bub1b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	bub1b
Entrez Gene ID	100125106
Full Name	BUB1B, mitotic checkpoint serine/threonine kinase
Synonyms	bubr1
General protein information	Preferred Names BUB1B, mitotic checkpoint serine/threonine kinase Names mitotic checkpoint serine/threonine-protein kinase BUB1 beta BUB1 mitotic checkpoint serine/threonine kinase B budding uninhibited by benzimidazoles 1 homolog beta
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012968790.2	XP_012824244.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
XM_031891202.1	XP_031747062.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Pathways from BioSystems

Homologies

Homo sapiens (human)	BUB1B	NP_001202.4
Pan troglodytes (chimpanzee)	BUB1B	XP_510299.3
Canis lupus familiaris (dog)	BUB1B	XP_850443.2
Macaca mulatta (Rhesus monkey)	BUB1B	XP_002804771.1
Gallus gallus (chicken)	BUB1B	NP_989734.1
Bos taurus (cattle)	BUB1B	NP_001138645.1
Arabidopsis thaliana (thale cress)	BUBR1	NP_565767.1
Oryza sativa (rice)	Os02g0193600	NP_001046171.1
Mus musculus (house mouse)	Bub1b	NP_033903.2
Rattus norvegicus (Norway rat)	Bub1b	XP_342495.4
Danio rerio (zebrafish)	bub1bb	XP_695384.3
Xenopus tropicalis (tropical clawed frog)	bub1b	NP_001096484.1

Gene Ontology

The following bub1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bub1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa30692	XM_012968790.2 Latest version!	Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa64464	XM_031891202.1 Latest version!	Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bub1b ( XM_012968790.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824244.1 Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA.

