ANKLE2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKLE2
Entrez Gene ID	540159
Full Name	ankyrin repeat and LEM domain containing 2
General protein information	Preferred Names ankyrin repeat and LEM domain containing 2 Names ankyrin repeat and LEM domain-containing protein 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_591880.8	XP_591880.5	ankyrin repeat and LEM domain-containing protein 2
XM_002694448.4	XP_002694494.2	ankyrin repeat and LEM domain-containing protein 2
XM_002694448.5	XP_002694494.3	ankyrin repeat and LEM domain-containing protein 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ankle2	NP_001041366.1
Homo sapiens (human)	ANKLE2	NP_055929.1
Bos taurus (cattle)	ANKLE2	XP_002694494.2
Macaca mulatta (Rhesus monkey)	ANKLE2	XP_001087028.1
Danio rerio (zebrafish)	ankle2	NP_001119867.1
Xenopus tropicalis (tropical clawed frog)	ankle2	XP_004910629.1
Canis lupus familiaris (dog)	ANKLE2	XP_852228.2
Pan troglodytes (chimpanzee)	ANKLE2	XP_001152324.2
Mus musculus (house mouse)	Ankle2	NP_001240743.1
Gallus gallus (chicken)	ANKLE2	XP_415084.4

Gene Ontology

The following ANKLE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKLE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa08968	XM_591880.8 Latest version!	Bos taurus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa08968	XM_002694448.4 Latest version!	Bos taurus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa224548	XM_002694448.5 Latest version!	Bos taurus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKLE2 ( XM_591880.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_591880.5 Bos taurus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

Price & Availability↑

CloneID	OBa08968
Clone ID Related Accession (Same CDS sequence)	XM_591880.8 , XM_002694448.4
Accession Version	XM_591880.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_591880.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGC CGCGGCTGGC GGCGACTGAG TGGGCGGCGT TGGCCTGGGA GTTGCTGGGC 
GCCTCGGTGC TGCTGATCGC GGTGCGCTGG CTTGTGCGGC GGTTGGACAG GCGGCCGCGG 
GGCCTGGGTC AGAGCGGTCC ACCCGACCCG CCGTCCCGCG CGGTCGCGGG CCCAGCCCCG 
GATTCAGGTA AAATGACAAT GGATACCATC TTGGCGCGAT TGAAGCTCCT GAATCCAGAT 
GAGCTTAGAG AAGAGATTGT CAAAGCTGGA TTGAAATGTG GACCCATTAC ATCAACAACC 
AGGTTCATTT TTGAGAAAAA ACTGGCTCAG GCTTTATTAG AGCGTGGTAC ACTGCCTTCA 
CACCTGTCCG ACCCTGGTGC CGCAGATGCC CCATCCCTGG GCCAGGACAC ACAAAGGATT 
GTGAAGTCTG CAGTAGGGAG CCCAGCTGAG CAGGTCAGCT TTTCTGAAGA CAGAGACTTT 
GGTTATAGTG TGGGCTTGAA TCCTCCAGAG GAAGACGCCG TGACATCTAA GACCTGTTCA 
GCGCCCTTCA GTGCCTCAGG CGGCATCAAC AGCCAGAAAG CCGTGCTGAG GGCCTCTGCA 
GAGGTGCCTC TCTACTACGG GGTGTGCCCA GCGGCCTTCG AGGATACTCC AGCAAGAAAT 
GAAAGGATTC ACGTTTATGA AGATAAAAAG GAAGCCTTAC AAGCTGTCAA GATGAACAAA 
GGATCCCGAT TTAAAGCTTT TACAAACCGA GAAGATGCTG AGAAATTTGC CAGAGGAAGT 
TGTGATTATT TCCCTTCTCC AAGCAAAACC TCTTTACCAC TTTCTCCTGT GAAAACGGCA 
CCACTCTTTA GCAATGACGG GTTGTACTTG TCTGAGTCAG AAACAGCCAG CAAAGAGCGA 
GCAAACAGCT ATAAAAATCC ACGCACACAA GACCTCACTG CCAAACTTCG GAAAGCTGTG 
GAGAAGGGAG ACGAAGACAC CTTTTCCGAC CTCATCTGGA GTAATCCCCG GTATCTGATT 
GGTTCAGGGG ACAACCCAAC CATCGTACAG GAAGGGTGTC GGTACAATGT CATGCATGTG 
