TRMT12 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMT12
Entrez Gene ID	516516
Full Name	tRNA methyltransferase 12 homolog
General protein information	Preferred Names tRNA methyltransferase 12 homolog Names tRNA wybutosine-synthesizing protein 2 homolog alpha-amino-alpha-carboxypropyl transferase TYW2 tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase tRNA-yW-synthesizing protein 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010811956.2	XP_010810258.1	tRNA wybutosine-synthesizing protein 2 homolog isoform X1
XM_005215368.3	XP_005215425.1	tRNA wybutosine-synthesizing protein 2 homolog isoform X1
NM_001024552.2	NP_001019723.2	tRNA wybutosine-synthesizing protein 2 homolog
XM_005215366.3	XP_005215423.1	tRNA wybutosine-synthesizing protein 2 homolog isoform X1
XM_005215367.3	XP_005215424.1	tRNA wybutosine-synthesizing protein 2 homolog isoform X1
NM_001024552.1	NP_001019723.1	tRNA wybutosine-synthesizing protein 2 homolog

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	TRMT12	XP_001101713.2
Xenopus tropicalis (tropical clawed frog)	trmt12	XP_004916821.1
Danio rerio (zebrafish)	LOC100535404	XP_003199977.1
Homo sapiens (human)	TRMT12	NP_060426.2
Pan troglodytes (chimpanzee)	TRMT12	XP_519945.2
Mus musculus (house mouse)	Trmt12	NP_080918.2
Rattus norvegicus (Norway rat)	Trmt12	NP_001116448.1
Bos taurus (cattle)	TRMT12	NP_001019723.1
Danio rerio (zebrafish)	zgc:153361	NP_001070632.1
Arabidopsis thaliana (thale cress)	AT4G04670	NP_567268.2
Canis lupus familiaris (dog)	TRMT12	XP_005627978.1
Gallus gallus (chicken)	TRMT12	XP_420244.4

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ESTs.
Casey OM, Fitzpatrick R, McInerney JO, Morris DG, Powell R, Sreenan JM
Biochimica et biophysica acta1679(1)10-7(2004 Jul)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TRMT12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa25891	NM_001024552.2 Latest version!	Bos taurus tRNA methyltransferase 12 homolog (TRMT12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa41536	NM_001024552.1 Latest version!	Bos taurus tRNA methyltransferase 12 homolog (TRMT12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa25891	XM_005215366.3 Latest version!	Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa25891	XM_005215367.3 Latest version!	Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa25891	XM_005215368.3 Latest version!	Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa25891	XM_010811956.2 Latest version!	Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMT12 ( NM_001024552.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001019723.2 Bos taurus tRNA methyltransferase 12 homolog (TRMT12), mRNA.

Price & Availability↑

CloneID	OBa25891
Clone ID Related Accession (Same CDS sequence)	XM_010811956.2 , XM_005215368.3 , NM_001024552.2 , XM_005215366.3 , XM_005215367.3
Accession Version	NM_001024552.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1545321600000
Organism	Bos taurus(cattle)
Product	tRNA wybutosine-synthesizing protein 2 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH150787.1, DV800851.1, BU917308.1, DV803609.1 and EV745924.1. On Jan 24, 2018 this sequence version replaced NM_001024552.1. COMPLETENESS: complete on the 3' end.

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019723.2
CDS	27..1415
Translation	MRGCAEAKQREFRGRSRRDCGASNMEGAGGKPTAVVAVVTEPRFTQRYREYLEKHKLLDRQHRVKKLRDGTVALPVLREALLEQHLRELRNRVAPGSTCVPTQLLDPVPSKKAQSYSPAQRLCLEVSRWVEGRGVTWSAELEADLPRSWQRHGDLLLLSEDCFQAKQWRHLEPELWETVASALGAQRLAKRGRVSPDSTRTPAVSLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLPCAGEVLVDLYAGIGYFTLPFLVHAEAAFVHACEWNPHAVVALRNNLELNGVADRCQIHFGDNRKLKLSNVADRVNLGLIPSSEEGWPVACRVLKQDAGGILHIHQNVESFPGKTLQPPGSSEMEEHWPSPHQIISNQLNNGATSDSRRKTLSVATKPEWQRWAKAAETRIATLLHQVHGKRWKTQILHIQPVKSYAPHVDHIVLDLECRPCHLVG

Target ORF information:

RefSeq Version	NM_001024552.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 12 homolog (TRMT12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024552.2

ORF Insert Sequence:

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT12 ( NM_001024552.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001019723.1 Bos taurus tRNA methyltransferase 12 homolog (TRMT12), mRNA.

