGATTTGG GATCTGCCAC TGAATCAGAT GCTGACTGTA CAGATGATTC AAAAACCAAA 
AAGAGGAAAA CGAAAGGGAA AGCAAAAGTA GTTAGAAAAG AATCTGTTCC TAGAGACAGA 
GAACCCAATA CAAAAATGAG AACATGTGTG CATAATCAGA AGGATGCAGT GCCAATGCCT 
AGTGAGACTC TGAAAGCAAA AATGGTACCT GAGAAAGTTC CCCGCAGATG TGCTACTGTT 
GCTGCAAATA AAATAAAGAT AATGAGTAAT CTAAAGGAAA CCATTTCAGG ACCAGAAAAT 
GTGTGGATTA GGAAGAGCAG CAGAAAACTG CCCCATCGAA ATGCTTCTGC TGCGGCTAAG 
AAAAAATTAC TCAATGTTTA TAAAGAGGAT GATACAACCA TAAATTCTGA AAGTGAAAAG 
GAGCTAGAAG ATATTAATAG AAAAATGCCC CTTTTAAGGC GTTTCAGATC CTGGAAAGAA 
AAAGCACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( NM_001103174.1 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_001096644.1 Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), mRNA.

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CloneID	OBa40619
Clone ID Related Accession (Same CDS sequence)	NM_001103174.2 , NM_001103174.1
Accession Version	NM_001103174.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1420214400000
Organism	Bos taurus(Cattle)
Product	bromodomain and WD repeat-containing protein 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC150984.8 and AC150879.3. On Sep 7, 2007 this sequence version replaced XM_612555.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGAGC CCTCGCCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA ACTGTACTTC 
CTCATCGCCC GGTACCTATC AGCCGGCCCA TGTCGGAGAG CCGCCCAGGT GTTGGTGCAG 
GAGCTGGAGC AGTATCAGTT GCTGCCGAAG AGGTTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAATTGGT CTTGTCCAAT AAGCATGTGG CTCCTGACCA TCTGTTGCAA 
ATCTGCCAAC GCATCGGTCC CATGCTGGAT AAAGAGATCC CACCCAGCAT TTCAAGAGTC 
ACTTCTTTAC TTGGGGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCG 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATCCATC GAGGAAAACA GCTCACAGGG 
TGTTCCACTT TTAGCACAGC ATTTCCAGGA GCTATGTATC AGCACATAAA AATGCACAGG 
AGGATTCTCG GACATCTGTC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA TTGTTTGGTG AAGATTTGGT CCACACACAA TGGCCGCCTA 
TTGTCCACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCAGGGAG CTGTGATAAA ATAATTAGAG TGTGGTGCCT GAGAACCTGT 
GCTCCAGTTG CTGTGCTCCA AGGACACACG GGGTCAATTA CATCTTTACA GTTTAGCCCC 
ATGGCCAAAG GCTCGCAAAG ATACATGGTC TCCACTGGTG CTGATGGGAC TGTTTGTTTT 
TGGCAGTGGG ATTTAGAATC CCTGAAATTC AGTCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGACCAG GTGTTCAAAT GCTGTGTTCT TCATTTAGTG TTGGTGGTAT GTTTCTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCTGAAAAA 
ATCGCAGAAC TTGAAAGTCA CACTGATAAA GTTGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCT TAAGTGGTAG CAGAGATGGA ACAGCACGAA TTTGGAGATT TGAACAGTTA 
GAATGGAGAA GCATCTTACT AGATATGGCC ACTAGAATCT CAGGAGATTT ATCTTTGGAA 
GAGGAAAGGT TTATGAAACC CAAAGTAACA ATGATAGCTT GGAATCAGAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAGCTG 
CTTCATAACC TGTTGGGACA TGCTGATGAA GTATTTGTTT TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAC GGCAGCATAT TCATATGGGA TATCACAAAA 
GGCACCAAGA TGAAACATTA TTTCAATATG ATTGAAGGAC AAGGACATGG AGCTGTCTTT 
GATTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGTCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAGCCA TATGAAAAGA TTCCTGACCA GATGTTCTTC 
CATACTGACT ATCGACCGCT GATTAGGGAT TCTAATAATT ACGTGTTAGA CGAGCAGACT 
CAGCAGGCCC CTCACCTTAT GCCTCCACCG TTCTTGGTAG ATGTAGATGG AAACCCTCAT 
CCAACTAAGT ATCAGCGATT AGTGCCTGGC CGAGAAAATT CTGCAGATGA ACATTTGGTT 
CCACAACTAG GCTATGTGGC AACAAGTGAT GGAGAGGTAA TAGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGGTGA ACGAAGCCCA GAGCGCAGTG TCCTGGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGGTC AGGAGGCTGT TCCAAGTGGA 
CTTTCAAATG GGGAAGAAAC ACCCCGAAGA GGTTTTAGAA GACTAAGCTT AGACATCCAG 
TCTCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTGG AAGGTGTCCG TCAGATGCAC 
CAGAATGCTC CGCGCAGTCA GATGGCTACG GAGCGTGACC TGCAGGCTTG GAAGCGAAGA 
GTGGTTGTCC CAGAGGTACC ACTAGGCATA TTTAGGAAGT TGGAAGACTT CAGAATAGAG 
AAAGGTGAAC AAGAAAGAAA TCTTTATATA ATTGGAAGAA AGAGGAAGAC TCTCCAGTTC 
TCACAAAAGT CTGATTCGGT GACTTTTATG TCACAGTCTA GACAGAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCTT GGACGGCTTG AGTTGTCTTC TGGAAATGAA 
TCTTCAAGCT CCATAAGACA TGAGACTTCT TGTGATCAAA GTGAAGGTTC TTGTTCTTCT 
GAAGAAGAAG AATGGAAAAG TGATAGAAGG AGTGAAAGTG ACAGTGAAAG TTCAAGTGAC 
TCTTCCTCTA GGTATTCTGA TTGGACAGCT GACGCAGGCA TCCACCTGCA GCCTCCTTTA 
CGAACATCAT CTCGTCGACG AATTACTCGA TTTTGCAGCA GTTCAGAGGA TGAAATGTCT 
GCTGAGAATT TATCTCCTCC AAAAAGGAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAA 
AAAGAGAATT TGCGGAGGAT GACTCAGGCA GAGCTTGCAG ACGTGGAACA TTTATATGAA 
TTTCATCCTC CAGTTTGGAT AACTGACACC ACACTTCGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTCAGA 
AGAAATAACA TTTATGAACT GAACCCTAAC AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTCAAAAT AGTTGGAATA CGATATGAGG TTGGACCCCC GACACTTTGT 
TGCCTCAAAC TAGCATTTAT AGATCCTGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATACC ATGACATGCC AGATGTTATT GACTTTCTTG TCTTGCGTCA GTTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGGTTCC GTTCCATCAT TGATGATGCT 