GCTGCCAAAG AGAACCAGGC TGCTGTGTGC CAGCTCACCC TAGAGACGCT GGAGAACCCC 
GAGTTCATGC GGCTCATGTA CCCAGATGAC GAGCCGCACA TGCTGGAGAA GCGCATTTGC 
TATGTGGTGG ACCTGTACCT CAACACACCG GACAGAGCGG GCTATGACAC ACCATTGCAT 
TTTGCTTGTA AGTTTGGAAA TGTAGATGTG GTCAATGTGC TTTCTTCACA CCCTTTGATT 
GTAAAAAACC CAAGGAATAA ATATGATAAA ACACCTGAAG ACGTTATTTG TGAAAGAAGC 
AAAAACAAAT CTGTAGAACT GAAGGAGCGG ATTCGAGACT ACTTAAAGGG TCACTACTAT 
GTGCCCCTCC TGAGGGCAGA GGACACGTCT TCTCCTGTGA TCGGGGAGCT GTGGTCTTCA 
GACCAGACAG GCGAGGTCTC TCACATTGGC CACACTGGAG GCTTCCCCAA AGACCCTGTT 
CTAACCCTTC GAGCCTTCGC AGGGCCCCTG AGTCCATCCA AGGCAGAAGA TTTTCGCCGA 
CTCTGGAAAA CCCCGCCTCG AGAGAAAGCG GGCTTCTTCC ACAGTGTCAG GAAATCTGAT 
CCAGAAAGAG GCATTGAGAG AGTGGGAAGG GAGCTGGCTC ATGAACTGGG GTATCCCTGG 
GTCGAATACT GGGACTTTCT GGGCTGTTTT GTTGATCTGT CTTCCCAGGC AGGCCTGCAA 
AAGCTGGAAG AATACCTTAC TCAGCAGGAA GTAGGCAAAA AGCACCTACA AGACATGGGA 
GAAAATGCAG CGTGTCGCCA GGAGGATGCC TCAGCCCTAG GCCGTCAGAG GAAGTGCAGC 
AATTCCATCT CTGTGAGGGC GTTTCTAGAT GAAGACGATG ACATGAGCTT GGAAGAGATA 
AAAAACCGGC AAAACACAGC TCGAAACAAC ACCCCGCCCA GGGCAGCTGC TTTTGGAGAC 
TTGGGGTGTG ACATCCTCCC CTTGGAGCAG ACAGAACTTA TCGAAGCCTC AGCCCCGACC 
AGTCCCCACA GCAGCAGAAA TGGGTTTTGC AGCCCTCTAA GGGATGGCAA GACCCTGGGT 
GGGAAGAGGC CCAAGGCCCA GAGTGGGGAG GAAGACCTCC GCTCACCCAT CTCCGGCTTG 
ACTGTTGAGT TTGATAAACT GAATTTGCAG AATCTAGAAA GTAGCTTTTC TAAGACACCA 
AATCAAAGAG TGAAAACTAG AGATAAGAAG GTCCTGACAT CAAGAGTGAA CGCGGGAGGA 
AGTGACGTGT TAGAACCTCT TGCTGCTGAT AGACTTGGAA ATAACCAGGG AAGGACAGAA 
AATGAGATGT CTGCTGGAAT TGCTAACATG TGCCTGAATC CAGACAGTTC TGTGCACGAG 
CCCCGCTATG GCAATGGCTC GGAGCCCTCG GAGATTCCCG CCTCAAAGGA GCCTGTCCAG 
AGGCTCTTCC TTTTTGGAGA AGAGCCGTCA AAACTGGATC GGGATGTTCT GGCAGCCCTG 
GAGTGCACGG ATGTGGACCC TCGCCAGTAC CCGGCCGTAC ACAGGTGGAG GAGCGCTGTC 
CTTTGCTACT CGCCTTCGGA CAGACAGAGC TGGTCCAGCC CCTCGCTGAA AGGGAAGTTG 
AAGTCTCAGC TCCTGGACGT CAGTAGCCCT CCTAGCGGCA GCCCTGGGAG AAGCAGCCCT 
GCAGGCAGCA GCCCTGGGAA GCCTGGCTCC ACACCTCACG CCTCCGGCCT GGGCAGCCCC 
GGGCGCTACA GTCCCGCCAA CGGGGGCCAC CTCCGCCGGA TGGCGCGCCT GGCCCAGCTT 
GCCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_591880.5
CDS	42..2933
Translation	MLWPRLAATEWAALAWELLGASVLLIAVRWLVRRLDRRPRGLGQSGPPDPPSRAVAGPAPDSGKMTMDTILARLKLLNPDELREEIVKAGLKCGPITSTTRFIFEKKLAQALLERGTLPSHLSDPGAADAPSLGQDTQRIVKSAVGSPAEQVSFSEDRDFGYSVGLNPPEEDAVTSKTCSAPFSASGGINSQKAVLRASAEVPLYYGVCPAAFEDTPARNERIHVYEDKKEALQAVKMNKGSRFKAFTNREDAEKFARGSCDYFPSPSKTSLPLSPVKTAPLFSNDGLYLSESETASKERANSYKNPRTQDLTAKLRKAVEKGDEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQAAVCQLTLETLENPEFMRLMYPDDEPHMLEKRICYVVDLYLNTPDRAGYDTPLHFACKFGNVDVVNVLSSHPLIVKNPRNKYDKTPEDVICERSKNKSVELKERIRDYLKGHYYVPLLRAEDTSSPVIGELWSSDQTGEVSHIGHTGGFPKDPVLTLRAFAGPLSPSKAEDFRRLWKTPPREKAGFFHSVRKSDPERGIERVGRELAHELGYPWVEYWDFLGCFVDLSSQAGLQKLEEYLTQQEVGKKHLQDMGENAACRQEDASALGRQRKCSNSISVRAFLDEDDDMSLEEIKNRQNTARNNTPPRAAAFGDLGCDILPLEQTELIEASAPTSPHSSRNGFCSPLRDGKTLGGKRPKAQSGEEDLRSPISGLTVEFDKLNLQNLESSFSKTPNQRVKTRDKKVLTSRVNAGGSDVLEPLAADRLGNNQGRTENEMSAGIANMCLNPDSSVHEPRYGNGSEPSEIPASKEPVQRLFLFGEEPSKLDRDVLAALECTDVDPRQYPAVHRWRSAVLCYSPSDRQSWSSPSLKGKLKSQLLDVSSPPSGSPGRSSPAGSSPGKPGSTPHASGLGSPGRYSPANGGHLRRMARLAQLATL

