DTL cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DTL
Entrez Gene ID	539953
Full Name	denticleless E3 ubiquitin protein ligase homolog
General protein information	Preferred Names denticleless E3 ubiquitin protein ligase homolog Names denticleless protein homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003587108.4	XP_003587156.1	denticleless protein homolog
XM_024976881.1	XP_024832649.1	denticleless protein homolog
XM_003587108.5	XP_003587156.1	denticleless protein homolog
XM_003583269.4	XP_003583317.2	denticleless protein homolog

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	DTL	XP_001108416.1
Mus musculus (house mouse)	Dtl	NP_084042.1
Homo sapiens (human)	DTL	NP_057532.3
Bos taurus (cattle)	DTL	XP_003587156.1
Danio rerio (zebrafish)	dtl	NP_775348.1
Rattus norvegicus (Norway rat)	Dtl	XP_223074.5
Xenopus tropicalis (tropical clawed frog)	dtl	NP_989402.1
Canis lupus familiaris (dog)	DTL	XP_547399.2
Pan troglodytes (chimpanzee)	DTL	XP_525058.3
Gallus gallus (chicken)	DTL	NP_001026219.1

Gene Ontology

The following DTL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DTL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa27550	XM_003587108.5 Latest version!	Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa27550	XM_003583269.4 Latest version!	Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa27550	XM_024976881.1 Latest version!	Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBa27550	XM_003587108.4	Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DTL ( XM_003587108.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587156.1 Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), mRNA.

Price & Availability↑

CloneID	OBa27550
Clone ID Related Accession (Same CDS sequence)	XM_003587108.4 , XM_024976881.1 , XM_003587108.5 , XM_003583269.4
Accession Version	XM_003587108.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	denticleless protein homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587108.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTCA ATTCGGTGCT CCGCCAGCCC CAGTTTGGCG TCCCGAGAAA TGGATGGTCT 
TCACAGTACC CACTACAGTC CCTGCTCACT GGCTATCAGT GCAACTATAA TGACGAGCAT 
ACTTCTTATG GAGAAACAGG AACCCCAGTT CCTCCTTTTG GATGCACCTT CTCTTCTGCT 
CCCAGTATGG AGCATATACT AGCAGTTGCC AATGAAGAAG GCTTTGTTAG GTTATATAAC 
ACAGAATCAC ACACCAGTAG AAAGAAGTGC ATCAAAGAAT GGATGGCTCA CTGGAATGCT 
GTTTTTGACC TGGCCTGGGT CCCTGGTGAA TTTAAGCTTG TTACAGCAGC AGGTGATCAG 
ACAGCCAAAT TTTGGGATGT AAATGCTGGT GAGCTGATTG GAACTTGCAA AGGTCATCAG 
TGTAGCCTCA AGTCAGTTGC CTTTTCTAAG TTTGAGAAAG CTGTCTTCTG TACAGGGGGT 
AGAGATGGCA ACATTATGGT CTGGGATACC AGGTGCAACA AAAAAGATGG ATTTTACAGG 
CAAGTGAACC AAATCAGTGG AGCTCATAAT ACCTTAGACA AGCAAACCCC TTCAAAACCT 
AAGAAGAAAC AGAATTCCAA AGGGCTTGCT CCTTCTGTGG ATTTCCAGCA GAGTGTTACT 
GTGGTCCTCT TTCAGGATGA GAACACATTA GTCTCAGCAG GAGCTGTGGA TGGCATAATC 
AAAATATGGG ATTTACGTAA GAATTATACT GCTTATCGAC AGGAACCCAT AGCATCCAAG 
TCTTTCCTGT ACCCAGGTAG CAGCACTCGA AAACTAGGAT ACTCAAGTCT GATTTTGGAT 
TCTACTGGCT CCACTTTATT TGCTAACTGC ACAGATGACA ACATCTACAT GTTCAATATG 
ACTGGATTAA AGACTTCTCC AGTGGCTGTC TTCAGTGGAC ATCAGAACTC TACCTTTTAT 
GTTAAATCCA GTATTAGTCC AGATGACCAG TTTTTAGTCA GTGGCTCAAG TGATGAAGCT 
GCCTACATCT GGAAGGTCTC CATGCCCTGG CATCCTCCCA CTGTGCTCCT GGGTCATTCT 
CAAGAGGTCA CATCTGTGTG CTGGTGTCCA TCAGACTTCA CAAAGATTGC TACTTGCTCT 
GATGACAATA CACTGAAAGT CTGGCGCTTG AACAGAGATT TAGAGGAGAA ATCAGGAGGA 
GATAAACTCT CCATAGTGGG ATGGGCCTCT CAGAAGAAGA GAGAGGTGAA AGCCGACCTT 
GTGGCAGTGC CCAGCAGCCA GAGCACTCCG GCCAAAGCAC CCAGAGCAAA GAGCAGTCCG 
TGCACCTCCT CCCCGTCGTC GGCAGCCTGT GCTCCGAGCT GCGCCGGGGA CCTGCCCCTC 
CCTTCAAATA CACCTACCTT CTCTCTTAAG ACCCCTCCTG CCAAGGCCCG GTCTCCCATC 
AGCAGAAGAG GCTCTGTGTC CTCCGTCTCT CCCAAGCCAC CCTCATCTTT CAAGATGTCC 
ATTAGAAACT GGATGACCCG AACGGCTTCC TTATCACCAC CCGTCACTCC ACCTGCTTCC 
GAAACCAAGA TCACGTCTCC CAGAAAAGCC CTCATACCTG TGAGCCTGAA ATCATCCCAG 
GCAGTGGCTT GCTCTGAGTC TAGAAATAGA GTGAAGAGGA GGCTAGACTC CAGTTGTCTG 
GAGAATGTGA AACAAAAGTG TGTAAAGAGT TGCAGCTGTG TGACTGAGCT TGATGACCCC 
GTTGAAAAGC TTCATTTGGA TCTGTGCTGC CTTGCTGGTG CCCAGGAAGA CCTTGGGAAG 
GACTCGCTAG GTCCTACCAA ACCAAGCAAG ATCGAAGGAG CTAGTATGAG TGTCTCTGAG 
CCTCCTTCTC CTGCCAGTCC TTATGCTTCG GAAGGCTGTG GAACGCTACC TCTTCCTTTG 
GGATCTTGTG GAGAAGGCTC TGAAGTGGTA GGCAAAGAGA ATAGCTCCCC AGAGAATAAA 
AACTGGTTGT TGGCCATGGC AGCCAAACGG AAGGCGGAGA ATTCATCTCC ACGAAGTCCA 
TCGTCCCAGA CCCCCAATCC CAGGAGACAG AGCGGAAAGA CTTCTCCAGG CCCGGTCACC 
ATTACTCCCA GCTCCATGCG GAAAATCTGT ACATACTTCC ATAGAAAGTC CCAAGATGAC 
TTCTGTAGTC CAGAACAGTC AACAGAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587156.1
CDS	125..2317
Translation	MLFNSVLRQPQFGVPRNGWSSQYPLQSLLTGYQCNYNDEHTSYGETGTPVPPFGCTFSSAPSMEHILAVANEEGFVRLYNTESHTSRKKCIKEWMAHWNAVFDLAWVPGEFKLVTAAGDQTAKFWDVNAGELIGTCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMVWDTRCNKKDGFYRQVNQISGAHNTLDKQTPSKPKKKQNSKGLAPSVDFQQSVTVVLFQDENTLVSAGAVDGIIKIWDLRKNYTAYRQEPIASKSFLYPGSSTRKLGYSSLILDSTGSTLFANCTDDNIYMFNMTGLKTSPVAVFSGHQNSTFYVKSSISPDDQFLVSGSSDEAAYIWKVSMPWHPPTVLLGHSQEVTSVCWCPSDFTKIATCSDDNTLKVWRLNRDLEEKSGGDKLSIVGWASQKKREVKADLVAVPSSQSTPAKAPRAKSSPCTSSPSSAACAPSCAGDLPLPSNTPTFSLKTPPAKARSPISRRGSVSSVSPKPPSSFKMSIRNWMTRTASLSPPVTPPASETKITSPRKALIPVSLKSSQAVACSESRNRVKRRLDSSCLENVKQKCVKSCSCVTELDDPVEKLHLDLCCLAGAQEDLGKDSLGPTKPSKIEGASMSVSEPPSPASPYASEGCGTLPLPLGSCGEGSEVVGKENSSPENKNWLLAMAAKRKAENSSPRSPSSQTPNPRRQSGKTSPGPVTITPSSMRKICTYFHRKSQDDFCSPEQSTEL