Price & Availability↑

CloneID	OXa30692
Clone ID Related Accession (Same CDS sequence)	XM_012968790.2
Accession Version	XM_012968790.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968790.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGCGCAAG CTGGCGATGA ATGGGAGCTA AGCAAAGAAA ATGTGCAACC CCTGCGACAG 
GGCAGAGTTA TGTCCACTCT GCAGGAGGTG CTCTCACAGC AGGAGAGTGC AAGCCACACT 
GCCATTCAGC AGCAGAAGCA AGCATTTGAG CTGGAACTGC GCTTCTATTC AGGTGATGAT 
CCACTAGATG TCTGGGACAG GTACATCAAG TGGGCTGAGC AGGCATTCCC TCAAGGAGGG 
AAAGAGAGCA ACTTGTGCCC ATTGCTGGAA AGAGCTGTTA AGATATTCCA TGAAGAACAG 
AGATATTATG ATGATCTGCG CTATCTTAGC ATTTGTCTCA AGTTTGCTAA TTTCTGCTCA 
GAGCCTCTGG ATTTGTACAG CTATTTGCAC AGCCAAAGAA TTGGGGTCTC TCATGCCCTA 
CTTTATATCA CTTGGGCCGA ACAGTTTGAA GCTCGAGGTA ATTTCAAGAA AGCTGACTCC 
ATATTTCAGC AGGGCATGCA ACACAAGGCT GAACCACTGG AAAAGCTAGA GATCCATCAT 
CGGCAGTTTC AGGCCCGAGT ATCCCGGCAA ATATTACAAG GAATTTCAGA GGGGCCAGAG 
GCAGAAGAAC CTGAAGTTCC AGAGCCACAG CGCAGCTCAC TGGCAGATCT AAAGTCCAGA 
GGGAAGAACA AAGTGAAAGC ACCCATTAAT CGAGTTGGAG ACTCGATCAA ACCACGTCCT 
CATGGCCTCA GCGTTCAAGC TGCTCCAACA CAGCAAATTC CAAGCCGCTC TCGCTTTGCT 
GTGTTTGATG AAAATGCAGC CTCAGCAGCC CCAGAGCTGC CCTCCTTGAC TCCACAGCAG 
TGGACTGCAC CACCCCCTGC ACGGTCTAAG GAAAATGAAC AGAAAGCTGG ACAATGGAAC 
TCTGGCAGGC CTTCTAGAAA CAGCCATCAG GCTGCTGCTG CCTCTGAACC GCCCCAAACT 
TTGCCCAGTT TCATGCCTTA TGTGGATGAA AGTGCTCAGC AGCAAACAGT AACTCCATGT 
AAAATAAACC CAGCAGTCAC TAGTGTCTTA AGCTCCCGCA AGCCAGGAAA AGAAGAGGAT 
CCTCTGCAAA GGGTACAGAA CAATTCCCAA GGAAAAGAGG AGAAGGTTAT GTACTGCAAG 
GATAAAGTTT ACGCTGGCGT GGAAGAGTTC TCCTTGGAAG AGATAAGGGC AGAAATCTAC 
TTGGCAAAAA TACGGAAGAA GAGAGAAGAC GACCTACAGG AGATTGCACT GAGGAGGCAG 
GAAATGGAGC GACAGATACA GGAGATGGAG AAGCAGCTAA AGGGATCATG TATTGAAAGC 
GAAGAGATCG TAAGTGATCA GGCCAAAACC AGTGGTGGCA CAAATGCCTC TATCTGCCTG 
TCACTCATTC ATAAAGTAAA GTCATACTGG AGGATGGAGA CCCTGCCCGT ATTTAATGAA 
ACAGGGAACA AGCCCAGCTT GCCAGGTTTG TCTCCAGCAC TTTCCTGTGA CTTGCCCTTC 
ACAATATTTG ATGAATCTGC AGAAGCTGCA CCTAGCATGA CTGACCAGAA GAGAATGGCT 
GCTCCTGTAC GTCGCCCACT TGCTGCTGTT CCTAAGTCCA TTGTTAAATC ACAAGAATCC 
CAACTAACCG ACACCCTGGA TGGAATTGAA CCCTTAAATG AAGAGGCAAT TGTGTGTGGC 
TCAGGTAAAA ACAAATCCTT ATTCCCTGAC CCAGAAGACA CTTGTGATTT TGTGAGAGCT 
GCCCATCTGG CGTCAACACC TTTCCATAGG GCTAGGGACC AATCAGGCGA ATGCTTACAG 
AGTGACTCGC TTGATCTGGA AAGGCTCCCG CTTCGAGAGA AGACACCTGT GTGCGAGGAG 
TCTTATAGAC AAGAACTCTG CATAAAGAAA CTAAGCCCCA TTTTAGAAGC AAGCCAAGAA 
