RET cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RET
Entrez Gene ID	515924
Full Name	ret proto-oncogene
General protein information	Preferred Names ret proto-oncogene Names proto-oncogene tyrosine-protein kinase receptor Ret
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024986653.1	XP_024842421.1	proto-oncogene tyrosine-protein kinase receptor Ret isoform X2
NM_001191483.1	NP_001178412.1	proto-oncogene tyrosine-protein kinase receptor Ret precursor
XM_024986652.1	XP_024842420.1	proto-oncogene tyrosine-protein kinase receptor Ret isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RET	NP_001184028.1
Bos taurus (cattle)	RET	NP_001178412.1
Homo sapiens (human)	RET	NP_066124.1
Macaca mulatta (Rhesus monkey)	RET	XP_002805672.1
Rattus norvegicus (Norway rat)	Ret	NP_036775.2
Danio rerio (zebrafish)	ret	NP_858048.2
Mus musculus (house mouse)	Ret	NP_033076.2
Xenopus tropicalis (tropical clawed frog)	ret	XP_002933941.2
Pan troglodytes (chimpanzee)	RET	XP_003312612.1
Gallus gallus (chicken)	RET	NP_990521.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RET gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RET cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa37777	NM_001191483.1 Latest version!	Bos taurus ret proto-oncogene (RET), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa231595	XM_024986652.1 Latest version!	Bos taurus ret proto-oncogene (RET), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa231596	XM_024986653.1 Latest version!	Bos taurus ret proto-oncogene (RET), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RET ( NM_001191483.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178412.1 Bos taurus ret proto-oncogene (RET), mRNA.

Price & Availability↑

CloneID	OBa37777
Clone ID Related Accession (Same CDS sequence)	NM_001191483.1
Accession Version	NM_001191483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548086400000
Organism	Bos taurus(cattle)
Product	proto-oncogene tyrosine-protein kinase receptor Ret precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC149315.1 and NKLS02000028.1. On Apr 19, 2011 this sequence version replaced XM_002703353.1. ##Evidence-Data-START## Transcript exon combination :: BC149315.1, SRR6764281.37325.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAAGG CGACGGCCAG CACCGTGGGG CTGCGGTTGC TGCTTCTGCT GCTGCCCCTG 
CTCGGCGAAG CCCCGCTGGG ACTCTACTTC TCCAGGGATG CTTACTGGGA GAAGCTATAC 
GTGGACCAGC CGGCCGGCAC GCCCCTGCTC TATGTCCACG CCCTGCGGGA CGTGCCGGAG 
GAGGTGCCCA GCTTCCGCCT GGGCCAGCAC CTCTACGGCG CCTACCGCAC GCGGCTGCAC 
GAGAACGACT GGGTCCGCAT CCAGGAGGAC ACGGGGCTTC TCTACCTTAA CCAGAGCCTG 
GACCACAGCT CCTGGGAGAA GCTCAACATC CGCAACGGCG GCTTCCCACT GCTTACCGTC 
TATCTCTGGG TCTTCCTGTC ACCCGCGTCC GTTCGTGAGG GCGAGTGCCA GTGGCCGGGC 
TGTGCCCGTG TGTACTTCTC CGTCATCAAT GCCTCCTTCC CAGCTTGCGG CGCCCTCAAA 
CCCCGGGAGC TCTGCTTCCC CGAGTCAGGC CTGTCCTTCC GCATCCGGGA GAACAGGCCC 
CCCGGCACCT TCCACCAGTT CCGCCTGCTG CCCGTGCAGT TCCTCTGCCC CAACGTCAGC 
GTGGCCTACA GGCTCCTGGG AGGTGAGAGT CTGCCTTTCC GCTGCGATCC GGACAGCCTG 
GAGGTGAGCA CGCGCTGGGC CCTGGACCGC GAGCAGCGGG AGAAGTATGA GCTGGTGGCC 
GCGTGCACGG TTCGCGTGGG CGCGCGCGAG GAGGAGGTGA TGGTGCCCTT CCCCGTGACC 
GTGTACGACG AGGACGACTC TGCGCCCACC TTCCTCGGGG GCGTCGACAG CGCCAGCGCC 
GTGGTGGAGT TCAAGCGGAA GGAGGGCACA GTGGTAGCCA CGTTACGTGT CTTCGATGCA 
GACGTGGTGC CAGCCTCCGG GGAGCTCCTG AGACGGTACA CGAACACGCT GCTCTCTGGG 
GACCCCAAGG CCCTCCGGAC CTTCCGAGTG GAGCACGCGC CCAATGAGAC CTTGGCCCAG 
GCCAACGGCA GCTTTGTGAG GGCAACCGTG CATGACTACA AGCTGGTTCT CAACCGGAGC 
