BUB1B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1B
Entrez Gene ID	537027
Full Name	BUB1 mitotic checkpoint serine/threonine kinase B
General protein information	Preferred Names BUB1 mitotic checkpoint serine/threonine kinase B Names mitotic checkpoint serine/threonine-protein kinase BUB1 beta BUB1 budding uninhibited by benzimidazoles 1 homolog beta budding uninhibited by benzimidazoles 1 homolog beta
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001145173.1	NP_001138645.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
XM_024997695.1	XP_024853463.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	BUB1B	NP_001202.4
Pan troglodytes (chimpanzee)	BUB1B	XP_510299.3
Canis lupus familiaris (dog)	BUB1B	XP_850443.2
Macaca mulatta (Rhesus monkey)	BUB1B	XP_002804771.1
Gallus gallus (chicken)	BUB1B	NP_989734.1
Bos taurus (cattle)	BUB1B	NP_001138645.1
Arabidopsis thaliana (thale cress)	BUBR1	NP_565767.1
Oryza sativa (rice)	Os02g0193600	NP_001046171.1
Mus musculus (house mouse)	Bub1b	NP_033903.2
Rattus norvegicus (Norway rat)	Bub1b	XP_342495.4
Danio rerio (zebrafish)	bub1bb	XP_695384.3
Xenopus tropicalis (tropical clawed frog)	bub1b	NP_001096484.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BUB1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa06476	NM_001145173.1 Latest version!	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa219650	XM_024997695.1 Latest version!	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1B ( NM_001145173.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001138645.1 Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Price & Availability↑

CloneID	OBa06476
Clone ID Related Accession (Same CDS sequence)	NM_001145173.1
Accession Version	NM_001145173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527868800000
Organism	Bos taurus(cattle)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000010.1. On Feb 13, 2009 this sequence version replaced XM_617181.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571262.21607.1, SRR5571257.48774.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145426, SAMN03145433 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGA TGCAGAAGGA AAGGGGTGCT CCGAGTGACA TCATGTCCTT GGAGGGAGAT 
GAATGGGAGC TGAGTAAAGA AAATGTCCAA CCTTTAAGGC AGGGGCGGAT CATGTCCACA 
CTTCAGGGAG CTCTGGCACA GCAAGAATCT GCCTGTAACA CTACTCTTCA ACAACAAAAA 
CGAGCATTTG AATCTGAAAT TCGATTTTAT GCTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGATATATTA ACTGGACAGA GCAAAACTAT CCTCAGGGCG GGAAGGAAAG CAATATGTCA 
ACATTACTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AACGATATTA TAATGATCCT 
CGATTTCTTA GTCTCTGGCT TAAGCTGGGG CATTTATGCA ATGAACCTTT GGATATGTAC 
AGTTATTTAC ATAACCAAGG AATTGGCATT TCACTTGCTC AGTTTTATAT CTCATGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGATC TGATATTTCA AGAAGGTATT 
GAACAGAAGG CTGAACCACT GGAAAGACTG CAGTCCCAGC ACAGACAATT CCAAGCTCGA 
GTGTCTCGGC AAACTCTGTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TGTGGGATCT 
TCTGTACCAC AGCGAAGCAC ACTGGCTGAA CTAAAAAGCA AAGGGAAAAA GACAGCAAGA 
GCTCCAATCA GCCGTGTAGG GGGTGCTCTC AAGGCTCCAA GCCAGAACAG AGGTCTCCAA 
AATCCAGTTC CTCAACAGAT GCAAAGTAAT ACTAGAATTA TTGTTTTTGA TGAAAATACT 
GATGAGGCTT CTAGAGCAGA GCCATCTAAG CCTACAGTCC AGCCATGGAT AGCACCTCCT 
GTGTCCAGGG GCAAAGAGAA TGAGCTGCAA GCCGGCCCTT GGAACACAGG CAGGCCTTTG 
GAATACAGGC CTCGAGGCAA TACAGCGTCT ATGACAGCTG TACCCTCTAT GCTTCCAAGT 
TTTACTCCAT ATGTGGAAGA GACTGCACAA CAGCCAGTTA TGACACCATG TAAAATTGAA 
CCCAGTATAA ACCACATTCT AAGCACAAGA AAGCCTGGAA AGGAAGAAGG AGATCTCCTG 
CAGAGAGTTC AAAGTCTTCA GCAAGAGTCT GAGAAGAAGG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT GGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAGGTTTTC 
CGGAAGAAAT TAAAAGAGCG AAGAGAAGCT GAGCTACTGA CCAGTGCAGA GAAAAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG 
CAAGAAAGAG CGAATGAACA GCAAGAAGAA AAGATGCCTA CAAAGGAGAC AGCTGAACTG 
CCAATTGCTT CAGAGTCTCA GGAAATATCA GGAATGGCTC TGTCCAATTT TGTTTGCCCA 
GTAAACTCTT GTGCCAGGGA AACATCACTT GCAGAAAACG TTTGGCAAGA ACAACCTCAT 
TCTAAAAGTT CCAGTGTACC TTTCTCCATT TTTGATGAAT CTCTTCTTTC AGAAAAAAAG 
CCTGTCAGTC CTTCTGCAGC TCCCTCACGT GTTTTAGCCC AACGAAGACC TCTTGCAATT 
CTCAAAACTT CAGAAAGCAT CACCTCAAAT GAGGATGTAT CTCCAGATGT TTGTGATGAA 
TTTACAGGAA TTGAGCCCTT GAGTGAGGAT GCTATTATCA CAGGTTTCAG GAATGTAACT 
ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTCGCTAGAG CAGCTCGTTT TGCATCCACT 
CCTTTTCATG AAATAATATC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTGCAG 
GAAGAGGATC TAGATATGAA GACCTCTGAG GACCAGCAAA CTGCTTGTGG CACTATCTAC 
AATCCACCTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGCCG TGAAGCCACA 
CATTCCTCTG GGTTCTCCGG ATCTTCTGCC TCAGTTACAA GCACCTCCTC CATCAAAAGT 
CTTCAAATTC CTGAGAAACT GGAGCTCACT AGTGATACTG CAGACAGCCC TACTCAGTCA 
CCCTGGTGTC CACAGTATCG CAGACAACTG GTAAAATCCC TACCAGAGCT GAGTGTCTCT 
ACAGAGTTTT TTGTAGAAGA TAGACCAATG CCTAGTTTGG AAATAGAGAA GGAAATTGAA 
TTAGATAATG AGGAATATTG CATTAGACAA GAATACCTGG TATGTGAAGA CTACAAATTA 
TTCTGGGTGA CACCAAGAAA CACTGCCGAG TTAACCATAA TAAAGGTATC TTCTCATCCT 
GTCCCATGGG ACTTCTATAT CAACTTCAAG TTAAAGGAAC GTTTAAATGA GGAGTGTGAT 