Target ORF information:

RefSeq Version	XM_003587108.4
Organism	Bos taurus(cattle)
Definition	Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587108.4

ORF Insert Sequence:

ATGCTCTTCA ATTCGGTGCT CCGCCAGCCC CAGTTTGGCG TCCCGAGAAA TGGATGGTCT 
TCACAGTACC CACTACAGTC CCTGCTCACT GGCTATCAGT GCAACTATAA TGACGAGCAT 
ACTTCTTATG GAGAAACAGG AACCCCAGTT CCTCCTTTTG GATGCACCTT CTCTTCTGCT 
CCCAGTATGG AGCATATACT AGCAGTTGCC AATGAAGAAG GCTTTGTTAG GTTATATAAC 
ACAGAATCAC ACACCAGTAG AAAGAAGTGC ATCAAAGAAT GGATGGCTCA CTGGAATGCT 
GTTTTTGACC TGGCCTGGGT CCCTGGTGAA TTTAAGCTTG TTACAGCAGC AGGTGATCAG 
ACAGCCAAAT TTTGGGATGT AAATGCTGGT GAGCTGATTG GAACTTGCAA AGGTCATCAG 
TGTAGCCTCA AGTCAGTTGC CTTTTCTAAG TTTGAGAAAG CTGTCTTCTG TACAGGGGGT 
AGAGATGGCA ACATTATGGT CTGGGATACC AGGTGCAACA AAAAAGATGG ATTTTACAGG 
CAAGTGAACC AAATCAGTGG AGCTCATAAT ACCTTAGACA AGCAAACCCC TTCAAAACCT 
AAGAAGAAAC AGAATTCCAA AGGGCTTGCT CCTTCTGTGG ATTTCCAGCA GAGTGTTACT 
GTGGTCCTCT TTCAGGATGA GAACACATTA GTCTCAGCAG GAGCTGTGGA TGGCATAATC 
AAAATATGGG ATTTACGTAA GAATTATACT GCTTATCGAC AGGAACCCAT AGCATCCAAG 
TCTTTCCTGT ACCCAGGTAG CAGCACTCGA AAACTAGGAT ACTCAAGTCT GATTTTGGAT 
TCTACTGGCT CCACTTTATT TGCTAACTGC ACAGATGACA ACATCTACAT GTTCAATATG 
ACTGGATTAA AGACTTCTCC AGTGGCTGTC TTCAGTGGAC ATCAGAACTC TACCTTTTAT 
GTTAAATCCA GTATTAGTCC AGATGACCAG TTTTTAGTCA GTGGCTCAAG TGATGAAGCT 
GCCTACATCT GGAAGGTCTC CATGCCCTGG CATCCTCCCA CTGTGCTCCT GGGTCATTCT 
CAAGAGGTCA CATCTGTGTG CTGGTGTCCA TCAGACTTCA CAAAGATTGC TACTTGCTCT 
GATGACAATA CACTGAAAGT CTGGCGCTTG AACAGAGATT TAGAGGAGAA ATCAGGAGGA 
GATAAACTCT CCATAGTGGG ATGGGCCTCT CAGAAGAAGA GAGAGGTGAA AGCCGACCTT 
GTGGCAGTGC CCAGCAGCCA GAGCACTCCG GCCAAAGCAC CCAGAGCAAA GAGCAGTCCG 
TGCACCTCCT CCCCGTCGTC GGCAGCCTGT GCTCCGAGCT GCGCCGGGGA CCTGCCCCTC 
CCTTCAAATA CACCTACCTT CTCTCTTAAG ACCCCTCCTG CCAAGGCCCG GTCTCCCATC 
AGCAGAAGAG GCTCTGTGTC CTCCGTCTCT CCCAAGCCAC CCTCATCTTT CAAGATGTCC 
ATTAGAAACT GGATGACCCG AACGGCTTCC TTATCACCAC CCGTCACTCC ACCTGCTTCC 
GAAACCAAGA TCACGTCTCC CAGAAAAGCC CTCATACCTG TGAGCCTGAA ATCATCCCAG 
GCAGTGGCTT GCTCTGAGTC TAGAAATAGA GTGAAGAGGA GGCTAGACTC CAGTTGTCTG 
GAGAATGTGA AACAAAAGTG TGTAAAGAGT TGCAGCTGTG TGACTGAGCT TGATGACCCC 
GTTGAAAAGC TTCATTTGGA TCTGTGCTGC CTTGCTGGTG CCCAGGAAGA CCTTGGGAAG 
GACTCGCTAG GTCCTACCAA ACCAAGCAAG ATCGAAGGAG CTAGTATGAG TGTCTCTGAG 
CCTCCTTCTC CTGCCAGTCC TTATGCTTCG GAAGGCTGTG GAACGCTACC TCTTCCTTTG 
GGATCTTGTG GAGAAGGCTC TGAAGTGGTA GGCAAAGAGA ATAGCTCCCC AGAGAATAAA 
AACTGGTTGT TGGCCATGGC AGCCAAACGG AAGGCGGAGA ATTCATCTCC ACGAAGTCCA 
TCGTCCCAGA CCCCCAATCC CAGGAGACAG AGCGGAAAGA CTTCTCCAGG CCCGGTCACC 
ATTACTCCCA GCTCCATGCG GAAAATCTGT ACATACTTCC ATAGAAAGTC CCAAGATGAC 
TTCTGTAGTC CAGAACAGTC AACAGAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DTL ( XM_024976881.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832649.1 Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa27550
Clone ID Related Accession (Same CDS sequence)	XM_003587108.4 , XM_024976881.1 , XM_003587108.5 , XM_003583269.4
Accession Version	XM_024976881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	denticleless protein homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTCA ATTCGGTGCT CCGCCAGCCC CAGTTTGGCG TCCCGAGAAA TGGATGGTCT 
TCACAGTACC CACTACAGTC CCTGCTCACT GGCTATCAGT GCAACTATAA TGACGAGCAT 
