ZAR1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZAR1
Entrez Gene ID	540150
Full Name	zygote arrest 1
General protein information	Preferred Names zygote arrest 1 Names zygote arrest protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024993375.1	XP_024849143.1	zygote arrest protein 1 isoform X1
XM_024993376.1	XP_024849144.1	zygote arrest protein 1 isoform X2
NM_001076203.1	NP_001069671.1	zygote arrest protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZAR1	NP_783318.1
Macaca mulatta (Rhesus monkey)	ZAR1	XP_001103446.1
Rattus norvegicus (Norway rat)	Zar1	NP_852050.1
Gallus gallus (chicken)	ZAR1	XP_003641256.2
Mus musculus (house mouse)	Zar1	NP_777366.1
Pan troglodytes (chimpanzee)	ZAR1	XP_001151745.1
Bos taurus (cattle)	ZAR1	NP_001069671.1
Canis lupus familiaris (dog)	ZAR1	XP_003431884.1
Xenopus tropicalis (tropical clawed frog)	zar1	NP_001016947.2
Danio rerio (zebrafish)	zar1	NP_919362.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Zygote arrest 1 gene in pig, cattle and human: evidence of different transcript variants in male and female germ cells.
Uzbekova S, Roy-Sabau M, Dalbiès-Tran R, Perreau C, Papillier P, Mompart F, Thelie A, Pennetier S, Cognie J, Cadoret V, Royere D, Monget P, Mermillod P
Reproductive biology and endocrinology : RB&E412(2006 Mar)

Expression pattern of the maternal factor zygote arrest 1 (Zar1) in bovine tissues, oocytes, and embryos.
Brevini TA, Cillo F, Colleoni S, Lazzari G, Galli C, Gandolfi F
Molecular reproduction and development69(4)375-80(2004 Dec)

Spatio-temporal expression of the germ cell marker genes MATER, ZAR1, GDF9, BMP15,andVASA in adult bovine tissues, oocytes, and preimplantation embryos.
Pennetier S, Uzbekova S, Perreau C, Papillier P, Mermillod P, Dalbiès-Tran R
Biology of reproduction71(4)1359-66(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ZAR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZAR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36577	NM_001076203.1 Latest version!	Bos taurus zygote arrest 1 (ZAR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa235416	XM_024993375.1 Latest version!	Bos taurus zygote arrest 1 (ZAR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa235417	XM_024993376.1 Latest version!	Bos taurus zygote arrest 1 (ZAR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZAR1 ( NM_001076203.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069671.1 Bos taurus zygote arrest 1 (ZAR1), mRNA.