Target ORF information:

RefSeq Version	XM_591880.8
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_591880.8

ORF Insert Sequence:

ATGCTGTGGC CGCGGCTGGC GGCGACTGAG TGGGCGGCGT TGGCCTGGGA GTTGCTGGGC 
GCCTCGGTGC TGCTGATCGC GGTGCGCTGG CTTGTGCGGC GGTTGGACAG GCGGCCGCGG 
GGCCTGGGTC AGAGCGGTCC ACCCGACCCG CCGTCCCGCG CGGTCGCGGG CCCAGCCCCG 
GATTCAGGTA AAATGACAAT GGATACCATC TTGGCGCGAT TGAAGCTCCT GAATCCAGAT 
GAGCTTAGAG AAGAGATTGT CAAAGCTGGA TTGAAATGTG GACCCATTAC ATCAACAACC 
AGGTTCATTT TTGAGAAAAA ACTGGCTCAG GCTTTATTAG AGCGTGGTAC ACTGCCTTCA 
CACCTGTCCG ACCCTGGTGC CGCAGATGCC CCATCCCTGG GCCAGGACAC ACAAAGGATT 
GTGAAGTCTG CAGTAGGGAG CCCAGCTGAG CAGGTCAGCT TTTCTGAAGA CAGAGACTTT 
GGTTATAGTG TGGGCTTGAA TCCTCCAGAG GAAGACGCCG TGACATCTAA GACCTGTTCA 
GCGCCCTTCA GTGCCTCAGG CGGCATCAAC AGCCAGAAAG CCGTGCTGAG GGCCTCTGCA 
GAGGTGCCTC TCTACTACGG GGTGTGCCCA GCGGCCTTCG AGGATACTCC AGCAAGAAAT 
GAAAGGATTC ACGTTTATGA AGATAAAAAG GAAGCCTTAC AAGCTGTCAA GATGAACAAA 
GGATCCCGAT TTAAAGCTTT TACAAACCGA GAAGATGCTG AGAAATTTGC CAGAGGAAGT 
TGTGATTATT TCCCTTCTCC AAGCAAAACC TCTTTACCAC TTTCTCCTGT GAAAACGGCA 
CCACTCTTTA GCAATGACGG GTTGTACTTG TCTGAGTCAG AAACAGCCAG CAAAGAGCGA 
GCAAACAGCT ATAAAAATCC ACGCACACAA GACCTCACTG CCAAACTTCG GAAAGCTGTG 
GAGAAGGGAG ACGAAGACAC CTTTTCCGAC CTCATCTGGA GTAATCCCCG GTATCTGATT 
GGTTCAGGGG ACAACCCAAC CATCGTACAG GAAGGGTGTC GGTACAATGT CATGCATGTG 
GCTGCCAAAG AGAACCAGGC TGCTGTGTGC CAGCTCACCC TAGAGACGCT GGAGAACCCC 
GAGTTCATGC GGCTCATGTA CCCAGATGAC GAGCCGCACA TGCTGGAGAA GCGCATTTGC 
TATGTGGTGG ACCTGTACCT CAACACACCG GACAGAGCGG GCTATGACAC ACCATTGCAT 
TTTGCTTGTA AGTTTGGAAA TGTAGATGTG GTCAATGTGC TTTCTTCACA CCCTTTGATT 
GTAAAAAACC CAAGGAATAA ATATGATAAA ACACCTGAAG ACGTTATTTG TGAAAGAAGC 
AAAAACAAAT CTGTAGAACT GAAGGAGCGG ATTCGAGACT ACTTAAAGGG TCACTACTAT 
GTGCCCCTCC TGAGGGCAGA GGACACGTCT TCTCCTGTGA TCGGGGAGCT GTGGTCTTCA 
GACCAGACAG GCGAGGTCTC TCACATTGGC CACACTGGAG GCTTCCCCAA AGACCCTGTT 
CTAACCCTTC GAGCCTTCGC AGGGCCCCTG AGTCCATCCA AGGCAGAAGA TTTTCGCCGA 
CTCTGGAAAA CCCCGCCTCG AGAGAAAGCG GGCTTCTTCC ACAGTGTCAG GAAATCTGAT 
CCAGAAAGAG GCATTGAGAG AGTGGGAAGG GAGCTGGCTC ATGAACTGGG GTATCCCTGG 
GTCGAATACT GGGACTTTCT GGGCTGTTTT GTTGATCTGT CTTCCCAGGC AGGCCTGCAA 
AAGCTGGAAG AATACCTTAC TCAGCAGGAA GTAGGCAAAA AGCACCTACA AGACATGGGA 
GAAAATGCAG CGTGTCGCCA GGAGGATGCC TCAGCCCTAG GCCGTCAGAG GAAGTGCAGC 
AATTCCATCT CTGTGAGGGC GTTTCTAGAT GAAGACGATG ACATGAGCTT GGAAGAGATA 
AAAAACCGGC AAAACACAGC TCGAAACAAC ACCCCGCCCA GGGCAGCTGC TTTTGGAGAC 
TTGGGGTGTG ACATCCTCCC CTTGGAGCAG ACAGAACTTA TCGAAGCCTC AGCCCCGACC 
AGTCCCCACA GCAGCAGAAA TGGGTTTTGC AGCCCTCTAA GGGATGGCAA GACCCTGGGT 
GGGAAGAGGC CCAAGGCCCA GAGTGGGGAG GAAGACCTCC GCTCACCCAT CTCCGGCTTG 
ACTGTTGAGT TTGATAAACT GAATTTGCAG AATCTAGAAA GTAGCTTTTC TAAGACACCA 
AATCAAAGAG TGAAAACTAG AGATAAGAAG GTCCTGACAT CAAGAGTGAA CGCGGGAGGA 
AGTGACGTGT TAGAACCTCT TGCTGCTGAT AGACTTGGAA ATAACCAGGG AAGGACAGAA 
AATGAGATGT CTGCTGGAAT TGCTAACATG TGCCTGAATC CAGACAGTTC TGTGCACGAG 
CCCCGCTATG GCAATGGCTC GGAGCCCTCG GAGATTCCCG CCTCAAAGGA GCCTGTCCAG 
AGGCTCTTCC TTTTTGGAGA AGAGCCGTCA AAACTGGATC GGGATGTTCT GGCAGCCCTG 
GAGTGCACGG ATGTGGACCC TCGCCAGTAC CCGGCCGTAC ACAGGTGGAG GAGCGCTGTC 
CTTTGCTACT CGCCTTCGGA CAGACAGAGC TGGTCCAGCC CCTCGCTGAA AGGGAAGTTG 
AAGTCTCAGC TCCTGGACGT CAGTAGCCCT CCTAGCGGCA GCCCTGGGAG AAGCAGCCCT 
GCAGGCAGCA GCCCTGGGAA GCCTGGCTCC ACACCTCACG CCTCCGGCCT GGGCAGCCCC 
GGGCGCTACA GTCCCGCCAA CGGGGGCCAC CTCCGCCGGA TGGCGCGCCT GGCCCAGCTT 
GCCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE2 ( XM_002694448.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002694494.2 Bos taurus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