CTCCCCATCT CGGAGAGCTG GGCTGTGCAG CTGGCCGTGC TGGTCAACGA CTCAGACTTC 
CAGGGCCCAG GGGAGGGTGT CGTCCTCCTC CACTTCAACG TGTCCGTGCT GCCCGTCAGC 
CTGCGCCTGC CCAGCGCCTA CTCCTTCTCC GTGAGCAGGC GGGCCCACCG CTTCGCCCAG 
ATCGGGAAAG TCTGCGTGGA AAACTGCCAG GAGTTCAGCG GCATCCAAGT GCAGTACAGG 
CTGCAGCCCT CCAGCGCCAA CTGCAGCGCC CTGGGGATGG TCACCTCGGC TGAGGACACC 
TCGGGGACCC TGTTCGTGAA TGACATAGAG GCCCTGCAGC GGCCCGAGTG TGCCCAGCTC 
CAGTACACGG TGGTGGCCGC CGAGAAACCA ACCCGCCAGC AAGCCCAGGC CCCGCTGCTC 
GTTAGCGTGG AAGGGATATA CGTGCCTGAG GAGCCTGGCT GCCCCCTGTC CTGCGCGGTC 
AGCAAGAGGC GGCCCGAATG TGAGGAATGC GGCGGCCTGG GCTCTCTAAC GGGCAGGTGC 
GAGTGGAGAC AGGGAGACGG CAAAGGGATC ACCAGGAACT TCTCCACCTG CTCCCCCAGC 
ATCAGGACCT GCCCCGATGG CCACTGTGAC GCCGTGGAGA GCAGAGACCC CAACATCTGC 
CCCCAGGACT GCCTCCGGGG CGGCAGCATC ATTGGTGGGC ACGAGCCTGG GGAGCGCCGG 
GGGATTAAAG CTGGCTTCGG TATCTGCAAC TGCTTCCCTG AGGAGAAGAA GTTCTTCTGC 
GAGCCAGAGG ACAGCCAGGA CTCCCTGTGT GATGAGCTCT GCCGCACAGT GATCACGGCG 
GCCGTGCTCT TCTCCTTCAT CGTGTCTGTG CTGCTGTCCA CGTTCTGCAT CCACCGCTAC 
CACAAGAACG CCCACAAGCC GCCCATTGCC TCGGCCGAGA TGACCTTCCG CCGGCCCGCC 
CAAGCCTTCC CGGTCAGCTA CTCCTCGTCT GGCGCCCGCC GGCCCTCTCT GGACTCCATG 
GAGAACCAGG TCTCGGTGGA CACCTTCAAG ATCCCGGAGG ATCCCAAGTG GGAATTCCCT 
CGGAAGAACT TGGTGCTTGG AAAAACTCTG GGAGAAGGCG AATTTGGAAA AGTGGTCAAG 
GCCACAGCCT TCCGGCTGAA AGGCAAAGCG GGGTACACGA CTGTGGCTGT GAAGATGCTG 
AAAGAGAATG CCTCCCCAAG CGAGCTGCGG GACCTGCTAT CAGAGTTCAA CCTCCTGAAG 
CAGGTCAACC ACCCGCAAGT CATCAAGCTG TACGGGGCCT GCAGCCAGGA CGGGCCACTG 
CTCCTCATCG TGGAGTATGC CAAGTACGGC TCCCTGCGGG GCTTCCTCCG AGAGAGCCGC 
AAGGCGGGGC CTGGCTACGT GGGCAGCGGG GGCAGCCGCA ATTCCAGTTA CCTGGACAAC 
CCGGAGGAGC GGGCCCTGAC CATGGGCGAC CTCATCTCCT TTGCCTGGCA GATCTCACGA 
GGGATGCAGT ACCTGGCTGA GATGAAGCTT GTCCATCGGG ACTTGGCAGC CAGAAACGTC 
CTGGTAGCTG AGGGCCGCAG GATGAAGATC TCAGATTTTG GTCTGTCCCG AGATGTTTAT 
GAAGAGGATT CCTATGTGAA GAGGAGCAAG GGTCGGATCC CAGTCAAGTG GATGGCCATT 
GAGTCCCTCT TCGATCATAT CTATACCACC CAGAGTGATG TGTGGTCCTT TGGCGTTCTG 
CTGTGGGAGA TTGTGACCCT GGGCGGCAAC CCCTACCCTG GGATTCCTCC AGAGCGGCTC 
TTCAACCTTC TGAAGACAGG CTACCGGATG GAGAGGCCGG ACAACTGCAG TGAGGAGATG 
TACAATCTGA TGCTGCAGTG CTGGAAGCAG GAACCAGACA AGAGGCCAGT GTTTGCCGAC 
ATCGGCAAAG ACCTGGATAA GATGATGGTT AAGAGCAGAG ACTACCTGGA CCTGGCCGCG 
TCCACCCCAT CTGACTCCCT GCTGTATGAC GACGGCCTGT CGGAGGAGGA GACGCCCCTG 
GTGGACTGTA ATAACGCTCC CCTCCCTCGC ACCCTCCCCT CCACGTGGAT TGAAAACAAA 
CTCTATGGTA GAATTTCACA TGCATTTACT AGATTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178412.1
CDS	200..3418
Translation	MAKATASTVGLRLLLLLLPLLGEAPLGLYFSRDAYWEKLYVDQPAGTPLLYVHALRDVPEEVPSFRLGQHLYGAYRTRLHENDWVRIQEDTGLLYLNQSLDHSSWEKLNIRNGGFPLLTVYLWVFLSPASVREGECQWPGCARVYFSVINASFPACGALKPRELCFPESGLSFRIRENRPPGTFHQFRLLPVQFLCPNVSVAYRLLGGESLPFRCDPDSLEVSTRWALDREQREKYELVAACTVRVGAREEEVMVPFPVTVYDEDDSAPTFLGGVDSASAVVEFKRKEGTVVATLRVFDADVVPASGELLRRYTNTLLSGDPKALRTFRVEHAPNETLAQANGSFVRATVHDYKLVLNRSLPISESWAVQLAVLVNDSDFQGPGEGVVLLHFNVSVLPVSLRLPSAYSFSVSRRAHRFAQIGKVCVENCQEFSGIQVQYRLQPSSANCSALGMVTSAEDTSGTLFVNDIEALQRPECAQLQYTVVAAEKPTRQQAQAPLLVSVEGIYVPEEPGCPLSCAVSKRRPECEECGGLGSLTGRCEWRQGDGKGITRNFSTCSPSIRTCPDGHCDAVESRDPNICPQDCLRGGSIIGGHEPGERRGIKAGFGICNCFPEEKKFFCEPEDSQDSLCDELCRTVITAAVLFSFIVSVLLSTFCIHRYHKNAHKPPIASAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDTFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVNHPQVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKAGPGYVGSGGSRNSSYLDNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRDVYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADIGKDLDKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNNAPLPRTLPSTWIENKLYGRISHAFTRF