Price & Availability↑

CloneID	OBa41536
Clone ID Related Accession (Same CDS sequence)	NM_001024552.1
Accession Version	NM_001024552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508515200000
Organism	Bos taurus(cattle)
Product	tRNA wybutosine-synthesizing protein 2 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT021732.1. On May 29, 2005 this sequence version replaced XM_594671.1. ##Evidence-Data-START## Transcript exon combination :: BT021732.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAAGGGG CAGGCGGGAA GCCCACGGCT GTTGTTGCAG TTGTGACTGA GCCTCGATTT 
ACTCAGCGAT ACAGGGAATA TCTCGAGAAG CACAAACTCC TGGATAGACA GCACCGTGTG 
AAAAAGCTGC GGGATGGCAC CGTGGCGCTA CCGGTTCTGA GAGAGGCCCT CCTTGAGCAG 
CATCTGCGAG AGCTGAGGAA TCGTGTGGCT CCAGGCAGCA CCTGTGTGCC GACGCAGCTC 
CTGGATCCTG TTCCTTCAAA GAAGGCCCAG AGTTACTCAC CTGCCCAAAG GCTGTGTCTT 
GAGGTGAGTC GCTGGGTAGA GGGCCGCGGA GTGACGTGGT CGGCCAAGTT GGAGGCTGAC 
CTGCCCCGAT CTTGGCAACG ACACGGTGAT CTCTTGTTGC TGAGTGAGGA CTGTTTCCAA 
GCCAAGCAAT GGAGACATCT GGAACCAGAA CTCTGGGAGA CGGTTGCCTC GGCACTTGGC 
GCCCAGCGTT TGGCCAAACG AGGGCGGGTG TCCCCGGATA GCACTCGGAC TCCAGCAGTG 
AGTCTGCTGC TGGGCGACCA CGGCTGGGTA GAGCATGTGG ATAATGGGAT TCGGTATAAG 
TTTGACGTGA CCCAGTGCAT GTTCTCCTTC GGAAACATCA CTGAGAAGCT TCGTGTGGCG 
TCGCTGCCCT GCGTCGGAGA AGTGCTGGTG GATCTCTATG CGGGAATTGG TTATTTTACC 
TTGCCTTTCC TGGTTCATGC CGAGGCTGCT TTCGTCCACG CTTGTGAGTG GAATCCCCAT 
GCGGTAGTTG CTCTGAGAAA TAACCTTGAA CTCAATGGAG TAGCTGATCG ATGCCAAATA 
CACTTTGGGG ATAACAGAAA ACTGAAGCTA TCAAACGTTG CCGACCGGGT GAATCTGGGG 
CTGATTCCCA GCTCTGAAGA AGGCTGGCCC ATTGCCTGCA GAGTGCTAAA ACAGGATGCT 
GGAGGCATTT TGCATATTCA CCAAAATGTG GAGTCTTTCC CAGGGAAGAC TCTCCAGCCT 
CCTGGAAGCA GTGAGATGGA GGAGCATTGG CCTTCTCCTC ATCAGATTAT CAGCAACCAG 
TTGAATAATG GAGCTACCAG TGATTCTAGG AGGAAAACGC TGTCAGTGGC CACCAAGCCA 
GAGTGGCAGA GGTGGGCCAA AGCTGCAGAG ACTCGAATTG CCACACTTCT TCATCAAGTG 
CATGGAAAAC GGTGGAAGAC ACAAATCTTG CACATCCAAC CAGTGAAATC CTATGCTCCC 
CACGTGGATC ACATAGTCTT GGATTTGGAA TGCCGCCCCT GTCATCTAGT TGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019723.1
CDS	43..1359
Translation	MEGAGGKPTAVVAVVTEPRFTQRYREYLEKHKLLDRQHRVKKLRDGTVALPVLREALLEQHLRELRNRVAPGSTCVPTQLLDPVPSKKAQSYSPAQRLCLEVSRWVEGRGVTWSAKLEADLPRSWQRHGDLLLLSEDCFQAKQWRHLEPELWETVASALGAQRLAKRGRVSPDSTRTPAVSLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLPCVGEVLVDLYAGIGYFTLPFLVHAEAAFVHACEWNPHAVVALRNNLELNGVADRCQIHFGDNRKLKLSNVADRVNLGLIPSSEEGWPIACRVLKQDAGGILHIHQNVESFPGKTLQPPGSSEMEEHWPSPHQIISNQLNNGATSDSRRKTLSVATKPEWQRWAKAAETRIATLLHQVHGKRWKTQILHIQPVKSYAPHVDHIVLDLECRPCHLVG