Price & Availability↑

CloneID	OBa08968
Clone ID Related Accession (Same CDS sequence)	XM_591880.8 , XM_002694448.4
Accession Version	XM_002694448.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104452.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694448.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGC CGCGGCTGGC GGCGACTGAG TGGGCGGCGT TGGCCTGGGA GTTGCTGGGC 
GCCTCGGTGC TGCTGATCGC GGTGCGCTGG CTTGTGCGGC GGTTGGACAG GCGGCCGCGG 
GGCCTGGGTC AGAGCGGTCC ACCCGACCCG CCGTCCCGCG CGGTCGCGGG CCCAGCCCCG 
GATTCAGGTA AAATGACAAT GGATACCATC TTGGCGCGAT TGAAGCTCCT GAATCCAGAT 
GAGCTTAGAG AAGAGATTGT CAAAGCTGGA TTGAAATGTG GACCCATTAC ATCAACAACC 
AGGTTCATTT TTGAGAAAAA ACTGGCTCAG GCTTTATTAG AGCGTGGTAC ACTGCCTTCA 
CACCTGTCCG ACCCTGGTGC CGCAGATGCC CCATCCCTGG GCCAGGACAC ACAAAGGATT 
GTGAAGTCTG CAGTAGGGAG CCCAGCTGAG CAGGTCAGCT TTTCTGAAGA CAGAGACTTT 
GGTTATAGTG TGGGCTTGAA TCCTCCAGAG GAAGACGCCG TGACATCTAA GACCTGTTCA 
GCGCCCTTCA GTGCCTCAGG CGGCATCAAC AGCCAGAAAG CCGTGCTGAG GGCCTCTGCA 
GAGGTGCCTC TCTACTACGG GGTGTGCCCA GCGGCCTTCG AGGATACTCC AGCAAGAAAT 
GAAAGGATTC ACGTTTATGA AGATAAAAAG GAAGCCTTAC AAGCTGTCAA GATGAACAAA 
GGATCCCGAT TTAAAGCTTT TACAAACCGA GAAGATGCTG AGAAATTTGC CAGAGGAAGT 
TGTGATTATT TCCCTTCTCC AAGCAAAACC TCTTTACCAC TTTCTCCTGT GAAAACGGCA 
CCACTCTTTA GCAATGACGG GTTGTACTTG TCTGAGTCAG AAACAGCCAG CAAAGAGCGA 
GCAAACAGCT ATAAAAATCC ACGCACACAA GACCTCACTG CCAAACTTCG GAAAGCTGTG 
GAGAAGGGAG ACGAAGACAC CTTTTCCGAC CTCATCTGGA GTAATCCCCG GTATCTGATT 
GGTTCAGGGG ACAACCCAAC CATCGTACAG GAAGGGTGTC GGTACAATGT CATGCATGTG 
GCTGCCAAAG AGAACCAGGC TGCTGTGTGC CAGCTCACCC TAGAGACGCT GGAGAACCCC 
GAGTTCATGC GGCTCATGTA CCCAGATGAC GAGCCGCACA TGCTGGAGAA GCGCATTTGC 
TATGTGGTGG ACCTGTACCT CAACACACCG GACAGAGCGG GCTATGACAC ACCATTGCAT 
TTTGCTTGTA AGTTTGGAAA TGTAGATGTG GTCAATGTGC TTTCTTCACA CCCTTTGATT 
GTAAAAAACC CAAGGAATAA ATATGATAAA ACACCTGAAG ACGTTATTTG TGAAAGAAGC 
AAAAACAAAT CTGTAGAACT GAAGGAGCGG ATTCGAGACT ACTTAAAGGG TCACTACTAT 
GTGCCCCTCC TGAGGGCAGA GGACACGTCT TCTCCTGTGA TCGGGGAGCT GTGGTCTTCA 
GACCAGACAG GCGAGGTCTC TCACATTGGC CACACTGGAG GCTTCCCCAA AGACCCTGTT 
CTAACCCTTC GAGCCTTCGC AGGGCCCCTG AGTCCATCCA AGGCAGAAGA TTTTCGCCGA 
CTCTGGAAAA CCCCGCCTCG AGAGAAAGCG GGCTTCTTCC ACAGTGTCAG GAAATCTGAT 
CCAGAAAGAG GCATTGAGAG AGTGGGAAGG GAGCTGGCTC ATGAACTGGG GTATCCCTGG 
GTCGAATACT GGGACTTTCT GGGCTGTTTT GTTGATCTGT CTTCCCAGGC AGGCCTGCAA 