Target ORF information:

RefSeq Version	NM_001191483.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ret proto-oncogene (RET), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191483.1

ORF Insert Sequence:

ATGGCGAAGG CGACGGCCAG CACCGTGGGG CTGCGGTTGC TGCTTCTGCT GCTGCCCCTG 
CTCGGCGAAG CCCCGCTGGG ACTCTACTTC TCCAGGGATG CTTACTGGGA GAAGCTATAC 
GTGGACCAGC CGGCCGGCAC GCCCCTGCTC TATGTCCACG CCCTGCGGGA CGTGCCGGAG 
GAGGTGCCCA GCTTCCGCCT GGGCCAGCAC CTCTACGGCG CCTACCGCAC GCGGCTGCAC 
GAGAACGACT GGGTCCGCAT CCAGGAGGAC ACGGGGCTTC TCTACCTTAA CCAGAGCCTG 
GACCACAGCT CCTGGGAGAA GCTCAACATC CGCAACGGCG GCTTCCCACT GCTTACCGTC 
TATCTCTGGG TCTTCCTGTC ACCCGCGTCC GTTCGTGAGG GCGAGTGCCA GTGGCCGGGC 
TGTGCCCGTG TGTACTTCTC CGTCATCAAT GCCTCCTTCC CAGCTTGCGG CGCCCTCAAA 
CCCCGGGAGC TCTGCTTCCC CGAGTCAGGC CTGTCCTTCC GCATCCGGGA GAACAGGCCC 
CCCGGCACCT TCCACCAGTT CCGCCTGCTG CCCGTGCAGT TCCTCTGCCC CAACGTCAGC 
GTGGCCTACA GGCTCCTGGG AGGTGAGAGT CTGCCTTTCC GCTGCGATCC GGACAGCCTG 
GAGGTGAGCA CGCGCTGGGC CCTGGACCGC GAGCAGCGGG AGAAGTATGA GCTGGTGGCC 
GCGTGCACGG TTCGCGTGGG CGCGCGCGAG GAGGAGGTGA TGGTGCCCTT CCCCGTGACC 
GTGTACGACG AGGACGACTC TGCGCCCACC TTCCTCGGGG GCGTCGACAG CGCCAGCGCC 
GTGGTGGAGT TCAAGCGGAA GGAGGGCACA GTGGTAGCCA CGTTACGTGT CTTCGATGCA 
GACGTGGTGC CAGCCTCCGG GGAGCTCCTG AGACGGTACA CGAACACGCT GCTCTCTGGG 
GACCCCAAGG CCCTCCGGAC CTTCCGAGTG GAGCACGCGC CCAATGAGAC CTTGGCCCAG 
GCCAACGGCA GCTTTGTGAG GGCAACCGTG CATGACTACA AGCTGGTTCT CAACCGGAGC 
CTCCCCATCT CGGAGAGCTG GGCTGTGCAG CTGGCCGTGC TGGTCAACGA CTCAGACTTC 
CAGGGCCCAG GGGAGGGTGT CGTCCTCCTC CACTTCAACG TGTCCGTGCT GCCCGTCAGC 
CTGCGCCTGC CCAGCGCCTA CTCCTTCTCC GTGAGCAGGC GGGCCCACCG CTTCGCCCAG 
ATCGGGAAAG TCTGCGTGGA AAACTGCCAG GAGTTCAGCG GCATCCAAGT GCAGTACAGG 
CTGCAGCCCT CCAGCGCCAA CTGCAGCGCC CTGGGGATGG TCACCTCGGC TGAGGACACC 
TCGGGGACCC TGTTCGTGAA TGACATAGAG GCCCTGCAGC GGCCCGAGTG TGCCCAGCTC 
CAGTACACGG TGGTGGCCGC CGAGAAACCA ACCCGCCAGC AAGCCCAGGC CCCGCTGCTC 
GTTAGCGTGG AAGGGATATA CGTGCCTGAG GAGCCTGGCT GCCCCCTGTC CTGCGCGGTC 
AGCAAGAGGC GGCCCGAATG TGAGGAATGC GGCGGCCTGG GCTCTCTAAC GGGCAGGTGC 
GAGTGGAGAC AGGGAGACGG CAAAGGGATC ACCAGGAACT TCTCCACCTG CTCCCCCAGC 
ATCAGGACCT GCCCCGATGG CCACTGTGAC GCCGTGGAGA GCAGAGACCC CAACATCTGC 
CCCCAGGACT GCCTCCGGGG CGGCAGCATC ATTGGTGGGC ACGAGCCTGG GGAGCGCCGG 
GGGATTAAAG CTGGCTTCGG TATCTGCAAC TGCTTCCCTG AGGAGAAGAA GTTCTTCTGC 
GAGCCAGAGG ACAGCCAGGA CTCCCTGTGT GATGAGCTCT GCCGCACAGT GATCACGGCG 
GCCGTGCTCT TCTCCTTCAT CGTGTCTGTG CTGCTGTCCA CGTTCTGCAT CCACCGCTAC 
CACAAGAACG CCCACAAGCC GCCCATTGCC TCGGCCGAGA TGACCTTCCG CCGGCCCGCC 
CAAGCCTTCC CGGTCAGCTA CTCCTCGTCT GGCGCCCGCC GGCCCTCTCT GGACTCCATG 
GAGAACCAGG TCTCGGTGGA CACCTTCAAG ATCCCGGAGG ATCCCAAGTG GGAATTCCCT 
CGGAAGAACT TGGTGCTTGG AAAAACTCTG GGAGAAGGCG AATTTGGAAA AGTGGTCAAG 
GCCACAGCCT TCCGGCTGAA AGGCAAAGCG GGGTACACGA CTGTGGCTGT GAAGATGCTG 
AAAGAGAATG CCTCCCCAAG CGAGCTGCGG GACCTGCTAT CAGAGTTCAA CCTCCTGAAG 
CAGGTCAACC ACCCGCAAGT CATCAAGCTG TACGGGGCCT GCAGCCAGGA CGGGCCACTG 
CTCCTCATCG TGGAGTATGC CAAGTACGGC TCCCTGCGGG GCTTCCTCCG AGAGAGCCGC 
AAGGCGGGGC CTGGCTACGT GGGCAGCGGG GGCAGCCGCA ATTCCAGTTA CCTGGACAAC 