Target ORF information:

RefSeq Version	NM_001024552.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 12 homolog (TRMT12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024552.1

ORF Insert Sequence:

ATGGAAGGGG CAGGCGGGAA GCCCACGGCT GTTGTTGCAG TTGTGACTGA GCCTCGATTT 
ACTCAGCGAT ACAGGGAATA TCTCGAGAAG CACAAACTCC TGGATAGACA GCACCGTGTG 
AAAAAGCTGC GGGATGGCAC CGTGGCGCTA CCGGTTCTGA GAGAGGCCCT CCTTGAGCAG 
CATCTGCGAG AGCTGAGGAA TCGTGTGGCT CCAGGCAGCA CCTGTGTGCC GACGCAGCTC 
CTGGATCCTG TTCCTTCAAA GAAGGCCCAG AGTTACTCAC CTGCCCAAAG GCTGTGTCTT 
GAGGTGAGTC GCTGGGTAGA GGGCCGCGGA GTGACGTGGT CGGCCAAGTT GGAGGCTGAC 
CTGCCCCGAT CTTGGCAACG ACACGGTGAT CTCTTGTTGC TGAGTGAGGA CTGTTTCCAA 
GCCAAGCAAT GGAGACATCT GGAACCAGAA CTCTGGGAGA CGGTTGCCTC GGCACTTGGC 
GCCCAGCGTT TGGCCAAACG AGGGCGGGTG TCCCCGGATA GCACTCGGAC TCCAGCAGTG 
AGTCTGCTGC TGGGCGACCA CGGCTGGGTA GAGCATGTGG ATAATGGGAT TCGGTATAAG 
TTTGACGTGA CCCAGTGCAT GTTCTCCTTC GGAAACATCA CTGAGAAGCT TCGTGTGGCG 
TCGCTGCCCT GCGTCGGAGA AGTGCTGGTG GATCTCTATG CGGGAATTGG TTATTTTACC 
TTGCCTTTCC TGGTTCATGC CGAGGCTGCT TTCGTCCACG CTTGTGAGTG GAATCCCCAT 
GCGGTAGTTG CTCTGAGAAA TAACCTTGAA CTCAATGGAG TAGCTGATCG ATGCCAAATA 
CACTTTGGGG ATAACAGAAA ACTGAAGCTA TCAAACGTTG CCGACCGGGT GAATCTGGGG 
CTGATTCCCA GCTCTGAAGA AGGCTGGCCC ATTGCCTGCA GAGTGCTAAA ACAGGATGCT 
GGAGGCATTT TGCATATTCA CCAAAATGTG GAGTCTTTCC CAGGGAAGAC TCTCCAGCCT 
CCTGGAAGCA GTGAGATGGA GGAGCATTGG CCTTCTCCTC ATCAGATTAT CAGCAACCAG 
TTGAATAATG GAGCTACCAG TGATTCTAGG AGGAAAACGC TGTCAGTGGC CACCAAGCCA 
GAGTGGCAGA GGTGGGCCAA AGCTGCAGAG ACTCGAATTG CCACACTTCT TCATCAAGTG 
CATGGAAAAC GGTGGAAGAC ACAAATCTTG CACATCCAAC CAGTGAAATC CTATGCTCCC 
CACGTGGATC ACATAGTCTT GGATTTGGAA TGCCGCCCCT GTCATCTAGT TGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT12 ( XM_010811956.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810258.1 Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa25891
Clone ID Related Accession (Same CDS sequence)	XM_010811956.2 , XM_005215368.3 , NM_001024552.2 , XM_005215366.3 , XM_005215367.3
Accession Version	XM_010811956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	tRNA wybutosine-synthesizing protein 2 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104392.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810258.1
CDS	54..1442
Translation	MRGCAEAKQREFRGRSRRDCGASNMEGAGGKPTAVVAVVTEPRFTQRYREYLEKHKLLDRQHRVKKLRDGTVALPVLREALLEQHLRELRNRVAPGSTCVPTQLLDPVPSKKAQSYSPAQRLCLEVSRWVEGRGVTWSAELEADLPRSWQRHGDLLLLSEDCFQAKQWRHLEPELWETVASALGAQRLAKRGRVSPDSTRTPAVSLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLPCAGEVLVDLYAGIGYFTLPFLVHAEAAFVHACEWNPHAVVALRNNLELNGVADRCQIHFGDNRKLKLSNVADRVNLGLIPSSEEGWPVACRVLKQDAGGILHIHQNVESFPGKTLQPPGSSEMEEHWPSPHQIISNQLNNGATSDSRRKTLSVATKPEWQRWAKAAETRIATLLHQVHGKRWKTQILHIQPVKSYAPHVDHIVLDLECRPCHLVG