AAGCTGGAAG AATACCTTAC TCAGCAGGAA GTAGGCAAAA AGCACCTACA AGACATGGGA 
GAAAATGCAG CGTGTCGCCA GGAGGATGCC TCAGCCCTAG GCCGTCAGAG GAAGTGCAGC 
AATTCCATCT CTGTGAGGGC GTTTCTAGAT GAAGACGATG ACATGAGCTT GGAAGAGATA 
AAAAACCGGC AAAACACAGC TCGAAACAAC ACCCCGCCCA GGGCAGCTGC TTTTGGAGAC 
TTGGGGTGTG ACATCCTCCC CTTGGAGCAG ACAGAACTTA TCGAAGCCTC AGCCCCGACC 
AGTCCCCACA GCAGCAGAAA TGGGTTTTGC AGCCCTCTAA GGGATGGCAA GACCCTGGGT 
GGGAAGAGGC CCAAGGCCCA GAGTGGGGAG GAAGACCTCC GCTCACCCAT CTCCGGCTTG 
ACTGTTGAGT TTGATAAACT GAATTTGCAG AATCTAGAAA GTAGCTTTTC TAAGACACCA 
AATCAAAGAG TGAAAACTAG AGATAAGAAG GTCCTGACAT CAAGAGTGAA CGCGGGAGGA 
AGTGACGTGT TAGAACCTCT TGCTGCTGAT AGACTTGGAA ATAACCAGGG AAGGACAGAA 
AATGAGATGT CTGCTGGAAT TGCTAACATG TGCCTGAATC CAGACAGTTC TGTGCACGAG 
CCCCGCTATG GCAATGGCTC GGAGCCCTCG GAGATTCCCG CCTCAAAGGA GCCTGTCCAG 
AGGCTCTTCC TTTTTGGAGA AGAGCCGTCA AAACTGGATC GGGATGTTCT GGCAGCCCTG 
GAGTGCACGG ATGTGGACCC TCGCCAGTAC CCGGCCGTAC ACAGGTGGAG GAGCGCTGTC 
CTTTGCTACT CGCCTTCGGA CAGACAGAGC TGGTCCAGCC CCTCGCTGAA AGGGAAGTTG 
AAGTCTCAGC TCCTGGACGT CAGTAGCCCT CCTAGCGGCA GCCCTGGGAG AAGCAGCCCT 
GCAGGCAGCA GCCCTGGGAA GCCTGGCTCC ACACCTCACG CCTCCGGCCT GGGCAGCCCC 
GGGCGCTACA GTCCCGCCAA CGGGGGCCAC CTCCGCCGGA TGGCGCGCCT GGCCCAGCTT 
GCCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002694494.2
CDS	42..2933
Translation	MLWPRLAATEWAALAWELLGASVLLIAVRWLVRRLDRRPRGLGQSGPPDPPSRAVAGPAPDSGKMTMDTILARLKLLNPDELREEIVKAGLKCGPITSTTRFIFEKKLAQALLERGTLPSHLSDPGAADAPSLGQDTQRIVKSAVGSPAEQVSFSEDRDFGYSVGLNPPEEDAVTSKTCSAPFSASGGINSQKAVLRASAEVPLYYGVCPAAFEDTPARNERIHVYEDKKEALQAVKMNKGSRFKAFTNREDAEKFARGSCDYFPSPSKTSLPLSPVKTAPLFSNDGLYLSESETASKERANSYKNPRTQDLTAKLRKAVEKGDEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQAAVCQLTLETLENPEFMRLMYPDDEPHMLEKRICYVVDLYLNTPDRAGYDTPLHFACKFGNVDVVNVLSSHPLIVKNPRNKYDKTPEDVICERSKNKSVELKERIRDYLKGHYYVPLLRAEDTSSPVIGELWSSDQTGEVSHIGHTGGFPKDPVLTLRAFAGPLSPSKAEDFRRLWKTPPREKAGFFHSVRKSDPERGIERVGRELAHELGYPWVEYWDFLGCFVDLSSQAGLQKLEEYLTQQEVGKKHLQDMGENAACRQEDASALGRQRKCSNSISVRAFLDEDDDMSLEEIKNRQNTARNNTPPRAAAFGDLGCDILPLEQTELIEASAPTSPHSSRNGFCSPLRDGKTLGGKRPKAQSGEEDLRSPISGLTVEFDKLNLQNLESSFSKTPNQRVKTRDKKVLTSRVNAGGSDVLEPLAADRLGNNQGRTENEMSAGIANMCLNPDSSVHEPRYGNGSEPSEIPASKEPVQRLFLFGEEPSKLDRDVLAALECTDVDPRQYPAVHRWRSAVLCYSPSDRQSWSSPSLKGKLKSQLLDVSSPPSGSPGRSSPAGSSPGKPGSTPHASGLGSPGRYSPANGGHLRRMARLAQLATL

