SKA1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SKA1
Entrez Gene ID	507703
Full Name	spindle and kinetochore associated complex subunit 1
General protein information	Preferred Names spindle and kinetochore associated complex subunit 1 Names spindle and kinetochore-associated protein 1 spindle and KT associated 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005224135.3	XP_005224192.1	spindle and kinetochore-associated protein 1 isoform X1
XM_005224135.4	XP_005224192.1	spindle and kinetochore-associated protein 1 isoform X2
XM_024984202.1	XP_024839970.1	spindle and kinetochore-associated protein 1 isoform X1
XM_024984203.1	XP_024839971.1	spindle and kinetochore-associated protein 1 isoform X2
XM_005224136.3	XP_005224193.1	spindle and kinetochore-associated protein 1 isoform X1
NM_001075327.2	NP_001068795.2	spindle and kinetochore-associated protein 1
XM_005224136.4	XP_005224193.1	spindle and kinetochore-associated protein 1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ska1	NP_079857.3
Pan troglodytes (chimpanzee)	SKA1	XP_512132.2
Macaca mulatta (Rhesus monkey)	SKA1	XP_001093574.1
Rattus norvegicus (Norway rat)	Ska1	NP_001099604.1
Xenopus tropicalis (tropical clawed frog)	ska1	NP_001119997.1
Homo sapiens (human)	SKA1	NP_001034624.1
Canis lupus familiaris (dog)	SKA1	XP_548812.1
Bos taurus (cattle)	SKA1	NP_001068795.2
Danio rerio (zebrafish)	ska1	NP_001002592.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mahé MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Drögemüller C, Williams JL
BMC genomics7283(2006 Nov)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SKA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SKA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa32513	NM_001075327.2 Latest version!	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa229853	XM_024984202.1 Latest version!	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa32513	XM_024984203.1 Latest version!	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa32513	XM_005224136.4 Latest version!	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa32513	XM_005224135.4 Latest version!	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OBa32513	XM_005224135.3	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa32513	XM_005224136.3	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SKA1 ( NM_001075327.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001068795.2 Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), mRNA.

Price & Availability↑

CloneID	OBa32513
Clone ID Related Accession (Same CDS sequence)	XM_005224135.3 , XM_005224135.4 , XM_024984203.1 , XM_005224136.3 , NM_001075327.2 , XM_005224136.4
Accession Version	NM_001075327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Bos taurus(cattle)
Product	spindle and kinetochore-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BT026543.1, CK971393.1 and CO881184.1. On Jan 3, 2008 this sequence version replaced NM_001075327.1. ##Evidence-Data-START## Transcript exon combination :: CK971393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145422 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068795.2
CDS	165..929
Translation	MASDLEQLCSHINEKIGNIKRTLSLRNCGQEPTLKTILNKIGDEIIVVNELLNKLELEIQYQEQTNSSLKELFESLEEDYKDVEHLKENIPPHLPQVTVTQNFVNGSDLDPEEPVKVEEPAPTKKPPKEQRSIKEMPFITSDEFNGIPAYMKSRLTYCHINDVIKEINKAVVSKYKILHQPKKSMSSVARNLYHRFIDEETKETKGHYFVVEADIKEFTTLKVDKRFHGILNILRHCRRLSEVRGKGLTRYVIT

Target ORF information:

RefSeq Version	NM_001075327.2
Organism	Bos taurus(cattle)
Definition	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075327.2

ORF Insert Sequence:

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKA1 ( XM_024984202.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839970.1 Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229853
Clone ID Related Accession (Same CDS sequence)	XM_024984202.1
Accession Version	XM_024984202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	spindle and kinetochore-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATCC TGCGTCCCTC GGCGATCAGG GCGCGCCAGG AGCTGGAGGG GCCTCAGCTC 
AGCAACAAGA GGCCCGTGCT GTCCCAGAGC CCGGCACTCC CCTGGGGGGA CTTCGCTACA 
CGCTACCCAC CGCCAGGAGG CTTAAAGATG GCCTCAGATC TGGAACAATT ATGCTCTCAT 
ATTAATGAAA AGATCGGCAA TATTAAAAGA ACTCTGTCAT TGAGAAATTG TGGGCAAGAA 
CCTACCTTGA AGACCATTTT AAATAAAATA GGAGATGAGA TCATTGTAGT AAATGAACTT 
CTAAATAAAT TGGAATTGGA AATTCAGTAT CAAGAACAAA CCAACAGTTC CCTCAAGGAA 
CTCTTTGAAT CTCTCGAAGA AGATTATAAA GATGTGGAGC ATCTCAAAGA AAACATCCCT 
CCCCATTTGC CTCAAGTAAC AGTTACCCAG AACTTTGTTA ACGGATCAGA TCTTGACCCT 
GAAGAACCGG TCAAAGTTGA GGAACCTGCC CCCACAAAGA AGCCTCCCAA AGAACAAAGA 
AGTATTAAAG AAATGCCATT TATAACTTCT GATGAGTTTA ATGGCATTCC TGCGTACATG 
AAATCCCGCT TAACCTATTG TCACATTAAC GATGTTATTA AAGAAATCAA CAAGGCAGTT 
GTTAGTAAAT ATAAGATCCT ACATCAACCA AAAAAGTCTA TGAGTTCTGT GGCCAGAAAT 
CTCTATCACA GATTTATTGA CGAAGAAACG AAGGAAACCA AAGGTCATTA TTTTGTAGTG 
GAAGCTGACA TAAAGGAGTT CACAACTTTG AAAGTCGACA AGAGGTTTCA TGGGATACTG 
AATATTTTAC GACACTGCCG GAGGCTATCA GAGGTCCGAG GGAAAGGGCT TACCCGATAT 
GTTATAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839970.1
CDS	32..943
Translation	MEILRPSAIRARQELEGPQLSNKRPVLSQSPALPWGDFATRYPPPGGLKMASDLEQLCSHINEKIGNIKRTLSLRNCGQEPTLKTILNKIGDEIIVVNELLNKLELEIQYQEQTNSSLKELFESLEEDYKDVEHLKENIPPHLPQVTVTQNFVNGSDLDPEEPVKVEEPAPTKKPPKEQRSIKEMPFITSDEFNGIPAYMKSRLTYCHINDVIKEINKAVVSKYKILHQPKKSMSSVARNLYHRFIDEETKETKGHYFVVEADIKEFTTLKVDKRFHGILNILRHCRRLSEVRGKGLTRYVIT

Target ORF information:

RefSeq Version	XM_024984202.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984202.1

ORF Insert Sequence:

ATGGAAATCC TGCGTCCCTC GGCGATCAGG GCGCGCCAGG AGCTGGAGGG GCCTCAGCTC 
AGCAACAAGA GGCCCGTGCT GTCCCAGAGC CCGGCACTCC CCTGGGGGGA CTTCGCTACA 
CGCTACCCAC CGCCAGGAGG CTTAAAGATG GCCTCAGATC TGGAACAATT ATGCTCTCAT 
ATTAATGAAA AGATCGGCAA TATTAAAAGA ACTCTGTCAT TGAGAAATTG TGGGCAAGAA 
CCTACCTTGA AGACCATTTT AAATAAAATA GGAGATGAGA TCATTGTAGT AAATGAACTT 
CTAAATAAAT TGGAATTGGA AATTCAGTAT CAAGAACAAA CCAACAGTTC CCTCAAGGAA 
CTCTTTGAAT CTCTCGAAGA AGATTATAAA GATGTGGAGC ATCTCAAAGA AAACATCCCT 
CCCCATTTGC CTCAAGTAAC AGTTACCCAG AACTTTGTTA ACGGATCAGA TCTTGACCCT 
GAAGAACCGG TCAAAGTTGA GGAACCTGCC CCCACAAAGA AGCCTCCCAA AGAACAAAGA 
AGTATTAAAG AAATGCCATT TATAACTTCT GATGAGTTTA ATGGCATTCC TGCGTACATG 
AAATCCCGCT TAACCTATTG TCACATTAAC GATGTTATTA AAGAAATCAA CAAGGCAGTT 
GTTAGTAAAT ATAAGATCCT ACATCAACCA AAAAAGTCTA TGAGTTCTGT GGCCAGAAAT 
CTCTATCACA GATTTATTGA CGAAGAAACG AAGGAAACCA AAGGTCATTA TTTTGTAGTG 
GAAGCTGACA TAAAGGAGTT CACAACTTTG AAAGTCGACA AGAGGTTTCA TGGGATACTG 
AATATTTTAC GACACTGCCG GAGGCTATCA GAGGTCCGAG GGAAAGGGCT TACCCGATAT 
GTTATAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKA1 ( XM_005224135.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224192.1 Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32513
Clone ID Related Accession (Same CDS sequence)	XM_005224135.3 , XM_005224135.4 , XM_024984203.1 , XM_005224136.3 , NM_001075327.2 , XM_005224136.4
Accession Version	XM_005224135.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	spindle and kinetochore-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224192.1
CDS	477..1241
Translation	MASDLEQLCSHINEKIGNIKRTLSLRNCGQEPTLKTILNKIGDEIIVVNELLNKLELEIQYQEQTNSSLKELFESLEEDYKDVEHLKENIPPHLPQVTVTQNFVNGSDLDPEEPVKVEEPAPTKKPPKEQRSIKEMPFITSDEFNGIPAYMKSRLTYCHINDVIKEINKAVVSKYKILHQPKKSMSSVARNLYHRFIDEETKETKGHYFVVEADIKEFTTLKVDKRFHGILNILRHCRRLSEVRGKGLTRYVIT

Target ORF information:

RefSeq Version	XM_005224135.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224135.3

ORF Insert Sequence:

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKA1 ( XM_005224135.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224192.1 Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32513
Clone ID Related Accession (Same CDS sequence)	XM_005224135.3 , XM_005224135.4 , XM_024984203.1 , XM_005224136.3 , NM_001075327.2 , XM_005224136.4
Accession Version	XM_005224135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	spindle and kinetochore-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224135.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224192.1
CDS	521..1285
Translation	MASDLEQLCSHINEKIGNIKRTLSLRNCGQEPTLKTILNKIGDEIIVVNELLNKLELEIQYQEQTNSSLKELFESLEEDYKDVEHLKENIPPHLPQVTVTQNFVNGSDLDPEEPVKVEEPAPTKKPPKEQRSIKEMPFITSDEFNGIPAYMKSRLTYCHINDVIKEINKAVVSKYKILHQPKKSMSSVARNLYHRFIDEETKETKGHYFVVEADIKEFTTLKVDKRFHGILNILRHCRRLSEVRGKGLTRYVIT

Target ORF information:

RefSeq Version	XM_005224135.4
Organism	Bos taurus(cattle)
Definition	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224135.4

ORF Insert Sequence:

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKA1 ( XM_024984203.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839971.1 Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa32513
Clone ID Related Accession (Same CDS sequence)	XM_005224135.3 , XM_005224135.4 , XM_024984203.1 , XM_005224136.3 , NM_001075327.2 , XM_005224136.4
Accession Version	XM_024984203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	spindle and kinetochore-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839971.1
CDS	788..1552
Translation	MASDLEQLCSHINEKIGNIKRTLSLRNCGQEPTLKTILNKIGDEIIVVNELLNKLELEIQYQEQTNSSLKELFESLEEDYKDVEHLKENIPPHLPQVTVTQNFVNGSDLDPEEPVKVEEPAPTKKPPKEQRSIKEMPFITSDEFNGIPAYMKSRLTYCHINDVIKEINKAVVSKYKILHQPKKSMSSVARNLYHRFIDEETKETKGHYFVVEADIKEFTTLKVDKRFHGILNILRHCRRLSEVRGKGLTRYVIT

Target ORF information:

RefSeq Version	XM_024984203.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984203.1

ORF Insert Sequence:

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKA1 ( XM_005224136.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224193.1 Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32513
Clone ID Related Accession (Same CDS sequence)	XM_005224135.3 , XM_005224135.4 , XM_024984203.1 , XM_005224136.3 , NM_001075327.2 , XM_005224136.4
Accession Version	XM_005224136.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	spindle and kinetochore-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224193.1
CDS	336..1100
Translation	MASDLEQLCSHINEKIGNIKRTLSLRNCGQEPTLKTILNKIGDEIIVVNELLNKLELEIQYQEQTNSSLKELFESLEEDYKDVEHLKENIPPHLPQVTVTQNFVNGSDLDPEEPVKVEEPAPTKKPPKEQRSIKEMPFITSDEFNGIPAYMKSRLTYCHINDVIKEINKAVVSKYKILHQPKKSMSSVARNLYHRFIDEETKETKGHYFVVEADIKEFTTLKVDKRFHGILNILRHCRRLSEVRGKGLTRYVIT

Target ORF information:

RefSeq Version	XM_005224136.3
Organism	Bos taurus(cattle)
Definition	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224136.3

ORF Insert Sequence:

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKA1 ( XM_005224136.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224193.1 Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa32513
Clone ID Related Accession (Same CDS sequence)	XM_005224135.3 , XM_005224135.4 , XM_024984203.1 , XM_005224136.3 , NM_001075327.2 , XM_005224136.4
Accession Version	XM_005224136.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	spindle and kinetochore-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224136.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224193.1
CDS	325..1089
Translation	MASDLEQLCSHINEKIGNIKRTLSLRNCGQEPTLKTILNKIGDEIIVVNELLNKLELEIQYQEQTNSSLKELFESLEEDYKDVEHLKENIPPHLPQVTVTQNFVNGSDLDPEEPVKVEEPAPTKKPPKEQRSIKEMPFITSDEFNGIPAYMKSRLTYCHINDVIKEINKAVVSKYKILHQPKKSMSSVARNLYHRFIDEETKETKGHYFVVEADIKEFTTLKVDKRFHGILNILRHCRRLSEVRGKGLTRYVIT

Target ORF information:

RefSeq Version	XM_005224136.4
Organism	Bos taurus(cattle)
Definition	Bos taurus spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224136.4

ORF Insert Sequence:

ATGGCCTCAG ATCTGGAACA ATTATGCTCT CATATTAATG AAAAGATCGG CAATATTAAA 
AGAACTCTGT CATTGAGAAA TTGTGGGCAA GAACCTACCT TGAAGACCAT TTTAAATAAA 
ATAGGAGATG AGATCATTGT AGTAAATGAA CTTCTAAATA AATTGGAATT GGAAATTCAG 
TATCAAGAAC AAACCAACAG TTCCCTCAAG GAACTCTTTG AATCTCTCGA AGAAGATTAT 
AAAGATGTGG AGCATCTCAA AGAAAACATC CCTCCCCATT TGCCTCAAGT AACAGTTACC 
CAGAACTTTG TTAACGGATC AGATCTTGAC CCTGAAGAAC CGGTCAAAGT TGAGGAACCT 
GCCCCCACAA AGAAGCCTCC CAAAGAACAA AGAAGTATTA AAGAAATGCC ATTTATAACT 
TCTGATGAGT TTAATGGCAT TCCTGCGTAC ATGAAATCCC GCTTAACCTA TTGTCACATT 
AACGATGTTA TTAAAGAAAT CAACAAGGCA GTTGTTAGTA AATATAAGAT CCTACATCAA 
CCAAAAAAGT CTATGAGTTC TGTGGCCAGA AATCTCTATC ACAGATTTAT TGACGAAGAA 
ACGAAGGAAA CCAAAGGTCA TTATTTTGTA GTGGAAGCTG ACATAAAGGA GTTCACAACT 
TTGAAAGTCG ACAAGAGGTT TCATGGGATA CTGAATATTT TACGACACTG CCGGAGGCTA 
TCAGAGGTCC GAGGGAAAGG GCTTACCCGA TATGTTATAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mahé MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Drögemüller C,Williams JL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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SKA1 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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