ACSL1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACSL1
Entrez Gene ID	537161
Full Name	acyl-CoA synthetase long chain family member 1
Synonyms	FACL2
General protein information	Preferred Names acyl-CoA synthetase long chain family member 1 Names long-chain-fatty-acid--CoA ligase 1 fatty-acid-Coenzyme A ligase long chain 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005225993.4	XP_005226050.1	long-chain-fatty-acid--CoA ligase 1 isoform X2
XM_005225994.3	XP_005226051.1	long-chain-fatty-acid--CoA ligase 1 isoform X2
XM_005225994.4	XP_005226051.1	long-chain-fatty-acid--CoA ligase 1 isoform X2
NM_001076085.1	NP_001069553.1	long-chain-fatty-acid--CoA ligase 1
XM_010820261.2	XP_010818563.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_010820261.3	XP_010818563.1	long-chain-fatty-acid--CoA ligase 1 isoform X1
XM_005225993.3	XP_005226050.1	long-chain-fatty-acid--CoA ligase 1 isoform X2
XM_024986300.1	XP_024842068.1	long-chain-fatty-acid--CoA ligase 1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACSL1	NP_001273639.1
Mus musculus (house mouse)	Acsl1	NP_032007.2
Macaca mulatta (Rhesus monkey)	ACSL1	XP_001083742.2
Gallus gallus (chicken)	ACSL1	NP_001012596.1
Arabidopsis thaliana (thale cress)	LACS7	NP_198112.2
Xenopus tropicalis (tropical clawed frog)	acsl1	NP_001006830.1
Canis lupus familiaris (dog)	ACSL1	XP_005630044.1
Pan troglodytes (chimpanzee)	ACSL1	XP_003310629.1
Bos taurus (cattle)	ACSL1	NP_001069553.1
Rattus norvegicus (Norway rat)	Acsl1	NP_036952.1

Gene Ontology
Bibliography

Related articles in PubMed

Association of an ACSL1 gene variant with polyunsaturated fatty acids in bovine skeletal muscle.
Widmann P, Nuernberg K, Kuehn C, Weikard R
BMC genetics1296(2011 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

ACSL1, AGPAT6, FABP3, LPIN1, and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation.
Bionaz M, Loor JJ
The Journal of nutrition138(6)1019-24(2008 Jun)

High-resolution comparative mapping between human chromosomes 4 and 8 and bovine chromosome 27 provides genes and segments serving as positional candidates for udder health in cattle.
Goldammer T, Kata SR, Brunner RM, Kühn C, Weikard R, Womack JE, Schwerin M
Genomics84(4)696-706(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACSL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACSL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40254	NM_001076085.1 Latest version!	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa33927	XM_005225993.4 Latest version!	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa33927	XM_024986300.1 Latest version!	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa33927	XM_005225994.4 Latest version!	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa53547	XM_010820261.3 Latest version!	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBa33927	XM_005225994.3	Bos taurus acyl-CoA synthetase long-chain family member 1 (ACSL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa53547	XM_010820261.2	Bos taurus acyl-CoA synthetase long-chain family member 1 (ACSL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa33927	XM_005225993.3	Bos taurus acyl-CoA synthetase long-chain family member 1 (ACSL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACSL1 ( NM_001076085.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069553.1 Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), mRNA.

Price & Availability↑

CloneID	OBa40254
Clone ID Related Accession (Same CDS sequence)	NM_001076085.1
Accession Version	NM_001076085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC119914.1. On or before Oct 4, 2006 this sequence version replaced XM_876197.1, XM_617317.2. ##Evidence-Data-START## Transcript exon combination :: BC119914.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCCATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069553.1
CDS	75..2174
Translation	MMQAHELFQYFRLPELLDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPRALKPPCDLAMQSVEVPGSDGARRSTLLDSDEPLVYFYDDVRTLYEGFQRGLHVSNNGPCLGSRKPDQPYEWLSYKQVEDMAECVGSALLHKGFKAAPDQFIGIFAQNRPEWVIIEQGCFTYSMVIVPLYDTLGTEAITYIINKAELSLVFVDKPEKANLLLEGVENKLIPCLKTIVLMDSYGSDLLERGKKCGVEIISMKAMEDLGRANRQKPKPPVPEDLAVICFTSGTTGNPKGAMITHRNVVSDCSAFVKMTENTFIPTSDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKALQPTIFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTVAGDWTAGHVGPPMPCSIIKLVDVEEMNYLAAKGEGEVCVKGSNVFQGYLKDPVKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYQRSEPIAQVFVHGESLQAFLIAIVVPDVETLGTWAQKRGIVGSFEELCRNKDVKKGILEDMVRLGKESGLKPFEQVKGIYLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDELYSTIKV

Target ORF information:

RefSeq Version	NM_001076085.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076085.1

ORF Insert Sequence:

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCCATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL1 ( XM_005225993.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226050.1 Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa33927
Clone ID Related Accession (Same CDS sequence)	XM_005225993.4 , XM_005225994.3 , XM_005225994.4 , XM_005225993.3 , XM_024986300.1
Accession Version	XM_005225993.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225993.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226050.1
CDS	193..2292
Translation	MMQAHELFQYFRLPELLDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPRALKPPCDLAMQSVEVPGSDGARRSTLLDSDEPLVYFYDDVRTLYEGFQRGLHVSNNGPCLGSRKPDQPYEWLSYKQVEDMAECVGSALLHKGFKAAPDQFIGIFAQNRPEWVIIEQGCFTYSMVIVPLYDTLGTEAITYIINKAELSLVFVDKPEKANLLLEGVENKLIPCLKTIVLMDSYGSDLLERGKKCGVEIISMKAMEDLGRANRQKPKPPVPEDLAVICFTSGTTGNPKGAMITHRNVVSDCSAFVKMTENTFIPTSDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKALQPTIFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTVAGDWTAGHVGPPMPCSIIKLVDVEEMNYLAAKGEGEVCVKGSNVFQGYLKDPVKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYQRSEPIAQVFVHGESLQAFLIAIVVPDVETLGTWAQKRGIVGSFEELCRNKDVKKGILEDMVRLGKESGLKPFEQVKGIYLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDELYSTIKV

Target ORF information:

RefSeq Version	XM_005225993.4
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225993.4

ORF Insert Sequence:

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL1 ( XM_005225994.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226051.1 Bos taurus acyl-CoA synthetase long-chain family member 1 (ACSL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa33927
Clone ID Related Accession (Same CDS sequence)	XM_005225993.4 , XM_005225994.3 , XM_005225994.4 , XM_005225993.3 , XM_024986300.1
Accession Version	XM_005225994.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225994.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226051.1
CDS	232..2331
Translation	MMQAHELFQYFRLPELLDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPRALKPPCDLAMQSVEVPGSDGARRSTLLDSDEPLVYFYDDVRTLYEGFQRGLHVSNNGPCLGSRKPDQPYEWLSYKQVEDMAECVGSALLHKGFKAAPDQFIGIFAQNRPEWVIIEQGCFTYSMVIVPLYDTLGTEAITYIINKAELSLVFVDKPEKANLLLEGVENKLIPCLKTIVLMDSYGSDLLERGKKCGVEIISMKAMEDLGRANRQKPKPPVPEDLAVICFTSGTTGNPKGAMITHRNVVSDCSAFVKMTENTFIPTSDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKALQPTIFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTVAGDWTAGHVGPPMPCSIIKLVDVEEMNYLAAKGEGEVCVKGSNVFQGYLKDPVKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYQRSEPIAQVFVHGESLQAFLIAIVVPDVETLGTWAQKRGIVGSFEELCRNKDVKKGILEDMVRLGKESGLKPFEQVKGIYLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDELYSTIKV

Target ORF information:

RefSeq Version	XM_005225994.3
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase long-chain family member 1 (ACSL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225994.3

ORF Insert Sequence:

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL1 ( XM_005225994.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226051.1 Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa33927
Clone ID Related Accession (Same CDS sequence)	XM_005225993.4 , XM_005225994.3 , XM_005225994.4 , XM_005225993.3 , XM_024986300.1
Accession Version	XM_005225994.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225994.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226051.1
CDS	232..2331
Translation	MMQAHELFQYFRLPELLDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPRALKPPCDLAMQSVEVPGSDGARRSTLLDSDEPLVYFYDDVRTLYEGFQRGLHVSNNGPCLGSRKPDQPYEWLSYKQVEDMAECVGSALLHKGFKAAPDQFIGIFAQNRPEWVIIEQGCFTYSMVIVPLYDTLGTEAITYIINKAELSLVFVDKPEKANLLLEGVENKLIPCLKTIVLMDSYGSDLLERGKKCGVEIISMKAMEDLGRANRQKPKPPVPEDLAVICFTSGTTGNPKGAMITHRNVVSDCSAFVKMTENTFIPTSDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKALQPTIFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTVAGDWTAGHVGPPMPCSIIKLVDVEEMNYLAAKGEGEVCVKGSNVFQGYLKDPVKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYQRSEPIAQVFVHGESLQAFLIAIVVPDVETLGTWAQKRGIVGSFEELCRNKDVKKGILEDMVRLGKESGLKPFEQVKGIYLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDELYSTIKV

Target ORF information:

RefSeq Version	XM_005225994.4
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225994.4

ORF Insert Sequence:

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL1 ( XM_005225993.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226050.1 Bos taurus acyl-CoA synthetase long-chain family member 1 (ACSL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa33927
Clone ID Related Accession (Same CDS sequence)	XM_005225993.4 , XM_005225994.3 , XM_005225994.4 , XM_005225993.3 , XM_024986300.1
Accession Version	XM_005225993.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226050.1
CDS	193..2292
Translation	MMQAHELFQYFRLPELLDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPRALKPPCDLAMQSVEVPGSDGARRSTLLDSDEPLVYFYDDVRTLYEGFQRGLHVSNNGPCLGSRKPDQPYEWLSYKQVEDMAECVGSALLHKGFKAAPDQFIGIFAQNRPEWVIIEQGCFTYSMVIVPLYDTLGTEAITYIINKAELSLVFVDKPEKANLLLEGVENKLIPCLKTIVLMDSYGSDLLERGKKCGVEIISMKAMEDLGRANRQKPKPPVPEDLAVICFTSGTTGNPKGAMITHRNVVSDCSAFVKMTENTFIPTSDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKALQPTIFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTVAGDWTAGHVGPPMPCSIIKLVDVEEMNYLAAKGEGEVCVKGSNVFQGYLKDPVKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYQRSEPIAQVFVHGESLQAFLIAIVVPDVETLGTWAQKRGIVGSFEELCRNKDVKKGILEDMVRLGKESGLKPFEQVKGIYLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDELYSTIKV

Target ORF information:

RefSeq Version	XM_005225993.3
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase long-chain family member 1 (ACSL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225993.3

ORF Insert Sequence:

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL1 ( XM_024986300.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842068.1 Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X4, mRNA.

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CloneID	OBa33927
Clone ID Related Accession (Same CDS sequence)	XM_005225993.4 , XM_005225994.3 , XM_005225994.4 , XM_005225993.3 , XM_024986300.1
Accession Version	XM_024986300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842068.1
CDS	745..2844
Translation	MMQAHELFQYFRLPELLDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPRALKPPCDLAMQSVEVPGSDGARRSTLLDSDEPLVYFYDDVRTLYEGFQRGLHVSNNGPCLGSRKPDQPYEWLSYKQVEDMAECVGSALLHKGFKAAPDQFIGIFAQNRPEWVIIEQGCFTYSMVIVPLYDTLGTEAITYIINKAELSLVFVDKPEKANLLLEGVENKLIPCLKTIVLMDSYGSDLLERGKKCGVEIISMKAMEDLGRANRQKPKPPVPEDLAVICFTSGTTGNPKGAMITHRNVVSDCSAFVKMTENTFIPTSDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKALQPTIFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTVAGDWTAGHVGPPMPCSIIKLVDVEEMNYLAAKGEGEVCVKGSNVFQGYLKDPVKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYQRSEPIAQVFVHGESLQAFLIAIVVPDVETLGTWAQKRGIVGSFEELCRNKDVKKGILEDMVRLGKESGLKPFEQVKGIYLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDELYSTIKV

Target ORF information:

RefSeq Version	XM_024986300.1
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986300.1

ORF Insert Sequence:

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA CACGTTCATT CCTACCTCAG ATGACACTTT GATCTCTTTC 
TTGCCTCTGG CCCATATGTT TGAGAGAGTT GTAGAGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL1 ( XM_010820261.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818563.1 Bos taurus acyl-CoA synthetase long-chain family member 1 (ACSL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa53547
Clone ID Related Accession (Same CDS sequence)	XM_010820261.2 , XM_010820261.3
Accession Version	XM_010820261.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA AGCACTTGTG TTGAATACCA GTGACATACA CATTTCGTTT 
TTACCGCTTG CACACATGTA TGAGCAGCTC ATGCTGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818563.1
CDS	30..2129
Translation	MMQAHELFQYFRLPELLDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPRALKPPCDLAMQSVEVPGSDGARRSTLLDSDEPLVYFYDDVRTLYEGFQRGLHVSNNGPCLGSRKPDQPYEWLSYKQVEDMAECVGSALLHKGFKAAPDQFIGIFAQNRPEWVIIEQGCFTYSMVIVPLYDTLGTEAITYIINKAELSLVFVDKPEKANLLLEGVENKLIPCLKTIVLMDSYGSDLLERGKKCGVEIISMKAMEDLGRANRQKPKPPVPEDLAVICFTSGTTGNPKGAMITHRNVVSDCSAFVKMTEKALVLNTSDIHISFLPLAHMYEQLMLCVMLCHGAKIGFFQGDIRLLMDDLKALQPTIFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTVAGDWTAGHVGPPMPCSIIKLVDVEEMNYLAAKGEGEVCVKGSNVFQGYLKDPVKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYQRSEPIAQVFVHGESLQAFLIAIVVPDVETLGTWAQKRGIVGSFEELCRNKDVKKGILEDMVRLGKESGLKPFEQVKGIYLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDELYSTIKV

Target ORF information:

RefSeq Version	XM_010820261.2
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase long-chain family member 1 (ACSL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820261.2

ORF Insert Sequence:

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA AGCACTTGTG TTGAATACCA GTGACATACA CATTTCGTTT 
TTACCGCTTG CACACATGTA TGAGCAGCTC ATGCTGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSL1 ( XM_010820261.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818563.1 Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X1, mRNA.

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CloneID	OBa53547
Clone ID Related Accession (Same CDS sequence)	XM_010820261.2 , XM_010820261.3
Accession Version	XM_010820261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	long-chain-fatty-acid--CoA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA AGCACTTGTG TTGAATACCA GTGACATACA CATTTCGTTT 
TTACCGCTTG CACACATGTA TGAGCAGCTC ATGCTGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818563.1
CDS	30..2129
Translation	MMQAHELFQYFRLPELLDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPRALKPPCDLAMQSVEVPGSDGARRSTLLDSDEPLVYFYDDVRTLYEGFQRGLHVSNNGPCLGSRKPDQPYEWLSYKQVEDMAECVGSALLHKGFKAAPDQFIGIFAQNRPEWVIIEQGCFTYSMVIVPLYDTLGTEAITYIINKAELSLVFVDKPEKANLLLEGVENKLIPCLKTIVLMDSYGSDLLERGKKCGVEIISMKAMEDLGRANRQKPKPPVPEDLAVICFTSGTTGNPKGAMITHRNVVSDCSAFVKMTEKALVLNTSDIHISFLPLAHMYEQLMLCVMLCHGAKIGFFQGDIRLLMDDLKALQPTIFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTVAGDWTAGHVGPPMPCSIIKLVDVEEMNYLAAKGEGEVCVKGSNVFQGYLKDPVKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYQRSEPIAQVFVHGESLQAFLIAIVVPDVETLGTWAQKRGIVGSFEELCRNKDVKKGILEDMVRLGKESGLKPFEQVKGIYLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDELYSTIKV

Target ORF information:

RefSeq Version	XM_010820261.3
Organism	Bos taurus(cattle)
Definition	Bos taurus acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820261.3

ORF Insert Sequence:

ATGATGCAAG CCCACGAGCT GTTCCAGTAC TTTCGGCTGC CAGAGCTGCT TGACTTCCGG 
CAGTACGTGC GCACCCTTCC AACCAACACG CTCATGGGCT TCGGCGCATT CGCGGCGCTC 
ACCACCTTCT GGTACGCCAC GCGGCCACGG GCCCTGAAGC CTCCATGCGA CCTGGCCATG 
CAGTCAGTGG AGGTGCCGGG CAGTGATGGT GCCCGGAGAT CCACGCTCCT GGACAGTGAC 
GAGCCCCTGG TGTATTTCTA CGATGACGTC AGAACGTTAT ACGAAGGTTT CCAGAGGGGC 
TTACACGTGT CCAATAATGG CCCTTGTTTA GGCTCTCGGA AACCAGACCA ACCCTATGAA 
TGGCTTTCAT ACAAACAGGT TGAAGATATG GCAGAGTGTG TGGGCTCAGC ACTGCTCCAC 
AAGGGCTTCA AGGCAGCTCC AGACCAGTTT ATTGGCATCT TCGCTCAGAA CAGACCTGAG 
TGGGTGATCA TTGAACAGGG ATGCTTTACT TACTCGATGG TGATCGTTCC CCTCTACGAC 
ACCCTCGGAA CTGAAGCCAT CACCTACATC ATCAACAAAG CCGAACTCTC TTTGGTTTTT 
GTGGACAAGC CAGAGAAGGC CAACCTCCTA TTAGAAGGTG TAGAAAATAA ATTAATCCCA 
TGCCTTAAAA CTATTGTTCT CATGGACTCC TACGGCAGTG ATCTGCTGGA ACGAGGCAAG 
AAGTGTGGGG TGGAGATCAT CAGCATGAAG GCCATGGAGG ACTTGGGAAG AGCCAACAGA 
CAGAAGCCCA AGCCTCCAGT ACCTGAAGAT CTTGCAGTAA TTTGCTTCAC CAGTGGAACT 
ACAGGCAACC CCAAAGGAGC AATGATCACG CATCGAAATG TAGTGAGCGA TTGTTCAGCA 
TTTGTGAAAA TGACAGAGAA AGCACTTGTG TTGAATACCA GTGACATACA CATTTCGTTT 
TTACCGCTTG CACACATGTA TGAGCAGCTC ATGCTGTGTG TGATGCTCTG TCATGGAGCT 
AAAATAGGAT TTTTCCAAGG AGATATCAGG CTGCTTATGG ATGATCTCAA GGCCCTTCAA 
CCCACCATCT TCCCCGTGGT CCCCAGACTG CTGAACCGGA TGTTTGACCG AATTTTCGGA 
CAAGCAAACA CTACGCTGAA GCGATGGCTG TTGGACTTTG CCTCCAAGAG GAAAGAGGCT 
GAACTTCGCA GTGGCATCAT TAGAAACAAC AGCCTCTGGG ATAAACTGAT CTTCCACAAA 
ATACAGTCGA GCCTGGGAGG AAAAGTGAGG CTGATGATCA CAGGAGCAGC GCCTGTGTCT 
GCCACCGTGC TCACCTTCCT GAGGGCTGCG CTGGGCTGCC AGTTCTACGA AGGCTACGGA 
CAGACCGAGT GCACCGCTGG CTGCTGCCTG ACTGTTGCTG GAGACTGGAC CGCAGGCCAT 
GTTGGTCCCC CAATGCCTTG CAGCATTATC AAGCTTGTTG ATGTGGAAGA AATGAATTAC 
CTGGCTGCCA AGGGCGAGGG CGAGGTATGT GTGAAAGGGT CAAATGTTTT TCAAGGCTAC 
TTGAAAGACC CAGTGAAAAC AGCAGAAGCT CTGGATAAAG ACGGCTGGTT GCACACAGGG 
GACATTGGCA AGTGGTTACC CAATGGCACC TTGAAGATCA TCGACAGGAA GAAGCACATA 
TTTAAACTGG CACAGGGGGA GTACATAGCT CCCGAGAAGA TCGAAAACAT CTACCAGCGG 
AGTGAACCTA TCGCTCAGGT GTTTGTCCAC GGAGAGAGCT TGCAGGCATT TCTCATAGCA 
ATCGTGGTGC CGGACGTTGA GACACTAGGT ACCTGGGCCC AGAAGAGAGG CATTGTGGGC 
TCCTTTGAAG AACTGTGCAG AAACAAGGAT GTCAAAAAAG GTATTCTAGA AGACATGGTG 
AGGCTCGGGA AGGAGTCTGG CCTAAAACCA TTTGAACAGG TCAAAGGCAT CTATCTCCAC 
CCTGAACTCT TCTCTATCGA CAATGGCCTT CTGACCCCAA CCATGAAGGC TAAAAGGCCA 
GAGCTTCGGA ATTATTTCAG GTCACAGATC GATGAGCTTT ACTCCACCAT CAAAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of an ACSL1 gene variant with polyunsaturated fatty acids in bovine skeletal muscle.
BMC genetics1296(2011 Nov)
Widmann P,Nuernberg K,Kuehn C,Weikard R

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

ACSL1, AGPAT6, FABP3, LPIN1, and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation.
The Journal of nutrition138(6)1019-24(2008 Jun)
Bionaz M,Loor JJ

High-resolution comparative mapping between human chromosomes 4 and 8 and bovine chromosome 27 provides genes and segments serving as positional candidates for udder health in cattle.
Genomics84(4)696-706(2004 Oct)
Goldammer T,Kata SR,Brunner RM,Kühn C,Weikard R,Womack JE,Schwerin M

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ACSL1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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