Price & Availability↑

CloneID	OBa36577
Clone ID Related Accession (Same CDS sequence)	NM_001076203.1
Accession Version	NM_001076203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	zygote arrest protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ231456.1. On Oct 4, 2006 this sequence version replaced XM_591835.2. ##Evidence-Data-START## Transcript exon combination :: DQ231456.1, DQ231448.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145443, SAMN03145450 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCCC TGGGGGACGT CGTCCTGGAT GGTTACCTGT ACCCGGCGTG CGCTCTCTAC 
TCGTACCGGT GCCTCTACCC GGCCGCCGCC GCCGCCAAGG GAAAAAGCGG GGCGGACGAG 
GGTGGCTGGC GACCCCGGGG CGGGGGCTAC CCTCCCGTTT CCTCCTCCTC TGACGGCGCG 
GCCTCGTCGT CGTTCCCGGG CCACGGGCAG CTGGCGGCCG CCGAGTACGT CCACAGCTAC 
CAGCGCGCGC AGCTCATGGC CCTGCTGTCG CAGGTGGGCC CCCGCCCGGC CAGCACTCGG 
GACGCGGCGG TGCAGGTGAA CCCGTTCCGC GACGTATCGG TGCAGTGCTC GCTGGGGCGG 
CGGACGCTGG GGCACAGGGC CCGCGAGTCC GGCCCGAGTC CGGATCCCGA GGGCGCGGCG 
GACGCGGGCG GCAGCTGCCC GGCCTCCCCG CAGCGCGCCC GCCGGGGCCC GGAGCAGGAC 
AGCCCGCCGA GCCGCGCCCC GCGGCGCGTG CGTTTCCTGC GCACCCTGGC TGTGTACTCG 
CCCGTGACCT CCCGCTGCCT AGCCACCCTC CTGGAGGGGG CCGAGGCCGT GGCGGGCCAG 
CAGAGGCCCG GGGAGCCGGA GACTGAGCGA GGGCCGCCAC CTGCGAGGCC CCGAGGCCCC 
GAGGAGGGAG ACGGGTCGGC GAGGAAGGTT TCCCTGCAGC TGCAGCCTGA GGAGGACGAG 
GCCCAGGCCG CAGTACCGGC AAGCCGCGAG CAGCCCCCGC CCGTTGCCAG GGTCCCGGAC 
ACCGCTGGCG AGAGATCGTC GCCCCGGAGC CCCCAGCCGA GCAAGGAGCG CCTGCGCTTC 
CAGTTCTTAG AGCAGAAGTA CGGCTACTAT CACTGCAAGG ACTGCAATAT CCGCTGGGAA 
AGTGCCTATG TGTGGTGTGT ACAGGGCACT AACAAGGTTT ACTACAAGCA GTTTTGCCGA 
ACATGCCAGA AGTCTTATAA CCCTTACCGA GTGGAGGATA TCACCTGCCA AAATTGTAAA 
CAGACTAGAT GCTCCTGCCC AGTGAAACTT CGCCATGTGG ACCCCAAAAG GCCCCACCGT 
CAAGATTTGT GTGGGAGATG CAAAGGCAAA CGCCTATCCT GTGATAGCAC TTTCAGTTTC 
AAATATATCA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069671.1
CDS	1..1155
Translation	MAALGDVVLDGYLYPACALYSYRCLYPAAAAAKGKSGADEGGWRPRGGGYPPVSSSSDGAASSSFPGHGQLAAAEYVHSYQRAQLMALLSQVGPRPASTRDAAVQVNPFRDVSVQCSLGRRTLGHRARESGPSPDPEGAADAGGSCPASPQRARRGPEQDSPPSRAPRRVRFLRTLAVYSPVTSRCLATLLEGAEAVAGQQRPGEPETERGPPPARPRGPEEGDGSARKVSLQLQPEEDEAQAAVPASREQPPPVARVPDTAGERSSPRSPQPSKERLRFQFLEQKYGYYHCKDCNIRWESAYVWCVQGTNKVYYKQFCRTCQKSYNPYRVEDITCQNCKQTRCSCPVKLRHVDPKRPHRQDLCGRCKGKRLSCDSTFSFKYII

Target ORF information:

RefSeq Version	NM_001076203.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zygote arrest 1 (ZAR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076203.1

ORF Insert Sequence:

ATGGCTGCCC TGGGGGACGT CGTCCTGGAT GGTTACCTGT ACCCGGCGTG CGCTCTCTAC 
TCGTACCGGT GCCTCTACCC GGCCGCCGCC GCCGCCAAGG GAAAAAGCGG GGCGGACGAG 
GGTGGCTGGC GACCCCGGGG CGGGGGCTAC CCTCCCGTTT CCTCCTCCTC TGACGGCGCG 
GCCTCGTCGT CGTTCCCGGG CCACGGGCAG CTGGCGGCCG CCGAGTACGT CCACAGCTAC 
CAGCGCGCGC AGCTCATGGC CCTGCTGTCG CAGGTGGGCC CCCGCCCGGC CAGCACTCGG 
GACGCGGCGG TGCAGGTGAA CCCGTTCCGC GACGTATCGG TGCAGTGCTC GCTGGGGCGG 
CGGACGCTGG GGCACAGGGC CCGCGAGTCC GGCCCGAGTC CGGATCCCGA GGGCGCGGCG 
GACGCGGGCG GCAGCTGCCC GGCCTCCCCG CAGCGCGCCC GCCGGGGCCC GGAGCAGGAC 
AGCCCGCCGA GCCGCGCCCC GCGGCGCGTG CGTTTCCTGC GCACCCTGGC TGTGTACTCG 
CCCGTGACCT CCCGCTGCCT AGCCACCCTC CTGGAGGGGG CCGAGGCCGT GGCGGGCCAG 
CAGAGGCCCG GGGAGCCGGA GACTGAGCGA GGGCCGCCAC CTGCGAGGCC CCGAGGCCCC 
GAGGAGGGAG ACGGGTCGGC GAGGAAGGTT TCCCTGCAGC TGCAGCCTGA GGAGGACGAG 
GCCCAGGCCG CAGTACCGGC AAGCCGCGAG CAGCCCCCGC CCGTTGCCAG GGTCCCGGAC 
ACCGCTGGCG AGAGATCGTC GCCCCGGAGC CCCCAGCCGA GCAAGGAGCG CCTGCGCTTC 
CAGTTCTTAG AGCAGAAGTA CGGCTACTAT CACTGCAAGG ACTGCAATAT CCGCTGGGAA 
AGTGCCTATG TGTGGTGTGT ACAGGGCACT AACAAGGTTT ACTACAAGCA GTTTTGCCGA 
ACATGCCAGA AGTCTTATAA CCCTTACCGA GTGGAGGATA TCACCTGCCA AAATTGTAAA 
CAGACTAGAT GCTCCTGCCC AGTGAAACTT CGCCATGTGG ACCCCAAAAG GCCCCACCGT 
CAAGATTTGT GTGGGAGATG CAAAGGCAAA CGCCTATCCT GTGATAGCAC TTTCAGTTTC 
AAATATATCA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZAR1 ( XM_024993375.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849143.1 Bos taurus zygote arrest 1 (ZAR1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235416
Clone ID Related Accession (Same CDS sequence)	XM_024993375.1
Accession Version	XM_024993375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	zygote arrest protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC TGGGGGACGT CGTCCTGGAT GGTTACCTGT ACCCGGCGTG CGCTCTCTAC 