GATACACGCA CATCAGTCTC CTCTGTGTCC TCAATCTCGA GCACAACCAG CATGTTCACT 
TCCAGGCCAA TTCCAGAGAA GCTCGAGCTG GCTACTCAGA TTACAGGGGT GTATGAGTCT 
GAGCCAACCA CAGAAGAGCT TCCACAAGCA GAAGAACTTG CAGAACTGCA TAGGCAGTTG 
CTGGAATCTC TGCCAGAGCT GCTGGTCTCT CCAGAGATCC AACATGAGGT TGGAACGATG 
CCTGATATGA AAGAACAAGG AGAGGTGGTG CTGGGTTGTG AAACTTACGG TCTAAAAAAT 
GAAGTCATCC TTCATCCTAA CAGCAAGTTG TTTATGGGTG CCCCTGTGGA TTGGGATATG 
GGAGACATGA AGGCATTTGC TCTAAAGGTG GATTATCAGC CACTTCCATG GGATTTCTAT 
GTTACAATGC AGCTGAAGGA GCGCCTTGGA GATCTATTTG AAACATTTTT CATTGAACAA 
ACTAACTGCT TCCTCTACCA GAATGGGTGC ATCAGTCTCT ACAAGGATAT CAACCGTTTC 
AGTATTCAAG AGATCCTTTT GGATTCTGAA GAACATATCA AAGAAGTAAT TGTGTTGGTA 
ACATACAACT TGTTGAGCCT GGTGGAAAAG CTTCACTCAG TGGAAATTGT TCATGGAGAT 
CTGAGGCCAG AGACTCTACT TCTGGATGAC AAGATTTTTG ATTTGTCGTC ATCTCTGGAA 
CTGGAGGGTT TATTTAAGCT GGTAGATTTT TCCCATAGCG TGGATCTGAA ATTATACCCC 
ATAATGAGCT CATTACGTGG CTTCCCCATT GCTCAAACTG AAAGTGGTCA ACAATTTCTC 
AGTCCTCAGT CCTCCCCTTA TCAGGTGGAC CTTCTTGGCA TTGCAGACCT GGTTCACCTA 
ATGATTTTCA GGAAACCATT GCAGCTGCAC CAGGAAAATT CTGTTTGGAT GATTTGTAAA 
GAGGTTCCTA GACTGCGTGG TGGAAATCTC TGGAACAAAT TCTTTACAAA GATCCTAAAT 
GCAGAAGGCC CAAGCACACG TGTTCTGGGG GAGCTAAAGG GAGAAATGAT GGGACAATTT 
GACAGCTGCT TTCAAGACAA ACTTTGCAAC TATTTCATCC AGCTGGAGAT GAGACTGAAC 
CCGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824244.1
CDS	152..3220
Translation	MAQAGDEWELSKENVQPLRQGRVMSTLQEVLSQQESASHTAIQQQKQAFELELRFYSGDDPLDVWDRYIKWAEQAFPQGGKESNLCPLLERAVKIFHEEQRYYDDLRYLSICLKFANFCSEPLDLYSYLHSQRIGVSHALLYITWAEQFEARGNFKKADSIFQQGMQHKAEPLEKLEIHHRQFQARVSRQILQGISEGPEAEEPEVPEPQRSSLADLKSRGKNKVKAPINRVGDSIKPRPHGLSVQAAPTQQIPSRSRFAVFDENAASAAPELPSLTPQQWTAPPPARSKENEQKAGQWNSGRPSRNSHQAAAASEPPQTLPSFMPYVDESAQQQTVTPCKINPAVTSVLSSRKPGKEEDPLQRVQNNSQGKEEKVMYCKDKVYAGVEEFSLEEIRAEIYLAKIRKKREDDLQEIALRRQEMERQIQEMEKQLKGSCIESEEIVSDQAKTSGGTNASICLSLIHKVKSYWRMETLPVFNETGNKPSLPGLSPALSCDLPFTIFDESAEAAPSMTDQKRMAAPVRRPLAAVPKSIVKSQESQLTDTLDGIEPLNEEAIVCGSGKNKSLFPDPEDTCDFVRAAHLASTPFHRARDQSGECLQSDSLDLERLPLREKTPVCEESYRQELCIKKLSPILEASQEDTRTSVSSVSSISSTTSMFTSRPIPEKLELATQITGVYESEPTTEELPQAEELAELHRQLLESLPELLVSPEIQHEVGTMPDMKEQGEVVLGCETYGLKNEVILHPNSKLFMGAPVDWDMGDMKAFALKVDYQPLPWDFYVTMQLKERLGDLFETFFIEQTNCFLYQNGCISLYKDINRFSIQEILLDSEEHIKEVIVLVTYNLLSLVEKLHSVEIVHGDLRPETLLLDDKIFDLSSSLELEGLFKLVDFSHSVDLKLYPIMSSLRGFPIAQTESGQQFLSPQSSPYQVDLLGIADLVHLMIFRKPLQLHQENSVWMICKEVPRLRGGNLWNKFFTKILNAEGPSTRVLGELKGEMMGQFDSCFQDKLCNYFIQLEMRLNPL