TGGTGGTTTG GAACGGTGCT ACGTCAAGAG CCGTATCAGC CACAGTACCC TGATAGTCAT 
TTCCAGTGTT ACATTGTGAG GTGGGACAAT ACTGAAATTG AAAAACTTAG CCCATGGGAT 
ATGGAACCAA TACCTGACAA TGTTGATCCA CCTGAAGAAT TAGGCGCTAG TATTTCTGTC 
ACTTCAGATG AGCTAGAGAA ATTGCTCTAC AAACCCCAAG ATGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA ACGAATTATT AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATTGCAGCAG CTTTTGCAGG TCCGGTTGAT CTGTGTACAT ATCCGAAGTA CTGTACTGTA 
GTGGCTTACC CAACTGATCT GTACACCATT CGAATGAGAC TTGTGAATCG ATTTTACAGG 
AGGCTATCTG CGTTAGTTTG GGAAGTCAGA TACATAGAAC ACAATGCCAG AACATTTAAT 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAGAAGATAA CAGACCAGCT TTTAAAATTT 
ATCAAGAATC AAGACTGTAT AAATATCTCA GAACTTTCTA ACACTTCTGA AAATGATGAG 
CAAAATGCCG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGAAGGAGA 
GTTCATGATT GGAAAAAAAG GAGCATCAAA ACAACCAATT ATGTTGAAAA CAACTGGAAG 
AAACAGTGTA AGGAACTAGT GAACTTAATT TTTCAATGTG AAGATTCTGA ACCATTTAGA 
CAACCTGTTG ATTTGGTTGA ATATCCAGAT TACCGAGACA TTATAGATAC TCCAATGGAT 
TTTGGAACAG TAAGGGAAAC TTTAGAAGCG GGAAACTATG ACAGCCCTTT GGAATTTTGC 
AAAGACATCC GTTTGATATT TAGCAATGCA AAAGCATATA CACCAAACAA AAGATCAAAG 
ATTTACAGTA TGACATTGAG ATTGTCTGCC TTATTTGAAG AAAAAATGAA GAAAATCTCC 
TCTGATTTTA AAATTGGTCA AAAATTCAGT GAAAAACTTC GAAGAAGCCA GAGGTTTAAG 
AGACGACAAA ATTGTAACCG TGTCAATCAG GCTAACAAAA GTATCAGAAA TACCAAGCAG 
AAGCGTTTAA AATCTCAGAC AAAAGTAATT TCTGAGTTCG TAGGTTCTCC TATCCAGCCT 
ACCTCAAGTA GGGCAACTTA CTCCGCAAGC CACAAGACAA CTGCTAGTGT CTCTTCAGGT 
GTTACTTCTG GTGACTCTTC AGATTCAGCA GCATCATCAG AAAGAATGAG AAGAAATAGA 
CCTGTCACTC TGACAAATGG TTCTACATTA TCTGAAAGTG AAATGGAAGA TTCTTTAGGT 
ACCTCTTCAT CATCTTCAGC TTCAAATAGT TCTGAGGAAA GCAAGGAGAG TCCAAGAGCT 
CGAGACTCCT CCTCCCGTAG TGGGCTAGCC AGGAGCAGCA ACCTCAGGGT GACCAGGACC 
AGAGCTGCAC AGAGAAAAAC TGGTCCAGTT TCTTTAGAAA ATGGATGTGG CAGAAAAGCC 
ACTAGGAAGA GAGTCTATTT AAGTGACTCA GATAACAATT CTTTGGCGAC TGGTGAAAGT 
GTAAAAGTGA GAGCTGGAAA TAACCGGAAA GTGCTACGAA AATGTGCTGC TGTGGCTGCC 
AATAAAATCA AGTTAATGAG TGATGTAGAG GAGAATTCTA CCTCTGAAAG TGTCTGTTCT 
GGCCGTAAGC TGCCCCACCG CAATGCTTCT GCTGTAGCTA GAAAAAAATT ATTACATAAT 
TCTGAGGATG ACCAGAGCTT GAAGTCAGAA CTTGAAGAAG AGGAGCTAAA AGATCAACTA 
TCACCATTGT CCAACTCTCT TGCTGCCCAG AATACTGAAA ATGGAGATTC AGAGTCAGAA 
AGTGATTTAC GGGTAGCCCG GAAAAATTGG CATGCTAATG GTTACAAGTC CCAAATTCCA 
GCAACTTCTA AAACAAAATT CCTTAAAATA GCATCTTCTG AGGAAGACTC TAAAAGTCAT 
GATTCAGATA ATGGACATAG CAGAACTGCT GGCCCATCAA CATCTTTGCT GAAACTCAAG 
GCAGAAAGCA TTTCAGAGGA AGAGGAGAAA GCAAATTCTG AAACAAGAAA ATACAGCAGT 
AGGAAATACA GCACAGGTCG CAAGAATGCT CCTTTCCTTA AAAAAGCAAA GCTCTTCAGT 
GATTCAGAGG ACTCTGAGTC TGACAAAGAA GATGGACAAG ACAGATGTCA TAAGATGGAA 
ATGAACCCGA GTTCAGGGAA TGTGGAATGT GAACCTGCTG CCAGATCCCA GTGTTTTTCA 
GATCATGTAT CTGAAACTGA TTTGGATTCA GATGATGACA AGAAAGCAAA AGAAAAACCA 
AATAGTTTTA TAAAAGATTC TGCATCACAA AATCATGGAC AGAGCAGGAA AGCTGCCAGG 
AAAAGGGTCT GTTCCAGTGA CTCAGACAGC AATTCGAAGG TGGTTAAGAA AGCATCCAGA 
GCCAGACCAG GCCTTCTGAG AGTGCCTCGG AGGTGTGCCA CCACTGCTGC CAGTCGGATC 
AAGCTGATGA GTGATGAGGA AGATGGCCGT GTAGACCACG TGTGCACCAG GCGCAAAAAT 
GCAAGGAAAA AGCCCCTCCA TATTGCATGT GCCCCAGCTC AGAAGATAGA CAGCAATTCT 
GAGGGGGATG TAAACTGTGA AGTGCCAAAC GAACAGAGTG GCTGTGAAGG GCAGCCACTT 
GATGCCGACT CAGAAGGTAG TGCAAAAAGT ATCAGTCAGT CTTTAAATGA AGACTCAGAC 
TCTGAAGGTG TGTTGAATTC AGGACACAAG AACAGGCATA CCAGGAGTCT CAAAACCAGT 
GTTGCATCTT CTAAAACAAA GAATGCCTTG ATTGTGTCTT TGGAGGAAGA TTCAAAAAGC 
CATATTCCAC GGGGTGAGAT AGGTAGAAAA TGTTCTTCTG AGTCAACTTT GGTGCACAAA 
GCTACTGCAG AGAATAACTT TGAAGAGGAA CTGAACTATG GGCTACGTAG GTGGAACGGC 
AGGAGGCTCC GGACCTATGG GAAGGCTCCT TTCAGTAAGA CAAAAGTGGT GCGGGATTCG 
CAGGAAGCTG CCGAGACAGA AGTAAAAAGG AAGAGACTGC AAACTGAATT GGAAAATGTG 
AAAGCTTCCG AAACAACAGG GAGTTCAAAG TGTGGACCAG ACACTAGCCC CAAATCTGAC 
TCAGATTTGG GATCTGCCAC TGAATCAGAT GCTGACTGTA CAGATGATTC AAAAACCAAA 
AAGAGGAAAA CGAAAGGGAA AGCAAAAGTA GTTAGAAAAG AATCTGTTCC TAGAGACAGA 
GAACCCAATA CAAAAATGAG AACATGTGTG CATAATCAGA AGGATGCAGT GCCAATGCCT 
AGTGAGACTC TGAAAGCAAA AATGGTACCT GAGAAAGTTC CCCGCAGATG TGCTACTGTT 
GCTGCAAATA AAATAAAGAT AATGAGTAAT CTAAAGGAAA CCATTTCAGG ACCAGAAAAT 
GTGTGGATTA GGAAGAGCAG CAGAAAACTG CCCCATCGAA ATGCTTCTGC TGCGGCTAAG 
AAAAAATTAC TCAATGTTTA TAAAGAGGAT GATACAACCA TAAATTCTGA AAGTGAAAAG 
GAGCTAGAAG ATATTAATAG AAAAATGCCC CTTTTAAGGC GTTTCAGATC CTGGAAAGAA 
AAAGCACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096644.1
CDS	100..7071