AATTTGTGTA GCTGTTATCA ATACCAAGAT GGCTGTATCG TTTGGCATCA ATATATAAAC 
TGCTTCACCC TTCAGGATCT TCTTCAACAC AGTGAATTTA TTACCCATGA AATGACGGTA 
TTGATTATTT ATAACCTTTT GACAATGGTG GAGAAACTAC ACAAAGCAGA AATAGTCCAT 
GGTGACTTGA GTCCAAGGAG CCTGATTCTT AGAAACAGAA TCCACGATCC CTATGATTTT 
AATAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGCA 
CAGCTAGACG TTTTTAACCT CAGTGGCTTT CGGACTGTAC AAACCCTGGA AGGACAAAAG 
ATCCTGGCTA ACTGTTCTTC TCCATACCAG GTAGATCTGT TTGGTATAGC AGATTTAGCA 
CATTTATTAT TGTTCAAGGA GCACCTACAG GTCTTCTGGG ATGGTTCCCT CTGGAAACTT 
AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TTTGCGGATT 
CTGAATGCCA GTGATGAGTC CACAGTGTCT GTTCTGGGGG AACTTGCAGC AGAAATGAAT 
GGGGTGTTTG ACACCACATT CCAAAGTCAC CTGAACAAAG CTTTTTGGAA AGTGGGGAAG 
TTAATCAGTC CTGGGGCTTT CCTCTTCCAG CAAGATAGGC AGTCAAGCCT CTCACAGATT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138645.1
CDS	201..3389
Translation	MAAMQKERGAPSDIMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQQESACNTTLQQQKRAFESEIRFYAGNDPLDVWDRYINWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYNDPRFLSLWLKLGHLCNEPLDMYSYLHNQGIGISLAQFYISWAEEYEARENFKKADLIFQEGIEQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVVGSSVPQRSTLAELKSKGKKTARAPISRVGGALKAPSQNRGLQNPVPQQMQSNTRIIVFDENTDEASRAEPSKPTVQPWIAPPVSRGKENELQAGPWNTGRPLEYRPRGNTASMTAVPSMLPSFTPYVEETAQQPVMTPCKIEPSINHILSTRKPGKEEGDLLQRVQSLQQESEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKERREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERANEQQEEKMPTKETAELPIASESQEISGMALSNFVCPVNSCARETSLAENVWQEQPHSKSSSVPFSIFDESLLSEKKPVSPSAAPSRVLAQRRPLAILKTSESITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFASTPFHEIISLKDLPSDPERLLQEEDLDMKTSEDQQTACGTIYNPPLSIKKLSPIIEDSREATHSSGFSGSSASVTSTSSIKSLQIPEKLELTSDTADSPTQSPWCPQYRRQLVKSLPELSVSTEFFVEDRPMPSLEIEKEIELDNEEYCIRQEYLVCEDYKLFWVTPRNTAELTIIKVSSHPVPWDFYINFKLKERLNEECDNLCSCYQYQDGCIVWHQYINCFTLQDLLQHSEFITHEMTVLIIYNLLTMVEKLHKAEIVHGDLSPRSLILRNRIHDPYDFNKNNQALKIVDFSYSVDLRAQLDVFNLSGFRTVQTLEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSLWKLSQNISELKDGELWNKFFLRILNASDESTVSVLGELAAEMNGVFDTTFQSHLNKAFWKVGKLISPGAFLFQQDRQSSLSQIPA

Target ORF information:

RefSeq Version	NM_001145173.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145173.1

ORF Insert Sequence:

ATGGCGGCGA TGCAGAAGGA AAGGGGTGCT CCGAGTGACA TCATGTCCTT GGAGGGAGAT 
GAATGGGAGC TGAGTAAAGA AAATGTCCAA CCTTTAAGGC AGGGGCGGAT CATGTCCACA 
CTTCAGGGAG CTCTGGCACA GCAAGAATCT GCCTGTAACA CTACTCTTCA ACAACAAAAA 
CGAGCATTTG AATCTGAAAT TCGATTTTAT GCTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGATATATTA ACTGGACAGA GCAAAACTAT CCTCAGGGCG GGAAGGAAAG CAATATGTCA 
ACATTACTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AACGATATTA TAATGATCCT 
CGATTTCTTA GTCTCTGGCT TAAGCTGGGG CATTTATGCA ATGAACCTTT GGATATGTAC 
AGTTATTTAC ATAACCAAGG AATTGGCATT TCACTTGCTC AGTTTTATAT CTCATGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGATC TGATATTTCA AGAAGGTATT 
GAACAGAAGG CTGAACCACT GGAAAGACTG CAGTCCCAGC ACAGACAATT CCAAGCTCGA 
GTGTCTCGGC AAACTCTGTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TGTGGGATCT 
TCTGTACCAC AGCGAAGCAC ACTGGCTGAA CTAAAAAGCA AAGGGAAAAA GACAGCAAGA 
GCTCCAATCA GCCGTGTAGG GGGTGCTCTC AAGGCTCCAA GCCAGAACAG AGGTCTCCAA 
AATCCAGTTC CTCAACAGAT GCAAAGTAAT ACTAGAATTA TTGTTTTTGA TGAAAATACT 
GATGAGGCTT CTAGAGCAGA GCCATCTAAG CCTACAGTCC AGCCATGGAT AGCACCTCCT 
GTGTCCAGGG GCAAAGAGAA TGAGCTGCAA GCCGGCCCTT GGAACACAGG CAGGCCTTTG 
GAATACAGGC CTCGAGGCAA TACAGCGTCT ATGACAGCTG TACCCTCTAT GCTTCCAAGT 
TTTACTCCAT ATGTGGAAGA GACTGCACAA CAGCCAGTTA TGACACCATG TAAAATTGAA 
CCCAGTATAA ACCACATTCT AAGCACAAGA AAGCCTGGAA AGGAAGAAGG AGATCTCCTG 
CAGAGAGTTC AAAGTCTTCA GCAAGAGTCT GAGAAGAAGG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT GGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAGGTTTTC 
CGGAAGAAAT TAAAAGAGCG AAGAGAAGCT GAGCTACTGA CCAGTGCAGA GAAAAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG 
CAAGAAAGAG CGAATGAACA GCAAGAAGAA AAGATGCCTA CAAAGGAGAC AGCTGAACTG 
CCAATTGCTT CAGAGTCTCA GGAAATATCA GGAATGGCTC TGTCCAATTT TGTTTGCCCA 
GTAAACTCTT GTGCCAGGGA AACATCACTT GCAGAAAACG TTTGGCAAGA ACAACCTCAT 
TCTAAAAGTT CCAGTGTACC TTTCTCCATT TTTGATGAAT CTCTTCTTTC AGAAAAAAAG 
CCTGTCAGTC CTTCTGCAGC TCCCTCACGT GTTTTAGCCC AACGAAGACC TCTTGCAATT 
CTCAAAACTT CAGAAAGCAT CACCTCAAAT GAGGATGTAT CTCCAGATGT TTGTGATGAA 
TTTACAGGAA TTGAGCCCTT GAGTGAGGAT GCTATTATCA CAGGTTTCAG GAATGTAACT 
ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTCGCTAGAG CAGCTCGTTT TGCATCCACT 
CCTTTTCATG AAATAATATC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTGCAG 
GAAGAGGATC TAGATATGAA GACCTCTGAG GACCAGCAAA CTGCTTGTGG CACTATCTAC 
AATCCACCTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGCCG TGAAGCCACA 
CATTCCTCTG GGTTCTCCGG ATCTTCTGCC TCAGTTACAA GCACCTCCTC CATCAAAAGT 
CTTCAAATTC CTGAGAAACT GGAGCTCACT AGTGATACTG CAGACAGCCC TACTCAGTCA 
CCCTGGTGTC CACAGTATCG CAGACAACTG GTAAAATCCC TACCAGAGCT GAGTGTCTCT 
ACAGAGTTTT TTGTAGAAGA TAGACCAATG CCTAGTTTGG AAATAGAGAA GGAAATTGAA 
TTAGATAATG AGGAATATTG CATTAGACAA GAATACCTGG TATGTGAAGA CTACAAATTA 
TTCTGGGTGA CACCAAGAAA CACTGCCGAG TTAACCATAA TAAAGGTATC TTCTCATCCT 
GTCCCATGGG ACTTCTATAT CAACTTCAAG TTAAAGGAAC GTTTAAATGA GGAGTGTGAT 