Target ORF information:

RefSeq Version	XM_010811956.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811956.2

ORF Insert Sequence:

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT12 ( XM_005215368.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215425.1 Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa25891
Clone ID Related Accession (Same CDS sequence)	XM_010811956.2 , XM_005215368.3 , NM_001024552.2 , XM_005215366.3 , XM_005215367.3
Accession Version	XM_005215368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	tRNA wybutosine-synthesizing protein 2 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104392.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215368.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215425.1
CDS	54..1442
Translation	MRGCAEAKQREFRGRSRRDCGASNMEGAGGKPTAVVAVVTEPRFTQRYREYLEKHKLLDRQHRVKKLRDGTVALPVLREALLEQHLRELRNRVAPGSTCVPTQLLDPVPSKKAQSYSPAQRLCLEVSRWVEGRGVTWSAELEADLPRSWQRHGDLLLLSEDCFQAKQWRHLEPELWETVASALGAQRLAKRGRVSPDSTRTPAVSLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLPCAGEVLVDLYAGIGYFTLPFLVHAEAAFVHACEWNPHAVVALRNNLELNGVADRCQIHFGDNRKLKLSNVADRVNLGLIPSSEEGWPVACRVLKQDAGGILHIHQNVESFPGKTLQPPGSSEMEEHWPSPHQIISNQLNNGATSDSRRKTLSVATKPEWQRWAKAAETRIATLLHQVHGKRWKTQILHIQPVKSYAPHVDHIVLDLECRPCHLVG

Target ORF information:

RefSeq Version	XM_005215368.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215368.3

ORF Insert Sequence:

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT12 ( XM_005215366.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215423.1 Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa25891
Clone ID Related Accession (Same CDS sequence)	XM_010811956.2 , XM_005215368.3 , NM_001024552.2 , XM_005215366.3 , XM_005215367.3
Accession Version	XM_005215366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	tRNA wybutosine-synthesizing protein 2 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104392.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215366.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215423.1
CDS	54..1442
Translation	MRGCAEAKQREFRGRSRRDCGASNMEGAGGKPTAVVAVVTEPRFTQRYREYLEKHKLLDRQHRVKKLRDGTVALPVLREALLEQHLRELRNRVAPGSTCVPTQLLDPVPSKKAQSYSPAQRLCLEVSRWVEGRGVTWSAELEADLPRSWQRHGDLLLLSEDCFQAKQWRHLEPELWETVASALGAQRLAKRGRVSPDSTRTPAVSLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLPCAGEVLVDLYAGIGYFTLPFLVHAEAAFVHACEWNPHAVVALRNNLELNGVADRCQIHFGDNRKLKLSNVADRVNLGLIPSSEEGWPVACRVLKQDAGGILHIHQNVESFPGKTLQPPGSSEMEEHWPSPHQIISNQLNNGATSDSRRKTLSVATKPEWQRWAKAAETRIATLLHQVHGKRWKTQILHIQPVKSYAPHVDHIVLDLECRPCHLVG

Target ORF information:

RefSeq Version	XM_005215366.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215366.3

ORF Insert Sequence:

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT12 ( XM_005215367.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215424.1 Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa25891
Clone ID Related Accession (Same CDS sequence)	XM_010811956.2 , XM_005215368.3 , NM_001024552.2 , XM_005215366.3 , XM_005215367.3
Accession Version	XM_005215367.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	tRNA wybutosine-synthesizing protein 2 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104392.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215367.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215424.1
CDS	54..1442
Translation	MRGCAEAKQREFRGRSRRDCGASNMEGAGGKPTAVVAVVTEPRFTQRYREYLEKHKLLDRQHRVKKLRDGTVALPVLREALLEQHLRELRNRVAPGSTCVPTQLLDPVPSKKAQSYSPAQRLCLEVSRWVEGRGVTWSAELEADLPRSWQRHGDLLLLSEDCFQAKQWRHLEPELWETVASALGAQRLAKRGRVSPDSTRTPAVSLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLPCAGEVLVDLYAGIGYFTLPFLVHAEAAFVHACEWNPHAVVALRNNLELNGVADRCQIHFGDNRKLKLSNVADRVNLGLIPSSEEGWPVACRVLKQDAGGILHIHQNVESFPGKTLQPPGSSEMEEHWPSPHQIISNQLNNGATSDSRRKTLSVATKPEWQRWAKAAETRIATLLHQVHGKRWKTQILHIQPVKSYAPHVDHIVLDLECRPCHLVG