Translation	MAEPSPARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGAMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSLEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLLGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLVPQLGYVATSDGEVIEQIISLQTNDNGERSPERSVLDGMIRQLQQQQDQRMGAGQEAVPSGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQMATERDLQAWKRRVVVPEVPLGIFRKLEDFRIEKGEQERNLYIIGRKRKTLQFSQKSDSVTFMSQSRQRTCRRKYPNYGRRNLGRLELSSGNESSSSIRHETSCDQSEGSCSSEEEEWKSDRRSESDSESSSDSSSRYSDWTADAGIHLQPPLRTSSRRRITRFCSSSEDEMSAENLSPPKRRRKRKKENKPKKENLRRMTQAELADVEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLRQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTSDELEKLLYKPQDGEWGQKSRDEECERIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQDCINISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDWKKRSIKTTNYVENNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLEAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFSEKLRRSQRFKRRQNCNRVNQANKSIRNTKQKRLKSQTKVISEFVGSPIQPTSSRATYSASHKTTASVSSGVTSGDSSDSAASSERMRRNRPVTLTNGSTLSESEMEDSLGTSSSSSASNSSEESKESPRARDSSSRSGLARSSNLRVTRTRAAQRKTGPVSLENGCGRKATRKRVYLSDSDNNSLATGESVKVRAGNNRKVLRKCAAVAANKIKLMSDVEENSTSESVCSGRKLPHRNASAVARKKLLHNSEDDQSLKSELEEEELKDQLSPLSNSLAAQNTENGDSESESDLRVARKNWHANGYKSQIPATSKTKFLKIASSEEDSKSHDSDNGHSRTAGPSTSLLKLKAESISEEEEKANSETRKYSSRKYSTGRKNAPFLKKAKLFSDSEDSESDKEDGQDRCHKMEMNPSSGNVECEPAARSQCFSDHVSETDLDSDDDKKAKEKPNSFIKDSASQNHGQSRKAARKRVCSSDSDSNSKVVKKASRARPGLLRVPRRCATTAASRIKLMSDEEDGRVDHVCTRRKNARKKPLHIACAPAQKIDSNSEGDVNCEVPNEQSGCEGQPLDADSEGSAKSISQSLNEDSDSEGVLNSGHKNRHTRSLKTSVASSKTKNALIVSLEEDSKSHIPRGEIGRKCSSESTLVHKATAENNFEEELNYGLRRWNGRRLRTYGKAPFSKTKVVRDSQEAAETEVKRKRLQTELENVKASETTGSSKCGPDTSPKSDSDLGSATESDADCTDDSKTKKRKTKGKAKVVRKESVPRDREPNTKMRTCVHNQKDAVPMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMPLLRRFRSWKEKAQ

Target ORF information:

RefSeq Version	NM_001103174.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103174.1

ORF Insert Sequence:

ATGGCGGAGC CCTCGCCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA ACTGTACTTC 
CTCATCGCCC GGTACCTATC AGCCGGCCCA TGTCGGAGAG CCGCCCAGGT GTTGGTGCAG 
GAGCTGGAGC AGTATCAGTT GCTGCCGAAG AGGTTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAATTGGT CTTGTCCAAT AAGCATGTGG CTCCTGACCA TCTGTTGCAA 
ATCTGCCAAC GCATCGGTCC CATGCTGGAT AAAGAGATCC CACCCAGCAT TTCAAGAGTC 
ACTTCTTTAC TTGGGGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCG 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATCCATC GAGGAAAACA GCTCACAGGG 
TGTTCCACTT TTAGCACAGC ATTTCCAGGA GCTATGTATC AGCACATAAA AATGCACAGG 
AGGATTCTCG GACATCTGTC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA TTGTTTGGTG AAGATTTGGT CCACACACAA TGGCCGCCTA 
TTGTCCACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCAGGGAG CTGTGATAAA ATAATTAGAG TGTGGTGCCT GAGAACCTGT 
GCTCCAGTTG CTGTGCTCCA AGGACACACG GGGTCAATTA CATCTTTACA GTTTAGCCCC 
ATGGCCAAAG GCTCGCAAAG ATACATGGTC TCCACTGGTG CTGATGGGAC TGTTTGTTTT 
TGGCAGTGGG ATTTAGAATC CCTGAAATTC AGTCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGACCAG GTGTTCAAAT GCTGTGTTCT TCATTTAGTG TTGGTGGTAT GTTTCTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCTGAAAAA 
ATCGCAGAAC TTGAAAGTCA CACTGATAAA GTTGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCT TAAGTGGTAG CAGAGATGGA ACAGCACGAA TTTGGAGATT TGAACAGTTA 
GAATGGAGAA GCATCTTACT AGATATGGCC ACTAGAATCT CAGGAGATTT ATCTTTGGAA 
GAGGAAAGGT TTATGAAACC CAAAGTAACA ATGATAGCTT GGAATCAGAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAGCTG 
CTTCATAACC TGTTGGGACA TGCTGATGAA GTATTTGTTT TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAC GGCAGCATAT TCATATGGGA TATCACAAAA 
GGCACCAAGA TGAAACATTA TTTCAATATG ATTGAAGGAC AAGGACATGG AGCTGTCTTT 
GATTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGTCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAGCCA TATGAAAAGA TTCCTGACCA GATGTTCTTC 
CATACTGACT ATCGACCGCT GATTAGGGAT TCTAATAATT ACGTGTTAGA CGAGCAGACT 
CAGCAGGCCC CTCACCTTAT GCCTCCACCG TTCTTGGTAG ATGTAGATGG AAACCCTCAT 
CCAACTAAGT ATCAGCGATT AGTGCCTGGC CGAGAAAATT CTGCAGATGA ACATTTGGTT 
CCACAACTAG GCTATGTGGC AACAAGTGAT GGAGAGGTAA TAGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGGTGA ACGAAGCCCA GAGCGCAGTG TCCTGGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGGTC AGGAGGCTGT TCCAAGTGGA 
CTTTCAAATG GGGAAGAAAC ACCCCGAAGA GGTTTTAGAA GACTAAGCTT AGACATCCAG 
TCTCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTGG AAGGTGTCCG TCAGATGCAC 
CAGAATGCTC CGCGCAGTCA GATGGCTACG GAGCGTGACC TGCAGGCTTG GAAGCGAAGA 
GTGGTTGTCC CAGAGGTACC ACTAGGCATA TTTAGGAAGT TGGAAGACTT CAGAATAGAG 
AAAGGTGAAC AAGAAAGAAA TCTTTATATA ATTGGAAGAA AGAGGAAGAC TCTCCAGTTC 
TCACAAAAGT CTGATTCGGT GACTTTTATG TCACAGTCTA GACAGAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCTT GGACGGCTTG AGTTGTCTTC TGGAAATGAA 
TCTTCAAGCT CCATAAGACA TGAGACTTCT TGTGATCAAA GTGAAGGTTC TTGTTCTTCT 
GAAGAAGAAG AATGGAAAAG TGATAGAAGG AGTGAAAGTG ACAGTGAAAG TTCAAGTGAC 
TCTTCCTCTA GGTATTCTGA TTGGACAGCT GACGCAGGCA TCCACCTGCA GCCTCCTTTA 
CGAACATCAT CTCGTCGACG AATTACTCGA TTTTGCAGCA GTTCAGAGGA TGAAATGTCT 
GCTGAGAATT TATCTCCTCC AAAAAGGAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAA 
AAAGAGAATT TGCGGAGGAT GACTCAGGCA GAGCTTGCAG ACGTGGAACA TTTATATGAA 
TTTCATCCTC CAGTTTGGAT AACTGACACC ACACTTCGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTCAGA 