AATTTGTGTA GCTGTTATCA ATACCAAGAT GGCTGTATCG TTTGGCATCA ATATATAAAC 
TGCTTCACCC TTCAGGATCT TCTTCAACAC AGTGAATTTA TTACCCATGA AATGACGGTA 
TTGATTATTT ATAACCTTTT GACAATGGTG GAGAAACTAC ACAAAGCAGA AATAGTCCAT 
GGTGACTTGA GTCCAAGGAG CCTGATTCTT AGAAACAGAA TCCACGATCC CTATGATTTT 
AATAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGCA 
CAGCTAGACG TTTTTAACCT CAGTGGCTTT CGGACTGTAC AAACCCTGGA AGGACAAAAG 
ATCCTGGCTA ACTGTTCTTC TCCATACCAG GTAGATCTGT TTGGTATAGC AGATTTAGCA 
CATTTATTAT TGTTCAAGGA GCACCTACAG GTCTTCTGGG ATGGTTCCCT CTGGAAACTT 
AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TTTGCGGATT 
CTGAATGCCA GTGATGAGTC CACAGTGTCT GTTCTGGGGG AACTTGCAGC AGAAATGAAT 
GGGGTGTTTG ACACCACATT CCAAAGTCAC CTGAACAAAG CTTTTTGGAA AGTGGGGAAG 
TTAATCAGTC CTGGGGCTTT CCTCTTCCAG CAAGATAGGC AGTCAAGCCT CTCACAGATT 
CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1B ( XM_024997695.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853463.1 Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219650
Clone ID Related Accession (Same CDS sequence)	XM_024997695.1
Accession Version	XM_024997695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TGCAGAAGGA AAGGGGTGCT CCGAGTGACA TCATGTCCTT GGAGGGAGAT 
GAATGGGAGC TGAGTAAAGA AAATGTCCAA CCTTTAAGGC AGGGGCGGAT CATGTCCACA 
CTTCAGGGAG CTCTGGCACA GCAAGAATCT GCCTGTAACA CTACTCTTCA ACAACAAAAA 
CGAGCATTTG AATCTGAAAT TCGATTTTAT GCTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGATATATTA ACTGGACAGA GCAAAACTAT CCTCAGGGCG GGAAGGAAAG CAATATGTCA 
ACATTACTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AACGATATTA TAATGATCCT 
CGATTTCTTA GTCTCTGGCT TAAGCTGGGG CATTTATGCA ATGAACCTTT GGATATGTAC 
AGTTATTTAC ATAACCAAGG AATTGGCATT TCACTTGCTC AGTTTTATAT CTCATGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGATC TGATATTTCA AGAAGGTATT 
GAACAGAAGG CTGAACCACT GGAAAGACTG CAGTCCCAGC ACAGACAATT CCAAGCTCGA 
GTGTCTCGGC AAACTCTGTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TGTGGGATCT 
TCTGTACCAC AGCGAAGCAC ACTGGCTGAA CTAAAAAGCA AAGGGAAAAA GACAGCAAGA 
GCTCCAATCA GCCGTGTAGG GGGTGCTCTC AAGGCTCCAA GCCAGAACAG AGGTCTCCAA 
AATCCAGTTC CTCAACAGAT GCAAAGTAAT ACTAGAATTA TTGTTTTTGA TGAAAATACT 
GATGAGGCTT CTAGAGCAGA GCCATCTAAG CCTACAGTCC AGCCATGGAT AGCACCTCCT 
GTGTCCAGGG GCAAAGAGAA TGAGCTGCAA GCCGGCCCTT GGAACACAGG CAGGCCTTTG 
GAATACAGGC CTCGAGGCAA TACAGCGTCT ATGACAGCTG TACCCTCTAT GCTTCCAAGT 
TTTACTCCAT ATGTGGAAGA GACTGCACAA CAGCCAGTTA TGACACCATG TAAAATTGAA 
CCCAGTATAA ACCACATTCT AAGCACAAGA AAGCCTGGAA AGGAAGAAGG AGATCTCCTG 