ACTTCTTATG GAGAAACAGG AACCCCAGTT CCTCCTTTTG GATGCACCTT CTCTTCTGCT 
CCCAGTATGG AGCATATACT AGCAGTTGCC AATGAAGAAG GCTTTGTTAG GTTATATAAC 
ACAGAATCAC ACACCAGTAG AAAGAAGTGC ATCAAAGAAT GGATGGCTCA CTGGAATGCT 
GTTTTTGACC TGGCCTGGGT CCCTGGTGAA TTTAAGCTTG TTACAGCAGC AGGTGATCAG 
ACAGCCAAAT TTTGGGATGT AAATGCTGGT GAGCTGATTG GAACTTGCAA AGGTCATCAG 
TGTAGCCTCA AGTCAGTTGC CTTTTCTAAG TTTGAGAAAG CTGTCTTCTG TACAGGGGGT 
AGAGATGGCA ACATTATGGT CTGGGATACC AGGTGCAACA AAAAAGATGG ATTTTACAGG 
CAAGTGAACC AAATCAGTGG AGCTCATAAT ACCTTAGACA AGCAAACCCC TTCAAAACCT 
AAGAAGAAAC AGAATTCCAA AGGGCTTGCT CCTTCTGTGG ATTTCCAGCA GAGTGTTACT 
GTGGTCCTCT TTCAGGATGA GAACACATTA GTCTCAGCAG GAGCTGTGGA TGGCATAATC 
AAAATATGGG ATTTACGTAA GAATTATACT GCTTATCGAC AGGAACCCAT AGCATCCAAG 
TCTTTCCTGT ACCCAGGTAG CAGCACTCGA AAACTAGGAT ACTCAAGTCT GATTTTGGAT 
TCTACTGGCT CCACTTTATT TGCTAACTGC ACAGATGACA ACATCTACAT GTTCAATATG 
ACTGGATTAA AGACTTCTCC AGTGGCTGTC TTCAGTGGAC ATCAGAACTC TACCTTTTAT 
GTTAAATCCA GTATTAGTCC AGATGACCAG TTTTTAGTCA GTGGCTCAAG TGATGAAGCT 
GCCTACATCT GGAAGGTCTC CATGCCCTGG CATCCTCCCA CTGTGCTCCT GGGTCATTCT 
CAAGAGGTCA CATCTGTGTG CTGGTGTCCA TCAGACTTCA CAAAGATTGC TACTTGCTCT 
GATGACAATA CACTGAAAGT CTGGCGCTTG AACAGAGATT TAGAGGAGAA ATCAGGAGGA 
GATAAACTCT CCATAGTGGG ATGGGCCTCT CAGAAGAAGA GAGAGGTGAA AGCCGACCTT 
GTGGCAGTGC CCAGCAGCCA GAGCACTCCG GCCAAAGCAC CCAGAGCAAA GAGCAGTCCG 
TGCACCTCCT CCCCGTCGTC GGCAGCCTGT GCTCCGAGCT GCGCCGGGGA CCTGCCCCTC 
CCTTCAAATA CACCTACCTT CTCTCTTAAG ACCCCTCCTG CCAAGGCCCG GTCTCCCATC 
AGCAGAAGAG GCTCTGTGTC CTCCGTCTCT CCCAAGCCAC CCTCATCTTT CAAGATGTCC 
ATTAGAAACT GGATGACCCG AACGGCTTCC TTATCACCAC CCGTCACTCC ACCTGCTTCC 
GAAACCAAGA TCACGTCTCC CAGAAAAGCC CTCATACCTG TGAGCCTGAA ATCATCCCAG 
GCAGTGGCTT GCTCTGAGTC TAGAAATAGA GTGAAGAGGA GGCTAGACTC CAGTTGTCTG 
GAGAATGTGA AACAAAAGTG TGTAAAGAGT TGCAGCTGTG TGACTGAGCT TGATGACCCC 
GTTGAAAAGC TTCATTTGGA TCTGTGCTGC CTTGCTGGTG CCCAGGAAGA CCTTGGGAAG 
GACTCGCTAG GTCCTACCAA ACCAAGCAAG ATCGAAGGAG CTAGTATGAG TGTCTCTGAG 
CCTCCTTCTC CTGCCAGTCC TTATGCTTCG GAAGGCTGTG GAACGCTACC TCTTCCTTTG 
GGATCTTGTG GAGAAGGCTC TGAAGTGGTA GGCAAAGAGA ATAGCTCCCC AGAGAATAAA 
AACTGGTTGT TGGCCATGGC AGCCAAACGG AAGGCGGAGA ATTCATCTCC ACGAAGTCCA 
TCGTCCCAGA CCCCCAATCC CAGGAGACAG AGCGGAAAGA CTTCTCCAGG CCCGGTCACC 
ATTACTCCCA GCTCCATGCG GAAAATCTGT ACATACTTCC ATAGAAAGTC CCAAGATGAC 
TTCTGTAGTC CAGAACAGTC AACAGAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832649.1
CDS	157..2349
Translation	MLFNSVLRQPQFGVPRNGWSSQYPLQSLLTGYQCNYNDEHTSYGETGTPVPPFGCTFSSAPSMEHILAVANEEGFVRLYNTESHTSRKKCIKEWMAHWNAVFDLAWVPGEFKLVTAAGDQTAKFWDVNAGELIGTCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMVWDTRCNKKDGFYRQVNQISGAHNTLDKQTPSKPKKKQNSKGLAPSVDFQQSVTVVLFQDENTLVSAGAVDGIIKIWDLRKNYTAYRQEPIASKSFLYPGSSTRKLGYSSLILDSTGSTLFANCTDDNIYMFNMTGLKTSPVAVFSGHQNSTFYVKSSISPDDQFLVSGSSDEAAYIWKVSMPWHPPTVLLGHSQEVTSVCWCPSDFTKIATCSDDNTLKVWRLNRDLEEKSGGDKLSIVGWASQKKREVKADLVAVPSSQSTPAKAPRAKSSPCTSSPSSAACAPSCAGDLPLPSNTPTFSLKTPPAKARSPISRRGSVSSVSPKPPSSFKMSIRNWMTRTASLSPPVTPPASETKITSPRKALIPVSLKSSQAVACSESRNRVKRRLDSSCLENVKQKCVKSCSCVTELDDPVEKLHLDLCCLAGAQEDLGKDSLGPTKPSKIEGASMSVSEPPSPASPYASEGCGTLPLPLGSCGEGSEVVGKENSSPENKNWLLAMAAKRKAENSSPRSPSSQTPNPRRQSGKTSPGPVTITPSSMRKICTYFHRKSQDDFCSPEQSTEL