AGAAATAACA TTTATGAACT GAACCCTAAC AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTCAAAAT AGTTGGAATA CGATATGAGG TTGGACCCCC GACACTTTGT 
TGCCTCAAAC TAGCATTTAT AGATCCTGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATACC ATGACATGCC AGATGTTATT GACTTTCTTG TCTTGCGTCA GTTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGGTTCC GTTCCATCAT TGATGATGCT 
TGGTGGTTTG GAACGGTGCT ACGTCAAGAG CCGTATCAGC CACAGTACCC TGATAGTCAT 
TTCCAGTGTT ACATTGTGAG GTGGGACAAT ACTGAAATTG AAAAACTTAG CCCATGGGAT 
ATGGAACCAA TACCTGACAA TGTTGATCCA CCTGAAGAAT TAGGCGCTAG TATTTCTGTC 
ACTTCAGATG AGCTAGAGAA ATTGCTCTAC AAACCCCAAG ATGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA ACGAATTATT AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATTGCAGCAG CTTTTGCAGG TCCGGTTGAT CTGTGTACAT ATCCGAAGTA CTGTACTGTA 
GTGGCTTACC CAACTGATCT GTACACCATT CGAATGAGAC TTGTGAATCG ATTTTACAGG 
AGGCTATCTG CGTTAGTTTG GGAAGTCAGA TACATAGAAC ACAATGCCAG AACATTTAAT 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAGAAGATAA CAGACCAGCT TTTAAAATTT 
ATCAAGAATC AAGACTGTAT AAATATCTCA GAACTTTCTA ACACTTCTGA AAATGATGAG 
CAAAATGCCG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGAAGGAGA 
GTTCATGATT GGAAAAAAAG GAGCATCAAA ACAACCAATT ATGTTGAAAA CAACTGGAAG 
AAACAGTGTA AGGAACTAGT GAACTTAATT TTTCAATGTG AAGATTCTGA ACCATTTAGA 
CAACCTGTTG ATTTGGTTGA ATATCCAGAT TACCGAGACA TTATAGATAC TCCAATGGAT 
TTTGGAACAG TAAGGGAAAC TTTAGAAGCG GGAAACTATG ACAGCCCTTT GGAATTTTGC 
AAAGACATCC GTTTGATATT TAGCAATGCA AAAGCATATA CACCAAACAA AAGATCAAAG 
ATTTACAGTA TGACATTGAG ATTGTCTGCC TTATTTGAAG AAAAAATGAA GAAAATCTCC 
TCTGATTTTA AAATTGGTCA AAAATTCAGT GAAAAACTTC GAAGAAGCCA GAGGTTTAAG 
AGACGACAAA ATTGTAACCG TGTCAATCAG GCTAACAAAA GTATCAGAAA TACCAAGCAG 
AAGCGTTTAA AATCTCAGAC AAAAGTAATT TCTGAGTTCG TAGGTTCTCC TATCCAGCCT 
ACCTCAAGTA GGGCAACTTA CTCCGCAAGC CACAAGACAA CTGCTAGTGT CTCTTCAGGT 
GTTACTTCTG GTGACTCTTC AGATTCAGCA GCATCATCAG AAAGAATGAG AAGAAATAGA 
CCTGTCACTC TGACAAATGG TTCTACATTA TCTGAAAGTG AAATGGAAGA TTCTTTAGGT 
ACCTCTTCAT CATCTTCAGC TTCAAATAGT TCTGAGGAAA GCAAGGAGAG TCCAAGAGCT 
CGAGACTCCT CCTCCCGTAG TGGGCTAGCC AGGAGCAGCA ACCTCAGGGT GACCAGGACC 
AGAGCTGCAC AGAGAAAAAC TGGTCCAGTT TCTTTAGAAA ATGGATGTGG CAGAAAAGCC 
ACTAGGAAGA GAGTCTATTT AAGTGACTCA GATAACAATT CTTTGGCGAC TGGTGAAAGT 
GTAAAAGTGA GAGCTGGAAA TAACCGGAAA GTGCTACGAA AATGTGCTGC TGTGGCTGCC 
AATAAAATCA AGTTAATGAG TGATGTAGAG GAGAATTCTA CCTCTGAAAG TGTCTGTTCT 
GGCCGTAAGC TGCCCCACCG CAATGCTTCT GCTGTAGCTA GAAAAAAATT ATTACATAAT 
TCTGAGGATG ACCAGAGCTT GAAGTCAGAA CTTGAAGAAG AGGAGCTAAA AGATCAACTA 
TCACCATTGT CCAACTCTCT TGCTGCCCAG AATACTGAAA ATGGAGATTC AGAGTCAGAA 
AGTGATTTAC GGGTAGCCCG GAAAAATTGG CATGCTAATG GTTACAAGTC CCAAATTCCA 
GCAACTTCTA AAACAAAATT CCTTAAAATA GCATCTTCTG AGGAAGACTC TAAAAGTCAT 
GATTCAGATA ATGGACATAG CAGAACTGCT GGCCCATCAA CATCTTTGCT GAAACTCAAG 
GCAGAAAGCA TTTCAGAGGA AGAGGAGAAA GCAAATTCTG AAACAAGAAA ATACAGCAGT 
AGGAAATACA GCACAGGTCG CAAGAATGCT CCTTTCCTTA AAAAAGCAAA GCTCTTCAGT 
GATTCAGAGG ACTCTGAGTC TGACAAAGAA GATGGACAAG ACAGATGTCA TAAGATGGAA 
ATGAACCCGA GTTCAGGGAA TGTGGAATGT GAACCTGCTG CCAGATCCCA GTGTTTTTCA 
GATCATGTAT CTGAAACTGA TTTGGATTCA GATGATGACA AGAAAGCAAA AGAAAAACCA 
AATAGTTTTA TAAAAGATTC TGCATCACAA AATCATGGAC AGAGCAGGAA AGCTGCCAGG 
AAAAGGGTCT GTTCCAGTGA CTCAGACAGC AATTCGAAGG TGGTTAAGAA AGCATCCAGA 
GCCAGACCAG GCCTTCTGAG AGTGCCTCGG AGGTGTGCCA CCACTGCTGC CAGTCGGATC 
AAGCTGATGA GTGATGAGGA AGATGGCCGT GTAGACCACG TGTGCACCAG GCGCAAAAAT 
GCAAGGAAAA AGCCCCTCCA TATTGCATGT GCCCCAGCTC AGAAGATAGA CAGCAATTCT 
GAGGGGGATG TAAACTGTGA AGTGCCAAAC GAACAGAGTG GCTGTGAAGG GCAGCCACTT 
GATGCCGACT CAGAAGGTAG TGCAAAAAGT ATCAGTCAGT CTTTAAATGA AGACTCAGAC 
TCTGAAGGTG TGTTGAATTC AGGACACAAG AACAGGCATA CCAGGAGTCT CAAAACCAGT 
GTTGCATCTT CTAAAACAAA GAATGCCTTG ATTGTGTCTT TGGAGGAAGA TTCAAAAAGC 
CATATTCCAC GGGGTGAGAT AGGTAGAAAA TGTTCTTCTG AGTCAACTTT GGTGCACAAA 
GCTACTGCAG AGAATAACTT TGAAGAGGAA CTGAACTATG GGCTACGTAG GTGGAACGGC 
AGGAGGCTCC GGACCTATGG GAAGGCTCCT TTCAGTAAGA CAAAAGTGGT GCGGGATTCG 
CAGGAAGCTG CCGAGACAGA AGTAAAAAGG AAGAGACTGC AAACTGAATT GGAAAATGTG 
AAAGCTTCCG AAACAACAGG GAGTTCAAAG TGTGGACCAG ACACTAGCCC CAAATCTGAC 
TCAGATTTGG GATCTGCCAC TGAATCAGAT GCTGACTGTA CAGATGATTC AAAAACCAAA 
AAGAGGAAAA CGAAAGGGAA AGCAAAAGTA GTTAGAAAAG AATCTGTTCC TAGAGACAGA 
GAACCCAATA CAAAAATGAG AACATGTGTG CATAATCAGA AGGATGCAGT GCCAATGCCT 
AGTGAGACTC TGAAAGCAAA AATGGTACCT GAGAAAGTTC CCCGCAGATG TGCTACTGTT 
GCTGCAAATA AAATAAAGAT AATGAGTAAT CTAAAGGAAA CCATTTCAGG ACCAGAAAAT 
GTGTGGATTA GGAAGAGCAG CAGAAAACTG CCCCATCGAA ATGCTTCTGC TGCGGCTAAG 
AAAAAATTAC TCAATGTTTA TAAAGAGGAT GATACAACCA TAAATTCTGA AAGTGAAAAG 
GAGCTAGAAG ATATTAATAG AAAAATGCCC CTTTTAAGGC GTTTCAGATC CTGGAAAGAA 
AAAGCACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( XM_024991350.1 ) cDNA ORF clone, Bos taurus(Cattle) -> XP_024847118.1 Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X1, mRNA.