Target ORF information:

RefSeq Version	XM_012968790.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968790.2

ORF Insert Sequence:

ATGGCGCAAG CTGGCGATGA ATGGGAGCTA AGCAAAGAAA ATGTGCAACC CCTGCGACAG 
GGCAGAGTTA TGTCCACTCT GCAGGAGGTG CTCTCACAGC AGGAGAGTGC AAGCCACACT 
GCCATTCAGC AGCAGAAGCA AGCATTTGAG CTGGAACTGC GCTTCTATTC AGGTGATGAT 
CCACTAGATG TCTGGGACAG GTACATCAAG TGGGCTGAGC AGGCATTCCC TCAAGGAGGG 
AAAGAGAGCA ACTTGTGCCC ATTGCTGGAA AGAGCTGTTA AGATATTCCA TGAAGAACAG 
AGATATTATG ATGATCTGCG CTATCTTAGC ATTTGTCTCA AGTTTGCTAA TTTCTGCTCA 
GAGCCTCTGG ATTTGTACAG CTATTTGCAC AGCCAAAGAA TTGGGGTCTC TCATGCCCTA 
CTTTATATCA CTTGGGCCGA ACAGTTTGAA GCTCGAGGTA ATTTCAAGAA AGCTGACTCC 
ATATTTCAGC AGGGCATGCA ACACAAGGCT GAACCACTGG AAAAGCTAGA GATCCATCAT 
CGGCAGTTTC AGGCCCGAGT ATCCCGGCAA ATATTACAAG GAATTTCAGA GGGGCCAGAG 
GCAGAAGAAC CTGAAGTTCC AGAGCCACAG CGCAGCTCAC TGGCAGATCT AAAGTCCAGA 
GGGAAGAACA AAGTGAAAGC ACCCATTAAT CGAGTTGGAG ACTCGATCAA ACCACGTCCT 
CATGGCCTCA GCGTTCAAGC TGCTCCAACA CAGCAAATTC CAAGCCGCTC TCGCTTTGCT 
GTGTTTGATG AAAATGCAGC CTCAGCAGCC CCAGAGCTGC CCTCCTTGAC TCCACAGCAG 
TGGACTGCAC CACCCCCTGC ACGGTCTAAG GAAAATGAAC AGAAAGCTGG ACAATGGAAC 
TCTGGCAGGC CTTCTAGAAA CAGCCATCAG GCTGCTGCTG CCTCTGAACC GCCCCAAACT 
TTGCCCAGTT TCATGCCTTA TGTGGATGAA AGTGCTCAGC AGCAAACAGT AACTCCATGT 
AAAATAAACC CAGCAGTCAC TAGTGTCTTA AGCTCCCGCA AGCCAGGAAA AGAAGAGGAT 
CCTCTGCAAA GGGTACAGAA CAATTCCCAA GGAAAAGAGG AGAAGGTTAT GTACTGCAAG 
GATAAAGTTT ACGCTGGCGT GGAAGAGTTC TCCTTGGAAG AGATAAGGGC AGAAATCTAC 
TTGGCAAAAA TACGGAAGAA GAGAGAAGAC GACCTACAGG AGATTGCACT GAGGAGGCAG 
GAAATGGAGC GACAGATACA GGAGATGGAG AAGCAGCTAA AGGGATCATG TATTGAAAGC 
GAAGAGATCG TAAGTGATCA GGCCAAAACC AGTGGTGGCA CAAATGCCTC TATCTGCCTG 
TCACTCATTC ATAAAGTAAA GTCATACTGG AGGATGGAGA CCCTGCCCGT ATTTAATGAA 
ACAGGGAACA AGCCCAGCTT GCCAGGTTTG TCTCCAGCAC TTTCCTGTGA CTTGCCCTTC 
ACAATATTTG ATGAATCTGC AGAAGCTGCA CCTAGCATGA CTGACCAGAA GAGAATGGCT 
GCTCCTGTAC GTCGCCCACT TGCTGCTGTT CCTAAGTCCA TTGTTAAATC ACAAGAATCC 
CAACTAACCG ACACCCTGGA TGGAATTGAA CCCTTAAATG AAGAGGCAAT TGTGTGTGGC 
TCAGGTAAAA ACAAATCCTT ATTCCCTGAC CCAGAAGACA CTTGTGATTT TGTGAGAGCT 
GCCCATCTGG CGTCAACACC TTTCCATAGG GCTAGGGACC AATCAGGCGA ATGCTTACAG 
AGTGACTCGC TTGATCTGGA AAGGCTCCCG CTTCGAGAGA AGACACCTGT GTGCGAGGAG 
TCTTATAGAC AAGAACTCTG CATAAAGAAA CTAAGCCCCA TTTTAGAAGC AAGCCAAGAA 
GATACACGCA CATCAGTCTC CTCTGTGTCC TCAATCTCGA GCACAACCAG CATGTTCACT 
TCCAGGCCAA TTCCAGAGAA GCTCGAGCTG GCTACTCAGA TTACAGGGGT GTATGAGTCT 
GAGCCAACCA CAGAAGAGCT TCCACAAGCA GAAGAACTTG CAGAACTGCA TAGGCAGTTG 
CTGGAATCTC TGCCAGAGCT GCTGGTCTCT CCAGAGATCC AACATGAGGT TGGAACGATG 
CCTGATATGA AAGAACAAGG AGAGGTGGTG CTGGGTTGTG AAACTTACGG TCTAAAAAAT 
GAAGTCATCC TTCATCCTAA CAGCAAGTTG TTTATGGGTG CCCCTGTGGA TTGGGATATG 
GGAGACATGA AGGCATTTGC TCTAAAGGTG GATTATCAGC CACTTCCATG GGATTTCTAT 
GTTACAATGC AGCTGAAGGA GCGCCTTGGA GATCTATTTG AAACATTTTT CATTGAACAA 
ACTAACTGCT TCCTCTACCA GAATGGGTGC ATCAGTCTCT ACAAGGATAT CAACCGTTTC 
AGTATTCAAG AGATCCTTTT GGATTCTGAA GAACATATCA AAGAAGTAAT TGTGTTGGTA 
ACATACAACT TGTTGAGCCT GGTGGAAAAG CTTCACTCAG TGGAAATTGT TCATGGAGAT 
CTGAGGCCAG AGACTCTACT TCTGGATGAC AAGATTTTTG ATTTGTCGTC ATCTCTGGAA 
CTGGAGGGTT TATTTAAGCT GGTAGATTTT TCCCATAGCG TGGATCTGAA ATTATACCCC 
ATAATGAGCT CATTACGTGG CTTCCCCATT GCTCAAACTG AAAGTGGTCA ACAATTTCTC 
AGTCCTCAGT CCTCCCCTTA TCAGGTGGAC CTTCTTGGCA TTGCAGACCT GGTTCACCTA 
ATGATTTTCA GGAAACCATT GCAGCTGCAC CAGGAAAATT CTGTTTGGAT GATTTGTAAA 
GAGGTTCCTA GACTGCGTGG TGGAAATCTC TGGAACAAAT TCTTTACAAA GATCCTAAAT 
GCAGAAGGCC CAAGCACACG TGTTCTGGGG GAGCTAAAGG GAGAAATGAT GGGACAATTT 
GACAGCTGCT TTCAAGACAA ACTTTGCAAC TATTTCATCC AGCTGGAGAT GAGACTGAAC 
CCGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1b ( XM_031891202.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747062.1 Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA.