Target ORF information:

RefSeq Version	XM_024976881.1
Organism	Bos taurus(cattle)
Definition	Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976881.1

ORF Insert Sequence:

ATGCTCTTCA ATTCGGTGCT CCGCCAGCCC CAGTTTGGCG TCCCGAGAAA TGGATGGTCT 
TCACAGTACC CACTACAGTC CCTGCTCACT GGCTATCAGT GCAACTATAA TGACGAGCAT 
ACTTCTTATG GAGAAACAGG AACCCCAGTT CCTCCTTTTG GATGCACCTT CTCTTCTGCT 
CCCAGTATGG AGCATATACT AGCAGTTGCC AATGAAGAAG GCTTTGTTAG GTTATATAAC 
ACAGAATCAC ACACCAGTAG AAAGAAGTGC ATCAAAGAAT GGATGGCTCA CTGGAATGCT 
GTTTTTGACC TGGCCTGGGT CCCTGGTGAA TTTAAGCTTG TTACAGCAGC AGGTGATCAG 
ACAGCCAAAT TTTGGGATGT AAATGCTGGT GAGCTGATTG GAACTTGCAA AGGTCATCAG 
TGTAGCCTCA AGTCAGTTGC CTTTTCTAAG TTTGAGAAAG CTGTCTTCTG TACAGGGGGT 
AGAGATGGCA ACATTATGGT CTGGGATACC AGGTGCAACA AAAAAGATGG ATTTTACAGG 
CAAGTGAACC AAATCAGTGG AGCTCATAAT ACCTTAGACA AGCAAACCCC TTCAAAACCT 
AAGAAGAAAC AGAATTCCAA AGGGCTTGCT CCTTCTGTGG ATTTCCAGCA GAGTGTTACT 
GTGGTCCTCT TTCAGGATGA GAACACATTA GTCTCAGCAG GAGCTGTGGA TGGCATAATC 
AAAATATGGG ATTTACGTAA GAATTATACT GCTTATCGAC AGGAACCCAT AGCATCCAAG 
TCTTTCCTGT ACCCAGGTAG CAGCACTCGA AAACTAGGAT ACTCAAGTCT GATTTTGGAT 
TCTACTGGCT CCACTTTATT TGCTAACTGC ACAGATGACA ACATCTACAT GTTCAATATG 
ACTGGATTAA AGACTTCTCC AGTGGCTGTC TTCAGTGGAC ATCAGAACTC TACCTTTTAT 
GTTAAATCCA GTATTAGTCC AGATGACCAG TTTTTAGTCA GTGGCTCAAG TGATGAAGCT 
GCCTACATCT GGAAGGTCTC CATGCCCTGG CATCCTCCCA CTGTGCTCCT GGGTCATTCT 
CAAGAGGTCA CATCTGTGTG CTGGTGTCCA TCAGACTTCA CAAAGATTGC TACTTGCTCT 
GATGACAATA CACTGAAAGT CTGGCGCTTG AACAGAGATT TAGAGGAGAA ATCAGGAGGA 
GATAAACTCT CCATAGTGGG ATGGGCCTCT CAGAAGAAGA GAGAGGTGAA AGCCGACCTT 
GTGGCAGTGC CCAGCAGCCA GAGCACTCCG GCCAAAGCAC CCAGAGCAAA GAGCAGTCCG 
TGCACCTCCT CCCCGTCGTC GGCAGCCTGT GCTCCGAGCT GCGCCGGGGA CCTGCCCCTC 
CCTTCAAATA CACCTACCTT CTCTCTTAAG ACCCCTCCTG CCAAGGCCCG GTCTCCCATC 
AGCAGAAGAG GCTCTGTGTC CTCCGTCTCT CCCAAGCCAC CCTCATCTTT CAAGATGTCC 
ATTAGAAACT GGATGACCCG AACGGCTTCC TTATCACCAC CCGTCACTCC ACCTGCTTCC 
GAAACCAAGA TCACGTCTCC CAGAAAAGCC CTCATACCTG TGAGCCTGAA ATCATCCCAG 
GCAGTGGCTT GCTCTGAGTC TAGAAATAGA GTGAAGAGGA GGCTAGACTC CAGTTGTCTG 
GAGAATGTGA AACAAAAGTG TGTAAAGAGT TGCAGCTGTG TGACTGAGCT TGATGACCCC 
GTTGAAAAGC TTCATTTGGA TCTGTGCTGC CTTGCTGGTG CCCAGGAAGA CCTTGGGAAG 
GACTCGCTAG GTCCTACCAA ACCAAGCAAG ATCGAAGGAG CTAGTATGAG TGTCTCTGAG 
CCTCCTTCTC CTGCCAGTCC TTATGCTTCG GAAGGCTGTG GAACGCTACC TCTTCCTTTG 
GGATCTTGTG GAGAAGGCTC TGAAGTGGTA GGCAAAGAGA ATAGCTCCCC AGAGAATAAA 
AACTGGTTGT TGGCCATGGC AGCCAAACGG AAGGCGGAGA ATTCATCTCC ACGAAGTCCA 
TCGTCCCAGA CCCCCAATCC CAGGAGACAG AGCGGAAAGA CTTCTCCAGG CCCGGTCACC 
ATTACTCCCA GCTCCATGCG GAAAATCTGT ACATACTTCC ATAGAAAGTC CCAAGATGAC 
TTCTGTAGTC CAGAACAGTC AACAGAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DTL ( XM_003587108.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587156.1 Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa27550
Clone ID Related Accession (Same CDS sequence)	XM_003587108.4 , XM_024976881.1 , XM_003587108.5 , XM_003583269.4
Accession Version	XM_003587108.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	denticleless protein homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587108.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTCA ATTCGGTGCT CCGCCAGCCC CAGTTTGGCG TCCCGAGAAA TGGATGGTCT 