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CloneID	OBa218889
Clone ID Related Accession (Same CDS sequence)	XM_024991350.1
Accession Version	XM_024991350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	bromodomain and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGATA AAGAGATCCC ACCCAGCATT TCAAGAGTCA CTTCTTTACT TGGGGCAGGA 
AGGCAGTCTT TGCTACGTAC AGCAAAAGAC TGCAGGCACA CAGTTTGGAA GGGCTCTGCC 
TTTGCTGCTC TTCATAGAGG AAGACCTCCT GAAATGCCGG TGAATTATGG TTCCCCACCA 
AATCTTGTGG AGATCCATCG AGGAAAACAG CTCACAGGGT GTTCCACTTT TAGCACAGCA 
TTTCCAGGAG CTATGTATCA GCACATAAAA ATGCACAGGA GGATTCTCGG ACATCTGTCT 
GCTGTTTACT GTGTAGCATT TGATAGGACA GGACATAGAA TCTTTACAGG TTCAGATGAT 
TGTTTGGTGA AGATTTGGTC CACACACAAT GGCCGCCTAT TGTCCACATT AAGAGGTCAT 
TCTGCAGAAA TTTCAGATAT GGCAGTAAAC TATGAGAATA CAATGATTGC TGCAGGGAGC 
TGTGATAAAA TAATTAGAGT GTGGTGCCTG AGAACCTGTG CTCCAGTTGC TGTGCTCCAA 
GGACACACGG GGTCAATTAC ATCTTTACAG TTTAGCCCCA TGGCCAAAGG CTCGCAAAGA 
TACATGGTCT CCACTGGTGC TGATGGGACT GTTTGTTTTT GGCAGTGGGA TTTAGAATCC 
CTGAAATTCA GTCCACGTCC CCTGAAGTTC ACTGAAAAGC CTAGACCAGG TGTTCAAATG 
CTGTGTTCTT CATTTAGTGT TGGTGGTATG TTTCTAGCCA CAGGTAGTAC TGATCATGTA 
ATCAGAATGT ATTTTTTGGG TTTTGAAGCA CCTGAAAAAA TCGCAGAACT TGAAAGTCAC 
ACTGATAAAG TTGATAGTAT CCAATTTTGT AACAATGGTG ATCGGTTCTT AAGTGGTAGC 
AGAGATGGAA CAGCACGAAT TTGGAGATTT GAACAGTTAG AATGGAGAAG CATCTTACTA 
GATATGGCCA CTAGAATCTC AGGAGATTTA TCTTTGGAAG AGGAAAGGTT TATGAAACCC 
AAAGTAACAA TGATAGCTTG GAATCAGAAT GATAGCATTG TTGTCACAGC TGTGAATGAT 
CATGTCCTCA AAGTGTGGAA TTCTTACACT GGACAGCTGC TTCATAACCT GTTGGGACAT 
GCTGATGAAG TATTTGTTTT GGAGACACAT CCCTTTGATT CCAGAATTAT GTTATCTGCA 
GGACATGACG GCAGCATATT CATATGGGAT ATCACAAAAG GCACCAAGAT GAAACATTAT 
TTCAATATGA TTGAAGGACA AGGACATGGA GCTGTCTTTG ATTGTAAGTT TTCACAGGAT 
GGACAGCATT TTGCCTGTAC AGATTCTCAT GGTCACCTTC TGATATTTGG TTTTGGATGC 
AGCAAGCCAT ATGAAAAGAT TCCTGACCAG ATGTTCTTCC ATACTGACTA TCGACCGCTG 
ATTAGGGATT CTAATAATTA CGTGTTAGAC GAGCAGACTC AGCAGGCCCC TCACCTTATG 
CCTCCACCGT TCTTGGTAGA TGTAGATGGA AACCCTCATC CAACTAAGTA TCAGCGATTA 
GTGCCTGGCC GAGAAAATTC TGCAGATGAA CATTTGGTTC CACAACTAGG CTATGTGGCA 
ACAAGTGATG GAGAGGTAAT AGAACAAATT ATAAGCCTGC AAACCAATGA TAATGGTGAA 
CGAAGCCCAG AGCGCAGTGT CCTGGATGGA ATGATAAGAC AGTTGCAGCA GCAGCAAGAT 
CAGAGAATGG GAGCAGGTCA GGAGGCTGTT CCAAGTGGAC TTTCAAATGG GGAAGAAACA 
CCCCGAAGAG GTTTTAGAAG ACTAAGCTTA GACATCCAGT CTCCTCCAAA TATTGGTCTG 
CGTCGTAGTG GACAAGTGGA AGGTGTCCGT CAGATGCACC AGAATGCTCC GCGCAGTCAG 
ATGGCTACGG AGCGTGACCT GCAGGCTTGG AAGCGAAGAG TGGTTGTCCC AGAGGTACCA 
CTAGGCATAT TTAGGAAGTT GGAAGACTTC AGAATAGAGA AAGGTGAACA AGAAAGAAAT 
CTTTATATAA TTGGAAGAAA GAGGAAGACT CTCCAGTTCT CACAAAAGTC TGATTCGGTG 
ACTTTTATGT CACAGTCTAG ACAGAGGACA TGTAGGCGTA AATATCCAAA TTATGGTAGA 
AGAAATCTTG GACGGCTTGA GTTGTCTTCT GGAAATGAAT CTTCAAGCTC CATAAGACAT 
GAGACTTCTT GTGATCAAAG TGAAGGTTCT TGTTCTTCTG AAGAAGAAGA ATGGAAAAGT 
GATAGAAGGA GTGAAAGTGA CAGTGAAAGT TCAAGTGACT CTTCCTCTAG GTATTCTGAT 
TGGACAGCTG ACGCAGGCAT CCACCTGCAG CCTCCTTTAC GAACATCATC TCGTCGACGA 
ATTACTCGAT TTTGCAGCAG TTCAGAGGAT GAAATGTCTG CTGAGAATTT ATCTCCTCCA 
AAAAGGAGAC GAAAGAGAAA GAAAGAAAAT AAGCCTAAAA AAGAGAATTT GCGGAGGATG 
ACTCAGGCAG AGCTTGCAGA CGTGGAACAT TTATATGAAT TTCATCCTCC AGTTTGGATA 
ACTGACACCA CACTTCGAAA ATCTCCTTTT GTTCCTCAAA TGGGTGATGA GGTAATATAT 
TTTCGACAGG GTCATGAAGC TTATATTGAG GCTGTCAGAA GAAATAACAT TTATGAACTG 
AACCCTAACA AGGAGCCATG GAGAAAAATG GATCTTAGGG ATCAAGAATT GGTCAAAATA 
GTTGGAATAC GATATGAGGT TGGACCCCCG ACACTTTGTT GCCTCAAACT AGCATTTATA 
GATCCTGCAA CTGGAAAACT TATGGACAAA TCTTTCTCTA TTAGATACCA TGACATGCCA 
GATGTTATTG ACTTTCTTGT CTTGCGTCAG TTTTATGATG AAGCAAGACA GAGGAATTGG 
CAGTCTTGTG ACAGGTTCCG TTCCATCATT GATGATGCTT GGTGGTTTGG AACGGTGCTA 
CGTCAAGAGC CGTATCAGCC ACAGTACCCT GATAGTCATT TCCAGTGTTA CATTGTGAGG 
TGGGACAATA CTGAAATTGA AAAACTTAGC CCATGGGATA TGGAACCAAT ACCTGACAAT 
GTTGATCCAC CTGAAGAATT AGGCGCTAGT ATTTCTGTCA CTTCAGATGA GCTAGAGAAA 
TTGCTCTACA AACCCCAAGA TGGTGAATGG GGTCAGAAAT CAAGAGATGA AGAATGTGAA 
CGAATTATTA GTGGTATAGA TCAACTTTTG AATCTTGATA TTGCAGCAGC TTTTGCAGGT 
CCGGTTGATC TGTGTACATA TCCGAAGTAC TGTACTGTAG TGGCTTACCC AACTGATCTG 
TACACCATTC GAATGAGACT TGTGAATCGA TTTTACAGGA GGCTATCTGC GTTAGTTTGG 
GAAGTCAGAT ACATAGAACA CAATGCCAGA ACATTTAATG AACCTGAGAG TGTAATTGCA 
AGATCAGCTA AGAAGATAAC AGACCAGCTT TTAAAATTTA TCAAGAATCA AGACTGTATA 
AATATCTCAG AACTTTCTAA CACTTCTGAA AATGATGAGC AAAATGCCGA GGATTTGGAT 
GATAGTGATC TTCCTAAAAC ATCTTCTGGA AGAAGGAGAG TTCATGATTG GAAAAAAAGG 