CCGGAGGAGC GGGCCCTGAC CATGGGCGAC CTCATCTCCT TTGCCTGGCA GATCTCACGA 
GGGATGCAGT ACCTGGCTGA GATGAAGCTT GTCCATCGGG ACTTGGCAGC CAGAAACGTC 
CTGGTAGCTG AGGGCCGCAG GATGAAGATC TCAGATTTTG GTCTGTCCCG AGATGTTTAT 
GAAGAGGATT CCTATGTGAA GAGGAGCAAG GGTCGGATCC CAGTCAAGTG GATGGCCATT 
GAGTCCCTCT TCGATCATAT CTATACCACC CAGAGTGATG TGTGGTCCTT TGGCGTTCTG 
CTGTGGGAGA TTGTGACCCT GGGCGGCAAC CCCTACCCTG GGATTCCTCC AGAGCGGCTC 
TTCAACCTTC TGAAGACAGG CTACCGGATG GAGAGGCCGG ACAACTGCAG TGAGGAGATG 
TACAATCTGA TGCTGCAGTG CTGGAAGCAG GAACCAGACA AGAGGCCAGT GTTTGCCGAC 
ATCGGCAAAG ACCTGGATAA GATGATGGTT AAGAGCAGAG ACTACCTGGA CCTGGCCGCG 
TCCACCCCAT CTGACTCCCT GCTGTATGAC GACGGCCTGT CGGAGGAGGA GACGCCCCTG 
GTGGACTGTA ATAACGCTCC CCTCCCTCGC ACCCTCCCCT CCACGTGGAT TGAAAACAAA 
CTCTATGGTA GAATTTCACA TGCATTTACT AGATTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RET ( XM_024986652.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842420.1 Bos taurus ret proto-oncogene (RET), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231595
Clone ID Related Accession (Same CDS sequence)	XM_024986652.1
Accession Version	XM_024986652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	proto-oncogene tyrosine-protein kinase receptor Ret isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGG CGACGGCCAG CACCGTGGGG CTGCGGTTGC TGCTTCTGCT GCTGCCCCTG 
CTCGGCGAAG CCCCGCTGGG ACTCTACTTC TCCAGGGATG CTTACTGGGA GAAGCTATAC 
GTGGACCAGC CGGCCGGCAC GCCCCTGCTC TATGTCCACG CCCTGCGGGA CGTGCCGGAG 
GAGGTGCCCA GCTTCCGCCT GGGCCAGCAC CTCTACGGCG CCTACCGCAC GCGGCTGCAC 
GAGAACGACT GGGTCCGCAT CCAGGAGGAC ACGGGGCTTC TCTACCTTAA CCAGAGCCTG 
GACCACAGCT CCTGGGAGAA GCTCAACATC CGCAACGGCG GCTTCCCACT GCTTACCGTC 
TATCTCTGGG TCTTCCTGTC ACCCGCGTCC GTTCGTGAGG GCGAGTGCCA GTGGCCGGGC 
TGTGCCCGTG TGTACTTCTC CGTCATCAAT GCCTCCTTCC CAGCTTGCGG CGCCCTCAAA 
CCCCGGGAGC TCTGCTTCCC CGAGTCAGGC CTGTCCTTCC GCATCCGGGA GAACAGGCCC 
CCCGGCACCT TCCACCAGTT CCGCCTGCTG CCCGTGCAGT TCCTCTGCCC CAACGTCAGC 
GTGGCCTACA GGCTCCTGGG AGGTGAGAGT CTGCCTTTCC GCTGCGATCC GGACAGCCTG 
GAGGTGAGCA CGCGCTGGGC CCTGGACCGC GAGCAGCGGG AGAAGTATGA GCTGGTGGCC 
GCGTGCACGG TTCGCGTGGG CGCGCGCGAG GAGGAGGTGA TGGTGCCCTT CCCCGTGACC 
GTGTACGACG AGGACGACTC TGCGCCCACC TTCCTCGGGG GCGTCGACAG CGCCAGCGCC 
GTGGTGGAGT TCAAGCGGAA GGAGGGCACA GTGGTAGCCA CGTTACGTGT CTTCGATGCA 
GACGTGGTGC CAGCCTCCGG GGAGCTCCTG AGACGGTACA CGAACACGCT GCTCTCTGGG 
GACCCCAAGG CCCTCCGGAC CTTCCGAGTG GAGCACGCGC CCAATGAGAC CTTGGCCCAG 
GCCAACGGCA GCTTTGTGAG GGCAACCGTG CATGACTACA AGCTGGTTCT CAACCGGAGC 
CTCCCCATCT CGGAGAGCTG GGCTGTGCAG CTGGCCGTGC TGGTCAACGA CTCAGACTTC 
CAGGGCCCAG GGGAGGGTGT CGTCCTCCTC CACTTCAACG TGTCCGTGCT GCCCGTCAGC 
CTGCGCCTGC CCAGCGCCTA CTCCTTCTCC GTGAGCAGGC GGGCCCACCG CTTCGCCCAG 
ATCGGGAAAG TCTGCGTGGA AAACTGCCAG GAGTTCAGCG GCATCCAAGT GCAGTACAGG 
CTGCAGCCCT CCAGCGCCAA CTGCAGCGCC CTGGGGATGG TCACCTCGGC TGAGGACACC 
TCGGGGACCC TGTTCGTGAA TGACATAGAG GCCCTGCAGC GGCCCGAGTG TGCCCAGCTC 
CAGTACACGG TGGTGGCCGC CGAGAAACCA ACCCGCCAGC AAGCCCAGGC CCCGCTGCTC 
GTTAGCGTGG AAGGGATATA CGTGCCTGAG GAGCCTGGCT GCCCCCTGTC CTGCGCGGTC 
AGCAAGAGGC GGCCCGAATG TGAGGAATGC GGCGGCCTGG GCTCTCTAAC GGGCAGGTGC 