Price & Availability↑

CloneID	OXa64464
Clone ID Related Accession (Same CDS sequence)	XM_031891202.1
Accession Version	XM_031891202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAAG CTGGCGATGA ATGGGAGCTA AGCAAAGAAA ATGTGCAACC CCTGCGACAG 
GGCAGAGTTA TGTCCACTCT GCAGGAGGTG CTCTCACAGC AGGAGAGTGC AAGCCACACT 
GCCATTCAGC AGCAGAAGCA AGCATTTGAG CTGGAACTGC GCTTCTATTC AGGTGATGAT 
CCACTAGATG TCTGGGACAG GTACATCAAG TGGGCTGAGC AGGCATTCCC TCAAGGAGGG 
AAAGAGAGCA ACTTGTGCCC ATTGCTGGAA AGAGCTGTTA AGATATTCCA TGAAGAACAG 
CGATATTATG ATGATCTGCG CTATCTTAGC ATTTGTCTCA AGTTTGCTAA TTTCTGCTCA 
GAGCCTCTGG ATTTGTACAG CTATTTGCAC AGCCAAAGAA TTGGGGTCTC TCATGCCCTA 
CTTTATATCA CTTGGGCCGA ACAGTTTGAA GCTCGAGGTA ATTTCAAGAA AGCTGACTCC 
ATATTTCAGC AGGGCATGCA ACACAAGGCT GAACCACTGG AAAAGCTAGA GATCCATCAT 
CGGCAGTTTC AGGCCCGAGT ATCCCGGCAA ATATTACAAG GAATTTCAGA GGGGCCAGAG 
GCAGAAGAAC CTGAAGTTCC AGAGCCACAG CGCAGCTCAC TGGCAGATCT AAAGTCCAGA 
GGGAAGAACA AAGTGAAAGC ACCCATTAAT CGAGTTGGAG ACTCGATCAA ACCACGTCCT 
CATGGCCTCA GCGTTCAAGC TGCTCCAACA CAGCAAATTC CAAGCCGCTC TCGCTTTGCT 
GTGTTTGATG AAAATGCAGC CTCAGCAGCC CCAGAGCTGC CCTCCTTGAC TCCACAGCAG 
TGGACTGCAC CACCCCCTGC ACGGTCTAAG GAAAATGAAC AGAAAGCTGG ACAATGGAAC 
TCTGGCAGGC CTTCTAGAAA CAGCCATCAG GCTGCTGCTG CCTCTGAACC GCCCCAAACT 
TTGCCCAGTT TCATGCCTTA TGTGGATGAA AGTGCTCAGC AGCAAACAGT AACTCCATGT 
AAAATAAACC CAGCAGTCAC TAGTGTCTTA AGCTCCCGCA AGCCAGGAAA AGAAGAGGAT 
CCTCTGCAAA GGGTACAGAA CAATTCCCAA GGAAAAGAGG AGAAGGTTAT GTACTGCAAG 
GATAAAGTTT ACGCTGGCGT GGAAGAGTTC TCCTTGGAAG AGATAAGGGC AGAAATCTAC 
TTGGCAAAAA TACGGAAGAA GAGAGAAGAC GACCTACAGG AGATTGCACT GAGGAGGCAG 
GAAATGGAGC GACAGATACA GGAGATGGAG AAGCAGCTAA AGGGATCATG TATTGAAAGC 
GAAGAGATCG TAAGTGATCA GCCTGTACAG AATGTTGAAC CTATTATTGC CCCATGTAAT 
TCCAATACAA ATGAGAGTCT GTGTGCATCT CAGCAGGCAC CAGTCATGGA ATTTCCACAT 
TGCTTTGAAA TGGCTGATGC TGCTCCAACA CTGCCAAGGA TGGAGACCCT GCCCGTATTT 
AATGAAACCG GGAACAAGCC CAGCTTGCCA GGTTTGTCTC CAGCACTTTC CTGTGACTTG 
CCCTTCACAA TATTTGATGA ATCTGCAGAA GCTGCACCTA GCATGACTGA CCAGAAGAGA 
ATGGCTGCTC CTGTACGTCG CCCACTTGCT GCTGTTCCTA AGTCCATTGT TAAATCACAA 
GAATCCCAAC TAACCGACAC CCTGGATGGA ACTGAACCCT TAAATGAAGA GGCAATTGTG 
TGTGGCTCAG GTAAAAACAA ATCCTTATTC CCTGACCCAG AAGACACTTG TGATTTTGTG 
AGAGCTGCCC ATCTGGCGTC AACACCTTTC CATAGGGCTA GGGACCAATC AGGCGAATGC 
TTACAGAGTG ACTCGCTTGA TCTGGAAAGG CTCCCGCTTC GAGAGAAGAC ACCTGTGTGC 
GAGGAGTCTT ATAGACAAGA ACTCTGCATA AAGAAACTAA GCCCCATTTT AGAAGCAAGC 