Target ORF information:

RefSeq Version	XM_002694448.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002694448.4

ORF Insert Sequence:

ATGCTGTGGC CGCGGCTGGC GGCGACTGAG TGGGCGGCGT TGGCCTGGGA GTTGCTGGGC 
GCCTCGGTGC TGCTGATCGC GGTGCGCTGG CTTGTGCGGC GGTTGGACAG GCGGCCGCGG 
GGCCTGGGTC AGAGCGGTCC ACCCGACCCG CCGTCCCGCG CGGTCGCGGG CCCAGCCCCG 
GATTCAGGTA AAATGACAAT GGATACCATC TTGGCGCGAT TGAAGCTCCT GAATCCAGAT 
GAGCTTAGAG AAGAGATTGT CAAAGCTGGA TTGAAATGTG GACCCATTAC ATCAACAACC 
AGGTTCATTT TTGAGAAAAA ACTGGCTCAG GCTTTATTAG AGCGTGGTAC ACTGCCTTCA 
CACCTGTCCG ACCCTGGTGC CGCAGATGCC CCATCCCTGG GCCAGGACAC ACAAAGGATT 
GTGAAGTCTG CAGTAGGGAG CCCAGCTGAG CAGGTCAGCT TTTCTGAAGA CAGAGACTTT 
GGTTATAGTG TGGGCTTGAA TCCTCCAGAG GAAGACGCCG TGACATCTAA GACCTGTTCA 
GCGCCCTTCA GTGCCTCAGG CGGCATCAAC AGCCAGAAAG CCGTGCTGAG GGCCTCTGCA 
GAGGTGCCTC TCTACTACGG GGTGTGCCCA GCGGCCTTCG AGGATACTCC AGCAAGAAAT 
GAAAGGATTC ACGTTTATGA AGATAAAAAG GAAGCCTTAC AAGCTGTCAA GATGAACAAA 
GGATCCCGAT TTAAAGCTTT TACAAACCGA GAAGATGCTG AGAAATTTGC CAGAGGAAGT 
TGTGATTATT TCCCTTCTCC AAGCAAAACC TCTTTACCAC TTTCTCCTGT GAAAACGGCA 
CCACTCTTTA GCAATGACGG GTTGTACTTG TCTGAGTCAG AAACAGCCAG CAAAGAGCGA 
GCAAACAGCT ATAAAAATCC ACGCACACAA GACCTCACTG CCAAACTTCG GAAAGCTGTG 
GAGAAGGGAG ACGAAGACAC CTTTTCCGAC CTCATCTGGA GTAATCCCCG GTATCTGATT 
GGTTCAGGGG ACAACCCAAC CATCGTACAG GAAGGGTGTC GGTACAATGT CATGCATGTG 
GCTGCCAAAG AGAACCAGGC TGCTGTGTGC CAGCTCACCC TAGAGACGCT GGAGAACCCC 
GAGTTCATGC GGCTCATGTA CCCAGATGAC GAGCCGCACA TGCTGGAGAA GCGCATTTGC 
TATGTGGTGG ACCTGTACCT CAACACACCG GACAGAGCGG GCTATGACAC ACCATTGCAT 
TTTGCTTGTA AGTTTGGAAA TGTAGATGTG GTCAATGTGC TTTCTTCACA CCCTTTGATT 
GTAAAAAACC CAAGGAATAA ATATGATAAA ACACCTGAAG ACGTTATTTG TGAAAGAAGC 
AAAAACAAAT CTGTAGAACT GAAGGAGCGG ATTCGAGACT ACTTAAAGGG TCACTACTAT 
GTGCCCCTCC TGAGGGCAGA GGACACGTCT TCTCCTGTGA TCGGGGAGCT GTGGTCTTCA 
GACCAGACAG GCGAGGTCTC TCACATTGGC CACACTGGAG GCTTCCCCAA AGACCCTGTT 
CTAACCCTTC GAGCCTTCGC AGGGCCCCTG AGTCCATCCA AGGCAGAAGA TTTTCGCCGA 
CTCTGGAAAA CCCCGCCTCG AGAGAAAGCG GGCTTCTTCC ACAGTGTCAG GAAATCTGAT 
CCAGAAAGAG GCATTGAGAG AGTGGGAAGG GAGCTGGCTC ATGAACTGGG GTATCCCTGG 
GTCGAATACT GGGACTTTCT GGGCTGTTTT GTTGATCTGT CTTCCCAGGC AGGCCTGCAA 
AAGCTGGAAG AATACCTTAC TCAGCAGGAA GTAGGCAAAA AGCACCTACA AGACATGGGA 
GAAAATGCAG CGTGTCGCCA GGAGGATGCC TCAGCCCTAG GCCGTCAGAG GAAGTGCAGC 
AATTCCATCT CTGTGAGGGC GTTTCTAGAT GAAGACGATG ACATGAGCTT GGAAGAGATA 
AAAAACCGGC AAAACACAGC TCGAAACAAC ACCCCGCCCA GGGCAGCTGC TTTTGGAGAC 
TTGGGGTGTG ACATCCTCCC CTTGGAGCAG ACAGAACTTA TCGAAGCCTC AGCCCCGACC 
AGTCCCCACA GCAGCAGAAA TGGGTTTTGC AGCCCTCTAA GGGATGGCAA GACCCTGGGT 
GGGAAGAGGC CCAAGGCCCA GAGTGGGGAG GAAGACCTCC GCTCACCCAT CTCCGGCTTG 
ACTGTTGAGT TTGATAAACT GAATTTGCAG AATCTAGAAA GTAGCTTTTC TAAGACACCA 
AATCAAAGAG TGAAAACTAG AGATAAGAAG GTCCTGACAT CAAGAGTGAA CGCGGGAGGA 
AGTGACGTGT TAGAACCTCT TGCTGCTGAT AGACTTGGAA ATAACCAGGG AAGGACAGAA 
AATGAGATGT CTGCTGGAAT TGCTAACATG TGCCTGAATC CAGACAGTTC TGTGCACGAG 
CCCCGCTATG GCAATGGCTC GGAGCCCTCG GAGATTCCCG CCTCAAAGGA GCCTGTCCAG 
AGGCTCTTCC TTTTTGGAGA AGAGCCGTCA AAACTGGATC GGGATGTTCT GGCAGCCCTG 
GAGTGCACGG ATGTGGACCC TCGCCAGTAC CCGGCCGTAC ACAGGTGGAG GAGCGCTGTC 
CTTTGCTACT CGCCTTCGGA CAGACAGAGC TGGTCCAGCC CCTCGCTGAA AGGGAAGTTG 
AAGTCTCAGC TCCTGGACGT CAGTAGCCCT CCTAGCGGCA GCCCTGGGAG AAGCAGCCCT 
GCAGGCAGCA GCCCTGGGAA GCCTGGCTCC ACACCTCACG CCTCCGGCCT GGGCAGCCCC 
GGGCGCTACA GTCCCGCCAA CGGGGGCCAC CTCCGCCGGA TGGCGCGCCT GGCCCAGCTT 
GCCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE2 ( XM_002694448.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002694494.3 Bos taurus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