TCGTACCGGT GCCTCTACCC GGCCGCCGCC GCCGCCAAGG GAAAAAGCGG GGCGGACGAG 
GGTGGCTGGC GACCCCGGGG CGGGGGCTAC CCTCCCGTTT CCTCCTCCTC TGACGGCGCG 
GCCTCGTCGT CGTTCCCGGG CCACGGGCAG CTGGCGGCCG CCGAGTACGT CCACAGCTAC 
CAGCGCGCGC AGCTCATGGC CCTGCTGTCG CAGGTGGGCC CCCGCCCGGC CAGCACTCGG 
GACGCGGCGG TGCAGGTGAA CCCGTTCCGC GACGTATCGG TGCAGTGCTC GCTGGGGCGG 
CGGACGCTGG GGCACAGGGC CCGCGAGTCC GGCCCGAGTC CGGATCCCGA GGGCGCGGCG 
GACGCGGGCG GCAGCTGCCC GGCCTCCCCG CAGCGCGCCC GCCGGGGCCC GGAGCAGGAC 
AGCCCGCCGA GCCGCGCCCC GCGGCGCGTG CGTTTCCTGC GCACCCTGGC TGTGTACTCG 
CCCGTGACCT CCCGCTGCCT AGCCACCCTC CTGGAGGGGG CCGAGGCCGT GGCGGGCCAG 
CAGAGGCCCG GGGAGCCGGA GACTGAGCGA GGGCCGCCAC CTGCGAGGCC CCGAGGCCCC 
GAGGAGGGAG ACGGGTCGGC GAGGAAGGTT TCCCTGCAGC TGCAGCCTGA GGAGGACGAG 
GCCCAGGCCG CAGTACCGGC AAGCCGCGAG CAGCCCCCGC CCGTTGCCAG GGTCCCGGAC 
ACCGCTGGCG AGAGATCGTC GCCCCGGAGC CCCCAGCCGA GCAAGGAGCG CCTGCGCTTC 
CAGTTCTTAG AGCAGAAGTA CGGCTACTAT CACTGCAAGG ACTGCAATAT CCGCTGGGAA 
AGTGCCTATG TGTGGTGTGT ACAGGGCACT AACAAGGTTT ACTACAAGCA GTTTTGCCGA 
ACATGCCAGA AGTCTTATAA CCCTTACCGA GTGGAGGATA TCACCTGCCA AATTATGCTG 
GTGGATAAGC AGTTCTCAGA AACCAAAAGG AGGGATGGAT GCTCGCTCTG GCTCTGGATT 
GGTTGTAGAC ACAGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849143.1
CDS	39..1139
Translation	MAALGDVVLDGYLYPACALYSYRCLYPAAAAAKGKSGADEGGWRPRGGGYPPVSSSSDGAASSSFPGHGQLAAAEYVHSYQRAQLMALLSQVGPRPASTRDAAVQVNPFRDVSVQCSLGRRTLGHRARESGPSPDPEGAADAGGSCPASPQRARRGPEQDSPPSRAPRRVRFLRTLAVYSPVTSRCLATLLEGAEAVAGQQRPGEPETERGPPPARPRGPEEGDGSARKVSLQLQPEEDEAQAAVPASREQPPPVARVPDTAGERSSPRSPQPSKERLRFQFLEQKYGYYHCKDCNIRWESAYVWCVQGTNKVYYKQFCRTCQKSYNPYRVEDITCQIMLVDKQFSETKRRDGCSLWLWIGCRHRL