CAAGAAGATA CACGCACATC AGTCTCCTCT GTGTCCTCAA TCTCGAGCAC AACCAGCATG 
TTCACTTCCA GGCCAATTCC AGAGAAGCTC GAGCTGGCTA CTCAGATTAC AGGGGTGTAT 
GAGTCTGAGC CAACCACAGA AGAGCTTCCA CAAGCAGAAG AACTTGCAGA ACTGCATAGG 
CAGTTGCTGG AATCTCTGCC AGAGCTGCTG GTCTCTCCAG AGATCCAACA TGAGGTTGGA 
ACGATGCCTG ATATGAAAGA ACAAGGAGAG GTGGTGCTGG GTTGTGAAAC TTACGGTCTA 
AAAAATGAAG TCATCCTTCA TCCTAACAGC AAGTTGTTTA TGGGTGCCCC TGTGGATTGG 
GATATGGGAG ACATGAAGGC ATTTGCTCTA AAGGTGGATT ATCAGCCACT TCCATGGGAT 
TTCTATGTTA CAATGCAGCT GAAGGAGCGC CTTGGAGATC TATTTGAAAC ATTTTTCATT 
GAACAAACTA ACTGCTTCCT CTACCAGAAT GGGTGCATCA GTCTCTACAA GGATATCAAC 
CGTTTCAGTA TTCAAGAGAT CCTTTTGGAT TCTGAAGAAC ATATCAAAGA AGTAATTGTG 
TTGGTAACAT ACAACTTGTT GAGCCTGGTG GAAAAGCTTC ACTCAGTGGA AATTGTTCAT 
GGAGATCTGA GGCCAGAGAC TCTACTTCTG GATGACAAGA TTTTTGATTT GTCGTCATCT 
CTGGAACTGA AGGGTTTATT TAAGCTGGTA GATTTTTCCC ATAGCGTGGA TCTGAAATTA 
TACCCCATAA TGAGCTCATT ACGTGGCTTC CCCATTGCTC AAACTGAAAG TGGTCAACAA 
TTTCTCAGTC CTCAGTCCTC CCCTTATCAG GTGGACCTTC TTGGCATTGC AGACCTGGTT 
CACCTAATGA TTTTCAGGAA ACCATTGCAG CTGCACCAGG AAAATTCTGT TTGGATGATT 
TGTAAAGAGG TTCCTAGACT GCGTGGTGGA AATCTCTGGA ACAAATTCTT TACAAAGATC 
CTAAATGCAG AAGGCCCAAG CACACGTGTT CTGGGGGAGC TAAAGGGAGA AATGATGGGA 
CAATTTGACA GCTGCTTTCA AGACAAACTT TGCAACTATT TCATCCAGCT GGAGATGAGA 
CTGAACCCGT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747062.1
CDS	150..3284
Translation	MAQAGDEWELSKENVQPLRQGRVMSTLQEVLSQQESASHTAIQQQKQAFELELRFYSGDDPLDVWDRYIKWAEQAFPQGGKESNLCPLLERAVKIFHEEQRYYDDLRYLSICLKFANFCSEPLDLYSYLHSQRIGVSHALLYITWAEQFEARGNFKKADSIFQQGMQHKAEPLEKLEIHHRQFQARVSRQILQGISEGPEAEEPEVPEPQRSSLADLKSRGKNKVKAPINRVGDSIKPRPHGLSVQAAPTQQIPSRSRFAVFDENAASAAPELPSLTPQQWTAPPPARSKENEQKAGQWNSGRPSRNSHQAAAASEPPQTLPSFMPYVDESAQQQTVTPCKINPAVTSVLSSRKPGKEEDPLQRVQNNSQGKEEKVMYCKDKVYAGVEEFSLEEIRAEIYLAKIRKKREDDLQEIALRRQEMERQIQEMEKQLKGSCIESEEIVSDQPVQNVEPIIAPCNSNTNESLCASQQAPVMEFPHCFEMADAAPTLPRMETLPVFNETGNKPSLPGLSPALSCDLPFTIFDESAEAAPSMTDQKRMAAPVRRPLAAVPKSIVKSQESQLTDTLDGTEPLNEEAIVCGSGKNKSLFPDPEDTCDFVRAAHLASTPFHRARDQSGECLQSDSLDLERLPLREKTPVCEESYRQELCIKKLSPILEASQEDTRTSVSSVSSISSTTSMFTSRPIPEKLELATQITGVYESEPTTEELPQAEELAELHRQLLESLPELLVSPEIQHEVGTMPDMKEQGEVVLGCETYGLKNEVILHPNSKLFMGAPVDWDMGDMKAFALKVDYQPLPWDFYVTMQLKERLGDLFETFFIEQTNCFLYQNGCISLYKDINRFSIQEILLDSEEHIKEVIVLVTYNLLSLVEKLHSVEIVHGDLRPETLLLDDKIFDLSSSLELKGLFKLVDFSHSVDLKLYPIMSSLRGFPIAQTESGQQFLSPQSSPYQVDLLGIADLVHLMIFRKPLQLHQENSVWMICKEVPRLRGGNLWNKFFTKILNAEGPSTRVLGELKGEMMGQFDSCFQDKLCNYFIQLEMRLNPL