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CloneID	OBa224548
Clone ID Related Accession (Same CDS sequence)	XM_002694448.5
Accession Version	XM_002694448.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694448.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGC CGCGGCTGGC GGCGACTGAG TGGGCGGCGT TGGCCTGGGA GTTGCTGGGC 
GCCTCGGTGC TGCTGATCGC GGTGCGCTGG CTTGTGCGGC GGTTGGACAG GCGGCCGCGG 
GGCCTGGGTC AGAGCGGTCC ACCCGACCCG CCGTCCCGCG CGGTCGCGGG CCCAGCCCCG 
GATTCAGGTA AAATGACAAT GGATACCATC TTGGCGCGAT TGAAGCTCCT GAATCCAGAT 
GAGCTTAGAG AAGAGATTGT CAAAGCTGGA TTGAAATGTG GACCCATTAC ATCAACAACC 
AGGTTCATTT TTGAGAAAAA ACTGGCTCAG GCTTTATTAG AGCATGGTAC ACTGCCTTCA 
CACCTGTCCG ACCCTGGTGC CGCAGATGCC CCATCCCTGG GCCAGGACAC ACAAAGGATT 
GTGAAGTCTG CAGTAGGGAG CCCAGCTGAG CAGGTCAGCT TTTCTGAAGA CAGAGACTTT 
GGTTATAGTG TGGGCTTGAA TCCTCCAGAG GAAGACGCCG TGACATCTAA GACCTGTTCA 
GCGCCCTTCA GTGCCTCAGG CGGCATCAAC AGCCAGAAAG CCGTGCTGAG GGCCTCTGCA 
GAGGTGCCTC TCTACTACGG GGTGTGCCCA GCGGCCTTCG AGGATACTCC AGCAAGAAAT 
GAAAGGATTC ACGTTTATGA AGATAAAAAG GAAGCCTTAC AAGCTGTCAA GATGAACAAA 
GGATCCCGAT TTAAAGCTTT TACAAACCGA GAAGATGCTG AGAAATTTGC CAGAGGAAGT 
TGTGATTATT TCCCTTCTCC AAGCAAAACC TCTTTACCAC TTTCTCCTGT GAAAACGGCA 
CCACTCTTTA GCAATGACGG GTTGTACTTG TCTGAGTCAG AAACAGCCAG CAAAGAGCGA 
GCAAACAGCT ATAAAAATCC ACGCACACAA GACCTCACTG CCAAACTTCG GAAAGCTGTG 
GAGAAGGGAG ACGAAGACAC CTTTTCCGAC CTCATCTGGA GTAATCCCCG GTATCTGATT 
GGTTCAGGGG ACAACCCAAC CATCGTACAG GAAGGGTGTC GGTACAATGT CATGCATGTG 
GCTGCCAAAG AGAACCAGGC TGCTGTGTGC CAGCTCACCC TAGAGACGCT GGAGAACCCC 
GAGTTCATGC GGCTCATGTA CCCAGATGAC GAGCCGCACA TGCTGGAGAA GCGCATTTGC 
TATGTGGTGG ACCTGTACCT CAACACACCG GACAGAGCGG GCTATGACAC ACCATTGCAT 
TTTGCTTGTA AGTTTGGAAA TGTAGATGTG GTCAATGTGC TTTCTTCACA CCCTTTGATT 
GTAAAAAACC CAAGGAATAA ATATGATAAA ACACCTGAAG ACGTTATTTG TGAAAGAAGC 
AAAAACAAAT CTGTAGAACT GAAGGAGCGG ATTCGAGACT ACTTAAAGGG TCACTACTAT 
GTGCCCCTCC TGAGGGCAGA GGACACGTCT TCTCCTGTGA TCGGGGAGCT GTGGTCTTCA 
GACCAGACAG GCGAGGTCTC TCACATTGGC CACACTGGAG GCTTCCCCAA AGACCCTGTT 
CTAACCCTTC GAGCCTTCGC AGGGCCCCTG AGTCCATCCA AGGCAGAAGA TTTTCGCCGA 
CTCTGGAAAA CCCCGCCTCG AGAGAAAGCG GGCTTCTTCC ACAGTGTCAG GAAATCTGAT 
CCAGAAAGAG GCATTGAGAG AGTGGGAAGG GAGCTGGCTC ATGAACTGGG GTATCCCTGG 
GTCGAATACT GGGACTTTCT GGGCTGTTTT GTTGATCTGT CTTCCCAGGC AGGCCTGCAA 
AAGCTGGAAG AATACCTTAC TCAGCAGGAA GTAGGCAAAA AGCACCTACA AGACATGGGA 
GAAAATGCAG CGTGTCGCCA GGAGGATGCC TCAGCCCTAG GCCGTCAGAG GAAGTGCAGC 
AATTCCATCT CTGTGAGGGC GTTTCTAGAT GAAGACGATG ACATGAGCTT GGAAGAGATA 
AAAAACCGGC AAAACACAGC TCGAAACAAC ACCCCGCCCA GGGCAGCTGC TTTTGGAGAC 
TTGGGGTGTG ACATCCTCCC CTTGGAGCAG ACAGAACTTA TCGAAGCCTC AGCCCCGACC 
AGTCCCCACA GCAGCAGAAA TGGGTTTTGC AGCCCTCTAA GGGATGGCAA GACCCTGGGT 
GGGAAGAGGC CCAAGGCCCA GAGTGGGGAG GAAGACCTCC GCTCACCCAT CTCCGGCTTG 
ACTGTTGAGT TTGATAAACT GAATTTGCAG AATCTAGAAA GTAGCTTTTC TAAGACACCA 
AATCAAAGAG TGAAAACTAG AGATAAGAAG GTCCTGACAT CAAGAGTGAA CGCGGGAGGA 
AGTGACGTGT TAGAACCTCT TGCTGCTGAT AGACTTGGAA ATAACCAGGG AAGGACAGAA 
AATGAGATGT CTGCTGGAAT TGCTAACATG TGCCTGAATC CAGACAGTTC TGTGCACGAG 
CCCCGCTATG GCAATGGCTC GGAGCCCTCG GAGATTCCCG CCTCAAAGGA GCCTGTCCAG 
AGGCTCTTCC TTTTTGGAGA AGAGCCGTCA AAACTGGATC GGGATGTTCT GGCAGCCCTG 
GAGTGCACGG ATGTGGACCC TCGCCAGTAC CCGGCCGTAC ACAGGTGGAG GAGCGCTGTC 
CTTTGCTACT CGCCTTCGGA CAGACAGAGC TGGTCCAGCC CCTCGCTGAA AGGGAAGTTG 
AAGTCTCAGC TCCTGGACGT CAGTAGCCCT CCTAGCGGCA GCCCTGGGAG AAGCAGCCCT 
GCAGGCAGCA GCCCTGGGAA GCCTGGCTCC ACACCTCACG CCTCCGGCCT GGGCAGCCCC 
GGGCGCTACA GTCCCGCCAA CGGGGGCCAC CTCCGCCGGA TGGCGCGCCT GGCCCAGCTT 
GCCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002694494.3
CDS	68..2959
Translation	MLWPRLAATEWAALAWELLGASVLLIAVRWLVRRLDRRPRGLGQSGPPDPPSRAVAGPAPDSGKMTMDTILARLKLLNPDELREEIVKAGLKCGPITSTTRFIFEKKLAQALLEHGTLPSHLSDPGAADAPSLGQDTQRIVKSAVGSPAEQVSFSEDRDFGYSVGLNPPEEDAVTSKTCSAPFSASGGINSQKAVLRASAEVPLYYGVCPAAFEDTPARNERIHVYEDKKEALQAVKMNKGSRFKAFTNREDAEKFARGSCDYFPSPSKTSLPLSPVKTAPLFSNDGLYLSESETASKERANSYKNPRTQDLTAKLRKAVEKGDEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQAAVCQLTLETLENPEFMRLMYPDDEPHMLEKRICYVVDLYLNTPDRAGYDTPLHFACKFGNVDVVNVLSSHPLIVKNPRNKYDKTPEDVICERSKNKSVELKERIRDYLKGHYYVPLLRAEDTSSPVIGELWSSDQTGEVSHIGHTGGFPKDPVLTLRAFAGPLSPSKAEDFRRLWKTPPREKAGFFHSVRKSDPERGIERVGRELAHELGYPWVEYWDFLGCFVDLSSQAGLQKLEEYLTQQEVGKKHLQDMGENAACRQEDASALGRQRKCSNSISVRAFLDEDDDMSLEEIKNRQNTARNNTPPRAAAFGDLGCDILPLEQTELIEASAPTSPHSSRNGFCSPLRDGKTLGGKRPKAQSGEEDLRSPISGLTVEFDKLNLQNLESSFSKTPNQRVKTRDKKVLTSRVNAGGSDVLEPLAADRLGNNQGRTENEMSAGIANMCLNPDSSVHEPRYGNGSEPSEIPASKEPVQRLFLFGEEPSKLDRDVLAALECTDVDPRQYPAVHRWRSAVLCYSPSDRQSWSSPSLKGKLKSQLLDVSSPPSGSPGRSSPAGSSPGKPGSTPHASGLGSPGRYSPANGGHLRRMARLAQLATL