Target ORF information:

RefSeq Version	XM_024993375.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zygote arrest 1 (ZAR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993375.1

ORF Insert Sequence:

ATGGCTGCCC TGGGGGACGT CGTCCTGGAT GGTTACCTGT ACCCGGCGTG CGCTCTCTAC 
TCGTACCGGT GCCTCTACCC GGCCGCCGCC GCCGCCAAGG GAAAAAGCGG GGCGGACGAG 
GGTGGCTGGC GACCCCGGGG CGGGGGCTAC CCTCCCGTTT CCTCCTCCTC TGACGGCGCG 
GCCTCGTCGT CGTTCCCGGG CCACGGGCAG CTGGCGGCCG CCGAGTACGT CCACAGCTAC 
CAGCGCGCGC AGCTCATGGC CCTGCTGTCG CAGGTGGGCC CCCGCCCGGC CAGCACTCGG 
GACGCGGCGG TGCAGGTGAA CCCGTTCCGC GACGTATCGG TGCAGTGCTC GCTGGGGCGG 
CGGACGCTGG GGCACAGGGC CCGCGAGTCC GGCCCGAGTC CGGATCCCGA GGGCGCGGCG 
GACGCGGGCG GCAGCTGCCC GGCCTCCCCG CAGCGCGCCC GCCGGGGCCC GGAGCAGGAC 
AGCCCGCCGA GCCGCGCCCC GCGGCGCGTG CGTTTCCTGC GCACCCTGGC TGTGTACTCG 
CCCGTGACCT CCCGCTGCCT AGCCACCCTC CTGGAGGGGG CCGAGGCCGT GGCGGGCCAG 
CAGAGGCCCG GGGAGCCGGA GACTGAGCGA GGGCCGCCAC CTGCGAGGCC CCGAGGCCCC 
GAGGAGGGAG ACGGGTCGGC GAGGAAGGTT TCCCTGCAGC TGCAGCCTGA GGAGGACGAG 
GCCCAGGCCG CAGTACCGGC AAGCCGCGAG CAGCCCCCGC CCGTTGCCAG GGTCCCGGAC 
ACCGCTGGCG AGAGATCGTC GCCCCGGAGC CCCCAGCCGA GCAAGGAGCG CCTGCGCTTC 
CAGTTCTTAG AGCAGAAGTA CGGCTACTAT CACTGCAAGG ACTGCAATAT CCGCTGGGAA 
AGTGCCTATG TGTGGTGTGT ACAGGGCACT AACAAGGTTT ACTACAAGCA GTTTTGCCGA 
ACATGCCAGA AGTCTTATAA CCCTTACCGA GTGGAGGATA TCACCTGCCA AATTATGCTG 
GTGGATAAGC AGTTCTCAGA AACCAAAAGG AGGGATGGAT GCTCGCTCTG GCTCTGGATT 
GGTTGTAGAC ACAGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZAR1 ( XM_024993376.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849144.1 Bos taurus zygote arrest 1 (ZAR1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235417
Clone ID Related Accession (Same CDS sequence)	XM_024993376.1
Accession Version	XM_024993376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	zygote arrest protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC TGGGGGACGT CGTCCTGGAT GGTTACCTGT ACCCGGCGTG CGCTCTCTAC 
TCGTACCGGT GCCTCTACCC GGCCGCCGCC GCCGCCAAGG GAAAAAGCGG GGCGGACGAG 
GGTGGCTGGC GACCCCGGGG CGGGGGCTAC CCTCCCGTTT CCTCCTCCTC TGACGGCGCG 
GCCTCGTCGT CGTTCCCGGG CCACGGGCAG CTGGCGGCCG CCGAGTACGT CCACAGCTAC 
CAGCGCGCGC AGCTCATGGC CCTGCTGTCG CAGGTGGGCC CCCGCCCGGC CAGCACTCGG 
GACGCGGCGG TGCAGGTGAA CCCGTTCCGC GACGTATCGG TGCAGTGCTC GCTGGGGCGG 
CGGACGCTGG GGCACAGGGC CCGCGAGTCC GGCCCGAGTC CGGATCCCGA GGGCGCGGCG 
GACGCGGGCG GCAGCTGCCC GGCCTCCCCG CAGCGCGCCC GCCGGGGCCC GGAGCAGGAC 
AGCCCGCCGA GCCGCGCCCC GCGGCGCGTG CGTTTCCTGC GCACCCTGGC TGTGTACTCG 
CCCGTGACCT CCCGCTGCCT AGCCACCCTC CTGGAGGGGG CCGAGGCCGT GGCGGGCCAG 
CAGAGGCCCG GGGAGCCGGA GACTGAGCGA GGGCCGCCAC CTGCGAGGCC CCGAGGCCCC 
GAGGAGGGAG ACGGGTCGGC GAGGAAGGTT TCCCTGCAGC TGCAGCCTGA GGAGGACGAG 
GCCCAGGCCG CAGTACCGGC AAGCCGCGAG CAGCCCCCGC CCGTTGCCAG GGTCCCGGAC 
ACCGCTGGCG AGAGATCGTC GCCCCGGAGC CCCCAGCCGA GCAAGGAGCG CCTGCGCTTC 
CAGTTCTTAG AGCAGAAGTA CGGCTACTAT CACTGCAAGG ACTGCAATAT CCGCTGGGAA 
AGTGCCTATG TGTGGTGTGT ACAGGGCACT AACAAGATTA TGCTGGTGGA TAAGCAGTTC 
TCAGAAACCA AAAGGAGGGA TGGATGCTCG CTCTGGCTCT GGATTGGTTG TAGACACAGA 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849144.1
CDS	39..1064
Translation	MAALGDVVLDGYLYPACALYSYRCLYPAAAAAKGKSGADEGGWRPRGGGYPPVSSSSDGAASSSFPGHGQLAAAEYVHSYQRAQLMALLSQVGPRPASTRDAAVQVNPFRDVSVQCSLGRRTLGHRARESGPSPDPEGAADAGGSCPASPQRARRGPEQDSPPSRAPRRVRFLRTLAVYSPVTSRCLATLLEGAEAVAGQQRPGEPETERGPPPARPRGPEEGDGSARKVSLQLQPEEDEAQAAVPASREQPPPVARVPDTAGERSSPRSPQPSKERLRFQFLEQKYGYYHCKDCNIRWESAYVWCVQGTNKIMLVDKQFSETKRRDGCSLWLWIGCRHRL