TCACAGTACC CACTACAGTC CCTGCTCACT GGCTATCAGT GCAACTATAA TGACGAGCAT 
ACTTCTTATG GAGAAACAGG AACCCCAGTT CCTCCTTTTG GATGCACCTT CTCTTCTGCT 
CCCAGTATGG AGCATATACT AGCAGTTGCC AATGAAGAAG GCTTTGTTAG GTTATATAAC 
ACAGAATCAC ACACCAGTAG AAAGAAGTGC ATCAAAGAAT GGATGGCTCA CTGGAATGCT 
GTTTTTGACC TGGCCTGGGT CCCTGGTGAA TTTAAGCTTG TTACAGCAGC AGGTGATCAG 
ACAGCCAAAT TTTGGGATGT AAATGCTGGT GAGCTGATTG GAACTTGCAA AGGTCATCAG 
TGTAGCCTCA AGTCAGTTGC CTTTTCTAAG TTTGAGAAAG CTGTCTTCTG TACAGGGGGT 
AGAGATGGCA ACATTATGGT CTGGGATACC AGGTGCAACA AAAAAGATGG ATTTTACAGG 
CAAGTGAACC AAATCAGTGG AGCTCATAAT ACCTTAGACA AGCAAACCCC TTCAAAACCT 
AAGAAGAAAC AGAATTCCAA AGGGCTTGCT CCTTCTGTGG ATTTCCAGCA GAGTGTTACT 
GTGGTCCTCT TTCAGGATGA GAACACATTA GTCTCAGCAG GAGCTGTGGA TGGCATAATC 
AAAATATGGG ATTTACGTAA GAATTATACT GCTTATCGAC AGGAACCCAT AGCATCCAAG 
TCTTTCCTGT ACCCAGGTAG CAGCACTCGA AAACTAGGAT ACTCAAGTCT GATTTTGGAT 
TCTACTGGCT CCACTTTATT TGCTAACTGC ACAGATGACA ACATCTACAT GTTCAATATG 
ACTGGATTAA AGACTTCTCC AGTGGCTGTC TTCAGTGGAC ATCAGAACTC TACCTTTTAT 
GTTAAATCCA GTATTAGTCC AGATGACCAG TTTTTAGTCA GTGGCTCAAG TGATGAAGCT 
GCCTACATCT GGAAGGTCTC CATGCCCTGG CATCCTCCCA CTGTGCTCCT GGGTCATTCT 
CAAGAGGTCA CATCTGTGTG CTGGTGTCCA TCAGACTTCA CAAAGATTGC TACTTGCTCT 
GATGACAATA CACTGAAAGT CTGGCGCTTG AACAGAGATT TAGAGGAGAA ATCAGGAGGA 
GATAAACTCT CCATAGTGGG ATGGGCCTCT CAGAAGAAGA GAGAGGTGAA AGCCGACCTT 
GTGGCAGTGC CCAGCAGCCA GAGCACTCCG GCCAAAGCAC CCAGAGCAAA GAGCAGTCCG 
TGCACCTCCT CCCCGTCGTC GGCAGCCTGT GCTCCGAGCT GCGCCGGGGA CCTGCCCCTC 
CCTTCAAATA CACCTACCTT CTCTCTTAAG ACCCCTCCTG CCAAGGCCCG GTCTCCCATC 
AGCAGAAGAG GCTCTGTGTC CTCCGTCTCT CCCAAGCCAC CCTCATCTTT CAAGATGTCC 
ATTAGAAACT GGATGACCCG AACGGCTTCC TTATCACCAC CCGTCACTCC ACCTGCTTCC 
GAAACCAAGA TCACGTCTCC CAGAAAAGCC CTCATACCTG TGAGCCTGAA ATCATCCCAG 
GCAGTGGCTT GCTCTGAGTC TAGAAATAGA GTGAAGAGGA GGCTAGACTC CAGTTGTCTG 
GAGAATGTGA AACAAAAGTG TGTAAAGAGT TGCAGCTGTG TGACTGAGCT TGATGACCCC 
GTTGAAAAGC TTCATTTGGA TCTGTGCTGC CTTGCTGGTG CCCAGGAAGA CCTTGGGAAG 
GACTCGCTAG GTCCTACCAA ACCAAGCAAG ATCGAAGGAG CTAGTATGAG TGTCTCTGAG 
CCTCCTTCTC CTGCCAGTCC TTATGCTTCG GAAGGCTGTG GAACGCTACC TCTTCCTTTG 
GGATCTTGTG GAGAAGGCTC TGAAGTGGTA GGCAAAGAGA ATAGCTCCCC AGAGAATAAA 
AACTGGTTGT TGGCCATGGC AGCCAAACGG AAGGCGGAGA ATTCATCTCC ACGAAGTCCA 
TCGTCCCAGA CCCCCAATCC CAGGAGACAG AGCGGAAAGA CTTCTCCAGG CCCGGTCACC 
ATTACTCCCA GCTCCATGCG GAAAATCTGT ACATACTTCC ATAGAAAGTC CCAAGATGAC 
TTCTGTAGTC CAGAACAGTC AACAGAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587156.1
CDS	157..2349
Translation	MLFNSVLRQPQFGVPRNGWSSQYPLQSLLTGYQCNYNDEHTSYGETGTPVPPFGCTFSSAPSMEHILAVANEEGFVRLYNTESHTSRKKCIKEWMAHWNAVFDLAWVPGEFKLVTAAGDQTAKFWDVNAGELIGTCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMVWDTRCNKKDGFYRQVNQISGAHNTLDKQTPSKPKKKQNSKGLAPSVDFQQSVTVVLFQDENTLVSAGAVDGIIKIWDLRKNYTAYRQEPIASKSFLYPGSSTRKLGYSSLILDSTGSTLFANCTDDNIYMFNMTGLKTSPVAVFSGHQNSTFYVKSSISPDDQFLVSGSSDEAAYIWKVSMPWHPPTVLLGHSQEVTSVCWCPSDFTKIATCSDDNTLKVWRLNRDLEEKSGGDKLSIVGWASQKKREVKADLVAVPSSQSTPAKAPRAKSSPCTSSPSSAACAPSCAGDLPLPSNTPTFSLKTPPAKARSPISRRGSVSSVSPKPPSSFKMSIRNWMTRTASLSPPVTPPASETKITSPRKALIPVSLKSSQAVACSESRNRVKRRLDSSCLENVKQKCVKSCSCVTELDDPVEKLHLDLCCLAGAQEDLGKDSLGPTKPSKIEGASMSVSEPPSPASPYASEGCGTLPLPLGSCGEGSEVVGKENSSPENKNWLLAMAAKRKAENSSPRSPSSQTPNPRRQSGKTSPGPVTITPSSMRKICTYFHRKSQDDFCSPEQSTEL