Target ORF information:

RefSeq Version	XM_005215367.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215367.3

ORF Insert Sequence:

ATGCGTGGAT GTGCAGAGGC AAAGCAGAGG GAGTTTCGTG GAAGGAGTCG GAGAGACTGT 
GGGGCCAGCA ACATGGAAGG GGCAGGCGGG AAGCCCACGG CTGTTGTTGC AGTTGTGACT 
GAGCCTCGAT TTACTCAGCG ATACAGGGAA TATCTCGAGA AGCACAAACT CCTGGATAGA 
CAGCACCGTG TGAAAAAGCT GCGGGATGGC ACCGTGGCGC TACCGGTTCT GAGAGAGGCC 
CTCCTTGAGC AGCATCTACG AGAGCTGAGG AATCGTGTGG CTCCAGGCAG CACCTGTGTG 
CCGACGCAGC TCCTGGATCC TGTTCCTTCA AAGAAGGCCC AGAGTTACTC ACCTGCCCAA 
AGGCTGTGTC TTGAGGTGAG TCGCTGGGTA GAGGGCCGCG GAGTGACGTG GTCGGCCGAG 
TTGGAGGCTG ACCTGCCCCG ATCTTGGCAA CGACACGGTG ACCTCTTGTT GCTGAGTGAG 
GACTGTTTCC AAGCCAAGCA ATGGAGACAT CTGGAACCAG AACTCTGGGA GACGGTTGCC 
TCGGCACTTG GCGCCCAGCG TTTGGCCAAA CGAGGGCGGG TGTCCCCGGA TAGCACTCGG 
ACTCCAGCAG TGAGTCTGCT GCTGGGCGAC CACGGCTGGG TAGAGCATGT GGATAATGGG 
ATTCGGTATA AGTTTGACGT GACCCAGTGC ATGTTCTCCT TCGGAAACAT CACTGAGAAG 
CTTCGTGTGG CGTCGCTGCC CTGCGCCGGA GAAGTGCTGG TGGATCTCTA TGCGGGAATT 
GGTTATTTTA CCTTGCCTTT CCTGGTTCAT GCCGAGGCTG CTTTCGTCCA CGCTTGTGAG 
TGGAATCCCC ATGCGGTAGT TGCTCTGAGA AATAACCTTG AACTCAATGG AGTAGCTGAT 
CGATGCCAAA TACACTTTGG GGATAACAGA AAACTGAAGC TATCAAACGT TGCCGACCGG 
GTGAATCTGG GGCTGATTCC CAGCTCTGAA GAAGGCTGGC CCGTTGCCTG CAGAGTGCTA 
AAACAGGATG CTGGAGGCAT TTTGCATATT CACCAAAATG TGGAGTCTTT CCCAGGGAAG 
ACTCTCCAGC CTCCTGGAAG CAGTGAGATG GAGGAGCATT GGCCTTCTCC TCATCAGATT 
ATCAGCAACC AGTTGAATAA TGGAGCTACC AGTGATTCTA GGAGGAAAAC GCTGTCAGTG 
GCCACCAAGC CAGAGTGGCA GAGGTGGGCC AAAGCTGCAG AGACTCGAAT TGCCACACTT 
CTTCATCAGG TGCATGGAAA ACGGTGGAAG ACACAAATCT TGCACATCCA ACCAGTGAAA 
TCCTATGCTC CCCACGTGGA TCACATAGTC TTGGATTTGG AATGCCGCCC CTGTCATCTA 
GTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ESTs.
Biochimica et biophysica acta1679(1)10-7(2004 Jul)
Casey OM,Fitzpatrick R,McInerney JO,Morris DG,Powell R,Sreenan JM

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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TRMT12 cDNA ORF clone, Bos taurus(cattle)

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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