GAGTGGAGAC AGGGAGACGG CAAAGGGATC ACCAGGAACT TCTCCACCTG CTCCCCCAGC 
ATCAGGACCT GCCCCGATGG CCACTGTGAC GCCGTGGAGA GCAGAGACCC CAACATCTGC 
CCCCAGGACT GCCTCCGGGG CGGCAGCATC ATTGGTGGGC ACGAGCCTGG GGAGCGCCGG 
GGGATTAAAG CTGGCTTCGG TATCTGCAAC TGCTTCCCTG AGGAGAAGAA GTGCTTCTGC 
GAGCCAGAGG ACAGCCAGGA CTCCCTGTGT GATGAGCTCT GCCGCACAGT GATCACGGCG 
GCCGTGCTCT TCTCCTTCAT CGTGTCTGTG CTGCTGTCCA CGTTCTGCAT CCACCGCTAC 
CACAAGAACG CCCACAAGCC GCCCATTGCC TCGGCCGAGA TGACCTTCCG CCGGCCCGCC 
CAAGCCTTCC CGGTCAGCTA CTCCTCGTCT GGCGCCCGCC GGCCCTCTCT GGACTCCATG 
GAGAACCAGG TCTCGGTGGA CACCTTCAAG ATCCCGGAGG ATCCCAAGTG GGAATTCCCT 
CGGAAGAACT TGGTGCTTGG AAAAACTCTG GGAGAAGGCG AATTTGGAAA AGTGGTCAAG 
GCCACAGCCT TCCGGCTGAA AGGCAAAGCG GGGTACACGA CTGTGGCTGT GAAGATGCTG 
AAAGAGAATG CCTCCCCAAG CGAGCTGCGG GACCTGCTAT CAGAGTTCAA CCTCCTGAAG 
CAGGTCAACC ACCCGCAAGT CATCAAGCTG TACGGGGCCT GCAGCCAGGA CGGGCCACTG 
CTCCTCATCG TGGAGTATGC CAAGTACGGC TCCCTGCGGG GCTTCCTCCG AGAGAGCCGC 
AAGGCGGGGC CTGGCTACGT GGGCAGCGGG GGCAGCCGCA ATTCCAGTTA CCTGGACAAC 
CCGGAGGAGC GGGCCCTGAC CATGGGCGAC CTCATCTCCT TTGCCTGGCA GATCTCACGA 
GGGATGCAGT ACCTGGCTGA GATGAAGCTT GTCCATCGGG ACTTGGCAGC CAGAAACGTC 
CTGGTAGCTG AGGGCCGCAG GATGAAGATC TCAGATTTTG GTCTGTCCCG AGATGTTTAT 
GAAGAGGATT CCTATGTGAA GAGGAGCAAG GGTCGGATCC CAGTCAAGTG GATGGCCATT 
GAGTCCCTCT TCGATCATAT CTATACCACC CAGAGTGATG TGTGGTCCTT TGGCGTTCTG 
CTGTGGGAGA TTGTGACCCT GGGCGGCAAC CCCTACCCTG GGATTCCTCC AGAGCGGCTC 
TTCAACCTTC TGAAGACAGG CTACCGGATG GAGAGGCCGG ACAACTGCAG TGAGGAGATG 
TACAATCTGA TGCTGCAGTG CTGGAAGCAG GAACCAGACA AGAGGCCAGT GTTTGCCGAC 
ATCAGCAAAG ACCTGGATAA GATGATGGTT AAGAGCAGAG ACTACCTGGA CCTGGCCGCG 
TCCACCCCAT CTGACTCCCT GCTGTATGAC GACGGCCTGT CGGAGGAGGA GACGCCCCTG 
GTGGACTGTA ATAACGCTCC CCTCCCTCGC ACCCTCCCCT CCACGTGGAT TGAAAACAAA 
CTCTATGGCA TGTCAGACCC GAACTGGCCT GAAGAGAGTC CTGTACCACT CACGAGAGCC 
GCTGGCACCA ACATTGGGTG TCCAAGATAT GCAAATGATA GTGTATATGC TAACTGGATG 
GTTTCACCCT CAGCGGCACA ATTAATGGAC GCGTTTGATA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842420.1
CDS	216..3560
Translation	MAKATASTVGLRLLLLLLPLLGEAPLGLYFSRDAYWEKLYVDQPAGTPLLYVHALRDVPEEVPSFRLGQHLYGAYRTRLHENDWVRIQEDTGLLYLNQSLDHSSWEKLNIRNGGFPLLTVYLWVFLSPASVREGECQWPGCARVYFSVINASFPACGALKPRELCFPESGLSFRIRENRPPGTFHQFRLLPVQFLCPNVSVAYRLLGGESLPFRCDPDSLEVSTRWALDREQREKYELVAACTVRVGAREEEVMVPFPVTVYDEDDSAPTFLGGVDSASAVVEFKRKEGTVVATLRVFDADVVPASGELLRRYTNTLLSGDPKALRTFRVEHAPNETLAQANGSFVRATVHDYKLVLNRSLPISESWAVQLAVLVNDSDFQGPGEGVVLLHFNVSVLPVSLRLPSAYSFSVSRRAHRFAQIGKVCVENCQEFSGIQVQYRLQPSSANCSALGMVTSAEDTSGTLFVNDIEALQRPECAQLQYTVVAAEKPTRQQAQAPLLVSVEGIYVPEEPGCPLSCAVSKRRPECEECGGLGSLTGRCEWRQGDGKGITRNFSTCSPSIRTCPDGHCDAVESRDPNICPQDCLRGGSIIGGHEPGERRGIKAGFGICNCFPEEKKCFCEPEDSQDSLCDELCRTVITAAVLFSFIVSVLLSTFCIHRYHKNAHKPPIASAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDTFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVNHPQVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKAGPGYVGSGGSRNSSYLDNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRDVYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNNAPLPRTLPSTWIENKLYGMSDPNWPEESPVPLTRAAGTNIGCPRYANDSVYANWMVSPSAAQLMDAFDS