Target ORF information:

RefSeq Version	XM_003587108.5
Organism	Bos taurus(cattle)
Definition	Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587108.5

ORF Insert Sequence:

ATGCTCTTCA ATTCGGTGCT CCGCCAGCCC CAGTTTGGCG TCCCGAGAAA TGGATGGTCT 
TCACAGTACC CACTACAGTC CCTGCTCACT GGCTATCAGT GCAACTATAA TGACGAGCAT 
ACTTCTTATG GAGAAACAGG AACCCCAGTT CCTCCTTTTG GATGCACCTT CTCTTCTGCT 
CCCAGTATGG AGCATATACT AGCAGTTGCC AATGAAGAAG GCTTTGTTAG GTTATATAAC 
ACAGAATCAC ACACCAGTAG AAAGAAGTGC ATCAAAGAAT GGATGGCTCA CTGGAATGCT 
GTTTTTGACC TGGCCTGGGT CCCTGGTGAA TTTAAGCTTG TTACAGCAGC AGGTGATCAG 
ACAGCCAAAT TTTGGGATGT AAATGCTGGT GAGCTGATTG GAACTTGCAA AGGTCATCAG 
TGTAGCCTCA AGTCAGTTGC CTTTTCTAAG TTTGAGAAAG CTGTCTTCTG TACAGGGGGT 
AGAGATGGCA ACATTATGGT CTGGGATACC AGGTGCAACA AAAAAGATGG ATTTTACAGG 
CAAGTGAACC AAATCAGTGG AGCTCATAAT ACCTTAGACA AGCAAACCCC TTCAAAACCT 
AAGAAGAAAC AGAATTCCAA AGGGCTTGCT CCTTCTGTGG ATTTCCAGCA GAGTGTTACT 
GTGGTCCTCT TTCAGGATGA GAACACATTA GTCTCAGCAG GAGCTGTGGA TGGCATAATC 
AAAATATGGG ATTTACGTAA GAATTATACT GCTTATCGAC AGGAACCCAT AGCATCCAAG 
TCTTTCCTGT ACCCAGGTAG CAGCACTCGA AAACTAGGAT ACTCAAGTCT GATTTTGGAT 
TCTACTGGCT CCACTTTATT TGCTAACTGC ACAGATGACA ACATCTACAT GTTCAATATG 
ACTGGATTAA AGACTTCTCC AGTGGCTGTC TTCAGTGGAC ATCAGAACTC TACCTTTTAT 
GTTAAATCCA GTATTAGTCC AGATGACCAG TTTTTAGTCA GTGGCTCAAG TGATGAAGCT 
GCCTACATCT GGAAGGTCTC CATGCCCTGG CATCCTCCCA CTGTGCTCCT GGGTCATTCT 
CAAGAGGTCA CATCTGTGTG CTGGTGTCCA TCAGACTTCA CAAAGATTGC TACTTGCTCT 
GATGACAATA CACTGAAAGT CTGGCGCTTG AACAGAGATT TAGAGGAGAA ATCAGGAGGA 
GATAAACTCT CCATAGTGGG ATGGGCCTCT CAGAAGAAGA GAGAGGTGAA AGCCGACCTT 
GTGGCAGTGC CCAGCAGCCA GAGCACTCCG GCCAAAGCAC CCAGAGCAAA GAGCAGTCCG 
TGCACCTCCT CCCCGTCGTC GGCAGCCTGT GCTCCGAGCT GCGCCGGGGA CCTGCCCCTC 
CCTTCAAATA CACCTACCTT CTCTCTTAAG ACCCCTCCTG CCAAGGCCCG GTCTCCCATC 
AGCAGAAGAG GCTCTGTGTC CTCCGTCTCT CCCAAGCCAC CCTCATCTTT CAAGATGTCC 
ATTAGAAACT GGATGACCCG AACGGCTTCC TTATCACCAC CCGTCACTCC ACCTGCTTCC 
GAAACCAAGA TCACGTCTCC CAGAAAAGCC CTCATACCTG TGAGCCTGAA ATCATCCCAG 
GCAGTGGCTT GCTCTGAGTC TAGAAATAGA GTGAAGAGGA GGCTAGACTC CAGTTGTCTG 
GAGAATGTGA AACAAAAGTG TGTAAAGAGT TGCAGCTGTG TGACTGAGCT TGATGACCCC 
GTTGAAAAGC TTCATTTGGA TCTGTGCTGC CTTGCTGGTG CCCAGGAAGA CCTTGGGAAG 
GACTCGCTAG GTCCTACCAA ACCAAGCAAG ATCGAAGGAG CTAGTATGAG TGTCTCTGAG 
CCTCCTTCTC CTGCCAGTCC TTATGCTTCG GAAGGCTGTG GAACGCTACC TCTTCCTTTG 
GGATCTTGTG GAGAAGGCTC TGAAGTGGTA GGCAAAGAGA ATAGCTCCCC AGAGAATAAA 
AACTGGTTGT TGGCCATGGC AGCCAAACGG AAGGCGGAGA ATTCATCTCC ACGAAGTCCA 
TCGTCCCAGA CCCCCAATCC CAGGAGACAG AGCGGAAAGA CTTCTCCAGG CCCGGTCACC 
ATTACTCCCA GCTCCATGCG GAAAATCTGT ACATACTTCC ATAGAAAGTC CCAAGATGAC 
TTCTGTAGTC CAGAACAGTC AACAGAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DTL ( XM_003583269.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003583317.2 Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), mRNA.