Target ORF information:

RefSeq Version	XM_031891202.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis BUB1B, mitotic checkpoint serine/threonine kinase (bub1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891202.1

ORF Insert Sequence:

ATGGCGCAAG CTGGCGATGA ATGGGAGCTA AGCAAAGAAA ATGTGCAACC CCTGCGACAG 
GGCAGAGTTA TGTCCACTCT GCAGGAGGTG CTCTCACAGC AGGAGAGTGC AAGCCACACT 
GCCATTCAGC AGCAGAAGCA AGCATTTGAG CTGGAACTGC GCTTCTATTC AGGTGATGAT 
CCACTAGATG TCTGGGACAG GTACATCAAG TGGGCTGAGC AGGCATTCCC TCAAGGAGGG 
AAAGAGAGCA ACTTGTGCCC ATTGCTGGAA AGAGCTGTTA AGATATTCCA TGAAGAACAG 
CGATATTATG ATGATCTGCG CTATCTTAGC ATTTGTCTCA AGTTTGCTAA TTTCTGCTCA 
GAGCCTCTGG ATTTGTACAG CTATTTGCAC AGCCAAAGAA TTGGGGTCTC TCATGCCCTA 
CTTTATATCA CTTGGGCCGA ACAGTTTGAA GCTCGAGGTA ATTTCAAGAA AGCTGACTCC 
ATATTTCAGC AGGGCATGCA ACACAAGGCT GAACCACTGG AAAAGCTAGA GATCCATCAT 
CGGCAGTTTC AGGCCCGAGT ATCCCGGCAA ATATTACAAG GAATTTCAGA GGGGCCAGAG 
GCAGAAGAAC CTGAAGTTCC AGAGCCACAG CGCAGCTCAC TGGCAGATCT AAAGTCCAGA 
GGGAAGAACA AAGTGAAAGC ACCCATTAAT CGAGTTGGAG ACTCGATCAA ACCACGTCCT 
CATGGCCTCA GCGTTCAAGC TGCTCCAACA CAGCAAATTC CAAGCCGCTC TCGCTTTGCT 
GTGTTTGATG AAAATGCAGC CTCAGCAGCC CCAGAGCTGC CCTCCTTGAC TCCACAGCAG 
TGGACTGCAC CACCCCCTGC ACGGTCTAAG GAAAATGAAC AGAAAGCTGG ACAATGGAAC 
TCTGGCAGGC CTTCTAGAAA CAGCCATCAG GCTGCTGCTG CCTCTGAACC GCCCCAAACT 
TTGCCCAGTT TCATGCCTTA TGTGGATGAA AGTGCTCAGC AGCAAACAGT AACTCCATGT 
AAAATAAACC CAGCAGTCAC TAGTGTCTTA AGCTCCCGCA AGCCAGGAAA AGAAGAGGAT 
CCTCTGCAAA GGGTACAGAA CAATTCCCAA GGAAAAGAGG AGAAGGTTAT GTACTGCAAG 
GATAAAGTTT ACGCTGGCGT GGAAGAGTTC TCCTTGGAAG AGATAAGGGC AGAAATCTAC 
TTGGCAAAAA TACGGAAGAA GAGAGAAGAC GACCTACAGG AGATTGCACT GAGGAGGCAG 
GAAATGGAGC GACAGATACA GGAGATGGAG AAGCAGCTAA AGGGATCATG TATTGAAAGC 
GAAGAGATCG TAAGTGATCA GCCTGTACAG AATGTTGAAC CTATTATTGC CCCATGTAAT 
TCCAATACAA ATGAGAGTCT GTGTGCATCT CAGCAGGCAC CAGTCATGGA ATTTCCACAT 
TGCTTTGAAA TGGCTGATGC TGCTCCAACA CTGCCAAGGA TGGAGACCCT GCCCGTATTT 
AATGAAACCG GGAACAAGCC CAGCTTGCCA GGTTTGTCTC CAGCACTTTC CTGTGACTTG 