Target ORF information:

RefSeq Version	XM_002694448.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002694448.5

ORF Insert Sequence:

ATGCTGTGGC CGCGGCTGGC GGCGACTGAG TGGGCGGCGT TGGCCTGGGA GTTGCTGGGC 
GCCTCGGTGC TGCTGATCGC GGTGCGCTGG CTTGTGCGGC GGTTGGACAG GCGGCCGCGG 
GGCCTGGGTC AGAGCGGTCC ACCCGACCCG CCGTCCCGCG CGGTCGCGGG CCCAGCCCCG 
GATTCAGGTA AAATGACAAT GGATACCATC TTGGCGCGAT TGAAGCTCCT GAATCCAGAT 
GAGCTTAGAG AAGAGATTGT CAAAGCTGGA TTGAAATGTG GACCCATTAC ATCAACAACC 
AGGTTCATTT TTGAGAAAAA ACTGGCTCAG GCTTTATTAG AGCATGGTAC ACTGCCTTCA 
CACCTGTCCG ACCCTGGTGC CGCAGATGCC CCATCCCTGG GCCAGGACAC ACAAAGGATT 
GTGAAGTCTG CAGTAGGGAG CCCAGCTGAG CAGGTCAGCT TTTCTGAAGA CAGAGACTTT 
GGTTATAGTG TGGGCTTGAA TCCTCCAGAG GAAGACGCCG TGACATCTAA GACCTGTTCA 
GCGCCCTTCA GTGCCTCAGG CGGCATCAAC AGCCAGAAAG CCGTGCTGAG GGCCTCTGCA 
GAGGTGCCTC TCTACTACGG GGTGTGCCCA GCGGCCTTCG AGGATACTCC AGCAAGAAAT 
GAAAGGATTC ACGTTTATGA AGATAAAAAG GAAGCCTTAC AAGCTGTCAA GATGAACAAA 
GGATCCCGAT TTAAAGCTTT TACAAACCGA GAAGATGCTG AGAAATTTGC CAGAGGAAGT 
TGTGATTATT TCCCTTCTCC AAGCAAAACC TCTTTACCAC TTTCTCCTGT GAAAACGGCA 
CCACTCTTTA GCAATGACGG GTTGTACTTG TCTGAGTCAG AAACAGCCAG CAAAGAGCGA 
GCAAACAGCT ATAAAAATCC ACGCACACAA GACCTCACTG CCAAACTTCG GAAAGCTGTG 
GAGAAGGGAG ACGAAGACAC CTTTTCCGAC CTCATCTGGA GTAATCCCCG GTATCTGATT 
GGTTCAGGGG ACAACCCAAC CATCGTACAG GAAGGGTGTC GGTACAATGT CATGCATGTG 
GCTGCCAAAG AGAACCAGGC TGCTGTGTGC CAGCTCACCC TAGAGACGCT GGAGAACCCC 
GAGTTCATGC GGCTCATGTA CCCAGATGAC GAGCCGCACA TGCTGGAGAA GCGCATTTGC 
TATGTGGTGG ACCTGTACCT CAACACACCG GACAGAGCGG GCTATGACAC ACCATTGCAT 
TTTGCTTGTA AGTTTGGAAA TGTAGATGTG GTCAATGTGC TTTCTTCACA CCCTTTGATT 
GTAAAAAACC CAAGGAATAA ATATGATAAA ACACCTGAAG ACGTTATTTG TGAAAGAAGC 
AAAAACAAAT CTGTAGAACT GAAGGAGCGG ATTCGAGACT ACTTAAAGGG TCACTACTAT 
GTGCCCCTCC TGAGGGCAGA GGACACGTCT TCTCCTGTGA TCGGGGAGCT GTGGTCTTCA 
GACCAGACAG GCGAGGTCTC TCACATTGGC CACACTGGAG GCTTCCCCAA AGACCCTGTT 
CTAACCCTTC GAGCCTTCGC AGGGCCCCTG AGTCCATCCA AGGCAGAAGA TTTTCGCCGA 
CTCTGGAAAA CCCCGCCTCG AGAGAAAGCG GGCTTCTTCC ACAGTGTCAG GAAATCTGAT 
CCAGAAAGAG GCATTGAGAG AGTGGGAAGG GAGCTGGCTC ATGAACTGGG GTATCCCTGG 
GTCGAATACT GGGACTTTCT GGGCTGTTTT GTTGATCTGT CTTCCCAGGC AGGCCTGCAA 
AAGCTGGAAG AATACCTTAC TCAGCAGGAA GTAGGCAAAA AGCACCTACA AGACATGGGA 
GAAAATGCAG CGTGTCGCCA GGAGGATGCC TCAGCCCTAG GCCGTCAGAG GAAGTGCAGC 
AATTCCATCT CTGTGAGGGC GTTTCTAGAT GAAGACGATG ACATGAGCTT GGAAGAGATA 
AAAAACCGGC AAAACACAGC TCGAAACAAC ACCCCGCCCA GGGCAGCTGC TTTTGGAGAC 
TTGGGGTGTG ACATCCTCCC CTTGGAGCAG ACAGAACTTA TCGAAGCCTC AGCCCCGACC 
AGTCCCCACA GCAGCAGAAA TGGGTTTTGC AGCCCTCTAA GGGATGGCAA GACCCTGGGT 
GGGAAGAGGC CCAAGGCCCA GAGTGGGGAG GAAGACCTCC GCTCACCCAT CTCCGGCTTG 
ACTGTTGAGT TTGATAAACT GAATTTGCAG AATCTAGAAA GTAGCTTTTC TAAGACACCA 
AATCAAAGAG TGAAAACTAG AGATAAGAAG GTCCTGACAT CAAGAGTGAA CGCGGGAGGA 
AGTGACGTGT TAGAACCTCT TGCTGCTGAT AGACTTGGAA ATAACCAGGG AAGGACAGAA 
AATGAGATGT CTGCTGGAAT TGCTAACATG TGCCTGAATC CAGACAGTTC TGTGCACGAG 
CCCCGCTATG GCAATGGCTC GGAGCCCTCG GAGATTCCCG CCTCAAAGGA GCCTGTCCAG 
AGGCTCTTCC TTTTTGGAGA AGAGCCGTCA AAACTGGATC GGGATGTTCT GGCAGCCCTG 
GAGTGCACGG ATGTGGACCC TCGCCAGTAC CCGGCCGTAC ACAGGTGGAG GAGCGCTGTC 
CTTTGCTACT CGCCTTCGGA CAGACAGAGC TGGTCCAGCC CCTCGCTGAA AGGGAAGTTG 
AAGTCTCAGC TCCTGGACGT CAGTAGCCCT CCTAGCGGCA GCCCTGGGAG AAGCAGCCCT 
GCAGGCAGCA GCCCTGGGAA GCCTGGCTCC ACACCTCACG CCTCCGGCCT GGGCAGCCCC 
GGGCGCTACA GTCCCGCCAA CGGGGGCCAC CTCCGCCGGA TGGCGCGCCT GGCCCAGCTT 
GCCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ANKLE2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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