Target ORF information:

RefSeq Version	XM_024986652.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ret proto-oncogene (RET), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986652.1

ORF Insert Sequence:

ATGGCGAAGG CGACGGCCAG CACCGTGGGG CTGCGGTTGC TGCTTCTGCT GCTGCCCCTG 
CTCGGCGAAG CCCCGCTGGG ACTCTACTTC TCCAGGGATG CTTACTGGGA GAAGCTATAC 
GTGGACCAGC CGGCCGGCAC GCCCCTGCTC TATGTCCACG CCCTGCGGGA CGTGCCGGAG 
GAGGTGCCCA GCTTCCGCCT GGGCCAGCAC CTCTACGGCG CCTACCGCAC GCGGCTGCAC 
GAGAACGACT GGGTCCGCAT CCAGGAGGAC ACGGGGCTTC TCTACCTTAA CCAGAGCCTG 
GACCACAGCT CCTGGGAGAA GCTCAACATC CGCAACGGCG GCTTCCCACT GCTTACCGTC 
TATCTCTGGG TCTTCCTGTC ACCCGCGTCC GTTCGTGAGG GCGAGTGCCA GTGGCCGGGC 
TGTGCCCGTG TGTACTTCTC CGTCATCAAT GCCTCCTTCC CAGCTTGCGG CGCCCTCAAA 
CCCCGGGAGC TCTGCTTCCC CGAGTCAGGC CTGTCCTTCC GCATCCGGGA GAACAGGCCC 
CCCGGCACCT TCCACCAGTT CCGCCTGCTG CCCGTGCAGT TCCTCTGCCC CAACGTCAGC 
GTGGCCTACA GGCTCCTGGG AGGTGAGAGT CTGCCTTTCC GCTGCGATCC GGACAGCCTG 
GAGGTGAGCA CGCGCTGGGC CCTGGACCGC GAGCAGCGGG AGAAGTATGA GCTGGTGGCC 
GCGTGCACGG TTCGCGTGGG CGCGCGCGAG GAGGAGGTGA TGGTGCCCTT CCCCGTGACC 
GTGTACGACG AGGACGACTC TGCGCCCACC TTCCTCGGGG GCGTCGACAG CGCCAGCGCC 
GTGGTGGAGT TCAAGCGGAA GGAGGGCACA GTGGTAGCCA CGTTACGTGT CTTCGATGCA 
GACGTGGTGC CAGCCTCCGG GGAGCTCCTG AGACGGTACA CGAACACGCT GCTCTCTGGG 
GACCCCAAGG CCCTCCGGAC CTTCCGAGTG GAGCACGCGC CCAATGAGAC CTTGGCCCAG 
GCCAACGGCA GCTTTGTGAG GGCAACCGTG CATGACTACA AGCTGGTTCT CAACCGGAGC 
CTCCCCATCT CGGAGAGCTG GGCTGTGCAG CTGGCCGTGC TGGTCAACGA CTCAGACTTC 
CAGGGCCCAG GGGAGGGTGT CGTCCTCCTC CACTTCAACG TGTCCGTGCT GCCCGTCAGC 
CTGCGCCTGC CCAGCGCCTA CTCCTTCTCC GTGAGCAGGC GGGCCCACCG CTTCGCCCAG 
ATCGGGAAAG TCTGCGTGGA AAACTGCCAG GAGTTCAGCG GCATCCAAGT GCAGTACAGG 
CTGCAGCCCT CCAGCGCCAA CTGCAGCGCC CTGGGGATGG TCACCTCGGC TGAGGACACC 
TCGGGGACCC TGTTCGTGAA TGACATAGAG GCCCTGCAGC GGCCCGAGTG TGCCCAGCTC 
CAGTACACGG TGGTGGCCGC CGAGAAACCA ACCCGCCAGC AAGCCCAGGC CCCGCTGCTC 
GTTAGCGTGG AAGGGATATA CGTGCCTGAG GAGCCTGGCT GCCCCCTGTC CTGCGCGGTC 
AGCAAGAGGC GGCCCGAATG TGAGGAATGC GGCGGCCTGG GCTCTCTAAC GGGCAGGTGC 
GAGTGGAGAC AGGGAGACGG CAAAGGGATC ACCAGGAACT TCTCCACCTG CTCCCCCAGC 
ATCAGGACCT GCCCCGATGG CCACTGTGAC GCCGTGGAGA GCAGAGACCC CAACATCTGC 
CCCCAGGACT GCCTCCGGGG CGGCAGCATC ATTGGTGGGC ACGAGCCTGG GGAGCGCCGG 
GGGATTAAAG CTGGCTTCGG TATCTGCAAC TGCTTCCCTG AGGAGAAGAA GTGCTTCTGC 
GAGCCAGAGG ACAGCCAGGA CTCCCTGTGT GATGAGCTCT GCCGCACAGT GATCACGGCG 
GCCGTGCTCT TCTCCTTCAT CGTGTCTGTG CTGCTGTCCA CGTTCTGCAT CCACCGCTAC 
CACAAGAACG CCCACAAGCC GCCCATTGCC TCGGCCGAGA TGACCTTCCG CCGGCCCGCC 
CAAGCCTTCC CGGTCAGCTA CTCCTCGTCT GGCGCCCGCC GGCCCTCTCT GGACTCCATG 
GAGAACCAGG TCTCGGTGGA CACCTTCAAG ATCCCGGAGG ATCCCAAGTG GGAATTCCCT 
CGGAAGAACT TGGTGCTTGG AAAAACTCTG GGAGAAGGCG AATTTGGAAA AGTGGTCAAG 
GCCACAGCCT TCCGGCTGAA AGGCAAAGCG GGGTACACGA CTGTGGCTGT GAAGATGCTG 
AAAGAGAATG CCTCCCCAAG CGAGCTGCGG GACCTGCTAT CAGAGTTCAA CCTCCTGAAG 
CAGGTCAACC ACCCGCAAGT CATCAAGCTG TACGGGGCCT GCAGCCAGGA CGGGCCACTG 
CTCCTCATCG TGGAGTATGC CAAGTACGGC TCCCTGCGGG GCTTCCTCCG AGAGAGCCGC 
AAGGCGGGGC CTGGCTACGT GGGCAGCGGG GGCAGCCGCA ATTCCAGTTA CCTGGACAAC 
CCGGAGGAGC GGGCCCTGAC CATGGGCGAC CTCATCTCCT TTGCCTGGCA GATCTCACGA 
GGGATGCAGT ACCTGGCTGA GATGAAGCTT GTCCATCGGG ACTTGGCAGC CAGAAACGTC 
CTGGTAGCTG AGGGCCGCAG GATGAAGATC TCAGATTTTG GTCTGTCCCG AGATGTTTAT 
GAAGAGGATT CCTATGTGAA GAGGAGCAAG GGTCGGATCC CAGTCAAGTG GATGGCCATT 
GAGTCCCTCT TCGATCATAT CTATACCACC CAGAGTGATG TGTGGTCCTT TGGCGTTCTG 
CTGTGGGAGA TTGTGACCCT GGGCGGCAAC CCCTACCCTG GGATTCCTCC AGAGCGGCTC 
TTCAACCTTC TGAAGACAGG CTACCGGATG GAGAGGCCGG ACAACTGCAG TGAGGAGATG 
TACAATCTGA TGCTGCAGTG CTGGAAGCAG GAACCAGACA AGAGGCCAGT GTTTGCCGAC 
ATCAGCAAAG ACCTGGATAA GATGATGGTT AAGAGCAGAG ACTACCTGGA CCTGGCCGCG 
TCCACCCCAT CTGACTCCCT GCTGTATGAC GACGGCCTGT CGGAGGAGGA GACGCCCCTG 
GTGGACTGTA ATAACGCTCC CCTCCCTCGC ACCCTCCCCT CCACGTGGAT TGAAAACAAA 
CTCTATGGCA TGTCAGACCC GAACTGGCCT GAAGAGAGTC CTGTACCACT CACGAGAGCC 
GCTGGCACCA ACATTGGGTG TCCAAGATAT GCAAATGATA GTGTATATGC TAACTGGATG 
GTTTCACCCT CAGCGGCACA ATTAATGGAC GCGTTTGATA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RET ( XM_024986653.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842421.1 Bos taurus ret proto-oncogene (RET), transcript variant X2, mRNA.