CCCTTCACAA TATTTGATGA ATCTGCAGAA GCTGCACCTA GCATGACTGA CCAGAAGAGA 
ATGGCTGCTC CTGTACGTCG CCCACTTGCT GCTGTTCCTA AGTCCATTGT TAAATCACAA 
GAATCCCAAC TAACCGACAC CCTGGATGGA ACTGAACCCT TAAATGAAGA GGCAATTGTG 
TGTGGCTCAG GTAAAAACAA ATCCTTATTC CCTGACCCAG AAGACACTTG TGATTTTGTG 
AGAGCTGCCC ATCTGGCGTC AACACCTTTC CATAGGGCTA GGGACCAATC AGGCGAATGC 
TTACAGAGTG ACTCGCTTGA TCTGGAAAGG CTCCCGCTTC GAGAGAAGAC ACCTGTGTGC 
GAGGAGTCTT ATAGACAAGA ACTCTGCATA AAGAAACTAA GCCCCATTTT AGAAGCAAGC 
CAAGAAGATA CACGCACATC AGTCTCCTCT GTGTCCTCAA TCTCGAGCAC AACCAGCATG 
TTCACTTCCA GGCCAATTCC AGAGAAGCTC GAGCTGGCTA CTCAGATTAC AGGGGTGTAT 
GAGTCTGAGC CAACCACAGA AGAGCTTCCA CAAGCAGAAG AACTTGCAGA ACTGCATAGG 
CAGTTGCTGG AATCTCTGCC AGAGCTGCTG GTCTCTCCAG AGATCCAACA TGAGGTTGGA 
ACGATGCCTG ATATGAAAGA ACAAGGAGAG GTGGTGCTGG GTTGTGAAAC TTACGGTCTA 
AAAAATGAAG TCATCCTTCA TCCTAACAGC AAGTTGTTTA TGGGTGCCCC TGTGGATTGG 
GATATGGGAG ACATGAAGGC ATTTGCTCTA AAGGTGGATT ATCAGCCACT TCCATGGGAT 
TTCTATGTTA CAATGCAGCT GAAGGAGCGC CTTGGAGATC TATTTGAAAC ATTTTTCATT 
GAACAAACTA ACTGCTTCCT CTACCAGAAT GGGTGCATCA GTCTCTACAA GGATATCAAC 
CGTTTCAGTA TTCAAGAGAT CCTTTTGGAT TCTGAAGAAC ATATCAAAGA AGTAATTGTG 
TTGGTAACAT ACAACTTGTT GAGCCTGGTG GAAAAGCTTC ACTCAGTGGA AATTGTTCAT 
GGAGATCTGA GGCCAGAGAC TCTACTTCTG GATGACAAGA TTTTTGATTT GTCGTCATCT 
CTGGAACTGA AGGGTTTATT TAAGCTGGTA GATTTTTCCC ATAGCGTGGA TCTGAAATTA 
TACCCCATAA TGAGCTCATT ACGTGGCTTC CCCATTGCTC AAACTGAAAG TGGTCAACAA 
TTTCTCAGTC CTCAGTCCTC CCCTTATCAG GTGGACCTTC TTGGCATTGC AGACCTGGTT 
CACCTAATGA TTTTCAGGAA ACCATTGCAG CTGCACCAGG AAAATTCTGT TTGGATGATT 
TGTAAAGAGG TTCCTAGACT GCGTGGTGGA AATCTCTGGA ACAAATTCTT TACAAAGATC 
CTAAATGCAG AAGGCCCAAG CACACGTGTT CTGGGGGAGC TAAAGGGAGA AATGATGGGA 
CAATTTGACA GCTGCTTTCA AGACAAACTT TGCAACTATT TCATCCAGCT GGAGATGAGA 
CTGAACCCGT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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bub1b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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