AGCATCAAAA CAACCAATTA TGTTGAAAAC AACTGGAAGA AACAGTGTAA GGAACTAGTG 
AACTTAATTT TTCAATGTGA AGATTCTGAA CCATTTAGAC AACCTGTTGA TTTGGTTGAA 
TATCCAGATT ACCGAGACAT TATAGATACT CCAATGGATT TTGGAACAGT AAGGGAAACT 
TTAGAAGCGG GAAACTATGA CAGCCCTTTG GAATTTTGCA AAGACATCCG TTTGATATTT 
AGCAATGCAA AAGCATATAC ACCAAACAAA AGATCAAAGA TTTACAGTAT GACATTGAGA 
TTGTCTGCCT TATTTGAAGA AAAAATGAAG AAAATCTCCT CTGATTTTAA AATTGGTCAA 
AAATTCAGTG AAAAACTTCG AAGAAGCCAG AGGTTTAAGA GACGACAAAA TTGTAACCGT 
GTCAATCAGG CTAACAAAAG TATCAGAAAT ACCAAGCAGA AGCGTTTAAA ATCTCAGACA 
AAAGTAATTT CTGAGTTCGT AGGTTCTCCT ATCCAGCCTA CCTCAAGTAG GGCAACTTAC 
TCCGCAAGCC ACAAGACAAC TGCTAGTGTC TCTTCAGGTG TTACTTCTGG TGACTCTTCA 
GATTCAGCAG CATCATCAGA AAGAATGAGA AGAAATAGAC CTGTCACTCT GACAAATGGT 
TCTACATTAT CTGAAAGTGA AATGGAAGAT TCTTTAGGTA CCTCTTCATC ATCTTCAGCT 
TCAAATAGTT CTGAGGAAAG CAAGGAGAGT CCAAGAGCTC GAGACTCCTC CTCCCGTAGT 
GGGCTAGCCA GGAGCAGCAA CCTCAGGGTG ACCAGGACCA GAGCTGCACA GAGAAAAACT 
GGTCCAGTTT CTTTAGAAAA TGGATGTGGC AGAAAAGCCA CTAGGAAGAG AGTCTATTTA 
AGTGACTCAG ATAACAATTC TTTGGCGACT GGTGAAAGTG TAAAAGTGAG AGCTGGAAAT 
AACCGGAAAG TGCTACGAAA ATGTGCTGCT GTGGCTGCCA ATAAAATCAA GTTAATGAGT 
GATGTAGAGG AGAATTCTAC CTCTGAAAGT GTCTGTTCTG GCCGTAAGCT GCCCCACCGC 
AATGCTTCTG CTGTAGCTAG AAAAAAATTA TTACATAATT CTGAGGATGA CCAGAGCTTG 
AAGTCAGAAC TTGAAGAAGA GGAGCTAAAA GATCAACTAT CACCATTGTC CAACTCTCTT 
GCTGCCCAGA ATACTGAAAA TGGAGATTCA GAGTCAGAAA GTGATTTACG GGTAGCCCGG 
AAAAATTGGC ATGCTAATGG TTACAAGTCC CAAATTCCAG CAACTTCTAA AACAAAATTC 
CTTAAAATAG CATCTTCTGA GGAAGACTCT AAAAGTCATG ATTCAGATAA TGGACATAGC 
AGAACTGCTG GCCCATCAAC ATCTTTGCTG AAACTCAAGG CAGAAAGCAT TTCAGAGGAA 
GAGGAGAAAG CAAATTCTGA AACAAGAAAA TACAGCAGTA GGAAATACAG CACAGGTCGC 
AAGAATGCTC CTTTCCTTAA AAAAGCAAAG CTCTTCAGTG ATTCAGAGGA CTCTGAGTCT 
GACAAAGAAG ATGGACAAGA CAGATGTCAT AAGATGGAAA TGAACCCGAG TTCAGGGAAT 
GTGGAATGTG AACCTGCTGC CAGATCCCAG TGTTTTTCAG ATCATGTATC TGAAACTGAT 
TTGGATTCAG ATGATGACAA GAAAGCAAAA GAAAAACCAA ATAGTTTTAT AAAAGATTCT 
GCATCACAAA ATCATGGACA GAGCAGGAAA GCTGCCAGGA AAAGGGTCTG TTCCAGTGAC 
TCAGACAGCA ATTCGAAGGT GGTTAAGAAA GCATCCAGAG CCAGACCAGG CCTTCTGAGA 
GTGCCTCGGA GGTGTGCCAC CACTGCTGCC AGTCGGATCA AGCTGATGAG TGATGAGGAA 
GATGGCCGTG TAGACCACGT GTGCACCAGG CGCAAAAATG CAAGGAAAAA GCCCCTCCAT 
ATTGCATGTG CCCCAGCTCA GAAGATAGAC AGCAATTCTG AGGGGGATGT AAACTGTGAA 
GTGCCAAACG AACAGAGTGG CTGTGAAGGG CAGCCACTTG ATGCCGACTC AGAAGGTAGT 
GCAAAAAGTA TCAGTCAGTC TTTAAATGAA GACTCAGACT CTGAAGGTGT GTTGAATTCA 
GGACACAAGA ACAGGCATAC CAGGAGTCTC AAAACCAGTG TTGCATCTTC TAAAACAAAG 
AATGCCTTGA TTGTGTCTTT GGAGGAAGAT TCAAAAAGCC ATATTCCACG GGGTGAGATA 
GGTAGAAAAT GTTCTTCTGA GTCAACTTTG GTGCACAAAG CTACTGCAGA GAATAACTTT 
GAAGAGGAAC TGAACTATGG GCTACGTAGG TGGAACGGCA GGAGGCTCCG GACCTATGGG 
AAGGCTCCTT TCAGTAAGAC AAAAGTGGTG CGGGATTCGC AGGAAGCTGC CGAGACAGAA 
GTAAAAAGGA AGAGACTGCA AACTGAATTG GAAAATGTGA AAGCTTCCGA AACAACAGGG 
AGTTCAAAGT GTGGACCAGA CACTAGCCCC AAATCTGACT CAGATTTGGG ATCTGCCACT 
GAATCAGATG CTGACTGTAC AGATGATTCA AAAACCAAAA AGAGGAAAAC GAAAGGGAAA 
GCAAAAGTAG TTAGAAAAGA ATCTGTTCCT AGAGACAGAG AACCCAATAC AAAAATGAGA 
ACATGTGTGC ATAATCAGAA GGATGCAGTG CCAATGCCTA GTGAGACTCT GAAAGCAAAA 
ATGGTACCTG AGAAAGTTCC CCGCAGATGT GCTACTGTTG CTGCAAATAA AATAAAGATA 
ATGAGTAATC TAAAGGAAAC CATTTCAGGA CCAGAAAATG TGTGGATTAG GAAGAGCAGC 
AGAAAACTGC CCCATCGAAA TGCTTCTGCT GCGGCTAAGA AAAAATTACT CAATGTTTAT 
AAAGAGGATG ATACAACCAT AAATTCTGAA AGTGAAAAGG AGCTAGAAGA TATTAATAGA 
AAAATGCCCC TTTTAAGGCG TTTCAGATCC TGGAAAGAAA AAGCACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847118.1
CDS	151..6861
Translation	MLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGAMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSLEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLLGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLVPQLGYVATSDGEVIEQIISLQTNDNGERSPERSVLDGMIRQLQQQQDQRMGAGQEAVPSGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQMATERDLQAWKRRVVVPEVPLGIFRKLEDFRIEKGEQERNLYIIGRKRKTLQFSQKSDSVTFMSQSRQRTCRRKYPNYGRRNLGRLELSSGNESSSSIRHETSCDQSEGSCSSEEEEWKSDRRSESDSESSSDSSSRYSDWTADAGIHLQPPLRTSSRRRITRFCSSSEDEMSAENLSPPKRRRKRKKENKPKKENLRRMTQAELADVEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLRQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTSDELEKLLYKPQDGEWGQKSRDEECERIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQDCINISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDWKKRSIKTTNYVENNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLEAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFSEKLRRSQRFKRRQNCNRVNQANKSIRNTKQKRLKSQTKVISEFVGSPIQPTSSRATYSASHKTTASVSSGVTSGDSSDSAASSERMRRNRPVTLTNGSTLSESEMEDSLGTSSSSSASNSSEESKESPRARDSSSRSGLARSSNLRVTRTRAAQRKTGPVSLENGCGRKATRKRVYLSDSDNNSLATGESVKVRAGNNRKVLRKCAAVAANKIKLMSDVEENSTSESVCSGRKLPHRNASAVARKKLLHNSEDDQSLKSELEEEELKDQLSPLSNSLAAQNTENGDSESESDLRVARKNWHANGYKSQIPATSKTKFLKIASSEEDSKSHDSDNGHSRTAGPSTSLLKLKAESISEEEEKANSETRKYSSRKYSTGRKNAPFLKKAKLFSDSEDSESDKEDGQDRCHKMEMNPSSGNVECEPAARSQCFSDHVSETDLDSDDDKKAKEKPNSFIKDSASQNHGQSRKAARKRVCSSDSDSNSKVVKKASRARPGLLRVPRRCATTAASRIKLMSDEEDGRVDHVCTRRKNARKKPLHIACAPAQKIDSNSEGDVNCEVPNEQSGCEGQPLDADSEGSAKSISQSLNEDSDSEGVLNSGHKNRHTRSLKTSVASSKTKNALIVSLEEDSKSHIPRGEIGRKCSSESTLVHKATAENNFEEELNYGLRRWNGRRLRTYGKAPFSKTKVVRDSQEAAETEVKRKRLQTELENVKASETTGSSKCGPDTSPKSDSDLGSATESDADCTDDSKTKKRKTKGKAKVVRKESVPRDREPNTKMRTCVHNQKDAVPMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMPLLRRFRSWKEKAQ

Target ORF information:

RefSeq Version	XM_024991350.1
Organism	Bos taurus(cattle)
Definition	Bos taurus bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991350.1

ORF Insert Sequence:

ATGCTGGATA AAGAGATCCC ACCCAGCATT TCAAGAGTCA CTTCTTTACT TGGGGCAGGA 
AGGCAGTCTT TGCTACGTAC AGCAAAAGAC TGCAGGCACA CAGTTTGGAA GGGCTCTGCC 
TTTGCTGCTC TTCATAGAGG AAGACCTCCT GAAATGCCGG TGAATTATGG TTCCCCACCA 
AATCTTGTGG AGATCCATCG AGGAAAACAG CTCACAGGGT GTTCCACTTT TAGCACAGCA 
TTTCCAGGAG CTATGTATCA GCACATAAAA ATGCACAGGA GGATTCTCGG ACATCTGTCT 
GCTGTTTACT GTGTAGCATT TGATAGGACA GGACATAGAA TCTTTACAGG TTCAGATGAT 
TGTTTGGTGA AGATTTGGTC CACACACAAT GGCCGCCTAT TGTCCACATT AAGAGGTCAT 
TCTGCAGAAA TTTCAGATAT GGCAGTAAAC TATGAGAATA CAATGATTGC TGCAGGGAGC 
TGTGATAAAA TAATTAGAGT GTGGTGCCTG AGAACCTGTG CTCCAGTTGC TGTGCTCCAA 
GGACACACGG GGTCAATTAC ATCTTTACAG TTTAGCCCCA TGGCCAAAGG CTCGCAAAGA 
TACATGGTCT CCACTGGTGC TGATGGGACT GTTTGTTTTT GGCAGTGGGA TTTAGAATCC 
CTGAAATTCA GTCCACGTCC CCTGAAGTTC ACTGAAAAGC CTAGACCAGG TGTTCAAATG 
CTGTGTTCTT CATTTAGTGT TGGTGGTATG TTTCTAGCCA CAGGTAGTAC TGATCATGTA 
ATCAGAATGT ATTTTTTGGG TTTTGAAGCA CCTGAAAAAA TCGCAGAACT TGAAAGTCAC 
ACTGATAAAG TTGATAGTAT CCAATTTTGT AACAATGGTG ATCGGTTCTT AAGTGGTAGC 
AGAGATGGAA CAGCACGAAT TTGGAGATTT GAACAGTTAG AATGGAGAAG CATCTTACTA 
GATATGGCCA CTAGAATCTC AGGAGATTTA TCTTTGGAAG AGGAAAGGTT TATGAAACCC 
AAAGTAACAA TGATAGCTTG GAATCAGAAT GATAGCATTG TTGTCACAGC TGTGAATGAT 
CATGTCCTCA AAGTGTGGAA TTCTTACACT GGACAGCTGC TTCATAACCT GTTGGGACAT 
GCTGATGAAG TATTTGTTTT GGAGACACAT CCCTTTGATT CCAGAATTAT GTTATCTGCA 
GGACATGACG GCAGCATATT CATATGGGAT ATCACAAAAG GCACCAAGAT GAAACATTAT 
TTCAATATGA TTGAAGGACA AGGACATGGA GCTGTCTTTG ATTGTAAGTT TTCACAGGAT 
GGACAGCATT TTGCCTGTAC AGATTCTCAT GGTCACCTTC TGATATTTGG TTTTGGATGC 
AGCAAGCCAT ATGAAAAGAT TCCTGACCAG ATGTTCTTCC ATACTGACTA TCGACCGCTG 
ATTAGGGATT CTAATAATTA CGTGTTAGAC GAGCAGACTC AGCAGGCCCC TCACCTTATG 
CCTCCACCGT TCTTGGTAGA TGTAGATGGA AACCCTCATC CAACTAAGTA TCAGCGATTA 
GTGCCTGGCC GAGAAAATTC TGCAGATGAA CATTTGGTTC CACAACTAGG CTATGTGGCA 
ACAAGTGATG GAGAGGTAAT AGAACAAATT ATAAGCCTGC AAACCAATGA TAATGGTGAA 
CGAAGCCCAG AGCGCAGTGT CCTGGATGGA ATGATAAGAC AGTTGCAGCA GCAGCAAGAT 
CAGAGAATGG GAGCAGGTCA GGAGGCTGTT CCAAGTGGAC TTTCAAATGG GGAAGAAACA 
CCCCGAAGAG GTTTTAGAAG ACTAAGCTTA GACATCCAGT CTCCTCCAAA TATTGGTCTG 
CGTCGTAGTG GACAAGTGGA AGGTGTCCGT CAGATGCACC AGAATGCTCC GCGCAGTCAG 
ATGGCTACGG AGCGTGACCT GCAGGCTTGG AAGCGAAGAG TGGTTGTCCC AGAGGTACCA 
CTAGGCATAT TTAGGAAGTT GGAAGACTTC AGAATAGAGA AAGGTGAACA AGAAAGAAAT 
CTTTATATAA TTGGAAGAAA GAGGAAGACT CTCCAGTTCT CACAAAAGTC TGATTCGGTG 
ACTTTTATGT CACAGTCTAG ACAGAGGACA TGTAGGCGTA AATATCCAAA TTATGGTAGA 
AGAAATCTTG GACGGCTTGA GTTGTCTTCT GGAAATGAAT CTTCAAGCTC CATAAGACAT 
GAGACTTCTT GTGATCAAAG TGAAGGTTCT TGTTCTTCTG AAGAAGAAGA ATGGAAAAGT 
GATAGAAGGA GTGAAAGTGA CAGTGAAAGT TCAAGTGACT CTTCCTCTAG GTATTCTGAT 
TGGACAGCTG ACGCAGGCAT CCACCTGCAG CCTCCTTTAC GAACATCATC TCGTCGACGA 
ATTACTCGAT TTTGCAGCAG TTCAGAGGAT GAAATGTCTG CTGAGAATTT ATCTCCTCCA 
AAAAGGAGAC GAAAGAGAAA GAAAGAAAAT AAGCCTAAAA AAGAGAATTT GCGGAGGATG 
ACTCAGGCAG AGCTTGCAGA CGTGGAACAT TTATATGAAT TTCATCCTCC AGTTTGGATA 
ACTGACACCA CACTTCGAAA ATCTCCTTTT GTTCCTCAAA TGGGTGATGA GGTAATATAT 
TTTCGACAGG GTCATGAAGC TTATATTGAG GCTGTCAGAA GAAATAACAT TTATGAACTG 
AACCCTAACA AGGAGCCATG GAGAAAAATG GATCTTAGGG ATCAAGAATT GGTCAAAATA 
GTTGGAATAC GATATGAGGT TGGACCCCCG ACACTTTGTT GCCTCAAACT AGCATTTATA 
GATCCTGCAA CTGGAAAACT TATGGACAAA TCTTTCTCTA TTAGATACCA TGACATGCCA 
GATGTTATTG ACTTTCTTGT CTTGCGTCAG TTTTATGATG AAGCAAGACA GAGGAATTGG 
CAGTCTTGTG ACAGGTTCCG TTCCATCATT GATGATGCTT GGTGGTTTGG AACGGTGCTA 