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CloneID	OBa27550
Clone ID Related Accession (Same CDS sequence)	XM_003587108.4 , XM_024976881.1 , XM_003587108.5 , XM_003583269.4
Accession Version	XM_003583269.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	denticleless protein homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583269.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTCA ATTCGGTGCT CCGCCAGCCC CAGTTTGGCG TCCCGAGAAA TGGATGGTCT 
TCACAGTACC CACTACAGTC CCTGCTCACT GGCTATCAGT GCAACTATAA TGACGAGCAT 
ACTTCTTATG GAGAAACAGG AACCCCAGTT CCTCCTTTTG GATGCACCTT CTCTTCTGCT 
CCCAGTATGG AGCATATACT AGCAGTTGCC AATGAAGAAG GCTTTGTTAG GTTATATAAC 
ACAGAATCAC ACACCAGTAG AAAGAAGTGC ATCAAAGAAT GGATGGCTCA CTGGAATGCT 
GTTTTTGACC TGGCCTGGGT CCCTGGTGAA TTTAAGCTTG TTACAGCAGC AGGTGATCAG 
ACAGCCAAAT TTTGGGATGT AAATGCTGGT GAGCTGATTG GAACTTGCAA AGGTCATCAG 
TGTAGCCTCA AGTCAGTTGC CTTTTCTAAG TTTGAGAAAG CTGTCTTCTG TACAGGGGGT 
AGAGATGGCA ACATTATGGT CTGGGATACC AGGTGCAACA AAAAAGATGG ATTTTACAGG 
CAAGTGAACC AAATCAGTGG AGCTCATAAT ACCTTAGACA AGCAAACCCC TTCAAAACCT 
AAGAAGAAAC AGAATTCCAA AGGGCTTGCT CCTTCTGTGG ATTTCCAGCA GAGTGTTACT 
GTGGTCCTCT TTCAGGATGA GAACACATTA GTCTCAGCAG GAGCTGTGGA TGGCATAATC 
AAAATATGGG ATTTACGTAA GAATTATACT GCTTATCGAC AGGAACCCAT AGCATCCAAG 
TCTTTCCTGT ACCCAGGTAG CAGCACTCGA AAACTAGGAT ACTCAAGTCT GATTTTGGAT 
TCTACTGGCT CCACTTTATT TGCTAACTGC ACAGATGACA ACATCTACAT GTTCAATATG 
ACTGGATTAA AGACTTCTCC AGTGGCTGTC TTCAGTGGAC ATCAGAACTC TACCTTTTAT 
GTTAAATCCA GTATTAGTCC AGATGACCAG TTTTTAGTCA GTGGCTCAAG TGATGAAGCT 
GCCTACATCT GGAAGGTCTC CATGCCCTGG CATCCTCCCA CTGTGCTCCT GGGTCATTCT 
CAAGAGGTCA CATCTGTGTG CTGGTGTCCA TCAGACTTCA CAAAGATTGC TACTTGCTCT 
GATGACAATA CACTGAAAGT CTGGCGCTTG AACAGAGATT TAGAGGAGAA ATCAGGAGGA 
GATAAACTCT CCATAGTGGG ATGGGCCTCT CAGAAGAAGA GAGAGGTGAA AGCCGACCTT 
GTGGCAGTGC CCAGCAGCCA GAGCACTCCG GCCAAAGCAC CCAGAGCAAA GAGCAGTCCG 
TGCACCTCCT CCCCGTCGTC GGCAGCCTGT GCTCCGAGCT GCGCCGGGGA CCTGCCCCTC 
CCTTCAAATA CACCTACCTT CTCTCTTAAG ACCCCTCCTG CCAAGGCCCG GTCTCCCATC 
AGCAGAAGAG GCTCTGTGTC CTCCGTCTCT CCCAAGCCAC CCTCATCTTT CAAGATGTCC 
ATTAGAAACT GGATGACCCG AACGGCTTCC TTATCACCAC CCGTCACTCC ACCTGCTTCC 
GAAACCAAGA TCACGTCTCC CAGAAAAGCC CTCATACCTG TGAGCCTGAA ATCATCCCAG 
GCAGTGGCTT GCTCTGAGTC TAGAAATAGA GTGAAGAGGA GGCTAGACTC CAGTTGTCTG 
GAGAATGTGA AACAAAAGTG TGTAAAGAGT TGCAGCTGTG TGACTGAGCT TGATGACCCC 
GTTGAAAAGC TTCATTTGGA TCTGTGCTGC CTTGCTGGTG CCCAGGAAGA CCTTGGGAAG 
GACTCGCTAG GTCCTACCAA ACCAAGCAAG ATCGAAGGAG CTAGTATGAG TGTCTCTGAG 
CCTCCTTCTC CTGCCAGTCC TTATGCTTCG GAAGGCTGTG GAACGCTACC TCTTCCTTTG 
GGATCTTGTG GAGAAGGCTC TGAAGTGGTA GGCAAAGAGA ATAGCTCCCC AGAGAATAAA 
AACTGGTTGT TGGCCATGGC AGCCAAACGG AAGGCGGAGA ATTCATCTCC ACGAAGTCCA 
TCGTCCCAGA CCCCCAATCC CAGGAGACAG AGCGGAAAGA CTTCTCCAGG CCCGGTCACC 
ATTACTCCCA GCTCCATGCG GAAAATCTGT ACATACTTCC ATAGAAAGTC CCAAGATGAC 
TTCTGTAGTC CAGAACAGTC AACAGAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003583317.2
CDS	125..2317
Translation	MLFNSVLRQPQFGVPRNGWSSQYPLQSLLTGYQCNYNDEHTSYGETGTPVPPFGCTFSSAPSMEHILAVANEEGFVRLYNTESHTSRKKCIKEWMAHWNAVFDLAWVPGEFKLVTAAGDQTAKFWDVNAGELIGTCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMVWDTRCNKKDGFYRQVNQISGAHNTLDKQTPSKPKKKQNSKGLAPSVDFQQSVTVVLFQDENTLVSAGAVDGIIKIWDLRKNYTAYRQEPIASKSFLYPGSSTRKLGYSSLILDSTGSTLFANCTDDNIYMFNMTGLKTSPVAVFSGHQNSTFYVKSSISPDDQFLVSGSSDEAAYIWKVSMPWHPPTVLLGHSQEVTSVCWCPSDFTKIATCSDDNTLKVWRLNRDLEEKSGGDKLSIVGWASQKKREVKADLVAVPSSQSTPAKAPRAKSSPCTSSPSSAACAPSCAGDLPLPSNTPTFSLKTPPAKARSPISRRGSVSSVSPKPPSSFKMSIRNWMTRTASLSPPVTPPASETKITSPRKALIPVSLKSSQAVACSESRNRVKRRLDSSCLENVKQKCVKSCSCVTELDDPVEKLHLDLCCLAGAQEDLGKDSLGPTKPSKIEGASMSVSEPPSPASPYASEGCGTLPLPLGSCGEGSEVVGKENSSPENKNWLLAMAAKRKAENSSPRSPSSQTPNPRRQSGKTSPGPVTITPSSMRKICTYFHRKSQDDFCSPEQSTEL