CAGAGAGTTC AAAGTCTTCA GCAAGAGTCT GAGAAGAAGG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT GGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAGGTTTTC 
CGGAAGAAAT TAAAAGAGCG AAGAGAAGCT GAGCTACTGA CCAGTGCAGA GAAAAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG 
CAAGAAAGAG CGAATGAACA GCAAGAAGAA AAGATGCCTA CAAAGGAGAC AGCTGAACTG 
CCAATTGCTT CAGAGTCTCA GGAAATATCA GGAATGGCTC TGTCCAATTT TGTTTGCCCA 
GTAAACTCTT GTGCCAGGGA AACATCACTT GCAGAAAACG TTTGGCAAGA ACAACCTCAT 
TCTAAAATCT CTCTTTCTAT AGGTTCCAGT GTACCTTTCT CCATTTTTGA TGAATCTCTT 
CTTTCAGAAA AAAAGCCTGT CAGTCCTTCT GCAGCTCCCT CACGTGTTTT AGCCCAACGA 
AGACCTCTTG CAATTCTCAA AACTTCAGAA AGCATCACCT CAAATGAGGA TGTATCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAG CCCTTGAGTG AGGATGCTAT TATCACAGGT 
TTCAGGAATG TAACTATTTG TCCTAACCCA GAAGACACTT GTGACTTCGC TAGAGCAGCT 
CGTTTTGCAT CCACTCCTTT TCATGAAATA ATATCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TGCAGGAAGA GGATCTAGAT ATGAAGACCT CTGAGGACCA GCAAACTGCT 
TGTGGCACTA TCTACAATCC ACCTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGCCGTGAAG CCACACATTC CTCTGGGTTC TCCGGATCTT CTGCCTCAGT TACAAGCACC 
TCCTCCATCA AAAGTCTTCA AATTCCTGAG AAACTGGAGC TCACTAGTGA TACTGCAGAC 
AGCCCTACTC AGTCACCCTG GTGTCCACAG TATCGCAGAC AACTGGTAAA ATCCCTACCA 
GAGCTGAGTG TCTCTACAGA GTTTTTTGTA GAAGATAGAC CAATGCCTAG TTTGGAAATA 
GAGAAGGAAA TTGAATTAGA TAATGAGGAA TATTGCATTA GACAAGAATA CCTGGTATGT 
GAAGACTACA AATTATTCTG GGTGACACCA AGAAACACTG CCGAGTTAAC CATAATAAAG 
GTATCTTCTC ATCCTGTCCC ATGGGACTTC TATATCAACT TCAAGTTAAA GGAACGTTTA 
AATGAGGAGT GTGATAATTT GTGTAGCTGT TATCAATACC AAGATGGCTG TATCGTTTGG 
CATCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTTC AACACAGTGA ATTTATTACC 
CATGAAATGA CGGTATTGAT TATTTATAAC CTTTTGACAA TGGTGGAGAA ACTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGAGCCTGA TTCTTAGAAA CAGAATCCAC 
GATCCCTATG ATTTTAATAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGCACAGCT AGACGTTTTT AACCTCAGTG GCTTTCGGAC TGTACAAACC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCAT ACCAGGTAGA TCTGTTTGGT 
ATAGCAGATT TAGCACATTT ATTATTGTTC AAGGAGCACC TACAGGTCTT CTGGGATGGT 
TCCCTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTTTGC GGATTCTGAA TGCCAGTGAT GAGTCCACAG TGTCTGTTCT GGGGGAACTT 
GCAGCAGAAA TGAATGGGGT GTTTGACACC ACATTCCAAA GTCACCTGAA CAAAGCTTTT 
TGGAAAGTGG GGAAGTTAAT CAGTCCTGGG GCTTTCCTCT TCCAGCAAGA TAGGCAGTCA 
AGCCTCTCAC AGATTCCTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853463.1
CDS	242..3445
Translation	MAAMQKERGAPSDIMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQQESACNTTLQQQKRAFESEIRFYAGNDPLDVWDRYINWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYNDPRFLSLWLKLGHLCNEPLDMYSYLHNQGIGISLAQFYISWAEEYEARENFKKADLIFQEGIEQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVVGSSVPQRSTLAELKSKGKKTARAPISRVGGALKAPSQNRGLQNPVPQQMQSNTRIIVFDENTDEASRAEPSKPTVQPWIAPPVSRGKENELQAGPWNTGRPLEYRPRGNTASMTAVPSMLPSFTPYVEETAQQPVMTPCKIEPSINHILSTRKPGKEEGDLLQRVQSLQQESEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKERREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERANEQQEEKMPTKETAELPIASESQEISGMALSNFVCPVNSCARETSLAENVWQEQPHSKISLSIGSSVPFSIFDESLLSEKKPVSPSAAPSRVLAQRRPLAILKTSESITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFASTPFHEIISLKDLPSDPERLLQEEDLDMKTSEDQQTACGTIYNPPLSIKKLSPIIEDSREATHSSGFSGSSASVTSTSSIKSLQIPEKLELTSDTADSPTQSPWCPQYRRQLVKSLPELSVSTEFFVEDRPMPSLEIEKEIELDNEEYCIRQEYLVCEDYKLFWVTPRNTAELTIIKVSSHPVPWDFYINFKLKERLNEECDNLCSCYQYQDGCIVWHQYINCFTLQDLLQHSEFITHEMTVLIIYNLLTMVEKLHKAEIVHGDLSPRSLILRNRIHDPYDFNKNNQALKIVDFSYSVDLRAQLDVFNLSGFRTVQTLEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSLWKLSQNISELKDGELWNKFFLRILNASDESTVSVLGELAAEMNGVFDTTFQSHLNKAFWKVGKLISPGAFLFQQDRQSSLSQIPA

Target ORF information:

RefSeq Version	XM_024997695.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997695.1

ORF Insert Sequence:

ATGGCGGCGA TGCAGAAGGA AAGGGGTGCT CCGAGTGACA TCATGTCCTT GGAGGGAGAT 
GAATGGGAGC TGAGTAAAGA AAATGTCCAA CCTTTAAGGC AGGGGCGGAT CATGTCCACA 
CTTCAGGGAG CTCTGGCACA GCAAGAATCT GCCTGTAACA CTACTCTTCA ACAACAAAAA 
CGAGCATTTG AATCTGAAAT TCGATTTTAT GCTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGATATATTA ACTGGACAGA GCAAAACTAT CCTCAGGGCG GGAAGGAAAG CAATATGTCA 
ACATTACTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AACGATATTA TAATGATCCT 
CGATTTCTTA GTCTCTGGCT TAAGCTGGGG CATTTATGCA ATGAACCTTT GGATATGTAC 
AGTTATTTAC ATAACCAAGG AATTGGCATT TCACTTGCTC AGTTTTATAT CTCATGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGATC TGATATTTCA AGAAGGTATT 
GAACAGAAGG CTGAACCACT GGAAAGACTG CAGTCCCAGC ACAGACAATT CCAAGCTCGA 
GTGTCTCGGC AAACTCTGTT GGCACTTGAG AAAGAAGAGG AGGAGGAAGT TGTGGGATCT 
TCTGTACCAC AGCGAAGCAC ACTGGCTGAA CTAAAAAGCA AAGGGAAAAA GACAGCAAGA 
GCTCCAATCA GCCGTGTAGG GGGTGCTCTC AAGGCTCCAA GCCAGAACAG AGGTCTCCAA 
AATCCAGTTC CTCAACAGAT GCAAAGTAAT ACTAGAATTA TTGTTTTTGA TGAAAATACT 
GATGAGGCTT CTAGAGCAGA GCCATCTAAG CCTACAGTCC AGCCATGGAT AGCACCTCCT 
GTGTCCAGGG GCAAAGAGAA TGAGCTGCAA GCCGGCCCTT GGAACACAGG CAGGCCTTTG 
GAATACAGGC CTCGAGGCAA TACAGCGTCT ATGACAGCTG TACCCTCTAT GCTTCCAAGT 
TTTACTCCAT ATGTGGAAGA GACTGCACAA CAGCCAGTTA TGACACCATG TAAAATTGAA 
CCCAGTATAA ACCACATTCT AAGCACAAGA AAGCCTGGAA AGGAAGAAGG AGATCTCCTG 