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CloneID	OBa231596
Clone ID Related Accession (Same CDS sequence)	XM_024986653.1
Accession Version	XM_024986653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	proto-oncogene tyrosine-protein kinase receptor Ret isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGG CGACGGCCAG CACCGTGGGG CTGCGGTTGC TGCTTCTGCT GCTGCCCCTG 
CTCGGCGAAG CCCCGCTGGG ACTCTACTTC TCCAGGGATG CTTACTGGGA GAAGCTATAC 
GTGGACCAGC CGGCCGGCAC GCCCCTGCTC TATGTCCACG CCCTGCGGGA CGTGCCGGAG 
GAGGTGCCCA GCTTCCGCCT GGGCCAGCAC CTCTACGGCG CCTACCGCAC GCGGCTGCAC 
GAGAACGACT GGGTCCGCAT CCAGGAGGAC ACGGGGCTTC TCTACCTTAA CCAGAGCCTG 
GACCACAGCT CCTGGGAGAA GCTCAACATC CGCAACGGCG GCTTCCCACT GCTTACCGTC 
TATCTCTGGG TCTTCCTGTC ACCCGCGTCC GTTCGTGAGG GCGAGTGCCA GTGGCCGGGC 
TGTGCCCGTG TGTACTTCTC CGTCATCAAT GCCTCCTTCC CAGCTTGCGG CGCCCTCAAA 
CCCCGGGAGC TCTGCTTCCC CGAGTCAGGC CTGTCCTTCC GCATCCGGGA GAACAGGCCC 
CCCGGCACCT TCCACCAGTT CCGCCTGCTG CCCGTGCAGT TCCTCTGCCC CAACGTCAGC 
GTGGCCTACA GGCTCCTGGG AGGTGAGAGT CTGCCTTTCC GCTGCGATCC GGACAGCCTG 
GAGGTGAGCA CGCGCTGGGC CCTGGACCGC GAGCAGCGGG AGAAGTATGA GCTGGTGGCC 
GCGTGCACGG TTCGCGTGGG CGCGCGCGAG GAGGAGGTGA TGGTGCCCTT CCCCGTGACC 
GTGTACGACG AGGACGACTC TGCGCCCACC TTCCTCGGGG GCGTCGACAG CGCCAGCGCC 
GTGGTGGAGT TCAAGCGGAA GGAGGGCACA GTGGTAGCCA CGTTACGTGT CTTCGATGCA 
GACGTGGTGC CAGCCTCCGG GGAGCTCCTG AGACGGTACA CGAACACGCT GCTCTCTGGG 
GACCCCAAGG CCCTCCGGAC CTTCCGAGTG GAGCACGCGC CCAATGAGAC CTTGGCCCAG 
GCCAACGGCA GCTTTGTGAG GGCAACCGTG CATGACTACA AGCTGGTTCT CAACCGGAGC 
CTCCCCATCT CGGAGAGCTG GGCTGTGCAG CTGGCCGTGC TGGTCAACGA CTCAGACTTC 
CAGGGCCCAG GGGAGGGTGT CGTCCTCCTC CACTTCAACG TGTCCGTGCT GCCCGTCAGC 
CTGCGCCTGC CCAGCGCCTA CTCCTTCTCC GTGAGCAGGC GGGCCCACCG CTTCGCCCAG 
ATCGGGAAAG TCTGCGTGGA AAACTGCCAG GAGTTCAGCG GCATCCAAGT GCAGTACAGG 
CTGCAGCCCT CCAGCGCCAA CTGCAGCGCC CTGGGGATGG TCACCTCGGC TGAGGACACC 
TCGGGGACCC TGTTCGTGAA TGACATAGAG GCCCTGCAGC GGCCCGAGTG TGCCCAGCTC 
CAGTACACGG TGGTGGCCGC CGAGAAACCA ACCCGCCAGC AAGCCCAGGC CCCGCTGCTC 
GTTAGCGTGG AAGGGATATA CGTGCCTGAG GAGCCTGGCT GCCCCCTGTC CTGCGCGGTC 
AGCAAGAGGC GGCCCGAATG TGAGGAATGC GGCGGCCTGG GCTCTCTAAC GGGCAGGTGC 
GAGTGGAGAC AGGGAGACGG CAAAGGGATC ACCAGGAACT TCTCCACCTG CTCCCCCAGC 
ATCAGGACCT GCCCCGATGG CCACTGTGAC GCCGTGGAGA GCAGAGACCC CAACATCTGC 
CCCCAGGACT GCCTCCACTC CCTGTGTGAT GAGCTCTGCC GCACAGTGAT CACGGCGGCC 
GTGCTCTTCT CCTTCATCGT GTCTGTGCTG CTGTCCACGT TCTGCATCCA CCGCTACCAC 
AAGAACGCCC ACAAGCCGCC CATTGCCTCG GCCGAGATGA CCTTCCGCCG GCCCGCCCAA 
GCCTTCCCGG TCAGCTACTC CTCGTCTGGC GCCCGCCGGC CCTCTCTGGA CTCCATGGAG 
AACCAGGTCT CGGTGGACAC CTTCAAGATC CCGGAGGATC CCAAGTGGGA ATTCCCTCGG 
AAGAACTTGG TGCTTGGAAA AACTCTGGGA GAAGGCGAAT TTGGAAAAGT GGTCAAGGCC 
ACAGCCTTCC GGCTGAAAGG CAAAGCGGGG TACACGACTG TGGCTGTGAA GATGCTGAAA 
GAGAATGCCT CCCCAAGCGA GCTGCGGGAC CTGCTATCAG AGTTCAACCT CCTGAAGCAG 
GTCAACCACC CGCAAGTCAT CAAGCTGTAC GGGGCCTGCA GCCAGGACGG GCCACTGCTC 
CTCATCGTGG AGTATGCCAA GTACGGCTCC CTGCGGGGCT TCCTCCGAGA GAGCCGCAAG 
GCGGGGCCTG GCTACGTGGG CAGCGGGGGC AGCCGCAATT CCAGTTACCT GGACAACCCG 
GAGGAGCGGG CCCTGACCAT GGGCGACCTC ATCTCCTTTG CCTGGCAGAT CTCACGAGGG 
ATGCAGTACC TGGCTGAGAT GAAGCTTGTC CATCGGGACT TGGCAGCCAG AAACGTCCTG 
GTAGCTGAGG GCCGCAGGAT GAAGATCTCA GATTTTGGTC TGTCCCGAGA TGTTTATGAA 
GAGGATTCCT ATGTGAAGAG GAGCAAGGGT CGGATCCCAG TCAAGTGGAT GGCCATTGAG 
TCCCTCTTCG ATCATATCTA TACCACCCAG AGTGATGTGT GGTCCTTTGG CGTTCTGCTG 
TGGGAGATTG TGACCCTGGG CGGCAACCCC TACCCTGGGA TTCCTCCAGA GCGGCTCTTC 
AACCTTCTGA AGACAGGCTA CCGGATGGAG AGGCCGGACA ACTGCAGTGA GGAGATGTAC 
AATCTGATGC TGCAGTGCTG GAAGCAGGAA CCAGACAAGA GGCCAGTGTT TGCCGACATC 
AGCAAAGACC TGGATAAGAT GATGGTTAAG AGCAGAGACT ACCTGGACCT GGCCGCGTCC 
ACCCCATCTG ACTCCCTGCT GTATGACGAC GGCCTGTCGG AGGAGGAGAC GCCCCTGGTG 
GACTGTAATA ACGCTCCCCT CCCTCGCACC CTCCCCTCCA CGTGGATTGA AAACAAACTC 
TATGGCATGT CAGACCCGAA CTGGCCTGAA GAGAGTCCTG TACCACTCAC GAGAGCCGCT 
GGCACCAACA TTGGGTGTCC AAGATATGCA AATGATAGTG TATATGCTAA CTGGATGGTT 
TCACCCTCAG CGGCACAATT AATGGACGCG TTTGATAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842421.1
CDS	216..3437
Translation	MAKATASTVGLRLLLLLLPLLGEAPLGLYFSRDAYWEKLYVDQPAGTPLLYVHALRDVPEEVPSFRLGQHLYGAYRTRLHENDWVRIQEDTGLLYLNQSLDHSSWEKLNIRNGGFPLLTVYLWVFLSPASVREGECQWPGCARVYFSVINASFPACGALKPRELCFPESGLSFRIRENRPPGTFHQFRLLPVQFLCPNVSVAYRLLGGESLPFRCDPDSLEVSTRWALDREQREKYELVAACTVRVGAREEEVMVPFPVTVYDEDDSAPTFLGGVDSASAVVEFKRKEGTVVATLRVFDADVVPASGELLRRYTNTLLSGDPKALRTFRVEHAPNETLAQANGSFVRATVHDYKLVLNRSLPISESWAVQLAVLVNDSDFQGPGEGVVLLHFNVSVLPVSLRLPSAYSFSVSRRAHRFAQIGKVCVENCQEFSGIQVQYRLQPSSANCSALGMVTSAEDTSGTLFVNDIEALQRPECAQLQYTVVAAEKPTRQQAQAPLLVSVEGIYVPEEPGCPLSCAVSKRRPECEECGGLGSLTGRCEWRQGDGKGITRNFSTCSPSIRTCPDGHCDAVESRDPNICPQDCLHSLCDELCRTVITAAVLFSFIVSVLLSTFCIHRYHKNAHKPPIASAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDTFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASPSELRDLLSEFNLLKQVNHPQVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKAGPGYVGSGGSRNSSYLDNPEERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRRMKISDFGLSRDVYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGYRMERPDNCSEEMYNLMLQCWKQEPDKRPVFADISKDLDKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNNAPLPRTLPSTWIENKLYGMSDPNWPEESPVPLTRAAGTNIGCPRYANDSVYANWMVSPSAAQLMDAFDS