CGTCAAGAGC CGTATCAGCC ACAGTACCCT GATAGTCATT TCCAGTGTTA CATTGTGAGG 
TGGGACAATA CTGAAATTGA AAAACTTAGC CCATGGGATA TGGAACCAAT ACCTGACAAT 
GTTGATCCAC CTGAAGAATT AGGCGCTAGT ATTTCTGTCA CTTCAGATGA GCTAGAGAAA 
TTGCTCTACA AACCCCAAGA TGGTGAATGG GGTCAGAAAT CAAGAGATGA AGAATGTGAA 
CGAATTATTA GTGGTATAGA TCAACTTTTG AATCTTGATA TTGCAGCAGC TTTTGCAGGT 
CCGGTTGATC TGTGTACATA TCCGAAGTAC TGTACTGTAG TGGCTTACCC AACTGATCTG 
TACACCATTC GAATGAGACT TGTGAATCGA TTTTACAGGA GGCTATCTGC GTTAGTTTGG 
GAAGTCAGAT ACATAGAACA CAATGCCAGA ACATTTAATG AACCTGAGAG TGTAATTGCA 
AGATCAGCTA AGAAGATAAC AGACCAGCTT TTAAAATTTA TCAAGAATCA AGACTGTATA 
AATATCTCAG AACTTTCTAA CACTTCTGAA AATGATGAGC AAAATGCCGA GGATTTGGAT 
GATAGTGATC TTCCTAAAAC ATCTTCTGGA AGAAGGAGAG TTCATGATTG GAAAAAAAGG 
AGCATCAAAA CAACCAATTA TGTTGAAAAC AACTGGAAGA AACAGTGTAA GGAACTAGTG 
AACTTAATTT TTCAATGTGA AGATTCTGAA CCATTTAGAC AACCTGTTGA TTTGGTTGAA 
TATCCAGATT ACCGAGACAT TATAGATACT CCAATGGATT TTGGAACAGT AAGGGAAACT 
TTAGAAGCGG GAAACTATGA CAGCCCTTTG GAATTTTGCA AAGACATCCG TTTGATATTT 
AGCAATGCAA AAGCATATAC ACCAAACAAA AGATCAAAGA TTTACAGTAT GACATTGAGA 
TTGTCTGCCT TATTTGAAGA AAAAATGAAG AAAATCTCCT CTGATTTTAA AATTGGTCAA 
AAATTCAGTG AAAAACTTCG AAGAAGCCAG AGGTTTAAGA GACGACAAAA TTGTAACCGT 
GTCAATCAGG CTAACAAAAG TATCAGAAAT ACCAAGCAGA AGCGTTTAAA ATCTCAGACA 
AAAGTAATTT CTGAGTTCGT AGGTTCTCCT ATCCAGCCTA CCTCAAGTAG GGCAACTTAC 
TCCGCAAGCC ACAAGACAAC TGCTAGTGTC TCTTCAGGTG TTACTTCTGG TGACTCTTCA 
GATTCAGCAG CATCATCAGA AAGAATGAGA AGAAATAGAC CTGTCACTCT GACAAATGGT 
TCTACATTAT CTGAAAGTGA AATGGAAGAT TCTTTAGGTA CCTCTTCATC ATCTTCAGCT 
TCAAATAGTT CTGAGGAAAG CAAGGAGAGT CCAAGAGCTC GAGACTCCTC CTCCCGTAGT 
GGGCTAGCCA GGAGCAGCAA CCTCAGGGTG ACCAGGACCA GAGCTGCACA GAGAAAAACT 
GGTCCAGTTT CTTTAGAAAA TGGATGTGGC AGAAAAGCCA CTAGGAAGAG AGTCTATTTA 
AGTGACTCAG ATAACAATTC TTTGGCGACT GGTGAAAGTG TAAAAGTGAG AGCTGGAAAT 
AACCGGAAAG TGCTACGAAA ATGTGCTGCT GTGGCTGCCA ATAAAATCAA GTTAATGAGT 
GATGTAGAGG AGAATTCTAC CTCTGAAAGT GTCTGTTCTG GCCGTAAGCT GCCCCACCGC 
AATGCTTCTG CTGTAGCTAG AAAAAAATTA TTACATAATT CTGAGGATGA CCAGAGCTTG 
AAGTCAGAAC TTGAAGAAGA GGAGCTAAAA GATCAACTAT CACCATTGTC CAACTCTCTT 
GCTGCCCAGA ATACTGAAAA TGGAGATTCA GAGTCAGAAA GTGATTTACG GGTAGCCCGG 
AAAAATTGGC ATGCTAATGG TTACAAGTCC CAAATTCCAG CAACTTCTAA AACAAAATTC 
CTTAAAATAG CATCTTCTGA GGAAGACTCT AAAAGTCATG ATTCAGATAA TGGACATAGC 
AGAACTGCTG GCCCATCAAC ATCTTTGCTG AAACTCAAGG CAGAAAGCAT TTCAGAGGAA 
GAGGAGAAAG CAAATTCTGA AACAAGAAAA TACAGCAGTA GGAAATACAG CACAGGTCGC 
AAGAATGCTC CTTTCCTTAA AAAAGCAAAG CTCTTCAGTG ATTCAGAGGA CTCTGAGTCT 
GACAAAGAAG ATGGACAAGA CAGATGTCAT AAGATGGAAA TGAACCCGAG TTCAGGGAAT 
GTGGAATGTG AACCTGCTGC CAGATCCCAG TGTTTTTCAG ATCATGTATC TGAAACTGAT 
TTGGATTCAG ATGATGACAA GAAAGCAAAA GAAAAACCAA ATAGTTTTAT AAAAGATTCT 
GCATCACAAA ATCATGGACA GAGCAGGAAA GCTGCCAGGA AAAGGGTCTG TTCCAGTGAC 
TCAGACAGCA ATTCGAAGGT GGTTAAGAAA GCATCCAGAG CCAGACCAGG CCTTCTGAGA 
GTGCCTCGGA GGTGTGCCAC CACTGCTGCC AGTCGGATCA AGCTGATGAG TGATGAGGAA 
GATGGCCGTG TAGACCACGT GTGCACCAGG CGCAAAAATG CAAGGAAAAA GCCCCTCCAT 
ATTGCATGTG CCCCAGCTCA GAAGATAGAC AGCAATTCTG AGGGGGATGT AAACTGTGAA 
GTGCCAAACG AACAGAGTGG CTGTGAAGGG CAGCCACTTG ATGCCGACTC AGAAGGTAGT 
GCAAAAAGTA TCAGTCAGTC TTTAAATGAA GACTCAGACT CTGAAGGTGT GTTGAATTCA 
GGACACAAGA ACAGGCATAC CAGGAGTCTC AAAACCAGTG TTGCATCTTC TAAAACAAAG 
AATGCCTTGA TTGTGTCTTT GGAGGAAGAT TCAAAAAGCC ATATTCCACG GGGTGAGATA 
GGTAGAAAAT GTTCTTCTGA GTCAACTTTG GTGCACAAAG CTACTGCAGA GAATAACTTT 
GAAGAGGAAC TGAACTATGG GCTACGTAGG TGGAACGGCA GGAGGCTCCG GACCTATGGG 
AAGGCTCCTT TCAGTAAGAC AAAAGTGGTG CGGGATTCGC AGGAAGCTGC CGAGACAGAA 
GTAAAAAGGA AGAGACTGCA AACTGAATTG GAAAATGTGA AAGCTTCCGA AACAACAGGG 
AGTTCAAAGT GTGGACCAGA CACTAGCCCC AAATCTGACT CAGATTTGGG ATCTGCCACT 
GAATCAGATG CTGACTGTAC AGATGATTCA AAAACCAAAA AGAGGAAAAC GAAAGGGAAA 
GCAAAAGTAG TTAGAAAAGA ATCTGTTCCT AGAGACAGAG AACCCAATAC AAAAATGAGA 
ACATGTGTGC ATAATCAGAA GGATGCAGTG CCAATGCCTA GTGAGACTCT GAAAGCAAAA 
ATGGTACCTG AGAAAGTTCC CCGCAGATGT GCTACTGTTG CTGCAAATAA AATAAAGATA 
ATGAGTAATC TAAAGGAAAC CATTTCAGGA CCAGAAAATG TGTGGATTAG GAAGAGCAGC 
AGAAAACTGC CCCATCGAAA TGCTTCTGCT GCGGCTAAGA AAAAATTACT CAATGTTTAT 
AAAGAGGATG ATACAACCAT AAATTCTGAA AGTGAAAAGG AGCTAGAAGA TATTAATAGA 
AAAATGCCCC TTTTAAGGCG TTTCAGATCC TGGAAAGAAA AAGCACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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BRWD1 cDNA ORF clone, Bos taurus(Cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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