Target ORF information:

RefSeq Version	XM_024993376.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zygote arrest 1 (ZAR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993376.1

ORF Insert Sequence:

ATGGCTGCCC TGGGGGACGT CGTCCTGGAT GGTTACCTGT ACCCGGCGTG CGCTCTCTAC 
TCGTACCGGT GCCTCTACCC GGCCGCCGCC GCCGCCAAGG GAAAAAGCGG GGCGGACGAG 
GGTGGCTGGC GACCCCGGGG CGGGGGCTAC CCTCCCGTTT CCTCCTCCTC TGACGGCGCG 
GCCTCGTCGT CGTTCCCGGG CCACGGGCAG CTGGCGGCCG CCGAGTACGT CCACAGCTAC 
CAGCGCGCGC AGCTCATGGC CCTGCTGTCG CAGGTGGGCC CCCGCCCGGC CAGCACTCGG 
GACGCGGCGG TGCAGGTGAA CCCGTTCCGC GACGTATCGG TGCAGTGCTC GCTGGGGCGG 
CGGACGCTGG GGCACAGGGC CCGCGAGTCC GGCCCGAGTC CGGATCCCGA GGGCGCGGCG 
GACGCGGGCG GCAGCTGCCC GGCCTCCCCG CAGCGCGCCC GCCGGGGCCC GGAGCAGGAC 
AGCCCGCCGA GCCGCGCCCC GCGGCGCGTG CGTTTCCTGC GCACCCTGGC TGTGTACTCG 
CCCGTGACCT CCCGCTGCCT AGCCACCCTC CTGGAGGGGG CCGAGGCCGT GGCGGGCCAG 
CAGAGGCCCG GGGAGCCGGA GACTGAGCGA GGGCCGCCAC CTGCGAGGCC CCGAGGCCCC 
GAGGAGGGAG ACGGGTCGGC GAGGAAGGTT TCCCTGCAGC TGCAGCCTGA GGAGGACGAG 
GCCCAGGCCG CAGTACCGGC AAGCCGCGAG CAGCCCCCGC CCGTTGCCAG GGTCCCGGAC 
ACCGCTGGCG AGAGATCGTC GCCCCGGAGC CCCCAGCCGA GCAAGGAGCG CCTGCGCTTC 
CAGTTCTTAG AGCAGAAGTA CGGCTACTAT CACTGCAAGG ACTGCAATAT CCGCTGGGAA 
AGTGCCTATG TGTGGTGTGT ACAGGGCACT AACAAGATTA TGCTGGTGGA TAAGCAGTTC 
TCAGAAACCA AAAGGAGGGA TGGATGCTCG CTCTGGCTCT GGATTGGTTG TAGACACAGA 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Zygote arrest 1 gene in pig, cattle and human: evidence of different transcript variants in male and female germ cells.
Reproductive biology and endocrinology : RB&E412(2006 Mar)
Uzbekova S,Roy-Sabau M,Dalbiès-Tran R,Perreau C,Papillier P,Mompart F,Thelie A,Pennetier S,Cognie J,Cadoret V,Royere D,Monget P,Mermillod P

Expression pattern of the maternal factor zygote arrest 1 (Zar1) in bovine tissues, oocytes, and embryos.
Molecular reproduction and development69(4)375-80(2004 Dec)
Brevini TA,Cillo F,Colleoni S,Lazzari G,Galli C,Gandolfi F

Spatio-temporal expression of the germ cell marker genes MATER, ZAR1, GDF9, BMP15,andVASA in adult bovine tissues, oocytes, and preimplantation embryos.
Biology of reproduction71(4)1359-66(2004 Oct)
Pennetier S,Uzbekova S,Perreau C,Papillier P,Mermillod P,Dalbiès-Tran R

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ZAR1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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