Target ORF information:

RefSeq Version	XM_003583269.4
Organism	Bos taurus(cattle)
Definition	Bos taurus denticleless E3 ubiquitin protein ligase homolog (DTL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003583269.4

ORF Insert Sequence:

ATGCTCTTCA ATTCGGTGCT CCGCCAGCCC CAGTTTGGCG TCCCGAGAAA TGGATGGTCT 
TCACAGTACC CACTACAGTC CCTGCTCACT GGCTATCAGT GCAACTATAA TGACGAGCAT 
ACTTCTTATG GAGAAACAGG AACCCCAGTT CCTCCTTTTG GATGCACCTT CTCTTCTGCT 
CCCAGTATGG AGCATATACT AGCAGTTGCC AATGAAGAAG GCTTTGTTAG GTTATATAAC 
ACAGAATCAC ACACCAGTAG AAAGAAGTGC ATCAAAGAAT GGATGGCTCA CTGGAATGCT 
GTTTTTGACC TGGCCTGGGT CCCTGGTGAA TTTAAGCTTG TTACAGCAGC AGGTGATCAG 
ACAGCCAAAT TTTGGGATGT AAATGCTGGT GAGCTGATTG GAACTTGCAA AGGTCATCAG 
TGTAGCCTCA AGTCAGTTGC CTTTTCTAAG TTTGAGAAAG CTGTCTTCTG TACAGGGGGT 
AGAGATGGCA ACATTATGGT CTGGGATACC AGGTGCAACA AAAAAGATGG ATTTTACAGG 
CAAGTGAACC AAATCAGTGG AGCTCATAAT ACCTTAGACA AGCAAACCCC TTCAAAACCT 
AAGAAGAAAC AGAATTCCAA AGGGCTTGCT CCTTCTGTGG ATTTCCAGCA GAGTGTTACT 
GTGGTCCTCT TTCAGGATGA GAACACATTA GTCTCAGCAG GAGCTGTGGA TGGCATAATC 
AAAATATGGG ATTTACGTAA GAATTATACT GCTTATCGAC AGGAACCCAT AGCATCCAAG 
TCTTTCCTGT ACCCAGGTAG CAGCACTCGA AAACTAGGAT ACTCAAGTCT GATTTTGGAT 
TCTACTGGCT CCACTTTATT TGCTAACTGC ACAGATGACA ACATCTACAT GTTCAATATG 
ACTGGATTAA AGACTTCTCC AGTGGCTGTC TTCAGTGGAC ATCAGAACTC TACCTTTTAT 
GTTAAATCCA GTATTAGTCC AGATGACCAG TTTTTAGTCA GTGGCTCAAG TGATGAAGCT 
GCCTACATCT GGAAGGTCTC CATGCCCTGG CATCCTCCCA CTGTGCTCCT GGGTCATTCT 
CAAGAGGTCA CATCTGTGTG CTGGTGTCCA TCAGACTTCA CAAAGATTGC TACTTGCTCT 
GATGACAATA CACTGAAAGT CTGGCGCTTG AACAGAGATT TAGAGGAGAA ATCAGGAGGA 
GATAAACTCT CCATAGTGGG ATGGGCCTCT CAGAAGAAGA GAGAGGTGAA AGCCGACCTT 
GTGGCAGTGC CCAGCAGCCA GAGCACTCCG GCCAAAGCAC CCAGAGCAAA GAGCAGTCCG 
TGCACCTCCT CCCCGTCGTC GGCAGCCTGT GCTCCGAGCT GCGCCGGGGA CCTGCCCCTC 
CCTTCAAATA CACCTACCTT CTCTCTTAAG ACCCCTCCTG CCAAGGCCCG GTCTCCCATC 
AGCAGAAGAG GCTCTGTGTC CTCCGTCTCT CCCAAGCCAC CCTCATCTTT CAAGATGTCC 
ATTAGAAACT GGATGACCCG AACGGCTTCC TTATCACCAC CCGTCACTCC ACCTGCTTCC 
GAAACCAAGA TCACGTCTCC CAGAAAAGCC CTCATACCTG TGAGCCTGAA ATCATCCCAG 
GCAGTGGCTT GCTCTGAGTC TAGAAATAGA GTGAAGAGGA GGCTAGACTC CAGTTGTCTG 
GAGAATGTGA AACAAAAGTG TGTAAAGAGT TGCAGCTGTG TGACTGAGCT TGATGACCCC 
GTTGAAAAGC TTCATTTGGA TCTGTGCTGC CTTGCTGGTG CCCAGGAAGA CCTTGGGAAG 
GACTCGCTAG GTCCTACCAA ACCAAGCAAG ATCGAAGGAG CTAGTATGAG TGTCTCTGAG 
CCTCCTTCTC CTGCCAGTCC TTATGCTTCG GAAGGCTGTG GAACGCTACC TCTTCCTTTG 
GGATCTTGTG GAGAAGGCTC TGAAGTGGTA GGCAAAGAGA ATAGCTCCCC AGAGAATAAA 
AACTGGTTGT TGGCCATGGC AGCCAAACGG AAGGCGGAGA ATTCATCTCC ACGAAGTCCA 
TCGTCCCAGA CCCCCAATCC CAGGAGACAG AGCGGAAAGA CTTCTCCAGG CCCGGTCACC 
ATTACTCCCA GCTCCATGCG GAAAATCTGT ACATACTTCC ATAGAAAGTC CCAAGATGAC 
TTCTGTAGTC CAGAACAGTC AACAGAATTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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DTL cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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