Target ORF information:

RefSeq Version	XM_024986653.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ret proto-oncogene (RET), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986653.1

ORF Insert Sequence:

ATGGCGAAGG CGACGGCCAG CACCGTGGGG CTGCGGTTGC TGCTTCTGCT GCTGCCCCTG 
CTCGGCGAAG CCCCGCTGGG ACTCTACTTC TCCAGGGATG CTTACTGGGA GAAGCTATAC 
GTGGACCAGC CGGCCGGCAC GCCCCTGCTC TATGTCCACG CCCTGCGGGA CGTGCCGGAG 
GAGGTGCCCA GCTTCCGCCT GGGCCAGCAC CTCTACGGCG CCTACCGCAC GCGGCTGCAC 
GAGAACGACT GGGTCCGCAT CCAGGAGGAC ACGGGGCTTC TCTACCTTAA CCAGAGCCTG 
GACCACAGCT CCTGGGAGAA GCTCAACATC CGCAACGGCG GCTTCCCACT GCTTACCGTC 
TATCTCTGGG TCTTCCTGTC ACCCGCGTCC GTTCGTGAGG GCGAGTGCCA GTGGCCGGGC 
TGTGCCCGTG TGTACTTCTC CGTCATCAAT GCCTCCTTCC CAGCTTGCGG CGCCCTCAAA 
CCCCGGGAGC TCTGCTTCCC CGAGTCAGGC CTGTCCTTCC GCATCCGGGA GAACAGGCCC 
CCCGGCACCT TCCACCAGTT CCGCCTGCTG CCCGTGCAGT TCCTCTGCCC CAACGTCAGC 
GTGGCCTACA GGCTCCTGGG AGGTGAGAGT CTGCCTTTCC GCTGCGATCC GGACAGCCTG 
GAGGTGAGCA CGCGCTGGGC CCTGGACCGC GAGCAGCGGG AGAAGTATGA GCTGGTGGCC 
GCGTGCACGG TTCGCGTGGG CGCGCGCGAG GAGGAGGTGA TGGTGCCCTT CCCCGTGACC 
GTGTACGACG AGGACGACTC TGCGCCCACC TTCCTCGGGG GCGTCGACAG CGCCAGCGCC 
GTGGTGGAGT TCAAGCGGAA GGAGGGCACA GTGGTAGCCA CGTTACGTGT CTTCGATGCA 
GACGTGGTGC CAGCCTCCGG GGAGCTCCTG AGACGGTACA CGAACACGCT GCTCTCTGGG 
GACCCCAAGG CCCTCCGGAC CTTCCGAGTG GAGCACGCGC CCAATGAGAC CTTGGCCCAG 
GCCAACGGCA GCTTTGTGAG GGCAACCGTG CATGACTACA AGCTGGTTCT CAACCGGAGC 
CTCCCCATCT CGGAGAGCTG GGCTGTGCAG CTGGCCGTGC TGGTCAACGA CTCAGACTTC 
CAGGGCCCAG GGGAGGGTGT CGTCCTCCTC CACTTCAACG TGTCCGTGCT GCCCGTCAGC 
CTGCGCCTGC CCAGCGCCTA CTCCTTCTCC GTGAGCAGGC GGGCCCACCG CTTCGCCCAG 
ATCGGGAAAG TCTGCGTGGA AAACTGCCAG GAGTTCAGCG GCATCCAAGT GCAGTACAGG 
CTGCAGCCCT CCAGCGCCAA CTGCAGCGCC CTGGGGATGG TCACCTCGGC TGAGGACACC 
TCGGGGACCC TGTTCGTGAA TGACATAGAG GCCCTGCAGC GGCCCGAGTG TGCCCAGCTC 
CAGTACACGG TGGTGGCCGC CGAGAAACCA ACCCGCCAGC AAGCCCAGGC CCCGCTGCTC 
GTTAGCGTGG AAGGGATATA CGTGCCTGAG GAGCCTGGCT GCCCCCTGTC CTGCGCGGTC 
AGCAAGAGGC GGCCCGAATG TGAGGAATGC GGCGGCCTGG GCTCTCTAAC GGGCAGGTGC 
GAGTGGAGAC AGGGAGACGG CAAAGGGATC ACCAGGAACT TCTCCACCTG CTCCCCCAGC 
ATCAGGACCT GCCCCGATGG CCACTGTGAC GCCGTGGAGA GCAGAGACCC CAACATCTGC 
CCCCAGGACT GCCTCCACTC CCTGTGTGAT GAGCTCTGCC GCACAGTGAT CACGGCGGCC 
GTGCTCTTCT CCTTCATCGT GTCTGTGCTG CTGTCCACGT TCTGCATCCA CCGCTACCAC 
AAGAACGCCC ACAAGCCGCC CATTGCCTCG GCCGAGATGA CCTTCCGCCG GCCCGCCCAA 
GCCTTCCCGG TCAGCTACTC CTCGTCTGGC GCCCGCCGGC CCTCTCTGGA CTCCATGGAG 
AACCAGGTCT CGGTGGACAC CTTCAAGATC CCGGAGGATC CCAAGTGGGA ATTCCCTCGG 
AAGAACTTGG TGCTTGGAAA AACTCTGGGA GAAGGCGAAT TTGGAAAAGT GGTCAAGGCC 
ACAGCCTTCC GGCTGAAAGG CAAAGCGGGG TACACGACTG TGGCTGTGAA GATGCTGAAA 
GAGAATGCCT CCCCAAGCGA GCTGCGGGAC CTGCTATCAG AGTTCAACCT CCTGAAGCAG 
GTCAACCACC CGCAAGTCAT CAAGCTGTAC GGGGCCTGCA GCCAGGACGG GCCACTGCTC 
CTCATCGTGG AGTATGCCAA GTACGGCTCC CTGCGGGGCT TCCTCCGAGA GAGCCGCAAG 
GCGGGGCCTG GCTACGTGGG CAGCGGGGGC AGCCGCAATT CCAGTTACCT GGACAACCCG 
GAGGAGCGGG CCCTGACCAT GGGCGACCTC ATCTCCTTTG CCTGGCAGAT CTCACGAGGG 
ATGCAGTACC TGGCTGAGAT GAAGCTTGTC CATCGGGACT TGGCAGCCAG AAACGTCCTG 
GTAGCTGAGG GCCGCAGGAT GAAGATCTCA GATTTTGGTC TGTCCCGAGA TGTTTATGAA 
GAGGATTCCT ATGTGAAGAG GAGCAAGGGT CGGATCCCAG TCAAGTGGAT GGCCATTGAG 
TCCCTCTTCG ATCATATCTA TACCACCCAG AGTGATGTGT GGTCCTTTGG CGTTCTGCTG 
TGGGAGATTG TGACCCTGGG CGGCAACCCC TACCCTGGGA TTCCTCCAGA GCGGCTCTTC 
AACCTTCTGA AGACAGGCTA CCGGATGGAG AGGCCGGACA ACTGCAGTGA GGAGATGTAC 
AATCTGATGC TGCAGTGCTG GAAGCAGGAA CCAGACAAGA GGCCAGTGTT TGCCGACATC 
AGCAAAGACC TGGATAAGAT GATGGTTAAG AGCAGAGACT ACCTGGACCT GGCCGCGTCC 
ACCCCATCTG ACTCCCTGCT GTATGACGAC GGCCTGTCGG AGGAGGAGAC GCCCCTGGTG 
GACTGTAATA ACGCTCCCCT CCCTCGCACC CTCCCCTCCA CGTGGATTGA AAACAAACTC 
TATGGCATGT CAGACCCGAA CTGGCCTGAA GAGAGTCCTG TACCACTCAC GAGAGCCGCT 
GGCACCAACA TTGGGTGTCC AAGATATGCA AATGATAGTG TATATGCTAA CTGGATGGTT 
TCACCCTCAG CGGCACAATT AATGGACGCG TTTGATAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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RET cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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