CAGAGAGTTC AAAGTCTTCA GCAAGAGTCT GAGAAGAAGG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT GGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAGGTTTTC 
CGGAAGAAAT TAAAAGAGCG AAGAGAAGCT GAGCTACTGA CCAGTGCAGA GAAAAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG 
CAAGAAAGAG CGAATGAACA GCAAGAAGAA AAGATGCCTA CAAAGGAGAC AGCTGAACTG 
CCAATTGCTT CAGAGTCTCA GGAAATATCA GGAATGGCTC TGTCCAATTT TGTTTGCCCA 
GTAAACTCTT GTGCCAGGGA AACATCACTT GCAGAAAACG TTTGGCAAGA ACAACCTCAT 
TCTAAAATCT CTCTTTCTAT AGGTTCCAGT GTACCTTTCT CCATTTTTGA TGAATCTCTT 
CTTTCAGAAA AAAAGCCTGT CAGTCCTTCT GCAGCTCCCT CACGTGTTTT AGCCCAACGA 
AGACCTCTTG CAATTCTCAA AACTTCAGAA AGCATCACCT CAAATGAGGA TGTATCTCCA 
GATGTTTGTG ATGAATTTAC AGGAATTGAG CCCTTGAGTG AGGATGCTAT TATCACAGGT 
TTCAGGAATG TAACTATTTG TCCTAACCCA GAAGACACTT GTGACTTCGC TAGAGCAGCT 
CGTTTTGCAT CCACTCCTTT TCATGAAATA ATATCCTTGA AGGATCTCCC TTCTGATCCT 
GAGAGACTGT TGCAGGAAGA GGATCTAGAT ATGAAGACCT CTGAGGACCA GCAAACTGCT 
TGTGGCACTA TCTACAATCC ACCTCTCAGC ATCAAGAAGC TGAGCCCAAT TATTGAAGAC 
AGCCGTGAAG CCACACATTC CTCTGGGTTC TCCGGATCTT CTGCCTCAGT TACAAGCACC 
TCCTCCATCA AAAGTCTTCA AATTCCTGAG AAACTGGAGC TCACTAGTGA TACTGCAGAC 
AGCCCTACTC AGTCACCCTG GTGTCCACAG TATCGCAGAC AACTGGTAAA ATCCCTACCA 
GAGCTGAGTG TCTCTACAGA GTTTTTTGTA GAAGATAGAC CAATGCCTAG TTTGGAAATA 
GAGAAGGAAA TTGAATTAGA TAATGAGGAA TATTGCATTA GACAAGAATA CCTGGTATGT 
GAAGACTACA AATTATTCTG GGTGACACCA AGAAACACTG CCGAGTTAAC CATAATAAAG 
GTATCTTCTC ATCCTGTCCC ATGGGACTTC TATATCAACT TCAAGTTAAA GGAACGTTTA 
AATGAGGAGT GTGATAATTT GTGTAGCTGT TATCAATACC AAGATGGCTG TATCGTTTGG 
CATCAATATA TAAACTGCTT CACCCTTCAG GATCTTCTTC AACACAGTGA ATTTATTACC 
CATGAAATGA CGGTATTGAT TATTTATAAC CTTTTGACAA TGGTGGAGAA ACTACACAAA 
GCAGAAATAG TCCATGGTGA CTTGAGTCCA AGGAGCCTGA TTCTTAGAAA CAGAATCCAC 
GATCCCTATG ATTTTAATAA GAACAATCAA GCTTTGAAGA TAGTGGACTT TTCCTACAGT 
GTTGACCTTA GGGCACAGCT AGACGTTTTT AACCTCAGTG GCTTTCGGAC TGTACAAACC 
CTGGAAGGAC AAAAGATCCT GGCTAACTGT TCTTCTCCAT ACCAGGTAGA TCTGTTTGGT 
ATAGCAGATT TAGCACATTT ATTATTGTTC AAGGAGCACC TACAGGTCTT CTGGGATGGT 
TCCCTCTGGA AACTTAGCCA AAATATTTCT GAGCTAAAAG ATGGTGAATT GTGGAATAAA 
TTCTTTTTGC GGATTCTGAA TGCCAGTGAT GAGTCCACAG TGTCTGTTCT GGGGGAACTT 
GCAGCAGAAA TGAATGGGGT GTTTGACACC ACATTCCAAA GTCACCTGAA CAAAGCTTTT 
TGGAAAGTGG GGAAGTTAAT CAGTCCTGGG GCTTTCCTCT TCCAGCAAGA TAGGCAGTCA 
AGCCTCTCAC AGATTCCTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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BUB1B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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